AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89421


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89421.t1 MTR_3g045440 97.398 269 7 0 1 269 1 269 0.0 545
MS.gene89421.t1 MTR_6g086560 63.498 263 95 1 8 269 17 279 3.90e-125 357
MS.gene89421.t1 MTR_1g018320 38.077 260 159 2 7 265 5 263 6.72e-73 224
MS.gene89421.t1 MTR_4g095310 41.224 245 142 2 13 256 9 252 1.12e-70 219
MS.gene89421.t1 MTR_5g016150 38.367 245 149 2 13 256 9 252 2.37e-67 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89421.t1 AT3G24420 50.185 271 132 2 1 268 1 271 2.81e-104 304
MS.gene89421.t1 AT3G03990 38.697 261 159 1 5 265 3 262 1.05e-75 231
MS.gene89421.t1 AT4G37470 42.041 245 140 2 13 256 9 252 3.43e-71 220

Find 54 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAAGCATAGGGCATTATT+TGG 0.168365 3.3:-31924964 MS.gene89421:CDS
AAATAATGCCCTATGCTTAT+TGG 0.181198 3.3:+31924966 None:intergenic
TATTTGGATGAGAACATTAA+AGG 0.265555 3.3:-31924948 MS.gene89421:CDS
TGACATAAAACTATTGTTTC+GGG 0.281340 3.3:+31926222 None:intergenic
ATGACATAAAACTATTGTTT+CGG 0.322926 3.3:+31926221 None:intergenic
TTGGAACAGACCAGTCTATA+TGG 0.354404 3.3:-31926194 MS.gene89421:CDS
TCAACAGAGTCAGCATCATT+TGG 0.359346 3.3:+31925134 None:intergenic
ACAGTCTAGTTCTGTTTGAT+TGG 0.364702 3.3:-31926140 MS.gene89421:CDS
TGATGAGTTAATCACTCTTA+TGG 0.373825 3.3:-31926046 MS.gene89421:CDS
TTTCAGTGACTATAGAGATA+TGG 0.378657 3.3:-31925045 MS.gene89421:CDS
GCTTTCGTATTGAGACTCTA+TGG 0.399369 3.3:+31925190 None:intergenic
ATTTGGATGAGAACATTAAA+GGG 0.399918 3.3:-31924947 MS.gene89421:CDS
TCTGTAATAGCTCCAGAAAA+TGG 0.417553 3.3:+31926117 None:intergenic
CATTAAAGGGGTGTCTACTT+TGG 0.418244 3.3:-31924934 MS.gene89421:CDS
TCATTTGGATCCACTGCAAT+AGG 0.424577 3.3:+31925149 None:intergenic
TAGAGTCTCAATACGAAAGC+TGG 0.436550 3.3:-31925187 MS.gene89421:CDS
CTCATCAGCATAAGGTTCAT+AGG 0.436848 3.3:+31926062 None:intergenic
ACAATCCTCTTAAAGAGCTC+AGG 0.437679 3.3:+31925943 None:intergenic
ATAAATACAGATGACTATAA+AGG 0.458026 3.3:-31925251 MS.gene89421:CDS
CTCAGTTAACTGCACACCTT+AGG 0.466113 3.3:-31924884 MS.gene89421:CDS
CTATAGAGATATGGTTGAAA+AGG 0.466254 3.3:-31925036 MS.gene89421:CDS
CGAGGTGCTGAAGGGTATTG+TGG 0.466637 3.3:-31924856 MS.gene89421:CDS
TGTTTCGGGTCCTGAGCCTA+TGG 0.470244 3.3:+31926236 None:intergenic
CACTCTATGTCTGCTATGAT+AGG 0.473029 3.3:-31925988 MS.gene89421:CDS
TTCTTCTTTGTGCTTCCCCA+AGG 0.483467 3.3:-31925921 MS.gene89421:intron
