Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92683.t1 | XP_024635434.1 | 98.4 | 320 | 5 | 0 | 35 | 354 | 21 | 340 | 1.30E-184 | 655.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92683.t1 | Q9LM02 | 86.0 | 315 | 44 | 0 | 35 | 349 | 21 | 335 | 4.4e-165 | 582.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92683.t1 | B7FHU0 | 98.7 | 319 | 4 | 0 | 36 | 354 | 22 | 340 | 1.2e-184 | 655.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene92683 | MS.gene014381 | PPI |
| MS.gene92683 | MS.gene52804 | PPI |
| MS.gene92683 | MS.gene064797 | PPI |
| MS.gene92683 | MS.gene69360 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92683.t1 | MTR_3g032530 | 98.746 | 319 | 4 | 0 | 36 | 354 | 22 | 340 | 0.0 | 664 |
| MS.gene92683.t1 | MTR_3g032530 | 98.990 | 297 | 3 | 0 | 58 | 354 | 1 | 297 | 0.0 | 617 |
| MS.gene92683.t1 | MTR_3g114780 | 39.722 | 360 | 191 | 6 | 8 | 354 | 13 | 359 | 1.41e-86 | 265 |
| MS.gene92683.t1 | MTR_7g012060 | 53.933 | 89 | 12 | 2 | 162 | 221 | 65 | 153 | 4.60e-23 | 94.4 |
| MS.gene92683.t1 | MTR_6g069600 | 30.769 | 169 | 104 | 4 | 47 | 215 | 238 | 393 | 8.45e-14 | 72.8 |
| MS.gene92683.t1 | MTR_1g014690 | 25.806 | 186 | 127 | 2 | 59 | 234 | 69 | 253 | 8.99e-13 | 68.9 |
| MS.gene92683.t1 | MTR_1g014690 | 30.400 | 125 | 86 | 1 | 110 | 234 | 75 | 198 | 1.70e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92683.t1 | AT5G13710 | 86.032 | 315 | 44 | 0 | 35 | 349 | 21 | 335 | 0.0 | 588 |
| MS.gene92683.t1 | AT5G13710 | 86.032 | 315 | 44 | 0 | 35 | 349 | 21 | 335 | 0.0 | 588 |
| MS.gene92683.t1 | AT1G20330 | 43.831 | 308 | 157 | 3 | 47 | 351 | 62 | 356 | 3.93e-89 | 272 |
| MS.gene92683.t1 | AT1G76090 | 41.042 | 307 | 171 | 1 | 47 | 353 | 62 | 358 | 4.40e-86 | 264 |
| MS.gene92683.t1 | AT1G48600 | 31.176 | 170 | 105 | 5 | 97 | 265 | 256 | 414 | 9.72e-14 | 72.4 |
| MS.gene92683.t1 | AT1G48600 | 31.176 | 170 | 105 | 5 | 97 | 265 | 272 | 430 | 9.85e-14 | 72.4 |
| MS.gene92683.t1 | AT1G73600 | 35.714 | 112 | 70 | 1 | 104 | 215 | 277 | 386 | 1.68e-13 | 71.6 |
| MS.gene92683.t1 | AT1G73600 | 35.714 | 112 | 70 | 1 | 104 | 215 | 291 | 400 | 1.80e-13 | 71.6 |
| MS.gene92683.t1 | AT3G18000 | 31.288 | 163 | 103 | 4 | 103 | 265 | 277 | 430 | 1.98e-13 | 71.6 |
| MS.gene92683.t1 | AT1G64970 | 34.615 | 104 | 68 | 0 | 112 | 215 | 130 | 233 | 5.87e-13 | 69.7 |
Find 89 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATGTTGGTTGTGGTATT+GGG | 0.139062 | 3.1:-23420163 | MS.gene92683:CDS |
| AATCCAGAAATTTCTCTTAA+TGG | 0.184007 | 3.1:+23420137 | None:intergenic |
| TTGGATGTTGGTTGTGGTAT+TGG | 0.186951 | 3.1:-23420164 | MS.gene92683:CDS |
| CAGAAGCAATACCATATTTC+TGG | 0.200578 | 3.1:+23416993 | None:intergenic |
| TAAGAGAAATTTCTGGATTC+AGG | 0.201135 | 3.1:-23420133 | MS.gene92683:intron |
| GGACCATTAAGAGAAATTTC+TGG | 0.285956 | 3.1:-23420140 | MS.gene92683:CDS |
| TGCAGCTCGACATCTGTTAC+AGG | 0.301259 | 3.1:-23419674 | MS.gene92683:intron |
| ATGGACACCTTGATGCAAAA+TGG | 0.320698 | 3.1:+23420458 | None:intergenic |
