AlfalfaGEDB | MS.gene058945

M. sativa cultivar XinJiangDaYe / MS.gene058945

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058945.t1	XP_003597234.1	93.5	139	7	2	1	138	1	138	2.50E-62	248.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058945.t1	G7IRR9	93.5	139	7	2	1	138	1	138	1.8e-62	248.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051719	MS.gene058945	0.813827	2.10E-51	-1.69E-46
MS.gene051720	MS.gene058945	0.803919	2.77E-49	-1.69E-46
MS.gene052133	MS.gene058945	0.801341	9.42E-49	-1.69E-46
MS.gene052516	MS.gene058945	0.803692	3.09E-49	-1.69E-46
MS.gene052732	MS.gene058945	0.827007	1.98E-54	-1.69E-46
MS.gene053144	MS.gene058945	0.839751	1.31E-57	-1.69E-46
MS.gene053581	MS.gene058945	0.854829	9.48E-62	-1.69E-46
MS.gene054584	MS.gene058945	0.828128	1.07E-54	-1.69E-46
MS.gene055465	MS.gene058945	0.810222	1.28E-50	-1.69E-46
MS.gene055537	MS.gene058945	0.814804	1.28E-51	-1.69E-46
MS.gene055891	MS.gene058945	0.818397	2.00E-52	-1.69E-46
MS.gene058339	MS.gene058945	0.800044	1.73E-48	-1.69E-46
MS.gene058945	MS.gene062821	0.8074	5.15E-50	-1.69E-46
MS.gene058945	MS.gene063452	0.829045	6.41E-55	-1.69E-46
MS.gene058945	MS.gene068022	0.850987	1.19E-60	-1.69E-46
MS.gene058945	MS.gene068659	0.800037	1.74E-48	-1.69E-46
MS.gene058945	MS.gene068925	0.807208	5.65E-50	-1.69E-46
MS.gene058945	MS.gene071096	0.807663	4.53E-50	-1.69E-46
MS.gene058945	MS.gene073083	0.801453	8.94E-49	-1.69E-46
MS.gene058945	MS.gene07359	0.839911	1.19E-57	-1.69E-46
MS.gene058945	MS.gene074337	0.826321	2.89E-54	-1.69E-46
MS.gene058945	MS.gene074435	0.820869	5.46E-53	-1.69E-46
MS.gene058945	MS.gene074519	0.838796	2.32E-57	-1.69E-46
MS.gene058945	MS.gene20061	0.810483	1.13E-50	-1.69E-46
MS.gene058945	MS.gene20903	0.86055	1.91E-63	-1.69E-46
MS.gene058945	MS.gene22436	0.823517	1.33E-53	-1.69E-46
MS.gene058945	MS.gene25435	0.826787	2.24E-54	-1.69E-46
MS.gene058945	MS.gene25436	0.827424	1.58E-54	-1.69E-46
MS.gene058945	MS.gene25810	0.818423	1.98E-52	-1.69E-46
MS.gene058945	MS.gene26380	0.807453	5.02E-50	-1.69E-46
MS.gene058945	MS.gene26659	0.814313	1.64E-51	-1.69E-46
MS.gene058945	MS.gene26851	0.825545	4.42E-54	-1.69E-46
MS.gene058945	MS.gene29957	0.805113	1.56E-49	-1.69E-46
MS.gene058945	MS.gene30419	0.808818	2.57E-50	-1.69E-46
MS.gene058945	MS.gene30905	0.814114	1.82E-51	-1.69E-46
MS.gene058945	MS.gene31203	0.828485	8.76E-55	-1.69E-46
MS.gene058945	MS.gene31204	0.832565	8.79E-56	-1.69E-46
MS.gene058945	MS.gene31294	0.833783	4.37E-56	-1.69E-46
MS.gene058945	MS.gene34286	0.839955	1.16E-57	-1.69E-46
MS.gene058945	MS.gene38303	0.83539	1.73E-56	-1.69E-46
MS.gene058945	MS.gene38561	0.828874	7.05E-55	-1.69E-46
MS.gene058945	MS.gene38693	0.81545	9.19E-52	-1.69E-46
MS.gene058945	MS.gene40739	0.817663	2.93E-52	-1.69E-46
MS.gene058945	MS.gene41651	0.807818	4.20E-50	-1.69E-46
MS.gene058945	MS.gene41757	0.808133	3.60E-50	-1.69E-46
MS.gene058945	MS.gene43075	0.814693	1.35E-51	-1.69E-46
MS.gene058945	MS.gene44844	0.805589	1.24E-49	-1.69E-46
MS.gene058945	MS.gene53249	0.804869	1.76E-49	-1.69E-46
MS.gene058945	MS.gene53280	0.808857	2.52E-50	-1.69E-46
MS.gene058945	MS.gene56462	0.822372	2.45E-53	-1.69E-46
MS.gene058945	MS.gene57609	0.806391	8.42E-50	-1.69E-46
MS.gene058945	MS.gene58214	0.80394	2.74E-49	-1.69E-46
MS.gene058945	MS.gene59294	0.815804	7.66E-52	-1.69E-46
MS.gene058945	MS.gene60565	0.822507	2.28E-53	-1.69E-46
MS.gene058945	MS.gene62806	0.818729	1.68E-52	-1.69E-46
MS.gene058945	MS.gene65276	0.820948	5.24E-53	-1.69E-46
MS.gene058945	MS.gene68418	0.806448	8.19E-50	-1.69E-46
MS.gene058945	MS.gene70730	0.836178	1.09E-56	-1.69E-46
MS.gene058945	MS.gene71151	0.800201	1.61E-48	-1.69E-46
MS.gene058945	MS.gene72272	0.809155	2.18E-50	-1.69E-46
MS.gene058945	MS.gene72986	0.805107	1.57E-49	-1.69E-46
MS.gene058945	MS.gene73327	0.822121	2.81E-53	-1.69E-46
MS.gene058945	MS.gene78189	0.810396	1.18E-50	-1.69E-46
MS.gene058945	MS.gene81678	0.806675	7.33E-50	-1.69E-46
MS.gene058945	MS.gene84426	0.807871	4.09E-50	-1.69E-46
MS.gene058945	MS.gene88718	0.815015	1.15E-51	-1.69E-46
MS.gene058945	MS.gene89974	0.812519	4.07E-51	-1.69E-46
MS.gene058945	MS.gene90223	0.811041	8.54E-51	-1.69E-46
MS.gene058945	MS.gene90610	0.822438	2.37E-53	-1.69E-46
MS.gene058945	MS.gene90646	0.805044	1.61E-49	-1.69E-46
MS.gene058945	MS.gene90649	0.815698	8.09E-52	-1.69E-46
MS.gene058945	MS.gene91933	0.804849	1.77E-49	-1.69E-46
MS.gene058945	MS.gene95401	0.812309	4.52E-51	-1.69E-46
MS.gene058945	MS.gene97761	0.816122	6.51E-52	-1.69E-46
MS.gene058945	MS.gene99595	0.805526	1.28E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058945.t1	MTR_2g094210	93.525	139	7	2	1	138	1	138	2.17e-90	258
MS.gene058945.t1	MTR_4g059310	54.867	113	44	3	17	127	19	126	3.63e-36	121

