Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93432.t1 | XP_024635709.1 | 97.3 | 37 | 1 | 0 | 38 | 74 | 149 | 185 | 2.40E-10 | 75.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93432.t1 | O04331 | 83.3 | 36 | 6 | 0 | 39 | 74 | 151 | 186 | 1.0e-09 | 64.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93432.t1 | B7FJH0 | 97.3 | 37 | 1 | 0 | 38 | 74 | 149 | 185 | 1.7e-10 | 75.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049968 | MS.gene93432 | 0.814869 | 1.24E-51 | -1.69E-46 |
| MS.gene052447 | MS.gene93432 | 0.806041 | 9.97E-50 | -1.69E-46 |
| MS.gene056272 | MS.gene93432 | 0.803815 | 2.91E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene93432 | -0.809941 | 1.48E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93432.t1 | MTR_3g008250 | 92.308 | 39 | 3 | 0 | 38 | 76 | 149 | 187 | 1.17e-18 | 79.0 |
| MS.gene93432.t1 | MTR_8g046300 | 84.615 | 39 | 6 | 0 | 38 | 76 | 150 | 188 | 1.97e-16 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93432.t1 | AT3G27280 | 65.909 | 44 | 15 | 0 | 38 | 81 | 150 | 193 | 1.04e-14 | 68.2 |
| MS.gene93432.t1 | AT3G27280 | 65.909 | 44 | 15 | 0 | 38 | 81 | 150 | 193 | 1.04e-14 | 68.2 |
| MS.gene93432.t1 | AT5G40770 | 76.923 | 39 | 9 | 0 | 39 | 77 | 151 | 189 | 5.50e-14 | 66.2 |
| MS.gene93432.t1 | AT5G14300 | 69.444 | 36 | 11 | 0 | 38 | 73 | 116 | 151 | 1.03e-11 | 59.7 |
Find 25 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATTACTCATTTGTCTTA+TGG | 0.196763 | 6.4:-30891418 | MS.gene93432:CDS |
| CTCAGGTTTCAGCTCTTGTT+AGG | 0.276412 | 6.4:-30891492 | MS.gene93432:intron |
| CCAGTCTCAGTTGCGGCAGC+AGG | 0.346506 | 6.4:-30891087 | MS.gene93432:CDS |
| CCTGCTGCCGCAACTGAGAC+TGG | 0.399465 | 6.4:+30891087 | None:intergenic |
| CAGTCTCAGTTGCGGCAGCA+GGG | 0.445177 | 6.4:-30891086 | MS.gene93432:CDS |
| TCAGGTTTCAGCTCTTGTTA+GGG | 0.453338 | 6.4:-30891491 | MS.gene93432:intron |
| CTTATGGTGGTCAATTCTCT+AGG | 0.458610 | 6.4:-30891402 | MS.gene93432:intron |
| AAGAAGAATATTGAAATCCT+TGG | 0.459418 | 6.4:+30891450 | None:intergenic |
| TTAATTTGTAGAATTATATG+AGG | 0.459456 | 6.4:-30890705 | MS.gene93432:intron |
| AGAACAAATCGAGTTGTTGA+AGG | 0.473358 | 6.4:+30891620 | None:intergenic |
| ATTACTCATTTGTCTTATGG+TGG | 0.489261 | 6.4:-30891415 | MS.gene93432:CDS |
| TTAGCTGGTTTAGGTGCTGT+TGG | 0.493723 | 6.4:-30891125 | MS.gene93432:intron |
| GCTGTTGGTGCCTTGCTGTC+CGG | 0.495768 | 6.4:-30891110 | MS.gene93432:CDS |
