AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94094

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94094.t1 MTR_5g059260 98.132 589 11 0 1 589 1 589 0.0 1210
MS.gene94094.t1 MTR_1g031590 55.307 537 214 6 26 559 33 546 0.0 602
MS.gene94094.t1 MTR_4g084570 57.143 497 195 4 68 559 64 547 0.0 600
MS.gene94094.t1 MTR_3g005340 55.227 507 209 5 72 569 57 554 0.0 568
MS.gene94094.t1 MTR_2g016470 39.073 453 238 10 139 561 172 616 4.36e-95 303
MS.gene94094.t1 MTR_5g032100 40.845 426 215 11 159 559 88 501 4.81e-94 297
MS.gene94094.t1 MTR_3g086570 37.762 429 247 9 139 559 131 547 1.30e-92 295
MS.gene94094.t1 MTR_4g100810 44.318 352 180 6 158 509 109 444 6.20e-92 293
MS.gene94094.t1 MTR_3g100310 39.671 426 220 10 159 559 102 515 2.87e-91 291
MS.gene94094.t1 MTR_5g064620 38.952 439 231 12 140 562 97 514 3.03e-90 288
MS.gene94094.t1 MTR_5g099010 43.562 365 188 6 160 523 122 469 9.06e-90 289
MS.gene94094.t1 MTR_3g115220 41.316 380 206 6 141 519 95 458 3.77e-89 287
MS.gene94094.t1 MTR_5g012770 44.101 356 164 9 158 509 125 449 4.85e-87 280
MS.gene94094.t1 MTR_3g099480 37.358 439 237 12 140 563 126 541 1.47e-85 276
MS.gene94094.t1 MTR_6g016015 39.036 415 228 10 158 564 116 513 2.18e-84 273
MS.gene94094.t1 MTR_4g110030 39.036 415 224 10 158 563 117 511 6.06e-83 269
MS.gene94094.t1 MTR_3g096020 39.089 417 221 10 157 559 189 586 3.28e-80 264
MS.gene94094.t1 MTR_2g059350 36.717 463 244 13 112 563 86 510 7.54e-80 261
MS.gene94094.t1 MTR_4g132590 37.389 452 240 9 140 559 52 492 2.04e-78 256
MS.gene94094.t1 MTR_4g082310 36.650 412 231 10 158 559 98 489 3.06e-78 256
MS.gene94094.t1 MTR_5g042200 36.893 412 230 10 158 559 86 477 3.51e-78 256
MS.gene94094.t1 MTR_4g132590 37.389 452 240 9 140 559 151 591 1.70e-77 257
MS.gene94094.t1 MTR_4g125460 37.089 426 232 12 146 559 77 478 3.78e-77 253
MS.gene94094.t1 MTR_5g081690 36.471 425 235 12 158 571 122 522 7.77e-77 253
MS.gene94094.t1 MTR_4g125460 37.089 426 232 12 146 559 216 617 7.24e-76 253
MS.gene94094.t1 MTR_4g019210 35.581 430 242 14 158 575 233 639 6.87e-73 246
MS.gene94094.t1 MTR_1g102030 35.597 427 217 15 158 559 89 482 2.91e-67 228
MS.gene94094.t1 MTR_3g086570 38.361 305 175 6 139 435 131 430 4.50e-67 225
MS.gene94094.t1 MTR_3g026650 32.494 437 246 8 139 559 153 556 2.28e-66 226
MS.gene94094.t1 MTR_4g023030 31.556 450 278 9 116 559 191 616 1.55e-61 214
MS.gene94094.t1 MTR_3g026650 33.333 357 195 6 139 479 153 482 5.56e-53 188
MS.gene94094.t1 MTR_4g010990 31.714 391 222 11 128 508 48 403 3.20e-48 174
MS.gene94094.t1 MTR_1g101990 35.000 300 166 10 267 559 1 278 2.55e-45 163
MS.gene94094.t1 MTR_4g010990 31.085 341 202 8 173 508 2 314 3.26e-41 153
MS.gene94094.t1 MTR_3g092590 32.895 304 188 6 158 452 91 387 4.32e-34 137
MS.gene94094.t1 MTR_7g017330 28.760 379 235 13 107 470 55 413 8.14e-34 137
MS.gene94094.t1 MTR_3g092590 32.787 305 187 7 158 452 91 387 9.80e-34 136
MS.gene94094.t1 MTR_3g026650 30.292 274 148 6 139 396 153 399 4.14e-29 120
MS.gene94094.t1 MTR_1g007390 31.270 307 187 8 158 448 91 389 6.26e-28 119
MS.gene94094.t1 MTR_4g008200 27.299 348 203 9 158 500 36 338 3.29e-26 110
MS.gene94094.t1 MTR_4g008200 27.299 348 203 9 158 500 87 389 7.54e-26 110
MS.gene94094.t1 MTR_2g038320 26.596 376 221 11 132 502 70 395 5.85e-25 108
MS.gene94094.t1 MTR_7g064210 33.500 200 106 4 278 466 27 210 1.23e-23 103
