AlfalfaGEDB | MS.gene95101

M. sativa cultivar XinJiangDaYe / MS.gene95101

KEGG: K08065
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0046982 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95101.t1	XP_013458655.1	95.6	136	6	0	1	136	1	136	5.20E-65	256.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95101.t1	O23310	75.2	117	23	2	1	113	1	115	1.4e-42	173.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95101.t1	I3TAW6	95.6	136	6	0	1	136	1	136	3.8e-65	256.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene95101.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049983	MS.gene95101	0.81451	1.49E-51	-1.69E-46
MS.gene050414	MS.gene95101	0.817528	3.15E-52	-1.69E-46
MS.gene053931	MS.gene95101	0.835175	1.96E-56	-1.69E-46
MS.gene058008	MS.gene95101	0.824593	7.42E-54	-1.69E-46
MS.gene058009	MS.gene95101	0.819853	9.34E-53	-1.69E-46
MS.gene058781	MS.gene95101	0.812757	3.61E-51	-1.69E-46
MS.gene060244	MS.gene95101	0.813384	2.63E-51	-1.69E-46
MS.gene060935	MS.gene95101	0.814375	1.59E-51	-1.69E-46
MS.gene060937	MS.gene95101	0.811331	7.38E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95101.t1	MTR_4g133938	95.588	136	6	0	1	136	1	136	4.51e-94	267
MS.gene95101.t1	MTR_2g026710	74.615	130	28	2	1	130	1	125	4.27e-64	193
MS.gene95101.t1	MTR_5g095740	74.167	120	27	2	1	116	1	120	6.49e-59	180
MS.gene95101.t1	MTR_3g058980	71.074	121	27	1	1	113	1	121	2.16e-58	179
MS.gene95101.t1	MTR_1g072790	69.748	119	33	2	10	127	22	138	3.08e-54	168
MS.gene95101.t1	MTR_8g091720	80.412	97	19	0	20	116	29	125	4.20e-54	169
MS.gene95101.t1	MTR_8g093920	78.723	94	20	0	20	113	41	134	1.34e-53	168
MS.gene95101.t1	MTR_0392s0020	67.857	112	30	2	6	113	3	112	3.27e-52	162
MS.gene95101.t1	MTR_4g119500	67.273	110	31	1	4	113	12	116	3.27e-52	161
MS.gene95101.t1	MTR_1g088860	75.532	94	23	0	20	113	37	130	3.92e-52	163
MS.gene95101.t1	MTR_7g061270	64.286	126	42	1	7	129	14	139	1.09e-51	161
MS.gene95101.t1	MTR_7g100650	64.286	126	42	1	7	129	14	139	1.61e-51	161
MS.gene95101.t1	MTR_4g133952	65.957	94	32	0	20	113	59	152	7.01e-45	146
MS.gene95101.t1	MTR_1g083070	64.773	88	31	0	21	108	6	93	1.70e-41	134
MS.gene95101.t1	MTR_1g039040	56.436	101	42	1	13	113	2	100	3.15e-39	130
MS.gene95101.t1	MTR_1g029070	54.545	88	40	0	20	107	53	140	2.58e-28	102
MS.gene95101.t1	MTR_5g095900	46.667	105	51	2	7	108	3	105	5.13e-25	94.4
MS.gene95101.t1	MTR_1g029100	40.789	76	45	0	24	99	7	82	7.84e-19	75.9
MS.gene95101.t1	MTR_1g028480	50.000	66	33	0	49	114	19	84	2.49e-18	75.1
MS.gene95101.t1	MTR_2g056000	30.952	84	58	0	24	107	46	129	2.14e-13	64.3
MS.gene95101.t1	MTR_4g112380	30.952	84	58	0	24	107	16	99	3.82e-13	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95101.t1	AT4G14540	74.576	118	24	2	1	114	1	116	1.40e-59	181
MS.gene95101.t1	AT5G47640	81.053	95	18	0	20	114	28	122	1.97e-54	169
MS.gene95101.t1	AT2G38880	64.516	124	40	3	9	130	12	133	2.01e-51	160
MS.gene95101.t1	AT2G47810	75.532	94	23	0	20	113	52	145	2.12e-51	160
MS.gene95101.t1	AT2G38880	64.516	124	40	3	9	130	12	133	3.14e-51	159
MS.gene95101.t1	AT2G38880	64.516	124	40	3	9	130	12	133	3.14e-51	159
MS.gene95101.t1	AT2G38880	64.516	124	40	3	9	130	12	133	3.14e-51	159
MS.gene95101.t1	AT2G13570	75.532	94	23	0	20	113	37	130	1.03e-50	160
MS.gene95101.t1	AT2G38880	64.228	123	40	3	9	130	12	131	9.27e-50	155
MS.gene95101.t1	AT2G37060	68.807	109	30	2	7	112	16	123	1.53e-49	156
MS.gene95101.t1	AT2G37060	68.807	109	30	2	7	112	16	123	1.53e-49	156
MS.gene95101.t1	AT2G37060	68.807	109	30	2	7	112	16	123	1.53e-49	156
MS.gene95101.t1	AT2G38880	72.549	102	25	2	9	109	12	111	1.57e-49	154
MS.gene95101.t1	AT3G53340	73.118	93	25	0	20	112	30	122	1.57e-49	156
MS.gene95101.t1	AT3G53340	73.118	93	25	0	20	112	30	122	1.57e-49	156
MS.gene95101.t1	AT2G38880	72.549	102	25	2	9	109	12	111	2.58e-49	155
MS.gene95101.t1	AT2G38880	72.549	102	25	2	9	109	12	111	2.58e-49	155
MS.gene95101.t1	AT2G38880	72.549	102	25	2	9	109	12	111	2.58e-49	155
MS.gene95101.t1	AT2G38880	72.549	102	25	2	9	109	12	111	5.43e-49	154
MS.gene95101.t1	AT2G38880	72.549	102	25	2	9	109	12	111	5.43e-49	154
MS.gene95101.t1	AT3G53340	73.333	90	24	0	20	109	30	119	1.34e-48	152
MS.gene95101.t1	AT3G53340	72.527	91	25	0	20	110	30	120	1.30e-47	150
MS.gene95101.t1	AT3G53340	72.527	91	25	0	20	110	30	120	1.30e-47	150
MS.gene95101.t1	AT5G47670	62.887	97	36	0	20	116	30	126	1.99e-44	144
MS.gene95101.t1	AT5G47670	62.887	97	36	0	20	116	59	155	3.98e-44	144
MS.gene95101.t1	AT5G47670	62.887	97	36	0	20	116	59	155	3.98e-44	144
MS.gene95101.t1	AT1G09030	56.522	115	47	1	20	131	4	118	8.64e-44	140
MS.gene95101.t1	AT1G21970	57.143	98	42	0	20	117	60	157	1.12e-40	135
MS.gene95101.t1	AT5G08190	36.082	97	60	1	24	118	16	112	1.23e-18	77.4
MS.gene95101.t1	AT5G08190	35.714	98	60	2	24	118	16	113	7.57e-17	72.8
MS.gene95101.t1	AT5G23090	31.959	97	64	1	24	118	16	112	1.65e-15	69.3
MS.gene95101.t1	AT5G23090	34.118	85	54	1	24	106	16	100	5.61e-14	65.1
MS.gene95101.t1	AT5G23090	34.118	85	54	1	24	106	16	100	5.61e-14	65.1
MS.gene95101.t1	AT5G23090	34.118	85	54	1	24	106	16	100	5.61e-14	65.1
MS.gene95101.t1	AT5G23090	32.941	85	54	2	25	106	3	87	9.02e-11	56.6

