Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92280.t1 | XP_003614456.2 | 92.1 | 821 | 55 | 4 | 1 | 811 | 1 | 821 | 0.00E+00 | 1489.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92280.t1 | B6SFA4 | 54.7 | 834 | 321 | 14 | 1 | 803 | 1 | 808 | 6.4e-244 | 845.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92280.t1 | G7JY95 | 92.1 | 821 | 55 | 4 | 1 | 811 | 1 | 821 | 0.0e+00 | 1489.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049666 | MS.gene92280 | 0.813829 | 2.10E-51 | -1.69E-46 |
| MS.gene049824 | MS.gene92280 | 0.84582 | 3.20E-59 | -1.69E-46 |
| MS.gene049825 | MS.gene92280 | 0.825539 | 4.44E-54 | -1.69E-46 |
| MS.gene049873 | MS.gene92280 | 0.821777 | 3.37E-53 | -1.69E-46 |
| MS.gene055808 | MS.gene92280 | 0.813884 | 2.04E-51 | -1.69E-46 |
| MS.gene056091 | MS.gene92280 | 0.804382 | 2.22E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene92280 | 0.810657 | 1.03E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92280.t1 | MTR_5g053930 | 92.448 | 821 | 52 | 4 | 1 | 811 | 1 | 821 | 0.0 | 1571 |
| MS.gene92280.t1 | MTR_1g052700 | 95.222 | 293 | 14 | 0 | 480 | 772 | 44 | 336 | 0.0 | 592 |
| MS.gene92280.t1 | MTR_8g020390 | 43.046 | 302 | 170 | 2 | 439 | 739 | 459 | 759 | 2.98e-71 | 251 |
| MS.gene92280.t1 | MTR_8g020390 | 43.046 | 302 | 170 | 2 | 439 | 739 | 747 | 1047 | 3.73e-70 | 251 |
| MS.gene92280.t1 | MTR_8g020390 | 43.046 | 302 | 170 | 2 | 439 | 739 | 964 | 1264 | 7.88e-70 | 251 |
| MS.gene92280.t1 | MTR_2g105760 | 38.056 | 360 | 188 | 6 | 421 | 749 | 1725 | 2080 | 1.38e-68 | 248 |
| MS.gene92280.t1 | MTR_2g105760 | 27.723 | 404 | 254 | 12 | 17 | 398 | 1231 | 1618 | 1.35e-29 | 127 |
| MS.gene92280.t1 | MTR_5g076300 | 38.235 | 306 | 182 | 7 | 440 | 741 | 552 | 854 | 8.63e-51 | 192 |
| MS.gene92280.t1 | MTR_5g076330 | 36.755 | 302 | 186 | 5 | 440 | 738 | 527 | 826 | 1.42e-48 | 186 |
| MS.gene92280.t1 | MTR_5g076440 | 35.621 | 306 | 191 | 6 | 440 | 741 | 541 | 844 | 2.37e-47 | 182 |
| MS.gene92280.t1 | MTR_5g076310 | 35.548 | 301 | 189 | 5 | 445 | 741 | 512 | 811 | 2.80e-47 | 181 |
| MS.gene92280.t1 | MTR_0189s0030 | 33.239 | 352 | 214 | 7 | 436 | 780 | 517 | 854 | 7.81e-47 | 182 |
| MS.gene92280.t1 | MTR_5g013820 | 30.211 | 427 | 257 | 12 | 339 | 741 | 500 | 909 | 5.09e-45 | 176 |
| MS.gene92280.t1 | MTR_3g064210 | 37.179 | 312 | 181 | 8 | 439 | 741 | 608 | 913 | 6.69e-45 | 175 |
| MS.gene92280.t1 | MTR_5g013820 | 30.211 | 427 | 257 | 12 | 339 | 741 | 500 | 909 | 1.01e-44 | 175 |
| MS.gene92280.t1 | MTR_3g064190 | 34.314 | 306 | 195 | 6 | 440 | 741 | 531 | 834 | 1.02e-44 | 174 |
| MS.gene92280.t1 | MTR_5g076380 | 34.539 | 304 | 191 | 7 | 445 | 743 | 535 | 835 | 2.76e-44 | 172 |
