Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90337.t1 | XP_003622999.2 | 67.7 | 439 | 79 | 4 | 1 | 379 | 2 | 437 | 4.70E-151 | 544.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90337.t1 | P0C897 | 23.5 | 200 | 147 | 5 | 178 | 375 | 313 | 508 | 7.6e-09 | 63.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90337.t1 | G7L2D6 | 67.7 | 439 | 79 | 4 | 1 | 379 | 2 | 437 | 3.4e-151 | 544.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051247 | MS.gene90337 | 0.800113 | 1.68E-48 | -1.69E-46 |
| MS.gene051265 | MS.gene90337 | 0.813791 | 2.14E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene90337 | 0.808037 | 3.77E-50 | -1.69E-46 |
| MS.gene05468 | MS.gene90337 | 0.806701 | 7.24E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene90337 | 0.830973 | 2.17E-55 | -1.69E-46 |
| MS.gene054801 | MS.gene90337 | 0.80451 | 2.09E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene90337 | 0.81117 | 8.00E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene90337 | 0.817095 | 3.94E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90337.t1 | MTR_7g060220 | 64.066 | 423 | 87 | 6 | 1 | 366 | 2 | 416 | 4.33e-179 | 505 |
| MS.gene90337.t1 | MTR_7g060840 | 59.811 | 423 | 122 | 6 | 1 | 376 | 1 | 422 | 5.33e-168 | 477 |
| MS.gene90337.t1 | MTR_7g060260 | 61.538 | 403 | 114 | 4 | 1 | 363 | 2 | 403 | 5.15e-161 | 459 |
| MS.gene90337.t1 | MTR_7g060020 | 57.009 | 428 | 124 | 7 | 2 | 379 | 3 | 420 | 1.19e-155 | 446 |
| MS.gene90337.t1 | MTR_7g060160 | 56.028 | 423 | 111 | 6 | 1 | 379 | 1 | 392 | 2.82e-145 | 418 |
| MS.gene90337.t1 | MTR_0182s0060 | 80.526 | 190 | 37 | 0 | 190 | 379 | 320 | 509 | 2.69e-104 | 318 |
| MS.gene90337.t1 | MTR_0182s0060 | 58.150 | 227 | 73 | 2 | 1 | 205 | 2 | 228 | 9.24e-73 | 236 |
| MS.gene90337.t1 | MTR_7g060115 | 78.534 | 191 | 40 | 1 | 1 | 191 | 1 | 190 | 4.69e-97 | 297 |
| MS.gene90337.t1 | MTR_7g060115 | 53.881 | 219 | 85 | 6 | 168 | 379 | 231 | 440 | 1.56e-63 | 210 |
| MS.gene90337.t1 | MTR_7g059810 | 73.762 | 202 | 50 | 2 | 168 | 366 | 223 | 424 | 4.82e-97 | 296 |
| MS.gene90337.t1 | MTR_7g059810 | 75.916 | 191 | 43 | 2 | 1 | 191 | 2 | 189 | 2.99e-91 | 282 |
| MS.gene90337.t1 | MTR_7g060320 | 79.581 | 191 | 37 | 2 | 1 | 191 | 2 | 190 | 7.77e-96 | 294 |
| MS.gene90337.t1 | MTR_7g060320 | 58.294 | 211 | 73 | 4 | 168 | 363 | 231 | 441 | 4.01e-72 | 233 |
| MS.gene90337.t1 | MTR_7g060310 | 74.627 | 201 | 48 | 2 | 2 | 200 | 4 | 203 | 4.02e-95 | 293 |
| MS.gene90337.t1 | MTR_7g060310 | 56.682 | 217 | 86 | 4 | 168 | 379 | 267 | 480 | 1.77e-73 | 237 |
| MS.gene90337.t1 | MTR_7g059480 | 79.058 | 191 | 38 | 2 | 1 | 190 | 1 | 190 | 2.75e-92 | 293 |
| MS.gene90337.t1 | MTR_7g059480 | 60.638 | 188 | 71 | 2 | 195 | 379 | 579 | 766 | 3.53e-69 | 232 |
| MS.gene90337.t1 | MTR_7g059515 | 75.401 | 187 | 42 | 2 | 1 | 187 | 1 | 183 | 9.03e-92 | 275 |