MS.gene94094.t1 MTR_1g101985 53.333 75 35 0 158 232 89 163 1.74e-18 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94094.t1 AT1G38131 69.231 494 145 2 72 560 67 558 0.0 723
MS.gene94094.t1 AT1G38065 71.897 427 115 1 139 560 3 429 0.0 657
MS.gene94094.t1 AT1G38065 74.442 403 103 0 158 560 37 439 0.0 654
MS.gene94094.t1 AT2G01480 52.080 553 234 7 24 557 9 549 0.0 585
MS.gene94094.t1 AT1G35510 54.344 541 208 9 46 559 28 556 0.0 575
MS.gene94094.t1 AT1G14970 52.651 547 237 5 24 559 17 552 0.0 572
MS.gene94094.t1 AT2G01480 53.010 515 216 6 24 519 9 516 0.0 557
MS.gene94094.t1 AT1G14970 58.581 437 171 2 123 559 6 432 0.0 542
MS.gene94094.t1 AT1G14970 60.688 407 155 1 153 559 5 406 0.0 530
MS.gene94094.t1 AT4G38390 42.708 384 204 6 141 523 107 475 1.08e-97 308
MS.gene94094.t1 AT4G24530 39.686 446 229 12 139 559 86 516 5.10e-96 303
MS.gene94094.t1 AT5G65470 39.019 469 231 14 124 559 56 502 1.46e-94 299
MS.gene94094.t1 AT1G29200 41.628 430 216 9 158 559 71 493 1.66e-93 296
MS.gene94094.t1 AT1G29200 41.628 430 216 9 158 559 71 493 1.66e-93 296
MS.gene94094.t1 AT1G29200 41.628 430 216 9 158 559 187 609 1.87e-92 296
MS.gene94094.t1 AT1G29200 41.549 426 214 9 162 559 1 419 1.06e-91 289
MS.gene94094.t1 AT3G26370 37.296 429 249 9 139 559 139 555 4.87e-91 291
MS.gene94094.t1 AT4G16650 44.385 374 177 9 158 523 123 473 3.60e-88 283
MS.gene94094.t1 AT4G16650 44.385 374 177 9 158 523 123 473 3.60e-88 283
MS.gene94094.t1 AT1G22460 40.000 420 215 12 159 562 162 560 5.11e-88 284
MS.gene94094.t1 AT1G11990 38.865 458 231 12 123 559 159 588 2.49e-87 283
MS.gene94094.t1 AT1G62330 36.498 474 256 11 123 562 190 652 5.80e-87 283
MS.gene94094.t1 AT1G20550 42.577 357 185 6 160 513 112 451 2.27e-86 279
MS.gene94094.t1 AT1G76270 40.691 376 203 6 141 513 90 448 2.66e-86 279
MS.gene94094.t1 AT1G11990 36.345 498 239 13 116 559 137 610 7.79e-84 274
MS.gene94094.t1 AT1G14020 37.864 412 226 10 158 559 92 483 8.32e-83 268
MS.gene94094.t1 AT5G64600 37.671 438 229 13 140 559 103 514 2.43e-82 268
MS.gene94094.t1 AT2G03280 38.235 374 209 7 158 525 89 446 6.42e-78 254
MS.gene94094.t1 AT5G15740 37.226 411 233 10 158 561 107 499 2.52e-76 251
MS.gene94094.t1 AT5G01100 35.971 417 227 11 158 559 217 608 4.87e-76 254
MS.gene94094.t1 AT3G54100 36.496 411 230 12 158 559 223 611 1.35e-74 250
MS.gene94094.t1 AT3G02250 37.772 368 208 7 158 519 107 459 1.48e-74 247
MS.gene94094.t1 AT2G37980 36.983 411 228 12 158 559 227 615 2.92e-74 249
MS.gene94094.t1 AT1G04910 36.277 419 217 11 158 559 89 474 8.16e-73 242
MS.gene94094.t1 AT5G35570 34.534 472 262 13 114 568 211 652 2.96e-72 244
MS.gene94094.t1 AT2G03280 35.661 401 209 8 158 525 89 473 3.44e-72 240
MS.gene94094.t1 AT5G63390 34.043 423 259 6 139 559 144 548 4.41e-71 239
MS.gene94094.t1 AT2G44500 33.258 445 272 10 119 559 138 561 3.60e-64 221
MS.gene94094.t1 AT3G07900 32.850 414 249 8 160 569 191 579 2.65e-62 216
MS.gene94094.t1 AT3G26370 36.929 241 140 5 139 372 139 374 8.25e-48 172
MS.gene94094.t1 AT1G52630 30.707 368 222 10 126 489 45 383 3.91e-46 169
MS.gene94094.t1 AT1G52630 30.218 321 193 8 173 489 2 295 1.35e-38 145
MS.gene94094.t1 AT3G03810 30.623 369 231 9 109 464 42 398 5.94e-37 146
MS.gene94094.t1 AT3G03810 30.623 369 231 9 109 464 42 398 5.94e-37 146
MS.gene94094.t1 AT3G30300 31.529 314 187 11 158 456 101 401 2.33e-32 132
MS.gene94094.t1 AT3G30300 31.529 314 187 11 158 456 101 401 2.33e-32 132
