AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94227


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94227.t1 MTR_5g083010 98.324 179 1 1 1 179 1 177 7.01e-129 359
MS.gene94227.t1 MTR_3g071420 75.275 182 42 1 1 179 4 185 3.53e-89 259
MS.gene94227.t1 MTR_7g033800 50.667 150 70 1 8 153 8 157 1.40e-48 155
MS.gene94227.t1 MTR_4g105170 46.154 169 79 2 9 177 9 165 3.68e-47 152
MS.gene94227.t1 MTR_6g005070 64.151 106 38 0 8 113 6 111 1.30e-44 144
MS.gene94227.t1 MTR_3g077240 63.551 107 39 0 9 115 8 114 2.01e-44 145
MS.gene94227.t1 MTR_3g031660 55.000 120 54 0 8 127 4 123 1.39e-42 140
MS.gene94227.t1 MTR_5g083230 63.366 101 37 0 8 108 3 103 3.10e-42 139
MS.gene94227.t1 MTR_6g005080 61.321 106 41 0 8 113 7 112 5.83e-42 137
MS.gene94227.t1 MTR_4g083680 60.396 101 40 0 8 108 12 112 9.92e-42 138
MS.gene94227.t1 MTR_3g071590 62.376 101 38 0 8 108 5 105 1.99e-41 137
MS.gene94227.t1 MTR_2g068760 58.491 106 44 0 3 108 5 110 6.18e-41 135
MS.gene94227.t1 MTR_3g073690 60.952 105 41 0 8 112 6 110 4.57e-40 135
MS.gene94227.t1 MTR_5g017950 60.952 105 41 0 8 112 5 109 8.21e-40 137
MS.gene94227.t1 MTR_5g083960 48.529 136 69 1 8 142 36 171 5.26e-39 132
MS.gene94227.t1 MTR_8g040900 59.829 117 43 1 8 120 4 120 3.46e-38 130
MS.gene94227.t1 MTR_3g452660 48.872 133 62 2 8 140 4 130 4.37e-38 132
MS.gene94227.t1 MTR_3g094690 56.863 102 44 0 12 113 48 149 5.45e-36 125
MS.gene94227.t1 MTR_2g093310 55.882 102 45 0 10 111 32 133 8.16e-36 124
MS.gene94227.t1 MTR_7g096530 51.327 113 54 1 2 113 5 117 1.03e-35 124
MS.gene94227.t1 MTR_5g075020 61.290 93 30 1 4 90 30 122 3.85e-35 123
MS.gene94227.t1 MTR_8g036085 54.902 102 45 1 12 112 24 125 3.92e-35 123
MS.gene94227.t1 MTR_8g036105 50.442 113 55 1 1 112 8 120 5.53e-35 122
MS.gene94227.t1 MTR_5g075020 61.290 93 30 1 4 90 34 126 6.90e-35 122
MS.gene94227.t1 MTR_1g095850 55.446 101 45 0 12 112 73 173 9.26e-35 122
MS.gene94227.t1 MTR_7g074990 51.485 101 48 1 12 111 21 121 2.13e-34 121
MS.gene94227.t1 MTR_3g094690 55.882 102 44 1 12 113 48 148 1.73e-33 119
MS.gene94227.t1 MTR_1g070220 52.778 108 49 2 3 108 6 113 3.37e-33 117
MS.gene94227.t1 MTR_1g070205 46.721 122 63 2 12 131 13 134 3.56e-33 118
MS.gene94227.t1 MTR_4g060950 56.701 97 41 1 10 105 23 119 3.69e-33 117
MS.gene94227.t1 MTR_7g096610 50.893 112 53 2 8 117 5 116 4.09e-33 117
MS.gene94227.t1 MTR_6g027710 50.495 101 49 1 12 111 41 141 5.02e-33 117