GTGATTAACTCATCAGCATA+AGG 0.495421 3.3:+31926054 None:intergenic
AATCACTCTTATGGATGAGT+TGG 0.497837 3.3:-31926037 MS.gene89421:CDS
CAACAAAAGTGATACATTTG+AGG 0.498332 3.3:+31926013 None:intergenic
TAGGTTGGTCGAGGTGCTGA+AGG 0.501148 3.3:-31924865 MS.gene89421:CDS
GTGCAGTTAACTGAGGGAAA+TGG 0.509673 3.3:+31924892 None:intergenic
TTGGAGTTTATAGACATGAC+TGG 0.548171 3.3:-31924915 MS.gene89421:CDS
AATGCAAGAACCATAGGCTC+AGG 0.553052 3.3:-31926246 MS.gene89421:CDS
GAAAGCTGGGTTTCAGCATA+CGG 0.556562 3.3:-31925173 MS.gene89421:CDS
TCTTTAAATGCAAGAACCAT+AGG 0.556752 3.3:-31926252 MS.gene89421:CDS
GTGGCAGTTCCAATAAGCAT+AGG 0.560917 3.3:-31924975 MS.gene89421:CDS
ACTTGACTGAATGATTGTGC+AGG 0.563263 3.3:+31925004 None:intergenic
GAGGTGCTGAAGGGTATTGT+GGG 0.563926 3.3:-31924855 MS.gene89421:CDS
AGCATACGGTCCTATTGCAG+TGG 0.564652 3.3:-31925159 MS.gene89421:CDS
TCAGCACCTCGACCAACCTA+AGG 0.574763 3.3:+31924868 None:intergenic
TTGCATTTAAAGATGTTGAG+AGG 0.576785 3.3:+31926262 None:intergenic
AGAGTCTCAATACGAAAGCT+GGG 0.578249 3.3:-31925186 MS.gene89421:CDS
TGGCAGTTCCAATAAGCATA+GGG 0.586920 3.3:-31924974 MS.gene89421:CDS
GTTAACTGCACACCTTAGGT+TGG 0.595927 3.3:-31924880 MS.gene89421:CDS
TTTGGATGAGAACATTAAAG+GGG 0.595928 3.3:-31924946 MS.gene89421:CDS
CTTGACTGAATGATTGTGCA+GGG 0.604202 3.3:+31925005 None:intergenic
TGCACACCTTAGGTTGGTCG+AGG 0.612843 3.3:-31924874 MS.gene89421:CDS
AGGTTGGTCGAGGTGCTGAA+GGG 0.615703 3.3:-31924864 MS.gene89421:CDS
AATACAGATGACTATAAAGG+AGG 0.635005 3.3:-31925248 MS.gene89421:CDS
CATTCAGTCAAGTAATGATG+TGG 0.635222 3.3:-31924994 MS.gene89421:CDS
CTAAGGTGTGCAGTTAACTG+AGG 0.643140 3.3:+31924885 None:intergenic
TAAGGTGTGCAGTTAACTGA+GGG 0.643687 3.3:+31924886 None:intergenic
TGGTGAAGTTGCAATTACCT+TGG 0.664064 3.3:-31925081 MS.gene89421:CDS
AAATGCTTGAAAAGAATGAG+TGG 0.685359 3.3:-31925101 MS.gene89421:CDS
TTGACTGAATGATTGTGCAG+GGG 0.705699 3.3:+31925006 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACTTTTTAACTATGTTAT+TGG - chr3.3:31925587-31925606 MS.gene89421:intron 15.0%
!!! TATATTTTGCATGATTATAA+AGG + chr3.3:31925048-31925067 None:intergenic 15.0%
!! AATTAGTTATTGACAAAACT+CGG + chr3.3:31925626-31925645 None:intergenic 20.0%
!! ACCTATGAATGAATAATTAT+GGG + chr3.3:31925860-31925879 None:intergenic 20.0%
!! ATAAATACAGATGACTATAA+AGG - chr3.3:31925882-31925901 MS.gene89421:intron 20.0%
!! ATGACATAAAACTATTGTTT+CGG + chr3.3:31924915-31924934 None:intergenic 20.0%
