AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90347


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90347.t1 MTR_4g083510 85.455 330 39 1 1 330 1 321 0.0 555
MS.gene90347.t1 MTR_0867s0010 77.812 329 63 2 1 329 1 319 7.91e-174 485
MS.gene90347.t1 MTR_4g063670 71.515 330 84 2 1 330 1 320 1.20e-163 459
MS.gene90347.t1 MTR_1g051185 61.794 301 80 2 1 301 1 266 5.28e-117 338
MS.gene90347.t1 MTR_8g015750 69.458 203 45 2 127 329 1 186 6.81e-85 254
MS.gene90347.t1 MTR_3g069460 62.842 183 59 1 1 183 1 174 5.58e-78 236
MS.gene90347.t1 MTR_7g088160 40.964 332 156 11 20 329 75 388 1.93e-60 198
MS.gene90347.t1 MTR_7g107340 40.769 260 123 10 78 329 98 334 7.57e-43 151
MS.gene90347.t1 MTR_8g015740 72.527 91 16 1 1 91 49 130 1.86e-36 128
MS.gene90347.t1 MTR_2g023560 79.032 62 13 0 55 116 57 118 1.42e-27 104
MS.gene90347.t1 MTR_7g114170 32.166 314 124 12 18 328 61 288 1.67e-22 95.9
MS.gene90347.t1 MTR_6g081150 37.179 156 56 5 79 232 40 155 1.41e-20 87.8
MS.gene90347.t1 MTR_5g044920 56.757 74 23 1 43 116 17 81 1.13e-17 77.4
MS.gene90347.t1 MTR_4g013220 26.178 191 134 4 20 208 53 238 2.12e-12 67.4
MS.gene90347.t1 MTR_5g066280 25.654 191 135 4 20 208 53 238 1.22e-11 65.1
MS.gene90347.t1 MTR_3g067785 25.654 191 135 4 20 208 53 238 1.29e-11 65.1
MS.gene90347.t1 MTR_4g026120 25.654 191 135 4 20 208 53 238 1.37e-11 65.1
MS.gene90347.t1 MTR_4g036435 25.654 191 135 4 20 208 53 238 1.37e-11 65.1
MS.gene90347.t1 MTR_2g072860 29.825 114 78 2 68 181 72 183 1.60e-11 64.7
MS.gene90347.t1 MTR_1g106885 25.238 210 137 5 20 227 114 305 1.75e-11 65.1
MS.gene90347.t1 MTR_2g022120 25.238 210 137 5 20 227 117 308 1.80e-11 65.1
MS.gene90347.t1 MTR_7g461350 27.966 118 83 2 64 181 136 251 2.22e-11 64.7
MS.gene90347.t1 MTR_6g016670 27.966 118 83 2 64 181 136 251 2.22e-11 64.7
MS.gene90347.t1 MTR_3g464310 27.966 118 83 2 64 181 136 251 2.22e-11 64.7
MS.gene90347.t1 MTR_3g464170 27.966 118 83 2 64 181 136 251 2.22e-11 64.7
MS.gene90347.t1 MTR_1g100375 27.966 118 83 2 64 181 136 251 2.22e-11 64.7
MS.gene90347.t1 MTR_6g051405 27.966 118 83 2 64 181 136 251 2.59e-11 64.3
MS.gene90347.t1 MTR_3g450670 27.966 118 83 2 64 181 132 247 2.60e-11 64.3
MS.gene90347.t1 MTR_1g471070 27.966 118 83 2 64 181 132 247 2.60e-11 64.3
MS.gene90347.t1 MTR_5g077245 26.590 173 120 4 38 208 124 291 2.62e-11 64.3
