Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89778.t1 | XP_003594552.1 | 97.2 | 71 | 2 | 0 | 37 | 107 | 71 | 141 | 2.80E-29 | 137.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89778.t1 | Q9FKV5 | 74.6 | 71 | 18 | 0 | 37 | 107 | 64 | 134 | 1.8e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89778.t1 | G7IKX2 | 97.2 | 71 | 2 | 0 | 37 | 107 | 71 | 141 | 2.0e-29 | 137.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051710 | MS.gene89778 | 0.808087 | 3.68E-50 | -1.69E-46 |
| MS.gene055056 | MS.gene89778 | 0.802011 | 6.87E-49 | -1.69E-46 |
| MS.gene055058 | MS.gene89778 | 0.803354 | 3.63E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene89778 | 0.823429 | 1.39E-53 | -1.69E-46 |
| MS.gene059276 | MS.gene89778 | 0.811248 | 7.70E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89778.t1 | MTR_2g030510 | 97.183 | 71 | 2 | 0 | 37 | 107 | 71 | 141 | 1.05e-42 | 147 |
| MS.gene89778.t1 | MTR_8g075260 | 72.603 | 73 | 20 | 0 | 35 | 107 | 54 | 126 | 4.14e-30 | 111 |
| MS.gene89778.t1 | MTR_7g059010 | 71.831 | 71 | 20 | 0 | 37 | 107 | 39 | 109 | 5.06e-30 | 108 |
| MS.gene89778.t1 | MTR_7g058930 | 71.831 | 71 | 20 | 0 | 37 | 107 | 44 | 114 | 5.73e-30 | 108 |
| MS.gene89778.t1 | MTR_7g059010 | 71.831 | 71 | 20 | 0 | 37 | 107 | 56 | 126 | 3.88e-29 | 108 |
| MS.gene89778.t1 | MTR_3g005410 | 100.000 | 37 | 0 | 0 | 1 | 37 | 46 | 82 | 1.41e-18 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89778.t1 | AT5G44330 | 74.648 | 71 | 18 | 0 | 37 | 107 | 64 | 134 | 1.75e-32 | 118 |
| MS.gene89778.t1 | AT4G20900 | 74.648 | 71 | 18 | 0 | 37 | 107 | 71 | 141 | 1.93e-32 | 117 |
| MS.gene89778.t1 | AT4G20900 | 74.648 | 71 | 18 | 0 | 37 | 107 | 71 | 141 | 2.47e-32 | 117 |
| MS.gene89778.t1 | AT3G51280 | 69.863 | 73 | 22 | 0 | 35 | 107 | 48 | 120 | 1.09e-28 | 107 |
| MS.gene89778.t1 | AT5G48850 | 66.197 | 71 | 24 | 0 | 37 | 107 | 47 | 117 | 1.07e-27 | 103 |
| MS.gene89778.t1 | AT1G04770 | 64.789 | 71 | 25 | 0 | 37 | 107 | 40 | 110 | 8.64e-26 | 97.8 |
Find 26 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGAACTCTATACGGTATA+AGG | 0.219424 | 4.4:+83635311 | MS.gene89778:CDS |
| TTGTTGACTGGAACAGAATC+AGG | 0.358714 | 4.4:-83634670 | None:intergenic |
| CATCTTGGTTGAACTCTATA+CGG | 0.371370 | 4.4:+83635304 | MS.gene89778:CDS |
| CGAGAGTGCCTTGAAGGATA+TGG | 0.372126 | 4.4:+83635181 | MS.gene89778:CDS |
| TTCTGGGCTGCCATCAATGC+TGG | 0.391601 | 4.4:+83635149 | MS.gene89778:CDS |
| GACGCGATCACCAGCATTGA+TGG | 0.392146 | 4.4:-83635159 | None:intergenic |
| GGTTCTATGTCTAGAAGAAT+TGG | 0.399284 | 4.4:+83634619 | MS.gene89778:CDS |
| CATTGATGGCAGCCCAGAAA+AGG | 0.416287 | 4.4:-83635145 | None:intergenic |
| TCGCGTCGAGAGTGCCTTGA+AGG | 0.421679 | 4.4:+83635175 | MS.gene89778:CDS |
