AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91408


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91408.t1 MTR_4g094598 100.000 130 0 0 1 130 1 130 5.74e-94 267
MS.gene91408.t1 MTR_1g105415 100.000 130 0 0 1 130 1 130 5.74e-94 267
MS.gene91408.t1 MTR_4g117470 99.231 130 1 0 1 130 1 130 2.82e-93 265
MS.gene91408.t1 MTR_8g101910 52.000 125 60 0 6 130 5 129 9.74e-46 145
MS.gene91408.t1 MTR_8g101910 49.474 95 48 0 6 100 5 99 9.43e-29 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91408.t1 AT5G59850 98.462 130 2 0 1 130 1 130 4.89e-93 265
MS.gene91408.t1 AT1G07770 98.462 130 2 0 1 130 1 130 4.89e-93 265
MS.gene91408.t1 AT1G07770 98.462 130 2 0 1 130 1 130 4.89e-93 265
MS.gene91408.t1 AT1G07770 98.462 130 2 0 1 130 1 130 4.89e-93 265
MS.gene91408.t1 AT3G46040 96.923 130 4 0 1 130 1 130 1.11e-91 261
MS.gene91408.t1 AT2G39590 91.538 130 11 0 1 130 1 130 4.60e-87 249
MS.gene91408.t1 AT4G29430 54.400 125 57 0 6 130 5 129 1.51e-47 149
MS.gene91408.t1 AT2G19720 53.600 125 58 0 6 130 5 129 1.71e-45 144

Find 26 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGATTATCAAATTCCTTA+TGG 0.311584 1.3:-71854578 MS.gene91408:CDS
TTGTGTTTGTAGGATACATT+GGG 0.378729 1.3:-71854435 MS.gene91408:intron
GACTACCTCTGCTGGTATCA+TGG 0.414748 1.3:-71853095 MS.gene91408:CDS
ACCACCAACATTCTTTCTCC+TGG 0.414976 1.3:+71853060 None:intergenic
GAAAGCGTCAAGTTATGATT+AGG 0.452540 1.3:-71854612 MS.gene91408:CDS
TTTGTGTTTGTAGGATACAT+TGG 0.464282 1.3:-71854436 MS.gene91408:intron
AATGTTGGTGGTAAAGTGTT+GGG 0.506719 1.3:-71853049 MS.gene91408:CDS
TTGAATATGTCGATGATCAC+AGG 0.509301 1.3:-71854408 MS.gene91408:CDS
AAAATTGTCGTCGAATTGAA+TGG 0.519692 1.3:-71854379 MS.gene91408:CDS
AGATTGAAGGATGGACTGCT+AGG 0.524175 1.3:-71854303 MS.gene91408:CDS
ATTGTCTTGACTACCTCTGC+TGG 0.534592 1.3:-71853103 MS.gene91408:CDS
GAATGTTGGTGGTAAAGTGT+TGG 0.537027 1.3:-71853050 MS.gene91408:CDS
TATGTCGATGATCACAGGTC+TGG 0.547616 1.3:-71854403 MS.gene91408:CDS
TCATGGATCATGAAGAGGCC+AGG 0.549802 1.3:-71853078 MS.gene91408:CDS
TAGGCTTCTTCCCTCAAGAC+AGG 0.576488 1.3:-71854284 MS.gene91408:intron
GCCAGGAGAAAGAATGTTGG+TGG 0.584787 1.3:-71853061 MS.gene91408:CDS
GTTGGTGTCAAAGAGATTGA+AGG 0.610557 1.3:-71854316 MS.gene91408:CDS
CTTATGGTCATGCAAAAGCA+TGG 0.625786 1.3:-71854562 MS.gene91408:intron
ATGATCCATGATACCAGCAG+AGG 0.644078 1.3:+71853090 None:intergenic
TGTGTTTGTAGGATACATTG+GGG 0.648921 1.3:-71854434 MS.gene91408:intron
