Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92946.t1 | XP_003592570.1 | 98.8 | 173 | 2 | 0 | 1 | 173 | 1 | 173 | 6.60E-89 | 336.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92946.t1 | G7IAV0 | 98.8 | 173 | 2 | 0 | 1 | 173 | 1 | 173 | 4.7e-89 | 336.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene92946 | 0.835984 | 1.22E-56 | -1.69E-46 |
| MS.gene051226 | MS.gene92946 | 0.819613 | 1.06E-52 | -1.69E-46 |
| MS.gene051837 | MS.gene92946 | 0.847158 | 1.38E-59 | -1.69E-46 |
| MS.gene057093 | MS.gene92946 | 0.822344 | 2.49E-53 | -1.69E-46 |
| MS.gene057432 | MS.gene92946 | 0.805835 | 1.10E-49 | -1.69E-46 |
| MS.gene06061 | MS.gene92946 | 0.8483 | 6.69E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92946.t1 | MTR_1g108640 | 98.844 | 173 | 2 | 0 | 1 | 173 | 1 | 173 | 9.58e-125 | 348 |
| MS.gene92946.t1 | MTR_2g105800 | 46.452 | 155 | 69 | 4 | 18 | 170 | 13 | 155 | 6.14e-37 | 125 |
| MS.gene92946.t1 | MTR_8g107420 | 33.708 | 178 | 98 | 4 | 1 | 171 | 1 | 165 | 5.44e-17 | 74.3 |
| MS.gene92946.t1 | MTR_4g068850 | 26.667 | 105 | 75 | 1 | 20 | 124 | 21 | 123 | 1.60e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92946.t1 | AT3G03280 | 41.111 | 180 | 81 | 6 | 1 | 170 | 1 | 165 | 5.80e-36 | 123 |
| MS.gene92946.t1 | AT5G17350 | 40.957 | 188 | 87 | 6 | 1 | 170 | 1 | 182 | 1.98e-32 | 114 |
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGATCAAAGAAACCTATTT+TGG | 0.162510 | 1.2:-80698620 | MS.gene92946:CDS |
| TTCCTTTATCTTGACCTAAT+TGG | 0.198618 | 1.2:+80698580 | None:intergenic |
| GCAGATGAAGAATTGGAATT+TGG | 0.234414 | 1.2:-80698886 | MS.gene92946:CDS |
| CGGCTGAACACGTGTCTTTC+CGG | 0.234431 | 1.2:+80698756 | None:intergenic |
| TGGAATCAGGGTTTAGTTAT+AGG | 0.257374 | 1.2:-80698654 | MS.gene92946:CDS |
| AGTTTGGAAGGTGTGGAATC+AGG | 0.279397 | 1.2:-80698667 | MS.gene92946:CDS |
| TCATTTATAGTCTCCAAAAT+AGG | 0.290361 | 1.2:+80698607 | None:intergenic |
| GAGTTGACTAGGAAATAATT+TGG | 0.307504 | 1.2:+80698949 | None:intergenic |
| TCTTGCTGCAGATGAAGAAT+TGG | 0.387242 | 1.2:-80698893 | MS.gene92946:CDS |
| AACCAATTAGGTCAAGATAA+AGG | 0.404180 | 1.2:-80698582 | None:intergenic |
| GTTTGGAAGGTGTGGAATCA+GGG | 0.418181 | 1.2:-80698666 | MS.gene92946:CDS |
| AATTGTTTCACTTCTCCTGT+TGG | 0.426516 | 1.2:+80699012 | None:intergenic |
| CTATAAATGAAGAACCAATT+AGG | 0.456816 | 1.2:-80698594 | MS.gene92946:CDS |
| ATGTGTTCCGAGGTTGAGTT+TGG | 0.464828 | 1.2:-80698683 | MS.gene92946:CDS |
| TGTTCAGCCGGATGAGAGTT+CGG | 0.467508 | 1.2:-80698743 | MS.gene92946:CDS |
| ACAGAATTGAGTCTTCTCAT+AGG | 0.502486 | 1.2:+80698844 | None:intergenic |
| CCTCATCAACGGTGGTGCTA+AGG | 0.505204 | 1.2:+80699062 | None:intergenic |
| CAGCACCGCCATGTCAGCTC+CGG | 0.507579 | 1.2:+80698816 | None:intergenic |