| MS.gene90337.t1 | MTR_7g060370 | 71.053 | 190 | 53 | 2 | 2 | 190 | 3 | 191 | 1.07e-84 | 266 |
| MS.gene90337.t1 | MTR_7g060370 | 72.941 | 170 | 44 | 1 | 195 | 364 | 281 | 448 | 5.98e-84 | 264 |
| MS.gene90337.t1 | MTR_7g446270 | 70.466 | 193 | 50 | 4 | 1 | 189 | 1 | 190 | 3.99e-82 | 259 |
| MS.gene90337.t1 | MTR_7g446270 | 59.770 | 174 | 67 | 2 | 195 | 365 | 290 | 463 | 1.22e-65 | 216 |
| MS.gene90337.t1 | MTR_7g059540 | 68.718 | 195 | 56 | 4 | 1 | 191 | 2 | 195 | 7.94e-78 | 248 |
| MS.gene90337.t1 | MTR_7g059540 | 60.795 | 176 | 68 | 1 | 205 | 379 | 284 | 459 | 6.68e-67 | 219 |
| MS.gene90337.t1 | MTR_7g059540 | 68.718 | 195 | 56 | 4 | 1 | 191 | 1 | 194 | 7.94e-78 | 248 |
| MS.gene90337.t1 | MTR_7g059540 | 60.795 | 176 | 68 | 1 | 205 | 379 | 293 | 468 | 5.04e-67 | 220 |
| MS.gene90337.t1 | MTR_7g060080 | 64.407 | 177 | 62 | 1 | 200 | 375 | 4 | 180 | 1.19e-74 | 230 |
| MS.gene90337.t1 | MTR_7g059525 | 62.921 | 178 | 65 | 1 | 203 | 379 | 99 | 276 | 1.86e-72 | 228 |
| MS.gene90337.t1 | MTR_8g042170 | 34.858 | 459 | 205 | 11 | 6 | 379 | 1 | 450 | 7.93e-72 | 232 |
| MS.gene90337.t1 | MTR_7g060330 | 61.538 | 195 | 63 | 4 | 1 | 191 | 2 | 188 | 1.10e-69 | 227 |
| MS.gene90337.t1 | MTR_7g060330 | 57.485 | 167 | 67 | 3 | 200 | 365 | 303 | 466 | 1.76e-55 | 190 |
| MS.gene90337.t1 | MTR_7g059510 | 51.389 | 216 | 93 | 4 | 168 | 379 | 203 | 410 | 1.09e-62 | 207 |
| MS.gene90337.t1 | MTR_7g059510 | 51.705 | 176 | 41 | 3 | 5 | 177 | 6 | 140 | 2.41e-46 | 164 |
| MS.gene90337.t1 | MTR_7g059520 | 57.558 | 172 | 64 | 2 | 209 | 379 | 2 | 165 | 2.17e-62 | 198 |
| MS.gene90337.t1 | MTR_7g060060 | 70.629 | 143 | 41 | 1 | 49 | 191 | 1 | 142 | 3.93e-59 | 191 |
| MS.gene90337.t1 | MTR_7g059475 | 73.333 | 120 | 31 | 1 | 70 | 189 | 1 | 119 | 3.19e-50 | 169 |
| MS.gene90337.t1 | MTR_8g037920 | 31.931 | 404 | 193 | 11 | 49 | 379 | 8 | 402 | 1.09e-49 | 172 |
| MS.gene90337.t1 | MTR_1g048070 | 50.829 | 181 | 63 | 5 | 2 | 180 | 3 | 159 | 1.16e-46 | 160 |
| MS.gene90337.t1 | MTR_8g042400 | 32.047 | 337 | 160 | 6 | 110 | 379 | 3 | 337 | 5.60e-42 | 150 |
| MS.gene90337.t1 | MTR_7g060200 | 69.444 | 108 | 30 | 2 | 70 | 177 | 1 | 105 | 1.50e-40 | 142 |
| MS.gene90337.t1 | MTR_8g037923 | 32.979 | 282 | 157 | 6 | 115 | 379 | 197 | 463 | 5.27e-36 | 137 |
| MS.gene90337.t1 | MTR_8g037923 | 40.526 | 190 | 93 | 4 | 1 | 179 | 1 | 181 | 4.01e-35 | 135 |
| MS.gene90337.t1 | MTR_8g042070 | 39.412 | 170 | 87 | 3 | 210 | 363 | 71 | 240 | 4.34e-33 | 125 |
| MS.gene90337.t1 | MTR_8g042080 | 41.011 | 178 | 85 | 5 | 6 | 172 | 1 | 169 | 6.35e-32 | 119 |
| MS.gene90337.t1 | MTR_5g016570 | 40.252 | 159 | 83 | 6 | 214 | 367 | 356 | 507 | 3.53e-22 | 98.6 |
| MS.gene90337.t1 | MTR_8g005235 | 26.250 | 400 | 219 | 13 | 41 | 375 | 41 | 429 | 5.91e-22 | 97.4 |
| MS.gene90337.t1 | MTR_5g086340 | 25.424 | 413 | 226 | 15 | 41 | 389 | 46 | 440 | 8.31e-21 | 94.0 |
