Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90223.t1 | XP_003608396.1 | 98.8 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 6.90E-39 | 169.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90223.t1 | A2XKU9 | 81.2 | 85 | 16 | 0 | 1 | 85 | 1 | 85 | 3.2e-32 | 138.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90223.t1 | G7JV45 | 98.8 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 5.0e-39 | 169.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene90223 | 0.802081 | 6.64E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene90223 | 0.820725 | 5.89E-53 | -1.69E-46 |
| MS.gene050838 | MS.gene90223 | 0.81006 | 1.39E-50 | -1.69E-46 |
| MS.gene052133 | MS.gene90223 | 0.801712 | 7.91E-49 | -1.69E-46 |
| MS.gene052335 | MS.gene90223 | 0.817199 | 3.73E-52 | -1.69E-46 |
| MS.gene052732 | MS.gene90223 | 0.803839 | 2.88E-49 | -1.69E-46 |
| MS.gene053143 | MS.gene90223 | 0.801944 | 7.09E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene90223 | 0.821828 | 3.28E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene90223 | 0.892444 | 1.58E-74 | -1.69E-46 |
| MS.gene054584 | MS.gene90223 | 0.800267 | 1.56E-48 | -1.69E-46 |
| MS.gene055468 | MS.gene90223 | 0.805794 | 1.12E-49 | -1.69E-46 |
| MS.gene058852 | MS.gene90223 | 0.813784 | 2.15E-51 | -1.69E-46 |
| MS.gene058945 | MS.gene90223 | 0.811041 | 8.54E-51 | -1.69E-46 |
| MS.gene059171 | MS.gene90223 | 0.816663 | 4.92E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90223.t1 | MTR_4g093660 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 4.01e-58 | 172 |
| MS.gene90223.t1 | MTR_2g009660 | 85.882 | 85 | 12 | 0 | 1 | 85 | 63 | 147 | 2.55e-50 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90223.t1 | AT4G33640 | 80.769 | 78 | 15 | 0 | 1 | 78 | 1 | 78 | 1.09e-41 | 132 |
| MS.gene90223.t1 | AT4G33640 | 80.769 | 78 | 15 | 0 | 1 | 78 | 67 | 144 | 3.34e-41 | 132 |
Find 17 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGTGAAGGTCACATT+TGG | 0.398089 | 4.2:-19886276 | MS.gene90223:intron |
| GAGTGATTTCCACATTATCA+TGG | 0.446001 | 4.2:+19886136 | None:intergenic |
| AATATATTTGAAGCACTTGT+CGG | 0.467086 | 4.2:-19886225 | MS.gene90223:CDS |
| GAACACTCTTTCACGATGAT+CGG | 0.491735 | 4.2:-19886254 | MS.gene90223:CDS |
| AAGAGGAGGTTGAAAAGCTG+AGG | 0.496528 | 4.2:-19887020 | MS.gene90223:CDS |
| CCGAATCAGTTAGCAGCAGC+AGG | 0.502169 | 4.2:+19886102 | None:intergenic |
| CAGTTAGCAGCAGCAGGAGC+AGG | 0.504867 | 4.2:+19886108 | None:intergenic |
| CTTGGAAATGTTCAAGCAGA+TGG | 0.516658 | 4.2:-19886982 | MS.gene90223:CDS |