MS.gene90347.t1 MTR_6g092533 26.442 208 125 5 20 227 117 296 3.19e-11 64.3
MS.gene90347.t1 MTR_7g062380 26.012 173 121 4 38 208 6 173 4.42e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90347.t1 AT5G41220 38.974 195 101 6 65 245 219 409 9.02e-32 125
MS.gene90347.t1 AT5G41220 40.000 195 99 7 65 245 227 417 1.10e-31 124
MS.gene90347.t1 AT5G41220 38.974 195 101 6 65 245 277 467 1.27e-31 125
MS.gene90347.t1 AT5G41240 37.838 185 102 6 60 232 272 455 1.11e-27 113
MS.gene90347.t1 AT3G47680 36.041 197 112 7 30 223 23 208 3.07e-26 106
MS.gene90347.t1 AT1G66235 29.947 187 115 5 61 232 7 192 2.17e-17 81.3

Find 58 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCTCTTGATCATTTAAT+TGG 0.210422 7.4:+43880269 None:intergenic
CCAACAAACTTTGAAATAGA+TGG 0.279570 7.4:+43879932 None:intergenic
CTTTGATCATTCCCAACAAT+TGG 0.310977 7.4:+43880067 None:intergenic
TTCAGCTTCTGGAGCATATT+CGG 0.312536 7.4:-43879717 MS.gene90347:CDS
AGAACAAAGAATTCAGCTTC+TGG 0.340604 7.4:-43879728 MS.gene90347:CDS
TCTTTCAAAGCATCAAATTT+CGG 0.344398 7.4:+43879584 None:intergenic
CATCTATTTCAAAGTTTGTT+GGG 0.369114 7.4:-43879931 MS.gene90347:CDS
GATGAACCGAAATGGCTTGC+AGG 0.369514 7.4:-43879776 MS.gene90347:CDS
AGAAAACCAAAGGCACTTAA+AGG 0.372874 7.4:-43879977 MS.gene90347:CDS
CATGCATTCTCAAGTGTGAA+TGG 0.388314 7.4:+43879812 None:intergenic
ACACAACCTGCAAGCCATTT+CGG 0.388495 7.4:+43879770 None:intergenic
GGTGGATTGTATTCACTTGT+TGG 0.390206 7.4:+43879674 None:intergenic
TGCAAAGAGTTGAAAATGAT+AGG 0.394552 7.4:-43879457 MS.gene90347:CDS
ATTTCAAAGGATCCAATTGT+TGG 0.399370 7.4:-43880079 MS.gene90347:CDS
CATCGACCTTTAAGTGCCTT+TGG 0.399464 7.4:+43879971 None:intergenic
TCATAATCTTGTAGTTGTCT+TGG 0.400714 7.4:+43879377 None:intergenic
ATGAGAAGTTCATCCTCCTT+TGG 0.406782 7.4:+43880118 None:intergenic
ATCCGAGAGCGACAACATTT+CGG 0.416235 7.4:-43879870 MS.gene90347:CDS
GGCTTGGAAACAATTAATCT+TGG 0.423881 7.4:-43880220 MS.gene90347:CDS
CCATCTATTTCAAAGTTTGT+TGG 0.437206 7.4:-43879932 MS.gene90347:CDS
TATTCACTTGTTGGTGTTGG+TGG 0.444501 7.4:+43879683 None:intergenic
TTTCAAAGGATCCAATTGTT+GGG 0.450141 7.4:-43880078 MS.gene90347:CDS
ACCTTGCACTCAAGATGGCT+TGG 0.463183 7.4:-43880236 MS.gene90347:CDS
ATGGCTTGCAGGTTGTGTAT+TGG 0.465781 7.4:-43879765 MS.gene90347:CDS
TATTGAGAGATGAACCGAAA+TGG 0.474221 7.4:-43879784 MS.gene90347:CDS
TTGTATTCACTTGTTGGTGT+TGG 0.477883 7.4:+43879680 None:intergenic