| MS.gene90337.t1 | MTR_8g005225 | 23.210 | 461 | 262 | 16 | 2 | 385 | 14 | 459 | 2.83e-20 | 92.4 |
| MS.gene90337.t1 | MTR_8g005325 | 25.990 | 404 | 192 | 12 | 41 | 363 | 70 | 447 | 1.21e-19 | 90.9 |
| MS.gene90337.t1 | MTR_5g086260 | 33.742 | 163 | 102 | 3 | 212 | 372 | 136 | 294 | 1.28e-19 | 89.0 |
| MS.gene90337.t1 | MTR_8g006525 | 27.446 | 419 | 206 | 15 | 8 | 354 | 11 | 403 | 2.71e-19 | 89.4 |
| MS.gene90337.t1 | MTR_6g009590 | 25.459 | 381 | 218 | 14 | 37 | 371 | 35 | 395 | 2.62e-17 | 83.6 |
| MS.gene90337.t1 | MTR_8g005265 | 25.000 | 396 | 221 | 15 | 41 | 365 | 41 | 431 | 5.90e-17 | 82.4 |
| MS.gene90337.t1 | MTR_5g087580 | 30.508 | 177 | 116 | 4 | 197 | 371 | 28 | 199 | 1.09e-16 | 79.0 |
| MS.gene90337.t1 | MTR_0209s0060 | 25.126 | 398 | 214 | 14 | 44 | 375 | 46 | 425 | 3.28e-16 | 80.1 |
| MS.gene90337.t1 | MTR_0209s0030 | 25.126 | 398 | 214 | 14 | 44 | 375 | 46 | 425 | 3.28e-16 | 80.1 |
| MS.gene90337.t1 | MTR_8g005295 | 37.500 | 136 | 77 | 3 | 242 | 370 | 364 | 498 | 6.43e-15 | 76.6 |
| MS.gene90337.t1 | MTR_6g011050 | 23.239 | 426 | 239 | 16 | 1 | 371 | 1 | 393 | 1.55e-14 | 75.1 |
| MS.gene90337.t1 | MTR_7g060105 | 66.038 | 53 | 17 | 1 | 286 | 337 | 1 | 53 | 1.92e-14 | 71.2 |
| MS.gene90337.t1 | MTR_8g042090 | 53.125 | 64 | 29 | 1 | 263 | 325 | 9 | 72 | 2.43e-14 | 69.3 |
| MS.gene90337.t1 | MTR_8g005285 | 36.765 | 136 | 78 | 3 | 242 | 370 | 350 | 484 | 3.78e-14 | 74.3 |
| MS.gene90337.t1 | MTR_6g009170 | 25.858 | 379 | 193 | 18 | 37 | 363 | 35 | 377 | 1.25e-13 | 72.4 |
| MS.gene90337.t1 | MTR_3g079390 | 31.288 | 163 | 103 | 6 | 217 | 375 | 281 | 438 | 9.74e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90337.t1 | AT3G50120 | 27.586 | 435 | 210 | 16 | 27 | 379 | 105 | 516 | 9.34e-28 | 115 |
| MS.gene90337.t1 | AT3G50180 | 25.688 | 436 | 243 | 14 | 5 | 379 | 158 | 573 | 9.97e-25 | 106 |
| MS.gene90337.t1 | AT5G22540 | 26.455 | 378 | 208 | 12 | 42 | 364 | 46 | 408 | 5.96e-23 | 100 |
| MS.gene90337.t1 | AT5G11290 | 28.232 | 379 | 205 | 15 | 44 | 375 | 50 | 408 | 3.49e-22 | 97.8 |
| MS.gene90337.t1 | AT3G50190 | 27.723 | 404 | 210 | 19 | 44 | 389 | 113 | 492 | 5.06e-22 | 97.8 |
| MS.gene90337.t1 | AT3G50160 | 26.005 | 423 | 223 | 13 | 27 | 388 | 106 | 499 | 3.27e-21 | 95.5 |
| MS.gene90337.t1 | AT3G47200 | 24.631 | 406 | 225 | 13 | 42 | 381 | 61 | 451 | 3.44e-19 | 89.4 |
| MS.gene90337.t1 | AT3G47200 | 24.631 | 406 | 225 | 13 | 42 | 381 | 61 | 451 | 3.44e-19 | 89.4 |
| MS.gene90337.t1 | AT3G50170 | 34.426 | 183 | 106 | 6 | 214 | 388 | 361 | 537 | 1.34e-18 | 87.8 |
| MS.gene90337.t1 | AT3G50130 | 36.250 | 160 | 92 | 5 | 214 | 369 | 386 | 539 | 7.51e-18 | 85.9 |
| MS.gene90337.t1 | AT3G50140 | 32.973 | 185 | 109 | 6 | 214 | 389 | 361 | 539 | 1.38e-17 | 84.7 |
| MS.gene90337.t1 | AT2G36430 | 25.789 | 380 | 202 | 16 | 44 | 364 | 62 | 420 | 1.95e-17 | 84.0 |
