Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90003.t1 | XP_013467635.1 | 95.1 | 264 | 8 | 2 | 1 | 264 | 2 | 260 | 6.20E-123 | 450.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90003.t1 | Q6DGZ0 | 30.3 | 277 | 148 | 9 | 14 | 264 | 3 | 260 | 4.4e-16 | 86.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90003.t1 | A0A072VIM1 | 95.1 | 264 | 8 | 2 | 1 | 264 | 2 | 260 | 4.5e-123 | 450.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene90003 | 0.802269 | 6.08E-49 | -1.69E-46 |
| MS.gene050114 | MS.gene90003 | 0.803388 | 3.57E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene90003 | 0.811533 | 6.67E-51 | -1.69E-46 |
| MS.gene054823 | MS.gene90003 | 0.802114 | 6.54E-49 | -1.69E-46 |
| MS.gene056849 | MS.gene90003 | 0.821134 | 4.75E-53 | -1.69E-46 |
| MS.gene056975 | MS.gene90003 | 0.815677 | 8.18E-52 | -1.69E-46 |
| MS.gene057452 | MS.gene90003 | -0.802667 | 5.03E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene90003 | 0.803469 | 3.43E-49 | -1.69E-46 |
| MS.gene058654 | MS.gene90003 | 0.802894 | 4.52E-49 | -1.69E-46 |
| MS.gene06010 | MS.gene90003 | 0.809952 | 1.47E-50 | -1.69E-46 |
| MS.gene060297 | MS.gene90003 | 0.807707 | 4.43E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90003.t1 | MTR_1g053570 | 95.833 | 264 | 6 | 2 | 1 | 264 | 2 | 260 | 0.0 | 508 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90003.t1 | AT3G57910 | 56.777 | 273 | 101 | 4 | 1 | 264 | 1 | 265 | 8.05e-90 | 267 |
Find 77 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACATGGCTGACCTTTCT+AGG | 0.178606 | 1.4:-40257252 | MS.gene90003:CDS |
| CAACAAAATCTTCATCAAAA+AGG | 0.182204 | 1.4:-40257189 | MS.gene90003:CDS |
| TGAATCAAGTTCTGCCCTTT+TGG | 0.263762 | 1.4:-40247291 | MS.gene90003:CDS |
| CCAATGTTCGATTGTGGTAT+TGG | 0.325731 | 1.4:+40248658 | None:intergenic |
| CAATTTCCCGATTCTCCAAT+TGG | 0.343725 | 1.4:+40248356 | None:intergenic |
| CCCTTGACCAATTGGAGAAT+CGG | 0.353036 | 1.4:-40248363 | MS.gene90003:CDS |
| AGGTTCTGCCCTTGGCAAAC+AGG | 0.373865 | 1.4:-40248602 | MS.gene90003:CDS |
| CCCACAAGGAGAAGAAGAAA+AGG | 0.377470 | 1.4:-40248510 | MS.gene90003:CDS |
| GCCAATTGAAAGCTCTTAAC+TGG | 0.377717 | 1.4:-40248756 | MS.gene90003:CDS |
| CAGGCTCTGATGGAAGAATT+CGG | 0.378345 | 1.4:-40248451 | MS.gene90003:CDS |
| CTCTTGGAAGAGTTGATTGA+AGG | 0.396961 | 1.4:+40248778 | None:intergenic |
| CTGAATCAACAACATCTATC+AGG | 0.397846 | 1.4:-40257300 | MS.gene90003:CDS |
| CGACGGTCACGAGCAGGTAT+AGG | 0.404502 | 1.4:-40248544 | MS.gene90003:CDS |
| TGCCCTTGGCAAACAGGGAT+CGG | 0.414677 | 1.4:-40248596 | MS.gene90003:CDS |
| GCCAGTTAAGAGCTTTCAAT+TGG | 0.418381 | 1.4:+40248755 | None:intergenic |
| AAGAGCTTTCAATTGGCTCT+TGG | 0.424774 | 1.4:+40248762 | None:intergenic |
| GCCCTTGGCAAACAGGGATC+GGG | 0.430528 | 1.4:-40248595 | MS.gene90003:CDS |
