Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92297.t1 | XP_003610719.1 | 92.9 | 310 | 17 | 3 | 1 | 308 | 1 | 307 | 1.40E-161 | 578.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92297.t1 | B2A4J5 | 40.4 | 198 | 101 | 6 | 13 | 203 | 2 | 189 | 9.6e-31 | 135.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92297.t1 | G7K2E3 | 92.9 | 310 | 17 | 3 | 1 | 308 | 1 | 307 | 9.8e-162 | 578.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene051956 | MS.gene92297 | PPI |
| MS.gene76157 | MS.gene92297 | PPI |
| MS.gene92297 | MS.gene85230 | PPI |
| MS.gene92297 | MS.gene057003 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92297.t1 | MTR_5g006290 | 92.903 | 310 | 17 | 3 | 1 | 308 | 1 | 307 | 0.0 | 591 |
| MS.gene92297.t1 | MTR_5g006290 | 92.126 | 254 | 15 | 3 | 1 | 252 | 1 | 251 | 8.13e-172 | 477 |
| MS.gene92297.t1 | MTR_1g104500 | 70.000 | 310 | 87 | 4 | 1 | 308 | 1 | 306 | 1.40e-163 | 457 |
| MS.gene92297.t1 | MTR_5g006300 | 79.518 | 249 | 37 | 3 | 12 | 252 | 8 | 250 | 1.01e-143 | 406 |
| MS.gene92297.t1 | MTR_2g099720 | 35.604 | 323 | 168 | 6 | 14 | 299 | 53 | 372 | 5.51e-66 | 211 |
| MS.gene92297.t1 | MTR_1g104513 | 54.430 | 79 | 33 | 2 | 209 | 285 | 1 | 78 | 5.22e-19 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92297.t1 | AT4G14930 | 57.143 | 301 | 119 | 5 | 14 | 307 | 16 | 313 | 6.01e-122 | 352 |
| MS.gene92297.t1 | AT4G14930 | 56.475 | 278 | 110 | 6 | 14 | 283 | 16 | 290 | 2.72e-109 | 320 |
| MS.gene92297.t1 | AT1G72880 | 35.962 | 317 | 167 | 6 | 14 | 296 | 63 | 377 | 8.70e-62 | 201 |
| MS.gene92297.t1 | AT1G72880 | 35.962 | 317 | 167 | 6 | 14 | 296 | 63 | 377 | 8.70e-62 | 201 |
Find 72 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTCTTCCTTCGCTGTTTC+TGG | 0.110041 | 5.1:-1127221 | MS.gene92297:CDS |
| AGGTAGTGAGTGGCATAAAT+AGG | 0.271309 | 5.1:-1125930 | MS.gene92297:CDS |
| GCTGCCGACGAGAGGTGTTT+AGG | 0.296045 | 5.1:+1117749 | None:intergenic |
| CGCTCCTGGCTTAAGAGCTT+TGG | 0.339950 | 5.1:-1127375 | MS.gene92297:CDS |
| ACTGCTGATTTCTCAGTATC+AGG | 0.346261 | 5.1:+1127305 | None:intergenic |
| GGTAGTGAGTGGCATAAATA+GGG | 0.354531 | 5.1:-1125929 | MS.gene92297:CDS |
| GCCAAACAAGTTCATATTCA+TGG | 0.356932 | 5.1:-1127248 | MS.gene92297:CDS |
| CTGCCGACGAGAGGTGTTTA+GGG | 0.359627 | 5.1:+1117750 | None:intergenic |
| CCCGCTGATTGCACTTCTCT+TGG | 0.364382 | 5.1:-1127194 | MS.gene92297:CDS |
| CTCGCAAATAAAAGATGTTC+AGG | 0.372141 | 5.1:+1118332 | None:intergenic |
| ACCAGTACTGAGTTTATGAT+TGG | 0.387156 | 5.1:+1119775 | None:intergenic |
| TTGTAGCCAATCCTCAAAAT+AGG | 0.388766 | 5.1:+1117778 | None:intergenic |
| TCCATGAATATGAACTTGTT+TGG | 0.390536 | 5.1:+1127247 | None:intergenic |
| TCATATGAAATGGAGATAGA+AGG | 0.392459 | 5.1:+1120093 | None:intergenic |
| AACATACCAGTCATATGAAA+TGG | 0.413011 | 5.1:+1120083 | None:intergenic |
| TGAACTTGTTTGGCAGCTAT+AGG | 0.420107 | 5.1:+1127257 | None:intergenic |
