Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89641.t1 | RHN42004.1 | 96.9 | 193 | 6 | 0 | 1 | 193 | 1 | 193 | 1.60E-104 | 388.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89641.t1 | A0A396GR79 | 96.9 | 193 | 6 | 0 | 1 | 193 | 1 | 193 | 1.2e-104 | 388.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene89641 | 0.809677 | 1.68E-50 | -1.69E-46 |
| MS.gene050808 | MS.gene89641 | 0.802092 | 6.61E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene89641 | 0.805321 | 1.41E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene89641 | 0.824839 | 6.49E-54 | -1.69E-46 |
| MS.gene051265 | MS.gene89641 | 0.829184 | 5.93E-55 | -1.69E-46 |
| MS.gene053630 | MS.gene89641 | 0.802718 | 4.91E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene89641 | 0.80473 | 1.88E-49 | -1.69E-46 |
| MS.gene055608 | MS.gene89641 | 0.840689 | 7.47E-58 | -1.69E-46 |
| MS.gene055818 | MS.gene89641 | 0.805388 | 1.37E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene89641 | 0.805474 | 1.31E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene89641 | 0.804059 | 2.59E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89641.t1 | MTR_8g076160 | 96.373 | 193 | 7 | 0 | 1 | 193 | 1 | 193 | 2.53e-140 | 389 |
| MS.gene89641.t1 | MTR_5g021610 | 76.923 | 195 | 42 | 3 | 1 | 193 | 14 | 207 | 8.49e-108 | 307 |
| MS.gene89641.t1 | MTR_2g005880 | 63.087 | 149 | 49 | 2 | 12 | 160 | 2 | 144 | 1.91e-64 | 197 |
| MS.gene89641.t1 | MTR_4g114080 | 57.639 | 144 | 57 | 1 | 12 | 155 | 4 | 143 | 2.32e-56 | 176 |
| MS.gene89641.t1 | MTR_4g011020 | 53.691 | 149 | 62 | 2 | 12 | 157 | 8 | 152 | 3.47e-55 | 173 |
| MS.gene89641.t1 | MTR_0003s0630 | 47.183 | 142 | 73 | 2 | 14 | 154 | 35 | 175 | 1.37e-47 | 154 |
| MS.gene89641.t1 | MTR_2g103490 | 49.333 | 150 | 68 | 3 | 12 | 157 | 8 | 153 | 4.03e-47 | 152 |
| MS.gene89641.t1 | MTR_2g103460 | 48.667 | 150 | 69 | 3 | 12 | 157 | 8 | 153 | 1.68e-45 | 148 |
| MS.gene89641.t1 | MTR_1g115170 | 44.444 | 135 | 66 | 3 | 30 | 161 | 3 | 131 | 2.39e-36 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89641.t1 | AT1G15380 | 56.376 | 149 | 58 | 2 | 12 | 157 | 8 | 152 | 1.78e-57 | 179 |
| MS.gene89641.t1 | AT1G15380 | 56.376 | 149 | 58 | 2 | 12 | 157 | 8 | 152 | 1.78e-57 | 179 |
| MS.gene89641.t1 | AT1G80160 | 51.678 | 149 | 65 | 2 | 12 | 157 | 8 | 152 | 1.29e-54 | 171 |
| MS.gene89641.t1 | AT1G80160 | 51.678 | 149 | 65 | 2 | 12 | 157 | 49 | 193 | 3.84e-54 | 171 |
| MS.gene89641.t1 | AT2G28420 | 49.306 | 144 | 69 | 2 | 14 | 156 | 17 | 157 | 1.32e-48 | 156 |
| MS.gene89641.t1 | AT1G80160 | 45.217 | 115 | 56 | 2 | 57 | 168 | 27 | 137 | 1.61e-31 | 111 |
Find 58 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCAAATTCATCATAATT+TGG | 0.184282 | 8.3:-27724418 | None:intergenic |
