Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93845.t1 | XP_024626105.1 | 84 | 181 | 29 | 0 | 1 | 181 | 1 | 181 | 7.40E-68 | 266.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93845.t1 | A0A396I567 | 61.0 | 187 | 68 | 2 | 1 | 182 | 1 | 187 | 7.8e-43 | 183.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051932 | MS.gene93845 | 0.902378 | 1.03E-78 | -1.69E-46 |
| MS.gene053491 | MS.gene93845 | 0.955972 | 8.80E-114 | -1.69E-46 |
| MS.gene06044 | MS.gene93845 | 0.918226 | 2.02E-86 | -1.69E-46 |
| MS.gene061245 | MS.gene93845 | 0.881778 | 1.81E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93845.t1 | MTR_8g007240 | 32.738 | 168 | 63 | 7 | 17 | 182 | 49 | 168 | 2.47e-14 | 68.2 |
| MS.gene93845.t1 | MTR_0903s0010 | 29.787 | 188 | 72 | 7 | 14 | 192 | 42 | 178 | 1.17e-13 | 66.6 |
| MS.gene93845.t1 | MTR_3g102970 | 31.548 | 168 | 65 | 7 | 17 | 182 | 49 | 168 | 1.18e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGAAATTGGAATTGAATT+TGG | 0.155038 | 1.2:+78113075 | MS.gene93845:CDS |
| AAAATCCAAAGAGGAAATTT+TGG | 0.165439 | 1.2:+78112708 | MS.gene93845:CDS |
| TCCGATTACTTAAAGCAATT+TGG | 0.183536 | 1.2:+78113039 | MS.gene93845:CDS |
| CATCTCCAAAATTTCCTCTT+TGG | 0.214318 | 1.2:-78112713 | None:intergenic |
| TTTGGAGATGTCGAAACTTT+TGG | 0.226089 | 1.2:+78112726 | MS.gene93845:CDS |
| ACCATTGTAATTTGTTCATA+TGG | 0.234426 | 1.2:+78112839 | MS.gene93845:CDS |
| GCCAAATTGCTTTAAGTAAT+CGG | 0.251128 | 1.2:-78113040 | None:intergenic |
| AATTGAATTTGGAAAAGAAT+TGG | 0.290392 | 1.2:+78113086 | MS.gene93845:CDS |
| ATTGAATTTGGAAAAGAATT+GGG | 0.336217 | 1.2:+78113087 | MS.gene93845:CDS |
| TTTGGTAAATTTCCTCCAAT+TGG | 0.356380 | 1.2:-78112638 | None:intergenic |
| AAAGAGATTGGGAAGGAAAT+TGG | 0.360942 | 1.2:+78113063 | MS.gene93845:CDS |
| AAGCAATTTGGCAAAGAGAT+TGG | 0.365860 | 1.2:+78113051 | MS.gene93845:CDS |
| CCATTGTAATTTGTTCATAT+GGG | 0.394671 | 1.2:+78112840 | MS.gene93845:CDS |
| CCCAACTACAGCCTTCTTTG+AGG | 0.420158 | 1.2:-78112995 | None:intergenic |
| AGCAATTTGGCAAAGAGATT+GGG | 0.422113 | 1.2:+78113052 | MS.gene93845:CDS |
| TGTTTGCACTTGTGTTGTCA+TGG | 0.425538 | 1.2:+78113199 | MS.gene93845:CDS |
| TCAGCTGTTGGGTATCCAAT+TGG | 0.433222 | 1.2:+78112623 | MS.gene93845:CDS |
| TTAAGCATTGCTCTAACTCT+AGG | 0.453670 | 1.2:-78112880 | None:intergenic |
| TACTTGCGTGAAAGTGTCTC+TGG | 0.472823 | 1.2:+78113117 | MS.gene93845:CDS |
| GGTAGCAAAGCTTCAGCTGT+TGG | 0.480338 | 1.2:+78112611 | MS.gene93845:CDS |
| GTAGCAAAGCTTCAGCTGTT+GGG | 0.484076 | 1.2:+78112612 | MS.gene93845:CDS |
| TGAGGACACATCATTATTTG+AGG | 0.495657 | 1.2:-78112977 | None:intergenic |
| GTTTGCACTTGTGTTGTCAT+GGG | 0.499878 | 1.2:+78113200 | MS.gene93845:CDS |
| TCCTCAAAGAAGGCTGTAGT+TGG | 0.512316 | 1.2:+78112994 | MS.gene93845:CDS |
| TAATGATGTGTCCTCAAAGA+AGG | 0.517374 | 1.2:+78112984 | MS.gene93845:CDS |
| TTCCAGTACCTTTCAAAATG+TGG | 0.526356 | 1.2:+78112954 | MS.gene93845:CDS |
| GTAATCAGATGATGAAATTG+TGG | 0.528968 | 1.2:+78112669 | MS.gene93845:CDS |