AGCCGAAATGTTGTCGCTCT+CGG 0.477888 7.4:+43879868 None:intergenic
ACAACGTCCAATCGGTCAAA+AGG 0.486049 7.4:-43879651 MS.gene90347:CDS
TCGGTCAAAAGGCGGCAAAA+AGG 0.487566 7.4:-43879640 MS.gene90347:CDS
ATGAACTTCTCATTCAATCA+TGG 0.496152 7.4:-43880108 MS.gene90347:CDS
TCCAAGCCATCTTGAGTGCA+AGG 0.501328 7.4:+43880235 None:intergenic
CTTGAAAATTCTTGTCGCGA+TGG 0.504105 7.4:+43880002 None:intergenic
GCAAAGAGTTGAAAATGATA+GGG 0.522318 7.4:-43879456 MS.gene90347:CDS
ATTCACTTGTTGGTGTTGGT+GGG 0.524603 7.4:+43879684 None:intergenic
TGAATGGTTCACGTACATCT+TGG 0.533697 7.4:+43879828 None:intergenic
AATCCACCACAACGTCCAAT+CGG 0.536837 7.4:-43879659 MS.gene90347:CDS
CCACAACCTTGCACTCAAGA+TGG 0.538111 7.4:-43880241 MS.gene90347:CDS
CCATCTTGAGTGCAAGGTTG+TGG 0.539414 7.4:+43880241 None:intergenic
CATTCACACTTGAGAATGCA+TGG 0.543088 7.4:-43879811 MS.gene90347:CDS
ATCATGGCTCAATATTTCAA+AGG 0.547059 7.4:-43880092 MS.gene90347:CDS
GAAGTTCAGATGAATAATAG+TGG 0.553798 7.4:-43880295 MS.gene90347:CDS
ATTAATCTTGGTGAAGAAGT+CGG 0.569606 7.4:-43880208 MS.gene90347:CDS
CATCTGAACTTCATGAGACA+TGG 0.573227 7.4:+43880306 None:intergenic
GGCGTGATTAGAGATAAATA+TGG 0.592289 7.4:-43879344 MS.gene90347:CDS
GTTGTCGCTCTAAAGAAAAG+TGG 0.594037 7.4:-43879896 MS.gene90347:CDS
AATAAGATTTAAACCAAAGG+AGG 0.598325 7.4:-43880131 MS.gene90347:CDS
TGACCGATTGGACGTTGTGG+TGG 0.605293 7.4:+43879656 None:intergenic
CAAAGGCACTTAAAGGTCGA+TGG 0.612087 7.4:-43879970 MS.gene90347:CDS
GAGTTGAAAATGATAGGGAA+AGG 0.618261 7.4:-43879451 MS.gene90347:CDS
AACAAGATTTCAACCAAAGG+AGG 0.620863 7.4:-43880158 MS.gene90347:CDS
GAAAATAAGATTTAAACCAA+AGG 0.637971 7.4:-43880134 MS.gene90347:CDS
GAAAACAAGATTTCAACCAA+AGG 0.640671 7.4:-43880161 MS.gene90347:CDS
GAAGGGAAAAGAAAAGCTTG+TGG 0.644203 7.4:-43879618 MS.gene90347:CDS
TGAAGAAGTCGGAGCTACAT+CGG 0.648185 7.4:-43880197 MS.gene90347:CDS
TGATTTAGAAATACTCACAA+AGG 0.661251 7.4:-43879417 MS.gene90347:CDS
ACGTCCAATCGGTCAAAAGG+CGG 0.661921 7.4:-43879648 MS.gene90347:CDS
ATTGTTGGGAATGATCAAAG+AGG 0.674803 7.4:-43880064 MS.gene90347:CDS
CAAGATTATGAATTTCTGTG+TGG 0.676928 7.4:-43879365 MS.gene90347:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAATAAGATTTAAACCAA+AGG - chr7.4:43879504-43879523 MS.gene90347:CDS 20.0%
! AATAAGATTTAAACCAAAGG+AGG - chr7.4:43879507-43879526 MS.gene90347:CDS 25.0%
