Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94034.t1 | RHN52392.1 | 100 | 322 | 0 | 0 | 1 | 322 | 1 | 322 | 9.10E-185 | 656 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94034.t1 | A0A2P1GIY9 | 60.6 | 320 | 122 | 3 | 5 | 322 | 6 | 323 | 1.3e-110 | 401.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94034.t1 | A0A396HGB9 | 100.0 | 322 | 0 | 0 | 1 | 322 | 1 | 322 | 6.6e-185 | 656.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05081 | MS.gene94034 | 0.83271 | 8.09E-56 | -1.69E-46 |
| MS.gene052870 | MS.gene94034 | 0.821461 | 3.99E-53 | -1.69E-46 |
| MS.gene052872 | MS.gene94034 | 0.816083 | 6.64E-52 | -1.69E-46 |
| MS.gene05298 | MS.gene94034 | 0.809427 | 1.90E-50 | -1.69E-46 |
| MS.gene05494 | MS.gene94034 | 0.819776 | 9.72E-53 | -1.69E-46 |
| MS.gene055206 | MS.gene94034 | 0.830074 | 3.60E-55 | -1.69E-46 |
| MS.gene057289 | MS.gene94034 | 0.806242 | 9.05E-50 | -1.69E-46 |
| MS.gene059129 | MS.gene94034 | 0.812989 | 3.21E-51 | -1.69E-46 |
| MS.gene060717 | MS.gene94034 | 0.803645 | 3.16E-49 | -1.69E-46 |
| MS.gene061231 | MS.gene94034 | 0.806604 | 7.59E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94034.t1 | MTR_4g072350 | 65.421 | 321 | 110 | 1 | 1 | 321 | 1 | 320 | 4.26e-159 | 447 |
| MS.gene94034.t1 | MTR_4g072320 | 65.528 | 322 | 104 | 3 | 1 | 322 | 1 | 315 | 3.68e-157 | 442 |
| MS.gene94034.t1 | MTR_4g072360 | 64.174 | 321 | 114 | 1 | 1 | 321 | 1 | 320 | 1.57e-154 | 436 |
| MS.gene94034.t1 | MTR_6g073110 | 58.307 | 319 | 132 | 1 | 1 | 319 | 1 | 318 | 6.84e-138 | 393 |
| MS.gene94034.t1 | MTR_0374s0050 | 49.538 | 325 | 150 | 4 | 1 | 321 | 1 | 315 | 4.77e-107 | 315 |
| MS.gene94034.t1 | MTR_3g083130 | 48.765 | 324 | 147 | 7 | 2 | 319 | 3 | 313 | 8.64e-98 | 291 |
| MS.gene94034.t1 | MTR_5g097910 | 49.677 | 310 | 137 | 8 | 1 | 301 | 1 | 300 | 9.40e-88 | 266 |
| MS.gene94034.t1 | MTR_5g097940 | 49.677 | 310 | 137 | 8 | 1 | 301 | 1 | 300 | 9.40e-88 | 266 |
| MS.gene94034.t1 | MTR_5g097900 | 49.677 | 310 | 137 | 8 | 1 | 301 | 1 | 300 | 1.06e-87 | 265 |
| MS.gene94034.t1 | MTR_8g070095 | 44.654 | 318 | 162 | 8 | 11 | 322 | 10 | 319 | 1.54e-87 | 265 |
| MS.gene94034.t1 | MTR_4g036845 | 43.849 | 317 | 170 | 6 | 8 | 322 | 5 | 315 | 1.96e-86 | 262 |
| MS.gene94034.t1 | MTR_8g070115 | 43.750 | 320 | 172 | 4 | 4 | 322 | 3 | 315 | 5.03e-84 | 256 |
| MS.gene94034.t1 | MTR_4g021350 | 43.770 | 313 | 166 | 5 | 11 | 322 | 10 | 313 | 4.33e-76 | 236 |
| MS.gene94034.t1 | MTR_6g072710 | 95.575 | 113 | 5 | 0 | 191 | 303 | 1 | 113 | 8.48e-75 | 225 |
| MS.gene94034.t1 | MTR_7g063580 | 41.214 | 313 | 175 | 5 | 11 | 322 | 50 | 354 | 1.28e-69 | 221 |