| TTGTGTAGGTTGTATAATTA+TGG | 0.227214 | 8.3:+27724370 | MS.gene89641:intron |
| ATGGTTGTCCTTGGGATTAA+TGG | 0.258018 | 8.3:-27724461 | None:intergenic |
| TAATTACTTGGAATGATATA+TGG | 0.268558 | 8.3:-27724480 | None:intergenic |
| TATGAAGAAATCTTGGGTTT+TGG | 0.309204 | 8.3:+27724200 | MS.gene89641:CDS |
| TGTGTAGGTTGTATAATTAT+GGG | 0.310343 | 8.3:+27724371 | MS.gene89641:intron |
| CAACACTGATCTGCATAATA+TGG | 0.311667 | 8.3:-27724162 | None:intergenic |
| TTCTAACAAGTGTGGATTCA+TGG | 0.313847 | 8.3:+27724857 | MS.gene89641:CDS |
| GCAGAAGAAATGGGAACTAT+TGG | 0.335608 | 8.3:-27724789 | None:intergenic |
| ATGGGTCGTGATTCACTCAT+AGG | 0.351235 | 8.3:-27724442 | None:intergenic |
| GAAAATGAGGTTGGAAGAAA+TGG | 0.352191 | 8.3:+27724656 | MS.gene89641:CDS |
| CCTTCCTCTTTCAAATTCAA+TGG | 0.370841 | 8.3:+27724236 | MS.gene89641:CDS |
| GGAGCAGGAAGCAGAAGAAA+TGG | 0.375124 | 8.3:-27724799 | None:intergenic |
| AAAATGGGGATTGAGGAAAT+TGG | 0.400288 | 8.3:+27724095 | None:intergenic |
| TGGTTGTCCTTGGGATTAAT+GGG | 0.407514 | 8.3:-27724460 | None:intergenic |
| AGGTTGTATAATTATGGGTT+TGG | 0.407784 | 8.3:+27724376 | MS.gene89641:intron |
| CAGAAGAAATGGGAACTATT+GGG | 0.429676 | 8.3:-27724788 | None:intergenic |
| AGTTCTATCATGTATCCATC+AGG | 0.435736 | 8.3:-27724747 | None:intergenic |
| GATGAAGTATGTTACGGCTT+TGG | 0.445021 | 8.3:+27724680 | MS.gene89641:CDS |
| AAACAGTCTTCTTAAAGCTC+TGG | 0.447084 | 8.3:-27724836 | None:intergenic |
| GAATGATATATGGTTGTCCT+TGG | 0.451197 | 8.3:-27724470 | None:intergenic |
| AAAATGAGGTTGGAAGAAAT+GGG | 0.458153 | 8.3:+27724657 | MS.gene89641:CDS |
| GCTCTGGCCTCGTGACTTGA+AGG | 0.458217 | 8.3:-27724820 | None:intergenic |
| TTGTGCAGTGTACAGATGTT+GGG | 0.460233 | 8.3:+27724628 | MS.gene89641:intron |
| CATAATTTGGATTCTCAAGC+AGG | 0.462581 | 8.3:-27724405 | None:intergenic |
| TGCTCCATTGAATTTGAAAG+AGG | 0.466642 | 8.3:-27724240 | None:intergenic |
| CACATGATTCAGTGAGAGAA+GGG | 0.472092 | 8.3:-27724138 | None:intergenic |
| TTTGTGCAGTGTACAGATGT+TGG | 0.472216 | 8.3:+27724627 | MS.gene89641:intron |
| GTATGTTACGGCTTTGGTAG+AGG | 0.491758 | 8.3:+27724686 | MS.gene89641:CDS |
| TGAAGGAATCAAGGTGGAGC+AGG | 0.491848 | 8.3:+27724710 | MS.gene89641:CDS |
| TGGGTCGTGATTCACTCATA+GGG | 0.496984 | 8.3:-27724441 | None:intergenic |
| GCCTCGTGACTTGAAGGAGC+AGG | 0.509012 | 8.3:-27724814 | None:intergenic |
| GAGCAGGAAGCAGAAGAAAT+GGG | 0.511553 | 8.3:-27724798 | None:intergenic |
| AATGGGGATGAAGTATGTTA+CGG | 0.515973 | 8.3:+27724674 | MS.gene89641:CDS |
| TCATGTATCCATCAGGGTCA+TGG | 0.518219 | 8.3:-27724740 | None:intergenic |
| CCATTGAATTTGAAAGAGGA+AGG | 0.520942 | 8.3:-27724236 | None:intergenic |
| AATGATATATGGTTGTCCTT+GGG | 0.521545 | 8.3:-27724469 | None:intergenic |