! CAATCTCTTGATCATTTAAT+TGG + chr7.4:43879372-43879391 None:intergenic 25.0%
! TGATTTAGAAATACTCACAA+AGG - chr7.4:43880221-43880240 MS.gene90347:CDS 25.0%
!! CATCTATTTCAAAGTTTGTT+GGG - chr7.4:43879707-43879726 MS.gene90347:CDS 25.0%
!! TCTTTCAAAGCATCAAATTT+CGG + chr7.4:43880057-43880076 None:intergenic 25.0%
!! TGAATTCTTTGTTCTTTTTG+CGG + chr7.4:43879905-43879924 None:intergenic 25.0%
AATGGAGATAGAGAGAAAAA+AGG - chr7.4:43880146-43880165 MS.gene90347:CDS 30.0%
ATCATGGCTCAATATTTCAA+AGG - chr7.4:43879546-43879565 MS.gene90347:CDS 30.0%
ATGAACTTCTCATTCAATCA+TGG - chr7.4:43879530-43879549 MS.gene90347:CDS 30.0%
ATTAATCTTGGTGAAGAAGT+CGG - chr7.4:43879430-43879449 MS.gene90347:CDS 30.0%
ATTTCAAAGGATCCAATTGT+TGG - chr7.4:43879559-43879578 MS.gene90347:CDS 30.0%
CAAGATTATGAATTTCTGTG+TGG - chr7.4:43880273-43880292 MS.gene90347:CDS 30.0%
CCATCTATTTCAAAGTTTGT+TGG - chr7.4:43879706-43879725 MS.gene90347:CDS 30.0%
GAAAACAAGATTTCAACCAA+AGG - chr7.4:43879477-43879496 MS.gene90347:CDS 30.0%
GAAGTTCAGATGAATAATAG+TGG - chr7.4:43879343-43879362 MS.gene90347:CDS 30.0%
GCAAAGAGTTGAAAATGATA+GGG - chr7.4:43880182-43880201 MS.gene90347:CDS 30.0%
TCATAATCTTGTAGTTGTCT+TGG + chr7.4:43880264-43880283 None:intergenic 30.0%
TGCAAAGAGTTGAAAATGAT+AGG - chr7.4:43880181-43880200 MS.gene90347:CDS 30.0%
TTTCAAAGGATCCAATTGTT+GGG - chr7.4:43879560-43879579 MS.gene90347:CDS 30.0%
! ATCAAAGAGGAGATAGTTTT+TGG - chr7.4:43879587-43879606 MS.gene90347:CDS 30.0%
! ATTTTCAACTCTTTGCAACT+CGG + chr7.4:43880178-43880197 None:intergenic 30.0%
!! CCAACAAACTTTGAAATAGA+TGG + chr7.4:43879709-43879728 None:intergenic 30.0%
!! TTTTCAAGAGAGAAAACCAA+AGG - chr7.4:43879651-43879670 MS.gene90347:CDS 30.0%
!!! ACGTTTTGAGAGTGAAAAAA+TGG - chr7.4:43880128-43880147 MS.gene90347:CDS 30.0%
AACAAGATTTCAACCAAAGG+AGG - chr7.4:43879480-43879499 MS.gene90347:CDS 35.0%
AGAACAAAGAATTCAGCTTC+TGG - chr7.4:43879910-43879929 MS.gene90347:CDS 35.0%
ATTGTTGGGAATGATCAAAG+AGG - chr7.4:43879574-43879593 MS.gene90347:CDS 35.0%
CTTTGATCATTCCCAACAAT+TGG + chr7.4:43879574-43879593 None:intergenic 35.0%
GAGTTGAAAATGATAGGGAA+AGG - chr7.4:43880187-43880206 MS.gene90347:CDS 35.0%
GGCGTGATTAGAGATAAATA+TGG - chr7.4:43880294-43880313 MS.gene90347:CDS 35.0%
GGCTTGGAAACAATTAATCT+TGG - chr7.4:43879418-43879437 MS.gene90347:CDS 35.0%
TATTGAGAGATGAACCGAAA+TGG - chr7.4:43879854-43879873 MS.gene90347:CDS 35.0%