MS.gene94227.t1 MTR_7g075230 50.000 108 51 2 8 112 9 116 1.42e-30 110
MS.gene94227.t1 MTR_5g080470 44.340 106 58 1 9 113 5 110 3.54e-30 109
MS.gene94227.t1 MTR_7g028905 43.689 103 58 0 10 112 4 106 1.44e-29 105
MS.gene94227.t1 MTR_8g079660 49.020 102 52 0 12 113 9 110 8.36e-29 107
MS.gene94227.t1 MTR_5g093010 42.574 101 58 0 12 112 6 106 5.44e-28 102
MS.gene94227.t1 MTR_8g079620 47.059 102 54 0 12 113 9 110 3.34e-27 102
MS.gene94227.t1 MTR_6g027700 44.898 98 53 1 12 108 8 105 4.18e-26 99.4
MS.gene94227.t1 MTR_4g099230 36.090 133 74 4 12 137 3 131 1.80e-24 93.6
MS.gene94227.t1 MTR_4g107450 31.481 162 104 2 8 169 7 161 7.26e-23 92.8
MS.gene94227.t1 MTR_8g079580 43.689 103 57 1 12 113 9 111 1.01e-22 90.9
MS.gene94227.t1 MTR_2g100020 35.455 110 71 0 8 117 16 125 2.46e-22 90.9
MS.gene94227.t1 MTR_6g011230 43.529 85 48 0 8 92 8 92 6.61e-22 88.2
MS.gene94227.t1 MTR_6g011200 37.615 109 67 1 7 114 5 113 8.30e-22 89.0
MS.gene94227.t1 MTR_8g017090 35.238 105 68 0 8 112 9 113 9.58e-21 87.0
MS.gene94227.t1 MTR_6g011250 36.697 109 68 1 7 114 5 113 4.51e-20 83.2
MS.gene94227.t1 MTR_5g085390 32.394 142 88 3 7 140 5 146 7.87e-19 80.9
MS.gene94227.t1 MTR_4g088035 31.111 90 62 0 8 97 3 92 2.02e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94227.t1 AT3G11090 80.180 111 22 0 1 111 1 111 1.40e-63 193
MS.gene94227.t1 AT5G63090 57.983 119 47 1 9 124 9 127 2.45e-44 145
MS.gene94227.t1 AT5G63090 57.983 119 47 1 9 124 9 127 2.45e-44 145
MS.gene94227.t1 AT5G63090 57.983 119 47 1 9 124 9 127 2.45e-44 145
MS.gene94227.t1 AT5G63090 57.983 119 47 1 9 124 9 127 2.45e-44 145
MS.gene94227.t1 AT3G27650 56.780 118 51 0 8 125 36 153 6.74e-44 143
MS.gene94227.t1 AT5G66870 54.015 137 54 2 8 143 4 132 8.46e-44 147
MS.gene94227.t1 AT2G30130 56.780 118 50 1 8 125 5 121 2.03e-41 138
MS.gene94227.t1 AT1G65620 54.400 125 57 0 8 132 6 130 1.43e-39 133
MS.gene94227.t1 AT1G65620 54.400 125 57 0 8 132 6 130 1.43e-39 133
MS.gene94227.t1 AT1G65620 54.400 125 57 0 8 132 6 130 1.43e-39 133
MS.gene94227.t1 AT1G65620 54.400 125 57 0 8 132 6 130 1.43e-39 133
MS.gene94227.t1 AT1G65620 54.400 125 57 0 8 132 6 130 1.43e-39 133
MS.gene94227.t1 AT2G23660 56.731 104 45 0 8 111 2 105 2.16e-39 136
MS.gene94227.t1 AT2G23660 56.731 104 45 0 8 111 2 105 2.16e-39 136
MS.gene94227.t1 AT2G23660 56.731 104 45 0 8 111 2 105 2.16e-39 136