| MS.gene90337.t1 | AT3G50150 | 33.526 | 173 | 104 | 6 | 214 | 381 | 331 | 497 | 1.19e-16 | 82.0 |
| MS.gene90337.t1 | AT5G11290 | 27.374 | 358 | 193 | 15 | 65 | 375 | 2 | 339 | 5.60e-15 | 75.9 |
| MS.gene90337.t1 | AT1G67150 | 25.879 | 398 | 228 | 19 | 25 | 371 | 6 | 387 | 8.64e-12 | 66.6 |
| MS.gene90337.t1 | AT1G67150 | 25.879 | 398 | 228 | 19 | 25 | 371 | 6 | 387 | 8.64e-12 | 66.6 |
| MS.gene90337.t1 | AT3G44710 | 29.508 | 183 | 106 | 7 | 215 | 380 | 325 | 501 | 9.12e-12 | 67.0 |
| MS.gene90337.t1 | AT5G22550 | 32.530 | 166 | 101 | 7 | 215 | 375 | 312 | 471 | 3.87e-11 | 65.1 |
| MS.gene90337.t1 | AT5G22550 | 32.530 | 166 | 101 | 7 | 215 | 375 | 263 | 422 | 4.12e-11 | 64.7 |
| MS.gene90337.t1 | AT1G67150 | 25.564 | 399 | 232 | 19 | 25 | 371 | 6 | 391 | 4.58e-11 | 64.3 |
Find 71 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTTTGGTCGTGCATTTC+GGG | 0.191804 | 7.4:+43397270 | None:intergenic |
| TTTATGCCCCTATATGTAAT+TGG | 0.218395 | 7.4:+43396707 | None:intergenic |
| TCTGCCACCCTTTGTATCTT+TGG | 0.256920 | 7.4:+43397256 | None:intergenic |
| TCCTCGCCTGTCTTCAGATT+TGG | 0.275882 | 7.4:+43397154 | None:intergenic |
| ATTCAAGCCAATTACATATA+GGG | 0.304298 | 7.4:-43396714 | MS.gene90337:CDS |
| CTCAGTACCTTCACATCTTC+AGG | 0.311435 | 7.4:+43396452 | None:intergenic |
| AAGCCAAGCTTGGTGAACTA+TGG | 0.326118 | 7.4:-43396907 | MS.gene90337:CDS |
| GTATCTTTGGTCGTGCATTT+CGG | 0.327768 | 7.4:+43397269 | None:intergenic |
| GCTTGGCTTTCTCCAAGATA+TGG | 0.344706 | 7.4:+43396921 | None:intergenic |
| TGTTCGTGGATGGATGTTCT+TGG | 0.360549 | 7.4:-43396946 | MS.gene90337:CDS |
| AATTCAAGCCAATTACATAT+AGG | 0.367376 | 7.4:-43396715 | MS.gene90337:CDS |
| TCACCCAAGTTTGTGTCATT+AGG | 0.378033 | 7.4:-43397196 | MS.gene90337:CDS |
| GACACATCTCATATGCTATC+AGG | 0.397592 | 7.4:+43396540 | None:intergenic |
| GTGAAGGTACTGAGATCAAA+AGG | 0.406085 | 7.4:-43396443 | MS.gene90337:CDS |
| ACTATGGGACATGAATATTA+AGG | 0.413137 | 7.4:-43396891 | MS.gene90337:CDS |
| GGAAAATCTCTTGAAGGCTT+TGG | 0.414375 | 7.4:-43396998 | MS.gene90337:CDS |
| ATGTTCTTGGCTCCATATCT+TGG | 0.418956 | 7.4:-43396933 | MS.gene90337:CDS |
| AAGCAGCAGGAATAAAACTT+AGG | 0.425861 | 7.4:-43396676 | MS.gene90337:CDS |
| AGCATTACTTTAATAAATAC+AGG | 0.430752 | 7.4:-43396307 | MS.gene90337:CDS |
| TCAGATTTGGATTGTCATGA+TGG | 0.442609 | 7.4:+43397167 | None:intergenic |
| TTGTTCTTATGTAGCTCTCA+TGG | 0.446681 | 7.4:-43396492 | MS.gene90337:CDS |
| CTCTTACTTTCATTCAGACA+TGG | 0.453570 | 7.4:-43396201 | MS.gene90337:CDS |
| TCAACCGGAAAATCTCTTGA+AGG | 0.459462 | 7.4:-43397004 | MS.gene90337:CDS |
| ATGAAGTACTCAAGCTATTG+AGG | 0.473350 | 7.4:-43396811 | MS.gene90337:CDS |
| AACTTCCTCATCACTCCAAA+AGG | 0.479306 | 7.4:+43396409 | None:intergenic |
| GTCCCATAGTTCACCAAGCT+TGG | 0.480386 | 7.4:+43396904 | None:intergenic |
| AGCAGCAGGAATAAAACTTA+GGG | 0.482066 | 7.4:-43396675 | MS.gene90337:CDS |