| TAGGGATAGTCTTGTGAGAA+GGG | 0.523460 | 6.4:-30891473 | MS.gene93432:CDS |
| TTAGGGATAGTCTTGTGAGA+AGG | 0.533036 | 6.4:-30891474 | MS.gene93432:CDS |
| CTGCCGCAACTGAGACTGGC+CGG | 0.539531 | 6.4:+30891091 | None:intergenic |
| AGTCTCAGTTGCGGCAGCAG+GGG | 0.543370 | 6.4:-30891085 | MS.gene93432:intron |
| GTTCAAGAAGAAGAAACTCA+TGG | 0.549247 | 6.4:-30890671 | MS.gene93432:CDS |
| CTGAGACTGGCCGGACAGCA+AGG | 0.564285 | 6.4:+30891100 | None:intergenic |
| TCACGTATGCTTCAGTTGAA+CGG | 0.599762 | 6.4:-30891694 | None:intergenic |
| TAGTCTTGTGAGAAGGGCCA+AGG | 0.601437 | 6.4:-30891467 | MS.gene93432:CDS |
| TGTCCGGCCAGTCTCAGTTG+CGG | 0.635165 | 6.4:-30891094 | MS.gene93432:CDS |
| AAGAGAGAGAATGAGATCTG+AGG | 0.650225 | 6.4:+30891668 | None:intergenic |
| TAGACAAGCATCAAACAGTG+TGG | 0.688442 | 6.4:-30890645 | MS.gene93432:CDS |
| CGTATGCTTCAGTTGAACGG+CGG | 0.779615 | 6.4:-30891691 | MS.gene93432:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAGAAATTAAAGTAGTTA+TGG | - | chr6.4:30891034-30891053 | MS.gene93432:intron | 15.0% |
| !!! | TATATTTCTTTCTTTTTTGT+TGG | - | chr6.4:30890945-30890964 | MS.gene93432:intron | 15.0% |
| !!! | TTAATTTGTAGAATTATATG+AGG | - | chr6.4:30891619-30891638 | MS.gene93432:CDS | 15.0% |
| !! | AGACATAAGAATAACAAAAT+TGG | + | chr6.4:30891596-30891615 | None:intergenic | 20.0% |
| !! | TTCAATTATTCATAACAAAC+AGG | + | chr6.4:30891079-30891098 | None:intergenic | 20.0% |
| !!! | TGTAATCTAAATTAGTCTTA+AGG | + | chr6.4:30891479-30891498 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTAAGACTGAAAC+AGG | + | chr6.4:30891432-30891451 | None:intergenic | 20.0% |
| ! | AAGAAGAATATTGAAATCCT+TGG | + | chr6.4:30890877-30890896 | None:intergenic | 25.0% |
| ! | ACATGCCATAAACAAAAAAT+AGG | + | chr6.4:30891561-30891580 | None:intergenic | 25.0% |
| ! | CTAATAAGACTAAAACAGAA+AGG | + | chr6.4:30891360-30891379 | None:intergenic | 25.0% |
| ! | TTGTAAAAAAAAACCACGTT+TGG | - | chr6.4:30891134-30891153 | MS.gene93432:intron | 25.0% |
| !! | ATGTTTTTCTATTTGTGGTT+AGG | - | chr6.4:30891307-30891326 | MS.gene93432:intron | 25.0% |
| !! | TCATTCTATTTGTGTTATTG+CGG | - | chr6.4:30890788-30890807 | MS.gene93432:intron | 25.0% |
| !!! | GATTCATGACTAATTTGTTT+TGG | - | chr6.4:30891398-30891417 | MS.gene93432:intron | 25.0% |
| !!! | TATGGCCTATTTTTTGTTTA+TGG | - | chr6.4:30891553-30891572 | MS.gene93432:intron | 25.0% |
| !!! | TTAGTTTTTCTTTGCAGTTT+TGG | - | chr6.4:30891281-30891300 | MS.gene93432:intron | 25.0% |
