AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88743

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88743.t1 MTR_5g069710 95.765 307 13 0 1 307 1 307 0.0 615
MS.gene88743.t1 MTR_3g462790 66.261 329 82 8 1 307 1 322 5.31e-149 421
MS.gene88743.t1 MTR_7g067080 58.654 312 114 8 1 303 7 312 4.23e-117 340
MS.gene88743.t1 MTR_5g081860 62.871 202 68 3 4 203 2 198 2.64e-86 260
MS.gene88743.t1 MTR_1g111830 50.282 177 79 2 27 200 22 192 2.71e-56 182
MS.gene88743.t1 MTR_4g111975 44.538 238 94 5 29 233 8 240 4.63e-56 184
MS.gene88743.t1 MTR_8g063600 44.550 211 107 4 22 230 3 205 7.64e-51 170
MS.gene88743.t1 MTR_8g063870 41.341 179 84 3 23 199 2 161 8.30e-42 145
MS.gene88743.t1 MTR_4g100630 58.182 110 36 2 90 196 57 159 7.89e-32 121
MS.gene88743.t1 MTR_2g100930 73.239 71 19 0 127 197 104 174 1.35e-31 121
MS.gene88743.t1 MTR_6g092540 52.632 114 49 1 126 239 91 199 4.09e-31 118
MS.gene88743.t1 MTR_8g101650 43.038 158 62 5 118 253 91 242 1.50e-30 116
MS.gene88743.t1 MTR_4g015130 80.645 62 12 0 142 203 3 64 4.57e-30 109
MS.gene88743.t1 MTR_5g037080 70.513 78 23 0 126 203 96 173 1.30e-28 112
MS.gene88743.t1 MTR_4g107230 66.667 81 27 0 122 202 74 154 8.17e-27 107
MS.gene88743.t1 MTR_3g104370 48.780 123 49 4 129 240 88 207 2.93e-26 103
MS.gene88743.t1 MTR_5g088010 53.846 91 38 2 124 211 52 141 5.51e-25 100
MS.gene88743.t1 MTR_1g033620 59.551 89 35 1 148 236 21 108 1.02e-23 97.4
MS.gene88743.t1 MTR_0036s0260 64.179 67 24 0 132 198 28 94 2.01e-23 95.9
MS.gene88743.t1 MTR_5g088060 59.259 81 29 2 134 211 274 353 4.12e-23 99.4
MS.gene88743.t1 MTR_5g488160 59.259 81 29 2 134 211 274 353 4.12e-23 99.4
MS.gene88743.t1 MTR_2g090305 64.179 67 24 0 132 198 28 94 9.07e-23 93.6
MS.gene88743.t1 MTR_5g027570 55.814 86 35 2 128 211 88 172 1.36e-22 94.4
MS.gene88743.t1 MTR_5g075760 35.882 170 89 7 23 188 7 160 6.70e-22 92.0
MS.gene88743.t1 MTR_5g075790 35.088 171 88 6 23 188 16 168 2.68e-21 90.5
MS.gene88743.t1 MTR_1g033600 35.338 133 69 2 26 146 9 136 1.53e-20 86.3
MS.gene88743.t1 MTR_5g027550 56.322 87 34 2 128 211 73 158 2.90e-20 87.8
MS.gene88743.t1 MTR_1g022290 49.315 73 37 0 24 96 2 74 7.71e-20 82.8
MS.gene88743.t1 MTR_8g077380 51.389 72 34 1 25 96 1 71 6.37e-19 79.7
MS.gene88743.t1 MTR_8g077390 51.389 72 34 1 25 96 1 71 6.37e-19 79.7
MS.gene88743.t1 MTR_8g077360 51.389 72 34 1 25 96 1 71 6.61e-19 79.7
MS.gene88743.t1 MTR_1g048660 56.250 64 28 0 133 196 13 76 7.14e-19 80.1