TGGTATCATGGATCATGAAG+AGG 0.653772 1.3:-71853083 MS.gene91408:CDS
GAGGCCAGGAGAAAGAATGT+TGG 0.655684 1.3:-71853064 MS.gene91408:CDS
AATGGTAGATTGAACAAGTG+TGG 0.659687 1.3:-71854361 MS.gene91408:CDS
GTGTCAAAGAGATTGAAGGA+TGG 0.661917 1.3:-71854312 MS.gene91408:CDS
ATGTACAATGCTGAGAAACG+TGG 0.686253 1.3:-71854634 MS.gene91408:CDS
TTGACACCAACATCAAAACG+AGG 0.729538 1.3:+71854328 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAAAATATTAAAAGAT+GGG + chr1.3:71854381-71854400 None:intergenic 10.0%
!! TAAATGAAAATATTAAAAGA+TGG + chr1.3:71854382-71854401 None:intergenic 10.0%
!! CTATAAAACAAACTTAAATA+AGG + chr1.3:71853776-71853795 None:intergenic 15.0%
!! TAAACATTACAATTGAATAA+TGG + chr1.3:71854433-71854452 None:intergenic 15.0%
!! AAAATATTAAAAGATGGGAT+AGG + chr1.3:71854376-71854395 None:intergenic 20.0%
!! ATTAGAAATGATTCATGATT+AGG + chr1.3:71854149-71854168 None:intergenic 20.0%
!! ATTTATTGTTTGATTCATGT+AGG - chr1.3:71853189-71853208 MS.gene91408:intron 20.0%
!! CAAATTTATAAGACAAAACT+CGG + chr1.3:71853463-71853482 None:intergenic 20.0%
!! TCAAATAAGTTAATCCAAAT+AGG + chr1.3:71853567-71853586 None:intergenic 20.0%
!! TGTAATGTTTATATACTTGT+CGG - chr1.3:71854442-71854461 MS.gene91408:intron 20.0%
!!! CTTTCTAATTTATTTGCTTT+TGG - chr1.3:71853920-71853939 MS.gene91408:intron 20.0%
!!! TCAAACTACTAACATTTTTA+TGG + chr1.3:71853496-71853515 None:intergenic 20.0%
!!! TGATAGTTTAATTTACATGA+TGG - chr1.3:71853513-71853532 MS.gene91408:intron 20.0%
! AATTGAATAATGGTTGCAAA+AGG + chr1.3:71854423-71854442 None:intergenic 25.0%
! AGTGATTATCAAATTCCTTA+TGG - chr1.3:71853131-71853150 MS.gene91408:intron 25.0%
! ATAGATGATAGTCAAATAGT+CGG + chr1.3:71854239-71854258 None:intergenic 25.0%
! CCATTAAAGCATAATAAGAA+GGG + chr1.3:71854270-71854289 None:intergenic 25.0%
! CCTTCTTATTATGCTTTAAT+GGG - chr1.3:71854268-71854287 MS.gene91408:intron 25.0%
! CTGACTTATACTTCATATAT+TGG - chr1.3:71854055-71854074 MS.gene91408:intron 25.0%
! CTTCTTATTATGCTTTAATG+GGG - chr1.3:71854269-71854288 MS.gene91408:intron 25.0%
! TAAATTCAAACCGTTCTTTA+TGG - chr1.3:71854178-71854197 MS.gene91408:intron 25.0%
! TGTTCTATTTCCACATAATA+AGG + chr1.3:71853891-71853910 None:intergenic 25.0%
! TTGTTTGTTTCCTTATTATG+TGG - chr1.3:71853878-71853897 MS.gene91408:intron 25.0%
!! TTAGTAGGCTTTTAAATCTT+GGG - chr1.3:71853995-71854014 MS.gene91408:intron 25.0%
!! TTGTATACTTTTGTGTTTGT+AGG - chr1.3:71853264-71853283 MS.gene91408:intron 25.0%