MS.gene94094.t1 AT2G44500 32.824 262 158 7 139 396 157 404 2.88e-28 117
MS.gene94094.t1 AT1G51630 26.287 369 217 10 137 500 75 393 2.85e-24 106
MS.gene94094.t1 AT3G21190 26.513 347 204 9 159 500 92 392 1.37e-23 103

Find 146 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGGAAACATCTTTATTAA+AGG 0.215473 5.4:-46458177 None:intergenic
GATTTGCATCATGAACATTT+TGG 0.253855 5.4:+46455220 MS.gene94094:CDS
GGCGGTGGCGGAGGCGGAGG+TGG 0.258644 5.4:+46454365 MS.gene94094:CDS
CTCTTCCTGGTTCTCGTTAC+CGG 0.260601 5.4:+46454606 MS.gene94094:CDS
ACAACAAGTTTGCGTTTATC+AGG 0.270838 5.4:-46458977 None:intergenic
ACATCAATATATTTAGCTTC+TGG 0.271700 5.4:+46458123 MS.gene94094:CDS
ATTGGTCATACTCCTCTTCC+TGG 0.278695 5.4:+46454593 MS.gene94094:CDS
TGTAATGCTGTGGCTGTTGC+TGG 0.286549 5.4:+46455862 MS.gene94094:CDS
TCGCTACTTCTTTCTTCTTC+CGG 0.297872 5.4:+46454514 MS.gene94094:CDS
ATATCATGCAGAAAAGTATT+TGG 0.315152 5.4:+46458152 MS.gene94094:CDS
TTTATCAGGAATAATGGTTT+TGG 0.315630 5.4:-46458963 None:intergenic
GAATTATCAGAATCAATTTC+AGG 0.320818 5.4:-46454656 None:intergenic
TTTGCGTTTATCAGGAATAA+TGG 0.323925 5.4:-46458969 None:intergenic
GGCGGAGGCGGAGGTGGAGG+TGG 0.328837 5.4:+46454371 MS.gene94094:CDS
AGAACTTCCTGAGGCAATAA+TGG 0.334683 5.4:+46456999 MS.gene94094:CDS
GTCATACCGCAGAGCGTTGC+TGG 0.339373 5.4:-46455746 None:intergenic
CATCAATATATTTAGCTTCT+GGG 0.347457 5.4:+46458124 MS.gene94094:CDS
TTTCCGTTCACGTTCTTCAT+TGG 0.354202 5.4:+46454575 MS.gene94094:CDS
TACATTTACGGAAACGGTTA+CGG 0.355604 5.4:+46454447 MS.gene94094:CDS
TGCTGTGCATCTTCGCTTTG+AGG 0.361326 5.4:+46457459 MS.gene94094:CDS
GGTGGCGGAGGCGGAGGTGG+AGG 0.368387 5.4:+46454368 MS.gene94094:CDS
AATGGCGGTGGCGGAGGCGG+AGG 0.369247 5.4:+46454362 MS.gene94094:CDS
AGCTCCATTTGCTAATCGTT+TGG 0.369656 5.4:+46457291 MS.gene94094:CDS
GCCTCCGCCACCGCCATTCT+CGG 0.378440 5.4:-46454357 None:intergenic
TTCCTCCCAATTGGCAGCAT+TGG 0.382578 5.4:+46458838 MS.gene94094:CDS
ATCTGCACTAGCTGAGAATC+TGG 0.385628 5.4:+46457390 MS.gene94094:CDS
CTTCCGGTGGTTTATCTCTC+CGG 0.387750 5.4:+46454530 MS.gene94094:CDS
TGGAGGAAAAGCAGAGAAGT+TGG 0.387856 5.4:+46457634 MS.gene94094:CDS
TATCAGGAATGTCTACATTA+TGG 0.388986 5.4:-46461824 None:intergenic
AGACTCCTTGGTATAAAGAT+TGG 0.391718 5.4:-46458196 None:intergenic
CACCATAGAGAAATCTTCTA+TGG 0.392874 5.4:-46458934 None:intergenic
GAGAATGGCGGTGGCGGAGG+CGG 0.393770 5.4:+46454359 MS.gene94094:CDS
GCTCCTGTCAGCTATTACCT+TGG 0.397055 5.4:+46457075 MS.gene94094:CDS
TGCTGTAGACTGTAGTTATC+AGG 0.403998 5.4:-46461840 None:intergenic
ATAAAAGGAGCAAGCTCATC+TGG 0.419795 5.4:-46458228 None:intergenic
TCCTGTAGGCATCTACATTC+TGG 0.420118 5.4:-46461777 None:intergenic
GCCGAGAATGGCGGTGGCGG+AGG 0.424599 5.4:+46454356 MS.gene94094:CDS
AAAAGCAGAGAAGTTGGAAA+TGG 0.428584 5.4:+46457640 MS.gene94094:CDS
TCCTCCATCATATACACAAC+AGG 0.434053 5.4:-46457618 None:intergenic
GACTCCTTGGTATAAAGATT+GGG 0.435088 5.4:-46458195 None:intergenic
TCAGTGTCCCCTGGCCTGAA+TGG 0.442293 5.4:+46455690 MS.gene94094:intron
GGCATCTACATTCTGGTAAA+GGG 0.445482 5.4:-46461770 None:intergenic
GGTGGAGATCGCGGTGGACT+CGG 0.448647 5.4:-46454407 None:intergenic
CACTAAGCATGTCATCCATC+TGG 0.452576 5.4:-46461677 None:intergenic
TGTTCATCCTATCTTGCAAA+AGG 0.458231 5.4:+46457101 MS.gene94094:CDS
ATGAGGGAAGTTACCACCTT+GGG 0.458726 5.4:-46458900 None:intergenic
CCATTGACTCGATAGAGGTC+AGG 0.459450 5.4:-46457722 None:intergenic
