AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94649

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94649.t1 MTR_2g084715 98.457 324 5 0 1 324 1 324 0.0 655
MS.gene94649.t1 MTR_7g103630 64.537 313 82 6 1 292 1 305 7.15e-133 381
MS.gene94649.t1 MTR_1g035130 65.614 285 91 1 1 285 1 278 8.92e-125 358
MS.gene94649.t1 MTR_2g005450 40.000 170 97 2 9 175 184 351 9.02e-38 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94649.t1 AT5G42820 69.338 287 79 3 1 284 1 281 1.42e-131 376
MS.gene94649.t1 AT5G42820 69.338 287 79 3 1 284 1 281 1.42e-131 376
MS.gene94649.t1 AT1G27650 66.084 286 89 2 1 279 1 285 4.12e-124 358
MS.gene94649.t1 AT1G27650 60.593 236 85 2 51 279 1 235 5.81e-85 256
MS.gene94649.t1 AT1G10320 41.765 170 94 3 9 175 237 404 5.48e-39 146
MS.gene94649.t1 AT1G10320 41.765 170 94 3 9 175 228 395 5.86e-39 146
MS.gene94649.t1 AT3G44785 85.185 54 8 0 1 54 1 54 6.38e-29 106

Find 87 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGAGAAATCAACAATAAT+TGG 0.248460 2.4:+14539069 None:intergenic
TTTGATGAATTGTCTAAGTA+TGG 0.330686 2.4:-14540324 MS.gene94649:CDS
GTGCTCGTTATGGCCATTAC+TGG 0.331071 2.4:+14538503 None:intergenic
GAATTGAGGCGTCAATTGTT+TGG 0.359369 2.4:-14538855 MS.gene94649:CDS
TATCGATCCAAGGAAGATTC+AGG 0.373191 2.4:-14540376 MS.gene94649:CDS
CGAGAATTGAGCAGTGGAAC+AGG 0.379353 2.4:-14538572 MS.gene94649:CDS
TCTGTATTAACCTTACTTGT+GGG 0.387455 2.4:+14538531 None:intergenic
AAATGATCCTGAATCTTCCT+TGG 0.410163 2.4:+14540369 None:intergenic
TGCTCCTACTGTGCCTTTCG+TGG 0.422489 2.4:+14538826 None:intergenic
ACACCAGGAGTAATCATATC+AGG 0.425297 2.4:+14540417 None:intergenic
ACTCCTGGTGTTGATGTTCA+TGG 0.428345 2.4:-14540405 MS.gene94649:CDS
CTCTGTATTAACCTTACTTG+TGG 0.432993 2.4:+14538530 None:intergenic
GGAGGAATCGTAGTCCAGTC+AGG 0.441211 2.4:-14538617 MS.gene94649:CDS
CTCCTTCCCCTTCTCCGTCC+AGG 0.455533 2.4:+14538669 None:intergenic
CTACGATTCCTCCTTCCAAC+AGG 0.474480 2.4:+14538627 None:intergenic
TGTTGATTTCTCGCCGGTGA+CGG 0.475826 2.4:-14539061 MS.gene94649:CDS
TCATAGCTTCGATGTCTGTA+AGG 0.480825 2.4:+14538792 None:intergenic
TCATGAGAATCAGCAGCAAC+GGG 0.483117 2.4:-14538460 MS.gene94649:CDS
TTCAAGTCCTGGACGGAGAA+GGG 0.489573 2.4:-14538676 MS.gene94649:CDS
TCCTCGCTACCATCCCTGAC+TGG 0.490385 2.4:+14538603 None:intergenic
GGCTTATGCTTGGCTTAGTG+TGG 0.492967 2.4:+14540472 None:intergenic
ACGAAAGGCACAGTAGGAGC+AGG 0.495855 2.4:-14538824 MS.gene94649:CDS
TTGAGCAGTGGAACAGGGAA+AGG 0.500758 2.4:-14538566 MS.gene94649:CDS
GCTCCGATCTTGAAGTAGAA+TGG 0.500982 2.4:+14540525 None:intergenic
ATGATGACAGAGACAGAGAC+AGG 0.502116 2.4:-14538737 MS.gene94649:CDS