MS.gene88743.t1 MTR_3g116720 52.308 65 31 0 29 93 16 80 7.23e-19 79.7
MS.gene88743.t1 MTR_3g111880 54.545 66 30 0 26 91 4 69 1.44e-18 79.3
MS.gene88743.t1 MTR_5g020170 46.875 64 34 0 28 91 18 81 1.58e-18 79.3
MS.gene88743.t1 MTR_6g004250 47.297 74 39 0 24 97 5 78 8.99e-18 77.0
MS.gene88743.t1 MTR_3g013440 45.455 88 36 2 24 110 5 81 5.18e-16 72.4
MS.gene88743.t1 MTR_8g077420 49.231 65 32 1 25 89 1 64 1.03e-15 71.2
MS.gene88743.t1 MTR_5g020540 50.820 61 29 1 29 89 4 63 6.46e-15 68.6
MS.gene88743.t1 MTR_3g070110 37.984 129 67 2 80 201 49 171 1.24e-14 72.0
MS.gene88743.t1 MTR_7g089210 53.333 60 28 0 32 91 15 74 2.32e-14 67.8
MS.gene88743.t1 MTR_1g083180 52.055 73 30 2 134 202 63 134 2.78e-13 67.4
MS.gene88743.t1 MTR_3g111920 44.444 63 35 0 29 91 3 65 3.17e-12 62.0
MS.gene88743.t1 MTR_6g043480 46.774 62 32 1 117 178 93 153 8.85e-12 64.3
MS.gene88743.t1 MTR_8g072990 43.056 72 36 2 129 196 97 167 1.95e-11 63.2
MS.gene88743.t1 MTR_6g043490 53.191 47 22 0 132 178 107 153 2.19e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88743.t1 AT2G38090 65.865 208 58 2 5 201 5 210 9.77e-99 293
MS.gene88743.t1 AT5G58900 68.224 214 53 4 5 204 1 213 2.96e-95 283
MS.gene88743.t1 AT5G01200 61.500 200 62 5 16 201 17 215 2.68e-78 239
MS.gene88743.t1 AT5G05790 61.275 204 68 5 5 201 1 200 6.77e-78 239
MS.gene88743.t1 AT5G05790 61.275 204 68 5 5 201 1 200 6.77e-78 239
MS.gene88743.t1 AT3G11280 67.052 173 48 2 29 201 33 196 8.23e-78 238
MS.gene88743.t1 AT3G11280 67.052 173 48 2 29 201 33 196 8.23e-78 238
MS.gene88743.t1 AT1G49010 51.232 203 72 5 23 200 2 202 7.34e-55 181
MS.gene88743.t1 AT5G08520 50.273 183 86 3 22 200 5 186 5.36e-54 178
MS.gene88743.t1 AT5G04760 41.892 222 116 3 26 244 3 214 1.15e-50 167
MS.gene88743.t1 AT5G23650 41.538 195 88 3 18 197 2 185 5.75e-42 148
MS.gene88743.t1 AT5G47390 60.185 108 35 3 92 197 60 161 1.02e-31 121
MS.gene88743.t1 AT3G10580 41.573 178 80 7 23 197 2 158 1.06e-31 119
MS.gene88743.t1 AT3G10580 41.573 178 80 7 23 197 2 158 1.71e-31 119
MS.gene88743.t1 AT4G09450 39.572 187 93 5 28 214 6 172 1.61e-30 114
MS.gene88743.t1 AT5G61620 50.862 116 57 0 127 242 103 218 2.24e-28 111
MS.gene88743.t1 AT5G56840 50.000 104 51 1 98 200 57 160 3.29e-27 106
MS.gene88743.t1 AT1G70000 70.833 72 21 0 126 197 92 163 1.06e-26 105
MS.gene88743.t1 AT1G70000 70.833 72 21 0 126 197 92 163 1.06e-26 105
MS.gene88743.t1 AT3G16350 72.222 72 20 0 127 198 132 203 1.81e-25 105
MS.gene88743.t1 AT1G74840 70.833 72 21 0 127 198 91 162 6.05e-25 100