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058945.t1	AT2G28410	43.119	109	55	3	7	110	9	115	9.53e-23	86.7
MS.gene058945.t1	AT5G60650	32.609	138	80	4	7	133	10	145	4.30e-13	62.8

Find 26 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGAAAATGCTTCAGGATTT+TGG	0.150554	2.3:-7571695	None:intergenic
GGCATTGTTGGAATTATCTT+TGG	0.299173	2.3:+7571891	MS.gene058945:CDS
AGACAAAGGTAGGAAACATT+TGG	0.333054	2.3:+7571860	MS.gene058945:CDS
TTGGGAGCTGGTGGCATTGT+TGG	0.397431	2.3:+7571879	MS.gene058945:CDS
TTGAACACTATTATTAGCTT+TGG	0.406861	2.3:-7571988	None:intergenic
TGAGTAGCTTCTACAGCTAA+AGG	0.425538	2.3:-7571810	None:intergenic
GCTGATGGTGAAAATGCTTC+AGG	0.426528	2.3:-7571702	None:intergenic
GACAAAGGTAGGAAACATTT+GGG	0.431572	2.3:+7571861	MS.gene058945:CDS
AGCTTTGGCTCTAATCATGT+TGG	0.475963	2.3:-7571973	None:intergenic
AGCTTCTACAGCTAAAGGTA+AGG	0.481895	2.3:-7571805	None:intergenic
AAAGGCAAGGTACATCAAAC+AGG	0.497433	2.3:-7572014	None:intergenic
GTGGAAAATTAGACAAAGGT+AGG	0.516928	2.3:+7571850	MS.gene058945:CDS
CTTTGGTGTTGCATTTGTTG+TGG	0.527586	2.3:+7571908	MS.gene058945:CDS
TAGTGACAAGTGATAGAGAA+AGG	0.533134	2.3:-7571632	None:intergenic
GGTAGGAAACATTTGGGAGC+TGG	0.541255	2.3:+7571867	MS.gene058945:CDS
CAAATTCATGAAAGCAAAGG+TGG	0.545860	2.3:+7571831	MS.gene058945:CDS
CATCCCAATTCTCAAAAGCA+TGG	0.558982	2.3:+7571738	MS.gene058945:CDS
GCTTCTACAGCTAAAGGTAA+GGG	0.562499	2.3:-7571804	None:intergenic
TCTCAAGAAAGAGCACCTCA+TGG	0.585347	2.3:+7571663	MS.gene058945:CDS
ACTCAAATTCATGAAAGCAA+AGG	0.598332	2.3:+7571828	MS.gene058945:CDS
TGACAAGTGATAGAGAAAGG+AGG	0.600215	2.3:-7571629	None:intergenic
TCTAACATGATACACACTCA+TGG	0.618148	2.3:-7571937	None:intergenic
TGAAAGAATTCATATGCTGA+TGG	0.627715	2.3:-7571717	None:intergenic
AGGAAACATTTGGGAGCTGG+TGG	0.627993	2.3:+7571870	MS.gene058945:CDS
AAAGGTGGAAAATTAGACAA+AGG	0.635337	2.3:+7571846	MS.gene058945:CDS
GGCAAGGTACATCAAACAGG+TGG	0.689975	2.3:-7572011	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGAACACTATTATTAGCTT+TGG	-	chr2.3:7571991-7572010	None:intergenic	25.0%
	AAAGGTGGAAAATTAGACAA+AGG	+	chr2.3:7571846-7571865	MS.gene058945:CDS	30.0%
	ACTCAAATTCATGAAAGCAA+AGG	+	chr2.3:7571828-7571847	MS.gene058945:CDS	30.0%