| ACGGCTTTGGTAGAGGATGA+AGG | 0.527027 | 8.3:+27724693 | MS.gene89641:CDS |
| CTTTCAAATTCAATGGAGCA+TGG | 0.540814 | 8.3:+27724243 | MS.gene89641:CDS |
| TTCAGTGAGAGAAGGGGTAG+TGG | 0.543925 | 8.3:-27724131 | None:intergenic |
| TGGGCTAGTGAAAATGAGGT+TGG | 0.545299 | 8.3:+27724647 | MS.gene89641:CDS |
| GGTAGAGGATGAAGGAATCA+AGG | 0.549754 | 8.3:+27724701 | MS.gene89641:CDS |
| CATATTATGCAGATCAGTGT+TGG | 0.552916 | 8.3:+27724163 | MS.gene89641:CDS |
| GTGTTCTTCCATGACCCTGA+TGG | 0.553401 | 8.3:+27724732 | MS.gene89641:CDS |
| ATATTGGAAAATGGGGATTG+AGG | 0.566391 | 8.3:+27724088 | None:intergenic |
| AAGACTGTTTCTAACAAGTG+TGG | 0.583686 | 8.3:+27724849 | MS.gene89641:CDS |
| ACACATGATTCAGTGAGAGA+AGG | 0.601179 | 8.3:-27724139 | None:intergenic |
| TCCTGCTCCTTCAAGTCACG+AGG | 0.622464 | 8.3:+27724813 | MS.gene89641:CDS |
| GTTCTATCATGTATCCATCA+GGG | 0.630489 | 8.3:-27724746 | None:intergenic |
| CATTGAATTTGAAAGAGGAA+GGG | 0.632398 | 8.3:-27724235 | None:intergenic |
| TATGATGCGAAGCTTGAGCA+AGG | 0.642219 | 8.3:+27724887 | MS.gene89641:CDS |
| ATCACGACCCATTAATCCCA+AGG | 0.645670 | 8.3:+27724453 | MS.gene89641:CDS |
| ATGTTGGGCTAGTGAAAATG+AGG | 0.654745 | 8.3:+27724643 | MS.gene89641:CDS |
| GGGTCGTGATTCACTCATAG+GGG | 0.689430 | 8.3:-27724440 | None:intergenic |
| AAATGAGGTTGGAAGAAATG+GGG | 0.690532 | 8.3:+27724658 | MS.gene89641:CDS |
| ACATGATTCAGTGAGAGAAG+GGG | 0.696257 | 8.3:-27724137 | None:intergenic |
| GATCAGTGTTGGACTCTGTG+AGG | 0.702787 | 8.3:+27724174 | MS.gene89641:CDS |
| AGAGGATGAAGGAATCAAGG+TGG | 0.720837 | 8.3:+27724704 | MS.gene89641:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATTTCATAAAGAAAA+TGG | - | chr8.3:27724296-27724315 | None:intergenic | 10.0% |
| !! | TATTTGTTCAAACAAAATAA+TGG | - | chr8.3:27724321-27724340 | None:intergenic | 15.0% |
| !! | TAATTACTTGGAATGATATA+TGG | - | chr8.3:27724483-27724502 | None:intergenic | 20.0% |
| !!! | TTTATTGTTTTGTTTTGTGT+AGG | + | chr8.3:27724356-27724375 | MS.gene89641:intron | 20.0% |
| ! | GCATCAAATTCATCATAATT+TGG | - | chr8.3:27724421-27724440 | None:intergenic | 25.0% |
| ! | TGTGTAGGTTGTATAATTAT+GGG | + | chr8.3:27724371-27724390 | MS.gene89641:intron | 25.0% |
| ! | TTGTGTAGGTTGTATAATTA+TGG | + | chr8.3:27724370-27724389 | MS.gene89641:intron | 25.0% |
| !!! | AGGTTTTATGAAGAAATCTT+GGG | + | chr8.3:27724194-27724213 | MS.gene89641:CDS | 25.0% |
| !!! | TTACTGAAAGTGTTTCAATT+TGG | + | chr8.3:27724603-27724622 | MS.gene89641:intron | 25.0% |
| AAAATGAGGTTGGAAGAAAT+GGG | + | chr8.3:27724657-27724676 | MS.gene89641:CDS | 30.0% | |
| AATGATATATGGTTGTCCTT+GGG | - | chr8.3:27724472-27724491 | None:intergenic | 30.0% | |
| AGGTTGTATAATTATGGGTT+TGG | + | chr8.3:27724376-27724395 | MS.gene89641:intron | 30.0% | |
| CATTGAATTTGAAAGAGGAA+GGG | - | chr8.3:27724238-27724257 | None:intergenic | 30.0% | |