MS.gene88743.t1 AT1G74840 70.833 72 21 0 127 198 91 162 8.63e-25 100
MS.gene88743.t1 AT1G19000 52.727 110 48 1 128 237 98 203 1.97e-24 100
MS.gene88743.t1 AT1G19000 52.727 110 48 1 128 237 98 203 1.97e-24 100
MS.gene88743.t1 AT1G75250 55.714 70 31 0 24 93 7 76 1.82e-21 88.2
MS.gene88743.t1 AT1G75250 55.714 70 31 0 24 93 7 76 2.06e-21 87.4
MS.gene88743.t1 AT3G10590 35.912 181 98 6 26 197 4 175 4.82e-21 89.4
MS.gene88743.t1 AT2G21650 52.941 68 32 0 29 96 14 81 8.43e-18 77.8
MS.gene88743.t1 AT4G39250 49.231 65 33 0 29 93 14 78 1.23e-16 74.3
MS.gene88743.t1 AT1G19510 51.613 62 30 0 32 93 15 76 2.66e-15 70.9
MS.gene88743.t1 AT2G18328 52.542 59 28 0 33 91 15 73 5.10e-13 63.9
MS.gene88743.t1 AT4G36570 56.818 44 19 0 29 72 12 55 1.02e-11 59.7

Find 57 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTTGCCATACTTCTTT+AGG 0.307378 5.4:-54536148 None:intergenic
TATTTCATAAGACAACTTTC+AGG 0.314437 5.4:+54536242 MS.gene88743:CDS
TTAGATTGGGTGAACAGTTC+TGG 0.315891 5.4:+54535470 MS.gene88743:CDS
CCTTTCTTCCTTTCCTGCTC+AGG 0.322349 5.4:-54535542 None:intergenic
TTCACCCAATCTAAGGTAAA+AGG 0.324294 5.4:-54535461 None:intergenic
CTACTAGGCCTAAAGAAGTA+TGG 0.375050 5.4:+54536140 MS.gene88743:CDS
GCTGGGTTGATTCCAGTTCC+TGG 0.382171 5.4:+54535419 MS.gene88743:CDS
ACATCACCGACCGTCTTTCC+TGG 0.388704 5.4:-54535350 None:intergenic
AGAACTGTTCACCCAATCTA+AGG 0.390954 5.4:-54535468 None:intergenic
GATGTTTGTAATATAGAAGC+TGG 0.393501 5.4:+54535401 MS.gene88743:CDS
AACATTATTCACATGCATAA+AGG 0.404368 5.4:+54536618 MS.gene88743:CDS
TGATAAGATGAAGTGGGAAA+TGG 0.405855 5.4:+54535166 None:intergenic
AAGTTTGTTGGTGCTGCTGC+TGG 0.410002 5.4:-54536397 None:intergenic
TGATGTGATGAGACAATATA+AGG 0.419767 5.4:+54535367 MS.gene88743:CDS
AGAAGTATGGCAAAGGTGAT+TGG 0.420618 5.4:+54536153 MS.gene88743:CDS
GTTGTGATGAACGTTGCATT+TGG 0.427349 5.4:-54536598 None:intergenic
CAAGACCAAAATCTTCAAAA+AGG 0.427744 5.4:+54536536 MS.gene88743:CDS
TGGTGAAGTGGATCTATTAC+CGG 0.443254 5.4:-54536351 None:intergenic
GCTATCTTCCATACACCAAT+TGG 0.448071 5.4:-54535222 None:intergenic
TTGTGTTCCTCTTCAGTCCA+TGG 0.459712 5.4:-54535569 None:intergenic
CATACACACAAAACTCCAAT+TGG 0.463612 5.4:+54535207 MS.gene88743:CDS
TATGATGAATTCAGAGGAAG+TGG 0.470044 5.4:+54535494 MS.gene88743:CDS
ATGAAAACTTGCTCGTGAGC+TGG 0.470697 5.4:-54536476 None:intergenic
GCATGGCTTGCTACTTGAGT+TGG 0.471843 5.4:-54536212 None:intergenic
GCTACTTTATGCCATCGATC+AGG 0.473748 5.4:-54535317 None:intergenic