!!! CTAATAGGCTTTTAAAACTT+GGG - chr1.3:71853968-71853987 MS.gene91408:intron 25.0%
!!! GAATTTGATAATCACTTTTG+AGG + chr1.3:71853127-71853146 None:intergenic 25.0%
AAAAAAAGCTGACAAAAGCT+TGG + chr1.3:71853658-71853677 None:intergenic 30.0%
AAAAAAGCTGACAAAAGCTT+GGG + chr1.3:71853657-71853676 None:intergenic 30.0%
AAAATTGTCGTCGAATTGAA+TGG - chr1.3:71853330-71853349 MS.gene91408:intron 30.0%
ACCTTTATATTAGGCCTAAT+AGG - chr1.3:71853953-71853972 MS.gene91408:intron 30.0%
AGAACTTATGAAATCAGCTA+TGG - chr1.3:71853624-71853643 MS.gene91408:intron 30.0%
CCCATTAAAGCATAATAAGA+AGG + chr1.3:71854271-71854290 None:intergenic 30.0%
CCCTTCTTATTATGCTTTAA+TGG - chr1.3:71854267-71854286 MS.gene91408:intron 30.0%
GTTTCATTTGATCTTTCTTG+TGG - chr1.3:71854547-71854566 MS.gene91408:intron 30.0%
TCCTGGAATACCTTTATATT+AGG - chr1.3:71853944-71853963 MS.gene91408:intron 30.0%
TGTTTGATTCATGTAGGATT+AGG - chr1.3:71853195-71853214 MS.gene91408:intron 30.0%
TTGCTAAAATCATGCAGTTT+GGG - chr1.3:71854580-71854599 MS.gene91408:CDS 30.0%
TTGTGTTTGTAGGATACATT+GGG - chr1.3:71853274-71853293 MS.gene91408:intron 30.0%
TTTGTGTTTGTAGGATACAT+TGG - chr1.3:71853273-71853292 MS.gene91408:intron 30.0%
! ATCCATTGACATAAGTTTTG+TGG - chr1.3:71853591-71853610 MS.gene91408:intron 30.0%
! GTTAGTAGGCTTTTAAATCT+TGG - chr1.3:71853994-71854013 MS.gene91408:intron 30.0%
!! CATGATTTTAGCAACAGTTA+GGG + chr1.3:71854574-71854593 None:intergenic 30.0%
!! TCCATTGACATAAGTTTTGT+GGG - chr1.3:71853592-71853611 MS.gene91408:intron 30.0%
!! TTGATAATCACTTTTGAGGA+TGG + chr1.3:71853123-71853142 None:intergenic 30.0%
!! TTTAAATCTTGGGTTGGAAT+TGG - chr1.3:71854005-71854024 MS.gene91408:intron 30.0%
!!! ATTTATTTGCTTTTGGTTCC+TGG - chr1.3:71853927-71853946 MS.gene91408:intron 30.0%
!!! CCAAGTTTTAAAAGCCTATT+AGG + chr1.3:71853970-71853989 None:intergenic 30.0%
!!! CCTAATAGGCTTTTAAAACT+TGG - chr1.3:71853967-71853986 MS.gene91408:intron 30.0%
AATGGTAGATTGAACAAGTG+TGG - chr1.3:71853348-71853367 MS.gene91408:intron 35.0%
ACAAGCAACATAACATTCGA+TGG + chr1.3:71854525-71854544 None:intergenic 35.0%
ATACACCCAACCATAAAGAA+CGG + chr1.3:71854191-71854210 None:intergenic 35.0%
CAGATGTTTCACTCACTTAA+GGG - chr1.3:71854102-71854121 MS.gene91408:intron 35.0%
CTTAAGTGAGTGAAACATCT+GGG + chr1.3:71854103-71854122 None:intergenic 35.0%
CTTATTATGCTTTAATGGGG+TGG - chr1.3:71854272-71854291 MS.gene91408:intron 35.0%