| ATCTGATTACAACCACACTT+TGG | 0.542708 | 1.2:-78112656 | None:intergenic |
| GCTGTTGGGTATCCAATTGG+AGG | 0.550598 | 1.2:+78112626 | MS.gene93845:CDS |
| CCTCAAAGAAGGCTGTAGTT+GGG | 0.553172 | 1.2:+78112995 | MS.gene93845:CDS |
| TAATCAGATGATGAAATTGT+GGG | 0.555715 | 1.2:+78112670 | MS.gene93845:CDS |
| ATTTGGAAAAGAATTGGGCA+AGG | 0.572988 | 1.2:+78113092 | MS.gene93845:CDS |
| GGAGGAAATTTACCAAAGTG+TGG | 0.573900 | 1.2:+78112644 | MS.gene93845:CDS |
| ACAACACAAGTGCAAACAGT+AGG | 0.614937 | 1.2:-78113194 | None:intergenic |
| CCCATATGAACAAATTACAA+TGG | 0.636074 | 1.2:-78112840 | None:intergenic |
| ATTGCTGCAAAGAACAGTGA+AGG | 0.636453 | 1.2:+78112907 | MS.gene93845:CDS |
| ATACAGATGAAAATCCAAAG+AGG | 0.650224 | 1.2:+78112699 | MS.gene93845:CDS |
| ATTTGGCAAAGAGATTGGGA+AGG | 0.653755 | 1.2:+78113056 | MS.gene93845:CDS |
| AAGAACAGTGAAGGTGATGG+TGG | 0.677445 | 1.2:+78112916 | MS.gene93845:CDS |
| GCAAAGAACAGTGAAGGTGA+TGG | 0.689878 | 1.2:+78112913 | MS.gene93845:CDS |
| GTTAGGGTTCCTGAAATGGG+GGG | 0.697416 | 1.2:+78112590 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAATTTGGAAAAGAAT+TGG | + | chr1.2:78113086-78113105 | MS.gene93845:CDS | 20.0% |
| !! | ATTGAATTTGGAAAAGAATT+GGG | + | chr1.2:78113087-78113106 | MS.gene93845:CDS | 20.0% |
| !!! | AAATCTGATTTGTATGTATT+TGG | + | chr1.2:78112764-78112783 | MS.gene93845:intron | 20.0% |
| ! | AAGGAAATTGGAATTGAATT+TGG | + | chr1.2:78113075-78113094 | MS.gene93845:CDS | 25.0% |
| ! | TAATCAGATGATGAAATTGT+GGG | + | chr1.2:78112670-78112689 | MS.gene93845:CDS | 25.0% |
| !! | AAAATCCAAAGAGGAAATTT+TGG | + | chr1.2:78112708-78112727 | MS.gene93845:CDS | 25.0% |
| !!! | ACCATTGTAATTTGTTCATA+TGG | + | chr1.2:78112839-78112858 | MS.gene93845:CDS | 25.0% |
| !!! | CCATTGTAATTTGTTCATAT+GGG | + | chr1.2:78112840-78112859 | MS.gene93845:CDS | 25.0% |
| ATACAGATGAAAATCCAAAG+AGG | + | chr1.2:78112699-78112718 | MS.gene93845:CDS | 30.0% | |
| CCCATATGAACAAATTACAA+TGG | - | chr1.2:78112843-78112862 | None:intergenic | 30.0% | |
| GTAATCAGATGATGAAATTG+TGG | + | chr1.2:78112669-78112688 | MS.gene93845:CDS | 30.0% | |
| TCCGATTACTTAAAGCAATT+TGG | + | chr1.2:78113039-78113058 | MS.gene93845:CDS | 30.0% | |
| TTTGGTAAATTTCCTCCAAT+TGG | - | chr1.2:78112641-78112660 | None:intergenic | 30.0% | |
| ! | GCCAAATTGCTTTAAGTAAT+CGG | - | chr1.2:78113043-78113062 | None:intergenic | 30.0% |
| AAAGAGATTGGGAAGGAAAT+TGG | + | chr1.2:78113063-78113082 | MS.gene93845:CDS | 35.0% | |
| AAGCAATTTGGCAAAGAGAT+TGG | + | chr1.2:78113051-78113070 | MS.gene93845:CDS | 35.0% | |
| AGCAATTTGGCAAAGAGATT+GGG | + | chr1.2:78113052-78113071 | MS.gene93845:CDS | 35.0% | |
| ATCTGATTACAACCACACTT+TGG | - | chr1.2:78112659-78112678 | None:intergenic | 35.0% | |
| ATTTGGAAAAGAATTGGGCA+AGG | + | chr1.2:78113092-78113111 | MS.gene93845:CDS | 35.0% | |
| CATCTCCAAAATTTCCTCTT+TGG | - | chr1.2:78112716-78112735 | None:intergenic | 35.0% | |
