Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91794.t1 | XP_013443805.1 | 94.6 | 168 | 9 | 0 | 1 | 168 | 1 | 168 | 6.00E-79 | 303.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91794.t1 | I3SHZ2 | 94.6 | 168 | 9 | 0 | 1 | 168 | 1 | 168 | 4.3e-79 | 303.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049698 | MS.gene91794 | 0.800523 | 1.39E-48 | -1.69E-46 |
| MS.gene055371 | MS.gene91794 | 0.949682 | 7.76E-108 | -1.69E-46 |
| MS.gene058909 | MS.gene91794 | 0.824293 | 8.73E-54 | -1.69E-46 |
| MS.gene058912 | MS.gene91794 | 0.822294 | 2.56E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91794.t1 | MTR_8g006765 | 94.643 | 168 | 9 | 0 | 1 | 168 | 1 | 168 | 1.26e-114 | 322 |
| MS.gene91794.t1 | MTR_4g094628 | 59.627 | 161 | 62 | 3 | 1 | 160 | 1 | 159 | 7.58e-62 | 191 |
| MS.gene91794.t1 | MTR_7g407110 | 50.704 | 71 | 33 | 2 | 1 | 71 | 148 | 216 | 1.35e-14 | 70.1 |
| MS.gene91794.t1 | MTR_8g021220 | 60.870 | 46 | 18 | 0 | 82 | 127 | 33 | 78 | 2.62e-13 | 62.4 |
| MS.gene91794.t1 | MTR_8g009870 | 28.333 | 120 | 83 | 2 | 32 | 148 | 33 | 152 | 5.23e-12 | 60.8 |
| MS.gene91794.t1 | MTR_8g009870 | 28.333 | 120 | 83 | 2 | 32 | 148 | 29 | 148 | 5.93e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91794.t1 | AT4G37445 | 52.201 | 159 | 75 | 1 | 6 | 163 | 3 | 161 | 1.64e-54 | 170 |
| MS.gene91794.t1 | AT4G37445 | 53.472 | 144 | 67 | 0 | 20 | 163 | 2 | 145 | 1.16e-51 | 162 |
Find 32 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAAAGTGAAAATTAAAT+AGG | 0.234371 | 8.3:-81327673 | None:intergenic |
| CATTGATTTCATGATCAATA+AGG | 0.282087 | 8.3:+81327444 | MS.gene91794:CDS |
| TTTCTTCAATGACATACTTT+TGG | 0.319686 | 8.3:-81327644 | None:intergenic |
| ACCATCACACAAGGAAATTA+AGG | 0.323749 | 8.3:+81327366 | MS.gene91794:CDS |
| GATGATTCTATGATGATAAC+TGG | 0.356747 | 8.3:+81327484 | MS.gene91794:CDS |
| CAGACATTAGATCTTGTATC+AGG | 0.382635 | 8.3:+81326781 | MS.gene91794:CDS |
| CTGATACAAGATCTAATGTC+TGG | 0.398337 | 8.3:-81326780 | None:intergenic |
| ATGAGTTACATGGGAGGATC+AGG | 0.435033 | 8.3:+81326602 | MS.gene91794:CDS |
| TATGATGATAACTGGAATAG+TGG | 0.452300 | 8.3:+81327492 | MS.gene91794:CDS |
| GCTATGGTAGCTAAGAGAAC+AGG | 0.456395 | 8.3:+81327526 | MS.gene91794:CDS |
| CTCTTAGCTACCATAGCTGC+TGG | 0.471596 | 8.3:-81327520 | None:intergenic |
| ATGTTTCTTCTTATCTTCCT+CGG | 0.474190 | 8.3:-81327416 | None:intergenic |
| ACTATTAACATGGCCTTGCC+TGG | 0.488789 | 8.3:+81327328 | MS.gene91794:CDS |
