AlfalfaGEDB | MS.gene90754

M. sativa cultivar XinJiangDaYe / MS.gene90754

KEGG: K02872
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90754.t1	XP_003589380.1	100	115	0	0	1	115	92	206	1.70E-56	228.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90754.t1	O49885	80.0	115	23	0	1	115	91	205	2.3e-46	186.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90754.t1	B7FMG6	100.0	115	0	0	1	115	92	206	1.2e-56	228.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052664	MS.gene90754	0.800127	1.67E-48	-1.69E-46
MS.gene056917	MS.gene90754	0.82607	3.32E-54	-1.69E-46
MS.gene057092	MS.gene90754	0.811081	8.37E-51	-1.69E-46
MS.gene057235	MS.gene90754	0.823414	1.40E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90754.t1	MTR_1g023590	100.000	115	0	1	115	92	206	6.96e-78	228
MS.gene90754.t1	MTR_7g057770	90.435	115	11	1	115	92	206	1.71e-70	209
MS.gene90754.t1	MTR_5g083790	87.826	115	14	1	115	92	206	7.41e-68	203

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90754.t1	AT4G13170	79.130	115	24	0	1	115	92	206	5.00e-62	188
MS.gene90754.t1	AT5G48760	79.130	115	24	0	1	115	92	206	2.26e-61	186
MS.gene90754.t1	AT5G48760	79.130	115	24	0	1	115	92	206	2.26e-61	186
MS.gene90754.t1	AT3G07110	79.130	115	24	0	1	115	92	206	2.06e-60	184
MS.gene90754.t1	AT3G24830	76.522	115	27	0	1	115	92	206	3.30e-59	181
MS.gene90754.t1	AT3G07110	78.448	116	24	1	1	115	92	207	1.09e-58	179