! TTCTTTGTTCTTTTTGCGGA+CGG + chr7.4:43879901-43879920 None:intergenic 35.0%
! TTGTATTCACTTGTTGGTGT+TGG + chr7.4:43879961-43879980 None:intergenic 35.0%
!! AGAAAACCAAAGGCACTTAA+AGG - chr7.4:43879661-43879680 MS.gene90347:CDS 35.0%
!! GATAGTTTTTGGAAGAGGAT+TGG - chr7.4:43879598-43879617 MS.gene90347:CDS 35.0%
!!! AATCTTATTTTCGCCTCCTT+TGG + chr7.4:43879496-43879515 None:intergenic 35.0%
ATGAGAAGTTCATCCTCCTT+TGG + chr7.4:43879523-43879542 None:intergenic 40.0%
CATGCATTCTCAAGTGTGAA+TGG + chr7.4:43879829-43879848 None:intergenic 40.0%
CATTCACACTTGAGAATGCA+TGG - chr7.4:43879827-43879846 MS.gene90347:CDS 40.0%
CTTGAAAATTCTTGTCGCGA+TGG + chr7.4:43879639-43879658 None:intergenic 40.0%
GAAGGGAAAAGAAAAGCTTG+TGG - chr7.4:43880020-43880039 MS.gene90347:CDS 40.0%
TGAATGGTTCACGTACATCT+TGG + chr7.4:43879813-43879832 None:intergenic 40.0%
!! ATTCACTTGTTGGTGTTGGT+GGG + chr7.4:43879957-43879976 None:intergenic 40.0%
!! GAGGAGATAGTTTTTGGAAG+AGG - chr7.4:43879593-43879612 MS.gene90347:CDS 40.0%
!! GGTGGATTGTATTCACTTGT+TGG + chr7.4:43879967-43879986 None:intergenic 40.0%
!! GTTGTCGCTCTAAAGAAAAG+TGG - chr7.4:43879742-43879761 MS.gene90347:CDS 40.0%
!! TATTCACTTGTTGGTGTTGG+TGG + chr7.4:43879958-43879977 None:intergenic 40.0%
!! TTCAGCTTCTGGAGCATATT+CGG - chr7.4:43879921-43879940 MS.gene90347:CDS 40.0%
AATCCACCACAACGTCCAAT+CGG - chr7.4:43879979-43879998 MS.gene90347:CDS 45.0%
ACAACGTCCAATCGGTCAAA+AGG - chr7.4:43879987-43880006 MS.gene90347:CDS 45.0%
ACACAACCTGCAAGCCATTT+CGG + chr7.4:43879871-43879890 None:intergenic 45.0%
ATCCGAGAGCGACAACATTT+CGG - chr7.4:43879768-43879787 MS.gene90347:CDS 45.0%
CAAAAGGCGGCAAAAAGGAA+GGG - chr7.4:43880003-43880022 MS.gene90347:CDS 45.0%
CATCGACCTTTAAGTGCCTT+TGG + chr7.4:43879670-43879689 None:intergenic 45.0%
TCAAAAGGCGGCAAAAAGGA+AGG - chr7.4:43880002-43880021 MS.gene90347:CDS 45.0%
TGAAGAAGTCGGAGCTACAT+CGG - chr7.4:43879441-43879460 MS.gene90347:CDS 45.0%
! ATGGCTTGCAGGTTGTGTAT+TGG - chr7.4:43879873-43879892 MS.gene90347:CDS 45.0%
! TTTTGACCGATTGGACGTTG+TGG + chr7.4:43879988-43880007 None:intergenic 45.0%
!! CAAAGGCACTTAAAGGTCGA+TGG - chr7.4:43879668-43879687 MS.gene90347:CDS 45.0%
ACCTTGCACTCAAGATGGCT+TGG - chr7.4:43879402-43879421 MS.gene90347:CDS 50.0%
ACGTCCAATCGGTCAAAAGG+CGG - chr7.4:43879990-43880009 MS.gene90347:CDS 50.0%