GGAGTGTTATAACCAGGAAC+TGG 0.478409 5.4:-54535431 None:intergenic
ACAAAACTCCAATTGGTGTA+TGG 0.484625 5.4:+54535214 MS.gene88743:CDS
TCCTGCAGATTATTTCTACT+AGG 0.501666 5.4:+54536125 MS.gene88743:intron
TTAGAGCTCCTGAGCAGGAA+AGG 0.515723 5.4:+54535534 MS.gene88743:CDS
TCTAAGGTAAAAGGTGAAGT+AGG 0.517348 5.4:-54535452 None:intergenic
CCTGAGCAGGAAAGGAAGAA+AGG 0.521387 5.4:+54535542 MS.gene88743:CDS
TTTAGTTAGAGCTCCTGAGC+AGG 0.523673 5.4:+54535529 MS.gene88743:CDS
GAAAACTTGATAAGATGAAG+TGG 0.527680 5.4:+54535159 None:intergenic
CATAGAATTCTGTGGTGAAG+TGG 0.532652 5.4:-54536363 None:intergenic
GAATGATCAACAAAATGCTA+TGG 0.544650 5.4:+54536445 MS.gene88743:CDS
TTGTTGCCGTCGAAGTTTGT+TGG 0.545215 5.4:-54536409 None:intergenic
ATTTGGAAAACCATGTTATG+TGG 0.549103 5.4:-54536581 None:intergenic
CTCTTCTGGAGTCCATTGTG+TGG 0.588727 5.4:-54535249 None:intergenic
AAAGTAGCTGAAATGATACC+AGG 0.595280 5.4:+54535332 MS.gene88743:CDS
TTCATAAGACAACTTTCAGG+AGG 0.595999 5.4:+54536245 MS.gene88743:CDS
GTCTTCTTATCCACATAACA+TGG 0.600765 5.4:+54536571 MS.gene88743:CDS
ATGTTTGTAATATAGAAGCT+GGG 0.604659 5.4:+54535402 MS.gene88743:CDS
AGGAGTGCCATGGACTGAAG+AGG 0.610163 5.4:+54535562 MS.gene88743:CDS
AAAACTTGATAAGATGAAGT+GGG 0.612600 5.4:+54535160 None:intergenic
GGCCTAAAGAAGTATGGCAA+AGG 0.616426 5.4:+54536146 MS.gene88743:CDS
AAAGGAAGAAAGGAGTGCCA+TGG 0.621552 5.4:+54535552 MS.gene88743:CDS
TGAAATGATACCAGGAAAGA+CGG 0.622184 5.4:+54535340 MS.gene88743:CDS
TCTGGTTATGATGAATTCAG+AGG 0.623212 5.4:+54535488 MS.gene88743:CDS
TTTCAGGAGGAAAAGACAAG+AGG 0.628437 5.4:+54536258 MS.gene88743:CDS
ATAAAGATACACCTGATCGA+TGG 0.635123 5.4:+54535306 MS.gene88743:CDS
ATGATACCAGGAAAGACGGT+CGG 0.635685 5.4:+54535344 MS.gene88743:CDS
GAAGTAGGAGTGTTATAACC+AGG 0.644855 5.4:-54535437 None:intergenic
GCAGCACCAACAAACTTCGA+CGG 0.651047 5.4:+54536403 MS.gene88743:CDS
AAGATAGCATGACCACACAA+TGG 0.678472 5.4:+54535237 MS.gene88743:CDS
ACTTGTTCTTCAGAAGACAC+CGG 0.691433 5.4:+54536332 MS.gene88743:CDS
TCATAGGAGTTAAAACCATG+AGG 0.695559 5.4:-54536503 None:intergenic
GAAAGTAACATAGAATTCTG+TGG 0.702812 5.4:-54536371 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTATAAATATATTTAAA+GGG + chr5.4:54535872-54535891 MS.gene88743:intron 0.0%
!! TTATTTATAAATATATTTAA+AGG + chr5.4:54535871-54535890 MS.gene88743:intron 0.0%
!! AATAACAAAACAAATACAAA+AGG - chr5.4:54535607-54535626 None:intergenic 15.0%
!! TATAAATATATTTAAAGGGT+TGG + chr5.4:54535876-54535895 MS.gene88743:intron 15.0%