Find 36 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGAGCAAGGACTTCAAGTT+GGG	0.380650	1.3:+1652811	None:intergenic
TATAGGAGCTGGAGGATAAG+AGG	0.397991	1.3:-1652925	MS.gene90754:intron
GGCTGCACTCGCGCGATTGA+AGG	0.416813	1.3:-1653286	MS.gene90754:CDS
GAGGAAGGAACGCGCAGGTT+TGG	0.417217	1.3:-1652906	MS.gene90754:CDS
TAGGGTGTTGAGACTTCAAA+AGG	0.434162	1.3:-1653103	MS.gene90754:CDS
ATCCTCTTGATCTTGTCATA+TGG	0.437541	1.3:+1653243	None:intergenic
CGGGAGCAAGGACTTCAAGT+TGG	0.439216	1.3:+1652810	None:intergenic
AATAGTACTCAGTACTTAAC+GGG	0.449959	1.3:+1652791	None:intergenic
AAATAGTACTCAGTACTTAA+CGG	0.452401	1.3:+1652790	None:intergenic
GGTTCTTACTTGAGAGCATC+GGG	0.471713	1.3:+1653213	None:intergenic
AGAAGCTGCTCAATAAATTG+AGG	0.480348	1.3:-1652871	MS.gene90754:CDS
CGCGCGATTGAAGGTCTATG+AGG	0.509761	1.3:-1653277	MS.gene90754:CDS
GAAGCTGCTCAATAAATTGA+GGG	0.519049	1.3:-1652870	MS.gene90754:CDS
TGGTTCTTACTTGAGAGCAT+CGG	0.539745	1.3:+1653212	None:intergenic
TTCTTGTTTATAGGAGCTGG+AGG	0.546787	1.3:-1652933	MS.gene90754:intron
GGACACAAATACTGCTTGCT+CGG	0.550212	1.3:-1653081	MS.gene90754:CDS
AGTTGTCATCAGAGGTTGGA+TGG	0.565112	1.3:-1653056	MS.gene90754:CDS
TTGATCTTGTCATATGGAGG+AGG	0.574582	1.3:+1653249	None:intergenic
CTCCATATGACAAGATCAAG+AGG	0.577162	1.3:-1653245	MS.gene90754:CDS
TCAGAGGTTGGATGGAACTA+CGG	0.586349	1.3:-1653048	MS.gene90754:CDS
GGAGCTGGAGGATAAGAGGA+AGG	0.587458	1.3:-1652921	MS.gene90754:CDS
GAACTACGGTGGCATCATCA+AGG	0.590785	1.3:-1653034	MS.gene90754:intron
GGACAGTTGTCATCAGAGGT+TGG	0.592395	1.3:-1653060	MS.gene90754:CDS
CTCTTGATCTTGTCATATGG+AGG	0.593959	1.3:+1653246	None:intergenic
GCTCGGACAGTTGTCATCAG+AGG	0.597789	1.3:-1653064	MS.gene90754:CDS
AGGGTGTTGAGACTTCAAAA+GGG	0.620018	1.3:-1653102	MS.gene90754:CDS
GCGCGATTGAAGGTCTATGA+GGG	0.624683	1.3:-1653276	MS.gene90754:CDS
ATATGACAAGATCAAGAGGA+TGG	0.634497	1.3:-1653241	MS.gene90754:CDS
GGAGCAAGGACTTCAAGTTG+GGG	0.640419	1.3:+1652812	None:intergenic
TCTCCACGCTTAGTCTTGTG+TGG	0.642845	1.3:+1653309	None:intergenic
ATACCACACAAGACTAAGCG+TGG	0.643021	1.3:-1653312	MS.gene90754:CDS
AGCAAGGACTTCAAGTTGGG+GGG	0.650449	1.3:+1652814	None:intergenic
GATAAGAGGAAGGAACGCGC+AGG	0.659068	1.3:-1652911	MS.gene90754:CDS
ACACAAGACTAAGCGTGGAG+AGG	0.667676	1.3:-1653307	MS.gene90754:CDS
GAGCAAGGACTTCAAGTTGG+GGG	0.688692	1.3:+1652813	None:intergenic
GAGGTTGGATGGAACTACGG+TGG	0.706495	1.3:-1653045	MS.gene90754:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTGTAAATTATACAATAAAA+TGG	+	chr1.3:1652925-1652944	None:intergenic	15.0%
!!!	CTTTTTGTTGTTGAATTAAA+AGG	-	chr1.3:1652951-1652970	MS.gene90754:intron	20.0%
!!!	GTGATTTTTTTTGTTTTGTT+TGG	-	chr1.3:1653102-1653121	MS.gene90754:CDS	20.0%
!!!	GTGGTTTTGAATTTTTTAAT+TGG	-	chr1.3:1653157-1653176	MS.gene90754:intron	20.0%
!!!	TTAATTGGTTTCTTGTTTAT+AGG	-	chr1.3:1653172-1653191	MS.gene90754:intron	20.0%
!	TGAATGAGTGAATGAAATTA+GGG	-	chr1.3:1652993-1653012	MS.gene90754:intron	25.0%
!!	GAATTTGATCAACAATTTTG+TGG	-	chr1.3:1653138-1653157	MS.gene90754:intron	25.0%
	CTGAATGAGTGAATGAAATT+AGG	-	chr1.3:1652992-1653011	MS.gene90754:intron	30.0%
	AGAAGCTGCTCAATAAATTG+AGG	-	chr1.3:1653243-1653262	MS.gene90754:CDS	35.0%
	ATATGACAAGATCAAGAGGA+TGG	-	chr1.3:1652873-1652892	MS.gene90754:CDS	35.0%
	ATCCTCTTGATCTTGTCATA+TGG	+	chr1.3:1652874-1652893	None:intergenic	35.0%
	GAAGCTGCTCAATAAATTGA+GGG	-	chr1.3:1653244-1653263	MS.gene90754:CDS	35.0%
	CTCCATATGACAAGATCAAG+AGG	-	chr1.3:1652869-1652888	MS.gene90754:CDS	40.0%
	CTCTTGATCTTGTCATATGG+AGG	+	chr1.3:1652871-1652890	None:intergenic	40.0%
	TTGATCTTGTCATATGGAGG+AGG	+	chr1.3:1652868-1652887	None:intergenic	40.0%
!	GGTTTCTTGTTTATAGGAGC+TGG	-	chr1.3:1653178-1653197	MS.gene90754:intron	40.0%
!	TGGTTCTTACTTGAGAGCAT+CGG	+	chr1.3:1652905-1652924	None:intergenic	40.0%
!	TTCTTGTTTATAGGAGCTGG+AGG	-	chr1.3:1653181-1653200	MS.gene90754:intron	40.0%
!!	AGGGTGTTGAGACTTCAAAA+GGG	-	chr1.3:1653012-1653031	MS.gene90754:intron	40.0%
!!	TAGGGTGTTGAGACTTCAAA+AGG	-	chr1.3:1653011-1653030	MS.gene90754:intron	40.0%
	AGTTGTCATCAGAGGTTGGA+TGG	-	chr1.3:1653058-1653077	MS.gene90754:CDS	45.0%
	ATACCACACAAGACTAAGCG+TGG	-	chr1.3:1652802-1652821	MS.gene90754:CDS	45.0%
	GGACACAAATACTGCTTGCT+CGG	-	chr1.3:1653033-1653052	MS.gene90754:intron	45.0%
	TATAGGAGCTGGAGGATAAG+AGG	-	chr1.3:1653189-1653208	MS.gene90754:intron	45.0%
	TCAGAGGTTGGATGGAACTA+CGG	-	chr1.3:1653066-1653085	MS.gene90754:CDS	45.0%
!!	GGTTCTTACTTGAGAGCATC+GGG	+	chr1.3:1652904-1652923	None:intergenic	45.0%
	ACACAAGACTAAGCGTGGAG+AGG	-	chr1.3:1652807-1652826	MS.gene90754:CDS	50.0%
	GAACTACGGTGGCATCATCA+AGG	-	chr1.3:1653080-1653099	MS.gene90754:CDS	50.0%
	GCGCGATTGAAGGTCTATGA+GGG	-	chr1.3:1652838-1652857	MS.gene90754:CDS	50.0%
	GGACAGTTGTCATCAGAGGT+TGG	-	chr1.3:1653054-1653073	MS.gene90754:CDS	50.0%
!	GGGAGCAAGGACTTCAAGTT+GGG	+	chr1.3:1653306-1653325	None:intergenic	50.0%
!!	AGCAAGGACTTCAAGTTGGG+GGG	+	chr1.3:1653303-1653322	None:intergenic	50.0%
!!	GAGCAAGGACTTCAAGTTGG+GGG	+	chr1.3:1653304-1653323	None:intergenic	50.0%
!!	GGAGCAAGGACTTCAAGTTG+GGG	+	chr1.3:1653305-1653324	None:intergenic	50.0%
!!	TCTCCACGCTTAGTCTTGTG+TGG	+	chr1.3:1652808-1652827	None:intergenic	50.0%
	CGCGCGATTGAAGGTCTATG+AGG	-	chr1.3:1652837-1652856	MS.gene90754:CDS	55.0%
	CGGGAGCAAGGACTTCAAGT+TGG	+	chr1.3:1653307-1653326	None:intergenic	55.0%
	GAGGTTGGATGGAACTACGG+TGG	-	chr1.3:1653069-1653088	MS.gene90754:CDS	55.0%
	GATAAGAGGAAGGAACGCGC+AGG	-	chr1.3:1653203-1653222	MS.gene90754:intron	55.0%
	GCTCGGACAGTTGTCATCAG+AGG	-	chr1.3:1653050-1653069	MS.gene90754:CDS	55.0%
	GGAGCTGGAGGATAAGAGGA+AGG	-	chr1.3:1653193-1653212	MS.gene90754:intron	55.0%
	GAGGAAGGAACGCGCAGGTT+TGG	-	chr1.3:1653208-1653227	MS.gene90754:intron	60.0%
	GGCTGCACTCGCGCGATTGA+AGG	-	chr1.3:1652828-1652847	MS.gene90754:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	1652799	1653337	1652799	ID=MS.gene90754
chr1.3	mRNA	1652799	1653337	1652799	ID=MS.gene90754.t1;Parent=MS.gene90754
chr1.3	exon	1653222	1653337	1653222	ID=MS.gene90754.t1.exon1;Parent=MS.gene90754.t1
chr1.3	CDS	1653222	1653337	1653222	ID=cds.MS.gene90754.t1;Parent=MS.gene90754.t1
chr1.3	exon	1653035	1653122	1653035	ID=MS.gene90754.t1.exon2;Parent=MS.gene90754.t1
chr1.3	CDS	1653035	1653122	1653035	ID=cds.MS.gene90754.t1;Parent=MS.gene90754.t1
chr1.3	exon	1652799	1652942	1652799	ID=MS.gene90754.t1.exon3;Parent=MS.gene90754.t1
chr1.3	CDS	1652799	1652942	1652799	ID=cds.MS.gene90754.t1;Parent=MS.gene90754.t1