| GACGGAAGGAAAGAGTGATT+TGG | 0.422993 | 5.1:+1127166 | None:intergenic |
| TATTCTATGTCCAGGGTTGA+AGG | 0.439800 | 5.1:-1119844 | MS.gene92297:intron |
| GGGTTACAAGCTAACGAGGC+AGG | 0.444468 | 5.1:-1118474 | MS.gene92297:CDS |
| ATTACTGTAACTGCTCTTGC+TGG | 0.445809 | 5.1:-1117830 | MS.gene92297:CDS |
| AGGAGAGGACTTACAAGATC+AGG | 0.448150 | 5.1:+1127143 | None:intergenic |
| TCTGTATCATCATCATAATC+AGG | 0.448757 | 5.1:+1117958 | None:intergenic |
| CTATCTCCATTTCATATGAC+TGG | 0.452640 | 5.1:-1120089 | MS.gene92297:intron |
| AGTAACAAAATCGATGGAAG+AGG | 0.459231 | 5.1:-1127456 | None:intergenic |
| TACCTTATGGTTAGCAACAT+TGG | 0.464242 | 5.1:+1119696 | None:intergenic |
| GTTTACTCAGGCACTGTAGC+TGG | 0.466587 | 5.1:-1120144 | MS.gene92297:CDS |
| GGGTAAAAGTATCTTTAAGA+TGG | 0.475283 | 5.1:-1118453 | MS.gene92297:CDS |
| AACCAATGTTGCTAACCATA+AGG | 0.509023 | 5.1:-1119698 | MS.gene92297:intron |
| GACGATGGAATCGACGCTCC+TGG | 0.513493 | 5.1:-1127389 | MS.gene92297:CDS |
| TCCAATCATAAACTCAGTAC+TGG | 0.516828 | 5.1:-1119776 | MS.gene92297:CDS |
| TTATGGTTAGCAACATTGGT+TGG | 0.519228 | 5.1:+1119700 | None:intergenic |
| AAAGTATCTTTAAGATGGGA+TGG | 0.524804 | 5.1:-1118448 | MS.gene92297:CDS |
| TACAGATCACAAATCTTTGA+AGG | 0.525967 | 5.1:-1117941 | MS.gene92297:CDS |
| TTCCCTTTCAGTGTTTACTC+AGG | 0.528508 | 5.1:-1120156 | MS.gene92297:intron |
| TATCATCATCATAATCAGGT+AGG | 0.530713 | 5.1:+1117962 | None:intergenic |
| GGTAAAAGTATCTTTAAGAT+GGG | 0.542844 | 5.1:-1118452 | MS.gene92297:CDS |
| CACTGTAGCTGGAGCTCGAG+AGG | 0.542909 | 5.1:-1120133 | MS.gene92297:CDS |
| AGAAGGGATATTGTTGAAGA+AGG | 0.544062 | 5.1:+1120110 | None:intergenic |
| GACACCAAAGCTCTTAAGCC+AGG | 0.545144 | 5.1:+1127371 | None:intergenic |
| TCCAAGAGAAGTGCAATCAG+CGG | 0.552211 | 5.1:+1127193 | None:intergenic |
| CATATGAAATGGAGATAGAA+GGG | 0.554398 | 5.1:+1120094 | None:intergenic |
| CCAAGAGAAGTGCAATCAGC+GGG | 0.556534 | 5.1:+1127194 | None:intergenic |
| AGAAACAGCGAAGGAAGAAA+CGG | 0.565756 | 5.1:+1127223 | None:intergenic |
| GATCACAAATCTTTGAAGGC+GGG | 0.566869 | 5.1:-1117937 | MS.gene92297:CDS |
| AATGCAAACTTGTGCAGCAA+GGG | 0.572061 | 5.1:+1119798 | None:intergenic |
| ATAAAGAAACAAAGCTACCC+TGG | 0.585747 | 5.1:-1119748 | MS.gene92297:CDS |
| AACATTAACAATAAGAGAGG+AGG | 0.591884 | 5.1:-1127431 | MS.gene92297:CDS |
| TTATTTGAACAGGTAGTGAG+TGG | 0.594935 | 5.1:-1125940 | MS.gene92297:intron |
| ATCATCATCATAATCAGGTA+GGG | 0.598459 | 5.1:+1117963 | None:intergenic |
| GTAACAAAATCGATGGAAGA+GGG | 0.604074 | 5.1:-1127455 | None:intergenic |
| GGTTACAAGCTAACGAGGCA+GGG | 0.604854 | 5.1:-1118473 | MS.gene92297:CDS |
| GGTAACATTAACAATAAGAG+AGG | 0.605178 | 5.1:-1127434 | MS.gene92297:CDS |
| GGAGAGGACTTACAAGATCA+GGG | 0.606857 | 5.1:+1127144 | None:intergenic |
| CAGTTGGTCACAGCATCACA+TGG | 0.611704 | 5.1:-1127286 | MS.gene92297:CDS |
| GAATGCAAACTTGTGCAGCA+AGG | 0.611800 | 5.1:+1119797 | None:intergenic |