| AATAGCTTGAGTACTTCATA+AGG | 0.488338 | 7.4:+43396815 | None:intergenic |
| ATCAATTTGTTCTTGTGATG+AGG | 0.493885 | 7.4:-43396865 | MS.gene90337:CDS |
| AGGACCTAATGACACAAACT+TGG | 0.499695 | 7.4:+43397192 | None:intergenic |
| ACTTTAATAAATACAGGACT+TGG | 0.506545 | 7.4:-43396301 | MS.gene90337:CDS |
| TGCAGCAAGGAATGCAATAA+CGG | 0.510768 | 7.4:+43396238 | None:intergenic |
| TCGTGGATGTTGTTCGTGGA+TGG | 0.513851 | 7.4:-43396956 | MS.gene90337:CDS |
| TGAATGAAAGTAAGAGCAAA+GGG | 0.516052 | 7.4:+43396208 | None:intergenic |
| ACCTATGATATTGAAAAGAC+TGG | 0.521468 | 7.4:+43396385 | None:intergenic |
| CAATATCATAGGTACTGACT+TGG | 0.522571 | 7.4:-43396375 | MS.gene90337:CDS |
| ACCACCTTTATTCGCTAGCC+GGG | 0.523605 | 7.4:+43396167 | None:intergenic |
| ATACAGGACTTGGATAGCGC+TGG | 0.525922 | 7.4:-43396291 | MS.gene90337:CDS |
| TTTGGATTGTCATGATGGAT+AGG | 0.526326 | 7.4:+43397172 | None:intergenic |
| AATGCTCACGTAATTTCAGC+TGG | 0.528143 | 7.4:+43396324 | None:intergenic |
| ATAAAAGATCTAAAAGCAGC+AGG | 0.528200 | 7.4:-43396689 | MS.gene90337:CDS |
| ATGAAGCTAAAATCAGAGAA+TGG | 0.532341 | 7.4:-43396754 | MS.gene90337:CDS |
| TCAGAGAAAACTATGTCTGT+TGG | 0.532878 | 7.4:+43396635 | None:intergenic |
| GACAATCCAAATCTGAAGAC+AGG | 0.533749 | 7.4:-43397160 | MS.gene90337:CDS |
| AACCACCTTTATTCGCTAGC+CGG | 0.537411 | 7.4:+43396166 | None:intergenic |
| AGCCAAGCTTGGTGAACTAT+GGG | 0.539042 | 7.4:-43396906 | MS.gene90337:CDS |
| TTCAAGCCAATTACATATAG+GGG | 0.541529 | 7.4:-43396713 | MS.gene90337:CDS |
| TACAGGACTTGGATAGCGCT+GGG | 0.544301 | 7.4:-43396290 | MS.gene90337:CDS |
| ATCTTGGAGAAAGCCAAGCT+TGG | 0.551611 | 7.4:-43396917 | MS.gene90337:CDS |
| TGCACGACCAAAGATACAAA+GGG | 0.554648 | 7.4:-43397263 | MS.gene90337:CDS |
| TCAATTTGTTCTTGTGATGA+GGG | 0.556081 | 7.4:-43396864 | MS.gene90337:CDS |
| GACATGGTTTACAGTTCACC+CGG | 0.563884 | 7.4:-43396185 | MS.gene90337:CDS |
| GCGAGGAGTACAAGCTTGTG+TGG | 0.567962 | 7.4:-43397138 | MS.gene90337:CDS |
| AGGAATGCAATAACGGTCCA+AGG | 0.588287 | 7.4:+43396245 | None:intergenic |
| TGATAACATCGATGAACTGA+AGG | 0.593398 | 7.4:-43397053 | MS.gene90337:CDS |
| ATCTTTGGTCGTGCATTTCG+GGG | 0.595449 | 7.4:+43397271 | None:intergenic |
| TGAAGGTACTGAGATCAAAA+GGG | 0.595602 | 7.4:-43396442 | MS.gene90337:CDS |
| CTGAATGAAAGTAAGAGCAA+AGG | 0.600280 | 7.4:+43396207 | None:intergenic |
| CGACCATCCTGAAGATGTGA+AGG | 0.601241 | 7.4:-43396459 | MS.gene90337:CDS |
| ATGCACGACCAAAGATACAA+AGG | 0.613476 | 7.4:-43397264 | MS.gene90337:CDS |
| GCTATCGTGGATGTTGTTCG+TGG | 0.621293 | 7.4:-43396960 | MS.gene90337:CDS |
| CGAGGAGTACAAGCTTGTGT+GGG | 0.637890 | 7.4:-43397137 | MS.gene90337:CDS |
| GGACCTAATGACACAAACTT+GGG | 0.637915 | 7.4:+43397193 | None:intergenic |
| GCCTTGAAGAAAAGCTATCG+TGG | 0.641023 | 7.4:-43396973 | MS.gene90337:CDS |
| TATCAGGTTGAGTAAAGTAG+AGG | 0.648563 | 7.4:+43396556 | None:intergenic |