!!! TTAGTATTTTTTCTTTACTA+GGG + chr5.4:54535678-54535697 MS.gene88743:intron 15.0%
!! ATATCATATTAAGTTAGAGT+AGG + chr5.4:54536028-54536047 MS.gene88743:intron 20.0%
!! CTAACTTAATATGATATTTG+AGG - chr5.4:54536025-54536044 None:intergenic 20.0%
!! GTTAATCTTTCAGAAAAAAT+TGG + chr5.4:54536308-54536327 MS.gene88743:CDS 20.0%
!!! GTTAGTATTTTTTCTTTACT+AGG + chr5.4:54535677-54535696 MS.gene88743:intron 20.0%
!!! TTAAGAAAGTATAGGAATAA+AGG + chr5.4:54535802-54535821 MS.gene88743:intron 20.0%
! ATAAAGGATGGAATTTACAA+AGG + chr5.4:54535818-54535837 MS.gene88743:intron 25.0%
! ATGTTTGTAATATAGAAGCT+GGG + chr5.4:54535402-54535421 MS.gene88743:CDS 25.0%
! TAACTTAAGATAGTAACAGA+AGG - chr5.4:54536096-54536115 None:intergenic 25.0%
! TATTTCATAAGACAACTTTC+AGG + chr5.4:54536242-54536261 MS.gene88743:CDS 25.0%
!! TCAAATATTTTGTTCTCTTC+TGG - chr5.4:54535266-54535285 None:intergenic 25.0%
!! TTATGAAATACTTTTGAGCA+TGG - chr5.4:54536232-54536251 None:intergenic 25.0%
!!! ATGATATAAGTTGGTTTTGT+AGG + chr5.4:54536053-54536072 MS.gene88743:intron 25.0%
!!! GTTGGATATTTTCATTTTTC+AGG + chr5.4:54535894-54535913 MS.gene88743:intron 25.0%
!!! TCATTTTTCAGGTTGTAATA+AGG + chr5.4:54535905-54535924 MS.gene88743:intron 25.0%
!!! TGATATAAGTTGGTTTTGTA+GGG + chr5.4:54536054-54536073 MS.gene88743:intron 25.0%
ATTTGGAAAACCATGTTATG+TGG - chr5.4:54536584-54536603 None:intergenic 30.0%
CAAGACCAAAATCTTCAAAA+AGG + chr5.4:54536536-54536555 MS.gene88743:CDS 30.0%
GAAAGTAACATAGAATTCTG+TGG - chr5.4:54536374-54536393 None:intergenic 30.0%
GAATGATCAACAAAATGCTA+TGG + chr5.4:54536445-54536464 MS.gene88743:CDS 30.0%
GAGTAGGTTATGATATAAGT+TGG + chr5.4:54536044-54536063 MS.gene88743:intron 30.0%
GATGTTTGTAATATAGAAGC+TGG + chr5.4:54535401-54535420 MS.gene88743:CDS 30.0%
TACAAAGGTAATTTAGACCT+AGG + chr5.4:54535833-54535852 MS.gene88743:intron 30.0%
TGATGTGATGAGACAATATA+AGG + chr5.4:54535367-54535386 MS.gene88743:CDS 30.0%
! GAAAGTATAGGAATAAAGGA+TGG + chr5.4:54535806-54535825 MS.gene88743:intron 30.0%
! TTCACCTTTTACCTTAGATT+GGG + chr5.4:54535457-54535476 MS.gene88743:CDS 30.0%
! TTGTGAGCTTAAGAAAGTAT+AGG + chr5.4:54535794-54535813 MS.gene88743:intron 30.0%
!! CAAGACCTTTTTGAAGATTT+TGG - chr5.4:54536544-54536563 None:intergenic 30.0%
AAAGGTAATTTAGACCTAGG+AGG + chr5.4:54535836-54535855 MS.gene88743:intron 35.0%
AAAGTAGCTGAAATGATACC+AGG + chr5.4:54535332-54535351 MS.gene88743:CDS 35.0%
ACAAAACTCCAATTGGTGTA+TGG + chr5.4:54535214-54535233 MS.gene88743:CDS 35.0%