| MS.gene94034.t1 | MTR_4g021350 | 44.561 | 285 | 149 | 4 | 11 | 295 | 10 | 285 | 2.27e-69 | 218 |
| MS.gene94034.t1 | MTR_7g070500 | 38.141 | 312 | 180 | 6 | 11 | 319 | 17 | 318 | 2.27e-68 | 216 |
| MS.gene94034.t1 | MTR_4g021450 | 42.173 | 313 | 160 | 6 | 11 | 321 | 11 | 304 | 2.60e-68 | 216 |
| MS.gene94034.t1 | MTR_4g021410 | 41.158 | 311 | 162 | 6 | 11 | 319 | 11 | 302 | 1.25e-66 | 211 |
| MS.gene94034.t1 | MTR_7g070500 | 38.811 | 286 | 165 | 4 | 11 | 296 | 17 | 292 | 6.98e-64 | 204 |
| MS.gene94034.t1 | MTR_4g021410 | 42.294 | 279 | 143 | 5 | 43 | 319 | 3 | 265 | 3.44e-62 | 199 |
| MS.gene94034.t1 | MTR_4g072060 | 39.667 | 300 | 164 | 7 | 9 | 299 | 3 | 294 | 3.56e-59 | 192 |
| MS.gene94034.t1 | MTR_6g072650 | 57.237 | 152 | 43 | 1 | 170 | 321 | 7 | 136 | 4.58e-54 | 173 |
| MS.gene94034.t1 | MTR_4g021450 | 42.798 | 243 | 122 | 4 | 81 | 321 | 1 | 228 | 1.61e-51 | 170 |
| MS.gene94034.t1 | MTR_4g021410 | 41.494 | 241 | 124 | 4 | 81 | 319 | 1 | 226 | 9.90e-49 | 163 |
| MS.gene94034.t1 | MTR_4g021450 | 45.000 | 180 | 94 | 2 | 143 | 321 | 10 | 185 | 1.46e-47 | 158 |
| MS.gene94034.t1 | MTR_6g072705 | 63.265 | 49 | 17 | 1 | 105 | 153 | 38 | 85 | 5.34e-15 | 69.7 |
| MS.gene94034.t1 | MTR_7g114990 | 25.455 | 330 | 173 | 13 | 6 | 290 | 13 | 314 | 1.08e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94034.t1 | AT1G59960 | 46.037 | 328 | 169 | 4 | 1 | 322 | 1 | 326 | 4.65e-107 | 315 |
| MS.gene94034.t1 | AT1G59950 | 48.065 | 310 | 157 | 3 | 15 | 322 | 13 | 320 | 1.60e-98 | 293 |
| MS.gene94034.t1 | AT2G37770 | 46.106 | 321 | 165 | 4 | 3 | 322 | 2 | 315 | 5.55e-92 | 276 |
| MS.gene94034.t1 | AT2G37790 | 45.192 | 312 | 164 | 3 | 11 | 322 | 10 | 314 | 8.67e-92 | 276 |
| MS.gene94034.t1 | AT2G37760 | 45.367 | 313 | 159 | 4 | 11 | 322 | 10 | 311 | 1.91e-86 | 262 |
| MS.gene94034.t1 | AT2G37760 | 45.367 | 313 | 159 | 4 | 11 | 322 | 10 | 311 | 1.91e-86 | 262 |
| MS.gene94034.t1 | AT5G62420 | 41.587 | 315 | 175 | 6 | 11 | 322 | 8 | 316 | 3.46e-86 | 262 |
| MS.gene94034.t1 | AT3G53880 | 44.728 | 313 | 165 | 4 | 11 | 322 | 10 | 315 | 8.24e-85 | 258 |
| MS.gene94034.t1 | AT2G37760 | 46.207 | 290 | 145 | 3 | 11 | 300 | 10 | 288 | 1.40e-82 | 252 |
| MS.gene94034.t1 | AT2G37760 | 45.993 | 287 | 144 | 3 | 11 | 297 | 10 | 285 | 3.00e-81 | 248 |
| MS.gene94034.t1 | AT2G37760 | 45.993 | 287 | 144 | 3 | 11 | 297 | 10 | 285 | 3.00e-81 | 248 |
| MS.gene94034.t1 | AT3G53880 | 44.561 | 285 | 151 | 3 | 11 | 295 | 10 | 287 | 1.51e-77 | 239 |
| MS.gene94034.t1 | AT5G01670 | 37.188 | 320 | 188 | 6 | 3 | 319 | 10 | 319 | 1.14e-74 | 233 |