GCCTATTAGGCCTAATATAA+AGG + chr1.3:71853957-71853976 None:intergenic 35.0%
GTTGCTAAAATCATGCAGTT+TGG - chr1.3:71854579-71854598 MS.gene91408:CDS 35.0%
TATAGTTCCTAACTATCGAG+TGG - chr1.3:71853791-71853810 MS.gene91408:intron 35.0%
TCAAACCGTTCTTTATGGTT+GGG - chr1.3:71854183-71854202 MS.gene91408:intron 35.0%
TCCCACAAAACTTATGTCAA+TGG + chr1.3:71853596-71853615 None:intergenic 35.0%
TGTGTTTGTAGGATACATTG+GGG - chr1.3:71853275-71853294 MS.gene91408:intron 35.0%
TTCAAACCGTTCTTTATGGT+TGG - chr1.3:71854182-71854201 MS.gene91408:intron 35.0%
TTGAATATGTCGATGATCAC+AGG - chr1.3:71853301-71853320 MS.gene91408:intron 35.0%
! TAGGCTTTTAAATCTTGGGT+TGG - chr1.3:71853999-71854018 MS.gene91408:intron 35.0%
!! AATGTTGGTGGTAAAGTGTT+GGG - chr1.3:71854660-71854679 MS.gene91408:CDS 35.0%
!! ATAAGTTTTGTGGGACTGTT+TGG - chr1.3:71853601-71853620 MS.gene91408:intron 35.0%
!! ATTAGTCCTCGTTTTGATGT+TGG - chr1.3:71853375-71853394 MS.gene91408:intron 35.0%
!! GAAAGCGTCAAGTTATGATT+AGG - chr1.3:71853097-71853116 MS.gene91408:CDS 35.0%
!! GCATGATTTTAGCAACAGTT+AGG + chr1.3:71854575-71854594 None:intergenic 35.0%
!! GCCTAATATAAAGGTATTCC+AGG + chr1.3:71853948-71853967 None:intergenic 35.0%
!! TAAAACTTGGGTTGGTTAGT+AGG - chr1.3:71853980-71853999 MS.gene91408:intron 35.0%
!!! TAGGCTTTTAAAACTTGGGT+TGG - chr1.3:71853972-71853991 MS.gene91408:intron 35.0%
AATAGTCGGTATGCATAGAG+TGG + chr1.3:71854225-71854244 None:intergenic 40.0%
AGAATGACCACTCGATAGTT+AGG + chr1.3:71853801-71853820 None:intergenic 40.0%
ATAGTCGGTATGCATAGAGT+GGG + chr1.3:71854224-71854243 None:intergenic 40.0%
ATGATATGTGCTTCTGCTAC+AGG - chr1.3:71853750-71853769 MS.gene91408:intron 40.0%
ATGTACAATGCTGAGAAACG+TGG - chr1.3:71853075-71853094 MS.gene91408:CDS 40.0%
CACTCACTTAAGGGACTATT+TGG - chr1.3:71854111-71854130 MS.gene91408:intron 40.0%
CCAGATGTTTCACTCACTTA+AGG - chr1.3:71854101-71854120 MS.gene91408:intron 40.0%
CCTTAAGTGAGTGAAACATC+TGG + chr1.3:71854104-71854123 None:intergenic 40.0%
CTGAAGTTGCTATCATCATC+TGG - chr1.3:71854495-71854514 MS.gene91408:intron 40.0%
CTTATGGTCATGCAAAAGCA+TGG - chr1.3:71853147-71853166 MS.gene91408:intron 40.0%
GGTGGACGTAGAATATTCAT+TGG - chr1.3:71854290-71854309 MS.gene91408:CDS 40.0%
GTGTCAAAGAGATTGAAGGA+TGG - chr1.3:71853397-71853416 MS.gene91408:intron 40.0%
TGGTATCATGGATCATGAAG+AGG - chr1.3:71854626-71854645 MS.gene91408:CDS 40.0%
TTGACACCAACATCAAAACG+AGG + chr1.3:71853384-71853403 None:intergenic 40.0%