	TGAAAGAATTCATATGCTGA+TGG	-	chr2.3:7571720-7571739	None:intergenic	30.0%
!!	ATTACCATGCTTTTGAGAAT+TGG	-	chr2.3:7571745-7571764	None:intergenic	30.0%
!!	TTACCATGCTTTTGAGAATT+GGG	-	chr2.3:7571744-7571763	None:intergenic	30.0%
	AGACAAAGGTAGGAAACATT+TGG	+	chr2.3:7571860-7571879	MS.gene058945:CDS	35.0%
	CAAATTCATGAAAGCAAAGG+TGG	+	chr2.3:7571831-7571850	MS.gene058945:CDS	35.0%
	GACAAAGGTAGGAAACATTT+GGG	+	chr2.3:7571861-7571880	MS.gene058945:CDS	35.0%
	GGCATTGTTGGAATTATCTT+TGG	+	chr2.3:7571891-7571910	MS.gene058945:CDS	35.0%
	GTGGAAAATTAGACAAAGGT+AGG	+	chr2.3:7571850-7571869	MS.gene058945:CDS	35.0%
	TAGTGACAAGTGATAGAGAA+AGG	-	chr2.3:7571635-7571654	None:intergenic	35.0%
	TCTAACATGATACACACTCA+TGG	-	chr2.3:7571940-7571959	None:intergenic	35.0%
!	GTGAAAATGCTTCAGGATTT+TGG	-	chr2.3:7571698-7571717	None:intergenic	35.0%
!!	GAACATTTTGATGAAGTGCA+TGG	-	chr2.3:7571783-7571802	None:intergenic	35.0%
!!	TTTTGGTTAACAAGTCCATG+AGG	-	chr2.3:7571681-7571700	None:intergenic	35.0%
	AGCTTCTACAGCTAAAGGTA+AGG	-	chr2.3:7571808-7571827	None:intergenic	40.0%
	CATCCCAATTCTCAAAAGCA+TGG	+	chr2.3:7571738-7571757	MS.gene058945:CDS	40.0%
	GCTTCTACAGCTAAAGGTAA+GGG	-	chr2.3:7571807-7571826	None:intergenic	40.0%
	TGACAAGTGATAGAGAAAGG+AGG	-	chr2.3:7571632-7571651	None:intergenic	40.0%
	TGAGTAGCTTCTACAGCTAA+AGG	-	chr2.3:7571813-7571832	None:intergenic	40.0%
!!	AGCTTTGGCTCTAATCATGT+TGG	-	chr2.3:7571976-7571995	None:intergenic	40.0%
!!	CTTTGGTGTTGCATTTGTTG+TGG	+	chr2.3:7571908-7571927	MS.gene058945:CDS	40.0%
!	GCTGATGGTGAAAATGCTTC+AGG	-	chr2.3:7571705-7571724	None:intergenic	45.0%
!!	TCTCAAGAAAGAGCACCTCA+TGG	+	chr2.3:7571663-7571682	MS.gene058945:CDS	45.0%
	AGGAAACATTTGGGAGCTGG+TGG	+	chr2.3:7571870-7571889	MS.gene058945:CDS	50.0%
	GGTAGGAAACATTTGGGAGC+TGG	+	chr2.3:7571867-7571886	MS.gene058945:CDS	50.0%
	TTGGGAGCTGGTGGCATTGT+TGG	+	chr2.3:7571879-7571898	MS.gene058945:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	7571606	7572022	7571606	ID=MS.gene058945
chr2.3	mRNA	7571606	7572022	7571606	ID=MS.gene058945.t1;Parent=MS.gene058945
chr2.3	exon	7571606	7572022	7571606	ID=MS.gene058945.t1.exon1;Parent=MS.gene058945.t1
chr2.3	CDS	7571606	7572022	7571606	ID=cds.MS.gene058945.t1;Parent=MS.gene058945.t1