| MS.gene92280.t1 | MTR_3g436400 | 31.006 | 358 | 233 | 6 | 435 | 788 | 537 | 884 | 1.19e-43 | 172 |
| MS.gene92280.t1 | MTR_5g076320 | 33.893 | 298 | 182 | 6 | 445 | 738 | 559 | 845 | 6.25e-40 | 159 |
| MS.gene92280.t1 | MTR_5g076500 | 32.558 | 301 | 180 | 7 | 445 | 741 | 532 | 813 | 8.19e-38 | 152 |
| MS.gene92280.t1 | MTR_1g040155 | 28.634 | 454 | 257 | 12 | 321 | 729 | 450 | 881 | 2.39e-35 | 145 |
| MS.gene92280.t1 | MTR_7g092200 | 30.247 | 324 | 176 | 8 | 444 | 743 | 344 | 641 | 1.47e-31 | 131 |
| MS.gene92280.t1 | MTR_5g006890 | 28.916 | 415 | 229 | 16 | 359 | 744 | 186 | 563 | 3.09e-30 | 127 |
| MS.gene92280.t1 | MTR_2g049990 | 28.878 | 419 | 226 | 16 | 358 | 744 | 185 | 563 | 3.31e-30 | 127 |
| MS.gene92280.t1 | MTR_5g006890 | 29.157 | 415 | 228 | 17 | 359 | 744 | 445 | 822 | 6.15e-30 | 127 |
| MS.gene92280.t1 | MTR_2g049990 | 29.187 | 418 | 224 | 17 | 359 | 744 | 445 | 822 | 6.77e-30 | 127 |
| MS.gene92280.t1 | MTR_2g105760 | 27.723 | 404 | 254 | 12 | 17 | 398 | 1125 | 1512 | 2.04e-29 | 127 |
| MS.gene92280.t1 | MTR_2g105760 | 37.681 | 138 | 69 | 2 | 421 | 541 | 1619 | 1756 | 1.79e-19 | 94.7 |
| MS.gene92280.t1 | MTR_2g105760 | 27.723 | 404 | 254 | 12 | 17 | 398 | 1231 | 1618 | 2.24e-29 | 126 |
| MS.gene92280.t1 | MTR_2g105760 | 37.681 | 138 | 69 | 2 | 421 | 541 | 1725 | 1862 | 1.51e-19 | 95.1 |
| MS.gene92280.t1 | MTR_7g034575 | 28.032 | 371 | 211 | 12 | 360 | 715 | 952 | 1281 | 6.40e-19 | 92.8 |
| MS.gene92280.t1 | MTR_1g038815 | 22.691 | 379 | 248 | 13 | 435 | 791 | 1090 | 1445 | 3.95e-12 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92280.t1 | AT4G15570 | 56.116 | 793 | 301 | 12 | 1 | 762 | 1 | 777 | 0.0 | 889 |
| MS.gene92280.t1 | AT4G15570 | 56.116 | 793 | 301 | 12 | 1 | 762 | 1 | 777 | 0.0 | 889 |
| MS.gene92280.t1 | AT4G15570 | 58.430 | 688 | 256 | 8 | 98 | 762 | 8 | 688 | 0.0 | 825 |
| MS.gene92280.t1 | AT4G15570 | 58.430 | 688 | 256 | 8 | 98 | 762 | 8 | 688 | 0.0 | 825 |
| MS.gene92280.t1 | AT2G19120 | 42.904 | 303 | 171 | 2 | 439 | 740 | 744 | 1045 | 1.47e-70 | 252 |
| MS.gene92280.t1 | AT4G30100 | 42.904 | 303 | 171 | 2 | 439 | 740 | 907 | 1208 | 1.13e-68 | 248 |
| MS.gene92280.t1 | AT4G30100 | 42.904 | 303 | 171 | 2 | 439 | 740 | 907 | 1208 | 1.13e-68 | 248 |
| MS.gene92280.t1 | AT1G16800 | 39.233 | 339 | 180 | 5 | 440 | 754 | 1560 | 1896 | 1.27e-68 | 248 |
| MS.gene92280.t1 | AT1G16800 | 28.352 | 455 | 258 | 17 | 17 | 433 | 1044 | 1468 | 2.02e-32 | 136 |
| MS.gene92280.t1 | AT1G16800 | 39.233 | 339 | 180 | 5 | 440 | 754 | 1602 | 1938 | 1.52e-68 | 248 |
| MS.gene92280.t1 | AT1G16800 | 28.352 | 455 | 258 | 17 | 17 | 433 | 1086 | 1510 | 2.50e-32 | 136 |