CTTCAAGTCCTGGACGGAGA+AGG 0.506232 2.4:-14538677 MS.gene94649:CDS
AGCAGCAACGGGAATCTCCT+CGG 0.506550 2.4:-14538449 MS.gene94649:CDS
TGCACTTGAAAAGAATTAGC+AGG 0.510770 2.4:-14538969 MS.gene94649:intron
AGAGAAGGAACCCACAAGTA+AGG 0.511488 2.4:-14538541 MS.gene94649:CDS
GCAGCAACGGGAATCTCCTC+GGG 0.512228 2.4:-14538448 MS.gene94649:CDS
GAACAGGGAAAGGGAAGAGA+AGG 0.512985 2.4:-14538556 MS.gene94649:CDS
GCCTCACGAAAGTCCGTCAC+CGG 0.516100 2.4:+14539048 None:intergenic
GGCTACCTGCAGGCAGTATG+AGG 0.520145 2.4:-14539028 MS.gene94649:CDS
TGTTGGAGAGGAGGATCGTA+GGG 0.521994 2.4:+14540451 None:intergenic
GCATTGTGTTCAGGGAATTG+AGG 0.523927 2.4:-14538869 MS.gene94649:intron
TGAATCTTCCTTGGATCGAT+AGG 0.523928 2.4:+14540378 None:intergenic
GGCACAGTAGGAGCAGGAGC+AGG 0.549754 2.4:-14538818 MS.gene94649:CDS
ATCATGAGAATCAGCAGCAA+CGG 0.551987 2.4:-14538461 MS.gene94649:CDS
TGTGTATGTTCAGTTTAGAG+AGG 0.556449 2.4:-14539989 MS.gene94649:CDS
AATGCTGTTAAGAATCTCAC+TGG 0.558427 2.4:-14539952 MS.gene94649:CDS
AGTCGCAGTCGAAGTCCTGT+TGG 0.558621 2.4:-14538642 MS.gene94649:CDS
CGTCCTGATATGATTACTCC+TGG 0.564634 2.4:-14540420 MS.gene94649:CDS
ATCAGGACGCTGGTACATGT+TGG 0.566009 2.4:+14540434 None:intergenic
ATCATCACGACAGTGATAGC+AGG 0.569349 2.4:-14538710 MS.gene94649:CDS
AGGATCGTAGGGCTTATGCT+TGG 0.570022 2.4:+14540462 None:intergenic
AGACGCAGGACTTCAAGTCC+TGG 0.570258 2.4:-14538687 MS.gene94649:CDS
TGTCCATTCTACTTCAAGAT+CGG 0.570630 2.4:-14540528 MS.gene94649:CDS
TCCAGTCAGGGATGGTAGCG+AGG 0.571668 2.4:-14538604 MS.gene94649:CDS
ATTCTCCTCATACTGCCTGC+AGG 0.573391 2.4:+14539023 None:intergenic
GCAACGGGAATCTCCTCGGG+AGG 0.573485 2.4:-14538445 MS.gene94649:CDS
ATGTTGGAGAGGAGGATCGT+AGG 0.576366 2.4:+14540450 None:intergenic
ACAGGACTTCGACTGCGACT+AGG 0.576849 2.4:+14538645 None:intergenic
TCAAGTCCTGGACGGAGAAG+GGG 0.603608 2.4:-14538675 MS.gene94649:CDS
TGAGCAGTGGAACAGGGAAA+GGG 0.605591 2.4:-14538565 MS.gene94649:CDS
TGTTTGGAAAGCGCCACGAA+AGG 0.607382 2.4:-14538839 MS.gene94649:CDS
TGATAATCTCGCTGATCACA+TGG 0.609267 2.4:-14540277 MS.gene94649:intron
GCAGTCGAAGTCCTGTTGGA+AGG 0.609805 2.4:-14538638 MS.gene94649:CDS
AATTATTGTTGATTTCTCGC+CGG 0.610789 2.4:-14539067 MS.gene94649:CDS
ACTTTCGTGAGGCTACCTGC+AGG 0.614398 2.4:-14539038 MS.gene94649:CDS
AATACAGAGGAAACCAGTAA+TGG 0.620341 2.4:-14538516 MS.gene94649:CDS
GCCGGTGACGGACTTTCGTG+AGG 0.622851 2.4:-14539049 MS.gene94649:CDS
AGCGCCACGAAAGGCACAGT+AGG 0.625915 2.4:-14538830 MS.gene94649:CDS
TTACAGACATCGAAGCTATG+AGG 0.626441 2.4:-14538790 MS.gene94649:CDS
GACGCTGGTACATGTTGGAG+AGG 0.626458 2.4:+14540439 None:intergenic
AATCGTAGTCCAGTCAGGGA+TGG 0.626830 2.4:-14538612 MS.gene94649:CDS