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATCAGGTGGTCAAGCTTC+AGG	0.278269	8.2:-768419	MS.gene95101:CDS
GGGTTACCTTCACAAGTATA+GGG	0.382046	8.2:-768138	MS.gene95101:CDS
GATCATGAAGAAAGCTTTAC+CGG	0.392387	8.2:-768345	MS.gene95101:CDS
AGAATATGCAGAGCCTCTTA+AGG	0.393028	8.2:-768159	MS.gene95101:CDS
AGGGTTACCTTCACAAGTAT+AGG	0.429699	8.2:-768139	MS.gene95101:CDS
TTCGATATCTTTGCTCTCGC+CGG	0.458504	8.2:+768326	None:intergenic
GAGTTCATAAGCTTCATAAC+AGG	0.460674	8.2:-768269	MS.gene95101:CDS
GATCCTACCAACGTTTGCTA+TGG	0.475579	8.2:+768364	None:intergenic
GCAGAATCAGATGATGAATC+AGG	0.475967	8.2:-768434	MS.gene95101:CDS
GAGAAGAGGAAGACAATAAA+CGG	0.477283	8.2:-768221	MS.gene95101:CDS
TTGTGAAGGTAACCCTTAAG+AGG	0.483388	8.2:+768146	None:intergenic
GAATATGCAGAGCCTCTTAA+GGG	0.488554	8.2:-768158	MS.gene95101:CDS
AATCAGGTGGTCAAGCTTCA+GGG	0.501937	8.2:-768418	MS.gene95101:CDS
GAATATGATTGGTAGTAGTA+AGG	0.514281	8.2:-768087	MS.gene95101:CDS
GCAGAGAATTACTGCAAGAA+AGG	0.517934	8.2:-768394	MS.gene95101:CDS
CAATTTCCCTATACTTGTGA+AGG	0.523802	8.2:+768132	None:intergenic
GTTGCAAATATAATCAACCT+TGG	0.534009	8.2:+768036	None:intergenic
ATCCTACCAACGTTTGCTAT+GGG	0.537868	8.2:+768365	None:intergenic
AAAAGTGTCAGAAAGAGAAG+AGG	0.551950	8.2:-768235	MS.gene95101:CDS
AGTATTGATCGATTCTTCCA+AGG	0.558864	8.2:-768053	MS.gene95101:CDS
TTCAAAGCCAAGTGTAGTCA+TGG	0.563942	8.2:+768181	None:intergenic
GTGTGGGCCATGACTACACT+TGG	0.565546	8.2:-768188	MS.gene95101:CDS
CACAAGTATAGGGAAATTGA+CGG	0.568771	8.2:-768128	MS.gene95101:CDS
TACCAACGTTTGCTATGGGA+AGG	0.599059	8.2:+768369	None:intergenic
TTCCCATAGCAAACGTTGGT+AGG	0.621000	8.2:-768367	MS.gene95101:CDS
CTCCTTCCCATAGCAAACGT+TGG	0.626066	8.2:-768371	MS.gene95101:CDS
GGTAGTAGTAAGGAGCAGCA+AGG	0.645799	8.2:-768077	MS.gene95101:CDS
TAAACGGTGATGATCTTGTG+TGG	0.661494	8.2:-768205	MS.gene95101:CDS
AAACGGTGATGATCTTGTGT+GGG	0.691215	8.2:-768204	MS.gene95101:CDS
GAATCAGATGATGAATCAGG+TGG	0.704177	8.2:-768431	MS.gene95101:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTTCTATGAATATGAT+TGG	-	chr8.2:768388-768407	MS.gene95101:CDS	15.0%
!	GAATATGATTGGTAGTAGTA+AGG	-	chr8.2:768399-768418	MS.gene95101:CDS	30.0%
	CAATTTCCCTATACTTGTGA+AGG	+	chr8.2:768357-768376	None:intergenic	35.0%