MS.gene94227.t1 AT2G30340 52.941 119 54 1 12 130 53 169 1.22e-37 130
MS.gene94227.t1 AT2G30340 52.941 119 54 1 12 130 54 170 1.29e-37 130
MS.gene94227.t1 AT1G31320 60.000 90 36 0 8 97 10 99 1.05e-36 125
MS.gene94227.t1 AT1G07900 58.586 99 41 0 10 108 32 130 3.40e-36 124
MS.gene94227.t1 AT1G16530 42.105 152 82 3 2 151 4 151 6.19e-36 123
MS.gene94227.t1 AT2G28500 56.863 102 44 0 10 111 54 155 1.79e-35 124
MS.gene94227.t1 AT4G00210 44.604 139 76 1 1 138 1 139 2.89e-35 123
MS.gene94227.t1 AT3G03760 52.427 103 48 1 10 111 50 152 3.04e-35 124
MS.gene94227.t1 AT2G40470 68.750 80 25 0 12 91 21 100 5.11e-35 122
MS.gene94227.t1 AT2G42430 43.919 148 80 2 2 146 6 153 1.04e-34 122
MS.gene94227.t1 AT2G40470 68.750 80 25 0 12 91 46 125 1.18e-34 122
MS.gene94227.t1 AT4G00220 56.000 100 43 1 14 112 20 119 1.73e-34 121
MS.gene94227.t1 AT2G45420 53.000 100 46 1 14 112 40 139 6.23e-34 120
MS.gene94227.t1 AT2G42440 46.552 116 61 1 8 122 4 119 3.91e-33 118
MS.gene94227.t1 AT3G58190 48.673 113 57 1 12 123 12 124 5.86e-33 117
MS.gene94227.t1 AT3G26620 45.000 120 62 1 12 131 6 121 8.98e-33 114
MS.gene94227.t1 AT3G26660 45.690 116 62 1 12 127 6 120 1.14e-32 113
MS.gene94227.t1 AT5G06080 47.115 104 54 1 9 111 5 108 2.80e-31 111
MS.gene94227.t1 AT2G45410 51.000 100 48 1 14 112 19 118 5.91e-31 111
MS.gene94227.t1 AT2G31310 44.340 106 58 1 9 113 5 110 3.76e-30 109
MS.gene94227.t1 AT5G35900 38.686 137 83 1 8 143 2 138 8.79e-28 103
MS.gene94227.t1 AT3G50510 40.000 135 78 2 7 140 8 140 3.39e-27 102
MS.gene94227.t1 AT3G50510 40.000 135 78 2 7 140 8 140 3.39e-27 102
MS.gene94227.t1 AT3G13850 38.235 102 63 0 11 112 36 137 7.79e-24 94.7
MS.gene94227.t1 AT1G72980 38.739 111 68 0 8 118 10 120 5.37e-22 89.0
MS.gene94227.t1 AT3G47870 41.379 87 51 0 8 94 33 119 5.46e-20 85.5
MS.gene94227.t1 AT1G06280 46.154 78 42 0 11 88 24 101 3.60e-18 78.6
MS.gene94227.t1 AT4G22700 37.069 116 69 3 8 121 2 115 1.12e-17 77.0
MS.gene94227.t1 AT2G19820 35.165 91 57 2 12 101 13 102 3.33e-12 60.8
MS.gene94227.t1 AT5G15060 38.158 76 43 2 10 82 16 90 4.89e-12 60.8

Find 25 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGAAAGTGCACAAAGTATT+TGG 0.201773 5.4:-65756388 MS.gene94227:CDS
AGGCTTAGAGATCCTGTTTA+TGG 0.242904 5.4:-65756277 MS.gene94227:CDS
GGAAGAAACTGTGAATTCTT+TGG 0.269255 5.4:-65756317 MS.gene94227:CDS
