Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056992.t1 | XP_003597557.1 | 99.5 | 188 | 1 | 0 | 1 | 188 | 1 | 188 | 4.30E-94 | 354 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056992.t1 | Q38820 | 64.9 | 185 | 62 | 1 | 7 | 188 | 4 | 188 | 3.5e-60 | 232.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056992.t1 | G7IS28 | 99.5 | 188 | 1 | 0 | 1 | 188 | 1 | 188 | 3.1e-94 | 354.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050838 | MS.gene056992 | 0.821098 | 4.84E-53 | -1.69E-46 |
| MS.gene051809 | MS.gene056992 | 0.809226 | 2.10E-50 | -1.69E-46 |
| MS.gene051969 | MS.gene056992 | 0.880901 | 3.75E-70 | -1.69E-46 |
| MS.gene056992 | MS.gene057094 | 0.820799 | 5.67E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene057721 | 0.815235 | 1.03E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene057999 | 0.812904 | 3.35E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene058758 | 0.803998 | 2.67E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene06131 | 0.816071 | 6.68E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene061898 | 0.822796 | 1.96E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene062117 | 0.816938 | 4.27E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene067043 | 0.819925 | 8.99E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene069225 | 0.815657 | 8.26E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene06998 | 0.812498 | 4.11E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene070671 | 0.803156 | 3.99E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene072057 | 0.81934 | 1.22E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene072328 | 0.826862 | 2.15E-54 | -1.69E-46 |
| MS.gene056992 | MS.gene072329 | 0.82454 | 7.64E-54 | -1.69E-46 |
| MS.gene056992 | MS.gene072641 | 0.809687 | 1.67E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene072969 | 0.8111 | 8.29E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene07339 | 0.800688 | 1.28E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene07506 | 0.800299 | 1.54E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene08793 | 0.801056 | 1.08E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene08922 | 0.805428 | 1.34E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene09032 | 0.803078 | 4.14E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene09852 | 0.816393 | 5.66E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene23484 | 0.80055 | 1.37E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene23935 | 0.834742 | 2.51E-56 | -1.69E-46 |
| MS.gene056992 | MS.gene24838 | 0.821088 | 4.86E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene26426 | 0.813668 | 2.28E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene26605 | 0.812528 | 4.05E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene26685 | -0.801848 | 7.42E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene26985 | 0.84705 | 1.48E-59 | -1.69E-46 |
