AlfalfaGEDB | MS.gene056917

M. sativa cultivar XinJiangDaYe / MS.gene056917

KEGG: K02962
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00833.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056917.t1	XP_013467927.1	97.9	145	2	1	1	145	1	144	1.10E-68	269.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056917.t1	P49215	83.1	148	18	3	1	145	1	144	9.6e-58	224.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056917.t1	I3SPL5	97.9	145	2	1	1	145	1	144	7.8e-69	269.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049876	MS.gene056917	0.804091	2.55E-49	-1.69E-46
MS.gene050290	MS.gene056917	0.864474	1.19E-64	-1.69E-46
MS.gene050791	MS.gene056917	0.834039	3.77E-56	-1.69E-46
MS.gene051904	MS.gene056917	0.804268	2.34E-49	-1.69E-46
MS.gene054937	MS.gene056917	0.874674	5.62E-68	-1.69E-46
MS.gene056075	MS.gene056917	0.803989	2.68E-49	-1.69E-46
MS.gene056257	MS.gene056917	0.804681	1.92E-49	-1.69E-46
MS.gene056917	MS.gene057092	0.851322	9.57E-61	-1.69E-46
MS.gene056917	MS.gene058354	0.816246	6.10E-52	-1.69E-46
MS.gene056917	MS.gene065715	0.892761	1.18E-74	-1.69E-46
MS.gene056917	MS.gene06581	0.849777	2.60E-60	-1.69E-46
MS.gene056917	MS.gene06583	0.836359	9.80E-57	-1.69E-46
MS.gene056917	MS.gene06803	0.802819	4.68E-49	-1.69E-46
MS.gene056917	MS.gene068255	0.811483	6.84E-51	-1.69E-46
MS.gene056917	MS.gene069313	0.835266	1.86E-56	-1.69E-46
MS.gene056917	MS.gene07018	0.810123	1.35E-50	-1.69E-46
MS.gene056917	MS.gene071364	0.906157	2.00E-80	-1.69E-46
MS.gene056917	MS.gene071366	0.89799	8.22E-77	-1.69E-46
MS.gene056917	MS.gene071958	0.80845	3.08E-50	-1.69E-46
MS.gene056917	MS.gene072589	0.833433	5.35E-56	-1.69E-46
MS.gene056917	MS.gene072591	0.83965	1.40E-57	-1.69E-46
MS.gene056917	MS.gene072769	0.817759	2.79E-52	-1.69E-46
MS.gene056917	MS.gene073078	0.831711	1.43E-55	-1.69E-46
MS.gene056917	MS.gene074191	0.800932	1.14E-48	-1.69E-46
MS.gene056917	MS.gene074594	0.814491	1.50E-51	-1.69E-46
MS.gene056917	MS.gene08343	0.82225	2.62E-53	-1.69E-46
MS.gene056917	MS.gene21636	0.809806	1.58E-50	-1.69E-46
MS.gene056917	MS.gene22784	0.862176	6.10E-64	-1.69E-46
MS.gene056917	MS.gene23126	0.811052	8.49E-51	-1.69E-46
MS.gene056917	MS.gene23437	0.84606	2.75E-59	-1.69E-46
MS.gene056917	MS.gene24639	0.810971	8.84E-51	-1.69E-46
MS.gene056917	MS.gene24706	0.862974	3.47E-64	-1.69E-46
MS.gene056917	MS.gene31177	0.888743	4.50E-73	-1.69E-46
MS.gene056917	MS.gene31991	0.849802	2.56E-60	-1.69E-46
MS.gene056917	MS.gene37037	0.812103	5.02E-51	-1.69E-46
MS.gene056917	MS.gene40831	0.851033	1.16E-60	-1.69E-46
MS.gene056917	MS.gene43070	0.865214	6.94E-65	-1.69E-46
MS.gene056917	MS.gene43071	0.869933	2.13E-66	-1.69E-46
MS.gene056917	MS.gene45470	0.890168	1.26E-73	-1.69E-46
MS.gene056917	MS.gene46387	0.812901	3.36E-51	-1.69E-46
MS.gene056917	MS.gene49109	0.805711	1.17E-49	-1.69E-46
MS.gene056917	MS.gene50252	0.839665	1.38E-57	-1.69E-46
MS.gene056917	MS.gene51562	0.816948	4.25E-52	-1.69E-46
MS.gene056917	MS.gene56189	0.804358	2.24E-49	-1.69E-46
MS.gene056917	MS.gene57391	0.827764	1.31E-54	-1.69E-46
MS.gene056917	MS.gene58215	0.864892	8.77E-65	-1.69E-46
MS.gene056917	MS.gene58448	0.820064	8.36E-53	-1.69E-46
MS.gene056917	MS.gene58776	0.870267	1.66E-66	-1.69E-46
MS.gene056917	MS.gene65076	0.825243	5.21E-54	-1.69E-46
MS.gene056917	MS.gene70215	0.827918	1.20E-54	-1.69E-46
MS.gene056917	MS.gene72382	0.802428	5.64E-49	-1.69E-46
MS.gene056917	MS.gene77160	0.850405	1.73E-60	-1.69E-46
MS.gene056917	MS.gene77163	0.829085	6.27E-55	-1.69E-46
MS.gene056917	MS.gene77282	0.835322	1.79E-56	-1.69E-46
MS.gene056917	MS.gene81099	0.919171	6.28E-87	-1.69E-46
MS.gene056917	MS.gene81493	0.813935	1.99E-51	-1.69E-46
MS.gene056917	MS.gene83961	0.816672	4.90E-52	-1.69E-46
MS.gene056917	MS.gene84316	0.823401	1.41E-53	-1.69E-46
MS.gene056917	MS.gene87309	0.856531	3.02E-62	-1.69E-46
MS.gene056917	MS.gene88892	0.803798	2.94E-49	-1.69E-46
MS.gene056917	MS.gene90723	0.812661	3.79E-51	-1.69E-46
MS.gene056917	MS.gene90754	0.82607	3.32E-54	-1.69E-46
MS.gene056917	MS.gene90833	0.802085	6.63E-49	-1.69E-46
MS.gene056917	MS.gene91408	0.822214	2.67E-53	-1.69E-46
MS.gene056917	MS.gene91826	0.867315	1.50E-65	-1.69E-46
MS.gene056917	MS.gene95828	0.865756	4.69E-65	-1.69E-46
MS.gene056917	MS.gene96225	0.821582	3.74E-53	-1.69E-46
MS.gene056917	MS.gene99599	0.828329	9.55E-55	-1.69E-46
MS.gene056917	MS.gene99744	0.812536	4.04E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene056917	MS.gene023275	PPI
MS.gene08344	MS.gene056917	PPI
MS.gene08343	MS.gene056917	PPI
MS.gene028176	MS.gene056917	PPI
MS.gene056917	MS.gene028176	PPI
MS.gene056917	MS.gene039072	PPI
MS.gene056917	MS.gene040536	PPI
MS.gene056917	MS.gene54136	PPI
MS.gene056917	MS.gene34957	PPI
MS.gene034832	MS.gene056917	PPI
MS.gene34958	MS.gene056917	PPI
MS.gene065715	MS.gene056917	PPI
MS.gene056917	MS.gene023274	PPI
MS.gene034834	MS.gene056917	PPI
MS.gene028177	MS.gene056917	PPI
MS.gene039073	MS.gene056917	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056917.t1	MTR_1g058250	97.931	145	2	1	1	145	1	144	2.85e-100	284