| CAGGCACTCAATCGCAAAAC+TGG | 0.331030 | 3.1:-23419287 | MS.gene92683:CDS |
| CACTTCCTTGCTTTACAACT+TGG | 0.357247 | 3.1:-23420316 | MS.gene92683:CDS |
| GAAAGCTAATTATAGTGATA+TGG | 0.367025 | 3.1:-23421199 | MS.gene92683:intron |
| GGATGTTGGTTGTGGTATTG+GGG | 0.388964 | 3.1:-23420162 | MS.gene92683:CDS |
| ATACCTTTCCCCTGGTGATC+TGG | 0.394747 | 3.1:+23419633 | None:intergenic |
| GTGAAGGCTCTGGAGTTTGT+TGG | 0.407221 | 3.1:-23416670 | MS.gene92683:CDS |
| TAGAGCTTCAAGACACTTTG+TGG | 0.411789 | 3.1:+23417580 | None:intergenic |
| ATTCAGTTGCTTTCTGAAGC+AGG | 0.422845 | 3.1:+23414927 | None:intergenic |
| ACTTAGCAGCTTCCGTCTTA+CGG | 0.429065 | 3.1:-23417034 | MS.gene92683:CDS |
| AATATTTCACATACAGCATC+TGG | 0.437219 | 3.1:+23418110 | None:intergenic |
| TGAACCCTTTGACTCCCCTT+CGG | 0.452704 | 3.1:+23416640 | None:intergenic |
| AAAGATGAAAACCTTCTGTC+CGG | 0.459180 | 3.1:+23420285 | None:intergenic |
| AGCTTCCGTCTTACGGCTGT+TGG | 0.465882 | 3.1:-23417027 | MS.gene92683:CDS |
| AGTGAATCAGAGGGAGGCAG+AGG | 0.472649 | 3.1:-23423854 | MS.gene92683:CDS |
| TCTTCCGATGCAGATGGGTA+GGG | 0.476834 | 3.1:-23420372 | MS.gene92683:intron |
| CAGCGGAGTGAGATGGTGTC+AGG | 0.479428 | 3.1:-23423900 | MS.gene92683:CDS |
| ATACCATATTTCTGGTGAAA+AGG | 0.482576 | 3.1:+23417001 | None:intergenic |
| CTGCAGGCAGAAATTGAGAT+TGG | 0.490804 | 3.1:-23417629 | MS.gene92683:intron |
| GCTGCAGAGGGTCTAGTTGA+AGG | 0.505379 | 3.1:-23416601 | MS.gene92683:CDS |
| ACATTTGTAGGTGAAGGCTC+TGG | 0.507945 | 3.1:-23416680 | MS.gene92683:intron |
| GAGAAGTATCATGCATCCTA+TGG | 0.508363 | 3.1:-23421240 | MS.gene92683:CDS |
| CTTCCGATGCAGATGGGTAG+GGG | 0.508765 | 3.1:-23420371 | MS.gene92683:intron |
| TTGCACCGAAGGGGAGTCAA+AGG | 0.512975 | 3.1:-23416645 | MS.gene92683:CDS |
| TCAGGAGAGAATGGCGGCGA+GGG | 0.513895 | 3.1:-23423882 | MS.gene92683:CDS |
| TTTGTATGTGAAGGTCATAT+GGG | 0.514447 | 3.1:-23417121 | MS.gene92683:intron |
| GGTCATATGGGAGAAAGATC+TGG | 0.515289 | 3.1:-23417109 | MS.gene92683:CDS |
| GAGATGGTGTCAGGAGAGAA+TGG | 0.521088 | 3.1:-23423891 | MS.gene92683:CDS |
| TACAGCATCTGGTGCATGGC+AGG | 0.521675 | 3.1:+23418121 | None:intergenic |
| TAAGCAGCAAAACATTGGCC+AGG | 0.524306 | 3.1:+23417789 | None:intergenic |
| GAGAAGTGACTCTTGTCTAA+AGG | 0.526724 | 3.1:+23417057 | None:intergenic |
| GCAGAAATTGAGATTGGTGA+TGG | 0.534551 | 3.1:-23417623 | MS.gene92683:CDS |
| GGTTCAAGATTTCCTTGAGA+AGG | 0.535966 | 3.1:-23416623 | MS.gene92683:CDS |
| TTCTTCCGATGCAGATGGGT+AGG | 0.537612 | 3.1:-23420373 | MS.gene92683:intron |
| TCAGAGGGAGGCAGAGGACA+AGG | 0.538844 | 3.1:-23423848 | MS.gene92683:CDS |
| GACCCTCTGCAGCCTTCTCA+AGG | 0.541641 | 3.1:+23416611 | None:intergenic |
| TTTGTGGTCAATCTAATGTC+AGG | 0.541951 | 3.1:+23417596 | None:intergenic |
| TTCATCCTCTTGACCTCCAT+AGG | 0.544369 | 3.1:+23421224 | None:intergenic |
| CTGACACCATCTCACTCCGC+TGG | 0.545163 | 3.1:+23423901 | None:intergenic |
| AAAGGCAAGTACCACGGAAG+AGG | 0.547176 | 3.1:+23417075 | None:intergenic |