| TCCTTAATTTCCTTGTGTGA+TGG | 0.508644 | 8.3:-81327367 | None:intergenic |
| ATCATAATGCTTACCAGGCA+AGG | 0.514482 | 8.3:-81327341 | None:intergenic |
| GAGCTAAAATAGTAGCTGTT+GGG | 0.514762 | 8.3:-81327603 | None:intergenic |
| GTTGGGACAAAGATAACATC+AGG | 0.516476 | 8.3:-81327586 | None:intergenic |
| ATCAAGAATGGCAGTGTGGA+AGG | 0.518421 | 8.3:-81326851 | None:intergenic |
| AGAGCTAAAATAGTAGCTGT+TGG | 0.518631 | 8.3:-81327604 | None:intergenic |
| AGTGGCACCTCCAGCAGCTA+TGG | 0.520301 | 8.3:+81327510 | MS.gene91794:CDS |
| TTACTTGAAGATATCAAGAA+TGG | 0.537162 | 8.3:-81326863 | None:intergenic |
| GGGGATGATCATGAGTTACA+TGG | 0.552167 | 8.3:+81326592 | MS.gene91794:CDS |
| GGTACATCATAATGCTTACC+AGG | 0.555755 | 8.3:-81327346 | None:intergenic |
| GACAAAGATAACATCAGGAA+TGG | 0.591291 | 8.3:-81327581 | None:intergenic |
| TGATACAAGATCTAATGTCT+GGG | 0.602501 | 8.3:-81326779 | None:intergenic |
| TTAGCTACCATAGCTGCTGG+AGG | 0.629958 | 8.3:-81327517 | None:intergenic |
| GCTTTCATAACACTTAGTTG+AGG | 0.635041 | 8.3:-81327559 | None:intergenic |
| GGGATGATCATGAGTTACAT+GGG | 0.647259 | 8.3:+81326593 | MS.gene91794:CDS |
| AAACTGGAATAAAACATCCG+AGG | 0.684088 | 8.3:+81327399 | MS.gene91794:CDS |
| TTATGATGTACCATCACACA+AGG | 0.699802 | 8.3:+81327357 | MS.gene91794:CDS |
| AGATATCAAGAATGGCAGTG+TGG | 0.711163 | 8.3:-81326855 | None:intergenic |
| ATGATCATGAGTTACATGGG+AGG | 0.727517 | 8.3:+81326596 | MS.gene91794:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTTGTAAAAAAAAAAAAAA+AGG | + | chr8.3:81326919-81326938 | MS.gene91794:intron | 10.0% |
| !! | ATAAAAAAAAAAAAAACTAG+TGG | - | chr8.3:81326659-81326678 | None:intergenic | 10.0% |
| !!! | TTTTTTTTTTTTTTATTGCT+AGG | + | chr8.3:81326663-81326682 | MS.gene91794:intron | 10.0% |
| !! | AAAAAAAAAAAAAAGGAAAG+TGG | + | chr8.3:81326926-81326945 | MS.gene91794:intron | 15.0% |
| !! | AAAAAAAAAAAAAGGAAAGT+GGG | + | chr8.3:81326927-81326946 | MS.gene91794:intron | 15.0% |
| !! | AAAATTTAACATATTTCATC+AGG | + | chr8.3:81326742-81326761 | MS.gene91794:intron | 15.0% |
| !!! | TCAAATAATCATCTAAAATA+TGG | + | chr8.3:81327042-81327061 | MS.gene91794:intron | 15.0% |
| !! | AAAAAAAAAAAAGGAAAGTG+GGG | + | chr8.3:81326928-81326947 | MS.gene91794:intron | 20.0% |
| !! | AAAAAAACTAGTGGAATTAA+AGG | - | chr8.3:81326650-81326669 | None:intergenic | 20.0% |
| !! | CAAACTATATACTAGTATAA+TGG | + | chr8.3:81326983-81327002 | MS.gene91794:intron | 20.0% |
| !! | TTCAACATTTGATATCAATT+TGG | + | chr8.3:81327265-81327284 | MS.gene91794:intron | 20.0% |