!! TACCTATGAATGAATAATTA+TGG + chr3.3:31925861-31925880 None:intergenic 20.0%
!! TGTTAAAACACTTGATTATT+TGG - chr3.3:31925336-31925355 MS.gene89421:intron 20.0%
!!! AAACAATAGTTTTATGTCAT+GGG - chr3.3:31924915-31924934 MS.gene89421:CDS 20.0%
! ATTAGTTATTGACAAAACTC+GGG + chr3.3:31925625-31925644 None:intergenic 25.0%
! ATTTGGATGAGAACATTAAA+GGG - chr3.3:31926186-31926205 MS.gene89421:CDS 25.0%
! GAAATCTCATATTGTGTATT+TGG - chr3.3:31925360-31925379 MS.gene89421:intron 25.0%
! TATTTGGATGAGAACATTAA+AGG - chr3.3:31926185-31926204 MS.gene89421:CDS 25.0%
! TCCCATAATTATTCATTCAT+AGG - chr3.3:31925856-31925875 MS.gene89421:intron 25.0%
! TGACATAAAACTATTGTTTC+GGG + chr3.3:31924914-31924933 None:intergenic 25.0%
!! AAACATTTGATGGTACAATA+TGG - chr3.3:31925806-31925825 MS.gene89421:intron 25.0%
!! AACATTTGATGGTACAATAT+GGG - chr3.3:31925807-31925826 MS.gene89421:intron 25.0%
!! CAATTTTATTCCATATAGAC+TGG + chr3.3:31924952-31924971 None:intergenic 25.0%
!! CTGTAATTTTCAGTTAAAAG+AGG + chr3.3:31924977-31924996 None:intergenic 25.0%
!!! GAAACAATAGTTTTATGTCA+TGG - chr3.3:31924914-31924933 MS.gene89421:CDS 25.0%
AAATAATGCCCTATGCTTAT+TGG + chr3.3:31926170-31926189 None:intergenic 30.0%
AAATGCTTGAAAAGAATGAG+TGG - chr3.3:31926032-31926051 MS.gene89421:CDS 30.0%
AATACAGATGACTATAAAGG+AGG - chr3.3:31925885-31925904 MS.gene89421:intron 30.0%
CAACAAAAGTGATACATTTG+AGG + chr3.3:31925123-31925142 None:intergenic 30.0%
CTATAGAGATATGGTTGAAA+AGG - chr3.3:31926097-31926116 MS.gene89421:CDS 30.0%
GTACCATCAAATGTTTGAAT+TGG + chr3.3:31925802-31925821 None:intergenic 30.0%
TCTTTAAATGCAAGAACCAT+AGG - chr3.3:31924881-31924900 MS.gene89421:CDS 30.0%
TGATGAGTTAATCACTCTTA+TGG - chr3.3:31925087-31925106 MS.gene89421:CDS 30.0%
TTAGTTATTGACAAAACTCG+GGG + chr3.3:31925624-31925643 None:intergenic 30.0%
TTGCATTTAAAGATGTTGAG+AGG + chr3.3:31924874-31924893 None:intergenic 30.0%
TTTCAGTGACTATAGAGATA+TGG - chr3.3:31926088-31926107 MS.gene89421:CDS 30.0%
TTTGGATGAGAACATTAAAG+GGG - chr3.3:31926187-31926206 MS.gene89421:CDS 30.0%
! AATAAGCATAGGGCATTATT+TGG - chr3.3:31926169-31926188 MS.gene89421:CDS 30.0%
! TTTTGTGGAAGGACAAAAAT+AGG + chr3.3:31925733-31925752 None:intergenic 30.0%
!! CTCAAATGTATCACTTTTGT+TGG - chr3.3:31925121-31925140 MS.gene89421:CDS 30.0%
!!! AAAGAGCTCAGGTTTTTTAA+TGG + chr3.3:31925182-31925201 None:intergenic 30.0%
!!! ATAGTTTTATGTCATGGGTT+TGG - chr3.3:31924920-31924939 MS.gene89421:CDS 30.0%
!!! GTTTAGCATTTCTAGTTTTG+TGG + chr3.3:31925748-31925767 None:intergenic 30.0%
