Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90712.t1 | XP_024628618.1 | 47.6 | 208 | 102 | 2 | 2 | 209 | 13 | 213 | 3.20E-45 | 191.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90712.t1 | Q2HUA4 | 43.6 | 211 | 108 | 3 | 1 | 210 | 142 | 342 | 1.6e-43 | 185.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050359 | MS.gene90712 | -0.807278 | 5.47E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene90712 | -0.817165 | 3.80E-52 | -1.69E-46 |
| MS.gene053058 | MS.gene90712 | -0.8202 | 7.78E-53 | -1.69E-46 |
| MS.gene053167 | MS.gene90712 | -0.80742 | 5.10E-50 | -1.69E-46 |
| MS.gene053895 | MS.gene90712 | 0.811101 | 8.28E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene90712 | -0.828934 | 6.82E-55 | -1.69E-46 |
| MS.gene057094 | MS.gene90712 | -0.809732 | 1.64E-50 | -1.69E-46 |
| MS.gene060563 | MS.gene90712 | -0.815677 | 8.18E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90712.t1 | MTR_2g041060 | 31.496 | 127 | 85 | 1 | 47 | 173 | 9 | 133 | 7.70e-14 | 67.8 |
| MS.gene90712.t1 | MTR_8g487680 | 40.789 | 76 | 45 | 0 | 66 | 141 | 5 | 80 | 1.99e-13 | 65.5 |
| MS.gene90712.t1 | MTR_1g059630 | 45.783 | 83 | 40 | 2 | 127 | 209 | 223 | 300 | 2.20e-13 | 68.2 |
| MS.gene90712.t1 | MTR_8g032080 | 37.069 | 116 | 63 | 3 | 6 | 117 | 207 | 316 | 8.60e-12 | 63.5 |
| MS.gene90712.t1 | MTR_7g091620 | 46.429 | 56 | 30 | 0 | 119 | 174 | 38 | 93 | 1.93e-11 | 58.9 |
| MS.gene90712.t1 | MTR_8g470880 | 24.757 | 206 | 149 | 3 | 4 | 207 | 73 | 274 | 2.52e-11 | 62.4 |
| MS.gene90712.t1 | MTR_7g034685 | 33.333 | 81 | 54 | 0 | 94 | 174 | 19 | 99 | 3.86e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGACATGACCTTATTAATT+AGG | 0.125021 | 1.4:+1667995 | None:intergenic |
| GAATTGCTGCAATCTGATTT+TGG | 0.130805 | 1.4:-1668145 | MS.gene90712:CDS |
| GACTGGCAGGCAGGTTTCTT+AGG | 0.302431 | 1.4:-1667754 | MS.gene90712:CDS |
| AGACATGACCTTATTAATTA+GGG | 0.312033 | 1.4:+1667996 | None:intergenic |
| GGATGCTTTGTGCAAAATAT+AGG | 0.342315 | 1.4:-1667733 | MS.gene90712:CDS |
| CATTGGAACCCTAATTAATA+AGG | 0.369071 | 1.4:-1668004 | MS.gene90712:CDS |
| GTTTGCTGTTGAGAAGGTTC+TGG | 0.378464 | 1.4:-1667671 | MS.gene90712:CDS |
| TATGTATTGAACGCAATAAA+AGG | 0.379872 | 1.4:-1668050 | MS.gene90712:CDS |
| CGCAATAAAAGGTACTTTCA+TGG | 0.408821 | 1.4:-1668039 | MS.gene90712:CDS |
| AACAAAATCTGAGTGTTGTT+TGG | 0.428899 | 1.4:-1667648 | MS.gene90712:CDS |
| AACTCTTTCATGTTCCCTTA+CGG | 0.437754 | 1.4:-1667903 | MS.gene90712:CDS |