| MS.gene056992 | MS.gene27678 | 0.809171 | 2.16E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene28159 | 0.804183 | 2.44E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene28409 | 0.805152 | 1.53E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene28632 | 0.81105 | 8.50E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene29047 | 0.852781 | 3.69E-61 | -1.69E-46 |
| MS.gene056992 | MS.gene29109 | 0.830723 | 2.50E-55 | -1.69E-46 |
| MS.gene056992 | MS.gene29719 | 0.832461 | 9.33E-56 | -1.69E-46 |
| MS.gene056992 | MS.gene29794 | 0.805735 | 1.16E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene32193 | 0.814523 | 1.48E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene36627 | 0.810071 | 1.38E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene38070 | 0.813122 | 3.00E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene38145 | 0.838041 | 3.64E-57 | -1.69E-46 |
| MS.gene056992 | MS.gene38262 | 0.834206 | 3.43E-56 | -1.69E-46 |
| MS.gene056992 | MS.gene39124 | 0.803076 | 4.14E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene40055 | 0.819542 | 1.10E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene40220 | 0.83306 | 6.62E-56 | -1.69E-46 |
| MS.gene056992 | MS.gene42295 | 0.813466 | 2.52E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene45785 | 0.811953 | 5.41E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene46392 | 0.800582 | 1.35E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene47674 | 0.81102 | 8.63E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene47985 | 0.820472 | 6.74E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene47995 | 0.85037 | 1.77E-60 | -1.69E-46 |
| MS.gene056992 | MS.gene48283 | 0.820973 | 5.17E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene49733 | 0.836926 | 7.03E-57 | -1.69E-46 |
| MS.gene056992 | MS.gene49777 | 0.817456 | 3.27E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene51087 | 0.808249 | 3.40E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene51445 | 0.811296 | 7.52E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene51557 | 0.818058 | 2.39E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene51920 | 0.840381 | 9.00E-58 | -1.69E-46 |
| MS.gene056992 | MS.gene52281 | 0.826937 | 2.06E-54 | -1.69E-46 |
| MS.gene056992 | MS.gene54163 | 0.803906 | 2.79E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene55282 | 0.829803 | 4.19E-55 | -1.69E-46 |
| MS.gene056992 | MS.gene57348 | 0.819221 | 1.30E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene57847 | 0.817627 | 2.99E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene58098 | 0.808205 | 3.47E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene58438 | 0.810423 | 1.16E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene58616 | 0.811934 | 5.46E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene58768 | 0.812506 | 4.10E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene58769 | 0.810805 | 9.60E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene58793 | 0.813023 | 3.16E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene60265 | 0.810721 | 1.00E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene60372 | 0.824585 | 7.45E-54 | -1.69E-46 |
