AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91768

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91768.t1 MTR_2g041640 97.704 871 18 2 1 870 1 870 0.0 1758
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91768.t1 AT1G77550 67.517 862 267 5 10 870 3 852 0.0 1197

Find 215 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCCACTTTCCAATATTT+TGG 0.092480 2.1:-49670804 None:intergenic
CATATTATATCTGCATCTTT+TGG 0.139160 2.1:-49673575 None:intergenic
TATTCCTGTCAGATTGTTTC+TGG 0.155637 2.1:+49674291 MS.gene91768:CDS
CAATTTCGGGGAGTGGGCTT+TGG 0.216553 2.1:+49670057 MS.gene91768:CDS
CAACTTTCACAAGCAATTTC+GGG 0.220240 2.1:+49670044 MS.gene91768:CDS
CACATGATGGGCGTCCTTTA+CGG 0.233516 2.1:+49672001 MS.gene91768:CDS
TCAACTTTCACAAGCAATTT+CGG 0.237496 2.1:+49670043 MS.gene91768:CDS
ACTCATCTGTCTCAACTTAT+TGG 0.250244 2.1:+49674035 MS.gene91768:CDS
CCAAACATTTCTGAACTAAA+TGG 0.271808 2.1:+49670770 MS.gene91768:CDS
GGGAAGTGCCATTGAAATTC+TGG 0.272207 2.1:+49670739 MS.gene91768:CDS
AGTTTCCATGAGTCGGATTA+TGG 0.278974 2.1:-49674162 None:intergenic
TGCACTCGTGACTTTCTTCT+TGG 0.281535 2.1:+49671726 MS.gene91768:CDS
CTGTATCTCTTGCAGTTAAA+TGG 0.290895 2.1:+49674739 MS.gene91768:intron
TTAGTGGCACTTAGCCTATA+TGG 0.294517 2.1:+49669769 MS.gene91768:CDS
AGGTACGTGATGGATGAATT+TGG 0.296880 2.1:+49671259 MS.gene91768:intron
GTCGATATTCCTGGTCCTCT+TGG 0.300317 2.1:+49670716 MS.gene91768:CDS
CAGGTTGTTCAATATACTTC+TGG 0.310081 2.1:-49674199 None:intergenic
TATTTGTGCATCCAAAATAT+TGG 0.310689 2.1:+49670793 MS.gene91768:CDS
ACTTCTTGAATAATCAATTC+AGG 0.312500 2.1:-49669808 None:intergenic
CAAAAGATGCAGATATAATA+TGG 0.314062 2.1:+49673576 MS.gene91768:CDS
AGCTAACAACCAATATTCTT+TGG 0.314596 2.1:+49674408 MS.gene91768:CDS
CGTGACTTTCTTCTTGGTAT+TGG 0.317812 2.1:+49671732 MS.gene91768:CDS
TGAAACTCATTTCACTGTTA+TGG 0.319368 2.1:+49674453 MS.gene91768:CDS
AATAGTGCGGGCCATGTTCC+AGG 0.320213 2.1:-49674111 None:intergenic
TGATGTTATGTTTCTCTCAT+TGG 0.323680 2.1:+49669074 MS.gene91768:CDS
GTTGATGATGATGATGTTTC+GGG 0.325483 2.1:+49668946 MS.gene91768:CDS
AATTTCGGGGAGTGGGCTTT+GGG 0.325758 2.1:+49670058 MS.gene91768:CDS
ACTTACCAATTCTGAAGAAC+TGG 0.326545 2.1:-49669874 None:intergenic
TCAGCTTGATCAGCATTAGA+TGG 0.330080 2.1:-49670112 None:intergenic
ACTTGGATAAAATAGTTCTC+TGG 0.333720 2.1:-49671801 None:intergenic
ATTCTCTTGCTTACGTTCAT+TGG 0.335166 2.1:+49670567 MS.gene91768:CDS
AATAAATCACAAGAATATTA+AGG 0.336524 2.1:+49674588 MS.gene91768:CDS
ACTCGCCGCTTCGGGTTTAC+CGG 0.347515 2.1:+49668161 MS.gene91768:CDS
TCGATATTCCTGGTCCTCTT+GGG 0.347686 2.1:+49670717 MS.gene91768:CDS
TATGGCCAACACGGAATGTT+TGG 0.355349 2.1:+49671691 MS.gene91768:CDS
TCTTAAGGAGTTACCAGAAC+TGG 0.362100 2.1:+49670009 MS.gene91768:CDS
ACATGATGGGCGTCCTTTAC+GGG 0.362567 2.1:+49672002 MS.gene91768:CDS
ATAACCCAGTTCTTCAGAAT+TGG 0.365410 2.1:+49669869 MS.gene91768:CDS
AAGTTATACATCTAATTTCC+AGG 0.366200 2.1:-49675330 None:intergenic
TCTAGTGATGGTGATCTTGC+AGG 0.366268 2.1:+49669980 MS.gene91768:intron
AGACGGCGGTGGAGGGATTC+CGG 0.366662 2.1:-49668180 None:intergenic
TTGTAAGCTTGTTTCCATAT+AGG 0.366689 2.1:-49669783 None:intergenic
GGCTCATCAGGAGTCCATTC+AGG 0.370563 2.1:-49670860 None:intergenic