| GGTAATGAAGCAAAGTATAT+AGG | - | chr8.3:27724275-27724294 | None:intergenic | 30.0% | |
| ! | TATGAAGAAATCTTGGGTTT+TGG | + | chr8.3:27724200-27724219 | MS.gene89641:CDS | 30.0% |
| !! | GAGGTTTTATGAAGAAATCT+TGG | + | chr8.3:27724193-27724212 | MS.gene89641:CDS | 30.0% |
| AAACAGTCTTCTTAAAGCTC+TGG | - | chr8.3:27724839-27724858 | None:intergenic | 35.0% | |
| AAATGAGGTTGGAAGAAATG+GGG | + | chr8.3:27724658-27724677 | MS.gene89641:CDS | 35.0% | |
| AATGGGGATGAAGTATGTTA+CGG | + | chr8.3:27724674-27724693 | MS.gene89641:CDS | 35.0% | |
| ACTAGAAATTCAGAATGCAC+AGG | - | chr8.3:27724507-27724526 | None:intergenic | 35.0% | |
| AGTTCTATCATGTATCCATC+AGG | - | chr8.3:27724750-27724769 | None:intergenic | 35.0% | |
| CAACACTGATCTGCATAATA+TGG | - | chr8.3:27724165-27724184 | None:intergenic | 35.0% | |
| CAGAAGAAATGGGAACTATT+GGG | - | chr8.3:27724791-27724810 | None:intergenic | 35.0% | |
| CATAATTTGGATTCTCAAGC+AGG | - | chr8.3:27724408-27724427 | None:intergenic | 35.0% | |
| CATATTATGCAGATCAGTGT+TGG | + | chr8.3:27724163-27724182 | MS.gene89641:CDS | 35.0% | |
| CCATTGAATTTGAAAGAGGA+AGG | - | chr8.3:27724239-27724258 | None:intergenic | 35.0% | |
| CCTTCCTCTTTCAAATTCAA+TGG | + | chr8.3:27724236-27724255 | MS.gene89641:CDS | 35.0% | |
| CTTTCAAATTCAATGGAGCA+TGG | + | chr8.3:27724243-27724262 | MS.gene89641:CDS | 35.0% | |
| GAAAATGAGGTTGGAAGAAA+TGG | + | chr8.3:27724656-27724675 | MS.gene89641:CDS | 35.0% | |
| GAATGATATATGGTTGTCCT+TGG | - | chr8.3:27724473-27724492 | None:intergenic | 35.0% | |
| GAATGCACAGGTTAATTACT+TGG | - | chr8.3:27724495-27724514 | None:intergenic | 35.0% | |
| GTTCTATCATGTATCCATCA+GGG | - | chr8.3:27724749-27724768 | None:intergenic | 35.0% | |
| TGCTCCATTGAATTTGAAAG+AGG | - | chr8.3:27724243-27724262 | None:intergenic | 35.0% | |
| TTCTAACAAGTGTGGATTCA+TGG | + | chr8.3:27724857-27724876 | MS.gene89641:CDS | 35.0% | |
| !! | AAGACTGTTTCTAACAAGTG+TGG | + | chr8.3:27724849-27724868 | MS.gene89641:CDS | 35.0% |
| ACACATGATTCAGTGAGAGA+AGG | - | chr8.3:27724142-27724161 | None:intergenic | 40.0% | |
| ACATGATTCAGTGAGAGAAG+GGG | - | chr8.3:27724140-27724159 | None:intergenic | 40.0% | |
| ATGGTTGTCCTTGGGATTAA+TGG | - | chr8.3:27724464-27724483 | None:intergenic | 40.0% | |
| CACATGATTCAGTGAGAGAA+GGG | - | chr8.3:27724141-27724160 | None:intergenic | 40.0% | |
| GATGAAGTATGTTACGGCTT+TGG | + | chr8.3:27724680-27724699 | MS.gene89641:CDS | 40.0% | |
| GCAGAAGAAATGGGAACTAT+TGG | - | chr8.3:27724792-27724811 | None:intergenic | 40.0% | |
| TGGTTGTCCTTGGGATTAAT+GGG | - | chr8.3:27724463-27724482 | None:intergenic | 40.0% | |
| TTGTGCAGTGTACAGATGTT+GGG | + | chr8.3:27724628-27724647 | MS.gene89641:intron | 40.0% | |