Gene Sequence

>MS.gene058945

ATGATCAATCACATTGGTTTCTTCCTCCTTTCTCTATCACTTGTCACTATTGTTACATCTCAAGAAAGAGCACCTCATGGACTTGTTAACCAAAATCCTGAAGCATTTTCACCATCAGCATATGAATTCTTTCATCCCAATTCTCAAAAGCATGGTAATGACAAAAAAGATGATCCATGCACTTCATCAAAATGTTCACCCTTACCTTTAGCTGTAGAAGCTACTCAAATTCATGAAAGCAAAGGTGGAAAATTAGACAAAGGTAGGAAACATTTGGGAGCTGGTGGCATTGTTGGAATTATCTTTGGTGTTGCATTTGTTGTGGTTTTAGCCATGAGTGTGTATCATGTTAGAGTCACACGTAAAGCCAACATGATTAGAGCCAAAGCTAATAATAGTGTTCAACCACCTGTTTGA

Protein sequence

>MS.gene058945.t1

MINHIGFFLLSLSLVTIVTSQERAPHGLVNQNPEAFSPSAYEFFHPNSQKHGNDKKDDPCTSSKCSPLPLAVEATQIHESKGGKLDKGRKHLGAGGIVGIIFGVAFVVVLAMSVYHVRVTRKANMIRAKANNSVQPPV