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056917.t1	AT5G04800	80.420	143	21	2	1	143	1	136	4.35e-77	225
MS.gene056917.t1	AT5G04800	80.420	143	21	2	1	143	1	136	4.35e-77	225
MS.gene056917.t1	AT5G04800	80.420	143	21	2	1	143	1	136	4.35e-77	225
MS.gene056917.t1	AT5G04800	80.420	143	21	2	1	143	1	136	4.35e-77	225
MS.gene056917.t1	AT2G05220	79.021	143	22	2	1	143	1	135	2.81e-75	221
MS.gene056917.t1	AT2G05220	79.021	143	22	2	1	143	1	135	2.81e-75	221
MS.gene056917.t1	AT3G10610	80.576	139	20	2	1	135	1	136	8.41e-75	219
MS.gene056917.t1	AT2G04390	79.021	143	23	2	1	143	1	136	1.98e-74	218

Find 41 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGAGATTCCCGGGGTTGTTC+AGG	0.315547	1.1:+45379469	MS.gene056917:CDS
TTTGAATCCTCTTCATCAAA+TGG	0.339004	1.1:-45379298	None:intergenic
AGTAGTAACGTTCGATTACT+TGG	0.348773	1.1:-45379175	None:intergenic
TTGTTGCGAAGACGCTTTGA+TGG	0.348895	1.1:-45379257	None:intergenic
CCAAACGGAACCTGCTGAAC+AGG	0.358474	1.1:-45379503	None:intergenic
CTTGATATGCTTGCTGCTCT+TGG	0.359734	1.1:+45379440	MS.gene056917:CDS
CTTGGTATGTCCGAGATTCC+CGG	0.369339	1.1:+45379458	MS.gene056917:CDS
GGTGTTGTTGCTGCTGGTGG+TGG	0.407972	1.1:+45379536	MS.gene056917:CDS
TCTCCTTATCAACCTCAATA+TGG	0.414231	1.1:-45379415	None:intergenic
TTGGTATGTCCGAGATTCCC+GGG	0.419949	1.1:+45379459	MS.gene056917:CDS
TTGCTGCTGGTGGTGGAAGA+AGG	0.424565	1.1:+45379543	MS.gene056917:CDS
GAATCAATGCAACTTCTTCA+AGG	0.430500	1.1:-45379235	None:intergenic
TTGAATCCTCTTCATCAAAT+GGG	0.440060	1.1:-45379297	None:intergenic
TTTGATGAAGAGGATTCAAA+AGG	0.440304	1.1:+45379301	MS.gene056917:CDS
CCTGTTCAGCAGGTTCCGTT+TGG	0.441092	1.1:+45379503	MS.gene056917:CDS
CAGCAGGTTCCGTTTGGAAG+AGG	0.445053	1.1:+45379509	MS.gene056917:CDS
GGTGCTGGTGTTGTTGCTGC+TGG	0.451152	1.1:+45379530	MS.gene056917:CDS
TGAACCTGTTCCTGTTCAGC+AGG	0.453615	1.1:+45379493	MS.gene056917:CDS
GGAAGAGCGTGAGAGGAGGA+TGG	0.458231	1.1:+45379358	MS.gene056917:CDS
TTTGTTGGTGTGGAAATCTA+GGG	0.464737	1.1:-45379207	None:intergenic
GTTCCGTTTGGAAGAGGTGC+TGG	0.465201	1.1:+45379515	MS.gene056917:CDS
GGAACCTGCTGAACAGGAAC+AGG	0.466414	1.1:-45379497	None:intergenic
TGACCATATTGAGGTTGATA+AGG	0.472007	1.1:+45379412	MS.gene056917:CDS
CTGAACAACCCCGGGAATCT+CGG	0.484648	1.1:-45379468	None:intergenic
ATTCAAAAGGGACCGGTTCG+TGG	0.491412	1.1:+45379314	MS.gene056917:CDS
TTTCAACCCATTTGATGAAG+AGG	0.506012	1.1:+45379291	MS.gene056917:CDS
GCTGGTGTTGTTGCTGCTGG+TGG	0.527362	1.1:+45379533	MS.gene056917:CDS