| CTTGAAGCTCTAAAGCAAGC+AGG | 0.547652 | 3.1:-23417569 | MS.gene92683:CDS |
| TGGTCTTAAGCCCGGACAGA+AGG | 0.551005 | 3.1:-23420296 | MS.gene92683:intron |
| TTACAACTTGGTCTTAAGCC+CGG | 0.554828 | 3.1:-23420304 | MS.gene92683:CDS |
| TTCCTTGAGAAGGCTGCAGA+GGG | 0.556231 | 3.1:-23416613 | MS.gene92683:CDS |
| AAGATGAAAACCTTCTGTCC+GGG | 0.556269 | 3.1:+23420286 | None:intergenic |
| GGCGTCCAACAGCCGTAAGA+CGG | 0.556704 | 3.1:+23417022 | None:intergenic |
| ATTCATAAGCAGCAAAACAT+TGG | 0.559750 | 3.1:+23417784 | None:intergenic |
| ATAATGAGTACCAGATCACC+AGG | 0.560207 | 3.1:-23419643 | MS.gene92683:CDS |
| GTTTGTTGGACTTGCACCGA+AGG | 0.560690 | 3.1:-23416656 | MS.gene92683:CDS |
| ATGGAGGTCAAGAGGATGAA+AGG | 0.562038 | 3.1:-23421221 | MS.gene92683:CDS |
| GTCTTTCCAGCGGAGTGAGA+TGG | 0.564591 | 3.1:-23423907 | MS.gene92683:CDS |
| GGTATGAGAACGCCGGCGAA+TGG | 0.566115 | 3.1:-23423929 | MS.gene92683:CDS |
| GAGGGAGAGAAGTGAATCAG+AGG | 0.570113 | 3.1:-23423864 | MS.gene92683:CDS |
| CACATACAGCATCTGGTGCA+TGG | 0.575106 | 3.1:+23418117 | None:intergenic |
| TCAAAACTATTGTCTTCGAA+TGG | 0.577172 | 3.1:+23418167 | None:intergenic |
| CAGAAATTGAGATTGGTGAT+GGG | 0.583752 | 3.1:-23417622 | MS.gene92683:CDS |
| TTTCCTTGAGAAGGCTGCAG+AGG | 0.588645 | 3.1:-23416614 | MS.gene92683:CDS |
| GATGTTGGTTGTGGTATTGG+GGG | 0.596492 | 3.1:-23420161 | MS.gene92683:CDS |
| TAAGACCAAGTTGTAAAGCA+AGG | 0.600321 | 3.1:+23420311 | None:intergenic |
| GTACCAGATCACCAGGGGAA+AGG | 0.600780 | 3.1:-23419636 | MS.gene92683:intron |
| AGGGAGAGAAGTGAATCAGA+GGG | 0.601619 | 3.1:-23423863 | MS.gene92683:CDS |
| TTTGTTGGACTTGCACCGAA+GGG | 0.601946 | 3.1:-23416655 | MS.gene92683:CDS |
| TGCATCCTATGGAGGTCAAG+AGG | 0.610680 | 3.1:-23421229 | MS.gene92683:CDS |
| CAAAACACATACCTTTCCCC+TGG | 0.613010 | 3.1:+23419625 | None:intergenic |
| TGCACCGAAGGGGAGTCAAA+GGG | 0.614024 | 3.1:-23416644 | MS.gene92683:CDS |
| AGCATCTGGTGCATGGCAGG+TGG | 0.619604 | 3.1:+23418124 | None:intergenic |
| GTCAGGAGAGAATGGCGGCG+AGG | 0.623602 | 3.1:-23423883 | MS.gene92683:CDS |
| GAGGCAGAGGACAAGGACAG+AGG | 0.633011 | 3.1:-23423841 | MS.gene92683:intron |
| CATCAGACTCTCCTCTTCCG+TGG | 0.635051 | 3.1:-23417086 | MS.gene92683:CDS |
| GCAGAGGGTCTAGTTGAAGG+TGG | 0.639355 | 3.1:-23416598 | MS.gene92683:CDS |
| TTGTTGGACTTGCACCGAAG+GGG | 0.642356 | 3.1:-23416654 | MS.gene92683:CDS |
| CCGGCGAATGGTCTTTCCAG+CGG | 0.651106 | 3.1:-23423917 | MS.gene92683:CDS |
| AAGTATCATGCATCCTATGG+AGG | 0.651186 | 3.1:-23421237 | MS.gene92683:CDS |
| CCGCTGGAAAGACCATTCGC+CGG | 0.656082 | 3.1:+23423917 | None:intergenic |
| GAGAGAAGTGAATCAGAGGG+AGG | 0.656529 | 3.1:-23423860 | MS.gene92683:CDS |
| CAAGACTTGCAACTTCGTCA+AGG | 0.656952 | 3.1:-23419258 | MS.gene92683:intron |
| ACTCAATCGCAAAACTGGAG+TGG | 0.680106 | 3.1:-23419282 | MS.gene92683:CDS |
| TAAGGTGGGTATGAGAACGC+CGG | 0.683162 | 3.1:-23423936 | None:intergenic |
| TTGTCTAAAGGCAAGTACCA+CGG | 0.688137 | 3.1:+23417069 | None:intergenic |
| AATGAGTACCAGATCACCAG+GGG | 0.700086 | 3.1:-23419641 | MS.gene92683:CDS |