! CATGCTTTTGCATGACCATA+AGG + chr1.3:71853149-71853168 None:intergenic 40.0%
! TCATGCAAAAGCATGGTATG+TGG - chr1.3:71853154-71853173 MS.gene91408:intron 40.0%
!! GAATGTTGGTGGTAAAGTGT+TGG - chr1.3:71854659-71854678 MS.gene91408:CDS 40.0%
!! GTAGGATTAGGCTTTGTTAC+TGG - chr1.3:71853207-71853226 MS.gene91408:intron 40.0%
!! GTTGGTGTCAAAGAGATTGA+AGG - chr1.3:71853393-71853412 MS.gene91408:intron 40.0%
!! TTTTAGCTGACCTGTCTTGA+GGG + chr1.3:71853438-71853457 None:intergenic 40.0%
!!! TTTTTAGCTGACCTGTCTTG+AGG + chr1.3:71853439-71853458 None:intergenic 40.0%
AATTGGAGAGGGGTTAACCA+GGG - chr1.3:71854022-71854041 MS.gene91408:intron 45.0%
ACCACCAACATTCTTTCTCC+TGG + chr1.3:71854652-71854671 None:intergenic 45.0%
AGTGCACAATATGCGTCTCT+GGG - chr1.3:71853541-71853560 MS.gene91408:intron 45.0%
ATGATCCATGATACCAGCAG+AGG + chr1.3:71854622-71854641 None:intergenic 45.0%
ATTGTCTTGACTACCTCTGC+TGG - chr1.3:71854606-71854625 MS.gene91408:CDS 45.0%
TAGTGCACAATATGCGTCTC+TGG - chr1.3:71853540-71853559 MS.gene91408:intron 45.0%
TATGTCGATGATCACAGGTC+TGG - chr1.3:71853306-71853325 MS.gene91408:intron 45.0%
TCAAGACTCCAACGTCAATG+TGG + chr1.3:71853832-71853851 None:intergenic 45.0%
! GCAAAAGCATGGTATGTGGT+TGG - chr1.3:71853158-71853177 MS.gene91408:intron 45.0%
!! AGATTGAAGGATGGACTGCT+AGG - chr1.3:71853406-71853425 MS.gene91408:intron 45.0%
!! ATCTTGGGTTGGAATTGGAG+AGG - chr1.3:71854010-71854029 MS.gene91408:intron 45.0%
!! TCTTGGGTTGGAATTGGAGA+GGG - chr1.3:71854011-71854030 MS.gene91408:intron 45.0%
GAATTGGAGAGGGGTTAACC+AGG - chr1.3:71854021-71854040 MS.gene91408:intron 50.0%
GACTACCTCTGCTGGTATCA+TGG - chr1.3:71854614-71854633 MS.gene91408:CDS 50.0%
GAGGCCAGGAGAAAGAATGT+TGG - chr1.3:71854645-71854664 MS.gene91408:CDS 50.0%
GCCAGGAGAAAGAATGTTGG+TGG - chr1.3:71854648-71854667 MS.gene91408:CDS 50.0%
TAGGCTTCTTCCCTCAAGAC+AGG - chr1.3:71853425-71853444 MS.gene91408:intron 50.0%
TATGCGTCTCTGGGCCTATT+TGG - chr1.3:71853550-71853569 MS.gene91408:intron 50.0%
TCATGGATCATGAAGAGGCC+AGG - chr1.3:71854631-71854650 MS.gene91408:CDS 50.0%
TTTGCGCACCACATTGACGT+TGG - chr1.3:71853821-71853840 MS.gene91408:intron 50.0%
!! CTTGGGTTGGAATTGGAGAG+GGG - chr1.3:71854012-71854031 MS.gene91408:intron 50.0%
!! AAGTCAGAGAAGCACTGCCC+TGG + chr1.3:71854042-71854061 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 71853036 71854695 71853036 ID=MS.gene91408
chr1.3 mRNA 71853036 71854695 71853036 ID=MS.gene91408.t1;Parent=MS.gene91408