TCTTGCAGGTATTATTCTTA+AGG 0.373169 2.1:+49669994 MS.gene91768:CDS
CGGAATTCTACTCGCCGCTT+CGG 0.374406 2.1:+49668152 MS.gene91768:CDS
GTTGAAGAAATCACAACCTT+TGG 0.377369 2.1:-49675178 None:intergenic
CTCGCCGCCGTCGAAGGTTT+CGG 0.378738 2.1:-49668220 None:intergenic
CTTTCCCTTTCAGAATTTAG+TGG 0.379886 2.1:+49669753 MS.gene91768:intron
AAAGGTGCCACTCTAAAGTT+TGG 0.383885 2.1:-49671307 None:intergenic
GGAATTCTACTCGCCGCTTC+GGG 0.384701 2.1:+49668153 MS.gene91768:CDS
TGAACAACCTGCTTTATTCC+AGG 0.384791 2.1:+49674211 MS.gene91768:CDS
GATGTGTGTGGATATTGATT+TGG 0.393550 2.1:+49668900 MS.gene91768:CDS
TTGCAAGCTCGTGTACAATC+AGG 0.394641 2.1:-49675117 None:intergenic
TCTTCCTTGTTGATCATGCT+TGG 0.395883 2.1:+49668325 MS.gene91768:CDS
GAAGACTTCGCCAAAGTTCA+CGG 0.397878 2.1:+49668132 MS.gene91768:CDS
GGACTTGCCTGCTAGATTCC+CGG 0.398457 2.1:+49669095 MS.gene91768:CDS
TGTTGATGATGATGATGTTT+CGG 0.400062 2.1:+49668945 MS.gene91768:CDS
TTCTTCCTCGCCTTCCTGAA+TGG 0.401426 2.1:+49670846 MS.gene91768:CDS
CATTAATCAATTTCCATTTG+AGG 0.403628 2.1:+49673658 MS.gene91768:CDS
GAATTATAAATCTCCAGTTC+TGG 0.404024 2.1:-49670022 None:intergenic
GAGAAGGACTAGACAACCTT+TGG 0.406467 2.1:+49674078 MS.gene91768:CDS
CTTTCGTACTTGAACATTCG+TGG 0.406787 2.1:+49670493 MS.gene91768:CDS
CGGTCATGAATAGGCTCATC+AGG 0.408012 2.1:-49670872 None:intergenic
CCAACATCAAGGGCAATTTA+CGG 0.409401 2.1:+49674843 MS.gene91768:CDS
CCATTTAGTTCAGAAATGTT+TGG 0.413238 2.1:-49670770 None:intergenic
GGTTCGGAGCATGCACCCTT+TGG 0.416642 2.1:+49674265 MS.gene91768:CDS
GCCACATTGCACTTATAATA+CGG 0.416942 2.1:-49670892 None:intergenic
CTCTCAGAGGACTGCACAGT+GGG 0.420225 2.1:-49671964 None:intergenic
GGTTAGTGTTGTAGAAATGT+TGG 0.423832 2.1:+49668975 MS.gene91768:CDS
ATAATCCGACTCATGGAAAC+TGG 0.425940 2.1:+49674164 MS.gene91768:CDS
CTTATAATACGGTCATGAAT+AGG 0.427782 2.1:-49670881 None:intergenic
ATGAATTTGGTTCTGCTTTG+CGG 0.431191 2.1:+49671272 MS.gene91768:CDS
CTCTTGCTTACGTTCATTGG+AGG 0.433275 2.1:+49670570 MS.gene91768:CDS
TTGAGATGGTTGGAGCTTGA+TGG 0.434714 2.1:+49669039 MS.gene91768:CDS
CGAAAGGGATGGCAAACAAA+TGG 0.435231 2.1:-49670476 None:intergenic
CTTGGATAAAATAGTTCTCT+GGG 0.435795 2.1:-49671800 None:intergenic
AAGAGATTCCAGAATTTCAA+TGG 0.443802 2.1:-49670747 None:intergenic
GTAACTGAGCTTTGCTCCAA+TGG 0.447485 2.1:-49670520 None:intergenic
CACAAGCAATTTCGGGGAGT+GGG 0.449915 2.1:+49670051 MS.gene91768:CDS
TCTCTTCGCAAGGTTCGATC+TGG 0.454357 2.1:-49668248 None:intergenic
TGATCTCCGTTATGTAGTAC+TGG 0.455881 2.1:+49674244 MS.gene91768:CDS
AGGTTTCGGTGGAGAGTTTG+TGG 0.460242 2.1:-49668206 None:intergenic
GATGTTGGACTATGCATCTC+AGG 0.462658 2.1:-49674828 None:intergenic
TGGATATACTTGCAAGAGTA+AGG 0.462659 2.1:+49674763 MS.gene91768:CDS
TTTAGCAGAAACAATTCAAA+AGG 0.464036 2.1:+49673703 MS.gene91768:CDS
TTACCAACCTCTAGTAACTT+AGG 0.465893 2.1:-49674897 None:intergenic
ATGACAAGACAAGCCTCAAA+TGG 0.465912 2.1:-49673671 None:intergenic
ACTCTCCGACGCTTACAAAC+AGG 0.466139 2.1:+49668356 MS.gene91768:CDS
ATTCCTGTCAGATTGTTTCT+GGG 0.469381 2.1:+49674292 MS.gene91768:CDS
CCCTCTGGCATGAAAAGAAA+AGG 0.470377 2.1:-49671325 None:intergenic
TCCGTTATGTAGTACTGGTT+CGG 0.471117 2.1:+49674249 MS.gene91768:CDS
GTTCGAGAGGTGAAAGAGAA+AGG 0.471606 2.1:+49669006 MS.gene91768:CDS
