Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94191.t1 | XP_003616734.2 | 98.7 | 76 | 1 | 0 | 1 | 76 | 1 | 76 | 2.30E-33 | 151 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94191.t1 | F4JZL7 | 44.3 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 4.1e-07 | 55.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94191.t1 | G7K7B2 | 98.7 | 76 | 1 | 0 | 1 | 76 | 1 | 76 | 1.6e-33 | 151.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053169 | MS.gene94191 | 0.811581 | 6.52E-51 | -1.69E-46 |
| MS.gene054556 | MS.gene94191 | 0.808113 | 3.63E-50 | -1.69E-46 |
| MS.gene055741 | MS.gene94191 | 0.804892 | 1.74E-49 | -1.69E-46 |
| MS.gene055744 | MS.gene94191 | 0.805699 | 1.18E-49 | -1.69E-46 |
| MS.gene055909 | MS.gene94191 | 0.822191 | 2.70E-53 | -1.69E-46 |
| MS.gene055911 | MS.gene94191 | 0.823307 | 1.49E-53 | -1.69E-46 |
| MS.gene057610 | MS.gene94191 | 0.80071 | 1.27E-48 | -1.69E-46 |
| MS.gene059807 | MS.gene94191 | 0.802823 | 4.67E-49 | -1.69E-46 |
| MS.gene060372 | MS.gene94191 | 0.817926 | 2.56E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94191.t1 | MTR_5g083710 | 98.684 | 76 | 1 | 0 | 1 | 76 | 1 | 76 | 3.71e-47 | 144 |
| MS.gene94191.t1 | MTR_3g072000 | 82.609 | 69 | 12 | 0 | 1 | 69 | 1 | 69 | 2.74e-26 | 91.7 |
| MS.gene94191.t1 | MTR_1g092670 | 32.836 | 67 | 45 | 0 | 2 | 68 | 3 | 69 | 2.69e-11 | 53.9 |
| MS.gene94191.t1 | MTR_5g055020 | 35.714 | 70 | 45 | 0 | 5 | 74 | 27 | 96 | 6.43e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94191.t1 | AT5G05365 | 75.000 | 68 | 17 | 0 | 1 | 68 | 1 | 68 | 2.05e-30 | 102 |
| MS.gene94191.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 3.20e-11 | 57.4 |
| MS.gene94191.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 3.20e-11 | 57.4 |
| MS.gene94191.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 3.20e-11 | 57.4 |
| MS.gene94191.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 4.40e-11 | 57.0 |
| MS.gene94191.t1 | AT5G19090 | 44.262 | 61 | 34 | 0 | 7 | 67 | 14 | 74 | 4.40e-11 | 57.0 |
Find 11 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTCCAATCACTTCTTCAT+TGG | 0.377427 | 5.4:+66268810 | None:intergenic |
| TTGAACAAGGTCATCGTTAC+GGG | 0.390894 | 5.4:-66268841 | MS.gene94191:CDS |
| ATTGAACAAGGTCATCGTTA+CGG | 0.498697 | 5.4:-66268842 | MS.gene94191:CDS |
| AAGGCCATCAAGAAAATTGA+AGG | 0.519646 | 5.4:-66269136 | MS.gene94191:intron |
| GTCACCAATGAAGAAGTGAT+TGG | 0.524078 | 5.4:-66268814 | MS.gene94191:CDS |
| CAGGTGGTGGAAGTGAAGGT+TGG | 0.559119 | 5.4:-66269193 | MS.gene94191:CDS |
| TCATCAGGTGGTGGAAGTGA+AGG | 0.598746 | 5.4:-66269197 | MS.gene94191:intron |
| GTGTATCAAGAAAATTCTCA+AGG | 0.630976 | 5.4:-66269155 | MS.gene94191:CDS |
| TGTCGACAAGCAATTGAACA+AGG | 0.640540 | 5.4:-66268854 | MS.gene94191:CDS |
| TGGCAAGACTGCAACTGTGT+GGG | 0.657615 | 5.4:-66268776 | MS.gene94191:CDS |