| MS.gene056992 | MS.gene65554 | 0.847048 | 1.48E-59 | -1.69E-46 |
| MS.gene056992 | MS.gene66089 | 0.800168 | 1.64E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene66119 | 0.820861 | 5.48E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene66818 | 0.83489 | 2.31E-56 | -1.69E-46 |
| MS.gene056992 | MS.gene67269 | 0.810728 | 9.98E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene67881 | 0.805421 | 1.35E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene69097 | 0.816263 | 6.05E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene69691 | 0.806387 | 8.43E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene69692 | 0.806649 | 7.42E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene70530 | 0.802617 | 5.15E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene70596 | 0.801164 | 1.02E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene71994 | 0.822903 | 1.85E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene72933 | 0.804091 | 2.55E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene74508 | 0.805579 | 1.25E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene74672 | 0.816954 | 4.24E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene74919 | 0.807222 | 5.62E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene75412 | 0.821806 | 3.32E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene76478 | 0.814788 | 1.29E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene80269 | 0.82315 | 1.62E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene81288 | 0.822181 | 2.72E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene83022 | 0.830213 | 3.33E-55 | -1.69E-46 |
| MS.gene056992 | MS.gene85610 | 0.810239 | 1.27E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene86937 | 0.800498 | 1.40E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene88427 | 0.823837 | 1.12E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene88859 | 0.814066 | 1.86E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene89663 | 0.811547 | 6.63E-51 | -1.69E-46 |
| MS.gene056992 | MS.gene90698 | 0.848423 | 6.19E-60 | -1.69E-46 |
| MS.gene056992 | MS.gene90712 | -0.828934 | 6.82E-55 | -1.69E-46 |
| MS.gene056992 | MS.gene90965 | 0.81735 | 3.45E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene91524 | 0.816464 | 5.46E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene92135 | 0.800376 | 1.48E-48 | -1.69E-46 |
| MS.gene056992 | MS.gene93983 | 0.802472 | 5.52E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene94183 | 0.818998 | 1.46E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene94992 | 0.827428 | 1.57E-54 | -1.69E-46 |
| MS.gene056992 | MS.gene95332 | 0.802215 | 6.24E-49 | -1.69E-46 |