GTCGAAGTCCTGTTGGAAGG+AGG 0.630939 2.4:-14538635 MS.gene94649:CDS
GACATCGAAGCTATGAGGAG+AGG 0.631190 2.4:-14538785 MS.gene94649:CDS
ATGGACAACCTATCGATCCA+AGG 0.637915 2.4:-14540386 MS.gene94649:CDS
GCTGGTACATGTTGGAGAGG+AGG 0.641329 2.4:+14540442 None:intergenic
GAGGAATCGTAGTCCAGTCA+GGG 0.642243 2.4:-14538616 MS.gene94649:CDS
GACGTGCGAGAATTGAGCAG+TGG 0.650018 2.4:-14538578 MS.gene94649:CDS
GAGGAGAATACTTGCAACCG+TGG 0.652968 2.4:-14539009 MS.gene94649:CDS
CACAAGTAAGGTTAATACAG+AGG 0.653053 2.4:-14538529 MS.gene94649:CDS
GAGTAATCATATCAGGACGC+TGG 0.656340 2.4:+14540424 None:intergenic
GAGAATTGAGCAGTGGAACA+GGG 0.660915 2.4:-14538571 MS.gene94649:CDS
AAGATCGGAGCATGTCGACA+CGG 0.663894 2.4:-14540513 MS.gene94649:CDS
TGTCCATGAACATCAACACC+AGG 0.664950 2.4:+14540402 None:intergenic
ACAGTGATAGCAGGAGACGC+AGG 0.667375 2.4:-14538701 MS.gene94649:CDS
GTCCTGGACGGAGAAGGGGA+AGG 0.667415 2.4:-14538671 MS.gene94649:CDS
ACAGATCAATATCCCTCCCG+AGG 0.670159 2.4:+14538432 None:intergenic
CAACGGGAATCTCCTCGGGA+GGG 0.671228 2.4:-14538444 MS.gene94649:CDS
TGATGACAGAGACAGAGACA+GGG 0.672868 2.4:-14538736 MS.gene94649:CDS
GCAGGACTTCAAGTCCTGGA+CGG 0.684119 2.4:-14538683 MS.gene94649:CDS
AGTAGAATGGACAGTTAACG+CGG 0.690073 2.4:+14540538 None:intergenic
GAGAATACTTGCAACCGTGG+TGG 0.696644 2.4:-14539006 MS.gene94649:CDS
TTACTGCTACGACTGCGATG+AGG 0.703201 2.4:+14538762 None:intergenic
TGAAGTTGCAATAACCACCA+CGG 0.740505 2.4:+14538992 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTGATTTATATTTTTTTTA+GGG - chr2.4:14538941-14538960 MS.gene94649:intron 10.0%
!!! TTGTGATTTATATTTTTTTT+AGG - chr2.4:14538940-14538959 MS.gene94649:intron 10.0%
!! TGATTTAATTGAATTAATAC+TGG - chr2.4:14538896-14538915 MS.gene94649:intron 15.0%
!!! TTATGATTTTTTGTTTTTTC+AGG - chr2.4:14538997-14539016 MS.gene94649:CDS 15.0%
!! TGTAATGTAATTACATATAC+CGG + chr2.4:14538860-14538879 None:intergenic 20.0%
! AAAAATACCTAAACCACAAA+AGG + chr2.4:14540099-14540118 None:intergenic 25.0%
! AAAATACCTAAACCACAAAA+GGG + chr2.4:14540098-14540117 None:intergenic 25.0%
! ATGTATGTATGTACTTGTAT+AGG - chr2.4:14539248-14539267 MS.gene94649:intron 25.0%
! TACAAAATGTTGTCAAATAG+TGG - chr2.4:14539663-14539682 MS.gene94649:intron 25.0%
! TGTATGTATGTACTTGTATA+GGG - chr2.4:14539249-14539268 MS.gene94649:intron 25.0%
! TTTGATGAATTGTCTAAGTA+TGG - chr2.4:14538692-14538711 MS.gene94649:CDS 25.0%
!! CATTGGTTAAGAAAAAAAAC+TGG + chr2.4:14539549-14539568 None:intergenic 25.0%
!!! GAACATTTAGCTTCAATTTT+CGG - chr2.4:14538443-14538462 MS.gene94649:CDS 25.0%
!!! GATTTTTTGTTTTTTCAGGT+TGG - chr2.4:14539001-14539020 MS.gene94649:CDS 25.0%