| TTGGCAAGACTGCAACTGTG+TGG | 0.701929 | 5.4:-66268777 | MS.gene94191:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAATACAAAAAAATAG+AGG | - | chr5.4:66269027-66269046 | MS.gene94191:intron | 10.0% |
| !!! | ATTATAGTAGAGTTTTGTAT+TGG | + | chr5.4:66268949-66268968 | None:intergenic | 20.0% |
| !!! | GATCATTTTATTTTTAGCTA+TGG | - | chr5.4:66268792-66268811 | MS.gene94191:CDS | 20.0% |
| !!! | TATAATTTGTCTATAATGTG+TGG | - | chr5.4:66268963-66268982 | MS.gene94191:intron | 20.0% |
| !! | ATTGGAGTTTTACATAAGAT+TGG | - | chr5.4:66269243-66269262 | MS.gene94191:intron | 25.0% |
| !! | TTTTATTGTTATGTGTCATC+AGG | - | chr5.4:66268827-66268846 | MS.gene94191:CDS | 25.0% |
| !!! | AATAATTGTTGACTCATTTG+TGG | - | chr5.4:66269138-66269157 | MS.gene94191:CDS | 25.0% |
| !!! | TTTTTGATTGACTCTTACAT+TGG | + | chr5.4:66268754-66268773 | None:intergenic | 25.0% |
| GTGTATCAAGAAAATTCTCA+AGG | - | chr5.4:66268884-66268903 | MS.gene94191:intron | 30.0% | |
| ! | GTTACCTTCAATTTTCTTGA+TGG | + | chr5.4:66268910-66268929 | None:intergenic | 30.0% |
| AACTCCAATCACTTCTTCAT+TGG | + | chr5.4:66269232-66269251 | None:intergenic | 35.0% | |
| AAGGCCATCAAGAAAATTGA+AGG | - | chr5.4:66268903-66268922 | MS.gene94191:intron | 35.0% | |
| ATTGAACAAGGTCATCGTTA+CGG | - | chr5.4:66269197-66269216 | MS.gene94191:intron | 35.0% | |
| TATTGTTATGTGTCATCAGG+TGG | - | chr5.4:66268830-66268849 | MS.gene94191:CDS | 35.0% | |
| GTCACCAATGAAGAAGTGAT+TGG | - | chr5.4:66269225-66269244 | MS.gene94191:intron | 40.0% | |
| TGTCGACAAGCAATTGAACA+AGG | - | chr5.4:66269185-66269204 | MS.gene94191:CDS | 40.0% | |
| TTGAACAAGGTCATCGTTAC+GGG | - | chr5.4:66269198-66269217 | MS.gene94191:intron | 40.0% | |
| TGTTATGTGTCATCAGGTGG+TGG | - | chr5.4:66268833-66268852 | MS.gene94191:CDS | 45.0% | |
| TGGCAAGACTGCAACTGTGT+GGG | - | chr5.4:66269263-66269282 | MS.gene94191:intron | 50.0% | |
| TTGGCAAGACTGCAACTGTG+TGG | - | chr5.4:66269262-66269281 | MS.gene94191:intron | 50.0% | |
| !! | TCATCAGGTGGTGGAAGTGA+AGG | - | chr5.4:66268842-66268861 | MS.gene94191:CDS | 50.0% |
| !! | CAGGTGGTGGAAGTGAAGGT+TGG | - | chr5.4:66268846-66268865 | MS.gene94191:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 66268747 | 66269314 | 66268747 | ID=MS.gene94191 |
| chr5.4 | mRNA | 66268747 | 66269314 | 66268747 | ID=MS.gene94191.t1;Parent=MS.gene94191 |
| chr5.4 | exon | 66269306 | 66269314 | 66269306 | ID=MS.gene94191.t1.exon1;Parent=MS.gene94191.t1 |
| chr5.4 | CDS | 66269306 | 66269314 | 66269306 | ID=cds.MS.gene94191.t1;Parent=MS.gene94191.t1 |
| chr5.4 | exon | 66269137 | 66269212 | 66269137 | ID=MS.gene94191.t1.exon2;Parent=MS.gene94191.t1 |
| chr5.4 | CDS | 66269137 | 66269212 | 66269137 | ID=cds.MS.gene94191.t1;Parent=MS.gene94191.t1 |
| chr5.4 | exon | 66268747 | 66268892 | 66268747 | ID=MS.gene94191.t1.exon3;Parent=MS.gene94191.t1 |
| chr5.4 | CDS | 66268747 | 66268892 | 66268747 | ID=cds.MS.gene94191.t1;Parent=MS.gene94191.t1 |
| Gene Sequence |
| Protein sequence |