AAAAACCTGAGCTCTTTAAG+AGG - chr3.3:31925185-31925204 MS.gene89421:CDS 35.0%
AATCACTCTTATGGATGAGT+TGG - chr3.3:31925096-31925115 MS.gene89421:CDS 35.0%
AGGCCAATTCAAACATTTGA+TGG - chr3.3:31925796-31925815 MS.gene89421:intron 35.0%
CATTCAGTCAAGTAATGATG+TGG - chr3.3:31926139-31926158 MS.gene89421:CDS 35.0%
GAAAAACATAGATGTGAGAG+AGG + chr3.3:31925685-31925704 None:intergenic 35.0%
GTGATTAACTCATCAGCATA+AGG + chr3.3:31925082-31925101 None:intergenic 35.0%
TAGTTATTGACAAAACTCGG+GGG + chr3.3:31925623-31925642 None:intergenic 35.0%
TCTGTAATAGCTCCAGAAAA+TGG + chr3.3:31925019-31925038 None:intergenic 35.0%
TTGGAGTTTATAGACATGAC+TGG - chr3.3:31926218-31926237 MS.gene89421:CDS 35.0%
! ACATCAGAGAGAGTATATGT+AGG + chr3.3:31925252-31925271 None:intergenic 35.0%
! ATTTGATGGTACAATATGGG+TGG - chr3.3:31925810-31925829 MS.gene89421:intron 35.0%
! GTCACTGAAAAACACAGTTT+TGG + chr3.3:31926078-31926097 None:intergenic 35.0%
! TCAAATGTTTGAATTGGCCT+AGG + chr3.3:31925796-31925815 None:intergenic 35.0%
!! ACAGTCTAGTTCTGTTTGAT+TGG - chr3.3:31924993-31925012 MS.gene89421:CDS 35.0%
!!! AGCATTTCTAGTTTTGTGGA+AGG + chr3.3:31925744-31925763 None:intergenic 35.0%
!!! TCACTTTTTTTTCCTAGCCT+AGG - chr3.3:31925776-31925795 MS.gene89421:intron 35.0%
ACAATCCTCTTAAAGAGCTC+AGG + chr3.3:31925193-31925212 None:intergenic 40.0%
ACTTGACTGAATGATTGTGC+AGG + chr3.3:31926132-31926151 None:intergenic 40.0%
AGAGTCTCAATACGAAAGCT+GGG - chr3.3:31925947-31925966 MS.gene89421:CDS 40.0%
CACTCTATGTCTGCTATGAT+AGG - chr3.3:31925145-31925164 MS.gene89421:CDS 40.0%
CATGTGTTACCGACATATGT+CGG - chr3.3:31925491-31925510 MS.gene89421:intron 40.0%
CTCATCAGCATAAGGTTCAT+AGG + chr3.3:31925074-31925093 None:intergenic 40.0%
CTTGACTGAATGATTGTGCA+GGG + chr3.3:31926131-31926150 None:intergenic 40.0%
GCTTTCGTATTGAGACTCTA+TGG + chr3.3:31925946-31925965 None:intergenic 40.0%
GTGCACTTCTTTATGTGTTG+TGG + chr3.3:31925711-31925730 None:intergenic 40.0%
TAAGGTGTGCAGTTAACTGA+GGG + chr3.3:31926250-31926269 None:intergenic 40.0%
TAGAGTCTCAATACGAAAGC+TGG - chr3.3:31925946-31925965 MS.gene89421:CDS 40.0%
TAGTGTCAACGTGTTAGTGA+CGG + chr3.3:31925428-31925447 None:intergenic 40.0%
TCAACAGAGTCAGCATCATT+TGG + chr3.3:31926002-31926021 None:intergenic 40.0%
TCATTTGGATCCACTGCAAT+AGG + chr3.3:31925987-31926006 None:intergenic 40.0%
TGGCAGTTCCAATAAGCATA+GGG - chr3.3:31926159-31926178 MS.gene89421:CDS 40.0%