TATGGCGCAAATATACAATT+TGG 0.301099 5.4:+65756439 None:intergenic
ATTTGAATTCATCATATCAT+TGG 0.327427 5.4:+65756126 None:intergenic
CACTCATCGGAGCGAAAGTA+TGG 0.344487 5.4:+65756421 None:intergenic
CGCGAATTTCTTGCACTCAT+CGG 0.347852 5.4:+65756408 None:intergenic
GTTTCTTCCCTTTGTTCCTC+TGG 0.351316 5.4:+65756331 None:intergenic
TAACAGCATTAAAATATATA+AGG 0.400998 5.4:+65755983 None:intergenic
GAAAAGGAAGAAGTTGAAGT+TGG 0.404016 5.4:+65756148 None:intergenic
GCACCTATGCATCCATAAAC+AGG 0.435136 5.4:+65756265 None:intergenic
TGCCATTGCTAAGGATCGTC+TGG 0.450338 5.4:-65756197 MS.gene94227:CDS
ATTCATCATATCATTGGAAA+AGG 0.457173 5.4:+65756132 None:intergenic
CGAAGTTCCAGAGGAACAAA+GGG 0.517072 5.4:-65756338 MS.gene94227:CDS
AAATTTGCAGGCTGCACAAG+AGG 0.545401 5.4:+65756480 None:intergenic
GCATGATCTTGCCATTGCTA+AGG 0.551324 5.4:-65756206 MS.gene94227:CDS
GATCCTGTTTATGGATGCAT+AGG 0.564582 5.4:-65756268 MS.gene94227:CDS
GCACAAGAGGAACTTGACCT+TGG 0.570179 5.4:+65756493 None:intergenic
TCGAAGTTCCAGAGGAACAA+AGG 0.580233 5.4:-65756339 MS.gene94227:CDS
TGGCTTATGAAGCTGATGCA+AGG 0.584407 5.4:-65756297 MS.gene94227:CDS
CACTCTAGAGAAATCATGAA+GGG 0.616662 5.4:-65756523 None:intergenic
AGCCAGACGATCCTTAGCAA+TGG 0.638509 5.4:+65756195 None:intergenic
TCATGAAGGGTTATGAACCA+AGG 0.655644 5.4:-65756510 MS.gene94227:CDS
GATTCTGATCGAAGTTCCAG+AGG 0.676488 5.4:-65756347 MS.gene94227:CDS
TCGTCTGGCTCGTTGCGCTG+CGG 0.690033 5.4:-65756182 MS.gene94227:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGAATTCATCATATCAT+TGG + chr5.4:65756376-65756395 None:intergenic 20.0%
! ATTCATCATATCATTGGAAA+AGG + chr5.4:65756370-65756389 None:intergenic 25.0%
TATGGCGCAAATATACAATT+TGG + chr5.4:65756063-65756082 None:intergenic 30.0%
!! TCTTCTTTTGAGAAATTTGC+AGG + chr5.4:65756034-65756053 None:intergenic 30.0%
CAAGTGAAAAACTCAGGAAA+AGG + chr5.4:65756411-65756430 None:intergenic 35.0%
GAAAAGGAAGAAGTTGAAGT+TGG + chr5.4:65756354-65756373 None:intergenic 35.0%
GGAAGAAACTGTGAATTCTT+TGG - chr5.4:65756182-65756201 MS.gene94227:CDS 35.0%
AGGCTTAGAGATCCTGTTTA+TGG - chr5.4:65756222-65756241 MS.gene94227:CDS 40.0%
GATCCTGTTTATGGATGCAT+AGG - chr5.4:65756231-65756250 MS.gene94227:CDS 40.0%
GTGAAAAACTCAGGAAAAGG+TGG + chr5.4:65756408-65756427 None:intergenic 40.0%
TCACTGCAAGTGAAAAACTC+AGG + chr5.4:65756417-65756436 None:intergenic 40.0%
! AGCACTTTTGCAAAGGAAGA+TGG - chr5.4:65756260-65756279 MS.gene94227:CDS 40.0%