| MS.gene92280.t1 | AT1G16800 | 39.233 | 339 | 180 | 5 | 440 | 754 | 1672 | 2008 | 2.21e-68 | 248 |
| MS.gene92280.t1 | AT1G16800 | 28.352 | 455 | 258 | 17 | 17 | 433 | 1156 | 1580 | 2.62e-32 | 136 |
| MS.gene92280.t1 | AT1G65810 | 36.066 | 305 | 188 | 5 | 440 | 741 | 526 | 826 | 1.84e-49 | 189 |
| MS.gene92280.t1 | AT1G65810 | 36.066 | 305 | 188 | 5 | 440 | 741 | 526 | 826 | 2.08e-49 | 189 |
| MS.gene92280.t1 | AT1G65810 | 36.066 | 305 | 188 | 5 | 440 | 741 | 526 | 826 | 2.46e-49 | 189 |
| MS.gene92280.t1 | AT5G37030 | 30.982 | 397 | 239 | 9 | 357 | 741 | 250 | 623 | 7.41e-48 | 181 |
| MS.gene92280.t1 | AT5G37165 | 34.076 | 314 | 197 | 8 | 435 | 741 | 79 | 389 | 9.55e-46 | 170 |
| MS.gene92280.t1 | AT5G47010 | 29.604 | 429 | 257 | 12 | 339 | 741 | 512 | 921 | 2.60e-45 | 176 |
| MS.gene92280.t1 | AT4G05540 | 35.211 | 284 | 178 | 5 | 440 | 719 | 402 | 683 | 3.31e-43 | 167 |
| MS.gene92280.t1 | AT5G37150 | 32.692 | 312 | 202 | 8 | 436 | 742 | 495 | 803 | 1.94e-42 | 166 |
| MS.gene92280.t1 | AT5G52090 | 31.511 | 311 | 206 | 7 | 437 | 742 | 332 | 640 | 2.04e-41 | 162 |
| MS.gene92280.t1 | AT5G37140 | 32.797 | 311 | 198 | 9 | 436 | 742 | 353 | 656 | 4.09e-40 | 158 |
| MS.gene92280.t1 | AT1G65780 | 34.375 | 320 | 195 | 8 | 433 | 743 | 530 | 843 | 8.17e-38 | 153 |
| MS.gene92280.t1 | AT5G35970 | 29.605 | 456 | 250 | 16 | 321 | 729 | 498 | 929 | 3.64e-37 | 150 |
| MS.gene92280.t1 | AT2G03270 | 28.982 | 452 | 226 | 16 | 348 | 743 | 224 | 636 | 3.75e-30 | 127 |
| MS.gene92280.t1 | AT1G05460 | 27.316 | 421 | 242 | 15 | 339 | 729 | 423 | 809 | 6.40e-29 | 124 |
| MS.gene92280.t1 | AT1G05460 | 27.316 | 421 | 242 | 15 | 339 | 729 | 423 | 809 | 8.82e-29 | 124 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6938 | gene | 73558 | 93120 | 73558 | ID=MS.gene92280 |
| 6938 | mRNA | 73558 | 93120 | 73558 | ID=MS.gene92280.t1;Parent=MS.gene92280 |
| 6938 | exon | 73558 | 73635 | 73558 | ID=MS.gene92280.t1.exon1;Parent=MS.gene92280.t1 |
| 6938 | CDS | 73558 | 73635 | 73558 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 73767 | 73917 | 73767 | ID=MS.gene92280.t1.exon2;Parent=MS.gene92280.t1 |
| 6938 | CDS | 73767 | 73917 | 73767 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 75489 | 75649 | 75489 | ID=MS.gene92280.t1.exon3;Parent=MS.gene92280.t1 |
| 6938 | CDS | 75489 | 75649 | 75489 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 75758 | 75931 | 75758 | ID=MS.gene92280.t1.exon4;Parent=MS.gene92280.t1 |
| 6938 | CDS | 75758 | 75931 | 75758 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 78879 | 79064 | 78879 | ID=MS.gene92280.t1.exon5;Parent=MS.gene92280.t1 |