| MS.gene94034.t1 | AT2G37760 | 45.802 | 262 | 137 | 3 | 62 | 322 | 8 | 265 | 6.83e-70 | 218 |
| MS.gene94034.t1 | AT2G37760 | 45.802 | 262 | 137 | 3 | 62 | 322 | 8 | 265 | 6.83e-70 | 218 |
| MS.gene94034.t1 | AT5G01670 | 34.294 | 347 | 188 | 7 | 3 | 319 | 10 | 346 | 1.21e-68 | 218 |
| MS.gene94034.t1 | AT2G21250 | 37.248 | 298 | 172 | 6 | 8 | 296 | 2 | 293 | 8.34e-64 | 204 |
| MS.gene94034.t1 | AT2G21260 | 37.037 | 297 | 172 | 6 | 8 | 295 | 2 | 292 | 2.32e-63 | 203 |
| MS.gene94034.t1 | AT2G37770 | 49.524 | 210 | 99 | 3 | 3 | 212 | 2 | 204 | 4.43e-62 | 199 |
| MS.gene94034.t1 | AT2G21250 | 36.555 | 238 | 136 | 6 | 8 | 236 | 2 | 233 | 8.28e-46 | 155 |
| MS.gene94034.t1 | AT2G21260 | 34.101 | 217 | 132 | 4 | 92 | 299 | 12 | 226 | 4.14e-38 | 135 |
| MS.gene94034.t1 | AT5G53580 | 24.172 | 302 | 170 | 10 | 21 | 286 | 64 | 342 | 1.65e-14 | 73.9 |
Find 78 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTGCACTTGACCCTATT+TGG | 0.175394 | 6.4:+49718810 | None:intergenic |
| TTCGATTCTGCTTCTGTCTA+TGG | 0.228641 | 6.4:-49727046 | MS.gene94034:CDS |
| CTTAGATGGGTGTACCAAAT+AGG | 0.259918 | 6.4:-49718824 | MS.gene94034:CDS |
| TCTATTGGCATATGCAATTA+TGG | 0.277871 | 6.4:-49725496 | MS.gene94034:CDS |
| TTACTTCCCTTTGATATAGA+AGG | 0.286868 | 6.4:-49725565 | MS.gene94034:CDS |
| AAAGGGAAGTAAATCTTCTT+TGG | 0.295146 | 6.4:+49725576 | None:intergenic |
| GTCAGCTCTTGGAGCTTATA+AGG | 0.323302 | 6.4:-49719096 | MS.gene94034:CDS |
| ATTTGCAGGTTGCACTTAGA+TGG | 0.331893 | 6.4:-49718838 | MS.gene94034:intron |
| TACAATGTTGTTTAGAAAGC+TGG | 0.334762 | 6.4:-49725677 | MS.gene94034:intron |
| CCTGAGTTTAACAACACTTC+TGG | 0.338648 | 6.4:+49727175 | None:intergenic |
| TGGATCTTTATCTGATTCAT+TGG | 0.339555 | 6.4:-49725642 | MS.gene94034:CDS |
| GACATGTGGCTATCTGAAAA+TGG | 0.360055 | 6.4:-49718671 | MS.gene94034:CDS |
| ATGGAAGAGTGTTATAAGTT+AGG | 0.361957 | 6.4:-49725529 | MS.gene94034:CDS |
| CATCACCCCTGCAGTGAATC+AGG | 0.364569 | 6.4:-49725437 | MS.gene94034:intron |
| TATAAGGTAACTTGGGGTTC+AGG | 0.370516 | 6.4:-49719080 | MS.gene94034:CDS |
| ACTCAAAATCAAATATTTCA+AGG | 0.375736 | 6.4:+49718753 | None:intergenic |
| GCAAGACCTTAGAAGAACTT+TGG | 0.387987 | 6.4:-49718643 | MS.gene94034:CDS |
| AGATCATGTCTCAGTCTTAC+TGG | 0.388003 | 6.4:+49725619 | None:intergenic |
| CAAGACCTTAGAAGAACTTT+GGG | 0.404358 | 6.4:-49718642 | MS.gene94034:CDS |
| GTTAGTGCCTGGTCAGCTCT+TGG | 0.409617 | 6.4:-49719107 | MS.gene94034:CDS |
| TCCATCCCAAAGTTCTTCTA+AGG | 0.411991 | 6.4:+49718637 | None:intergenic |
| GAAGCAAGAACATCATTTGA+TGG | 0.416424 | 6.4:+49727106 | None:intergenic |