| AACTAAAGCCATATCCTTCA+AGG | 0.422487 | 4.4:-83635189 | None:intergenic |
| CAGTTAGTGGATAAAGATCC+AGG | 0.435700 | 4.4:+83635110 | MS.gene89778:intron |
| TTGAACTCTATACGGTATAA+GGG | 0.446484 | 4.4:+83635312 | MS.gene89778:CDS |
| GGAATCTCTTGACAACATCT+TGG | 0.450411 | 4.4:+83635289 | MS.gene89778:CDS |
| TTCTGTTCCAGTCAACAAAC+AGG | 0.461427 | 4.4:+83634675 | MS.gene89778:CDS |
| TCTCTGCCCTTCTGATTCTC+AGG | 0.462514 | 4.4:+83635268 | MS.gene89778:CDS |
| TAGTTATGAAGCAACTGAAC+CGG | 0.474084 | 4.4:+83635207 | MS.gene89778:CDS |
| AAAAGGGAAATTGCTTTACC+TGG | 0.474611 | 4.4:-83635128 | None:intergenic |
| AGCAGAACCTGTTTGTTGAC+TGG | 0.503298 | 4.4:-83634682 | None:intergenic |
| ATTGATGGCAGCCCAGAAAA+GGG | 0.512233 | 4.4:-83635144 | None:intergenic |
| GAGAATCAGAAGGGCAGAGA+TGG | 0.537437 | 4.4:-83635265 | None:intergenic |
| ATTCGATTGCTTCATCCGAC+CGG | 0.551071 | 4.4:-83635226 | None:intergenic |
| CAACATTCTCAGAGGTTCGA+AGG | 0.558149 | 4.4:+83634598 | MS.gene89778:CDS |
| AGAGATTCCTGAGAATCAGA+AGG | 0.602703 | 4.4:-83635275 | None:intergenic |
| GAGATTCCTGAGAATCAGAA+GGG | 0.645235 | 4.4:-83635274 | None:intergenic |
| CACTTCAACAACATTCTCAG+AGG | 0.654400 | 4.4:+83634590 | MS.gene89778:CDS |
| TATGAAGCAACTGAACCGGT+CGG | 0.679096 | 4.4:+83635211 | MS.gene89778:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTCAATTTCTTTTTT+GGG | + | chr4.4:83634709-83634728 | MS.gene89778:intron | 10.0% |
| !!! | TTTTTTTTCAATTTCTTTTT+TGG | + | chr4.4:83634708-83634727 | MS.gene89778:intron | 10.0% |
| !!! | TTTTTGAAGTAATCTTTATC+AGG | + | chr4.4:83635036-83635055 | MS.gene89778:intron | 20.0% |
| ! | ATTTGATAAGTTTCAGAAAG+TGG | + | chr4.4:83634930-83634949 | MS.gene89778:intron | 25.0% |
| ! | TTTGATAAGTTTCAGAAAGT+GGG | + | chr4.4:83634931-83634950 | MS.gene89778:intron | 25.0% |
| !! | TATCAGGTAATTTTCTTTCT+TGG | + | chr4.4:83635052-83635071 | MS.gene89778:intron | 25.0% |
| AATTCATGAGATGTAAGAGA+AGG | + | chr4.4:83634993-83635012 | MS.gene89778:intron | 30.0% | |
| ATTTGATCAGTTTCAGAAAG+TGG | + | chr4.4:83634780-83634799 | MS.gene89778:intron | 30.0% | |
| TCTGAATTCTTTACAGTTAG+TGG | + | chr4.4:83635097-83635116 | MS.gene89778:intron | 30.0% | |
| TTTGATCAGTTTCAGAAAGT+GGG | + | chr4.4:83634781-83634800 | MS.gene89778:intron | 30.0% | |
| ! | TAAAGCAATTTCCCTTTTCT+GGG | + | chr4.4:83635133-83635152 | MS.gene89778:CDS | 30.0% |
| AAAAGGGAAATTGCTTTACC+TGG | - | chr4.4:83635131-83635150 | None:intergenic | 35.0% | |
| AACTAAAGCCATATCCTTCA+AGG | - | chr4.4:83635192-83635211 | None:intergenic | 35.0% | |
| CATCTTGGTTGAACTCTATA+CGG | + | chr4.4:83635304-83635323 | MS.gene89778:CDS | 35.0% | |
| GGTTCTATGTCTAGAAGAAT+TGG | + | chr4.4:83634619-83634638 | MS.gene89778:CDS | 35.0% | |
| TAGTTATGAAGCAACTGAAC+CGG | + | chr4.4:83635207-83635226 | MS.gene89778:CDS | 35.0% | |