| ATGGTGTCAGGAGAGAATGG+CGG | 0.721835 | 3.1:-23423888 | MS.gene92683:CDS |
| TAATGAGTACCAGATCACCA+GGG | 0.727702 | 3.1:-23419642 | MS.gene92683:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 23414929 | 23423948 | 23414929 | ID=MS.gene92683 |
| chr3.1 | mRNA | 23414929 | 23423948 | 23414929 | ID=MS.gene92683.t1;Parent=MS.gene92683 |
| chr3.1 | exon | 23423842 | 23423948 | 23423842 | ID=MS.gene92683.t1.exon1;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23423842 | 23423948 | 23423842 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23421200 | 23421266 | 23421200 | ID=MS.gene92683.t1.exon2;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23421200 | 23421266 | 23421200 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23420466 | 23420539 | 23420466 | ID=MS.gene92683.t1.exon3;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23420466 | 23420539 | 23420466 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23420297 | 23420381 | 23420297 | ID=MS.gene92683.t1.exon4;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23420297 | 23420381 | 23420297 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23420134 | 23420189 | 23420134 | ID=MS.gene92683.t1.exon5;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23420134 | 23420189 | 23420134 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23419637 | 23419691 | 23419637 | ID=MS.gene92683.t1.exon6;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23419637 | 23419691 | 23419637 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23419259 | 23419306 | 23419259 | ID=MS.gene92683.t1.exon7;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23419259 | 23419306 | 23419259 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23418124 | 23418207 | 23418124 | ID=MS.gene92683.t1.exon8;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23418124 | 23418207 | 23418124 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23417731 | 23417847 | 23417731 | ID=MS.gene92683.t1.exon9;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23417731 | 23417847 | 23417731 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23417562 | 23417645 | 23417562 | ID=MS.gene92683.t1.exon10;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23417562 | 23417645 | 23417562 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23417005 | 23417130 | 23417005 | ID=MS.gene92683.t1.exon11;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23417005 | 23417130 | 23417005 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23416595 | 23416692 | 23416595 | ID=MS.gene92683.t1.exon12;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23416595 | 23416692 | 23416595 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| chr3.1 | exon | 23414929 | 23414992 | 23414929 | ID=MS.gene92683.t1.exon13;Parent=MS.gene92683.t1 |
| chr3.1 | CDS | 23414929 | 23414992 | 23414929 | ID=cds.MS.gene92683.t1;Parent=MS.gene92683.t1 |
| Gene Sequence |
| Protein sequence |