TTTATCTTGCTTCAGGCACA+TGG 0.472517 2.1:+49673975 MS.gene91768:intron
TGTGGAAGAGACGGCGGTGG+AGG 0.472903 2.1:-49668188 None:intergenic
CACAGTGGGTGGTATTAAAC+TGG 0.473738 2.1:-49671950 None:intergenic
ATTGAACCAAGCAGTAAGTC+TGG 0.478752 2.1:-49671775 None:intergenic
ATCTCTTGCAGTTAAATGGT+TGG 0.478877 2.1:+49674743 MS.gene91768:intron
TCACAAGCAATTTCGGGGAG+TGG 0.481347 2.1:+49670050 MS.gene91768:CDS
TATGACATGGATACCGTTGT+TGG 0.485794 2.1:+49675141 MS.gene91768:CDS
TATAATATGGACCTGCGTAC+AGG 0.490503 2.1:+49673589 MS.gene91768:CDS
CAACCTCTAGTAACTTAGGT+TGG 0.493695 2.1:-49674893 None:intergenic
CCGTAAATTGCCCTTGATGT+TGG 0.495083 2.1:-49674843 None:intergenic
TATGATTCGATCGGTGTTTG+AGG 0.499225 2.1:+49674788 MS.gene91768:CDS
CGATATTCCTGGTCCTCTTG+GGG 0.503309 2.1:+49670718 MS.gene91768:CDS
GAAATGCTCGCCGCCGTCGA+AGG 0.504521 2.1:-49668226 None:intergenic
GAACATTCGTGGAAATCCAT+TGG 0.506538 2.1:+49670504 MS.gene91768:CDS
GAGTAGAATTCCGTGAACTT+TGG 0.507996 2.1:-49668142 None:intergenic
AGAAGTATTCGCCCACATGA+TGG 0.518087 2.1:+49671988 MS.gene91768:CDS
TTGGACCAGTTTCCATGAGT+CGG 0.519542 2.1:-49674169 None:intergenic
AGGCACATGGATCTCCTCAA+TGG 0.520514 2.1:+49673988 MS.gene91768:intron
TCCATGAGTCGGATTATGGC+TGG 0.520674 2.1:-49674158 None:intergenic
TGATGATGTTTCGGGAGAGA+AGG 0.520808 2.1:+49668954 MS.gene91768:CDS
ATCAAATTTAAGCATCTCAA+AGG 0.523069 2.1:+49669832 MS.gene91768:CDS
CTCATGCGATTCTCTTCGCA+AGG 0.523435 2.1:-49668258 None:intergenic
GAAAGGGATGGCAAACAAAT+GGG 0.527047 2.1:-49670475 None:intergenic
TGAGTTTCAAGATTATAAGT+AGG 0.529055 2.1:-49674017 None:intergenic
CCCATCCTCTCTGCTAATCC+CGG 0.529775 2.1:-49668871 None:intergenic
CCTTTCCAAACATTCCGTGT+TGG 0.533360 2.1:-49671696 None:intergenic
TACAGTAGTATCAATAGTGC+GGG 0.533708 2.1:-49674123 None:intergenic
GCCGATGCTAGATTGCCCTC+TGG 0.535309 2.1:-49671340 None:intergenic
TTAAGCATCTCAAAGGACTC+TGG 0.537705 2.1:+49669839 MS.gene91768:CDS
ACCTTTGGATCTTAAAACCC+TGG 0.538572 2.1:+49674093 MS.gene91768:CDS
GAAGTATTCGCCCACATGAT+GGG 0.539104 2.1:+49671989 MS.gene91768:CDS
TGTAGAAATGTTGGAGAGTG+AGG 0.539327 2.1:+49668984 MS.gene91768:CDS
AGTTAGACGAAACCTCTGTA+TGG 0.539543 2.1:+49670951 MS.gene91768:CDS
CACGAATGTTCAAGTACGAA+AGG 0.541790 2.1:-49670492 None:intergenic
TCCGGGATTAGCAGAGAGGA+TGG 0.541813 2.1:+49668870 MS.gene91768:CDS
GAACAACCTGCTTTATTCCA+GGG 0.542789 2.1:+49674212 MS.gene91768:CDS
TTACTGACCGGGAATCTAGC+AGG 0.542860 2.1:-49669102 None:intergenic
GTTCTGCCAGACTTACTGCT+TGG 0.544003 2.1:+49671769 MS.gene91768:CDS
GGATAATTCTAGGTACGTGA+TGG 0.545152 2.1:+49671249 MS.gene91768:intron
AGAAGAAGAAATGGTAGCAA+TGG 0.546649 2.1:+49668080 None:intergenic
CAGCTTGATCAGCATTAGAT+GGG 0.548839 2.1:-49670111 None:intergenic
TGCAAGCTCGTGTACAATCA+GGG 0.549178 2.1:-49675116 None:intergenic
GCATCCAAAATATTGGAAAG+TGG 0.551382 2.1:+49670800 MS.gene91768:CDS
GTTTGTGGAAGAGACGGCGG+TGG 0.553090 2.1:-49668191 None:intergenic
CGTAAAGGACGCCCATCATG+TGG 0.553316 2.1:-49672000 None:intergenic
GTTGGAGAGTGAGGTTCGAG+AGG 0.553772 2.1:+49668993 MS.gene91768:CDS
ATGGATACCGTTGTTGGAGA+AGG 0.554146 2.1:+49675147 MS.gene91768:CDS
ATATCTGTGTATACCCGTAA+AGG 0.555996 2.1:-49672015 None:intergenic
AACTTTGGCGAAGTCTTCGA+AGG 0.557163 2.1:-49668127 None:intergenic