| TTTGTGCAGTGTACAGATGT+TGG | + | chr8.3:27724627-27724646 | MS.gene89641:intron | 40.0% | |
| !! | ATGTTGGGCTAGTGAAAATG+AGG | + | chr8.3:27724643-27724662 | MS.gene89641:CDS | 40.0% |
| AGAGGATGAAGGAATCAAGG+TGG | + | chr8.3:27724704-27724723 | MS.gene89641:CDS | 45.0% | |
| ATCACGACCCATTAATCCCA+AGG | + | chr8.3:27724453-27724472 | MS.gene89641:CDS | 45.0% | |
| ATGGGTCGTGATTCACTCAT+AGG | - | chr8.3:27724445-27724464 | None:intergenic | 45.0% | |
| GAGCAGGAAGCAGAAGAAAT+GGG | - | chr8.3:27724801-27724820 | None:intergenic | 45.0% | |
| GGTAGAGGATGAAGGAATCA+AGG | + | chr8.3:27724701-27724720 | MS.gene89641:CDS | 45.0% | |
| TATGATGCGAAGCTTGAGCA+AGG | + | chr8.3:27724887-27724906 | MS.gene89641:CDS | 45.0% | |
| TCATGTATCCATCAGGGTCA+TGG | - | chr8.3:27724743-27724762 | None:intergenic | 45.0% | |
| TGGGTCGTGATTCACTCATA+GGG | - | chr8.3:27724444-27724463 | None:intergenic | 45.0% | |
| ! | GTATGTTACGGCTTTGGTAG+AGG | + | chr8.3:27724686-27724705 | MS.gene89641:CDS | 45.0% |
| !! | TGGGCTAGTGAAAATGAGGT+TGG | + | chr8.3:27724647-27724666 | MS.gene89641:CDS | 45.0% |
| GGAGCAGGAAGCAGAAGAAA+TGG | - | chr8.3:27724802-27724821 | None:intergenic | 50.0% | |
| GGGTCGTGATTCACTCATAG+GGG | - | chr8.3:27724443-27724462 | None:intergenic | 50.0% | |
| GTGTTCTTCCATGACCCTGA+TGG | + | chr8.3:27724732-27724751 | MS.gene89641:CDS | 50.0% | |
| TGAAGGAATCAAGGTGGAGC+AGG | + | chr8.3:27724710-27724729 | MS.gene89641:CDS | 50.0% | |
| TTCAGTGAGAGAAGGGGTAG+TGG | - | chr8.3:27724134-27724153 | None:intergenic | 50.0% | |
| ! | ACGGCTTTGGTAGAGGATGA+AGG | + | chr8.3:27724693-27724712 | MS.gene89641:CDS | 50.0% |
| ! | GATCAGTGTTGGACTCTGTG+AGG | + | chr8.3:27724174-27724193 | MS.gene89641:CDS | 50.0% |
| TCCTGCTCCTTCAAGTCACG+AGG | + | chr8.3:27724813-27724832 | MS.gene89641:CDS | 55.0% | |
| GCCTCGTGACTTGAAGGAGC+AGG | - | chr8.3:27724817-27724836 | None:intergenic | 60.0% | |
| GCTCTGGCCTCGTGACTTGA+AGG | - | chr8.3:27724823-27724842 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 27724098 | 27724932 | 27724098 | ID=MS.gene89641 |
| chr8.3 | mRNA | 27724098 | 27724932 | 27724098 | ID=MS.gene89641.t1;Parent=MS.gene89641 |
| chr8.3 | exon | 27724098 | 27724264 | 27724098 | ID=MS.gene89641.t1.exon1;Parent=MS.gene89641.t1 |
| chr8.3 | CDS | 27724098 | 27724264 | 27724098 | ID=cds.MS.gene89641.t1;Parent=MS.gene89641.t1 |
| chr8.3 | exon | 27724378 | 27724495 | 27724378 | ID=MS.gene89641.t1.exon2;Parent=MS.gene89641.t1 |
| chr8.3 | CDS | 27724378 | 27724495 | 27724378 | ID=cds.MS.gene89641.t1;Parent=MS.gene89641.t1 |
| chr8.3 | exon | 27724636 | 27724932 | 27724636 | ID=MS.gene89641.t1.exon3;Parent=MS.gene89641.t1 |
| chr8.3 | CDS | 27724636 | 27724932 | 27724636 | ID=cds.MS.gene89641.t1;Parent=MS.gene89641.t1 |
| Gene Sequence |
| Protein sequence |