| MS.gene056992 | MS.gene96245 | 0.808597 | 2.86E-50 | -1.69E-46 |
| MS.gene056992 | MS.gene96690 | 0.813522 | 2.45E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056992.t1 | MTR_2g099550 | 99.468 | 188 | 1 | 0 | 1 | 188 | 1 | 188 | 1.39e-137 | 382 |
| MS.gene056992.t1 | MTR_8g018780 | 75.419 | 179 | 44 | 0 | 10 | 188 | 4 | 182 | 2.49e-85 | 249 |
| MS.gene056992.t1 | MTR_1g081230 | 61.749 | 183 | 69 | 1 | 7 | 188 | 3 | 185 | 6.72e-76 | 226 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056992.t1 | AT1G72750 | 67.568 | 185 | 57 | 1 | 7 | 188 | 4 | 188 | 2.31e-76 | 227 |
| MS.gene056992.t1 | AT1G17530 | 62.366 | 186 | 64 | 2 | 5 | 188 | 6 | 187 | 1.61e-74 | 222 |
| MS.gene056992.t1 | AT1G17530 | 62.295 | 183 | 63 | 2 | 4 | 184 | 5 | 183 | 3.36e-73 | 219 |
| MS.gene056992.t1 | AT3G04800 | 52.459 | 183 | 86 | 1 | 7 | 188 | 6 | 188 | 7.56e-51 | 162 |
Find 76 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCAGCAGGGCTTGGTACC+GGG | 0.325322 | 2.1:-5056299 | MS.gene056992:CDS |
| TTGGGAGCTGGTAGAGGTTT+CGG | 0.328905 | 2.1:+5056631 | None:intergenic |
| TAGGGATTGTAGAGACGTTT+TGG | 0.331643 | 2.1:+5056675 | None:intergenic |
| GTAGCCGCATCCGGTGTAAA+AGG | 0.338122 | 2.1:+5056542 | None:intergenic |
| TTGGTACCGGGGCGTTGTAT+CGG | 0.348837 | 2.1:-5056287 | MS.gene056992:CDS |
| ACCTTTCTCGAACGATTTCA+CGG | 0.355472 | 2.1:+5056476 | None:intergenic |
| GTGGCAGCAGGGCTTGGTAC+CGG | 0.372617 | 2.1:-5056300 | MS.gene056992:CDS |
| AAGAGATGTTCAGGTGAGGT+TGG | 0.384154 | 2.1:+5056612 | None:intergenic |
| TCCTCGATGAAGAGATGTTC+AGG | 0.386072 | 2.1:+5056603 | None:intergenic |
| CAGGACACACGGGTCGGACG+TGG | 0.387337 | 2.1:-5056413 | MS.gene056992:CDS |
| GGCTACCTCGGCGGCGCCAT+TGG | 0.387745 | 2.1:-5056525 | MS.gene056992:CDS |
| TGGTACCGGGGCGTTGTATC+GGG | 0.396607 | 2.1:-5056286 | MS.gene056992:CDS |
| GTGAGATCAGCTGCTGTTGC+CGG | 0.398096 | 2.1:-5056252 | MS.gene056992:CDS |
| CGCATCCGGTGTAAAAGGTT+AGG | 0.399415 | 2.1:+5056547 | None:intergenic |
| GCCGCCGAGGTAGCCGCATC+CGG | 0.401744 | 2.1:+5056533 | None:intergenic |
| GGGAACCGCCTTGGTGTGAT+CGG | 0.402790 | 2.1:-5056390 | MS.gene056992:CDS |
| TCGGAGTTTGATTCGTGATC+GGG | 0.411466 | 2.1:+5056708 | None:intergenic |
| CACAACACCACCAACAGCAC+CGG | 0.411667 | 2.1:+5056233 | None:intergenic |
| GGCGGCGCCATTGGTGGCGC+CGG | 0.412036 | 2.1:-5056516 | MS.gene056992:CDS |
| AACAGTGTGGCAGCAGGGCT+TGG | 0.418069 | 2.1:-5056306 | MS.gene056992:CDS |
| GCTGTTGGTGGTGTTGTGGC+TGG | 0.423812 | 2.1:-5056228 | MS.gene056992:CDS |
| GGAACCGCCTTGGTGTGATC+GGG | 0.429194 | 2.1:-5056389 | MS.gene056992:CDS |
| TACCTCGGCGGCGCCATTGG+TGG | 0.432819 | 2.1:-5056522 | MS.gene056992:CDS |
| CGTTGTATCGGGCAGCGAGA+GGG | 0.437956 | 2.1:-5056275 | MS.gene056992:CDS |
| GTGAGGTTGGGAGCTGGTAG+AGG | 0.443193 | 2.1:+5056625 | None:intergenic |
| GGATTGGTACTTCGAGGTCT+TGG | 0.447073 | 2.1:+5056652 | None:intergenic |
| CTCAACGCAAACATCGTTCC+TGG | 0.447403 | 2.1:-5056578 | MS.gene056992:CDS |