TGGGACCTGTTTGTAAGCGT+CGG 0.563612 2.1:-49668361 None:intergenic
GATCTTAAAACCCTGGAACA+TGG 0.564811 2.1:+49674100 MS.gene91768:CDS
CTTACCAATTCTGAAGAACT+GGG 0.565554 2.1:-49669873 None:intergenic
ATCGGTGTTTGAGGCAGCTG+CGG 0.566183 2.1:+49674797 MS.gene91768:CDS
TGACGGTACATTGAGATGGT+TGG 0.567506 2.1:+49669029 MS.gene91768:CDS
TCCACCGAAACCTTCGACGG+CGG 0.568274 2.1:+49668216 MS.gene91768:CDS
ACCAGCCATAATCCGACTCA+TGG 0.571309 2.1:+49674157 MS.gene91768:CDS
ATACCCAGAAACAATCTGAC+AGG 0.581860 2.1:-49674295 None:intergenic
AGATGCATAGTCCAACATCA+AGG 0.583143 2.1:+49674832 MS.gene91768:CDS
ACGAATGTTCAAGTACGAAA+GGG 0.584367 2.1:-49670491 None:intergenic
GTGGAAGAGACGGCGGTGGA+GGG 0.585364 2.1:-49668187 None:intergenic
GGTTGATGAGGAGTTGAAGA+AGG 0.587491 2.1:+49673610 MS.gene91768:CDS
AACAACCTGCTTTATTCCAG+GGG 0.587507 2.1:+49674213 MS.gene91768:CDS
TTTCCAACCTAAGTTACTAG+AGG 0.588700 2.1:+49674890 MS.gene91768:CDS
GAGGAGTTGAAGAAGGCCAC+GGG 0.591379 2.1:+49673617 MS.gene91768:CDS
TTGCGCAGAGATATATGGAA+AGG 0.594711 2.1:+49670084 MS.gene91768:CDS
GCCGCCGTCGAAGGTTTCGG+TGG 0.597756 2.1:-49668217 None:intergenic
GCCAGAGGGCAATCTAGCAT+CGG 0.598031 2.1:+49671339 MS.gene91768:CDS
TGAGGAGTTGAAGAAGGCCA+CGG 0.599213 2.1:+49673616 MS.gene91768:CDS
GGAGAAGGAGGTATTGCCAA+AGG 0.601959 2.1:+49675162 MS.gene91768:CDS
AATACCTCCTTCTCCAACAA+CGG 0.603511 2.1:-49675154 None:intergenic
TTGCTGATCTGTTATTCCCG+TGG 0.606174 2.1:-49673633 None:intergenic
CACTTACTGATTGCAAACTC+AGG 0.606354 2.1:-49672066 None:intergenic
CCGGGATTAGCAGAGAGGAT+GGG 0.606835 2.1:+49668871 MS.gene91768:CDS
AACTTTCACAAGCAATTTCG+GGG 0.606957 2.1:+49670045 MS.gene91768:CDS
TTTCTATTCTATGGCCAACA+CGG 0.607111 2.1:+49671682 MS.gene91768:CDS
ATAGTGCGGGCCATGTTCCA+GGG 0.608612 2.1:-49674110 None:intergenic
CTGACAGGAATATCTCCAAA+GGG 0.611128 2.1:-49674280 None:intergenic
TCTGACAGGAATATCTCCAA+AGG 0.611646 2.1:-49674281 None:intergenic
GGAGTCCATTCAGGAAGGCG+AGG 0.613302 2.1:-49670851 None:intergenic
TGGAGAGTTTGTGGAAGAGA+CGG 0.613450 2.1:-49668197 None:intergenic
GATAGTGAAACTGAGGTGAA+TGG 0.613747 2.1:+49668922 MS.gene91768:CDS
AAGTCCAAGCATGATCAACA+AGG 0.621301 2.1:-49668329 None:intergenic
AGTAAGGACTATGATTCGAT+CGG 0.623174 2.1:+49674779 MS.gene91768:CDS
GCTCTCAGAGGACTGCACAG+TGG 0.626388 2.1:-49671965 None:intergenic
CATTCACAATCTGATCAACA+AGG 0.628020 2.1:+49670180 MS.gene91768:CDS
GATGGGTTCTTTGATGTGTG+TGG 0.629001 2.1:+49668888 MS.gene91768:CDS
GATACCGTTGTTGGAGAAGG+AGG 0.629194 2.1:+49675150 MS.gene91768:CDS
GATGCATAGTCCAACATCAA+GGG 0.630418 2.1:+49674833 MS.gene91768:CDS
TATTATGTACGCAAAAGAGA+AGG 0.632002 2.1:+49674062 MS.gene91768:CDS
ATGATCAACAAGGAAGACAT+TGG 0.633048 2.1:-49668319 None:intergenic
CTCCTCATCAACCTGTACGC+AGG 0.634177 2.1:-49673600 None:intergenic
TCCGAACCAGTACTACATAA+CGG 0.635574 2.1:-49674250 None:intergenic
CCAACACGGAATGTTTGGAA+AGG 0.635916 2.1:+49671696 MS.gene91768:CDS
TAAGTAGGCTGCAGCCATTG+AGG 0.635991 2.1:-49674002 None:intergenic
ATGTTCAAGTACGAAAGGGA+TGG 0.635997 2.1:-49670487 None:intergenic
GCATGTTCTCAGAATTATCG+TGG 0.636330 2.1:+49674562 MS.gene91768:intron
TCAGAGGACTGCACAGTGGG+TGG 0.639769 2.1:-49671961 None:intergenic
AAGGTGACGGTACATTGAGA+TGG 0.641533 2.1:+49669025 MS.gene91768:CDS