CGTCTTCGCAACAAGATCGC+TGG	0.529414	1.1:+45379266	MS.gene056917:CDS
TTCAACGAGATTCCACGAAC+CGG	0.549035	1.1:-45379326	None:intergenic
TACAAGAGGAAGAGCGTGAG+AGG	0.560260	1.1:+45379351	MS.gene056917:CDS
ACACCAGCACCTCTTCCAAA+CGG	0.569681	1.1:-45379518	None:intergenic
TTGATGAAGAGGATTCAAAA+GGG	0.572781	1.1:+45379302	MS.gene056917:CDS
GAAGAGGATTCAAAAGGGAC+CGG	0.577199	1.1:+45379307	MS.gene056917:CDS
GTTCTGATAGCAGAAACATC+AGG	0.577897	1.1:-45379389	None:intergenic
TATCAGAACTGACCATATTG+AGG	0.583233	1.1:+45379403	MS.gene056917:CDS
AAGAGGAAGAGCGTGAGAGG+AGG	0.595347	1.1:+45379354	MS.gene056917:CDS
AGGTTCAGCCTGAACAACCC+CGG	0.604387	1.1:-45379477	None:intergenic
GGTTCAGCCTGAACAACCCC+GGG	0.607828	1.1:-45379476	None:intergenic
TGGTATGTCCGAGATTCCCG+GGG	0.623039	1.1:+45379460	MS.gene056917:CDS
ACGTTCGATTACTTGGCGTG+AGG	0.642792	1.1:-45379168	None:intergenic
AATCTCGTTGAAGCTACAAG+AGG	0.693243	1.1:+45379337	MS.gene056917:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTTCAAGGATTTTTTTGT+TGG	-	chr1.1:45379225-45379244	None:intergenic	25.0%
	TTGAATCCTCTTCATCAAAT+GGG	-	chr1.1:45379300-45379319	None:intergenic	30.0%
	TTGATGAAGAGGATTCAAAA+GGG	+	chr1.1:45379302-45379321	MS.gene056917:CDS	30.0%
	TTTGAATCCTCTTCATCAAA+TGG	-	chr1.1:45379301-45379320	None:intergenic	30.0%
	TTTGATGAAGAGGATTCAAA+AGG	+	chr1.1:45379301-45379320	MS.gene056917:CDS	30.0%
	AGTAGTAACGTTCGATTACT+TGG	-	chr1.1:45379178-45379197	None:intergenic	35.0%
	GAATCAATGCAACTTCTTCA+AGG	-	chr1.1:45379238-45379257	None:intergenic	35.0%
	TATCAGAACTGACCATATTG+AGG	+	chr1.1:45379403-45379422	MS.gene056917:CDS	35.0%
	TCTCCTTATCAACCTCAATA+TGG	-	chr1.1:45379418-45379437	None:intergenic	35.0%
	TTTCAACCCATTTGATGAAG+AGG	+	chr1.1:45379291-45379310	MS.gene056917:CDS	35.0%
	TTTGTTGGTGTGGAAATCTA+GGG	-	chr1.1:45379210-45379229	None:intergenic	35.0%
!	TTTTGTTGGTGTGGAAATCT+AGG	-	chr1.1:45379211-45379230	None:intergenic	35.0%
!!	TGACCATATTGAGGTTGATA+AGG	+	chr1.1:45379412-45379431	MS.gene056917:CDS	35.0%
!!!	CAAGGATTTTTTTGTTGGTG+TGG	-	chr1.1:45379220-45379239	None:intergenic	35.0%
!!!	TGAGGATTTTTTCACGGTTT+TGG	-	chr1.1:45379153-45379172	None:intergenic	35.0%
	AATCTCGTTGAAGCTACAAG+AGG	+	chr1.1:45379337-45379356	MS.gene056917:CDS	40.0%
	GTTCTGATAGCAGAAACATC+AGG	-	chr1.1:45379392-45379411	None:intergenic	40.0%
!!!	TTGGCGTGAGGATTTTTTCA+CGG	-	chr1.1:45379159-45379178	None:intergenic	40.0%
	GAAGAGGATTCAAAAGGGAC+CGG	+	chr1.1:45379307-45379326	MS.gene056917:CDS	45.0%