!!! GGTATTTTTTAACTAAACGT+TGG - chr2.4:14540110-14540129 MS.gene94649:intron 25.0%
!!! GTTTTAATTCTGTTGTGATT+TGG - chr2.4:14538974-14538993 MS.gene94649:CDS 25.0%
!!! TCATTTTGAAGAGTTTTATG+AGG - chr2.4:14538664-14538683 MS.gene94649:CDS 25.0%
!!! TGTGTTTGTGTGTATTTTTT+GGG - chr2.4:14539207-14539226 MS.gene94649:intron 25.0%
AACAGATAATAGCACGAAAT+TGG + chr2.4:14539864-14539883 None:intergenic 30.0%
ATGATAGTACTATTGCATAG+CGG - chr2.4:14539407-14539426 MS.gene94649:intron 30.0%
CCAAATCCTTTATTATAGAC+GGG - chr2.4:14539835-14539854 MS.gene94649:intron 30.0%
CTAAATCAAATTCTCAACTC+AGG + chr2.4:14539810-14539829 None:intergenic 30.0%
GATATAGTGCTATAGCTTAT+TGG - chr2.4:14539602-14539621 MS.gene94649:intron 30.0%
GCCAAATCCTTTATTATAGA+CGG - chr2.4:14539834-14539853 MS.gene94649:intron 30.0%
TCTGTATTAACCTTACTTGT+GGG + chr2.4:14540488-14540507 None:intergenic 30.0%
TTGTTAAACAGCAGTTATAG+TGG - chr2.4:14539578-14539597 MS.gene94649:intron 30.0%
TTTAACATGCATTGTGTTCA+GGG - chr2.4:14540139-14540158 MS.gene94649:intron 30.0%
! GAGTTGAGAATTTGATTTAG+AGG - chr2.4:14539810-14539829 MS.gene94649:intron 30.0%
!! AATTATTGTTGATTTCTCGC+CGG - chr2.4:14539949-14539968 MS.gene94649:CDS 30.0%
!!! GTGTGTTTGTGTGTATTTTT+TGG - chr2.4:14539206-14539225 MS.gene94649:intron 30.0%
AAATGATCCTGAATCTTCCT+TGG + chr2.4:14538650-14538669 None:intergenic 35.0%
AATACAGAGGAAACCAGTAA+TGG - chr2.4:14540500-14540519 MS.gene94649:CDS 35.0%
AATGCTGTTAAGAATCTCAC+TGG - chr2.4:14539064-14539083 MS.gene94649:CDS 35.0%
CACAAGTAAGGTTAATACAG+AGG - chr2.4:14540487-14540506 MS.gene94649:CDS 35.0%
CCCGTCTATAATAAAGGATT+TGG + chr2.4:14539838-14539857 None:intergenic 35.0%
CTCTGTATTAACCTTACTTG+TGG + chr2.4:14540489-14540508 None:intergenic 35.0%
GCAGGTAATATTTCCAACAT+TGG - chr2.4:14540065-14540084 MS.gene94649:intron 35.0%
GGCGAGAAATCAACAATAAT+TGG + chr2.4:14539950-14539969 None:intergenic 35.0%
GTTTAACATGCATTGTGTTC+AGG - chr2.4:14540138-14540157 MS.gene94649:intron 35.0%
TAGTCACTGTTTCACAATAG+CGG + chr2.4:14539445-14539464 None:intergenic 35.0%
TGCACTTGAAAAGAATTAGC+AGG - chr2.4:14540047-14540066 MS.gene94649:intron 35.0%
TGGATACCCGTCTATAATAA+AGG + chr2.4:14539844-14539863 None:intergenic 35.0%
TGTCCATTCTACTTCAAGAT+CGG - chr2.4:14538488-14538507 MS.gene94649:CDS 35.0%
TGTGGAGGAATTTGAACAAA+CGG - chr2.4:14539485-14539504 MS.gene94649:intron 35.0%
TGTGTATGTTCAGTTTAGAG+AGG - chr2.4:14539027-14539046 MS.gene94649:CDS 35.0%
TTGTCAAATAGTGGCTATAG+CGG - chr2.4:14539672-14539691 MS.gene94649:intron 35.0%
TTTAACAACACTGGTCACAT+TGG + chr2.4:14539566-14539585 None:intergenic 35.0%
! GAACCAGTGTTGTTAAATAG+CGG - chr2.4:14539383-14539402 MS.gene94649:intron 35.0%