GTCAAGTACTCTTCCACGTG+AGG 0.644264 2.1:-49672039 None:intergenic
AGTGGGTGGTATTAAACTGG+TGG 0.645039 2.1:-49671947 None:intergenic
GACCTGCGTACAGGTTGATG+AGG 0.645215 2.1:+49673598 MS.gene91768:CDS
AGATTAGATACACTCAGCAG+TGG 0.646587 2.1:-49670139 None:intergenic
TCTCAGCAGCTCTAACAAGT+CGG 0.647266 2.1:-49670542 None:intergenic
TTACAGTAGTATCAATAGTG+CGG 0.648290 2.1:-49674124 None:intergenic
GCAATCTAGCATCGGCTGTG+AGG 0.649411 2.1:+49671347 MS.gene91768:CDS
GAATTTGAGGAAGAACATCA+AGG 0.650482 2.1:+49674622 MS.gene91768:CDS
ACGAGCTTGCAAATATGACA+TGG 0.651494 2.1:+49675128 MS.gene91768:CDS
CATCAGGAGTCCATTCAGGA+AGG 0.654679 2.1:-49670856 None:intergenic
CTCTCCACCGAAACCTTCGA+CGG 0.658578 2.1:+49668213 MS.gene91768:CDS
GATATTCCTGGTCCTCTTGG+GGG 0.661353 2.1:+49670719 MS.gene91768:CDS
GAGGTGAAAGAGAAAGGTGA+CGG 0.664017 2.1:+49669012 MS.gene91768:CDS
TGATTTGGATAGTGAAACTG+AGG 0.668818 2.1:+49668915 MS.gene91768:CDS
GGATTCCGGTAAACCCGAAG+CGG 0.670174 2.1:-49668166 None:intergenic
TGATGAGCCAAACTTTAGAG+TGG 0.680214 2.1:+49671300 MS.gene91768:CDS
ATACACAGATATACCTCACG+TGG 0.685643 2.1:+49672026 MS.gene91768:CDS
GTAAAGGACGCCCATCATGT+GGG 0.688444 2.1:-49671999 None:intergenic
AAGTTCCGGGATTAGCAGAG+AGG 0.689466 2.1:+49668866 MS.gene91768:CDS
GCGAATACTTCTGCTCTCAG+AGG 0.689727 2.1:-49671977 None:intergenic
ATGAATGTCATACCATACAG+AGG 0.704504 2.1:-49670963 None:intergenic
TTGGATAAAATAGTTCTCTG+GGG 0.706460 2.1:-49671799 None:intergenic
AGAGTTTGTGGAAGAGACGG+CGG 0.716886 2.1:-49668194 None:intergenic
TAGAATCCGAAGTCAAAACG+AGG 0.732532 2.1:-49668284 None:intergenic
ACCGTATTATAAGTGCAATG+TGG 0.733685 2.1:+49670891 MS.gene91768:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.1 gene 49668090 49675341 49668090 ID=MS.gene91768
chr2.1 mRNA 49668090 49675341 49668090 ID=MS.gene91768.t1;Parent=MS.gene91768
chr2.1 exon 49668090 49668377 49668090 ID=MS.gene91768.t1.exon1;Parent=MS.gene91768.t1
chr2.1 CDS 49668090 49668377 49668090 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49668859 49669116 49668859 ID=MS.gene91768.t1.exon2;Parent=MS.gene91768.t1
chr2.1 CDS 49668859 49669116 49668859 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49669766 49669890 49669766 ID=MS.gene91768.t1.exon3;Parent=MS.gene91768.t1
chr2.1 CDS 49669766 49669890 49669766 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49669985 49670201 49669985 ID=MS.gene91768.t1.exon4;Parent=MS.gene91768.t1
chr2.1 CDS 49669985 49670201 49669985 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49670466 49670591 49670466 ID=MS.gene91768.t1.exon5;Parent=MS.gene91768.t1
chr2.1 CDS 49670466 49670591 49670466 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49670716 49670972 49670716 ID=MS.gene91768.t1.exon6;Parent=MS.gene91768.t1
chr2.1 CDS 49670716 49670972 49670716 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49671261 49671368 49671261 ID=MS.gene91768.t1.exon7;Parent=MS.gene91768.t1
chr2.1 CDS 49671261 49671368 49671261 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49671680 49671821 49671680 ID=MS.gene91768.t1.exon8;Parent=MS.gene91768.t1
chr2.1 CDS 49671680 49671821 49671680 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49671916 49672081 49671916 ID=MS.gene91768.t1.exon9;Parent=MS.gene91768.t1