ATGGATTCAGCTCGTGAAAA+GGG 0.461884 5.4:+46457659 MS.gene94094:CDS
ATGAAGAACGTGAACGGAAA+CGG 0.461897 5.4:-46454572 None:intergenic
ATAGGGTTATTCCTCCCAAT+TGG 0.466456 5.4:+46458829 MS.gene94094:intron
CTATGGCCCATTAGAAAATG+AGG 0.466547 5.4:-46458917 None:intergenic
GTATAACTAGAATTGCATTC+AGG 0.467924 5.4:-46455888 None:intergenic
GCGGCCGAGAATGGCGGTGG+CGG 0.467955 5.4:+46454353 MS.gene94094:CDS
TGTCTCTCCATTATTGCCTC+AGG 0.469183 5.4:-46457006 None:intergenic
TGTAGTCCAATGCTGCCAAT+TGG 0.470944 5.4:-46458844 None:intergenic
AAAGGAAAGTTCAAAAGAAA+AGG 0.475308 5.4:+46457686 MS.gene94094:CDS
TCTATAATTTCAGTGTCCCC+TGG 0.476470 5.4:+46455681 MS.gene94094:intron
GGCCATAGAAGATTTCTCTA+TGG 0.478214 5.4:+46458932 MS.gene94094:CDS
CCTGACCTCTATCGAGTCAA+TGG 0.479728 5.4:+46457722 MS.gene94094:CDS
AGGCATCTACATTCTGGTAA+AGG 0.481645 5.4:-46461771 None:intergenic
CTAGCATTAGAGTCGAAGCT+TGG 0.482366 5.4:+46457052 MS.gene94094:CDS
AACGGTTACGCGGCCGAGAA+TGG 0.483832 5.4:+46454344 MS.gene94094:CDS
CTCACTAACCTCAATGAAAG+AGG 0.485605 5.4:-46454678 None:intergenic
CTTCAGTGTGGAAATACAAA+AGG 0.485641 5.4:+46455161 MS.gene94094:CDS
AATGGATTCAGCTCGTGAAA+AGG 0.486427 5.4:+46457658 MS.gene94094:CDS
TAGCATTAGAGTCGAAGCTT+GGG 0.494058 5.4:+46457053 MS.gene94094:CDS
GAGAAGAGCTCGAGGACAGA+TGG 0.496882 5.4:+46457427 MS.gene94094:CDS
TTGCAGTTGGAGTGCTCTCA+AGG 0.498262 5.4:+46461653 MS.gene94094:intron
TAGGGACCATGATTCCTTTG+CGG 0.503683 5.4:-46461707 None:intergenic
CATCCTATCTTGCAAAAGGA+AGG 0.504394 5.4:+46457105 MS.gene94094:CDS
CGGACGAAGTCACTGTCGGC+GGG 0.506458 5.4:+46454470 MS.gene94094:CDS
TACGCGGCCGAGAATGGCGG+TGG 0.508173 5.4:+46454350 MS.gene94094:CDS
CATCTTAGAAATTGTATATG+TGG 0.509769 5.4:-46457328 None:intergenic
TCCTACACACATCAACAAAC+CGG 0.512142 5.4:-46454549 None:intergenic
TGGGATGATGCTACGTGGCA+TGG 0.512796 5.4:+46458086 MS.gene94094:CDS
CAAGCGAATAAATACCTCTA+GGG 0.518400 5.4:-46457762 None:intergenic
AATACCTCTAGGGGAGTTAG+TGG 0.523241 5.4:-46457752 None:intergenic
GTAGTCCAATGCTGCCAATT+GGG 0.524395 5.4:-46458843 None:intergenic
TGATAATTCCTCTTTCATTG+AGG 0.528251 5.4:+46454670 MS.gene94094:CDS
GATCGAAAACCTCGTGACTC+CGG 0.529800 5.4:-46454625 None:intergenic
GGGATGATGCTACGTGGCAT+GGG 0.530350 5.4:+46458087 MS.gene94094:CDS
ACCAGAATGTAGATGCCTAC+AGG 0.530789 5.4:+46461776 MS.gene94094:CDS
CATAGAAGATTTCTCTATGG+TGG 0.537641 5.4:+46458935 MS.gene94094:CDS
TTGCTGGTTAAGACCACCAT+TGG 0.539892 5.4:-46455730 None:intergenic
AAGTGACCGCAAAGGAATCA+TGG 0.542741 5.4:+46461701 MS.gene94094:CDS
TGGTGTTGCAAGAGACTCCT+TGG 0.543034 5.4:-46458208 None:intergenic
ATTGCCAAACGATTAGCAAA+TGG 0.544098 5.4:-46457295 None:intergenic
TTACTAATTATCAAGCATTG+AGG 0.545752 5.4:+46457353 MS.gene94094:CDS
TGTAAAACGCCGGACTGTCG+TGG 0.562232 5.4:-46454428 None:intergenic
CGATCTCCACCACGACAGTC+CGG 0.562556 5.4:+46454419 MS.gene94094:CDS
TCGAAGACACATAACGGAAA+CGG 0.564967 5.4:+46454326 MS.gene94094:CDS
CTACTTCTTTCTTCTTCCGG+TGG 0.565538 5.4:+46454517 MS.gene94094:CDS
CGGTAACGAGAACCAGGAAG+AGG 0.571794 5.4:-46454605 None:intergenic
ATGAAATCGAAGACACATAA+CGG 0.573992 5.4:+46454320 MS.gene94094:CDS
TTCTCCTGTTGTGTATATGA+TGG 0.575030 5.4:+46457614 MS.gene94094:CDS
ACATTGTGAAATTCAAAATG+AGG 0.581885 5.4:-46455910 None:intergenic
GCTCGTGAAAAGGGGTGGAA+AGG 0.582006 5.4:+46457668 MS.gene94094:CDS
GAGTGCTCTCAAGGACCAGA+TGG 0.582704 5.4:+46461662 MS.gene94094:CDS