| ATTATAAAGCTGCCGACGAG+AGG | 0.616494 | 5.1:+1117741 | None:intergenic |
| TGAAGGAAAGAGTAATCCAC+AGG | 0.618509 | 5.1:-1119827 | MS.gene92297:CDS |
| GGGAGTACCAGAAACAGCGA+AGG | 0.623127 | 5.1:+1127214 | None:intergenic |
| GATACTGAGAAATCAGCAGT+TGG | 0.641119 | 5.1:-1127302 | MS.gene92297:CDS |
| CAAGTCACCTCTGAGACAGA+AGG | 0.641811 | 5.1:-1118422 | MS.gene92297:CDS |
| TCTTCGTCCTTCTGTCTCAG+AGG | 0.641863 | 5.1:+1118415 | None:intergenic |
| CTTACAAGATCAGGGACGGA+AGG | 0.642273 | 5.1:+1127152 | None:intergenic |
| GCAGCAAGGGCAAAGTCCTG+TGG | 0.646391 | 5.1:+1119811 | None:intergenic |
| AGGACTTACAAGATCAGGGA+CGG | 0.647716 | 5.1:+1127148 | None:intergenic |
| TATCCCTAAACACCTCTCGT+CGG | 0.656627 | 5.1:-1117753 | MS.gene92297:CDS |
| GTATCTTTAAGATGGGATGG+AGG | 0.656769 | 5.1:-1118445 | MS.gene92297:CDS |
| ATAAATAGGGGTAGCAACTG+CGG | 0.656882 | 5.1:-1125916 | MS.gene92297:CDS |
| AGATCACAAATCTTTGAAGG+CGG | 0.659879 | 5.1:-1117938 | MS.gene92297:CDS |
| GTAGTGAGTGGCATAAATAG+GGG | 0.660329 | 5.1:-1125928 | MS.gene92297:CDS |
| TCTTGCTGGTCTCTCTCATG+CGG | 0.662540 | 5.1:-1117816 | MS.gene92297:CDS |
| ATTCTGATAACAAACGACGA+TGG | 0.666629 | 5.1:-1127404 | MS.gene92297:CDS |
| AAAAGGGTTACAAGCTAACG+AGG | 0.689847 | 5.1:-1118478 | MS.gene92297:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 1117742 | 1127465 | 1117742 | ID=MS.gene92297 |
| chr5.1 | mRNA | 1117742 | 1127465 | 1117742 | ID=MS.gene92297.t1;Parent=MS.gene92297 |
| chr5.1 | exon | 1127157 | 1127465 | 1127157 | ID=MS.gene92297.t1.exon1;Parent=MS.gene92297.t1 |
| chr5.1 | CDS | 1127157 | 1127465 | 1127157 | ID=cds.MS.gene92297.t1;Parent=MS.gene92297.t1 |
| chr5.1 | exon | 1125907 | 1125950 | 1125907 | ID=MS.gene92297.t1.exon2;Parent=MS.gene92297.t1 |
| chr5.1 | CDS | 1125907 | 1125950 | 1125907 | ID=cds.MS.gene92297.t1;Parent=MS.gene92297.t1 |
| chr5.1 | exon | 1120090 | 1120167 | 1120090 | ID=MS.gene92297.t1.exon3;Parent=MS.gene92297.t1 |
| chr5.1 | CDS | 1120090 | 1120167 | 1120090 | ID=cds.MS.gene92297.t1;Parent=MS.gene92297.t1 |
| chr5.1 | exon | 1119699 | 1119852 | 1119699 | ID=MS.gene92297.t1.exon4;Parent=MS.gene92297.t1 |
| chr5.1 | CDS | 1119699 | 1119852 | 1119699 | ID=cds.MS.gene92297.t1;Parent=MS.gene92297.t1 |
| chr5.1 | exon | 1118328 | 1118495 | 1118328 | ID=MS.gene92297.t1.exon5;Parent=MS.gene92297.t1 |
| chr5.1 | CDS | 1118328 | 1118495 | 1118328 | ID=cds.MS.gene92297.t1;Parent=MS.gene92297.t1 |
| chr5.1 | exon | 1117933 | 1117995 | 1117933 | ID=MS.gene92297.t1.exon6;Parent=MS.gene92297.t1 |
| chr5.1 | CDS | 1117933 | 1117995 | 1117933 | ID=cds.MS.gene92297.t1;Parent=MS.gene92297.t1 |
| chr5.1 | exon | 1117742 | 1117852 | 1117742 | ID=MS.gene92297.t1.exon7;Parent=MS.gene92297.t1 |
| chr5.1 | CDS | 1117742 | 1117852 | 1117742 | ID=cds.MS.gene92297.t1;Parent=MS.gene92297.t1 |
| Gene Sequence |
| Protein sequence |