| ATTGTGGATGGATCCTCTTA+TGG | 0.440489 | 1.4:-1667814 | MS.gene90712:CDS |
| TTCTAGCATTGGTAGCAAAC+TGG | 0.448398 | 1.4:-1668118 | MS.gene90712:CDS |
| GTTAGAGAGTGATTCGTTGA+TGG | 0.456583 | 1.4:-1667626 | MS.gene90712:CDS |
| TGGTGTGCCTTGGCGAATGC+GGG | 0.459966 | 1.4:-1667572 | MS.gene90712:CDS |
| TTCTGGATTCATCCTGAGAT+GGG | 0.474859 | 1.4:-1667850 | MS.gene90712:CDS |
| ATCTTGATACATCCCATCTC+AGG | 0.483218 | 1.4:+1667838 | None:intergenic |
| AGCACCAATGGTGCTAATAG+AGG | 0.488742 | 1.4:+1667786 | None:intergenic |
| TCACCCTCTATTAGCACCAT+TGG | 0.489786 | 1.4:-1667790 | MS.gene90712:CDS |
| GTCTCTGAAAACAGCACCAA+TGG | 0.490668 | 1.4:+1667774 | None:intergenic |
| CAGTTGTTACAACAGTCCTC+GGG | 0.491364 | 1.4:+1667878 | None:intergenic |
| CTTTGGGATTGGCTTATGAA+AGG | 0.505933 | 1.4:-1668192 | None:intergenic |
| CTGGTGTGCCTTGGCGAATG+CGG | 0.511570 | 1.4:-1667573 | MS.gene90712:CDS |
| TTTCTGGATTCATCCTGAGA+TGG | 0.512416 | 1.4:-1667851 | MS.gene90712:CDS |
| TCAGTTGTTACAACAGTCCT+CGG | 0.518954 | 1.4:+1667877 | None:intergenic |
| CTGACATTGTTCATGTGCTA+TGG | 0.523669 | 1.4:-1668077 | MS.gene90712:CDS |
| TATAGGCCATGCTACACCAT+TGG | 0.527542 | 1.4:-1667716 | MS.gene90712:CDS |
| AATAGAGGGTGAGCCATAAG+AGG | 0.533805 | 1.4:+1667801 | None:intergenic |
| TTTAATACTAACGGTGATGC+TGG | 0.535648 | 1.4:-1667592 | MS.gene90712:CDS |
| CTTGGCGAATGCGGGTCTGA+TGG | 0.555327 | 1.4:-1667564 | None:intergenic |
| ACGGTGATGCTGGTGTGCCT+TGG | 0.556386 | 1.4:-1667582 | MS.gene90712:CDS |
| CATGGTATACAAGCTAACAT+TGG | 0.558801 | 1.4:-1668021 | MS.gene90712:CDS |
| TTTGATATAGTTCTTGCTAG+AGG | 0.559203 | 1.4:-1667958 | MS.gene90712:CDS |
| GTTGATGTTTGCTGTTGAGA+AGG | 0.579685 | 1.4:-1667677 | MS.gene90712:CDS |
| TGTATCAAGATAATTGTGGA+TGG | 0.586141 | 1.4:-1667826 | MS.gene90712:CDS |
| GCACCAATGGTGCTAATAGA+GGG | 0.587776 | 1.4:+1667787 | None:intergenic |
| CAGTTTCCAATGGTGTAGCA+TGG | 0.590868 | 1.4:+1667710 | None:intergenic |
| GCACTAAATTCAGTTTCCAA+TGG | 0.609361 | 1.4:+1667700 | None:intergenic |
| CATCAGACCCGCATTCGCCA+AGG | 0.613773 | 1.4:+1667565 | None:intergenic |
| GGGATGTATCAAGATAATTG+TGG | 0.635518 | 1.4:-1667830 | MS.gene90712:CDS |
| AATTAATAAGGTCATGTCTG+AGG | 0.638254 | 1.4:-1667992 | MS.gene90712:CDS |
| AGTTGTTACAACAGTCCTCG+GGG | 0.651989 | 1.4:+1667879 | None:intergenic |
| TATGCGAAAGTCTGTCATAG+AGG | 0.655581 | 1.4:+1667930 | None:intergenic |