! TAACTGCTGTTTAACAACAC+TGG + chr2.4:14539575-14539594 None:intergenic 35.0%
AAAGGGTTACTGACCAATGT+TGG + chr2.4:14540081-14540100 None:intergenic 40.0%
ACACCAGGAGTAATCATATC+AGG + chr2.4:14538602-14538621 None:intergenic 40.0%
AGTAGAATGGACAGTTAACG+CGG + chr2.4:14538481-14538500 None:intergenic 40.0%
ATCATGAGAATCAGCAGCAA+CGG - chr2.4:14540555-14540574 MS.gene94649:CDS 40.0%
ATGCCGCTATTTAACAACAC+TGG + chr2.4:14539389-14539408 None:intergenic 40.0%
CACATCAGTGTTGTCAATTG+CGG + chr2.4:14539525-14539544 None:intergenic 40.0%
TATCGATCCAAGGAAGATTC+AGG - chr2.4:14538640-14538659 MS.gene94649:CDS 40.0%
TCATAGCTTCGATGTCTGTA+AGG + chr2.4:14540227-14540246 None:intergenic 40.0%
TGAAGTTGCAATAACCACCA+CGG + chr2.4:14540027-14540046 None:intergenic 40.0%
TGAATCTTCCTTGGATCGAT+AGG + chr2.4:14538641-14538660 None:intergenic 40.0%
TGATAATCTCGCTGATCACA+TGG - chr2.4:14538739-14538758 MS.gene94649:CDS 40.0%
TGCAAGCATGGAAGAAGAAA+AGG + chr2.4:14539921-14539940 None:intergenic 40.0%
TTACAGACATCGAAGCTATG+AGG - chr2.4:14540226-14540245 MS.gene94649:intron 40.0%
! ATTGGTCAGTAACCCTTTTG+TGG - chr2.4:14540083-14540102 MS.gene94649:intron 40.0%
! CAGTAACCCTTTTGTGGTTT+AGG - chr2.4:14540089-14540108 MS.gene94649:intron 40.0%
! CATTTTGTGAGTGCTCGTTA+TGG + chr2.4:14540526-14540545 None:intergenic 40.0%
! CTCACTGGAAGATTTTATGC+TGG - chr2.4:14539079-14539098 MS.gene94649:intron 40.0%
! GAATTGAGGCGTCAATTGTT+TGG - chr2.4:14540161-14540180 MS.gene94649:intron 40.0%
! TCAGTGATATAGCTCTGTTG+TGG - chr2.4:14539467-14539486 MS.gene94649:intron 40.0%
! TTTTCTTCTTCCATGCTTGC+AGG - chr2.4:14539920-14539939 MS.gene94649:intron 40.0%
AGAGAAGGAACCCACAAGTA+AGG - chr2.4:14540475-14540494 MS.gene94649:CDS 45.0%
ATCATCACGACAGTGATAGC+AGG - chr2.4:14540306-14540325 MS.gene94649:CDS 45.0%
ATGATGACAGAGACAGAGAC+AGG - chr2.4:14540279-14540298 MS.gene94649:CDS 45.0%
ATGGACAACCTATCGATCCA+AGG - chr2.4:14538630-14538649 MS.gene94649:CDS 45.0%
CGTCCTGATATGATTACTCC+TGG - chr2.4:14538596-14538615 MS.gene94649:CDS 45.0%
GAGAATTGAGCAGTGGAACA+GGG - chr2.4:14540445-14540464 MS.gene94649:CDS 45.0%
GAGTAATCATATCAGGACGC+TGG + chr2.4:14538595-14538614 None:intergenic 45.0%
GCATTGTGTTCAGGGAATTG+AGG - chr2.4:14540147-14540166 MS.gene94649:intron 45.0%
GCTCCGATCTTGAAGTAGAA+TGG + chr2.4:14538494-14538513 None:intergenic 45.0%
TCATGAGAATCAGCAGCAAC+GGG - chr2.4:14540556-14540575 MS.gene94649:CDS 45.0%
TGATGACAGAGACAGAGACA+GGG - chr2.4:14540280-14540299 MS.gene94649:CDS 45.0%
TGTCCATGAACATCAACACC+AGG + chr2.4:14538617-14538636 None:intergenic 45.0%
! GCACCTTCAATCAGAAGTGT+CGG + chr2.4:14538805-14538824 None:intergenic 45.0%