| 6938 | CDS | 78879 | 79064 | 78879 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 79886 | 79921 | 79886 | ID=MS.gene92280.t1.exon6;Parent=MS.gene92280.t1 |
| 6938 | CDS | 79886 | 79921 | 79886 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 80560 | 80645 | 80560 | ID=MS.gene92280.t1.exon7;Parent=MS.gene92280.t1 |
| 6938 | CDS | 80560 | 80645 | 80560 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 80887 | 80937 | 80887 | ID=MS.gene92280.t1.exon8;Parent=MS.gene92280.t1 |
| 6938 | CDS | 80887 | 80937 | 80887 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 83334 | 83445 | 83334 | ID=MS.gene92280.t1.exon9;Parent=MS.gene92280.t1 |
| 6938 | CDS | 83334 | 83445 | 83334 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 83521 | 83626 | 83521 | ID=MS.gene92280.t1.exon10;Parent=MS.gene92280.t1 |
| 6938 | CDS | 83521 | 83626 | 83521 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 84493 | 84590 | 84493 | ID=MS.gene92280.t1.exon11;Parent=MS.gene92280.t1 |
| 6938 | CDS | 84493 | 84590 | 84493 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 85989 | 86099 | 85989 | ID=MS.gene92280.t1.exon12;Parent=MS.gene92280.t1 |
| 6938 | CDS | 85989 | 86099 | 85989 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 86185 | 86277 | 86185 | ID=MS.gene92280.t1.exon13;Parent=MS.gene92280.t1 |
| 6938 | CDS | 86185 | 86277 | 86185 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 86813 | 86866 | 86813 | ID=MS.gene92280.t1.exon14;Parent=MS.gene92280.t1 |
| 6938 | CDS | 86813 | 86866 | 86813 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 89307 | 89365 | 89307 | ID=MS.gene92280.t1.exon15;Parent=MS.gene92280.t1 |
| 6938 | CDS | 89307 | 89365 | 89307 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 89468 | 89552 | 89468 | ID=MS.gene92280.t1.exon16;Parent=MS.gene92280.t1 |
| 6938 | CDS | 89468 | 89552 | 89468 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 90779 | 91147 | 90779 | ID=MS.gene92280.t1.exon17;Parent=MS.gene92280.t1 |
| 6938 | CDS | 90779 | 91147 | 90779 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 92124 | 92246 | 92124 | ID=MS.gene92280.t1.exon18;Parent=MS.gene92280.t1 |
| 6938 | CDS | 92124 | 92246 | 92124 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 92331 | 92417 | 92331 | ID=MS.gene92280.t1.exon19;Parent=MS.gene92280.t1 |
| 6938 | CDS | 92331 | 92417 | 92331 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| 6938 | exon | 92905 | 93120 | 92905 | ID=MS.gene92280.t1.exon20;Parent=MS.gene92280.t1 |
| 6938 | CDS | 92905 | 93120 | 92905 | ID=cds.MS.gene92280.t1;Parent=MS.gene92280.t1 |
| Gene Sequence |
| Protein sequence |