| AAATACATAGAAGAAAACAC+TGG | 0.656917 | 7.4:-43397109 | MS.gene90337:CDS |
| GTACCTTCACATCTTCAGGA+TGG | 0.667216 | 7.4:+43396456 | None:intergenic |
| AAAGGGCAATAAATGCAGCA+AGG | 0.676037 | 7.4:+43396225 | None:intergenic |
| TCCAAATCTGAAGACAGGCG+AGG | 0.711316 | 7.4:-43397155 | MS.gene90337:CDS |
| AAACACTGGACTTATGCCAG+AGG | 0.728635 | 7.4:-43397095 | MS.gene90337:CDS |
| ACGACCAAAGATACAAAGGG+TGG | 0.740873 | 7.4:-43397260 | MS.gene90337:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTATTAAAGAAAAAATTG+TGG | - | chr7.4:43396192-43396211 | MS.gene90337:CDS | 15.0% |
| !!! | AATTTTTTCTTTAATAGTTG+GGG | + | chr7.4:43396191-43396210 | None:intergenic | 15.0% |
| !!! | ACAATTTTTTCTTTAATAGT+TGG | + | chr7.4:43396193-43396212 | None:intergenic | 15.0% |
| !!! | CAATTTTTTCTTTAATAGTT+GGG | + | chr7.4:43396192-43396211 | None:intergenic | 15.0% |
| !! | AGCATTACTTTAATAAATAC+AGG | - | chr7.4:43397202-43397221 | MS.gene90337:CDS | 20.0% |
| ! | AAATACATAGAAGAAAACAC+TGG | - | chr7.4:43396400-43396419 | MS.gene90337:CDS | 25.0% |
| ! | AATTCAAGCCAATTACATAT+AGG | - | chr7.4:43396794-43396813 | MS.gene90337:CDS | 25.0% |
| ! | ACTTTAATAAATACAGGACT+TGG | - | chr7.4:43397208-43397227 | MS.gene90337:CDS | 25.0% |
| ! | ATTCAAGCCAATTACATATA+GGG | - | chr7.4:43396795-43396814 | MS.gene90337:CDS | 25.0% |
| !!! | AAGTTGTTCACATTGATTTT+AGG | + | chr7.4:43396928-43396947 | None:intergenic | 25.0% |
| AATAGCTTGAGTACTTCATA+AGG | + | chr7.4:43396697-43396716 | None:intergenic | 30.0% | |
| ACCTATGATATTGAAAAGAC+TGG | + | chr7.4:43397127-43397146 | None:intergenic | 30.0% | |
| ACTATGGGACATGAATATTA+AGG | - | chr7.4:43396618-43396637 | MS.gene90337:CDS | 30.0% | |
| ATAAAAGATCTAAAAGCAGC+AGG | - | chr7.4:43396820-43396839 | MS.gene90337:CDS | 30.0% | |
| ATGAAGCTAAAATCAGAGAA+TGG | - | chr7.4:43396755-43396774 | MS.gene90337:CDS | 30.0% | |
| TGAATGAAAGTAAGAGCAAA+GGG | + | chr7.4:43397304-43397323 | None:intergenic | 30.0% | |
| TTCAAGCCAATTACATATAG+GGG | - | chr7.4:43396796-43396815 | MS.gene90337:CDS | 30.0% | |
| TTTATGCCCCTATATGTAAT+TGG | + | chr7.4:43396805-43396824 | None:intergenic | 30.0% | |
| ! | ATCAATTTGTTCTTGTGATG+AGG | - | chr7.4:43396644-43396663 | MS.gene90337:CDS | 30.0% |
| ! | TCAATTTGTTCTTGTGATGA+GGG | - | chr7.4:43396645-43396664 | MS.gene90337:CDS | 30.0% |
| AAGCAGCAGGAATAAAACTT+AGG | - | chr7.4:43396833-43396852 | MS.gene90337:CDS | 35.0% | |
| AGCAGCAGGAATAAAACTTA+GGG | - | chr7.4:43396834-43396853 | MS.gene90337:CDS | 35.0% | |
| CTCTTACTTTCATTCAGACA+TGG | - | chr7.4:43397308-43397327 | MS.gene90337:CDS | 35.0% | |
| CTGAATGAAAGTAAGAGCAA+AGG | + | chr7.4:43397305-43397324 | None:intergenic | 35.0% | |
| GCACTTACTTCAAAAATCCT+TGG | - | chr7.4:43397247-43397266 | MS.gene90337:CDS | 35.0% | |
| TCAGAGAAAACTATGTCTGT+TGG | + | chr7.4:43396877-43396896 | None:intergenic | 35.0% | |