| !! | TTGATATCAAATGTTGAATA+TGG | - | chr8.3:81327263-81327282 | None:intergenic | 20.0% |
| ! | ACCTTAGTTGCATAAATTTA+AGG | - | chr8.3:81327203-81327222 | None:intergenic | 25.0% |
| ! | ATATGGTCATGGTAAAATTA+AGG | + | chr8.3:81327059-81327078 | MS.gene91794:intron | 25.0% |
| ! | CCTTAGTTGCATAAATTTAA+GGG | - | chr8.3:81327202-81327221 | None:intergenic | 25.0% |
| ! | TTACTTGAAGATATCAAGAA+TGG | - | chr8.3:81326866-81326885 | None:intergenic | 25.0% |
| !! | AATCATCTAAAATATGGTCA+TGG | + | chr8.3:81327048-81327067 | MS.gene91794:intron | 25.0% |
| !! | GATTATCATTTTGTCAAAAG+TGG | - | chr8.3:81327151-81327170 | None:intergenic | 25.0% |
| !! | TTTCTTCAATGACATACTTT+TGG | - | chr8.3:81327647-81327666 | None:intergenic | 25.0% |
| !! | TTTTACTAGCACTATTAACA+TGG | + | chr8.3:81327318-81327337 | MS.gene91794:intron | 25.0% |
| !!! | CATTGATTTCATGATCAATA+AGG | + | chr8.3:81327444-81327463 | MS.gene91794:CDS | 25.0% |
| ATGTTTCTTCTTATCTTCCT+CGG | - | chr8.3:81327419-81327438 | None:intergenic | 30.0% | |
| CCCTTAAATTTATGCAACTA+AGG | + | chr8.3:81327199-81327218 | MS.gene91794:intron | 30.0% | |
| GATGATTCTATGATGATAAC+TGG | + | chr8.3:81327484-81327503 | MS.gene91794:CDS | 30.0% | |
| TAACATATTTCATCAGGTGA+GGG | + | chr8.3:81326748-81326767 | MS.gene91794:intron | 30.0% | |
| TATGATGATAACTGGAATAG+TGG | + | chr8.3:81327492-81327511 | MS.gene91794:CDS | 30.0% | |
| TGATACAAGATCTAATGTCT+GGG | - | chr8.3:81326782-81326801 | None:intergenic | 30.0% | |
| TTAACATATTTCATCAGGTG+AGG | + | chr8.3:81326747-81326766 | MS.gene91794:intron | 30.0% | |
| ! | TTAAGGAAGTTTTCGAAAAC+TGG | + | chr8.3:81327383-81327402 | MS.gene91794:CDS | 30.0% |
| AAACTGGAATAAAACATCCG+AGG | + | chr8.3:81327399-81327418 | MS.gene91794:CDS | 35.0% | |
| ACCATCACACAAGGAAATTA+AGG | + | chr8.3:81327366-81327385 | MS.gene91794:CDS | 35.0% | |
| AGAGCTAAAATAGTAGCTGT+TGG | - | chr8.3:81327607-81327626 | None:intergenic | 35.0% | |
| CAGACATTAGATCTTGTATC+AGG | + | chr8.3:81326781-81326800 | MS.gene91794:CDS | 35.0% | |
| CTGATACAAGATCTAATGTC+TGG | - | chr8.3:81326783-81326802 | None:intergenic | 35.0% | |
| GACAAAGATAACATCAGGAA+TGG | - | chr8.3:81327584-81327603 | None:intergenic | 35.0% | |
| GAGCTAAAATAGTAGCTGTT+GGG | - | chr8.3:81327606-81327625 | None:intergenic | 35.0% | |
| GCTTTCATAACACTTAGTTG+AGG | - | chr8.3:81327562-81327581 | None:intergenic | 35.0% | |
| TACATACCATGCATGACAAA+AGG | - | chr8.3:81327124-81327143 | None:intergenic | 35.0% | |
| TCCTTAATTTCCTTGTGTGA+TGG | - | chr8.3:81327370-81327389 | None:intergenic | 35.0% | |