| GTTGTTACAACAGTCCTCGG+GGG | 0.745786 | 1.4:+1667880 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTAATGCTTTTAATACTAA+CGG | - | chr1.4:1668143-1668162 | MS.gene90712:CDS | 20.0% |
| ! | AGACATGACCTTATTAATTA+GGG | + | chr1.4:1667751-1667770 | None:intergenic | 25.0% |
| ! | TATGTATTGAACGCAATAAA+AGG | - | chr1.4:1667694-1667713 | MS.gene90712:CDS | 25.0% |
| !! | TTGTAACAACTGATATTTTC+TGG | - | chr1.4:1667877-1667896 | MS.gene90712:CDS | 25.0% |
| AATTAATAAGGTCATGTCTG+AGG | - | chr1.4:1667752-1667771 | MS.gene90712:CDS | 30.0% | |
| CAGACATGACCTTATTAATT+AGG | + | chr1.4:1667752-1667771 | None:intergenic | 30.0% | |
| CATTGGAACCCTAATTAATA+AGG | - | chr1.4:1667740-1667759 | MS.gene90712:CDS | 30.0% | |
| TGTATCAAGATAATTGTGGA+TGG | - | chr1.4:1667918-1667937 | MS.gene90712:CDS | 30.0% | |
| TTTGATATAGTTCTTGCTAG+AGG | - | chr1.4:1667786-1667805 | MS.gene90712:CDS | 30.0% | |
| ! | AACAAAATCTGAGTGTTGTT+TGG | - | chr1.4:1668096-1668115 | MS.gene90712:CDS | 30.0% |
| ! | AATTGCTGCAATCTGATTTT+GGG | - | chr1.4:1667600-1667619 | MS.gene90712:CDS | 30.0% |
| AACTCTTTCATGTTCCCTTA+CGG | - | chr1.4:1667841-1667860 | MS.gene90712:CDS | 35.0% | |
| CATGGTATACAAGCTAACAT+TGG | - | chr1.4:1667723-1667742 | MS.gene90712:CDS | 35.0% | |
| GCACTAAATTCAGTTTCCAA+TGG | + | chr1.4:1668047-1668066 | None:intergenic | 35.0% | |
| GGATGCTTTGTGCAAAATAT+AGG | - | chr1.4:1668011-1668030 | MS.gene90712:CDS | 35.0% | |
| GGGATGTATCAAGATAATTG+TGG | - | chr1.4:1667914-1667933 | MS.gene90712:CDS | 35.0% | |
| TTTAATACTAACGGTGATGC+TGG | - | chr1.4:1668152-1668171 | MS.gene90712:CDS | 35.0% | |
| ! | GAATTGCTGCAATCTGATTT+TGG | - | chr1.4:1667599-1667618 | MS.gene90712:CDS | 35.0% |
| !! | ATTTTGGGTACTTCTAGCAT+TGG | - | chr1.4:1667615-1667634 | MS.gene90712:CDS | 35.0% |
| !! | CGCAATAAAAGGTACTTTCA+TGG | - | chr1.4:1667705-1667724 | MS.gene90712:CDS | 35.0% |
| ATCTTGATACATCCCATCTC+AGG | + | chr1.4:1667909-1667928 | None:intergenic | 40.0% | |
| ATTGTGGATGGATCCTCTTA+TGG | - | chr1.4:1667930-1667949 | MS.gene90712:CDS | 40.0% | |
| CTGACATTGTTCATGTGCTA+TGG | - | chr1.4:1667667-1667686 | MS.gene90712:CDS | 40.0% | |
| GTTAGAGAGTGATTCGTTGA+TGG | - | chr1.4:1668118-1668137 | MS.gene90712:CDS | 40.0% | |
| TATGCGAAAGTCTGTCATAG+AGG | + | chr1.4:1667817-1667836 | None:intergenic | 40.0% | |
| TCAGTTGTTACAACAGTCCT+CGG | + | chr1.4:1667870-1667889 | None:intergenic | 40.0% | |
| TTCTGGATTCATCCTGAGAT+GGG | - | chr1.4:1667894-1667913 | MS.gene90712:CDS | 40.0% | |
| TTTCTGGATTCATCCTGAGA+TGG | - | chr1.4:1667893-1667912 | MS.gene90712:CDS | 40.0% | |