| ! | GTAAAGCAATTTCCCTTTTC+TGG | + | chr4.4:83635132-83635151 | MS.gene89778:CDS | 35.0% |
| !!! | TTTTGGGTTTGCATGCTTTT+TGG | + | chr4.4:83634725-83634744 | MS.gene89778:intron | 35.0% |
| AGAGATTCCTGAGAATCAGA+AGG | - | chr4.4:83635278-83635297 | None:intergenic | 40.0% | |
| CACTTCAACAACATTCTCAG+AGG | + | chr4.4:83634590-83634609 | MS.gene89778:CDS | 40.0% | |
| GAGATTCCTGAGAATCAGAA+GGG | - | chr4.4:83635277-83635296 | None:intergenic | 40.0% | |
| GGAATCTCTTGACAACATCT+TGG | + | chr4.4:83635289-83635308 | MS.gene89778:CDS | 40.0% | |
| TTCTGTTCCAGTCAACAAAC+AGG | + | chr4.4:83634675-83634694 | MS.gene89778:CDS | 40.0% | |
| ! | CAGTTAGTGGATAAAGATCC+AGG | + | chr4.4:83635110-83635129 | MS.gene89778:intron | 40.0% |
| ! | TTGTTGACTGGAACAGAATC+AGG | - | chr4.4:83634673-83634692 | None:intergenic | 40.0% |
| ATTCGATTGCTTCATCCGAC+CGG | - | chr4.4:83635229-83635248 | None:intergenic | 45.0% | |
| CAACATTCTCAGAGGTTCGA+AGG | + | chr4.4:83634598-83634617 | MS.gene89778:CDS | 45.0% | |
| TATGAAGCAACTGAACCGGT+CGG | + | chr4.4:83635211-83635230 | MS.gene89778:CDS | 45.0% | |
| ! | AGCAGAACCTGTTTGTTGAC+TGG | - | chr4.4:83634685-83634704 | None:intergenic | 45.0% |
| ! | ATTGATGGCAGCCCAGAAAA+GGG | - | chr4.4:83635147-83635166 | None:intergenic | 45.0% |
| !! | AAAGTGGGTTGTCAGGATTG+TGG | + | chr4.4:83634796-83634815 | MS.gene89778:intron | 45.0% |
| !! | GTTTCAGAAAGTGGGTTGTC+AGG | + | chr4.4:83634789-83634808 | MS.gene89778:intron | 45.0% |
| GAGAATCAGAAGGGCAGAGA+TGG | - | chr4.4:83635268-83635287 | None:intergenic | 50.0% | |
| TCTCTGCCCTTCTGATTCTC+AGG | + | chr4.4:83635268-83635287 | MS.gene89778:CDS | 50.0% | |
| ! | CATTGATGGCAGCCCAGAAA+AGG | - | chr4.4:83635148-83635167 | None:intergenic | 50.0% |
| !! | CGAGAGTGCCTTGAAGGATA+TGG | + | chr4.4:83635181-83635200 | MS.gene89778:CDS | 50.0% |
| TTCTGGGCTGCCATCAATGC+TGG | + | chr4.4:83635149-83635168 | MS.gene89778:CDS | 55.0% | |
| ! | GACGCGATCACCAGCATTGA+TGG | - | chr4.4:83635162-83635181 | None:intergenic | 55.0% |
| !! | TCGCGTCGAGAGTGCCTTGA+AGG | + | chr4.4:83635175-83635194 | MS.gene89778:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 83634586 | 83635331 | 83634586 | ID=MS.gene89778 |
| chr4.4 | mRNA | 83634586 | 83635331 | 83634586 | ID=MS.gene89778.t1;Parent=MS.gene89778 |
| chr4.4 | exon | 83634586 | 83634696 | 83634586 | ID=MS.gene89778.t1.exon1;Parent=MS.gene89778.t1 |
| chr4.4 | CDS | 83634586 | 83634696 | 83634586 | ID=cds.MS.gene89778.t1;Parent=MS.gene89778.t1 |
| chr4.4 | exon | 83635113 | 83635331 | 83635113 | ID=MS.gene89778.t1.exon2;Parent=MS.gene89778.t1 |
| chr4.4 | CDS | 83635113 | 83635331 | 83635113 | ID=cds.MS.gene89778.t1;Parent=MS.gene89778.t1 |
| Gene Sequence |
| Protein sequence |