chr2.1 CDS 49671916 49672081 49671916 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49673567 49673724 49673567 ID=MS.gene91768.t1.exon10;Parent=MS.gene91768.t1
chr2.1 CDS 49673567 49673724 49673567 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49673990 49674313 49673990 ID=MS.gene91768.t1.exon11;Parent=MS.gene91768.t1
chr2.1 CDS 49673990 49674313 49673990 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49674400 49674474 49674400 ID=MS.gene91768.t1.exon12;Parent=MS.gene91768.t1
chr2.1 CDS 49674400 49674474 49674400 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49674574 49674643 49674574 ID=MS.gene91768.t1.exon13;Parent=MS.gene91768.t1
chr2.1 CDS 49674574 49674643 49674574 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49674754 49674911 49674754 ID=MS.gene91768.t1.exon14;Parent=MS.gene91768.t1
chr2.1 CDS 49674754 49674911 49674754 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49675105 49675239 49675105 ID=MS.gene91768.t1.exon15;Parent=MS.gene91768.t1
chr2.1 CDS 49675105 49675239 49675105 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
chr2.1 exon 49675315 49675341 49675315 ID=MS.gene91768.t1.exon16;Parent=MS.gene91768.t1
chr2.1 CDS 49675315 49675341 49675315 ID=cds.MS.gene91768.t1;Parent=MS.gene91768.t1
Gene Sequence

>MS.gene91768

ATGGTAGCAATGGCAACAACAACAAAGAGAATCGAAACCTTCGAAGACTTCGCCAAAGTTCACGGAATTCTACTCGCCGCTTCGGGTTTACCGGAATCCCTCCACCGCCGTCTCTTCCACAAACTCTCCACCGAAACCTTCGACGGCGGCGAGCATTTCCAGATCGAACCTTGCGAAGAGAATCGCATGAGACGCCTCGTTTTGACTTCGGATTCTATGGCTAAATACTCCAATGTCTTCCTTGTTGATCATGCTTGGACTTTCAGACTCTCCGACGCTTACAAACAGGTCCCAACCTTCTTCACATTCTTCACGCAACACTAGTTTTAAGTAACTTCGATGAAAATAGCTTATGACATGTTTATAAGTCCTTTTTAGCTTATTTTCAAGATAGTTTATGAAAACTATTTATAGTTTATGCGAAAACAAATTAATTTTATTTTATCTTTTGTTATAGAAATATGTTATACATAAGCTCTTATTGCGATAAGCACTTATGTTAATAGCTACAAATTAAGTTGTTTATCCAAATCGAGCCTAAATTGATATTAAGTGGTTAAATATAAGGACATGATTGATGGTAAGTTGTTAAAATGTTTGGGACTAACTTGATAGTAAGTGGTTAAATACAGGGACTAACTTGAGAGCACCCTACAAAGTTAGGGACTATTTTGGTGGTTCACTCACTAATTTTCTATAACTAGGATTTCATTTTTACGTTAACTTTATCATTTTTGTTTTGTTTTTTTTGCTTTTGTGGTGATTTTAGTTGCGTGAAGTTCCGGGATTAGCAGAGAGGATGGGTTCTTTGATGTGTGTGGATATTGATTTGGATAGTGAAACTGAGGTGAATGGTGTTGATGATGATGATGTTTCGGGAGAGAAGGTTAGTGTTGTAGAAATGTTGGAGAGTGAGGTTCGAGAGGTGAAAGAGAAAGGTGACGGTACATTGAGATGGTTGGAGCTTGATGGTCTTGATATCGATGATGTTATGTTTCTCTCATTGGACTTGCCTGCTAGATTCCCGGTCAGTAACTCTTAATCCTGATTTTTTTATTTTGTTCATATTTTATTTTATAGTTATAATTTCTATAGGTTTTATTGTTTTGAAAGTGTGACTTTGTTCATTCTGCAAATAGAAATCATGCTCATCAAACTTAGGAATGTTATATTATGATATGAATTGAATTTGAGTGGCAAGATGGCAAACATTATTACTTCCTTCATCCGTTATTATAAGACTCACTTTTGACTTTTGTTAAAAAAATTAAGACTTTTGATATTTCCTTGTTCTTTAATATTGGACTCTTTTCAAAATTTTACATTCAATAATAATTACTTTACAAAAATTTCCTTATTTAATTTACAATTGTGAAACGTAAATTTGTTTTTTCACTCTTACAACATCAATAATTGTGAGGATATTTATAGGAAGTAACACAAATTTGACAGGTTTATTGTTATGGCTATTTTTCTTAATACCGAGTAAATTTTGTAAGCGAGTCTCATAATAAGAGACGATGACAGTATTATTTAATTATCGTCGGAGTATTCAATTTTCCTGTCATCTCAGATCCCGTTAGTCTAGATGCTGCAAGCCAATTTTAGTTTCACGTTTCTTGTTATAATTATTTTAATTTGAATTCTGATGAACTATCCTCCCCTTTCCCTTTCAGAATTTAGTGGCACTTAGCCTATATGGAAACAAGCTTACAAGACCTGAATTGATTATTCAAGAAGTTATCAAATTTAAGCATCTCAAAGGACTCTGGCTGAACAATAACCCAGTTCTTCAGAATTGGTAAGTTTTCTCATATTTTAAAAATTTCTTAAACCACATATTCCTTTTTGGTAAGTCCTTGTTGTTAAGGAGAGCTAAATTACTTTACTTCTAGTGATGGTGATCTTGCAGGTATTATTCTTAAGGAGTTACCAGAACTGGAGATTTATAATTCAACTTTCACAAGCAATTTCGGGGAGTGGGCTTTGGGCTTTTGCGCAGAGATATATGGAAAGGATAACCCATCTAATGCTGATCAAGCTGACAGTCCACTGCTGAGTGTATCTAATCTTGATCTTTCAAATAGAAACATTCACAATCTGATCAACAAGGTTGAACCTTTTGTTATGCTTATTTTCTATCTTATTGGCTTATGTTTCAAAAGTATAGATAGACTTAAGCATATTATTGTACTGAGTTCAAAAATCTGATAATTATGAATCTCTCCTTGAGTCTATGCTCAAGAGATACAAAATGGTAAACAAAGCATAATCACTAAAAAGATTTTAAACACATGGCATTGCCAATTCATTTTTTCCAATCCCATATATGTATGTTTTGATCGAATTATTTTGACGATTCCCTCATTTGGTTAGGACTTCTCACCCATTTGTTTGCCATCCCTTTCGTACTTGAACATTCGTGGAAATCCATTGGAGCAAAGCTCAGTTACCGACTTGTTAGAGCTGCTGAGACGATTCTCTTGCTTACGTTCATTGGAGGTACTTTGATTTATGTTATGACAATTTTTCAAGCCCCAAATTGCCTTTGTTATTTATTTAATTTTGTATGTCCATTCTCTGGTAACCAGTGTTTTGGCCTTTGGGAATTTGAGCAAATTTTTAGGTCGATATTCCTGGTCCTCTTGGGGGAAGTGCCATTGAAATTCTGGAATCTCTTCCAAACATTTCTGAACTAAATGGTATTTGTGCATCCAAAATATTGGAAAGTGGAAAGCATGTTATTGATTCAGTGCTTCTTCCTCGCCTTCCTGAATGGACTCCTGATGAGCCTATTCATGACCGTATTATAAGTGCAATGTGGCAATATCTAATGACATATAGACTTGCTGATGAAGAAAAGTTAGACGAAACCTCTGTATGGTATGACATTCATTTTACTTGTTTTACTTTCTTGGTTCTAATGTAGCACTAGGTTTCTATCAATCAGTAAATGCTTTTAAGTTTTGTGTTTTTGCACTTCTTGTTGAAATACACAATTTCATCAAGGTTGTCTGATTTGTAAATTATTGTTGTGACCCTACTCGAATCAATGGGTCAATGTTTAACCATTCAAATTGTTGGGTCAGTACATTATATGAAACATATGATCAATTTGGAATTTCCCAACAATAAAGAAAACTAACATCCCATTGTTTTGGATAATTCTAGGTACGTGATGGATGAATTTGGTTCTGCTTTGCGGCACAGTGATGAGCCAAACTTTAGAGTGGCACCTTTTCTTTTCATGCCAGAGGGCAATCTAGCATCGGCTGTGAGGTTTTCTTCTATATTTTACTTTTATATTTTGCTTTTCTACTATATTTTACTTTCTGCTTTACTATATGATTGAGCTTGCATGATTTGTAAAAAAATATTATATAAAAAAATTCGATTATAGGTTTTGCTCTGTAATTCTTGCTCATTAATATATAATAACTTAATTACCTGTGTAGCTCATATTAATGACTTCTAACCGCGAAACTTCTCCTCCATCCTTTTTATGAATTACATGTATTTTATGGCATGCCCTCTGGATTTGTGATAATTCTAAATTAGTCTCTGTAAGTAATAACTCCCACTTTTCACAGCTTTTCTATTCTATGGCCAACACGGAATGTTTGGAAAGGCGATGAATGCACTCGTGACTTTCTTCTTGGTATTGGAGAAGATAAACAGCGTTCTGCCAGACTTACTGCTTGGTTCAATACCCCAGAGAACTATTTTATCCAAGTAATTCTTCTATTACTAAGGCAGATTATGATTCATATCTGTACCACACGAAAGTTTTAGGGAAGTTTGAGGCTAATTATTTTATTATTTTCAGGAGTATGAGAAGTACAATCAGAGATTGCAATCCACCAGTTTAATACCACCCACTGTGCAGTCCTCTGAGAGCAGAAGTATTCGCCCACATGATGGGCGTCCTTTACGGGTATACACAGATATACCTCACGTGGAAGAGTACTTGACACATCCTGAGTTTGCAATCAGTAAGTGGACTCTATGCATCTGATAGACACTGGATTAAATTATAGAGATTAACAGAATGAAAATAATGAAATTTTATCACATATCCTCCTCCAACTTTTGTTCCTTTTTGTTAGGTTGCTTTGAAAAAGGCACAAGCAGGGAATTATTCGTCCCTGAGTTTATTGATAACCGAAAGGTTTATTGATAACAGAAAGAAAAGGGTTACTTTCCTCACCGGAATAAGCCAATTTCTTAATAAAGTTCAGAATGCCTAATCTAGGGGCCGGAGAAGCTATTTATAGATTCCCCGGAATAAGCCAGTCAATTTGTTAATAAAAGTTCAGAATGCCTAATCTAGGGTTACAGCCTATGTGACTGGCAGTTGCCAATTAAGACGGTTACAGCCTGTCAAGGCAGTTACAAGGGAAATGCTCTAGTGAGTCACAGCTTATGGTGATCATTCTGAATTCTGGTCACTTACTACCTATATTTGGAGTCAATCCTTTGATATGTCTAACACTTTTCTTGATCTTAAAATCTGTTTTCTTGAACATCACCTCACCTCTCAGAAGCTTTCCCAGGTTTCCTTAGGATTGTTAACCTCCCACTACTTGTACCAATGTTGGAATAGTAGTGCTAGCAACACTCTCTTTTGAGCACTCTCTCTAATACTCACTCTCTTATTGGTTTAAATCATTGTGGGTCTATACTTTGGAAATATAATGGCGAGACATACATTGATTCCATCCAATAAAAGAGTGAGTGCTAGAGAGTGTTCAAAAGTGAGTGTTGCCAGCATTCCTCATGTCGGAATGTTCACCCAGTGCTTCCACGCCCATCATTTTCTGTGTCGAAGCGCTCCTCTGCAAATCTTCTTGAATTTACTTCTTGATCAGTCTACACAAAGTTCCCCATTTCTTGGGAGCTTCAGTACTAGTTGATAGGTACTGGTTCAATATATGGAGGCATACAAATGGAAAATGAGGAAGTTTTATAATAATTCCTCCTAACTTAACTCTGGATGAATGGTTATCCAGGTCAAGATGAGCAGGATGACATATAATGATGTCTTCCCCAGGTATTTGAGCTGGTCTCTCCTAAGTCCCACTCAAAACTAATTAACTTCCTCTAGCTTCTTTGCCTCTATTAAACAGCCTAACTAATTAACCACTCTAACAACTTTCCTAACAGAGACTGTGCTTGGCTAGCAGGGTTCCTTGACCTAACTGATGTAAGCTTTCCTAATTGGGTCTGCTCCTAACAACATGCTTGAACTCTTTTATCAACTCATAATATACAGTTAAACAATCATGTAACTTGAAAAGTTAAGAGCCTAAATTGAATTTTCCTTGTTTCATCTGAACCTTTTCTCATTCTGAGAAGCATTGACATCTTATGTTTTTCATAAAATTCCCATGGATATTTGATTCAATGATTACCTCCACGTTTTTAAAGTTTGCTGGATTCTGACCTTCTCCCTCTCTTTTGAATGTGTTTGGCTACAGTAAAGGACCCAAAAGATGCAGATATAATATGGACCTGCGTACAGGTTGATGAGGAGTTGAAGAAGGCCACGGGAATAACAGATCAGCAATACATTAATCAATTTCCATTTGAGGCTTGTCTTGTCATGAAGCATCATTTAGCAGAAACAATTCAAAAGGTGATATTATAGTTAATCAACATTGTATGTTGATGCTGATGAGATTATCCCAAATGTCTGACCTGTTTTCATTATGCATTTCTAGTGAAATTCCATCATACATTCAGAGAAATATGTATTATATTATATATTATATATTCATCAGAGCATGCTACATTTAATTATATTTTCAAGTCACTTACAAATGAATTGAAGTGTTGGTTTCTCTTTTGAGGCTTTTTAAATAATGAATCATTTTGGGGTTGGGTGTTTTATCTTGCTTCAGGCACATGGATCTCCTCAATGGCTGCAGCCTACTTATAATCTTGAAACTCATCTGTCTCAACTTATTGGTGACTATTATGTACGCAAAAGAGAAGGACTAGACAACCTTTGGATCTTAAAACCCTGGAACATGGCCCGCACTATTGATACTACTGTAACTGATAACTTACCAGCCATAATCCGACTCATGGAAACTGGTCCAAAAATATGCCAGAAGTATATTGAACAACCTGCTTTATTCCAGGGGAAAAAGTTTGATCTCCGTTATGTAGTACTGGTTCGGAGCATGCACCCTTTGGAGATATTCCTGTCAGATTGTTTCTGGGTATACTGATTCTTCTTTCTTTATTTTATTCTTATGCTCTTAAAAGGTTAAACTCTAATGGCAATGAATTTTATGTTTTATTTCAGGTTAGGATAGCTAACAACCAATATTCTTTGGCCAAAAGTAGTCTTTTTGAGTATGAAACTCATTTCACTGTTATGGTATGTCTTGAATTCCATATTGTTTAGCAACAAGATGTTTGGTTGTGCTTCCCTTTTTCTTATCAAGTTTTAACATATTCAATCAATGCATGTTCTCAGAATTATCGTGGAAGAATAAATCACAAGAATATTAAGGATTTTGTGAGGGAATTTGAGGAAGAACATCAAGGTAATGAGGGCTGCCACATTCTATAGATGAATTTTTAGAGGCTTCCAGAGCATATTATATATTTTGAATGAATTTTTTTATACAAATTCATGTCTCTGTATCTCTTGCAGTTAAATGGTTGGATATACTTGCAAGAGTAAGGACTATGATTCGATCGGTGTTTGAGGCAGCTGCGGTTGCACATCCTGAGATGCATAGTCCAACATCAAGGGCAATTTACGGTGTAGATGTCATGTTAGACAGCTCTTTCCAACCTAAGTTACTAGAGGTTGGTAACCGAAACAGTGGTTCTCATTTGATGCTCCTTTCAATACCAAAATTTTTTATATTGACCTGAACATCTTGATTCAAGAATACAAATTTGTAATGTACTATTTGTTTAATGCTGAATTATCTGATTAGGATGCATTTCACCATACGAATTATCGCATTACATGTATGACTTGTATTTTATATTGCAGGTGACTTACTGCCCTGATTGTACACGAGCTTGCAAATATGACATGGATACCGTTGTTGGAGAAGGAGGTATTGCCAAAGGTTGTGATTTCTTCAACAATGTTTTCAAGTGTCTCTTTTTGAATGAAATTTCTCAGGTTAGTCAATTGTAATCTGTTCATCACAGAGATTTGACATCCTTAATTAAAGCCTTCTTCGACAACGGACTACAGGTTCTTTTAGCAAGGCCTGGAAATTAG
Protein sequence

>MS.gene91768.t1

MVAMATTTKRIETFEDFAKVHGILLAASGLPESLHRRLFHKLSTETFDGGEHFQIEPCEENRMRRLVLTSDSMAKYSNVFLVDHAWTFRLSDAYKQLREVPGLAERMGSLMCVDIDLDSETEVNGVDDDDVSGEKVSVVEMLESEVREVKEKGDGTLRWLELDGLDIDDVMFLSLDLPARFPNLVALSLYGNKLTRPELIIQEVIKFKHLKGLWLNNNPVLQNCDGDLAGIILKELPELEIYNSTFTSNFGEWALGFCAEIYGKDNPSNADQADSPLLSVSNLDLSNRNIHNLINKDFSPICLPSLSYLNIRGNPLEQSSVTDLLELLRRFSCLRSLEVDIPGPLGGSAIEILESLPNISELNGICASKILESGKHVIDSVLLPRLPEWTPDEPIHDRIISAMWQYLMTYRLADEEKLDETSVWYVMDEFGSALRHSDEPNFRVAPFLFMPEGNLASAVSFSILWPTRNVWKGDECTRDFLLGIGEDKQRSARLTAWFNTPENYFIQEYEKYNQRLQSTSLIPPTVQSSESRSIRPHDGRPLRVYTDIPHVEEYLTHPEFAIIKDPKDADIIWTCVQVDEELKKATGITDQQYINQFPFEACLVMKHHLAETIQKAHGSPQWLQPTYNLETHLSQLIGDYYVRKREGLDNLWILKPWNMARTIDTTVTDNLPAIIRLMETGPKICQKYIEQPALFQGKKFDLRYVVLVRSMHPLEIFLSDCFWVRIANNQYSLAKSSLFEYETHFTVMNYRGRINHKNIKDFVREFEEEHQVKWLDILARVRTMIRSVFEAAAVAHPEMHSPTSRAIYGVDVMLDSSFQPKLLEVTYCPDCTRACKYDMDTVVGEGGIAKGCDFFNNVFKCLFLNEISQVLLARPGN