| GTTTCACTTCTCCTGTTGGA+AGG | 0.509220 | 1.2:+80699016 | None:intergenic |
| ACAAGAGTAATCCTTCCAAC+AGG | 0.510852 | 1.2:-80699027 | MS.gene92946:CDS |
| TGTGGTCACCGGAGCTGACA+TGG | 0.524219 | 1.2:-80698824 | MS.gene92946:CDS |
| CGGAAAGACACGTGTTCAGC+CGG | 0.528707 | 1.2:-80698755 | MS.gene92946:CDS |
| TTCACTTCCGAACTCTCATC+CGG | 0.533475 | 1.2:+80698736 | None:intergenic |
| GGATGAGAGTTCGGAAGTGA+AGG | 0.539299 | 1.2:-80698734 | MS.gene92946:CDS |
| CGGCGAATTCGGCTGCGAAG+CGG | 0.542084 | 1.2:-80698786 | MS.gene92946:CDS |
| GCTGCGAAGCGGTTACGTGC+CGG | 0.545063 | 1.2:-80698775 | MS.gene92946:CDS |
| AATTCTTCATCTGCAGCAAG+AGG | 0.547180 | 1.2:+80698895 | None:intergenic |
| AGACTCAATTCTGTGGTCAC+CGG | 0.558052 | 1.2:-80698835 | MS.gene92946:CDS |
| GAAGAGATCGAGAGTTGACT+AGG | 0.559968 | 1.2:+80698938 | None:intergenic |
| GAGGTTGAGTTTGGAAGGTG+TGG | 0.563066 | 1.2:-80698674 | MS.gene92946:CDS |
| CCTTAGCACCACCGTTGATG+AGG | 0.584450 | 1.2:-80699062 | MS.gene92946:CDS |
| GTTCCGAGGTTGAGTTTGGA+AGG | 0.607016 | 1.2:-80698679 | MS.gene92946:CDS |
| GATGAGAGTTCGGAAGTGAA+GGG | 0.620669 | 1.2:-80698733 | MS.gene92946:CDS |
| ACACCTTCCAAACTCAACCT+CGG | 0.627609 | 1.2:+80698676 | None:intergenic |
| TATGAGAAGACTCAATTCTG+TGG | 0.636561 | 1.2:-80698842 | MS.gene92946:CDS |
| TTTGCGTTCCTCATCAACGG+TGG | 0.639490 | 1.2:+80699054 | None:intergenic |
| ATCAAAATGAATGTGTTCCG+AGG | 0.670622 | 1.2:-80698693 | MS.gene92946:CDS |
| GGTCACCGGAGCTGACATGG+CGG | 0.787053 | 1.2:-80698821 | MS.gene92946:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTATAAATGAAGAACCAATT+AGG | - | chr1.2:80699075-80699094 | MS.gene92946:CDS | 25.0% |
| ! | TCATTTATAGTCTCCAAAAT+AGG | + | chr1.2:80699065-80699084 | None:intergenic | 25.0% |
| !! | TAGATCAAAGAAACCTATTT+TGG | - | chr1.2:80699049-80699068 | MS.gene92946:CDS | 25.0% |
| GAGTTGACTAGGAAATAATT+TGG | + | chr1.2:80698723-80698742 | None:intergenic | 30.0% | |
| AATTGTTTCACTTCTCCTGT+TGG | + | chr1.2:80698660-80698679 | None:intergenic | 35.0% | |
| ACAGAATTGAGTCTTCTCAT+AGG | + | chr1.2:80698828-80698847 | None:intergenic | 35.0% | |
| ATCAAAATGAATGTGTTCCG+AGG | - | chr1.2:80698976-80698995 | MS.gene92946:CDS | 35.0% | |
| GCAGATGAAGAATTGGAATT+TGG | - | chr1.2:80698783-80698802 | MS.gene92946:CDS | 35.0% | |
| TATGAGAAGACTCAATTCTG+TGG | - | chr1.2:80698827-80698846 | MS.gene92946:CDS | 35.0% | |
| TGGAATCAGGGTTTAGTTAT+AGG | - | chr1.2:80699015-80699034 | MS.gene92946:CDS | 35.0% | |
| AATTCTTCATCTGCAGCAAG+AGG | + | chr1.2:80698777-80698796 | None:intergenic | 40.0% | |
| ACAAGAGTAATCCTTCCAAC+AGG | - | chr1.2:80698642-80698661 | MS.gene92946:CDS | 40.0% | |
| TCTTGCTGCAGATGAAGAAT+TGG | - | chr1.2:80698776-80698795 | MS.gene92946:CDS | 40.0% | |
| ACACCTTCCAAACTCAACCT+CGG | + | chr1.2:80698996-80699015 | None:intergenic | 45.0% | |