| GTGGCTGGAGTGGCTATCGC+GGG | 0.448335 | 2.1:-5056213 | MS.gene056992:CDS |
| AGTGGCTATCGCGGGGAAGC+AGG | 0.450298 | 2.1:-5056205 | MS.gene056992:CDS |
| AAAGGTTAGGGTTTCTCCCC+AGG | 0.463183 | 2.1:+5056560 | None:intergenic |
| GCCATTGGTGGCGCCGGCGT+AGG | 0.463542 | 2.1:-5056510 | MS.gene056992:CDS |
| GCTGCTGTTGCCGGTGCTGT+TGG | 0.465020 | 2.1:-5056243 | MS.gene056992:CDS |
| GGACACACGGGTCGGACGTG+GGG | 0.487258 | 2.1:-5056411 | MS.gene056992:CDS |
| GCATCCGGTGTAAAAGGTTA+GGG | 0.487745 | 2.1:+5056548 | None:intergenic |
| AACAAGATTCTGAATTCGTC+AGG | 0.489468 | 2.1:-5056432 | MS.gene056992:CDS |
| AGAGATGTTCAGGTGAGGTT+GGG | 0.491614 | 2.1:+5056613 | None:intergenic |
| ATCGGAGTTTGATTCGTGAT+CGG | 0.492854 | 2.1:+5056707 | None:intergenic |
| TGGTACTTCGAGGTCTTGGT+AGG | 0.496614 | 2.1:+5056656 | None:intergenic |
| GCCGTGAAATCGTTCGAGAA+AGG | 0.498953 | 2.1:-5056477 | MS.gene056992:CDS |
| CGCCACCAATGGCGCCGCCG+AGG | 0.504816 | 2.1:+5056520 | None:intergenic |
| GCTGTTGCCGGTGCTGTTGG+TGG | 0.505954 | 2.1:-5056240 | MS.gene056992:CDS |
| GGTTTCGGATTGGTACTTCG+AGG | 0.508805 | 2.1:+5056646 | None:intergenic |
| GTTGTATCGGGCAGCGAGAG+GGG | 0.520290 | 2.1:-5056274 | MS.gene056992:CDS |
| ACCTACGCCGGCGCCACCAA+TGG | 0.521262 | 2.1:+5056509 | None:intergenic |
| GCGTTGTATCGGGCAGCGAG+AGG | 0.529365 | 2.1:-5056276 | MS.gene056992:CDS |
| TCAACGCAAACATCGTTCCT+GGG | 0.532503 | 2.1:-5056577 | MS.gene056992:CDS |
| AGGACACACGGGTCGGACGT+GGG | 0.534760 | 2.1:-5056412 | MS.gene056992:CDS |
| GGCAGCAGGGCTTGGTACCG+GGG | 0.541292 | 2.1:-5056298 | MS.gene056992:CDS |
| TGGAATTGAAAGCGGGATCA+CGG | 0.541386 | 2.1:-5056355 | MS.gene056992:CDS |
| CGGTGCTGTTGGTGGTGTTG+TGG | 0.552191 | 2.1:-5056232 | MS.gene056992:CDS |
| GATCACGGCGGCGAGAGATA+CGG | 0.553451 | 2.1:-5056340 | MS.gene056992:CDS |
| TACACCGGATGCGGCTACCT+CGG | 0.560580 | 2.1:-5056537 | MS.gene056992:CDS |
| GTGATCGGGTTGTTATTCGC+TGG | 0.562580 | 2.1:-5056375 | MS.gene056992:CDS |
| GTTCAGGTGAGGTTGGGAGC+TGG | 0.563872 | 2.1:+5056619 | None:intergenic |
| TGTGGCTGGAGTGGCTATCG+CGG | 0.570123 | 2.1:-5056214 | MS.gene056992:CDS |
| TCTGAATTCGTCAGGACACA+CGG | 0.572872 | 2.1:-5056424 | MS.gene056992:CDS |
| TGGTGGTGTTGTGGCTGGAG+TGG | 0.577673 | 2.1:-5056223 | MS.gene056992:CDS |
| GACACACGGGTCGGACGTGG+GGG | 0.579942 | 2.1:-5056410 | MS.gene056992:CDS |
| CGGAGTTTGATTCGTGATCG+GGG | 0.580921 | 2.1:+5056709 | None:intergenic |
| GGTACTTCGAGGTCTTGGTA+GGG | 0.582086 | 2.1:+5056657 | None:intergenic |
| TATTCGCTGGAATTGAAAGC+GGG | 0.588545 | 2.1:-5056362 | MS.gene056992:CDS |
| AGCTGGTAGAGGTTTCGGAT+TGG | 0.594257 | 2.1:+5056636 | None:intergenic |
| ACCGGATGCGGCTACCTCGG+CGG | 0.600899 | 2.1:-5056534 | MS.gene056992:CDS |
| CTGAATTCGTCAGGACACAC+GGG | 0.613772 | 2.1:-5056423 | MS.gene056992:CDS |
| CGCTGCCCGATACAACGCCC+CGG | 0.635857 | 2.1:+5056281 | None:intergenic |
| TTATTCGCTGGAATTGAAAG+CGG | 0.641254 | 2.1:-5056363 | MS.gene056992:CDS |