GACTGTCGTGGTGGAGATCG+CGG 0.582770 5.4:-46454416 None:intergenic
CTTAGAAATTGTATATGTGG+AGG 0.583613 5.4:-46457325 None:intergenic
TGACTCCGGTAACGAGAACC+AGG 0.583762 5.4:-46454611 None:intergenic
TTGATTGTCGAGGCCAATGG+TGG 0.583847 5.4:+46455717 MS.gene94094:CDS
TATTGACAAGACTCTCCTGT+AGG 0.584214 5.4:-46461791 None:intergenic
CACAGTGTGCTTGTATAGTG+AGG 0.594547 5.4:+46458865 MS.gene94094:CDS
TTAGAAATTGTATATGTGGA+GGG 0.595768 5.4:-46457324 None:intergenic
GTTTCCCAATCTTTATACCA+AGG 0.595788 5.4:+46458191 MS.gene94094:CDS
ATAGAAGATTTCTCTATGGT+GGG 0.596640 5.4:+46458936 MS.gene94094:CDS
TTCTCGTTACCGGAGTCACG+AGG 0.598162 5.4:+46454616 MS.gene94094:CDS
TCTGTAGATTTGTAATGCTG+TGG 0.604486 5.4:+46455852 MS.gene94094:intron
AATGAGGGAAGTTACCACCT+TGG 0.605838 5.4:-46458901 None:intergenic
TATGGCCCATTAGAAAATGA+GGG 0.607756 5.4:-46458916 None:intergenic
TGTGCATCTTCGCTTTGAGG+AGG 0.608488 5.4:+46457462 MS.gene94094:CDS
ATCCTATCTTGCAAAAGGAA+GGG 0.608881 5.4:+46457106 MS.gene94094:CDS
ACTCCAAGGTAATAGCTGAC+AGG 0.611615 5.4:-46457078 None:intergenic
ACAATGTGTGGAAAGATCCA+AGG 0.613128 5.4:+46455926 MS.gene94094:CDS
GGACGAAGTCACTGTCGGCG+GGG 0.622339 5.4:+46454471 MS.gene94094:CDS
TCCGGTTTGTTGATGTGTGT+AGG 0.625329 5.4:+46454548 MS.gene94094:CDS
GTCCAATGCTGCCAATTGGG+AGG 0.626792 5.4:-46458840 None:intergenic
TGACCAATGAAGAACGTGAA+CGG 0.630711 5.4:-46454578 None:intergenic
TGGATTCAGCTCGTGAAAAG+GGG 0.631678 5.4:+46457660 MS.gene94094:CDS
GGTTACGCGGCCGAGAATGG+CGG 0.633753 5.4:+46454347 MS.gene94094:CDS
AAACCGGAGAGATAAACCAC+CGG 0.637048 5.4:-46454533 None:intergenic
ACGGCGGACGAAGTCACTGT+CGG 0.644805 5.4:+46454466 MS.gene94094:CDS
AAGCGAATAAATACCTCTAG+GGG 0.646730 5.4:-46457761 None:intergenic
CTTCACATAACCATCAAGTG+TGG 0.650369 5.4:-46456968 None:intergenic
ATTCAGCTCGTGAAAAGGGG+TGG 0.650960 5.4:+46457663 MS.gene94094:CDS
AGATAGGATGAACAACTCCA+AGG 0.653627 5.4:-46457092 None:intergenic
CTTAGTGAAAGTGACCGCAA+AGG 0.653957 5.4:+46461693 MS.gene94094:CDS
ATTTACGGAAACGGTTACGG+CGG 0.654253 5.4:+46454450 MS.gene94094:CDS
GCGGACGAAGTCACTGTCGG+CGG 0.657648 5.4:+46454469 MS.gene94094:CDS
TCCTGTTGTGTATATGATGG+AGG 0.660944 5.4:+46457617 MS.gene94094:CDS
AAATCAGTCAAATTCGAACA+AGG 0.661890 5.4:-46455202 None:intergenic
ATACCTCTAGGGGAGTTAGT+GGG 0.676100 5.4:-46457751 None:intergenic
TCTTAACCAGCAACGCTCTG+CGG 0.677605 5.4:+46455740 MS.gene94094:CDS
AAAACGCCGGACTGTCGTGG+TGG 0.678207 5.4:-46454425 None:intergenic
GAATGAGTGAGAAGAGCTCG+AGG 0.678527 5.4:+46457419 MS.gene94094:CDS
ATGCCCACTAACTCCCCTAG+AGG 0.680140 5.4:+46457748 MS.gene94094:CDS
ACATAACGGAAACGGTTACG+CGG 0.684145 5.4:+46454334 MS.gene94094:CDS
ACTTGATGGTTATGTGAAGG+TGG 0.685624 5.4:+46456972 MS.gene94094:CDS
GGTGGTTAAAGAACTTCCTG+AGG 0.691083 5.4:+46456990 MS.gene94094:CDS
TTTCCGTAAATGTAAAACGC+CGG 0.692591 5.4:-46454438 None:intergenic
CACACTTGATGGTTATGTGA+AGG 0.705447 5.4:+46456969 MS.gene94094:CDS
TGTCGGCGGGGATAATGACA+CGG 0.710205 5.4:+46454483 MS.gene94094:CDS
TGTCGTGGTGGAGATCGCGG+TGG 0.714280 5.4:-46454413 None:intergenic
CGGCGGGGATAATGACACGG+TGG 0.779770 5.4:+46454486 MS.gene94094:CDS
TAGGTTGGGATGATGCTACG+TGG 0.784598 5.4:+46458081 MS.gene94094:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.4 gene 46454320 46461884 46454320 ID=MS.gene94094
chr5.4 mRNA 46454320 46461884 46454320 ID=MS.gene94094.t1;Parent=MS.gene94094
chr5.4 exon 46454320 46454691 46454320 ID=MS.gene94094.t1.exon1;Parent=MS.gene94094.t1