| AGACTCAATTCTGTGGTCAC+CGG | - | chr1.2:80698834-80698853 | MS.gene92946:CDS | 45.0% | |
| ATGTGTTCCGAGGTTGAGTT+TGG | - | chr1.2:80698986-80699005 | MS.gene92946:CDS | 45.0% | |
| GAAGAGATCGAGAGTTGACT+AGG | + | chr1.2:80698734-80698753 | None:intergenic | 45.0% | |
| GATGAGAGTTCGGAAGTGAA+GGG | - | chr1.2:80698936-80698955 | MS.gene92946:CDS | 45.0% | |
| GTTTCACTTCTCCTGTTGGA+AGG | + | chr1.2:80698656-80698675 | None:intergenic | 45.0% | |
| TTCACTTCCGAACTCTCATC+CGG | + | chr1.2:80698936-80698955 | None:intergenic | 45.0% | |
| ! | AGTTTGGAAGGTGTGGAATC+AGG | - | chr1.2:80699002-80699021 | MS.gene92946:CDS | 45.0% |
| ! | GCTTTTGCGTTCCTCATCAA+CGG | + | chr1.2:80698621-80698640 | None:intergenic | 45.0% |
| ! | GTTTGGAAGGTGTGGAATCA+GGG | - | chr1.2:80699003-80699022 | MS.gene92946:CDS | 45.0% |
| GAGGTTGAGTTTGGAAGGTG+TGG | - | chr1.2:80698995-80699014 | MS.gene92946:CDS | 50.0% | |
| GGATGAGAGTTCGGAAGTGA+AGG | - | chr1.2:80698935-80698954 | MS.gene92946:CDS | 50.0% | |
| GTTCCGAGGTTGAGTTTGGA+AGG | - | chr1.2:80698990-80699009 | MS.gene92946:CDS | 50.0% | |
| TGTTCAGCCGGATGAGAGTT+CGG | - | chr1.2:80698926-80698945 | MS.gene92946:CDS | 50.0% | |
| TTTGCGTTCCTCATCAACGG+TGG | + | chr1.2:80698618-80698637 | None:intergenic | 50.0% | |
| !!! | GCTGTTTTTGGCGGCGAATT+CGG | - | chr1.2:80698872-80698891 | MS.gene92946:CDS | 50.0% |
| !!! | TGACATGGCGGTGCTGTTTT+TGG | - | chr1.2:80698860-80698879 | MS.gene92946:CDS | 50.0% |
| CCTTAGCACCACCGTTGATG+AGG | - | chr1.2:80698607-80698626 | MS.gene92946:CDS | 55.0% | |
| CGGAAAGACACGTGTTCAGC+CGG | - | chr1.2:80698914-80698933 | MS.gene92946:CDS | 55.0% | |
| CGGCTGAACACGTGTCTTTC+CGG | + | chr1.2:80698916-80698935 | None:intergenic | 55.0% | |
| ! | CCTCATCAACGGTGGTGCTA+AGG | + | chr1.2:80698610-80698629 | None:intergenic | 55.0% |
| !!! | CATGGCGGTGCTGTTTTTGG+CGG | - | chr1.2:80698863-80698882 | MS.gene92946:CDS | 55.0% |
| TGTGGTCACCGGAGCTGACA+TGG | - | chr1.2:80698845-80698864 | MS.gene92946:CDS | 60.0% | |
| CAGCACCGCCATGTCAGCTC+CGG | + | chr1.2:80698856-80698875 | None:intergenic | 65.0% | |
| CGGCGAATTCGGCTGCGAAG+CGG | - | chr1.2:80698883-80698902 | MS.gene92946:CDS | 65.0% | |
| GCTGCGAAGCGGTTACGTGC+CGG | - | chr1.2:80698894-80698913 | MS.gene92946:CDS | 65.0% | |
| GGTCACCGGAGCTGACATGG+CGG | - | chr1.2:80698848-80698867 | MS.gene92946:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 80698585 | 80699106 | 80698585 | ID=MS.gene92946 |
| chr1.2 | mRNA | 80698585 | 80699106 | 80698585 | ID=MS.gene92946.t1;Parent=MS.gene92946 |
| chr1.2 | exon | 80698585 | 80699106 | 80698585 | ID=MS.gene92946.t1.exon1;Parent=MS.gene92946.t1 |
| chr1.2 | CDS | 80698585 | 80699106 | 80698585 | ID=cds.MS.gene92946.t1;Parent=MS.gene92946.t1 |
| Gene Sequence |
| Protein sequence |