| TGGTCATCTTCATTGATTCC+AGG | 0.438571 | 1.4:+40247260 | None:intergenic |
| AAATCACAATCGACAGGAAA+AGG | 0.441458 | 1.4:-40248483 | MS.gene90003:CDS |
| AAAGGCTACCCTTGACCAAT+TGG | 0.446594 | 1.4:-40248371 | MS.gene90003:CDS |
| TTGGCAAACAGGGATCGGGC+AGG | 0.449474 | 1.4:-40248591 | MS.gene90003:CDS |
| TGCCCGATCCCTGTTTGCCA+AGG | 0.459471 | 1.4:+40248593 | None:intergenic |
| TCAGCATCATCCTCATTCTT+CGG | 0.462291 | 1.4:+40248325 | None:intergenic |
| CCTTGACCAATTGGAGAATC+GGG | 0.462568 | 1.4:-40248362 | MS.gene90003:CDS |
| TCTCCTTGTGGGGATCCTCT+AGG | 0.462647 | 1.4:+40248521 | None:intergenic |
| GAAGAACGAGCAGGCTCTGA+TGG | 0.471022 | 1.4:-40248461 | MS.gene90003:CDS |
| TGAACAAACCTTGGCAAAAG+TGG | 0.471141 | 1.4:-40248686 | MS.gene90003:CDS |
| TTCAATTACTGCCTCTTCTG+TGG | 0.476483 | 1.4:-40247456 | MS.gene90003:CDS |
| TATACACCAGGTTCTGCCCT+TGG | 0.478103 | 1.4:-40248610 | MS.gene90003:CDS |
| GAAATTCGACGGTCACGAGC+AGG | 0.483570 | 1.4:-40248550 | MS.gene90003:CDS |
| TTCCAGGGCAGTTATCCAAA+AGG | 0.488419 | 1.4:+40247276 | None:intergenic |
| CCCGATTCTCCAATTGGTCA+AGG | 0.492852 | 1.4:+40248362 | None:intergenic |
| ATCGGGCAGGGCTGAACCAG+TGG | 0.511366 | 1.4:-40248578 | MS.gene90003:CDS |
| TGGCAAACAGGGATCGGGCA+GGG | 0.511464 | 1.4:-40248590 | MS.gene90003:CDS |
| ATTTAAGCTTCTCAAACAGA+TGG | 0.513804 | 1.4:-40248635 | MS.gene90003:CDS |
| AGGCTCTGATGGAAGAATTC+GGG | 0.517833 | 1.4:-40248450 | MS.gene90003:CDS |
| TGATGGAAGAATTCGGGTCA+AGG | 0.518913 | 1.4:-40248444 | MS.gene90003:CDS |
| CCACAAGGAGAAGAAGAAAA+GGG | 0.524848 | 1.4:-40248509 | MS.gene90003:CDS |
| ATTGGAGAATCGGGAAATTG+TGG | 0.527475 | 1.4:-40248353 | MS.gene90003:CDS |
| GGTCATCTTCATTGATTCCA+GGG | 0.530782 | 1.4:+40247261 | None:intergenic |
| AGAAGTCACGATGGCAGAGT+AGG | 0.531906 | 1.4:-40248420 | MS.gene90003:CDS |
| AGAGGAAGAAATAACAGAAG+AGG | 0.537870 | 1.4:-40248287 | MS.gene90003:intron |
| TGTTTGCCAAGGGCAGAACC+TGG | 0.540602 | 1.4:+40248604 | None:intergenic |
| TGTTAATTTCAACAAGGCAA+AGG | 0.545569 | 1.4:-40248389 | MS.gene90003:CDS |
| GCCCGATCCCTGTTTGCCAA+GGG | 0.546633 | 1.4:+40248594 | None:intergenic |
| TTTAAGCTTCTCAAACAGAT+GGG | 0.551840 | 1.4:-40248634 | MS.gene90003:CDS |
| GGGAGGAAATCACAATCGAC+AGG | 0.554805 | 1.4:-40248489 | MS.gene90003:CDS |
| TCCAGGGCAGTTATCCAAAA+GGG | 0.557950 | 1.4:+40247277 | None:intergenic |
| TCACTTTGCATCCACAGAAG+AGG | 0.562538 | 1.4:+40247445 | None:intergenic |
| ACAAGAGGATGAACAAACCT+TGG | 0.566502 | 1.4:-40248695 | MS.gene90003:CDS |
| CGGAAGGAAGAAACCTAGAA+AGG | 0.570557 | 1.4:+40257239 | None:intergenic |
| GGTTCTGCCCTTGGCAAACA+GGG | 0.570825 | 1.4:-40248601 | MS.gene90003:CDS |
| TTAAATCCAATGTTCGATTG+TGG | 0.577496 | 1.4:+40248652 | None:intergenic |
| AGAAAGAAAGCAAATACAAG+AGG | 0.580568 | 1.4:-40248710 | MS.gene90003:CDS |