! GTGATATAGCTCTGTTGTGG+AGG - chr2.4:14539470-14539489 MS.gene94649:intron 45.0%
!! ACTCCTGGTGTTGATGTTCA+TGG - chr2.4:14538611-14538630 MS.gene94649:CDS 45.0%
AAGATCGGAGCATGTCGACA+CGG - chr2.4:14538503-14538522 MS.gene94649:CDS 50.0%
AATCGTAGTCCAGTCAGGGA+TGG - chr2.4:14540404-14540423 MS.gene94649:CDS 50.0%
AATTGGACGACCTGCAAGCA+TGG + chr2.4:14539933-14539952 None:intergenic 50.0%
AGGATCGTAGGGCTTATGCT+TGG + chr2.4:14538557-14538576 None:intergenic 50.0%
ATCAGGACGCTGGTACATGT+TGG + chr2.4:14538585-14538604 None:intergenic 50.0%
ATGTTGGAGAGGAGGATCGT+AGG + chr2.4:14538569-14538588 None:intergenic 50.0%
ATTCTCCTCATACTGCCTGC+AGG + chr2.4:14539996-14540015 None:intergenic 50.0%
CGAGAATTGAGCAGTGGAAC+AGG - chr2.4:14540444-14540463 MS.gene94649:CDS 50.0%
CTACGATTCCTCCTTCCAAC+AGG + chr2.4:14540392-14540411 None:intergenic 50.0%
GAACAGGGAAAGGGAAGAGA+AGG - chr2.4:14540460-14540479 MS.gene94649:CDS 50.0%
GACATCGAAGCTATGAGGAG+AGG - chr2.4:14540231-14540250 MS.gene94649:intron 50.0%
GAGAATACTTGCAACCGTGG+TGG - chr2.4:14540010-14540029 MS.gene94649:intron 50.0%
GAGGAATCGTAGTCCAGTCA+GGG - chr2.4:14540400-14540419 MS.gene94649:CDS 50.0%
GAGGAGAATACTTGCAACCG+TGG - chr2.4:14540007-14540026 MS.gene94649:intron 50.0%
GGCTTATGCTTGGCTTAGTG+TGG + chr2.4:14538547-14538566 None:intergenic 50.0%
GTGCTCGTTATGGCCATTAC+TGG + chr2.4:14540516-14540535 None:intergenic 50.0%
TGAGCAGTGGAACAGGGAAA+GGG - chr2.4:14540451-14540470 MS.gene94649:CDS 50.0%
TGTTGGAGAGGAGGATCGTA+GGG + chr2.4:14538568-14538587 None:intergenic 50.0%
TTACTGCTACGACTGCGATG+AGG + chr2.4:14540257-14540276 None:intergenic 50.0%
TTCAAGTCCTGGACGGAGAA+GGG - chr2.4:14540340-14540359 MS.gene94649:CDS 50.0%
TTGAGCAGTGGAACAGGGAA+AGG - chr2.4:14540450-14540469 MS.gene94649:CDS 50.0%
! GCACCGACACTTCTGATTGA+AGG - chr2.4:14538799-14538818 MS.gene94649:CDS 50.0%
!! TGTTGATTTCTCGCCGGTGA+CGG - chr2.4:14539955-14539974 MS.gene94649:CDS 50.0%
!! TGTTTGGAAAGCGCCACGAA+AGG - chr2.4:14540177-14540196 MS.gene94649:intron 50.0%
ACAGGACTTCGACTGCGACT+AGG + chr2.4:14540374-14540393 None:intergenic 55.0%
ACAGTGATAGCAGGAGACGC+AGG - chr2.4:14540315-14540334 MS.gene94649:CDS 55.0%
ACTTTCGTGAGGCTACCTGC+AGG - chr2.4:14539978-14539997 MS.gene94649:CDS 55.0%
AGACGCAGGACTTCAAGTCC+TGG - chr2.4:14540329-14540348 MS.gene94649:CDS 55.0%
AGCAGCAACGGGAATCTCCT+CGG - chr2.4:14540567-14540586 MS.gene94649:CDS 55.0%
AGGTGCGTCAGACATCTGTC+TGG - chr2.4:14538819-14538838 MS.gene94649:CDS 55.0%
AGTCGCAGTCGAAGTCCTGT+TGG - chr2.4:14540374-14540393 MS.gene94649:CDS 55.0%
CTGGTACGACATCACGACAC+CGG - chr2.4:14538838-14538857 MS.gene94649:CDS 55.0%