chr1.3 exon 71854563 71854695 71854563 ID=MS.gene91408.t1.exon1;Parent=MS.gene91408.t1
chr1.3 CDS 71854563 71854695 71854563 ID=cds.MS.gene91408.t1;Parent=MS.gene91408.t1
chr1.3 exon 71854285 71854445 71854285 ID=MS.gene91408.t1.exon2;Parent=MS.gene91408.t1
chr1.3 CDS 71854285 71854445 71854285 ID=cds.MS.gene91408.t1;Parent=MS.gene91408.t1
chr1.3 exon 71853036 71853134 71853036 ID=MS.gene91408.t1.exon3;Parent=MS.gene91408.t1
chr1.3 CDS 71853036 71853134 71853036 ID=cds.MS.gene91408.t1;Parent=MS.gene91408.t1
Gene Sequence

>MS.gene91408

ATGGTGAGGGTTAGTGTTTTGAACGATGCACTCAAGAGCATGTACAATGCTGAGAAACGTGGAAAGCGTCAAGTTATGATTAGGCCATCCTCAAAAGTGATTATCAAATTCCTTATGGTCATGCAAAAGCATGGTATGTGGTTGGTTTAACTTATTTATTGTTTGATTCATGTAGGATTAGGCTTTGTTACTGGTGTTGTTGCTTGTGTATCTTTTAATGTGATAATATTGTATACTTTTGTGTTTGTAGGATACATTGGGGAGTTTGAATATGTCGATGATCACAGGTCTGGCAAAATTGTCGTCGAATTGAATGGTAGATTGAACAAGTGTGGTGTTATTAGTCCTCGTTTTGATGTTGGTGTCAAAGAGATTGAAGGATGGACTGCTAGGCTTCTTCCCTCAAGACAGGTCAGCTAAAAAGCCGAGTTTTGTCTTATAAATTTGATATAAATGACCATAAAAATGTTAGTAGTTTGATAGTTTAATTTACATGATGGCTTTTAGTGCACAATATGCGTCTCTGGGCCTATTTGGATTAACTTATTTGAGCTTATCCATTGACATAAGTTTTGTGGGACTGTTTGGAGAACTTATGAAATCAGCTATGGCAATTTTCCCAAGCTTTTGTCAGCTTTTTTTCATACGTTCTTCAGTATAAATTATAAAAACAGCTTATAGCATATAAAAAAACAATTTAACTTTTTATTCTATATGATATGTGCTTCTGCTACAGGCCTTATTTAAGTTTGTTTTATAGTTCCTAACTATCGAGTGGTCATTCTTTTGCGCACCACATTGACGTTGGAGTCTTGATCTCAAATTTGAGCTTAGTTCATTGTTTGTTTGTTTCCTTATTATGTGGAAATAGAACAAAGTTTTCTCTTTCTAATTTATTTGCTTTTGGTTCCTGGAATACCTTTATATTAGGCCTAATAGGCTTTTAAAACTTGGGTTGGTTAGTAGGCTTTTAAATCTTGGGTTGGAATTGGAGAGGGGTTAACCAGGGCAGTGCTTCTCTGACTTATACTTCATATATTGGAAATCACTGTAGTATGAATTGTCCCAGATGTTTCACTCACTTAAGGGACTATTTGGATAAAATGAAATCCTAATCATGAATCATTTCTAATGACAGCATTTAAATTCAAACCGTTCTTTATGGTTGGGTGTATAATTTTGTCTCCCACTCTATGCATACCGACTATTTGACTATCATCTATCTAGTTTACCCTTCTTATTATGCTTTAATGGGGTGGACGTAGAATATTCATTGGTCTTTTAATTTTTGTACAAACAACTTAAAAACACGACTACTGATTTTACAATATGTGCTACCTATCCCATCTTTTAATATTTTCATTTATATTCAAAATCTATCTTGCCTTTTGCAACCATTATTCAATTGTAATGTTTATATACTTGTCGGTTTATTTTACACGTAGATGCAAACTGAACTCTGAAGTTGCTATCATCATCTGGCAAACCATCGAATGTTATGTTGCTTGTTTGTTTCATTTGATCTTTCTTGTGGTCCCTAACTGTTGCTAAAATCATGCAGTTTGGGTATATTGTCTTGACTACCTCTGCTGGTATCATGGATCATGAAGAGGCCAGGAGAAAGAATGTTGGTGGTAAAGTGTTGGGTTTTTTCTATTAG

Protein sequence

>MS.gene91408.t1

MVRVSVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLMVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY