Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94256.t1 | XP_003623166.1 | 96.6 | 117 | 4 | 0 | 1 | 117 | 1 | 117 | 5.30E-58 | 233.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94256.t1 | G7L5L7 | 96.6 | 117 | 4 | 0 | 1 | 117 | 1 | 117 | 3.8e-58 | 233.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051552 | MS.gene94256 | 0.801126 | 1.04E-48 | -1.69E-46 |
| MS.gene055157 | MS.gene94256 | 0.82356 | 1.30E-53 | -1.69E-46 |
| MS.gene055158 | MS.gene94256 | 0.858595 | 7.40E-63 | -1.69E-46 |
| MS.gene055159 | MS.gene94256 | 0.890699 | 7.78E-74 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94256.t1 | MTR_7g065210 | 96.581 | 117 | 4 | 0 | 1 | 117 | 1 | 117 | 2.97e-81 | 233 |
| MS.gene94256.t1 | MTR_7g065160 | 87.179 | 117 | 15 | 0 | 1 | 117 | 1 | 117 | 7.93e-74 | 215 |
| MS.gene94256.t1 | MTR_8g070040 | 50.505 | 99 | 47 | 2 | 20 | 117 | 23 | 120 | 3.47e-25 | 92.0 |
| MS.gene94256.t1 | MTR_8g070015 | 46.739 | 92 | 47 | 2 | 20 | 110 | 23 | 113 | 1.68e-19 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94256.t1 | AT3G53980 | 67.368 | 95 | 30 | 1 | 23 | 117 | 21 | 114 | 6.12e-44 | 139 |
| MS.gene94256.t1 | AT3G53980 | 67.368 | 95 | 30 | 1 | 23 | 117 | 21 | 114 | 6.12e-44 | 139 |
| MS.gene94256.t1 | AT5G05960 | 57.895 | 114 | 41 | 2 | 4 | 117 | 10 | 116 | 7.21e-42 | 134 |
| MS.gene94256.t1 | AT2G37870 | 44.444 | 108 | 58 | 2 | 11 | 117 | 9 | 115 | 3.14e-25 | 92.0 |
Find 22 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAATGCTTCCATATC+TGG | 0.265044 | 7.3:-40716179 | None:intergenic |
| TAGCAAAGTTACAGCGTTTA+GGG | 0.295883 | 7.3:-40716364 | None:intergenic |
| TTAGCAAAGTTACAGCGTTT+AGG | 0.355252 | 7.3:-40716365 | None:intergenic |
| CGTCCGCACTTATAACCAAT+GGG | 0.440432 | 7.3:-40716392 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7.3:+40716314 | MS.gene94256:CDS |
| AAGGGGTACTACACCAGATA+TGG | 0.470603 | 7.3:+40716166 | MS.gene94256:CDS |
| GGATAGTAACAGCAATTTGA+GGG | 0.472152 | 7.3:-40716343 | None:intergenic |
| ATGAAGATGCTGTGCCTAGT+TGG | 0.489536 | 7.3:+40716089 | MS.gene94256:CDS |
| GGGATAGTAACAGCAATTTG+AGG | 0.508534 | 7.3:-40716344 | None:intergenic |
| ACGTCCGCACTTATAACCAA+TGG | 0.512832 | 7.3:-40716393 | None:intergenic |
| GTTGAAAGTGCAGGTGAATG+TGG | 0.515102 | 7.3:+40716143 | MS.gene94256:CDS |
| AACTTTGCTAATCACCCCAT+TGG | 0.522574 | 7.3:+40716377 | MS.gene94256:CDS |
| TCTTGTGCTGCACTTGCACA+AGG | 0.541464 | 7.3:-40716206 | None:intergenic |
| AAAGTGCAGGTGAATGTGGA+AGG | 0.549386 | 7.3:+40716147 | MS.gene94256:CDS |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7.3:-40716287 | None:intergenic |