CTTCAAGTCCTGGACGGAGA+AGG - chr2.4:14540339-14540358 MS.gene94649:CDS 55.0%
GACGCTGGTACATGTTGGAG+AGG + chr2.4:14538580-14538599 None:intergenic 55.0%
GACGTGCGAGAATTGAGCAG+TGG - chr2.4:14540438-14540457 MS.gene94649:CDS 55.0%
GCAGGACTTCAAGTCCTGGA+CGG - chr2.4:14540333-14540352 MS.gene94649:CDS 55.0%
GCAGTCGAAGTCCTGTTGGA+AGG - chr2.4:14540378-14540397 MS.gene94649:CDS 55.0%
GCTGGTACATGTTGGAGAGG+AGG + chr2.4:14538577-14538596 None:intergenic 55.0%
GGAGGAATCGTAGTCCAGTC+AGG - chr2.4:14540399-14540418 MS.gene94649:CDS 55.0%
GTCGAAGTCCTGTTGGAAGG+AGG - chr2.4:14540381-14540400 MS.gene94649:CDS 55.0%
TCAAGTCCTGGACGGAGAAG+GGG - chr2.4:14540341-14540360 MS.gene94649:CDS 55.0%
TGCTCCTACTGTGCCTTTCG+TGG + chr2.4:14540193-14540212 None:intergenic 55.0%
! ACGAAAGGCACAGTAGGAGC+AGG - chr2.4:14540192-14540211 MS.gene94649:intron 55.0%
CAACGGGAATCTCCTCGGGA+GGG - chr2.4:14540572-14540591 MS.gene94649:CDS 60.0%
GCAGCAACGGGAATCTCCTC+GGG - chr2.4:14540568-14540587 MS.gene94649:CDS 60.0%
GCCTCACGAAAGTCCGTCAC+CGG + chr2.4:14539971-14539990 None:intergenic 60.0%
GGCTACCTGCAGGCAGTATG+AGG - chr2.4:14539988-14540007 MS.gene94649:CDS 60.0%
TCCTCGCTACCATCCCTGAC+TGG + chr2.4:14540416-14540435 None:intergenic 60.0%
! AGCGCCACGAAAGGCACAGT+AGG - chr2.4:14540186-14540205 MS.gene94649:intron 60.0%
! TCCAGTCAGGGATGGTAGCG+AGG - chr2.4:14540412-14540431 MS.gene94649:CDS 60.0%
CTCCTTCCCCTTCTCCGTCC+AGG + chr2.4:14540350-14540369 None:intergenic 65.0%
GCAACGGGAATCTCCTCGGG+AGG - chr2.4:14540571-14540590 MS.gene94649:CDS 65.0%
GGCACAGTAGGAGCAGGAGC+AGG - chr2.4:14540198-14540217 MS.gene94649:intron 65.0%
GTCCTGGACGGAGAAGGGGA+AGG - chr2.4:14540345-14540364 MS.gene94649:CDS 65.0%
!! GCCGGTGACGGACTTTCGTG+AGG - chr2.4:14539967-14539986 MS.gene94649:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 14538437 14540601 14538437 ID=MS.gene94649
chr2.4 mRNA 14538437 14540601 14538437 ID=MS.gene94649.t1;Parent=MS.gene94649
chr2.4 exon 14540278 14540601 14540278 ID=MS.gene94649.t1.exon1;Parent=MS.gene94649.t1
chr2.4 CDS 14540278 14540601 14540278 ID=cds.MS.gene94649.t1;Parent=MS.gene94649.t1
chr2.4 exon 14539938 14540019 14539938 ID=MS.gene94649.t1.exon2;Parent=MS.gene94649.t1
chr2.4 CDS 14539938 14540019 14539938 ID=cds.MS.gene94649.t1;Parent=MS.gene94649.t1
chr2.4 exon 14538970 14539096 14538970 ID=MS.gene94649.t1.exon3;Parent=MS.gene94649.t1
chr2.4 CDS 14538970 14539096 14538970 ID=cds.MS.gene94649.t1;Parent=MS.gene94649.t1
chr2.4 exon 14538437 14538878 14538437 ID=MS.gene94649.t1.exon4;Parent=MS.gene94649.t1
chr2.4 CDS 14538437 14538878 14538437 ID=cds.MS.gene94649.t1;Parent=MS.gene94649.t1