| TTATGATGTACCATCACACA+AGG | + | chr8.3:81327357-81327376 | MS.gene91794:CDS | 35.0% | |
| ACCATGCATGACAAAAGGTT+TGG | - | chr8.3:81327119-81327138 | None:intergenic | 40.0% | |
| AGATATCAAGAATGGCAGTG+TGG | - | chr8.3:81326858-81326877 | None:intergenic | 40.0% | |
| ATCATAATGCTTACCAGGCA+AGG | - | chr8.3:81327344-81327363 | None:intergenic | 40.0% | |
| ATGATCATGAGTTACATGGG+AGG | + | chr8.3:81326596-81326615 | MS.gene91794:CDS | 40.0% | |
| GGGATGATCATGAGTTACAT+GGG | + | chr8.3:81326593-81326612 | MS.gene91794:CDS | 40.0% | |
| GGTACATCATAATGCTTACC+AGG | - | chr8.3:81327349-81327368 | None:intergenic | 40.0% | |
| GTTGGGACAAAGATAACATC+AGG | - | chr8.3:81327589-81327608 | None:intergenic | 40.0% | |
| ! | ACCAAACCTTTTGTCATGCA+TGG | + | chr8.3:81327115-81327134 | MS.gene91794:intron | 40.0% |
| ATCAAGAATGGCAGTGTGGA+AGG | - | chr8.3:81326854-81326873 | None:intergenic | 45.0% | |
| ATGAGTTACATGGGAGGATC+AGG | + | chr8.3:81326602-81326621 | MS.gene91794:CDS | 45.0% | |
| GCTATGGTAGCTAAGAGAAC+AGG | + | chr8.3:81327526-81327545 | MS.gene91794:CDS | 45.0% | |
| GGGGATGATCATGAGTTACA+TGG | + | chr8.3:81326592-81326611 | MS.gene91794:CDS | 45.0% | |
| ! | ACTATTAACATGGCCTTGCC+TGG | + | chr8.3:81327328-81327347 | MS.gene91794:CDS | 45.0% |
| !!! | AAATATGAATATTATTTTAA+AGG | + | chr8.3:81327009-81327028 | MS.gene91794:intron | 5.0% |
| CTCTTAGCTACCATAGCTGC+TGG | - | chr8.3:81327523-81327542 | None:intergenic | 50.0% | |
| TTAGCTACCATAGCTGCTGG+AGG | - | chr8.3:81327520-81327539 | None:intergenic | 50.0% | |
| AGTGGCACCTCCAGCAGCTA+TGG | + | chr8.3:81327510-81327529 | MS.gene91794:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 81326590 | 81327681 | 81326590 | ID=MS.gene91794 |
| chr8.3 | mRNA | 81326590 | 81327681 | 81326590 | ID=MS.gene91794.t1;Parent=MS.gene91794 |
| chr8.3 | exon | 81326590 | 81326623 | 81326590 | ID=MS.gene91794.t1.exon1;Parent=MS.gene91794.t1 |
| chr8.3 | CDS | 81326590 | 81326623 | 81326590 | ID=cds.MS.gene91794.t1;Parent=MS.gene91794.t1 |
| chr8.3 | exon | 81326764 | 81326881 | 81326764 | ID=MS.gene91794.t1.exon2;Parent=MS.gene91794.t1 |
| chr8.3 | CDS | 81326764 | 81326881 | 81326764 | ID=cds.MS.gene91794.t1;Parent=MS.gene91794.t1 |
| chr8.3 | exon | 81327327 | 81327681 | 81327327 | ID=MS.gene91794.t1.exon3;Parent=MS.gene91794.t1 |
| chr8.3 | CDS | 81327327 | 81327681 | 81327327 | ID=cds.MS.gene91794.t1;Parent=MS.gene91794.t1 |
| Gene Sequence |
| Protein sequence |