| !!! | TTCTGTTTTAGTCTTATTAG+AGG | - | chr6.4:30891360-30891379 | MS.gene93432:intron | 25.0% |
| AAAAAAAAACCACGTTTGGA+AGG | - | chr6.4:30891138-30891157 | MS.gene93432:intron | 30.0% | |
| ATAAACATACACAGCAACAA+TGG | + | chr6.4:30891536-30891555 | None:intergenic | 30.0% | |
| ATTACTCATTTGTCTTATGG+TGG | - | chr6.4:30890909-30890928 | MS.gene93432:intron | 30.0% | |
| ATTGTTGCTGTGTATGTTTA+TGG | - | chr6.4:30891535-30891554 | MS.gene93432:intron | 30.0% | |
| GCTATTACTCATTTGTCTTA+TGG | - | chr6.4:30890906-30890925 | MS.gene93432:intron | 30.0% | |
| !! | GGTTGATGTTTTTCTATTTG+TGG | - | chr6.4:30891302-30891321 | MS.gene93432:intron | 30.0% |
| !! | TTAGTCTTATTAGAGGAAGT+AGG | - | chr6.4:30891367-30891386 | MS.gene93432:intron | 30.0% |
| !!! | TTTTTAAGACTGAAACAGGA+AGG | + | chr6.4:30891428-30891447 | None:intergenic | 30.0% |
| AAACAAAGAAGAAACGAACC+TGG | + | chr6.4:30890749-30890768 | None:intergenic | 35.0% | |
| GCTAAAAACAGCAAAACAAC+AGG | + | chr6.4:30891184-30891203 | None:intergenic | 35.0% | |
| GTTCAAGAAGAAGAAACTCA+TGG | - | chr6.4:30891653-30891672 | MS.gene93432:CDS | 35.0% | |
| ! | AGAACAAATCGAGTTGTTGA+AGG | + | chr6.4:30890707-30890726 | None:intergenic | 35.0% |
| !!! | GTTGTTTTGCTGTTTTTAGC+TGG | - | chr6.4:30891184-30891203 | MS.gene93432:intron | 35.0% |
| !!! | TGCTGTTTTGGACATTGTTT+TGG | - | chr6.4:30891330-30891349 | MS.gene93432:intron | 35.0% |
| !!! | TTGCTGTTTTTAGCTGGTTT+AGG | - | chr6.4:30891190-30891209 | MS.gene93432:intron | 35.0% |
| AAGAGAGAGAATGAGATCTG+AGG | + | chr6.4:30890659-30890678 | None:intergenic | 40.0% | |
| CTTATGGTGGTCAATTCTCT+AGG | - | chr6.4:30890922-30890941 | MS.gene93432:intron | 40.0% | |
| TAGACAAGCATCAAACAGTG+TGG | - | chr6.4:30891679-30891698 | MS.gene93432:CDS | 40.0% | |
| TCAGGTTTCAGCTCTTGTTA+GGG | - | chr6.4:30890833-30890852 | MS.gene93432:intron | 40.0% | |
| ! | CTTCTTCTTCATCGTTTTCC+AGG | - | chr6.4:30890728-30890747 | MS.gene93432:intron | 40.0% |
| !! | TAGGGATAGTCTTGTGAGAA+GGG | - | chr6.4:30890851-30890870 | MS.gene93432:intron | 40.0% |
| !! | TTAGGGATAGTCTTGTGAGA+AGG | - | chr6.4:30890850-30890869 | MS.gene93432:intron | 40.0% |
| !!! | TTTGTGGTTAGGTGCTGTTT+TGG | - | chr6.4:30891318-30891337 | MS.gene93432:intron | 40.0% |
| CGGATCAACTTCTTACAGAC+CGG | - | chr6.4:30890808-30890827 | MS.gene93432:intron | 45.0% | |
| CTAACAAGAGCTGAAACCTG+AGG | + | chr6.4:30890834-30890853 | None:intergenic | 45.0% | |
| CTCAGGTTTCAGCTCTTGTT+AGG | - | chr6.4:30890832-30890851 | MS.gene93432:intron | 45.0% | |