Gene Sequence

>MS.gene94649

ATGGCGGAACATTTAGCTTCAATTTTCGGCACAGAGAAAGACCGCGTTAACTGTCCATTCTACTTCAAGATCGGAGCATGTCGACACGGCGATCGATGCTCTCGTCTCCACACTAAGCCAAGCATAAGCCCTACGATCCTCCTCTCCAACATGTACCAGCGTCCTGATATGATTACTCCTGGTGTTGATGTTCATGGACAACCTATCGATCCAAGGAAGATTCAGGATCATTTTGAAGAGTTTTATGAGGATTTGTTTGATGAATTGTCTAAGTATGGTGATATTGAAAGTCTTAATGTTTGTGATAATCTCGCTGATCACATGGTACGTAATTCATCTTCATTTCGTTAATTTACTATGTTGCACCGACACTTCTGATTGAAGGTGCGTCAGACATCTGTCTGGTACGACATCACGACACCGGTATATGTAATTACATTACAGTTATATTCAATTATGTGATTTAATTGAATTAATACTGGTGTTGACATGTCAGTGTGTGTTTGTGATTTATATTTTTTTTAGGGTTTTTTATTTGTTTTAATTCTGTTGTGATTTGGTTATGATTTTTTGTTTTTTCAGGTTGGTAATGTGTATGTTCAGTTTAGAGAGGAAGAGCATGCTGCAAATGCTGTTAAGAATCTCACTGGAAGATTTTATGCTGGTGTGTTATCTGATTTATTTGTCATTTATTATTGTTTTTTTTTTTTTATTTCGAATTTAAATGTTGATAATTGATGACTATTAGATACTAGATGTTAGTGACTTAGTGTGTTTGTGTGTATTTTTTGGGTAATGAACTTAATTATGTATGTATGTATGTACTTGTATAGGGATTTAGATTCTATAATGTGAAATGTTCAGTTTAGAATGATAAGTAAATAATCTCAATTGTTGATGAGAAAAACTGTTGATATTGTAACACATTCATGAGTTGCTTTCATGTGAACCAGTGTTGTTAAATAGCGGCATGATAGTACTATTGCATAGCGGAATTTGAGCTATCCGCTATTGTGAAACAGTGACTATATCAGTGATATAGCTCTGTTGTGGAGGAATTTGAACAAACGGCTCTTTTACGTGATCCGCAATTGACAACACTGATGTGACCAGTTTTTTTTCTTAACCAATGTGACCAGTGTTGTTAAACAGCAGTTATAGTGGTGATATAGTGCTATAGCTTATTGGAATTTTATTAAATTGTTGTTCTGCGATACACTGTTGAGTACAAAATGTTGTCAAATAGTGGCTATAGCGGCGTTGTAGCATTGTTGCATTGCATAATCTTAACAAACTGATTAACAACATTGATGTGAACATATTATCAACTCTTGATTTTCTCATCGAAGATCGAGAGATTACTTAGTTTACCTGAGTTGAGAATTTGATTTAGAGGAGCCAAATCCTTTATTATAGACGGGTATCCAATTTCGTGCTATTATCTGTTGAGCTGCAATTTTTTGTGAAGTTTGAATATCAGACCTTTTCTTCTTCCATGCTTGCAGGTCGTCCAATTATTGTTGATTTCTCGCCGGTGACGGACTTTCGTGAGGCTACCTGCAGGCAGTATGAGGAGAATACTTGCAACCGTGGTGGTTATTGCAACTTCATGCACTTGAAAAGAATTAGCAGGTAATATTTCCAACATTGGTCAGTAACCCTTTTGTGGTTTAGGTATTTTTTAACTAAACGTTGGTGATTGTTTAACATGCATTGTGTTCAGGGAATTGAGGCGTCAATTGTTTGGAAAGCGCCACGAAAGGCACAGTAGGAGCAGGAGCAGGAGTCCTTACAGACATCGAAGCTATGAGGAGAGGCCTCATCGCAGTCGTAGCAGTAAGTATGATGACAGAGACAGAGACAGGGACTATCATCACGACAGTGATAGCAGGAGACGCAGGACTTCAAGTCCTGGACGGAGAAGGGGAAGGAGTCCTAGTCGCAGTCGAAGTCCTGTTGGAAGGAGGAATCGTAGTCCAGTCAGGGATGGTAGCGAGGAGAGACGTGCGAGAATTGAGCAGTGGAACAGGGAAAGGGAAGAGAAGGAACCCACAAGTAAGGTTAATACAGAGGAAACCAGTAATGGCCATAACGAGCACTCACAAAATGCTAGCGCATATCATGAGAATCAGCAGCAACGGGAATCTCCTCGGGAGGGATATTGA
Protein sequence

>MS.gene94649.t1

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTILLSNMYQRPDMITPGVDVHGQPIDPRKIQDHFEEFYEDLFDELSKYGDIESLNVCDNLADHMVGNVYVQFREEEHAANAVKNLTGRFYAGRPIIVDFSPVTDFREATCRQYEENTCNRGGYCNFMHLKRISRELRRQLFGKRHERHSRSRSRSPYRHRSYEERPHRSRSSKYDDRDRDRDYHHDSDSRRRRTSSPGRRRGRSPSRSRSPVGRRNRSPVRDGSEERRARIEQWNREREEKEPTSKVNTEETSNGHNEHSQNASAYHENQQQRESPREGY