| TAATGATGTGTCCTCAAAGA+AGG | + | chr1.2:78112984-78113003 | MS.gene93845:CDS | 35.0% | |
| TGAGGACACATCATTATTTG+AGG | - | chr1.2:78112980-78112999 | None:intergenic | 35.0% | |
| TTCCAGTACCTTTCAAAATG+TGG | + | chr1.2:78112954-78112973 | MS.gene93845:CDS | 35.0% | |
| ! | TTAAGCATTGCTCTAACTCT+AGG | - | chr1.2:78112883-78112902 | None:intergenic | 35.0% |
| ! | TTGGAGATGTCGAAACTTTT+GGG | + | chr1.2:78112727-78112746 | MS.gene93845:CDS | 35.0% |
| ! | TTTGGAGATGTCGAAACTTT+TGG | + | chr1.2:78112726-78112745 | MS.gene93845:CDS | 35.0% |
| !! | ATTTGAGGCCACATTTTGAA+AGG | - | chr1.2:78112965-78112984 | None:intergenic | 35.0% |
| ACAACACAAGTGCAAACAGT+AGG | - | chr1.2:78113197-78113216 | None:intergenic | 40.0% | |
| ATTGCTGCAAAGAACAGTGA+AGG | + | chr1.2:78112907-78112926 | MS.gene93845:CDS | 40.0% | |
| ATTTGGCAAAGAGATTGGGA+AGG | + | chr1.2:78113056-78113075 | MS.gene93845:CDS | 40.0% | |
| GGAGGAAATTTACCAAAGTG+TGG | + | chr1.2:78112644-78112663 | MS.gene93845:CDS | 40.0% | |
| GTTTGCACTTGTGTTGTCAT+GGG | + | chr1.2:78113200-78113219 | MS.gene93845:CDS | 40.0% | |
| TGTTTGCACTTGTGTTGTCA+TGG | + | chr1.2:78113199-78113218 | MS.gene93845:CDS | 40.0% | |
| !!! | GATGGTGGTTTTAGCAACAT+TGG | + | chr1.2:78112931-78112950 | MS.gene93845:CDS | 40.0% |
| CCTCAAAGAAGGCTGTAGTT+GGG | + | chr1.2:78112995-78113014 | MS.gene93845:CDS | 45.0% | |
| GCAAAGAACAGTGAAGGTGA+TGG | + | chr1.2:78112913-78112932 | MS.gene93845:CDS | 45.0% | |
| GTAGCAAAGCTTCAGCTGTT+GGG | + | chr1.2:78112612-78112631 | MS.gene93845:CDS | 45.0% | |
| TCAGCTGTTGGGTATCCAAT+TGG | + | chr1.2:78112623-78112642 | MS.gene93845:CDS | 45.0% | |
| TCCTCAAAGAAGGCTGTAGT+TGG | + | chr1.2:78112994-78113013 | MS.gene93845:CDS | 45.0% | |
| ! | AAGAACAGTGAAGGTGATGG+TGG | + | chr1.2:78112916-78112935 | MS.gene93845:CDS | 45.0% |
| ! | GAGATGTCGAAACTTTTGGG+AGG | + | chr1.2:78112730-78112749 | MS.gene93845:CDS | 45.0% |
| !! | GGCCACATTTTGAAAGGTAC+TGG | - | chr1.2:78112959-78112978 | None:intergenic | 45.0% |
| !! | TACTTGCGTGAAAGTGTCTC+TGG | + | chr1.2:78113117-78113136 | MS.gene93845:CDS | 45.0% |
| CCCAACTACAGCCTTCTTTG+AGG | - | chr1.2:78112998-78113017 | None:intergenic | 50.0% | |
| GCTGTTGGGTATCCAATTGG+AGG | + | chr1.2:78112626-78112645 | MS.gene93845:CDS | 50.0% | |
| GGTAGCAAAGCTTCAGCTGT+TGG | + | chr1.2:78112611-78112630 | MS.gene93845:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 78112605 | 78113290 | 78112605 | ID=MS.gene93845 |
| chr1.2 | mRNA | 78112605 | 78113290 | 78112605 | ID=MS.gene93845.t1;Parent=MS.gene93845 |
| chr1.2 | exon | 78112605 | 78112751 | 78112605 | ID=MS.gene93845.t1.exon1;Parent=MS.gene93845.t1 |
| chr1.2 | CDS | 78112605 | 78112751 | 78112605 | ID=cds.MS.gene93845.t1;Parent=MS.gene93845.t1 |
| chr1.2 | exon | 78112832 | 78113290 | 78112832 | ID=MS.gene93845.t1.exon2;Parent=MS.gene93845.t1 |
| chr1.2 | CDS | 78112832 | 78113290 | 78112832 | ID=cds.MS.gene93845.t1;Parent=MS.gene93845.t1 |
| Gene Sequence |
| Protein sequence |