TGGTGAAGTTGCAATTACCT+TGG - chr3.3:31926052-31926071 MS.gene89421:CDS 40.0%
TTGACTGAATGATTGTGCAG+GGG + chr3.3:31926130-31926149 None:intergenic 40.0%
TTGGAACAGACCAGTCTATA+TGG - chr3.3:31924939-31924958 MS.gene89421:CDS 40.0%
! ACACTTTCCATCAGAAGTGT+CGG - chr3.3:31925523-31925542 MS.gene89421:intron 40.0%
! GAGAGAGGCTACTTTAAAGA+TGG + chr3.3:31925670-31925689 None:intergenic 40.0%
!! AGGTAAAGATGAGAGTACCT+TGG + chr3.3:31925232-31925251 None:intergenic 40.0%
!! CATTAAAGGGGTGTCTACTT+TGG - chr3.3:31926199-31926218 MS.gene89421:CDS 40.0%
!! CTGTTTGATTGGCCATTTTC+TGG - chr3.3:31925004-31925023 MS.gene89421:CDS 40.0%
!! GAAAAACACAGTTTTGGCCA+AGG + chr3.3:31926072-31926091 None:intergenic 40.0%
!! GGTAAAGATGAGAGTACCTT+GGG + chr3.3:31925231-31925250 None:intergenic 40.0%
!! GTAAAGATGAGAGTACCTTG+GGG + chr3.3:31925230-31925249 None:intergenic 40.0%
!!! GAGCTCAGGTTTTTTAATGG+AGG + chr3.3:31925179-31925198 None:intergenic 40.0%
!!! GATGTTGAGAGGCTCTTTTT+TGG + chr3.3:31924863-31924882 None:intergenic 40.0%
AATGCAAGAACCATAGGCTC+AGG - chr3.3:31924887-31924906 MS.gene89421:CDS 45.0%
CTAAGGTGTGCAGTTAACTG+AGG + chr3.3:31926251-31926270 None:intergenic 45.0%
CTCAGTTAACTGCACACCTT+AGG - chr3.3:31926249-31926268 MS.gene89421:CDS 45.0%
GAAAGCTGGGTTTCAGCATA+CGG - chr3.3:31925960-31925979 MS.gene89421:CDS 45.0%
GTCTGATGTCCGACATATGT+CGG + chr3.3:31925503-31925522 None:intergenic 45.0%
GTGCAGTTAACTGAGGGAAA+TGG + chr3.3:31926244-31926263 None:intergenic 45.0%
GTGGCAGTTCCAATAAGCAT+AGG - chr3.3:31926158-31926177 MS.gene89421:CDS 45.0%
GTTAACTGCACACCTTAGGT+TGG - chr3.3:31926253-31926272 MS.gene89421:CDS 45.0%
TTCTTCTTTGTGCTTCCCCA+AGG - chr3.3:31925212-31925231 MS.gene89421:CDS 45.0%
! TGTTTGAATTGGCCTAGGCT+AGG + chr3.3:31925791-31925810 None:intergenic 45.0%
!! GTGTCGGTGCTACAAAGATT+TGG - chr3.3:31925539-31925558 MS.gene89421:intron 45.0%
AGCATACGGTCCTATTGCAG+TGG - chr3.3:31925974-31925993 MS.gene89421:CDS 50.0%
!! GAGGTGCTGAAGGGTATTGT+GGG - chr3.3:31926278-31926297 MS.gene89421:CDS 50.0%
!! TGTAGCACCGACACTTCTGA+TGG + chr3.3:31925533-31925552 None:intergenic 50.0%
TCAGCACCTCGACCAACCTA+AGG + chr3.3:31926268-31926287 None:intergenic 55.0%
! TGTTTCGGGTCCTGAGCCTA+TGG + chr3.3:31924900-31924919 None:intergenic 55.0%
!! AGGTTGGTCGAGGTGCTGAA+GGG - chr3.3:31926269-31926288 MS.gene89421:CDS 55.0%
!! CGAGGTGCTGAAGGGTATTG+TGG - chr3.3:31926277-31926296 MS.gene89421:CDS 55.0%
!! TAGGTTGGTCGAGGTGCTGA+AGG - chr3.3:31926268-31926287 MS.gene89421:CDS 55.0%