chr5.4 CDS 46454320 46454691 46454320 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
chr5.4 exon 46455157 46455241 46455157 ID=MS.gene94094.t1.exon2;Parent=MS.gene94094.t1
chr5.4 CDS 46455157 46455241 46455157 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
chr5.4 exon 46455694 46455761 46455694 ID=MS.gene94094.t1.exon3;Parent=MS.gene94094.t1
chr5.4 CDS 46455694 46455761 46455694 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
chr5.4 exon 46455859 46455947 46455859 ID=MS.gene94094.t1.exon4;Parent=MS.gene94094.t1
chr5.4 CDS 46455859 46455947 46455859 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
chr5.4 exon 46456930 46457127 46456930 ID=MS.gene94094.t1.exon5;Parent=MS.gene94094.t1
chr5.4 CDS 46456930 46457127 46456930 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
chr5.4 exon 46457279 46457483 46457279 ID=MS.gene94094.t1.exon6;Parent=MS.gene94094.t1
chr5.4 CDS 46457279 46457483 46457279 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
chr5.4 exon 46457602 46457769 46457602 ID=MS.gene94094.t1.exon7;Parent=MS.gene94094.t1
chr5.4 CDS 46457602 46457769 46457602 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
chr5.4 exon 46458084 46458251 46458084 ID=MS.gene94094.t1.exon8;Parent=MS.gene94094.t1
chr5.4 CDS 46458084 46458251 46458084 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
chr5.4 exon 46458833 46459023 46458833 ID=MS.gene94094.t1.exon9;Parent=MS.gene94094.t1
chr5.4 CDS 46458833 46459023 46458833 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
chr5.4 exon 46461659 46461884 46461659 ID=MS.gene94094.t1.exon10;Parent=MS.gene94094.t1
chr5.4 CDS 46461659 46461884 46461659 ID=cds.MS.gene94094.t1;Parent=MS.gene94094.t1
Gene Sequence

>MS.gene94094

ATGAAATCGAAGACACATAACGGAAACGGTTACGCGGCCGAGAATGGCGGTGGCGGAGGCGGAGGTGGAGGTGGAAGTAGTAGCTCACCGAGTCCACCGCGATCTCCACCACGACAGTCCGGCGTTTTACATTTACGGAAACGGTTACGGCGGACGAAGTCACTGTCGGCGGGGATAATGACACGGTGGAGTCTTCGCTACTTCTTTCTTCTTCCGGTGGTTTATCTCTCCGGTTTGTTGATGTGTGTAGGACCGTTTCCGTTCACGTTCTTCATTGGTCATACTCCTCTTCCTGGTTCTCGTTACCGGAGTCACGAGGTTTTCGATCATCTTTGGCCTGAAATTGATTCTGATAATTCCTCTTTCATTGAGGTTAGTGAGATTAAGCGTAATGTACAATAATCGCGTTTGGTGAATAATTTTGTTTATGGATTTGGTGCTATATGAAGTTTTATGATGGAGCTGAATTCGTGTTACTAAATCTTGTTTGGTGAATAATTTTGGATTATTATGGAGCTGAATTCCTGTTACTGAATCTTGTTTGGTGAATAATTTTGGCGTTACTAAATCTTGTTTGGTGAATAATTTTGGATTATTATGTTGCTGAATTCGTGTTACTGAATCTTGTTTGTTGAATAATTTTGGCGTTACTAAATCTTGTTTGGTGAATAATTTTGTTTTTGGTGTTATTTGAAAATTGGTTGTTTTGTTGATTTTGTTGTTTATTATTAACTACTTATTATAGATGCGTGATTAATAATGAAATTATTTTTGTCGATAAATAAACAGGGTTGATTTTTAATTGTTTATTTATTTATTTATTTATTTATTTTGCAGTTGTCTTCAGTGTGGAAATACAAAAGGAAATTGAGAGAGAAGAAGCCTTGTTCGAATTTGACTGATTTGCATCATGAACATTTTGGTATATAATACTAAAGCTCTATGATCTATTCTCTCTTAGCTTTCGTTTCACATGTCTACCTGTTTGACTCAGACATTGGATATGATATTGACACTGACACTGATAATAATTTGAGAAAACGATATTATTCAATGTAATCATATGTGTTGGTATTGTGTTGTGTCAGACTTGCTTTCGATCAAAGATGTCGGTGCTACATAGTTTAATATTGCTGCTAATGTGTGATTTTGCTTTGTAATATAGTCAACTTTATTTAAAAGTTCTTATCAAAATAGCTGATGAATCATAAGTTGTTTTAAGCTGATTTTCATAAGCTCTGTTGTAGAAATAGTTTATATAGAATTCATGCAGAATTTGCTCCATTTCCACTTTGATCGTAACCTTATATGATAAGCTGCTAGTTAGTTATTTATGGAAAATGCTCTCTAATATTATGTTTTCTATAATTTCAGTGTCCCCTGGCCTGAATGGTTTTTTGATTGTCGAGGCCAATGGTGGTCTTAACCAGCAACGCTCTGCGGTATGACATAGTTTTTCCTTTGTCTGCCTTTTGGCAATTTTTTTATTCGACGGATGAACTATTTTTAACCGTTGTGATTTTAATTGTGGCTCTGTAGATTTGTAATGCTGTGGCTGTTGCTGGACTCCTGAATGCAATTCTAGTTATACCTCATTTTGAATTTCACAATGTGTGGAAAGATCCAAGGTGACCAATTTACCTATTTATTATCTTTGGCTTGAGAATTATTTTCAACACAAACATTGCTAGTTAAGTTTCATTGTTTGGCCTGTTTTTGTCAAAATATTACCACATGTGGTTGGGGAGAGAATGAGTTTATCCGTTGCTTAATACAGTAGGTGTGTGTTAGCTTTATACTCTTGTTTATGCAAACTAATAATGTGCTTTGATGCAATTGTTTATGAACTGAAATTGTCTTCCATCAAATGGCTATTTACAATATTTTTAAATCTAAAACAAGGTACTCACTGCTGCCTTTGTTAGGCCGATTTTTCTATCTATCAGAATTGTATAATGACAACTCTTCAGGAAAAAATTGATAGCGAATGGAGAAAGATTTTCAATTTAAGAGAGGAAATGTTTTTGCTAGGTTGTTATCATTCAATTGGAGGGATAAGGAATGAAATGGGAATAATGGATTTGAATCCTTTCCTAAGAGAACTCATATCACATGCCAAAAAGAAAAGAAGAAAATAGAATTTCATTCAATGGTTTTTAAGAAACTTTCCTACCATGTAACTCATAAAATATTTGATGTAAATATGTTTCAATAGGTTGTTTATTTAATCATTTTTCCCATGAACAGTTCCATATCACATTGCTTTTTATGTTATAAGTAGTGCATTAATTTTACTTCCTATACAAAACCTACGCTAGAGGCTGATTCTCCTACGATATACTTTTTTGAATTACATTAGTTAGCACTTCTAACTTTATGGTATGTTTATGATGCGGTTGATCAAGCATTACTTGTTTAACGTTTGTCTTCAACTAGTAGATACTGAAATCATACCTCATTTGATGTGTGTATTTTCTCGTAGAACCATCCATACATATAATTAATTATTAAATATACCCGTAGTCAACAATACGAGGCTAATTAAACTTTTATGCCTGATTTCATTTCTGCATTCACTATCTATTTTAAATAATGTTATTTTGTGTACAGTGAATTTGGGGATATATATGATGAAGATCATTTTATATCCACACTTGATGGTTATGTGAAGGTGGTTAAAGAACTTCCTGAGGCAATAATGGAGAGACATAATTACAATATGACTAATATAACTAGCATTAGAGTCGAAGCTTGGGCTCCTGTCAGCTATTACCTTGGAGTTGTTCATCCTATCTTGCAAAAGGAAGGGTAGGTATTAGACCACTTTGCCCTCATTTCTTATTCTCTGTCTTGGTTTTGGAGTGAGCTTGTACTGTGAATCCATATTAGGCAGTCGACACAACTCAATTTTAAGCAAACAATTTGATGAAAGGAACTTTATTTTGGGAAACTTATGCAGGGTTATTCGAATAGCTCCATTTGCTAATCGTTTGGCAATGAGTGTCCCTCCACATATACAATTTCTAAGATGTCTTACTAATTATCAAGCATTGAGGTTTTCTTCTTCAATATCTGCACTAGCTGAGAATCTGGTTTATCGAATGAGTGAGAAGAGCTCGAGGACAGATGGAAAATATATTGCTGTGCATCTTCGCTTTGAGGAGGTACTGATGCTCATTCTATCATATTGAAAATTAGGTTTTGCTATATGCATCTCTAGGTGTCAAAAGTATACAGAATTGAAGTATTATGTAATAATTGCCTAACTATCCTAACATACAGGACATGATAGCATTCTCCTGTTGTGTATATGATGGAGGAAAAGCAGAGAAGTTGGAAATGGATTCAGCTCGTGAAAAGGGGTGGAAAGGAAAGTTCAAAAGAAAAGGTCGTATCGTTGTACCTGACCTCTATCGAGTCAATGGAAAATGCCCACTAACTCCCCTAGAGGTATTTATTCGCTTGCTGATTCTTCTCATATCTTGAATTATTTTTTCCCTTAATTTAAAATCTCGCCAGTCTCATGTCCTGAAGTTATTGATTTCTTAATGAGATATTGCTTTTCTTTTTTCCCTTAATTTATTCTTGATTCCAATTGATTATGGCCTCATCTTTGTGCACTGTTTTAAATTAATTTTTGTAGAAGAGCAACATATTGAGATGAAAATCAAACAAGTCTATGGAAGTTTAAATTGTGATAAAACTGAGATTATATTATGGAAGAGACTGATGTTTATTTCCTGTTGATTTCCATAATTATGTAGGTTGGGATGATGCTACGTGGCATGGGATTCAGTAACAATACATCAATATATTTAGCTTCTGGGAAAATATATCATGCAGAAAAGTATTTGGCTCCTTTAATAAAGATGTTTCCCAATCTTTATACCAAGGAGTCTCTTGCAACACCAGATGAGCTTGCTCCTTTTATGGTAAAAATCTTTACTGGTGAAGAAAATATATATTTCATTCTTCCATTCCATGACCTTGCTGTGATAAAATTTATAACTAATGAATTGGACTTAGTGGCATGGAGTTGTAACTTATAACAACCCTTCATTAAAACTCTTAAATTTGGGTTGGAGTTGGACCTAGGAACCGAAGTTCATCCATTTTAAAAGAGGATCACCAGTTTGAACTTCGATTGGTTCATTTTTTTTTGGAATTATAGCTGATGCTCCAACACCAAGATTGCATTACTGGAAGGAAACCTGAATATTGTAATATTTATAATAATCAAGGATGTAGTTAACTTTCAATTCCAAAATATAAATGATGTATTTGGCTTTTTATTGTGGTTCTCTCTTCATCCCTCATTCTAATACTTAGTATATTTGGCATGATGTTTAGTTGTTAACATCTTTACTGCTCTCTGTGTGACTGTGAGGTATAATGTAAAAAAAAAATAAAATAAAATCACGGGCAGGTTTAGTTATTCTTTCTTTAAGTGTATGCCTTAATATTTTGTGTGATTTTCGAATATGCTCTTATGCTTAGTTTCATCCTTCTATAGGGTTATTCCTCCCAATTGGCAGCATTGGACTACACAGTGTGCTTGTATAGTGAGGTTTTTGTTACAACCCAAGGTGGTAACTTCCCTCATTTTCTAATGGGCCATAGAAGATTTCTCTATGGTGGGCATGCCAAAACCATTATTCCTGATAAACGCAAACTTGTTGTTCTTTTTGAGAACACGACTATCAGGTACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCTCCCTCTCTCTCACTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAAGATTTAGTGGAATACCATATATCATTTTCGTTGTCTTTTTAGAAATATAATAAAACTTTAGGTGATATGTATCTAGGATTCATATTATAATTTCCAGCCTGTTTTATGACATGAGTGCTAACTGATTGAAATATCCCCTAGACATGAATGAGAACCTTTTGCTATGCACTAGGTAAAATTGAGAAACAGATAATTTTTTTTCTATTATACCTTCTTTTAACTAAAATAGGATGCAGCCAAACTCACGAGATCATTTACAAGTACTAGTTATACTCTATTTATTATCTACTTATCTTGAAAAATCAAAATTGTTTTGAGAAAGTTGTGACCAATTAAGTAAAAGTGAAGGAGCAAAGATCTAAATCATTATTAATGGTAATCCTCTTGACGGTCCTACTTTTGGTTCCCTCTCTACTTTAATTTGCAAGACTAATCGATCTAAGAAGACCGAATTTAAGCATAGCTTTTGTATGTCTGTCTCAAACAAGTTTTCGTATAGACTTGAAGCATGGCTTCCAGAAAAGGTTACTAAAATGAAAGGATCATGTACCCTGAGTTGCTAACTTGGCGGTGCTCACCATGATTAACGATTAGAAACCAAAAGCTAGACCAGACATACCTTTGTAGACGTAGCCCCAGTTGGTTTCTTGTAGTTTTGCAATTGTTTGTAACATGGTTTTTGCCAAACAATAACTAAAATAATATTAAGCTCAACAAATAACACAAATTTAAACAACCAAGAGAACACATATCAAATATTAGGATTCAATCATGATTCATAAATTAGGATTCATTGTTGTCCTATCCAAAGGGCTTAACAATGCTTAATAATATCCTTAAAAACAATAGCAAGAATGTCATATCCTCAAATATCAAAATAAAATATAAAAGAAAAGGGTTCACGAACAAAGCCTATTGGAACCCGAGAAATAGTTCCCTTACAATCACCTTGAAAGACTTAGAAATCAATCAAGAAATATCAAAGGTTGCTCGTCGAAATCATTATGAAATTGCAATTGAAGTTGTCACTTGTCACCATCACGTCATCTATCAGTTGTTACTTCTCTGCGTTGTTATCGTGTTATCAGCATGTTAGCTTGTTTGTGTCATTAGGTTTTTTGGGTTTTCAAGAACTAGAGTATTATAAGGAGAAATAGCCTTTGAAATTGAGCTTCCATATAGTTGAATGTCCGTTGAACATAAAGGGAATGCCTTGGCACATACCACCTTACGCATAAATCTGCATTTCTATGCTGAATGTTTCACTTCCGTGCAGGTCTCCAAAGCAACTCCTTTGCATTCCAAAGTGTAGATTTCTTCATATCTTCATTTTAGCCCAAAATCTCCAATTTTCGTCTTGTTTTAACCATGTTTAAGTAATTCAATTCCACTCCTCCTAATGCCAATCTTCAGTGGTAGCTGAATTTCAAATCCAACTAAGAACACCTTGGAATCCTTAACTCGTCCAGTAATTCCTCTATAACTAGAAAGGGGGATTTGTTGGGTATTGTAACTTTGTTTAGACTAGAGGCTCTGTAATTCATAAAAGATTCTCAGCCGCCACTTTTTTCTGCACTAATATCATGGGACTCGAATAACAACTTACACTAGGCCGAGTGATGCCTGCAGCTAGATGTAAGCAATTCGAACTGAGCATCTAAGATTTGAATTTGGCTTGTGAAAAAAAGAGTTGCTCAAGTTTGACTTATTTATTTAAACGAGTCTAACTAGAAACATGAGTTTGACTTGTTAAAAAAGTGGCCTTGACTTTTTTTTTTTTTTAAATAAATAAGCAAAGTGGGCTTGACTTGTTTTGCTATTTTATAACTTACTGTTTTGCATTTAGAATAAATTTTCTTAAATTACTTTTGAAGCTAATCAATTTAGTATTTAATAAATATTTAATATTATAAGCAAATCTACAAATAGAAATATGTGATTAAGTAAACCCAATCTTAATTTGTATGCCTCATTCTAATATACAAGACTTTAATATTTATACATGTATATTTGGAGGGATCAAATTACACCAGTGTCATACTTGGATATTGTTGCACTGTTTAATAGTTTTTTTTTTCTTTTTCCTTTCAGATACTTGTATTACAAAACTCACCGTTGGATAAGTTATTATTGTTTATATCATTTATGTAAATTCTTACGTAAATATAAAATCATTTTATATGTCAATGAGATATTTACGGGTTAACATTGTTTGATAAAAGTCCATAAGTCGTTAATCTTGATGTATCCTCAGCATCTCTCCTATCAGAACCGGACAGAACAAGCTGTTCTGATCTGTGTCCAATGTCCAAGTCCCAAGTGCTTTCTCTAATTGTTTGTTTATGTCTGTGAATTAAAATTGCCAATGAGTTTTATACTGGATTTGTTTCCCATCCTTGTCTGCATCTTATATTTAAGCATAGTTGCCATGTGGTTGGATTCAATAGGAAAGCTTAGGCTATGCAATCTTTGAAACACACAAGATGACACAATATTTGTGTATAAATTTTGCAGTTGGAGTGCTCTCAAGGACCAGATGGATGACATGCTTAGTGAAAGTGACCGCAAAGGAATCATGGTCCCTAGAGTTAAAAAAATTAACAGAAAAACTTCTGTCTACACTTACCCTTTACCAGAATGTAGATGCCTACAGGAGAGTCTTGTCAATAAAACAATTGACCATAATGTAGACATTCCTGATAACTACAGTCTACAGCAGAACAAAAGCTTAAACAGATGA
Protein sequence

>MS.gene94094.t1

MKSKTHNGNGYAAENGGGGGGGGGGSSSSPSPPRSPPRQSGVLHLRKRLRRTKSLSAGIMTRWSLRYFFLLPVVYLSGLLMCVGPFPFTFFIGHTPLPGSRYRSHEVFDHLWPEIDSDNSSFIELSSVWKYKRKLREKKPCSNLTDLHHEHFVSPGLNGFLIVEANGGLNQQRSAICNAVAVAGLLNAILVIPHFEFHNVWKDPSEFGDIYDEDHFISTLDGYVKVVKELPEAIMERHNYNMTNITSIRVEAWAPVSYYLGVVHPILQKEGVIRIAPFANRLAMSVPPHIQFLRCLTNYQALRFSSSISALAENLVYRMSEKSSRTDGKYIAVHLRFEEDMIAFSCCVYDGGKAEKLEMDSAREKGWKGKFKRKGRIVVPDLYRVNGKCPLTPLEVGMMLRGMGFSNNTSIYLASGKIYHAEKYLAPLIKMFPNLYTKESLATPDELAPFMGYSSQLAALDYTVCLYSEVFVTTQGGNFPHFLMGHRRFLYGGHAKTIIPDKRKLVVLFENTTISWSALKDQMDDMLSESDRKGIMVPRVKKINRKTSVYTYPLPECRCLQESLVNKTIDHNVDIPDNYSLQQNKSLNR