| ! | TTAGCTGGTTTAGGTGCTGT+TGG | - | chr6.4:30891199-30891218 | MS.gene93432:intron | 45.0% |
| AGACTGTCACCTTCCAAACG+TGG | + | chr6.4:30891150-30891169 | None:intergenic | 50.0% | |
| !! | TAGTCTTGTGAGAAGGGCCA+AGG | - | chr6.4:30890857-30890876 | MS.gene93432:intron | 50.0% |
| ACTTCTTACAGACCGGCCTC+AGG | - | chr6.4:30890815-30890834 | MS.gene93432:intron | 55.0% | |
| CAAGAGCTGAAACCTGAGGC+CGG | + | chr6.4:30890830-30890849 | None:intergenic | 55.0% | |
| AGTCTCAGTTGCGGCAGCAG+GGG | - | chr6.4:30891239-30891258 | MS.gene93432:intron | 60.0% | |
| CAGTCTCAGTTGCGGCAGCA+GGG | - | chr6.4:30891238-30891257 | MS.gene93432:intron | 60.0% | |
| TGTCCGGCCAGTCTCAGTTG+CGG | - | chr6.4:30891230-30891249 | MS.gene93432:intron | 60.0% | |
| ! | GCTGTTGGTGCCTTGCTGTC+CGG | - | chr6.4:30891214-30891233 | MS.gene93432:intron | 60.0% |
| CCAGTCTCAGTTGCGGCAGC+AGG | - | chr6.4:30891237-30891256 | MS.gene93432:intron | 65.0% | |
| CCTGCTGCCGCAACTGAGAC+TGG | + | chr6.4:30891240-30891259 | None:intergenic | 65.0% | |
| CGGCAGCAGGGGTCTAGTGA+GGG | - | chr6.4:30891250-30891269 | MS.gene93432:intron | 65.0% | |
| CTGCCGCAACTGAGACTGGC+CGG | + | chr6.4:30891236-30891255 | None:intergenic | 65.0% | |
| ! | CTGAGACTGGCCGGACAGCA+AGG | + | chr6.4:30891227-30891246 | None:intergenic | 65.0% |
| GCGGCAGCAGGGGTCTAGTG+AGG | - | chr6.4:30891249-30891268 | MS.gene93432:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 30890636 | 30891710 | 30890636 | ID=MS.gene93432 |
| chr6.4 | mRNA | 30890636 | 30891710 | 30890636 | ID=MS.gene93432.t1;Parent=MS.gene93432 |
| chr6.4 | exon | 30891597 | 30891710 | 30891597 | ID=MS.gene93432.t1.exon1;Parent=MS.gene93432.t1 |
| chr6.4 | CDS | 30891597 | 30891710 | 30891597 | ID=cds.MS.gene93432.t1;Parent=MS.gene93432.t1 |
| chr6.4 | exon | 30891403 | 30891509 | 30891403 | ID=MS.gene93432.t1.exon2;Parent=MS.gene93432.t1 |
| chr6.4 | CDS | 30891403 | 30891509 | 30891403 | ID=cds.MS.gene93432.t1;Parent=MS.gene93432.t1 |
| chr6.4 | exon | 30891086 | 30891134 | 30891086 | ID=MS.gene93432.t1.exon3;Parent=MS.gene93432.t1 |
| chr6.4 | CDS | 30891086 | 30891134 | 30891086 | ID=cds.MS.gene93432.t1;Parent=MS.gene93432.t1 |
| chr6.4 | exon | 30890636 | 30890716 | 30890636 | ID=MS.gene93432.t1.exon4;Parent=MS.gene93432.t1 |
| chr6.4 | CDS | 30890636 | 30890716 | 30890636 | ID=cds.MS.gene93432.t1;Parent=MS.gene93432.t1 |
| Gene Sequence |
| Protein sequence |