| TTAGGCTTAGCAAAGTCTAT+TGG | 0.418231 | 6.4:-49725511 | MS.gene94034:CDS |
| TTTGTTGATGCAATCAAAGT+TGG | 0.422988 | 6.4:-49727079 | MS.gene94034:CDS |
| TCTTCTTTGGTAAATATGAC+AGG | 0.431223 | 6.4:+49725589 | None:intergenic |
| GGACACAAAATGCCAGTCAT+AGG | 0.432511 | 6.4:-49727154 | MS.gene94034:CDS |
| ACATGTCTCCTAAGTATTGC+CGG | 0.439549 | 6.4:+49718687 | None:intergenic |
| TGCATCAGTGTTCCATGGCT+TGG | 0.441013 | 6.4:+49726940 | None:intergenic |
| AGGTGGAGATGAATCCTTCT+TGG | 0.442298 | 6.4:-49719175 | MS.gene94034:CDS |
| TGGAGCTTATAAGGTAACTT+GGG | 0.443096 | 6.4:-49719087 | MS.gene94034:CDS |
| TTTAATGTGGTCTTAAGAGC+TGG | 0.453099 | 6.4:+49726899 | None:intergenic |
| TTAGATGGGTGTACCAAATA+GGG | 0.456759 | 6.4:-49718823 | MS.gene94034:CDS |
| CCATGTCCCTTCTATATCAA+AGG | 0.457170 | 6.4:+49725558 | None:intergenic |
| TTTGCAGGTTGCACTTAGAT+GGG | 0.473093 | 6.4:-49718837 | MS.gene94034:intron |
| TTGGAGCTTATAAGGTAACT+TGG | 0.473391 | 6.4:-49719088 | MS.gene94034:CDS |
| CCACAGCGCCGGCAATACTT+AGG | 0.473446 | 6.4:-49718695 | MS.gene94034:CDS |
| CTTGGCAGCAAGGGAAATTG+AGG | 0.483207 | 6.4:-49719157 | MS.gene94034:CDS |
| AAGACATAGCAGCTGCTAAA+GGG | 0.483325 | 6.4:-49719031 | MS.gene94034:CDS |
| CAAGACATAGCAGCTGCTAA+AGG | 0.487179 | 6.4:-49719032 | MS.gene94034:CDS |
| AATTTCCCTTGCTGCCAAGA+AGG | 0.487455 | 6.4:+49719161 | None:intergenic |
| CATGTCCCTTCTATATCAAA+GGG | 0.492063 | 6.4:+49725559 | None:intergenic |
| ATAAGCTCCAAGAGCTGACC+AGG | 0.495471 | 6.4:+49719100 | None:intergenic |
| AGACAGAAGCAGAATCGAAA+TGG | 0.496929 | 6.4:+49727050 | None:intergenic |
| CTGATTCACTGCAGGGGTGA+TGG | 0.501160 | 6.4:+49725438 | None:intergenic |
| TACTTCCCTTTGATATAGAA+GGG | 0.517381 | 6.4:-49725564 | MS.gene94034:CDS |
| TATGGAACAGAAGAAGCTAT+AGG | 0.520405 | 6.4:-49727028 | MS.gene94034:CDS |
| TTGGCAGCAAGGGAAATTGA+GGG | 0.524904 | 6.4:-49719156 | MS.gene94034:CDS |
| ATACATACCTGATTCACTGC+AGG | 0.528806 | 6.4:+49725430 | None:intergenic |
| AAAATGCCAGTCATAGGAAT+GGG | 0.533740 | 6.4:-49727148 | MS.gene94034:CDS |
| ACAATGTTGTTTAGAAAGCT+GGG | 0.535276 | 6.4:-49725676 | MS.gene94034:intron |
| TTGGGGTTCAGGCGCTGTCG+TGG | 0.539309 | 6.4:-49719069 | MS.gene94034:CDS |
| AAAGCTGGGAACGGAGTATG+TGG | 0.541166 | 6.4:-49725662 | MS.gene94034:CDS |
| CCTAAGTATTGCCGGCGCTG+TGG | 0.541530 | 6.4:+49718695 | None:intergenic |
| ATTCATAGCTTACTTTAATG+TGG | 0.541642 | 6.4:+49726886 | None:intergenic |
| CCTTTGATATAGAAGGGACA+TGG | 0.542358 | 6.4:-49725558 | MS.gene94034:CDS |
| AGGGAATTCATGTTAGTGCC+TGG | 0.542579 | 6.4:-49719118 | MS.gene94034:CDS |
| CAAAATGCCAGTCATAGGAA+TGG | 0.558036 | 6.4:-49727149 | MS.gene94034:CDS |