ATAAAGATACACCTGATCGA+TGG + chr5.4:54535306-54535325 MS.gene88743:CDS 35.0%
CATACACACAAAACTCCAAT+TGG + chr5.4:54535207-54535226 MS.gene88743:CDS 35.0%
GTCTTCTTATCCACATAACA+TGG + chr5.4:54536571-54536590 MS.gene88743:CDS 35.0%
TACCTTACAAAAGTCGAGTT+AGG + chr5.4:54535992-54536011 MS.gene88743:intron 35.0%
TATGATGAATTCAGAGGAAG+TGG + chr5.4:54535494-54535513 MS.gene88743:CDS 35.0%
TCATAGGAGTTAAAACCATG+AGG - chr5.4:54536506-54536525 None:intergenic 35.0%
TCTAACACTAGTAGCTCAAT+TGG - chr5.4:54535737-54535756 None:intergenic 35.0%
TCTGGTTATGATGAATTCAG+AGG + chr5.4:54535488-54535507 MS.gene88743:CDS 35.0%
TGAAATGATACCAGGAAAGA+CGG + chr5.4:54535340-54535359 MS.gene88743:CDS 35.0%
TTCATAAGACAACTTTCAGG+AGG + chr5.4:54536245-54536264 MS.gene88743:CDS 35.0%
! AACTCGACTTTTGTAAGGTA+AGG - chr5.4:54535992-54536011 None:intergenic 35.0%
! ACTAGTAGCTCAATTGGTTT+GGG - chr5.4:54535731-54535750 None:intergenic 35.0%
! CTTCACCTTTTACCTTAGAT+TGG + chr5.4:54535456-54535475 MS.gene88743:CDS 35.0%
! TCTAAGGTAAAAGGTGAAGT+AGG - chr5.4:54535455-54535474 None:intergenic 35.0%
! TTCACCCAATCTAAGGTAAA+AGG - chr5.4:54535464-54535483 None:intergenic 35.0%
! TTTAGACTTATCACTAGCCT+AGG - chr5.4:54535945-54535964 None:intergenic 35.0%
!! GTCTTGCATTTTAACCTCAT+AGG - chr5.4:54536522-54536541 None:intergenic 35.0%
!! TCCTGCAGATTATTTCTACT+AGG + chr5.4:54536125-54536144 MS.gene88743:intron 35.0%
!!! AGGTTTTTTAGCTTTCACCT+AGG + chr5.4:54535925-54535944 MS.gene88743:intron 35.0%
!!! TCATGGTTTTAACTCCTATG+AGG + chr5.4:54536505-54536524 MS.gene88743:CDS 35.0%
AAGATAGCATGACCACACAA+TGG + chr5.4:54535237-54535256 MS.gene88743:CDS 40.0%
AATTGATGTTCCTACCTCCT+AGG - chr5.4:54535853-54535872 None:intergenic 40.0%
ACTTGTTCTTCAGAAGACAC+CGG + chr5.4:54536332-54536351 MS.gene88743:CDS 40.0%
AGAACTGTTCACCCAATCTA+AGG - chr5.4:54535471-54535490 None:intergenic 40.0%
CACCTTTGCCATACTTCTTT+AGG - chr5.4:54536151-54536170 None:intergenic 40.0%
CACTAGTAGCTCAATTGGTT+TGG - chr5.4:54535732-54535751 None:intergenic 40.0%
CATAGAATTCTGTGGTGAAG+TGG - chr5.4:54536366-54536385 None:intergenic 40.0%
CTACTAGGCCTAAAGAAGTA+TGG + chr5.4:54536140-54536159 MS.gene88743:CDS 40.0%
GCCTAGTAGAAATAATCTGC+AGG - chr5.4:54536129-54536148 None:intergenic 40.0%
GCTATCTTCCATACACCAAT+TGG - chr5.4:54535225-54535244 None:intergenic 40.0%
GTAATTTAGACCTAGGAGGT+AGG + chr5.4:54535840-54535859 MS.gene88743:intron 40.0%
GTTGTGATGAACGTTGCATT+TGG - chr5.4:54536601-54536620 None:intergenic 40.0%