Gene Sequence

>MS.gene90754

ATGATACCACACAAGACTAAGCGTGGAGAGGCTGCACTCGCGCGATTGAAGGTCTATGAGGGTATTCCTCCTCCATATGACAAGATCAAGAGGATGGTTGTTCCCGATGCTCTCAAGTAAGAACCATTTTATTGTATAATTTACAGTTTTGACTTTTTGTTGTTGAATTAAAAGGATTTATTGAGTTTGAATACTGAATGAGTGAATGAAATTAGGGTGTTGAGACTTCAAAAGGGACACAAATACTGCTTGCTCGGACAGTTGTCATCAGAGGTTGGATGGAACTACGGTGGCATCATCAAGGTGATTTTTTTTGTTTTGTTTGGAGTGTTTTGCTTTGAATTTGATCAACAATTTTGTGGTTTTGAATTTTTTAATTGGTTTCTTGTTTATAGGAGCTGGAGGATAAGAGGAAGGAACGCGCAGGTTTGGCTTACGAGAAAAAGAAGCTGCTCAATAAATTGAGGGCTAAAGCTGAGAAGATTGTAGACGAGAAGCTTGCCCCCCAACTTGAAGTCCTTGCTCCCGTTAAGTACTGA

Protein sequence

>MS.gene90754.t1

MIPHKTKRGEAALARLKVYEGIPPPYDKIKRMVVPDALKVLRLQKGHKYCLLGQLSSEVGWNYGGIIKELEDKRKERAGLAYEKKKLLNKLRAKAEKIVDEKLAPQLEVLAPVKY