| TCAGATTTGGATTGTCATGA+TGG | + | chr7.4:43396345-43396364 | None:intergenic | 35.0% | |
| TGATAACATCGATGAACTGA+AGG | - | chr7.4:43396456-43396475 | MS.gene90337:CDS | 35.0% | |
| TTGTTCTTATGTAGCTCTCA+TGG | - | chr7.4:43397017-43397036 | MS.gene90337:CDS | 35.0% | |
| ! | AAAGCCTTCAAGAGATTTTC+CGG | + | chr7.4:43396512-43396531 | None:intergenic | 35.0% |
| ! | ACAGACATAGTTTTCTCTGA+AGG | - | chr7.4:43396877-43396896 | MS.gene90337:CDS | 35.0% |
| ! | ATCTTTTTGTGTAGTTCCTC+TGG | + | chr7.4:43396433-43396452 | None:intergenic | 35.0% |
| ! | ATGAAGTACTCAAGCTATTG+AGG | - | chr7.4:43396698-43396717 | MS.gene90337:CDS | 35.0% |
| ! | GCCAGTCTTTTCAATATCAT+AGG | - | chr7.4:43397123-43397142 | MS.gene90337:CDS | 35.0% |
| !! | ACATAGTTTTCTCTGAAGGA+TGG | - | chr7.4:43396881-43396900 | MS.gene90337:CDS | 35.0% |
| !! | CAATATCATAGGTACTGACT+TGG | - | chr7.4:43397134-43397153 | MS.gene90337:CDS | 35.0% |
| !! | TATCAGGTTGAGTAAAGTAG+AGG | + | chr7.4:43396956-43396975 | None:intergenic | 35.0% |
| !! | TGAAGGTACTGAGATCAAAA+GGG | - | chr7.4:43397067-43397086 | MS.gene90337:CDS | 35.0% |
| !! | TTTGGATTGTCATGATGGAT+AGG | + | chr7.4:43396340-43396359 | None:intergenic | 35.0% |
| !!! | GACGATGTTTTAACTTCAAC+CGG | - | chr7.4:43396490-43396509 | MS.gene90337:CDS | 35.0% |
| AAAGGGCAATAAATGCAGCA+AGG | + | chr7.4:43397287-43397306 | None:intergenic | 40.0% | |
| AACTTCCTCATCACTCCAAA+AGG | + | chr7.4:43397103-43397122 | None:intergenic | 40.0% | |
| AATGCTCACGTAATTTCAGC+TGG | + | chr7.4:43397188-43397207 | None:intergenic | 40.0% | |
| AGGACCTAATGACACAAACT+TGG | + | chr7.4:43396320-43396339 | None:intergenic | 40.0% | |
| ATGCACGACCAAAGATACAA+AGG | - | chr7.4:43396245-43396264 | MS.gene90337:CDS | 40.0% | |
| ATGTTCTTGGCTCCATATCT+TGG | - | chr7.4:43396576-43396595 | MS.gene90337:CDS | 40.0% | |
| GACAATCCAAATCTGAAGAC+AGG | - | chr7.4:43396349-43396368 | MS.gene90337:CDS | 40.0% | |
| GACACATCTCATATGCTATC+AGG | + | chr7.4:43396972-43396991 | None:intergenic | 40.0% | |
| GGAAAATCTCTTGAAGGCTT+TGG | - | chr7.4:43396511-43396530 | MS.gene90337:CDS | 40.0% | |
| GGACCTAATGACACAAACTT+GGG | + | chr7.4:43396319-43396338 | None:intergenic | 40.0% | |
| GTATCTTTGGTCGTGCATTT+CGG | + | chr7.4:43396243-43396262 | None:intergenic | 40.0% | |
| TATCTTTGGTCGTGCATTTC+GGG | + | chr7.4:43396242-43396261 | None:intergenic | 40.0% | |
| TCAACCGGAAAATCTCTTGA+AGG | - | chr7.4:43396505-43396524 | MS.gene90337:CDS | 40.0% | |
| TGCACGACCAAAGATACAAA+GGG | - | chr7.4:43396246-43396265 | MS.gene90337:CDS | 40.0% | |
| TGCAGCAAGGAATGCAATAA+CGG | + | chr7.4:43397274-43397293 | None:intergenic | 40.0% | |
| ! | AAGGGATTTTGTGCACCTTT+TGG | - | chr7.4:43397085-43397104 | MS.gene90337:CDS | 40.0% |
| ! | TCACCCAAGTTTGTGTCATT+AGG | - | chr7.4:43396313-43396332 | MS.gene90337:CDS | 40.0% |
| ! | TCCACGATAGCTTTTCTTCA+AGG | + | chr7.4:43396540-43396559 | None:intergenic | 40.0% |