TGGTGAAGTGGATCTATTAC+CGG - chr5.4:54536354-54536373 None:intergenic 40.0%
TTAGATTGGGTGAACAGTTC+TGG + chr5.4:54535470-54535489 MS.gene88743:CDS 40.0%
TTTCAGGAGGAAAAGACAAG+AGG + chr5.4:54536258-54536277 MS.gene88743:CDS 40.0%
! AGAAGTATGGCAAAGGTGAT+TGG + chr5.4:54536153-54536172 MS.gene88743:CDS 40.0%
! CAAGTTTTCATGGATCCTCA+TGG + chr5.4:54536488-54536507 MS.gene88743:CDS 40.0%
! GACCTAACTCGACTTTTGTA+AGG - chr5.4:54535997-54536016 None:intergenic 40.0%
! TTTTGTGTGTATGATGCAGG+AGG - chr5.4:54535200-54535219 None:intergenic 40.0%
!! GAAGTAGGAGTGTTATAACC+AGG - chr5.4:54535440-54535459 None:intergenic 40.0%
!! GAGTTTTGTGTGTATGATGC+AGG - chr5.4:54535203-54535222 None:intergenic 40.0%
ATGATACCAGGAAAGACGGT+CGG + chr5.4:54535344-54535363 MS.gene88743:CDS 45.0%
TTGTGTTCCTCTTCAGTCCA+TGG - chr5.4:54535572-54535591 None:intergenic 45.0%
TTGTTGCCGTCGAAGTTTGT+TGG - chr5.4:54536412-54536431 None:intergenic 45.0%
TTTAGTTAGAGCTCCTGAGC+AGG + chr5.4:54535529-54535548 MS.gene88743:CDS 45.0%
! AGCTCACGAGCAAGTTTTCA+TGG + chr5.4:54536478-54536497 MS.gene88743:CDS 45.0%
! ATGAAAACTTGCTCGTGAGC+TGG - chr5.4:54536479-54536498 None:intergenic 45.0%
! CGACGGCAACAAATTTTCGT+TGG + chr5.4:54536420-54536439 MS.gene88743:CDS 45.0%
! CGGCAACAAATTTTCGTTGG+AGG + chr5.4:54536423-54536442 MS.gene88743:CDS 45.0%
! GCTACTTTATGCCATCGATC+AGG - chr5.4:54535320-54535339 None:intergenic 45.0%
! GGAGTGTTATAACCAGGAAC+TGG - chr5.4:54535434-54535453 None:intergenic 45.0%
! GGCCTAAAGAAGTATGGCAA+AGG + chr5.4:54536146-54536165 MS.gene88743:CDS 45.0%
! TGGTTTGGGCTAACGGTTAA+AGG - chr5.4:54535717-54535736 None:intergenic 45.0%
!! AAAGGAAGAAAGGAGTGCCA+TGG + chr5.4:54535552-54535571 MS.gene88743:CDS 45.0%
!! GCTCAATTGGTTTGGGCTAA+CGG - chr5.4:54535724-54535743 None:intergenic 45.0%
CCTGAGCAGGAAAGGAAGAA+AGG + chr5.4:54535542-54535561 MS.gene88743:CDS 50.0%
CCTTTCTTCCTTTCCTGCTC+AGG - chr5.4:54535545-54535564 None:intergenic 50.0%
GCAGCACCAACAAACTTCGA+CGG + chr5.4:54536403-54536422 MS.gene88743:CDS 50.0%
TTAGAGCTCCTGAGCAGGAA+AGG + chr5.4:54535534-54535553 MS.gene88743:CDS 50.0%
!! AAGTTTGTTGGTGCTGCTGC+TGG - chr5.4:54536400-54536419 None:intergenic 50.0%
!! CTCTTCTGGAGTCCATTGTG+TGG - chr5.4:54535252-54535271 None:intergenic 50.0%
!! GCATGGCTTGCTACTTGAGT+TGG - chr5.4:54536215-54536234 None:intergenic 50.0%
ACATCACCGACCGTCTTTCC+TGG - chr5.4:54535353-54535372 None:intergenic 55.0%
!! AGGAGTGCCATGGACTGAAG+AGG + chr5.4:54535562-54535581 MS.gene88743:CDS 55.0%