! GCGAAAGTGCACAAAGTATT+TGG - chr5.4:65756111-65756130 MS.gene94227:CDS 40.0%
! GTGCTATAGCACTTTTGCAA+AGG - chr5.4:65756253-65756272 MS.gene94227:CDS 40.0%
AAATTTGCAGGCTGCACAAG+AGG + chr5.4:65756022-65756041 None:intergenic 45.0%
CGAAGTTCCAGAGGAACAAA+GGG - chr5.4:65756161-65756180 MS.gene94227:CDS 45.0%
CGCGAATTTCTTGCACTCAT+CGG + chr5.4:65756094-65756113 None:intergenic 45.0%
GATTCTGATCGAAGTTCCAG+AGG - chr5.4:65756152-65756171 MS.gene94227:CDS 45.0%
GCACCTATGCATCCATAAAC+AGG + chr5.4:65756237-65756256 None:intergenic 45.0%
GCATGATCTTGCCATTGCTA+AGG - chr5.4:65756293-65756312 MS.gene94227:CDS 45.0%
GTTTCTTCCCTTTGTTCCTC+TGG + chr5.4:65756171-65756190 None:intergenic 45.0%
TCGAAGTTCCAGAGGAACAA+AGG - chr5.4:65756160-65756179 MS.gene94227:CDS 45.0%
TGGCTTATGAAGCTGATGCA+AGG - chr5.4:65756202-65756221 MS.gene94227:CDS 45.0%
AGCCAGACGATCCTTAGCAA+TGG + chr5.4:65756307-65756326 None:intergenic 50.0%
CACTCATCGGAGCGAAAGTA+TGG + chr5.4:65756081-65756100 None:intergenic 50.0%
GCACAAGAGGAACTTGACCT+TGG + chr5.4:65756009-65756028 None:intergenic 50.0%
TGCCATTGCTAAGGATCGTC+TGG - chr5.4:65756302-65756321 MS.gene94227:CDS 50.0%
! TCGTCTGGCTCGTTGCGCTG+CGG - chr5.4:65756317-65756336 MS.gene94227:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 65755991 65756530 65755991 ID=MS.gene94227
chr5.4 mRNA 65755991 65756530 65755991 ID=MS.gene94227.t1;Parent=MS.gene94227
chr5.4 exon 65755991 65756530 65755991 ID=MS.gene94227.t1.exon1;Parent=MS.gene94227.t1
chr5.4 CDS 65755991 65756530 65755991 ID=cds.MS.gene94227.t1;Parent=MS.gene94227.t1
Gene Sequence

>MS.gene94227

ATGAAGGGTTATGAACCAAGGTCAAGTTCCTCTTGTGCAGCCTGCAAATTTCTCAAAAGAAGATGCATACCAAATTGTATATTTGCGCCATACTTTCGCTCCGATGAGTGCAAGAAATTCGCGAAAGTGCACAAAGTATTTGGAGCAAGCAATGTGAGCAAGATTCTGATCGAAGTTCCAGAGGAACAAAGGGAAGAAACTGTGAATTCTTTGGCTTATGAAGCTGATGCAAGGCTTAGAGATCCTGTTTATGGATGCATAGGTGCTATAGCACTTTTGCAAAGGAAGATGGTTGAACTTCAGCATGATCTTGCCATTGCTAAGGATCGTCTGGCTCGTTGCGCTGCGGCTGCTGCTACTCCAACTTCAACTTCTTCCTTTTCCAATGATATGATGAATTCAAATGTTAGTTTGCCACCTTTTCCTGAGTTTTTCACTTGCAGTGACTTCAGTGATAATTTAAGTCACAGTTCTTCGTCTCAATCGTTTAGTCGAAATGAAACTGTCGATGATTTTGTTCAAATTCCTTATATATTTTAA

Protein sequence

>MS.gene94227.t1

MKGYEPRSSSSCAACKFLKRRCIPNCIFAPYFRSDECKKFAKVHKVFGASNVSKILIEVPEEQREETVNSLAYEADARLRDPVYGCIGAIALLQRKMVELQHDLAIAKDRLARCAAAAATPTSTSSFSNDMMNSNVSLPPFPEFFTCSDFSDNLSHSSSSQSFSRNETVDDFVQIPYIF