!! TGCACACCTTAGGTTGGTCG+AGG - chr3.3:31926259-31926278 MS.gene89421:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 31924851 31926304 31924851 ID=MS.gene89421
chr3.3 mRNA 31924851 31926304 31924851 ID=MS.gene89421.t1;Parent=MS.gene89421
chr3.3 exon 31925922 31926304 31925922 ID=MS.gene89421.t1.exon1;Parent=MS.gene89421.t1
chr3.3 CDS 31925922 31926304 31925922 ID=cds.MS.gene89421.t1;Parent=MS.gene89421.t1
chr3.3 exon 31924851 31925277 31924851 ID=MS.gene89421.t1.exon2;Parent=MS.gene89421.t1
chr3.3 CDS 31924851 31925277 31924851 ID=cds.MS.gene89421.t1;Parent=MS.gene89421.t1
Gene Sequence

>MS.gene89421

ATGGTGACACCAAAAAAGAGCCTCTCAACATCTTTAAATGCAAGAACCATAGGCTCAGGACCCGAAACAATAGTTTTATGTCATGGGTTTGGAACAGACCAGTCTATATGGAATAAAATTGTTCCTCTTTTAACTGAAAATTACAGTCTAGTTCTGTTTGATTGGCCATTTTCTGGAGCTATTACAGATAAAAGCCTTTATAATCATGCAAAATATAATTCCTATGAACCTTATGCTGATGAGTTAATCACTCTTATGGATGAGTTGGACCTCAAATGTATCACTTTTGTTGGACACTCTATGTCTGCTATGATAGGTTGCGTTGCCTCCATTAAAAAACCTGAGCTCTTTAAGAGGATTGTTCTTCTTTGTGCTTCCCCAAGGTACTCTCATCTTTACCTACATATACTCTCTCTGATGTTGTCTTTTTTTTTTATTCTCTTTTTATGAAAATACTAGAAGTAACACAATATTTGAGATTCTGTTGTTAAAACACTTGATTATTTGGTGAAATCTCATATTGTGTATTTGGATCTATGAAGTATCGTCACAAACACTAATATCATATAAGACACCGTCACTAACACGTTGACACTAGTAAAAATATGAAGAAAAAAAATGAATTAATTGAACGTAATCACATGTGTTACCGACATATGTCGGACATCAGACACACTTTCCATCAGAAGTGTCGGTGCTACAAAGATTTGGATAAATATTTGAAAGTAATTTAAAGTAACTTTTTAACTATGTTATTGGTGCAAAGTCTCCCCCGAGTTTTGTCAATAACTAATTTCTGTCGAGAAAATTCACTAACCATCTTTAAAGTAGCCTCTCTCACATCTATGTTTTTCTATCCACAACACATAAAGAAGTGCACCTATTTTTGTCCTTCCACAAAACTAGAAATGCTAAACTCGATAAATCACTTTTTTTTCCTAGCCTAGGCCAATTCAAACATTTGATGGTACAATATGGGTGGAATCAAATAGTCTTGCATTAACATCCCATAATTATTCATTCATAGGTATATAAATACAGATGACTATAAAGGAGGCTTTGAGAGATCAGATATTGAGAACTTAATATCAACCATAGAGTCTCAATACGAAAGCTGGGTTTCAGCATACGGTCCTATTGCAGTGGATCCAAATGATGCTGACTCTGTTGACAAATTTCAAAAATGCTTGAAAAGAATGAGTGGTGAAGTTGCAATTACCTTGGCCAAAACTGTGTTTTTCAGTGACTATAGAGATATGGTTGAAAAGGTAGTGATCCCCTGCACAATCATTCAGTCAAGTAATGATGTGGCAGTTCCAATAAGCATAGGGCATTATTTGGATGAGAACATTAAAGGGGTGTCTACTTTGGAGTTTATAGACATGACTGGCCATTTCCCTCAGTTAACTGCACACCTTAGGTTGGTCGAGGTGCTGAAGGGTATTGTGGGTTAG

Protein sequence

>MS.gene89421.t1

MVTPKKSLSTSLNARTIGSGPETIVLCHGFGTDQSIWNKIVPLLTENYSLVLFDWPFSGAITDKSLYNHAKYNSYEPYADELITLMDELDLKCITFVGHSMSAMIGCVASIKKPELFKRIVLLCASPRYINTDDYKGGFERSDIENLISTIESQYESWVSAYGPIAVDPNDADSVDKFQKCLKRMSGEVAITLAKTVFFSDYRDMVEKVVIPCTIIQSSNDVAVPISIGHYLDENIKGVSTLEFIDMTGHFPQLTAHLRLVEVLKGIVG