!! GCTGGGTTGATTCCAGTTCC+TGG + chr5.4:54535419-54535438 MS.gene88743:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 54535173 54536637 54535173 ID=MS.gene88743
chr5.4 mRNA 54535173 54536637 54535173 ID=MS.gene88743.t1;Parent=MS.gene88743
chr5.4 exon 54535173 54535591 54535173 ID=MS.gene88743.t1.exon1;Parent=MS.gene88743.t1
chr5.4 CDS 54535173 54535591 54535173 ID=cds.MS.gene88743.t1;Parent=MS.gene88743.t1
chr5.4 exon 54536133 54536637 54536133 ID=MS.gene88743.t1.exon2;Parent=MS.gene88743.t1
chr5.4 CDS 54536133 54536637 54536133 ID=cds.MS.gene88743.t1;Parent=MS.gene88743.t1
Gene Sequence

>MS.gene88743

ATGAAGTGGGAAATGGAAGTTCTTCCTCCTGCATCATACACACAAAACTCCAATTGGTGTATGGAAGATAGCATGACCACACAATGGACTCCAGAAGAGAACAAAATATTTGAAAATGCTCTTGCAGTTCATGATAAAGATACACCTGATCGATGGCATAAAGTAGCTGAAATGATACCAGGAAAGACGGTCGGTGATGTGATGAGACAATATAAGGAATTAGAAGATGATGTTTGTAATATAGAAGCTGGGTTGATTCCAGTTCCTGGTTATAACACTCCTACTTCACCTTTTACCTTAGATTGGGTGAACAGTTCTGGTTATGATGAATTCAGAGGAAGTGGAAAGAGATCTTCTTTAGTTAGAGCTCCTGAGCAGGAAAGGAAGAAAGGAGTGCCATGGACTGAAGAGGAACACAAGTAAATTTTTTACCTTTTGTATTTGTTTTGTTATTATTGTTCTTATTGTTTTAGATAGAAAAAAAAATAGTATATTAGTTGTTATGTTAGTATTTTTTCTTTACTAGGGTTTAAATTCTAAACCTTTAACCGTTAGCCCAAACCAATTGAGCTACTAGTGTTAGATAGATATAGAAGAATGTATGTATGTATGTTTGAAACATTGTGAGCTTAAGAAAGTATAGGAATAAAGGATGGAATTTACAAAGGTAATTTAGACCTAGGAGGTAGGAACATCAATTATTTATAAATATATTTAAAGGGTTGGATATTTTCATTTTTCAGGTTGTAATAAGGTTTTTTAGCTTTCACCTAGGCTAGTGATAAGTCTAAATAAAGCTTATAAAATTTTGACAATCCTTACCTTACAAAAGTCGAGTTAGGTCAAAATCCTCAAATATCATATTAAGTTAGAGTAGGTTATGATATAAGTTGGTTTTGTAGGGCTGATTTAGACTAAAACCTTCTGTTACTATCTTAAGTTAGAGTATATTTCCTGCAGATTATTTCTACTAGGCCTAAAGAAGTATGGCAAAGGTGATTGGAGAAATATTTCGCGCAATTTCGTCATCACAAGAACACCAACTCAAGTAGCAAGCCATGCTCAAAAGTATTTCATAAGACAACTTTCAGGAGGAAAAGACAAGAGGAGAGCAAGCATACATGACATAACAACAGTTAATCTTTCAGAAAAAATTGGAACTTGTTCTTCAGAAGACACCGGTAATAGATCCACTTCACCACAGAATTCTATGTTACTTTCACACCAGCAGCAGCACCAACAAACTTCGACGGCAACAAATTTTCGTTGGAGGAATGATCAACAAAATGCTATGGCTTTGAATCCAGCTCACGAGCAAGTTTTCATGGATCCTCATGGTTTTAACTCCTATGAGGTTAAAATGCAAGACCAAAATCTTCAAAAAGGTCTTGTTCATGAGTCTTCTTATCCACATAACATGGTTTTCCAAATGCAACGTTCATCACAACATTATTCACATGCATAA
Protein sequence

>MS.gene88743.t1

MKWEMEVLPPASYTQNSNWCMEDSMTTQWTPEENKIFENALAVHDKDTPDRWHKVAEMIPGKTVGDVMRQYKELEDDVCNIEAGLIPVPGYNTPTSPFTLDWVNSSGYDEFRGSGKRSSLVRAPEQERKKGVPWTEEEHKLFLLGLKKYGKGDWRNISRNFVITRTPTQVASHAQKYFIRQLSGGKDKRRASIHDITTVNLSEKIGTCSSEDTGNRSTSPQNSMLLSHQQQHQQTSTATNFRWRNDQQNAMALNPAHEQVFMDPHGFNSYEVKMQDQNLQKGLVHESSYPHNMVFQMQRSSQHYSHA