| ACCTTAGAAGAACTTTGGGA+TGG | 0.559848 | 6.4:-49718638 | MS.gene94034:CDS |
| CCAGAAGTGTTGTTAAACTC+AGG | 0.564492 | 6.4:-49727175 | MS.gene94034:CDS |
| ATGAATCCTTCTTGGCAGCA+AGG | 0.568216 | 6.4:-49719167 | MS.gene94034:CDS |
| CTTCTTTGGTAAATATGACA+GGG | 0.570915 | 6.4:+49725590 | None:intergenic |
| TACATACCTGATTCACTGCA+GGG | 0.572609 | 6.4:+49725431 | None:intergenic |
| CTAAAAGCTTTAACAAGGAG+AGG | 0.572880 | 6.4:-49718787 | MS.gene94034:CDS |
| CCAAATAGGGTCAAGTGCAA+TGG | 0.574378 | 6.4:-49718810 | MS.gene94034:CDS |
| GAAGTTCCCATTCCTATGAC+TGG | 0.576432 | 6.4:+49727142 | None:intergenic |
| TGAATCCTTCTTGGCAGCAA+GGG | 0.580141 | 6.4:-49719166 | MS.gene94034:CDS |
| TTAAGCAGATTCCACAGCGC+CGG | 0.581960 | 6.4:-49718706 | MS.gene94034:CDS |
| TAAAGGGAAGACTACAGCTC+AGG | 0.583393 | 6.4:-49719015 | MS.gene94034:intron |
| AGAAGGGACATGGAAAGCAA+TGG | 0.586366 | 6.4:-49725548 | MS.gene94034:CDS |
| GTTGTTTAGAAAGCTGGGAA+CGG | 0.591717 | 6.4:-49725671 | MS.gene94034:intron |
| TAATCTGCATCAGTGTTCCA+TGG | 0.604256 | 6.4:+49726935 | None:intergenic |
| GGAGCTTATAAGGTAACTTG+GGG | 0.607696 | 6.4:-49719086 | MS.gene94034:CDS |
| AGAAGCTATAGGCATGGCTG+TGG | 0.608547 | 6.4:-49727017 | MS.gene94034:CDS |
| AACAGAAGAAGCTATAGGCA+TGG | 0.636370 | 6.4:-49727023 | MS.gene94034:CDS |
| GTGGCAAAAGCTTTAGAACA+AGG | 0.642750 | 6.4:-49726998 | MS.gene94034:CDS |
| AATGGCTAAAAGCTTTAACA+AGG | 0.667938 | 6.4:-49718792 | MS.gene94034:CDS |
| GGCAATACTTAGGAGACATG+TGG | 0.679990 | 6.4:-49718685 | MS.gene94034:CDS |
| ACATACCTGATTCACTGCAG+GGG | 0.700439 | 6.4:+49725432 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 49718628 | 49727215 | 49718628 | ID=MS.gene94034 |
| chr6.4 | mRNA | 49718628 | 49727215 | 49718628 | ID=MS.gene94034.t1;Parent=MS.gene94034 |
| chr6.4 | exon | 49726899 | 49727215 | 49726899 | ID=MS.gene94034.t1.exon1;Parent=MS.gene94034.t1 |
| chr6.4 | CDS | 49726899 | 49727215 | 49726899 | ID=cds.MS.gene94034.t1;Parent=MS.gene94034.t1 |
| chr6.4 | exon | 49725438 | 49725684 | 49725438 | ID=MS.gene94034.t1.exon2;Parent=MS.gene94034.t1 |
| chr6.4 | CDS | 49725438 | 49725684 | 49725438 | ID=cds.MS.gene94034.t1;Parent=MS.gene94034.t1 |
| chr6.4 | exon | 49719016 | 49719195 | 49719016 | ID=MS.gene94034.t1.exon3;Parent=MS.gene94034.t1 |
| chr6.4 | CDS | 49719016 | 49719195 | 49719016 | ID=cds.MS.gene94034.t1;Parent=MS.gene94034.t1 |
| chr6.4 | exon | 49718628 | 49718852 | 49718628 | ID=MS.gene94034.t1.exon4;Parent=MS.gene94034.t1 |
| chr6.4 | CDS | 49718628 | 49718852 | 49718628 | ID=cds.MS.gene94034.t1;Parent=MS.gene94034.t1 |
| Gene Sequence |
| Protein sequence |