	TTCAACGAGATTCCACGAAC+CGG	-	chr1.1:45379329-45379348	None:intergenic	45.0%
	TTGTTGCGAAGACGCTTTGA+TGG	-	chr1.1:45379260-45379279	None:intergenic	45.0%
!	CTTGATATGCTTGCTGCTCT+TGG	+	chr1.1:45379440-45379459	MS.gene056917:CDS	45.0%
	ACACCAGCACCTCTTCCAAA+CGG	-	chr1.1:45379521-45379540	None:intergenic	50.0%
	ACGTTCGATTACTTGGCGTG+AGG	-	chr1.1:45379171-45379190	None:intergenic	50.0%
	CTTGGTATGTCCGAGATTCC+CGG	+	chr1.1:45379458-45379477	MS.gene056917:CDS	50.0%
	TGAACCTGTTCCTGTTCAGC+AGG	+	chr1.1:45379493-45379512	MS.gene056917:CDS	50.0%
	TTGGTATGTCCGAGATTCCC+GGG	+	chr1.1:45379459-45379478	MS.gene056917:CDS	50.0%
!	TACAAGAGGAAGAGCGTGAG+AGG	+	chr1.1:45379351-45379370	MS.gene056917:CDS	50.0%
!!	ATTCAAAAGGGACCGGTTCG+TGG	+	chr1.1:45379314-45379333	MS.gene056917:CDS	50.0%
	AGGTTCAGCCTGAACAACCC+CGG	-	chr1.1:45379480-45379499	None:intergenic	55.0%
	CAGCAGGTTCCGTTTGGAAG+AGG	+	chr1.1:45379509-45379528	MS.gene056917:CDS	55.0%
	CCAAACGGAACCTGCTGAAC+AGG	-	chr1.1:45379506-45379525	None:intergenic	55.0%
	CCTGTTCAGCAGGTTCCGTT+TGG	+	chr1.1:45379503-45379522	MS.gene056917:CDS	55.0%
	CGTCTTCGCAACAAGATCGC+TGG	+	chr1.1:45379266-45379285	MS.gene056917:CDS	55.0%
	CTGAACAACCCCGGGAATCT+CGG	-	chr1.1:45379471-45379490	None:intergenic	55.0%
	GGAACCTGCTGAACAGGAAC+AGG	-	chr1.1:45379500-45379519	None:intergenic	55.0%
	TGGTATGTCCGAGATTCCCG+GGG	+	chr1.1:45379460-45379479	MS.gene056917:CDS	55.0%
!	AAGAGGAAGAGCGTGAGAGG+AGG	+	chr1.1:45379354-45379373	MS.gene056917:CDS	55.0%
!	GTTCCGTTTGGAAGAGGTGC+TGG	+	chr1.1:45379515-45379534	MS.gene056917:CDS	55.0%
!!	TTGCTGCTGGTGGTGGAAGA+AGG	+	chr1.1:45379543-45379562	MS.gene056917:CDS	55.0%
	CGAGATTCCCGGGGTTGTTC+AGG	+	chr1.1:45379469-45379488	MS.gene056917:CDS	60.0%
	GGTTCAGCCTGAACAACCCC+GGG	-	chr1.1:45379479-45379498	None:intergenic	60.0%
!	GGAAGAGCGTGAGAGGAGGA+TGG	+	chr1.1:45379358-45379377	MS.gene056917:CDS	60.0%
!!	GCTGGTGTTGTTGCTGCTGG+TGG	+	chr1.1:45379533-45379552	MS.gene056917:CDS	60.0%
!!	GGTGCTGGTGTTGTTGCTGC+TGG	+	chr1.1:45379530-45379549	MS.gene056917:CDS	60.0%
!!	GGTGTTGTTGCTGCTGGTGG+TGG	+	chr1.1:45379536-45379555	MS.gene056917:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	45379134	45379571	45379134	ID=MS.gene056917
chr1.1	mRNA	45379134	45379571	45379134	ID=MS.gene056917.t1;Parent=MS.gene056917
chr1.1	exon	45379134	45379571	45379134	ID=MS.gene056917.t1.exon1;Parent=MS.gene056917.t1
chr1.1	CDS	45379134	45379571	45379134	ID=cds.MS.gene056917.t1;Parent=MS.gene056917.t1