| GGAGTTTGATTCGTGATCGG+GGG | 0.642744 | 2.1:+5056710 | None:intergenic |
| TGGCTGGAGTGGCTATCGCG+GGG | 0.652115 | 2.1:-5056212 | MS.gene056992:CDS |
| ATTCGTCAGGACACACGGGT+CGG | 0.661517 | 2.1:-5056419 | MS.gene056992:CDS |
| CAACGCAAACATCGTTCCTG+GGG | 0.671475 | 2.1:-5056576 | MS.gene056992:CDS |
| GGCTTCGACGAAACCTACGC+CGG | 0.685279 | 2.1:+5056497 | None:intergenic |
| GATGAAGAGATGTTCAGGTG+AGG | 0.693036 | 2.1:+5056608 | None:intergenic |
| ACCTGAACATCTCTTCATCG+AGG | 0.710068 | 2.1:-5056604 | MS.gene056992:CDS |
| ATAACAACCCGATCACACCA+AGG | 0.726478 | 2.1:+5056382 | None:intergenic |
| AATTGAAAGCGGGATCACGG+CGG | 0.736385 | 2.1:-5056352 | MS.gene056992:CDS |
| ACAACCCGATCACACCAAGG+CGG | 0.775839 | 2.1:+5056385 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAGATTCTGAATTCGTC+AGG | - | chr2.1:5056470-5056489 | MS.gene056992:CDS | 35.0% | |
| TTATTCGCTGGAATTGAAAG+CGG | - | chr2.1:5056539-5056558 | MS.gene056992:CDS | 35.0% | |
| ACCTTTCTCGAACGATTTCA+CGG | + | chr2.1:5056429-5056448 | None:intergenic | 40.0% | |
| TATTCGCTGGAATTGAAAGC+GGG | - | chr2.1:5056540-5056559 | MS.gene056992:CDS | 40.0% | |
| ! | ATCGGAGTTTGATTCGTGAT+CGG | + | chr2.1:5056198-5056217 | None:intergenic | 40.0% |
| ! | GAAACCCTAACCTTTTACAC+CGG | - | chr2.1:5056350-5056369 | MS.gene056992:CDS | 40.0% |
| ! | TAGGGATTGTAGAGACGTTT+TGG | + | chr2.1:5056230-5056249 | None:intergenic | 40.0% |
| !! | GATTGTAGAGACGTTTTGGA+GGG | + | chr2.1:5056226-5056245 | None:intergenic | 40.0% |
| AAGAGATGTTCAGGTGAGGT+TGG | + | chr2.1:5056293-5056312 | None:intergenic | 45.0% | |
| ACCTGAACATCTCTTCATCG+AGG | - | chr2.1:5056298-5056317 | MS.gene056992:CDS | 45.0% | |
| AGAGATGTTCAGGTGAGGTT+GGG | + | chr2.1:5056292-5056311 | None:intergenic | 45.0% | |
| ATAACAACCCGATCACACCA+AGG | + | chr2.1:5056523-5056542 | None:intergenic | 45.0% | |
| GATGAAGAGATGTTCAGGTG+AGG | + | chr2.1:5056297-5056316 | None:intergenic | 45.0% | |
| GCATCCGGTGTAAAAGGTTA+GGG | + | chr2.1:5056357-5056376 | None:intergenic | 45.0% | |
| TCAACGCAAACATCGTTCCT+GGG | - | chr2.1:5056325-5056344 | MS.gene056992:CDS | 45.0% | |
| TCCTCGATGAAGAGATGTTC+AGG | + | chr2.1:5056302-5056321 | None:intergenic | 45.0% | |
| TCTGAATTCGTCAGGACACA+CGG | - | chr2.1:5056478-5056497 | MS.gene056992:CDS | 45.0% | |
| TGGAATTGAAAGCGGGATCA+CGG | - | chr2.1:5056547-5056566 | MS.gene056992:CDS | 45.0% | |
| ! | CTAACCTTTTACACCGGATG+CGG | - | chr2.1:5056356-5056375 | MS.gene056992:CDS | 45.0% |
| ! | GGACGATGTTTTCAACAGTG+TGG | - | chr2.1:5056583-5056602 | MS.gene056992:CDS | 45.0% |
| ! | GGATTGTAGAGACGTTTTGG+AGG | + | chr2.1:5056227-5056246 | None:intergenic | 45.0% |
| ! | TCGGAGTTTGATTCGTGATC+GGG | + | chr2.1:5056197-5056216 | None:intergenic | 45.0% |
| ! | TTTTCAACAGTGTGGCAGCA+GGG | - | chr2.1:5056591-5056610 | MS.gene056992:CDS | 45.0% |
| !!! | ACGTTTTGGAGGGTTGGAAT+CGG | + | chr2.1:5056216-5056235 | None:intergenic | 45.0% |
| AAAGGTTAGGGTTTCTCCCC+AGG | + | chr2.1:5056345-5056364 | None:intergenic | 50.0% | |
| AATTGAAAGCGGGATCACGG+CGG | - | chr2.1:5056550-5056569 | MS.gene056992:CDS | 50.0% | |
| AGCTGGTAGAGGTTTCGGAT+TGG | + | chr2.1:5056269-5056288 | None:intergenic | 50.0% | |
| CAACGCAAACATCGTTCCTG+GGG | - | chr2.1:5056326-5056345 | MS.gene056992:CDS | 50.0% | |
| CGCATCCGGTGTAAAAGGTT+AGG | + | chr2.1:5056358-5056377 | None:intergenic | 50.0% | |
| CTCAACGCAAACATCGTTCC+TGG | - | chr2.1:5056324-5056343 | MS.gene056992:CDS | 50.0% | |
| CTGAATTCGTCAGGACACAC+GGG | - | chr2.1:5056479-5056498 | MS.gene056992:CDS | 50.0% | |
| GCCGTGAAATCGTTCGAGAA+AGG | - | chr2.1:5056425-5056444 | MS.gene056992:CDS | 50.0% | |
| GGTACTTCGAGGTCTTGGTA+GGG | + | chr2.1:5056248-5056267 | None:intergenic | 50.0% | |
| TGGTACTTCGAGGTCTTGGT+AGG | + | chr2.1:5056249-5056268 | None:intergenic | 50.0% | |
| TTGGGAGCTGGTAGAGGTTT+CGG | + | chr2.1:5056274-5056293 | None:intergenic | 50.0% | |
| ! | CGGAGTTTGATTCGTGATCG+GGG | + | chr2.1:5056196-5056215 | None:intergenic | 50.0% |
| ! | GGAGTTTGATTCGTGATCGG+GGG | + | chr2.1:5056195-5056214 | None:intergenic | 50.0% |
| ! | GGATTGGTACTTCGAGGTCT+TGG | + | chr2.1:5056253-5056272 | None:intergenic | 50.0% |
| ! | GTGATCGGGTTGTTATTCGC+TGG | - | chr2.1:5056527-5056546 | MS.gene056992:CDS | 50.0% |
| ! | GTTTTCAACAGTGTGGCAGC+AGG | - | chr2.1:5056590-5056609 | MS.gene056992:CDS | 50.0% |
| !! | GGTTTCGGATTGGTACTTCG+AGG | + | chr2.1:5056259-5056278 | None:intergenic | 50.0% |
| !!! | GTAGAGACGTTTTGGAGGGT+TGG | + | chr2.1:5056222-5056241 | None:intergenic | 50.0% |
| ACAACCCGATCACACCAAGG+CGG | + | chr2.1:5056520-5056539 | None:intergenic | 55.0% | |
| ATTCGTCAGGACACACGGGT+CGG | - | chr2.1:5056483-5056502 | MS.gene056992:CDS | 55.0% | |
| CACAACACCACCAACAGCAC+CGG | + | chr2.1:5056672-5056691 | None:intergenic | 55.0% | |
| GTAGCCGCATCCGGTGTAAA+AGG | + | chr2.1:5056363-5056382 | None:intergenic | 55.0% | |
| GTGAGATCAGCTGCTGTTGC+CGG | - | chr2.1:5056650-5056669 | MS.gene056992:CDS | 55.0% | |
| !! | TTGGTACCGGGGCGTTGTAT+CGG | - | chr2.1:5056615-5056634 | MS.gene056992:CDS | 55.0% |
| AACAGTGTGGCAGCAGGGCT+TGG | - | chr2.1:5056596-5056615 | MS.gene056992:CDS | 60.0% | |
| CGTTGTATCGGGCAGCGAGA+GGG | - | chr2.1:5056627-5056646 | MS.gene056992:CDS | 60.0% | |
| GATCACGGCGGCGAGAGATA+CGG | - | chr2.1:5056562-5056581 | MS.gene056992:CDS | 60.0% | |
| GGCTTCGACGAAACCTACGC+CGG | + | chr2.1:5056408-5056427 | None:intergenic | 60.0% | |
| GTGAGGTTGGGAGCTGGTAG+AGG | + | chr2.1:5056280-5056299 | None:intergenic | 60.0% | |
| GTTCAGGTGAGGTTGGGAGC+TGG | + | chr2.1:5056286-5056305 | None:intergenic | 60.0% | |
| GTTGTATCGGGCAGCGAGAG+GGG | - | chr2.1:5056628-5056647 | MS.gene056992:CDS | 60.0% | |
| TACACCGGATGCGGCTACCT+CGG | - | chr2.1:5056365-5056384 | MS.gene056992:CDS | 60.0% | |
| TGTGGCTGGAGTGGCTATCG+CGG | - | chr2.1:5056688-5056707 | MS.gene056992:CDS | 60.0% | |
| ! | GGAACCGCCTTGGTGTGATC+GGG | - | chr2.1:5056513-5056532 | MS.gene056992:CDS | 60.0% |