	CACAAGTATAGGGAAATTGA+CGG	-	chr8.2:768358-768377	MS.gene95101:CDS	35.0%
	GAGAAGAGGAAGACAATAAA+CGG	-	chr8.2:768265-768284	MS.gene95101:CDS	35.0%
	GAGTTCATAAGCTTCATAAC+AGG	-	chr8.2:768217-768236	MS.gene95101:CDS	35.0%
	GATCATGAAGAAAGCTTTAC+CGG	-	chr8.2:768141-768160	MS.gene95101:CDS	35.0%
!!	AAAAGTGTCAGAAAGAGAAG+AGG	-	chr8.2:768251-768270	MS.gene95101:CDS	35.0%
!!	AGTATTGATCGATTCTTCCA+AGG	-	chr8.2:768433-768452	MS.gene95101:CDS	35.0%
	AAACGGTGATGATCTTGTGT+GGG	-	chr8.2:768282-768301	MS.gene95101:CDS	40.0%
	AGAATATGCAGAGCCTCTTA+AGG	-	chr8.2:768327-768346	MS.gene95101:CDS	40.0%
	AGGGTTACCTTCACAAGTAT+AGG	-	chr8.2:768347-768366	MS.gene95101:CDS	40.0%
	GAATATGCAGAGCCTCTTAA+GGG	-	chr8.2:768328-768347	MS.gene95101:CDS	40.0%
	GAATCAGATGATGAATCAGG+TGG	-	chr8.2:768055-768074	MS.gene95101:CDS	40.0%
	GCAGAATCAGATGATGAATC+AGG	-	chr8.2:768052-768071	MS.gene95101:CDS	40.0%
	GCAGAGAATTACTGCAAGAA+AGG	-	chr8.2:768092-768111	MS.gene95101:CDS	40.0%
	GGGTTACCTTCACAAGTATA+GGG	-	chr8.2:768348-768367	MS.gene95101:CDS	40.0%
	TAAACGGTGATGATCTTGTG+TGG	-	chr8.2:768281-768300	MS.gene95101:CDS	40.0%
	TTCAAAGCCAAGTGTAGTCA+TGG	+	chr8.2:768308-768327	None:intergenic	40.0%
	TTGTGAAGGTAACCCTTAAG+AGG	+	chr8.2:768343-768362	None:intergenic	40.0%
!!	ATCCTACCAACGTTTGCTAT+GGG	+	chr8.2:768124-768143	None:intergenic	40.0%
	AATCAGGTGGTCAAGCTTCA+GGG	-	chr8.2:768068-768087	MS.gene95101:CDS	45.0%
	TTCCCATAGCAAACGTTGGT+AGG	-	chr8.2:768119-768138	MS.gene95101:CDS	45.0%
	TTCGATATCTTTGCTCTCGC+CGG	+	chr8.2:768163-768182	None:intergenic	45.0%
!	GATCCTACCAACGTTTGCTA+TGG	+	chr8.2:768125-768144	None:intergenic	45.0%
!!	TACCAACGTTTGCTATGGGA+AGG	+	chr8.2:768120-768139	None:intergenic	45.0%
	CTCCTTCCCATAGCAAACGT+TGG	-	chr8.2:768115-768134	MS.gene95101:CDS	50.0%
	GAATCAGGTGGTCAAGCTTC+AGG	-	chr8.2:768067-768086	MS.gene95101:CDS	50.0%
	GGTAGTAGTAAGGAGCAGCA+AGG	-	chr8.2:768409-768428	MS.gene95101:CDS	50.0%
	GTGTGGGCCATGACTACACT+TGG	-	chr8.2:768298-768317	MS.gene95101:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	768049	768459	768049	ID=MS.gene95101
chr8.2	mRNA	768049	768459	768049	ID=MS.gene95101.t1;Parent=MS.gene95101
chr8.2	exon	768049	768459	768049	ID=MS.gene95101.t1.exon1;Parent=MS.gene95101.t1
chr8.2	CDS	768049	768459	768049	ID=cds.MS.gene95101.t1;Parent=MS.gene95101.t1