AGCCGAAATGTTGTCGCTCT+CGG + chr7.4:43879773-43879792 None:intergenic 50.0%
CCACAACCTTGCACTCAAGA+TGG - chr7.4:43879397-43879416 MS.gene90347:CDS 50.0%
CCATCTTGAGTGCAAGGTTG+TGG + chr7.4:43879400-43879419 None:intergenic 50.0%
TCCAAGCCATCTTGAGTGCA+AGG + chr7.4:43879406-43879425 None:intergenic 50.0%
TCGGTCAAAAGGCGGCAAAA+AGG - chr7.4:43879998-43880017 MS.gene90347:CDS 50.0%
! GATGAACCGAAATGGCTTGC+AGG - chr7.4:43879862-43879881 MS.gene90347:CDS 50.0%
! TTTGCCGCCTTTTGACCGAT+TGG + chr7.4:43879997-43880016 None:intergenic 50.0%
TGACCGATTGGACGTTGTGG+TGG + chr7.4:43879985-43880004 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 43879334 43880326 43879334 ID=MS.gene90347
chr7.4 mRNA 43879334 43880326 43879334 ID=MS.gene90347.t1;Parent=MS.gene90347
chr7.4 exon 43879334 43880326 43879334 ID=MS.gene90347.t1.exon1;Parent=MS.gene90347.t1
chr7.4 CDS 43879334 43880326 43879334 ID=cds.MS.gene90347.t1;Parent=MS.gene90347.t1
Gene Sequence

>MS.gene90347

ATGTCTCATGAAGTTCAGATGAATAATAGTGGTGTCCAATTAAATGATCAAGAGATTGAAACACCACAACCTTGCACTCAAGATGGCTTGGAAACAATTAATCTTGGTGAAGAAGTCGGAGCTACATCGGTTGCGAATACTTCGAAAACAAGATTTCAACCAAAGGAGGCGAAAATAAGATTTAAACCAAAGGAGGATGAACTTCTCATTCAATCATGGCTCAATATTTCAAAGGATCCAATTGTTGGGAATGATCAAAGAGGAGATAGTTTTTGGAAGAGGATTGGTGAAGCTTACAACAACCATCGCGACAAGAATTTTCAAGAGAGAAAACCAAAGGCACTTAAAGGTCGATGGCACAAAAAAATCAATCCATCTATTTCAAAGTTTGTTGGGTGCTACAAAGATGTTGTCGCTCTAAAGAAAAGTGGTGCATCCGAGAGCGACAACATTTCGGCTGCAAAAGATATTTATTACCAAGATGTACGTGAACCATTCACACTTGAGAATGCATGGAAATTATTGAGAGATGAACCGAAATGGCTTGCAGGTTGTGTATTGGAACCGTCCGCAAAAAGAACAAAGAATTCAGCTTCTGGAGCATATTCGGCATCTTCTAACCCACCAACACCAACAAGTGAATACAATCCACCACAACGTCCAATCGGTCAAAAGGCGGCAAAAAGGAAGGGAAAAGAAAAGCTTGTGGAAATTTCTACTCCGAAATTTGATGCTTTGAAAGATGACTTCAACAAAAAATTTGAATTAATGTCAGAGTTTGCACGTGATTATACACGTTTTGAGAGTGAAAAAATGGAGATAGAGAGAAAAAAGGTTGATGCCGAGTTGCAAAGAGTTGAAAATGATAGGGAAAGGTCGAAGATCAATGATTTAGAAATACTCACAAAGGATACATCAAATATGAATCCAAGACAACTACAAGATTATGAATTTCTGTGTGGCGTGATTAGAGATAAATATGGACTTAATTGA

Protein sequence

>MS.gene90347.t1

MSHEVQMNNSGVQLNDQEIETPQPCTQDGLETINLGEEVGATSVANTSKTRFQPKEAKIRFKPKEDELLIQSWLNISKDPIVGNDQRGDSFWKRIGEAYNNHRDKNFQERKPKALKGRWHKKINPSISKFVGCYKDVVALKKSGASESDNISAAKDIYYQDVREPFTLENAWKLLRDEPKWLAGCVLEPSAKRTKNSASGAYSASSNPPTPTSEYNPPQRPIGQKAAKRKGKEKLVEISTPKFDALKDDFNKKFELMSEFARDYTRFESEKMEIERKKVDAELQRVENDRERSKINDLEILTKDTSNMNPRQLQDYEFLCGVIRDKYGLN