| !! | GTGAAGGTACTGAGATCAAA+AGG | - | chr7.4:43397066-43397085 | MS.gene90337:CDS | 40.0% |
| AAACACTGGACTTATGCCAG+AGG | - | chr7.4:43396414-43396433 | MS.gene90337:CDS | 45.0% | |
| ACGACCAAAGATACAAAGGG+TGG | - | chr7.4:43396249-43396268 | MS.gene90337:CDS | 45.0% | |
| AGGAATGCAATAACGGTCCA+AGG | + | chr7.4:43397267-43397286 | None:intergenic | 45.0% | |
| ATCTTGGAGAAAGCCAAGCT+TGG | - | chr7.4:43396592-43396611 | MS.gene90337:CDS | 45.0% | |
| ATCTTTGGTCGTGCATTTCG+GGG | + | chr7.4:43396241-43396260 | None:intergenic | 45.0% | |
| CTCAGTACCTTCACATCTTC+AGG | + | chr7.4:43397060-43397079 | None:intergenic | 45.0% | |
| GACATGGTTTACAGTTCACC+CGG | - | chr7.4:43397324-43397343 | MS.gene90337:CDS | 45.0% | |
| GCCTTGAAGAAAAGCTATCG+TGG | - | chr7.4:43396536-43396555 | MS.gene90337:CDS | 45.0% | |
| GTACCTTCACATCTTCAGGA+TGG | + | chr7.4:43397056-43397075 | None:intergenic | 45.0% | |
| TCTGCCACCCTTTGTATCTT+TGG | + | chr7.4:43396256-43396275 | None:intergenic | 45.0% | |
| TGTTCGTGGATGGATGTTCT+TGG | - | chr7.4:43396563-43396582 | MS.gene90337:CDS | 45.0% | |
| ! | AAGCCAAGCTTGGTGAACTA+TGG | - | chr7.4:43396602-43396621 | MS.gene90337:CDS | 45.0% |
| ! | AGCCAAGCTTGGTGAACTAT+GGG | - | chr7.4:43396603-43396622 | MS.gene90337:CDS | 45.0% |
| ! | GATGAGGGATGTGCTTTTGT+TGG | - | chr7.4:43396660-43396679 | MS.gene90337:CDS | 45.0% |
| ! | GCTTGGCTTTCTCCAAGATA+TGG | + | chr7.4:43396591-43396610 | None:intergenic | 45.0% |
| ATACAGGACTTGGATAGCGC+TGG | - | chr7.4:43397218-43397237 | MS.gene90337:CDS | 50.0% | |
| CGACCATCCTGAAGATGTGA+AGG | - | chr7.4:43397050-43397069 | MS.gene90337:CDS | 50.0% | |
| GCTATCGTGGATGTTGTTCG+TGG | - | chr7.4:43396549-43396568 | MS.gene90337:CDS | 50.0% | |
| GTCCCATAGTTCACCAAGCT+TGG | + | chr7.4:43396608-43396627 | None:intergenic | 50.0% | |
| TACAGGACTTGGATAGCGCT+GGG | - | chr7.4:43397219-43397238 | MS.gene90337:CDS | 50.0% | |
| TCCAAATCTGAAGACAGGCG+AGG | - | chr7.4:43396354-43396373 | MS.gene90337:CDS | 50.0% | |
| TCCTCGCCTGTCTTCAGATT+TGG | + | chr7.4:43396358-43396377 | None:intergenic | 50.0% | |
| TCGTGGATGTTGTTCGTGGA+TGG | - | chr7.4:43396553-43396572 | MS.gene90337:CDS | 50.0% | |
| ! | CGAGGAGTACAAGCTTGTGT+GGG | - | chr7.4:43396372-43396391 | MS.gene90337:CDS | 50.0% |
| !!! | GTGCACCTTTTGGAGTGATG+AGG | - | chr7.4:43397095-43397114 | MS.gene90337:CDS | 50.0% |
| ! | GCGAGGAGTACAAGCTTGTG+TGG | - | chr7.4:43396371-43396390 | MS.gene90337:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 43396181 | 43397350 | 43396181 | ID=MS.gene90337 |
| chr7.4 | mRNA | 43396181 | 43397350 | 43396181 | ID=MS.gene90337.t1;Parent=MS.gene90337 |
| chr7.4 | exon | 43396181 | 43397350 | 43396181 | ID=MS.gene90337.t1.exon1;Parent=MS.gene90337.t1 |
| chr7.4 | CDS | 43396181 | 43397350 | 43396181 | ID=cds.MS.gene90337.t1;Parent=MS.gene90337.t1 |
| Gene Sequence |
| Protein sequence |