| ! | GGGAACCGCCTTGGTGTGAT+CGG | - | chr2.1:5056512-5056531 | MS.gene056992:CDS | 60.0% |
| !! | CGGTGCTGTTGGTGGTGTTG+TGG | - | chr2.1:5056670-5056689 | MS.gene056992:CDS | 60.0% |
| !! | GCTGTTGGTGGTGTTGTGGC+TGG | - | chr2.1:5056674-5056693 | MS.gene056992:CDS | 60.0% |
| !! | TGGTACCGGGGCGTTGTATC+GGG | - | chr2.1:5056616-5056635 | MS.gene056992:CDS | 60.0% |
| !! | TGGTGGTGTTGTGGCTGGAG+TGG | - | chr2.1:5056679-5056698 | MS.gene056992:CDS | 60.0% |
| AGGACACACGGGTCGGACGT+GGG | - | chr2.1:5056490-5056509 | MS.gene056992:CDS | 65.0% | |
| AGTGGCTATCGCGGGGAAGC+AGG | - | chr2.1:5056697-5056716 | MS.gene056992:CDS | 65.0% | |
| GCGTTGTATCGGGCAGCGAG+AGG | - | chr2.1:5056626-5056645 | MS.gene056992:CDS | 65.0% | |
| GTGGCTGGAGTGGCTATCGC+GGG | - | chr2.1:5056689-5056708 | MS.gene056992:CDS | 65.0% | |
| TGGCTGGAGTGGCTATCGCG+GGG | - | chr2.1:5056690-5056709 | MS.gene056992:CDS | 65.0% | |
| ! | GTGGCAGCAGGGCTTGGTAC+CGG | - | chr2.1:5056602-5056621 | MS.gene056992:CDS | 65.0% |
| ! | TGGCAGCAGGGCTTGGTACC+GGG | - | chr2.1:5056603-5056622 | MS.gene056992:CDS | 65.0% |
| !! | GCTGCTGTTGCCGGTGCTGT+TGG | - | chr2.1:5056659-5056678 | MS.gene056992:CDS | 65.0% |
| !! | GCTGTTGCCGGTGCTGTTGG+TGG | - | chr2.1:5056662-5056681 | MS.gene056992:CDS | 65.0% |
| ACCGGATGCGGCTACCTCGG+CGG | - | chr2.1:5056368-5056387 | MS.gene056992:CDS | 70.0% | |
| ACCTACGCCGGCGCCACCAA+TGG | + | chr2.1:5056396-5056415 | None:intergenic | 70.0% | |
| CAGGACACACGGGTCGGACG+TGG | - | chr2.1:5056489-5056508 | MS.gene056992:CDS | 70.0% | |
| CGCTGCCCGATACAACGCCC+CGG | + | chr2.1:5056624-5056643 | None:intergenic | 70.0% | |
| GACACACGGGTCGGACGTGG+GGG | - | chr2.1:5056492-5056511 | MS.gene056992:CDS | 70.0% | |
| GGACACACGGGTCGGACGTG+GGG | - | chr2.1:5056491-5056510 | MS.gene056992:CDS | 70.0% | |
| TACCTCGGCGGCGCCATTGG+TGG | - | chr2.1:5056380-5056399 | MS.gene056992:CDS | 70.0% | |
| ! | GGCAGCAGGGCTTGGTACCG+GGG | - | chr2.1:5056604-5056623 | MS.gene056992:CDS | 70.0% |
| CGGACGTGGGGGAACCGCCT+TGG | - | chr2.1:5056503-5056522 | MS.gene056992:CDS | 75.0% | |
| GCCGCCGAGGTAGCCGCATC+CGG | + | chr2.1:5056372-5056391 | None:intergenic | 75.0% | |
| GGCTACCTCGGCGGCGCCAT+TGG | - | chr2.1:5056377-5056396 | MS.gene056992:CDS | 75.0% | |
| !! | GCCATTGGTGGCGCCGGCGT+AGG | - | chr2.1:5056392-5056411 | MS.gene056992:CDS | 75.0% |
| ! | CGCCACCAATGGCGCCGCCG+AGG | + | chr2.1:5056385-5056404 | None:intergenic | 80.0% |
| !!! | GGCGGCGCCATTGGTGGCGC+CGG | - | chr2.1:5056386-5056405 | MS.gene056992:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 5056179 | 5056745 | 5056179 | ID=MS.gene056992 |
| chr2.1 | mRNA | 5056179 | 5056745 | 5056179 | ID=MS.gene056992.t1;Parent=MS.gene056992 |
| chr2.1 | exon | 5056179 | 5056745 | 5056179 | ID=MS.gene056992.t1.exon1;Parent=MS.gene056992.t1 |
| chr2.1 | CDS | 5056179 | 5056745 | 5056179 | ID=cds.MS.gene056992.t1;Parent=MS.gene056992.t1 |
| Gene Sequence |
| Protein sequence |