Gene Sequence

>MS.gene056917

ATGGGTCGTGTCAGAACCAAAACCGTGAAAAAATCCTCACGCCAAGTAATCGAACGTTACTACTCAAAAATGACCCTAGATTTCCACACCAACAAAAAAATCCTTGAAGAAGTTGCATTGATTCCATCAAAGCGTCTTCGCAACAAGATCGCTGGATTTTCAACCCATTTGATGAAGAGGATTCAAAAGGGACCGGTTCGTGGAATCTCGTTGAAGCTACAAGAGGAAGAGCGTGAGAGGAGGATGGATTTCGTTCCTGATGTTTCTGCTATCAGAACTGACCATATTGAGGTTGATAAGGAGACTCTTGATATGCTTGCTGCTCTTGGTATGTCCGAGATTCCCGGGGTTGTTCAGGCTGAACCTGTTCCTGTTCAGCAGGTTCCGTTTGGAAGAGGTGCTGGTGTTGTTGCTGCTGGTGGTGGAAGAAGGTTTTGA

Protein sequence

>MS.gene056917.t1

MGRVRTKTVKKSSRQVIERYYSKMTLDFHTNKKILEEVALIPSKRLRNKIAGFSTHLMKRIQKGPVRGISLKLQEEERERRMDFVPDVSAIRTDHIEVDKETLDMLAALGMSEIPGVVQAEPVPVQQVPFGRGAGVVAAGGGRRF