Gene Sequence

>MS.gene95101

ATGGCAGAATCAGATGATGAATCAGGTGGTCAAGCTTCAGGGAGCAGAGAATTACTGCAAGAAAGGCTCCTTCCCATAGCAAACGTTGGTAGGATCATGAAGAAAGCTTTACCGGCGAGAGCAAAGATATCGAAAGAAGCTAAAGAAACGATGCAAGAGTGTGTATCAGAGTTCATAAGCTTCATAACAGGTGAAGCATCTGAAAAGTGTCAGAAAGAGAAGAGGAAGACAATAAACGGTGATGATCTTGTGTGGGCCATGACTACACTTGGCTTTGAAGAATATGCAGAGCCTCTTAAGGGTTACCTTCACAAGTATAGGGAAATTGACGGTGACAAGAATTTTTCTATGAATATGATTGGTAGTAGTAAGGAGCAGCAAGGTAGTATTGATCGATTCTTCCAAGGTTGA

Protein sequence

>MS.gene95101.t1

MAESDDESGGQASGSRELLQERLLPIANVGRIMKKALPARAKISKEAKETMQECVSEFISFITGEASEKCQKEKRKTINGDDLVWAMTTLGFEEYAEPLKGYLHKYREIDGDKNFSMNMIGSSKEQQGSIDRFFQG