| AGGGAAGAAATCAAGAGGCT+TGG | 0.527446 | 4.2:-19887000 | MS.gene90223:CDS |
| AGAGGAGGTTGAAAAGCTGA+GGG | 0.572997 | 4.2:-19887019 | MS.gene90223:CDS |
| TGTGAAAATGAACGTAGAAG+AGG | 0.578780 | 4.2:-19887037 | None:intergenic |
| TCAAGCAGATGGTTCTTGCA+AGG | 0.604584 | 4.2:-19886971 | MS.gene90223:intron |
| AGCTGAGGGAAGAAATCAAG+AGG | 0.626324 | 4.2:-19887005 | MS.gene90223:CDS |
| GACGGTGAACTACTGTTGCA+AGG | 0.632747 | 4.2:-19886162 | MS.gene90223:CDS |
| GAAAATGAACGTAGAAGAGG+AGG | 0.643787 | 4.2:-19887034 | None:intergenic |
| GCAAGGAGTCCATGATAATG+TGG | 0.676630 | 4.2:-19886145 | MS.gene90223:CDS |
| CGTAAAGTAGTTGCATACGA+CGG | 0.749247 | 4.2:-19886180 | MS.gene90223:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAAACAAATTAATGTAT+AGG | + | chr4.2:19886478-19886497 | None:intergenic | 10.0% |
| !! | ATAATTGTAATTAAATGTGT+TGG | - | chr4.2:19886647-19886666 | MS.gene90223:intron | 15.0% |
| !!! | AATTTGTTTATTATAAACCA+TGG | - | chr4.2:19886485-19886504 | MS.gene90223:intron | 15.0% |
| !! | GAATCAAGTTCTTAATTAAA+AGG | + | chr4.2:19886272-19886291 | None:intergenic | 20.0% |
| !! | TTTCATATGATATTTCGTAA+TGG | + | chr4.2:19886405-19886424 | None:intergenic | 20.0% |
| !!! | AAGTATTGATATGATGTTTA+GGG | + | chr4.2:19886612-19886631 | None:intergenic | 20.0% |
| !!! | TAAGTATTGATATGATGTTT+AGG | + | chr4.2:19886613-19886632 | None:intergenic | 20.0% |
| ! | ATGCATCATGAAAATGTATA+TGG | + | chr4.2:19886234-19886253 | None:intergenic | 25.0% |
| ! | CATTCCATATAAAACTGATA+TGG | - | chr4.2:19886531-19886550 | MS.gene90223:intron | 25.0% |
| ! | TAATGTATAGGTAGATACAT+TGG | + | chr4.2:19886466-19886485 | None:intergenic | 25.0% |
| ! | TCAGATCTAGATATATTGAT+GGG | - | chr4.2:19886290-19886309 | MS.gene90223:intron | 25.0% |
| ! | TTCAGATCTAGATATATTGA+TGG | - | chr4.2:19886289-19886308 | MS.gene90223:intron | 25.0% |
| ! | TTGTTTATTATAAACCATGG+AGG | - | chr4.2:19886488-19886507 | MS.gene90223:intron | 25.0% |
| !! | AATATATTTGAAGCACTTGT+CGG | - | chr4.2:19886912-19886931 | MS.gene90223:intron | 25.0% |
| !! | GAAAATGTATATGGTTTGTA+AGG | + | chr4.2:19886225-19886244 | None:intergenic | 25.0% |
| !!! | AATTTGCTGAAATAGTACTA+TGG | - | chr4.2:19886707-19886726 | MS.gene90223:intron | 25.0% |
| !!! | CATATCAGTTTTATATGGAA+TGG | + | chr4.2:19886533-19886552 | None:intergenic | 25.0% |
| AAGTATGTAATGCATTGTGA+AGG | - | chr4.2:19886851-19886870 | MS.gene90223:intron | 30.0% | |
| TATAGTGAGTGATGGTTAAT+TGG | - | chr4.2:19886757-19886776 | MS.gene90223:intron | 30.0% | |
| TTCTTGCAAGGTATAACAAA+AGG | - | chr4.2:19886178-19886197 | MS.gene90223:CDS | 30.0% | |