| ATTCATAGAGTTGAAAGTGC+AGG | 0.591870 | 7.3:+40716134 | MS.gene94256:CDS |
| TGTGCTCAGACTAAGAAACT+TGG | 0.591888 | 7.3:+40716257 | MS.gene94256:CDS |
| CAAGAACTACAAAACCAACT+AGG | 0.604153 | 7.3:-40716103 | None:intergenic |
| AGTGCAGGTGAATGTGGAAG+GGG | 0.630405 | 7.3:+40716149 | MS.gene94256:CDS |
| CACCCCATTGGTTATAAGTG+CGG | 0.632889 | 7.3:+40716389 | MS.gene94256:CDS |
| AAGTGCAGGTGAATGTGGAA+GGG | 0.641454 | 7.3:+40716148 | MS.gene94256:CDS |
| GTCCGCACTTATAACCAATG+GGG | 0.738329 | 7.3:-40716391 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGAGAAATATATTAATAT+AGG | + | chr7.3:40716597-40716616 | MS.gene94256:intron | 10.0% |
| !! | TATATTAATATAGGAAAAAA+AGG | + | chr7.3:40716606-40716625 | MS.gene94256:intron | 10.0% |
| !!! | AATTTTGTATTTTTTTTCTT+AGG | - | chr7.3:40716683-40716702 | None:intergenic | 10.0% |
| !!! | ATTTTGTATTTTTTTTCTTA+GGG | - | chr7.3:40716682-40716701 | None:intergenic | 10.0% |
| !! | AAGAATAAATATAAACGAAA+TGG | - | chr7.3:40716472-40716491 | None:intergenic | 15.0% |
| !!! | TTTCATTGCATTATTTTTTT+TGG | - | chr7.3:40716968-40716987 | None:intergenic | 15.0% |
| !!! | TTTTGTAGTTTTTTTTTCTT+AGG | + | chr7.3:40716892-40716911 | MS.gene94256:intron | 15.0% |
| !!! | TTTTGTATTTTTTTTCTTAG+GGG | - | chr7.3:40716681-40716700 | None:intergenic | 15.0% |
| !! | AACAACAAAAAAATGTGAAA+CGG | + | chr7.3:40716988-40717007 | MS.gene94256:intron | 20.0% |
| !!! | CTATTTGTTAATTTTCTTGA+TGG | + | chr7.3:40717050-40717069 | MS.gene94256:intron | 20.0% |
| !!! | TTTGTATTTTTTTTCTTAGG+GGG | - | chr7.3:40716680-40716699 | None:intergenic | 20.0% |
| GACAACTTAAAAGAAACTGA+GGG | - | chr7.3:40716434-40716453 | None:intergenic | 30.0% | |
| TCATGCAAGTGCAAAAAAAT+TGG | - | chr7.3:40716748-40716767 | None:intergenic | 30.0% | |
| AAAAAAATAACACGTGCACG+TGG | + | chr7.3:40716779-40716798 | MS.gene94256:intron | 35.0% | |
| AAAACTGCACAAAGACAACT+TGG | - | chr7.3:40716290-40716309 | None:intergenic | 35.0% | |
| AGCTTAAATGCTTCCATATC+TGG | - | chr7.3:40716182-40716201 | None:intergenic | 35.0% | |
| CAAGAACTACAAAACCAACT+AGG | - | chr7.3:40716106-40716125 | None:intergenic | 35.0% | |
| CGACAACTTAAAAGAAACTG+AGG | - | chr7.3:40716435-40716454 | None:intergenic | 35.0% | |
| CTAAAATCACAACTTCGTGA+AGG | - | chr7.3:40716657-40716676 | None:intergenic | 35.0% | |
| GGATAGTAACAGCAATTTGA+GGG | - | chr7.3:40716346-40716365 | None:intergenic | 35.0% | |
| TAGCAAAGTTACAGCGTTTA+GGG | - | chr7.3:40716367-40716386 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | + | chr7.3:40716314-40716333 | MS.gene94256:CDS | 35.0% | |
| TGCTACAGTTGCAAAACTTA+GGG | - | chr7.3:40716709-40716728 | None:intergenic | 35.0% | |
| TTAGCAAAGTTACAGCGTTT+AGG | - | chr7.3:40716368-40716387 | None:intergenic | 35.0% | |
| ! | AAGTGCAAAAAAATTGGCCT+TGG | - | chr7.3:40716742-40716761 | None:intergenic | 35.0% |
| ! | AATCGCAACTTTGGAAAGTT+AGG | + | chr7.3:40716922-40716941 | MS.gene94256:intron | 35.0% |
| ! | ACTTTCCAAAGTTGCGATTT+TGG | - | chr7.3:40716921-40716940 | None:intergenic | 35.0% |
| ! | ATCGCAACTTTGGAAAGTTA+GGG | + | chr7.3:40716923-40716942 | MS.gene94256:intron | 35.0% |
| ! | ATTCATAGAGTTGAAAGTGC+AGG | + | chr7.3:40716134-40716153 | MS.gene94256:CDS | 35.0% |
| ! | GTTTTGTAGTTCTTGTTGCA+GGG | + | chr7.3:40716111-40716130 | MS.gene94256:CDS | 35.0% |
| ! | TTTTGCAACTGTAGCAGTTT+TGG | + | chr7.3:40716713-40716732 | MS.gene94256:intron | 35.0% |
| !! | AGTGCAAAAAAATTGGCCTT+GGG | - | chr7.3:40716741-40716760 | None:intergenic | 35.0% |
| AACTTTGCTAATCACCCCAT+TGG | + | chr7.3:40716377-40716396 | MS.gene94256:CDS | 40.0% | |
| AGCGTAAACCGAGTCAAAAT+TGG | + | chr7.3:40716825-40716844 | MS.gene94256:intron | 40.0% | |
| ATCACAACTTCGTGAAGGTT+AGG | - | chr7.3:40716652-40716671 | None:intergenic | 40.0% | |
| CTACAGTTGCAAAACTTAGG+GGG | - | chr7.3:40716707-40716726 | None:intergenic | 40.0% | |
| CTGCTACAGTTGCAAAACTT+AGG | - | chr7.3:40716710-40716729 | None:intergenic | 40.0% | |
| GCTACAGTTGCAAAACTTAG+GGG | - | chr7.3:40716708-40716727 | None:intergenic | 40.0% | |
| GGGATAGTAACAGCAATTTG+AGG | - | chr7.3:40716347-40716366 | None:intergenic | 40.0% | |
| TCACAACTTCGTGAAGGTTA+GGG | - | chr7.3:40716651-40716670 | None:intergenic | 40.0% | |
| TGTGCTCAGACTAAGAAACT+TGG | + | chr7.3:40716257-40716276 | MS.gene94256:CDS | 40.0% | |
| !! | GGTTTTGTAGTTCTTGTTGC+AGG | + | chr7.3:40716110-40716129 | MS.gene94256:CDS | 40.0% |
| !! | GTGCAAAAAAATTGGCCTTG+GGG | - | chr7.3:40716740-40716759 | None:intergenic | 40.0% |
| !! | TGCAAAAAAATTGGCCTTGG+GGG | - | chr7.3:40716739-40716758 | None:intergenic | 40.0% |
| AAAGTGCAGGTGAATGTGGA+AGG | + | chr7.3:40716147-40716166 | MS.gene94256:CDS | 45.0% | |
| AAGTGCAGGTGAATGTGGAA+GGG | + | chr7.3:40716148-40716167 | MS.gene94256:CDS | 45.0% | |
| ACAACTTCGTGAAGGTTAGG+GGG | - | chr7.3:40716649-40716668 | None:intergenic | 45.0% | |
| ACGTCCGCACTTATAACCAA+TGG | - | chr7.3:40716396-40716415 | None:intergenic | 45.0% | |
| ATGAAGATGCTGTGCCTAGT+TGG | + | chr7.3:40716089-40716108 | MS.gene94256:CDS | 45.0% | |
| CACAACTTCGTGAAGGTTAG+GGG | - | chr7.3:40716650-40716669 | None:intergenic | 45.0% | |
| CGTCCGCACTTATAACCAAT+GGG | - | chr7.3:40716395-40716414 | None:intergenic | 45.0% | |
| GGAAGCCAAAATCGCAACTT+TGG | + | chr7.3:40716913-40716932 | MS.gene94256:intron | 45.0% | |