| CGTCGAATTTCAATACCCAC+TGG | 0.583668 | 1.4:+40248562 | None:intergenic |
| CCGATTCTCCAATTGGTCAA+GGG | 0.583969 | 1.4:+40248363 | None:intergenic |
| AAGCTCTTAACTGGCAAGAA+CGG | 0.587734 | 1.4:-40248747 | MS.gene90003:CDS |
| TGTGGAACCACCGAAGAATG+AGG | 0.589311 | 1.4:-40248335 | MS.gene90003:CDS |
| TCGGGCAGGGCTGAACCAGT+GGG | 0.592431 | 1.4:-40248577 | MS.gene90003:CDS |
| CCAATACCACAATCGAACAT+TGG | 0.594956 | 1.4:-40248658 | MS.gene90003:CDS |
| GCATCATCCTCATTCTTCGG+TGG | 0.598461 | 1.4:+40248328 | None:intergenic |
| TGAGGGTGAAGATCAAGAAG+AGG | 0.599844 | 1.4:-40248305 | MS.gene90003:CDS |
| TGATGATAACGATGACTACA+TGG | 0.603022 | 1.4:-40257266 | MS.gene90003:CDS |
| CAGGAAAAGGAAGAACGAGC+AGG | 0.604352 | 1.4:-40248470 | MS.gene90003:CDS |
| CAAGGAGAAGAAGAAAAGGG+AGG | 0.611846 | 1.4:-40248506 | MS.gene90003:CDS |
| AGGATGCATCATAGCATCAT+CGG | 0.624936 | 1.4:+40257219 | None:intergenic |
| AAACAGATGGGTTATACACC+AGG | 0.633040 | 1.4:-40248622 | MS.gene90003:CDS |
| CGATGTATTAATGAAACTGA+GGG | 0.643502 | 1.4:-40247484 | MS.gene90003:CDS |
| TGCATCATAGCATCATCGGA+AGG | 0.647809 | 1.4:+40257223 | None:intergenic |
| TCGATGTATTAATGAAACTG+AGG | 0.650645 | 1.4:-40247485 | MS.gene90003:CDS |
| CAGTGGGTATTGAAATTCGA+CGG | 0.653278 | 1.4:-40248561 | MS.gene90003:CDS |
| AGTTATTGTTAATTTCAACA+AGG | 0.655513 | 1.4:-40248395 | MS.gene90003:CDS |
| GAAGAATGAGGATGATGCTG+AGG | 0.657530 | 1.4:-40248323 | MS.gene90003:CDS |
| AGGCCTAGAGGATCCCCACA+AGG | 0.671378 | 1.4:-40248524 | MS.gene90003:CDS |
| ACGAGCAGGTATAGGCCTAG+AGG | 0.682619 | 1.4:-40248536 | MS.gene90003:CDS |
| AAGAATGAGGATGATGCTGA+GGG | 0.717485 | 1.4:-40248322 | MS.gene90003:CDS |
| GGTCAAGGCAGAAGTCACGA+TGG | 0.740710 | 1.4:-40248429 | MS.gene90003:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 40247256 | 40257326 | 40247256 | ID=MS.gene90003 |
| chr1.4 | mRNA | 40247256 | 40257326 | 40247256 | ID=MS.gene90003.t1;Parent=MS.gene90003 |
| chr1.4 | exon | 40257189 | 40257326 | 40257189 | ID=MS.gene90003.t1.exon1;Parent=MS.gene90003.t1 |
| chr1.4 | CDS | 40257189 | 40257326 | 40257189 | ID=cds.MS.gene90003.t1;Parent=MS.gene90003.t1 |
| chr1.4 | exon | 40248288 | 40248818 | 40248288 | ID=MS.gene90003.t1.exon2;Parent=MS.gene90003.t1 |
| chr1.4 | CDS | 40248288 | 40248818 | 40248288 | ID=cds.MS.gene90003.t1;Parent=MS.gene90003.t1 |
| chr1.4 | exon | 40247449 | 40247514 | 40247449 | ID=MS.gene90003.t1.exon3;Parent=MS.gene90003.t1 |
| chr1.4 | CDS | 40247449 | 40247514 | 40247449 | ID=cds.MS.gene90003.t1;Parent=MS.gene90003.t1 |
| chr1.4 | exon | 40247256 | 40247315 | 40247256 | ID=MS.gene90003.t1.exon4;Parent=MS.gene90003.t1 |
| chr1.4 | CDS | 40247256 | 40247315 | 40247256 | ID=cds.MS.gene90003.t1;Parent=MS.gene90003.t1 |
| Gene Sequence |
| Protein sequence |