| !! | GTTGATGTTTGCTGTTGAGA+AGG | - | chr1.4:1668067-1668086 | MS.gene90712:CDS | 40.0% |
| !! | TTCTAGCATTGGTAGCAAAC+TGG | - | chr1.4:1667626-1667645 | MS.gene90712:CDS | 40.0% |
| AATAGAGGGTGAGCCATAAG+AGG | + | chr1.4:1667946-1667965 | None:intergenic | 45.0% | |
| AGTTGTTACAACAGTCCTCG+GGG | + | chr1.4:1667868-1667887 | None:intergenic | 45.0% | |
| CAGTTGTTACAACAGTCCTC+GGG | + | chr1.4:1667869-1667888 | None:intergenic | 45.0% | |
| CAGTTTCCAATGGTGTAGCA+TGG | + | chr1.4:1668037-1668056 | None:intergenic | 45.0% | |
| TATAGGCCATGCTACACCAT+TGG | - | chr1.4:1668028-1668047 | MS.gene90712:CDS | 45.0% | |
| TCACCCTCTATTAGCACCAT+TGG | - | chr1.4:1667954-1667973 | MS.gene90712:CDS | 45.0% | |
| ! | AGCACCAATGGTGCTAATAG+AGG | + | chr1.4:1667961-1667980 | None:intergenic | 45.0% |
| ! | GCACCAATGGTGCTAATAGA+GGG | + | chr1.4:1667960-1667979 | None:intergenic | 45.0% |
| ! | GTCTCTGAAAACAGCACCAA+TGG | + | chr1.4:1667973-1667992 | None:intergenic | 45.0% |
| !! | GTTTGCTGTTGAGAAGGTTC+TGG | - | chr1.4:1668073-1668092 | MS.gene90712:CDS | 45.0% |
| !!! | TTGGTGCTGTTTTCAGAGAC+TGG | - | chr1.4:1667973-1667992 | MS.gene90712:CDS | 45.0% |
| GTTGTTACAACAGTCCTCGG+GGG | + | chr1.4:1667867-1667886 | None:intergenic | 50.0% | |
| !!! | TGCTGTTTTCAGAGACTGGC+AGG | - | chr1.4:1667977-1667996 | MS.gene90712:CDS | 50.0% |
| GACTGGCAGGCAGGTTTCTT+AGG | - | chr1.4:1667990-1668009 | MS.gene90712:CDS | 55.0% | |
| ! | GTTTTCAGAGACTGGCAGGC+AGG | - | chr1.4:1667981-1668000 | MS.gene90712:CDS | 55.0% |
| ACGGTGATGCTGGTGTGCCT+TGG | - | chr1.4:1668162-1668181 | MS.gene90712:CDS | 60.0% | |
| CTGGTGTGCCTTGGCGAATG+CGG | - | chr1.4:1668171-1668190 | MS.gene90712:CDS | 60.0% | |
| TGGTGTGCCTTGGCGAATGC+GGG | - | chr1.4:1668172-1668191 | MS.gene90712:CDS | 60.0% | |
| AACAGTCCTCGGGGGCCGTA+AGG | + | chr1.4:1667859-1667878 | None:intergenic | 65.0% | |
| ACAGTCCTCGGGGGCCGTAA+GGG | + | chr1.4:1667858-1667877 | None:intergenic | 65.0% | |
| ATGTTCCCTTACGGCCCCCG+AGG | - | chr1.4:1667850-1667869 | MS.gene90712:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 1667567 | 1668199 | 1667567 | ID=MS.gene90712 |
| chr1.4 | mRNA | 1667567 | 1668199 | 1667567 | ID=MS.gene90712.t1;Parent=MS.gene90712 |
| chr1.4 | exon | 1667567 | 1668199 | 1667567 | ID=MS.gene90712.t1.exon1;Parent=MS.gene90712.t1 |
| chr1.4 | CDS | 1667567 | 1668199 | 1667567 | ID=cds.MS.gene90712.t1;Parent=MS.gene90712.t1 |
| Gene Sequence |
| Protein sequence |