| GTCCGCACTTATAACCAATG+GGG | - | chr7.3:40716394-40716413 | None:intergenic | 45.0% | |
| TCACCGGTGCAAAACTTAGA+GGG | + | chr7.3:40716866-40716885 | MS.gene94256:intron | 45.0% | |
| TGCACGTGGCATCTCATATA+GGG | + | chr7.3:40716793-40716812 | MS.gene94256:intron | 45.0% | |
| ! | AACCGAGTCAAAATTGGCCT+TGG | + | chr7.3:40716831-40716850 | MS.gene94256:intron | 45.0% |
| ! | CACCCCATTGGTTATAAGTG+CGG | + | chr7.3:40716389-40716408 | MS.gene94256:CDS | 45.0% |
| ! | GTTGAAAGTGCAGGTGAATG+TGG | + | chr7.3:40716143-40716162 | MS.gene94256:CDS | 45.0% |
| !! | AAGGGGTACTACACCAGATA+TGG | + | chr7.3:40716166-40716185 | MS.gene94256:CDS | 45.0% |
| !! | ACCGAGTCAAAATTGGCCTT+GGG | + | chr7.3:40716832-40716851 | MS.gene94256:intron | 45.0% |
| !! | TTTTGCACCGGTGAGAGTTT+TGG | - | chr7.3:40716860-40716879 | None:intergenic | 45.0% |
| AGTGCAGGTGAATGTGGAAG+GGG | + | chr7.3:40716149-40716168 | MS.gene94256:CDS | 50.0% | |
| CTCACCGGTGCAAAACTTAG+AGG | + | chr7.3:40716865-40716884 | MS.gene94256:intron | 50.0% | |
| GTGCACGTGGCATCTCATAT+AGG | + | chr7.3:40716792-40716811 | MS.gene94256:intron | 50.0% | |
| TCTTGTGCTGCACTTGCACA+AGG | - | chr7.3:40716209-40716228 | None:intergenic | 50.0% | |
| !! | CCGAGTCAAAATTGGCCTTG+GGG | + | chr7.3:40716833-40716852 | MS.gene94256:intron | 50.0% |
| !! | CGAGTCAAAATTGGCCTTGG+GGG | + | chr7.3:40716834-40716853 | MS.gene94256:intron | 50.0% |
| !! | TGACTCGGTTTACGCTGATG+TGG | - | chr7.3:40716821-40716840 | None:intergenic | 50.0% |
| !!! | CCCCAAGGCCAATTTTGACT+CGG | - | chr7.3:40716836-40716855 | None:intergenic | 50.0% |
| CCGGTGCAAAACTTAGAGGG+CGG | + | chr7.3:40716869-40716888 | MS.gene94256:intron | 55.0% | |
| TTGGGGGCCAAAACTCTCAC+CGG | + | chr7.3:40716850-40716869 | MS.gene94256:intron | 55.0% | |
| ! | CCGCCCTCTAAGTTTTGCAC+CGG | - | chr7.3:40716872-40716891 | None:intergenic | 55.0% |
| !! | TGTAGCAGTTTTGGCCCCCA+AGG | + | chr7.3:40716722-40716741 | MS.gene94256:intron | 55.0% |
| !! | GGTGAGAGTTTTGGCCCCCA+AGG | - | chr7.3:40716851-40716870 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 40716077 | 40717130 | 40716077 | ID=MS.gene94256 |
| chr7.3 | mRNA | 40716077 | 40717130 | 40716077 | ID=MS.gene94256.t1;Parent=MS.gene94256 |
| chr7.3 | exon | 40716077 | 40716413 | 40716077 | ID=MS.gene94256.t1.exon1;Parent=MS.gene94256.t1 |
| chr7.3 | CDS | 40716077 | 40716413 | 40716077 | ID=cds.MS.gene94256.t1;Parent=MS.gene94256.t1 |
| chr7.3 | exon | 40717114 | 40717130 | 40717114 | ID=MS.gene94256.t1.exon2;Parent=MS.gene94256.t1 |
| chr7.3 | CDS | 40717114 | 40717130 | 40717114 | ID=cds.MS.gene94256.t1;Parent=MS.gene94256.t1 |
| Gene Sequence |
| Protein sequence |