| ! | CTATATCTTCAGTTGATTCA+TGG | + | chr4.2:19886742-19886761 | None:intergenic | 30.0% |
| ! | TGATGGGTATTTATTGAATG+TGG | - | chr4.2:19886306-19886325 | MS.gene90223:intron | 30.0% |
| ! | TGGTCACTTTCTTATTCTTA+TGG | - | chr4.2:19886326-19886345 | MS.gene90223:intron | 30.0% |
| !! | CTAGCCATATCAGTTTTATA+TGG | + | chr4.2:19886538-19886557 | None:intergenic | 30.0% |
| ACTGAAGATATAGTGAGTGA+TGG | - | chr4.2:19886749-19886768 | MS.gene90223:intron | 35.0% | |
| GAGTGATTTCCACATTATCA+TGG | + | chr4.2:19887004-19887023 | None:intergenic | 35.0% | |
| GTTAATGCGCATATGTGAAT+TGG | - | chr4.2:19886787-19886806 | MS.gene90223:intron | 35.0% | |
| TCTTATGGATCTAGATCACA+AGG | - | chr4.2:19886341-19886360 | MS.gene90223:intron | 35.0% | |
| CACAAGGTCATTCACAACAT+TGG | - | chr4.2:19886357-19886376 | MS.gene90223:intron | 40.0% | |
| CGTAAAGTAGTTGCATACGA+CGG | - | chr4.2:19886957-19886976 | MS.gene90223:intron | 40.0% | |
| CTTGGAAATGTTCAAGCAGA+TGG | - | chr4.2:19886155-19886174 | MS.gene90223:CDS | 40.0% | |
| GAACACTCTTTCACGATGAT+CGG | - | chr4.2:19886883-19886902 | MS.gene90223:intron | 40.0% | |
| TGCATTGTGAAGGTCACATT+TGG | - | chr4.2:19886861-19886880 | MS.gene90223:intron | 40.0% | |
| AAGAGGAGGTTGAAAAGCTG+AGG | - | chr4.2:19886117-19886136 | MS.gene90223:CDS | 45.0% | |
| AGAGGAGGTTGAAAAGCTGA+GGG | - | chr4.2:19886118-19886137 | MS.gene90223:CDS | 45.0% | |
| AGCTGAGGGAAGAAATCAAG+AGG | - | chr4.2:19886132-19886151 | MS.gene90223:CDS | 45.0% | |
| AGGGAAGAAATCAAGAGGCT+TGG | - | chr4.2:19886137-19886156 | MS.gene90223:CDS | 45.0% | |
| TCAAGCAGATGGTTCTTGCA+AGG | - | chr4.2:19886166-19886185 | MS.gene90223:CDS | 45.0% | |
| ! | GCAAGGAGTCCATGATAATG+TGG | - | chr4.2:19886992-19887011 | MS.gene90223:CDS | 45.0% |
| CGCATGATATCTCTCCTCCA+TGG | + | chr4.2:19886505-19886524 | None:intergenic | 50.0% | |
| GACGGTGAACTACTGTTGCA+AGG | - | chr4.2:19886975-19886994 | MS.gene90223:CDS | 50.0% | |
| CAGTTAGCAGCAGCAGGAGC+AGG | + | chr4.2:19887032-19887051 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 19886107 | 19887052 | 19886107 | ID=MS.gene90223 |
| chr4.2 | mRNA | 19886107 | 19887052 | 19886107 | ID=MS.gene90223.t1;Parent=MS.gene90223 |
| chr4.2 | exon | 19886972 | 19887052 | 19886972 | ID=MS.gene90223.t1.exon1;Parent=MS.gene90223.t1 |
| chr4.2 | CDS | 19886972 | 19887052 | 19886972 | ID=cds.MS.gene90223.t1;Parent=MS.gene90223.t1 |
| chr4.2 | exon | 19886107 | 19886286 | 19886107 | ID=MS.gene90223.t1.exon2;Parent=MS.gene90223.t1 |
| chr4.2 | CDS | 19886107 | 19886286 | 19886107 | ID=cds.MS.gene90223.t1;Parent=MS.gene90223.t1 |
| Gene Sequence |
| Protein sequence |
