Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95155.t1 | XP_027188200.1 | 47.8 | 203 | 103 | 1 | 1 | 203 | 117 | 316 | 3.40E-36 | 161.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95155.t1 | Q8L727 | 38.0 | 129 | 70 | 2 | 1 | 123 | 337 | 461 | 4.1e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95155.t1 | A0A3Q7YDC8 | 47.8 | 203 | 103 | 1 | 1 | 203 | 117 | 316 | 2.5e-36 | 161.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene95155 | MS.gene90825 | PPI |
| MS.gene95155 | MS.gene97346 | PPI |
| MS.gene95155 | MS.gene029093 | PPI |
| MS.gene066439 | MS.gene95155 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95155.t1 | MTR_7g088160 | 42.544 | 228 | 103 | 2 | 1 | 203 | 165 | 389 | 1.49e-52 | 173 |
| MS.gene95155.t1 | MTR_4g063670 | 44.860 | 214 | 98 | 5 | 1 | 202 | 114 | 319 | 3.08e-38 | 134 |
| MS.gene95155.t1 | MTR_7g107340 | 39.594 | 197 | 109 | 3 | 5 | 200 | 145 | 332 | 4.18e-31 | 116 |
| MS.gene95155.t1 | MTR_0867s0010 | 43.925 | 214 | 100 | 5 | 1 | 202 | 114 | 319 | 4.03e-30 | 113 |
| MS.gene95155.t1 | MTR_4g083510 | 40.278 | 216 | 106 | 7 | 1 | 202 | 114 | 320 | 1.12e-26 | 104 |
| MS.gene95155.t1 | MTR_1g051185 | 51.402 | 107 | 44 | 2 | 1 | 107 | 89 | 187 | 8.45e-26 | 100 |
| MS.gene95155.t1 | MTR_7g114170 | 34.184 | 196 | 80 | 5 | 5 | 200 | 141 | 287 | 1.07e-14 | 71.2 |
| MS.gene95155.t1 | MTR_8g015750 | 33.654 | 208 | 102 | 6 | 7 | 202 | 3 | 186 | 6.41e-14 | 67.4 |
| MS.gene95155.t1 | MTR_4g046740 | 35.659 | 129 | 64 | 5 | 78 | 202 | 23 | 136 | 4.43e-13 | 63.9 |
| MS.gene95155.t1 | MTR_3g069460 | 50.000 | 60 | 30 | 0 | 1 | 60 | 114 | 173 | 1.28e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95155.t1 | AT5G41240 | 37.984 | 129 | 70 | 2 | 1 | 123 | 337 | 461 | 3.80e-19 | 85.1 |
| MS.gene95155.t1 | AT5G41220 | 37.008 | 127 | 69 | 3 | 1 | 120 | 335 | 457 | 9.82e-16 | 75.5 |
| MS.gene95155.t1 | AT5G41220 | 37.008 | 127 | 69 | 3 | 1 | 120 | 277 | 399 | 1.02e-15 | 75.1 |
| MS.gene95155.t1 | AT5G41220 | 37.008 | 127 | 69 | 3 | 1 | 120 | 285 | 407 | 1.08e-15 | 75.1 |
Find 33 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGGATTCTTCAGTAAAT+TGG | 0.187266 | 8.2:+1380714 | None:intergenic |
| GTAAATTGGCATGACCATTT+AGG | 0.268868 | 8.2:+1380728 | None:intergenic |
| TACTAAAAGATGAACCTAAA+TGG | 0.349471 | 8.2:-1380742 | MS.gene95155:CDS |
| TTCACTTGAGTTGTATTGTC+CGG | 0.371973 | 8.2:+1380619 | None:intergenic |
| AGAACAAAGAATTCTGCTTC+AGG | 0.392497 | 8.2:-1380689 | MS.gene95155:CDS |
| AGAATTCTTTGTTCTCTTGT+TGG | 0.394331 | 8.2:+1380697 | None:intergenic |
| GTTAGATCCACATGAACAAT+AGG | 0.411169 | 8.2:+1380527 | None:intergenic |
| TCACTTGAGTTGTATTGTCC+GGG | 0.435029 | 8.2:+1380620 | None:intergenic |
| TCACCACTTGTAGGCGTGTC+CGG | 0.441723 | 8.2:+1380644 | None:intergenic |
| AGTAGAGTGGTTCGTCCGAT+TGG | 0.444657 | 8.2:-1380596 | MS.gene95155:CDS |
| ATCTCTTGATGGATACATCT+AGG | 0.458045 | 8.2:-1380379 | MS.gene95155:CDS |
| TGAGCTTCACTATCTCTTGA+TGG | 0.465437 | 8.2:-1380390 | MS.gene95155:CDS |
| ATGGTCGAAGGTGAACTTGA+TGG | 0.477920 | 8.2:-1380906 | None:intergenic |
| CTACACATCCTCTTCTAATC+CGG | 0.479246 | 8.2:-1380663 | MS.gene95155:CDS |
| TGTCCGGGGTCACCACTTGT+AGG | 0.492325 | 8.2:+1380635 | None:intergenic |
| GTAGAGTGGTTCGTCCGATT+GGG | 0.497308 | 8.2:-1380595 | MS.gene95155:CDS |
| TTGTTCATGTGGATCTAACT+AGG | 0.507003 | 8.2:-1380523 | MS.gene95155:CDS |
| GACAAAAGCAGCTAAAAGAA+AGG | 0.508350 | 8.2:-1380573 | MS.gene95155:CDS |
| GCATGAAGGTAACAAGTTGA+AGG | 0.526954 | 8.2:-1380444 | MS.gene95155:CDS |
| CGACATTATGCAAGATGCAT+TGG | 0.532468 | 8.2:-1380819 | MS.gene95155:CDS |
| ACAAAAGCAGCTAAAAGAAA+GGG | 0.543467 | 8.2:-1380572 | MS.gene95155:CDS |
| GCCTATTCAAGAAAGAAAAG+TGG | 0.554764 | 8.2:-1380854 | MS.gene95155:CDS |
| TCATGTGGATCTAACTAGGA+TGG | 0.578161 | 8.2:-1380519 | MS.gene95155:CDS |
| TGAAACACCTATTGTTCATG+TGG | 0.578577 | 8.2:-1380534 | MS.gene95155:CDS |
| CGGATTAGAAGAGGATGTGT+AGG | 0.590924 | 8.2:+1380664 | None:intergenic |
| ACGCCTACAAGTGGTGACCC+CGG | 0.604163 | 8.2:-1380638 | MS.gene95155:CDS |
| CACTTGAGTTGTATTGTCCG+GGG | 0.607801 | 8.2:+1380621 | None:intergenic |
| ATTGCACGTGGTGTGCATGA+AGG | 0.607967 | 8.2:-1380458 | MS.gene95155:CDS |
| AATCCGGACACGCCTACAAG+TGG | 0.635438 | 8.2:-1380647 | MS.gene95155:CDS |
| AGGCGTGTCCGGATTAGAAG+AGG | 0.638071 | 8.2:+1380655 | None:intergenic |
| CAACTCAAGTGAAAGTAGAG+TGG | 0.640911 | 8.2:-1380609 | MS.gene95155:CDS |
| GCTTTAGAAGATATTGCACG+TGG | 0.699659 | 8.2:-1380470 | MS.gene95155:CDS |
| GGATACATCTAGGATGACAG+AGG | 0.702928 | 8.2:-1380369 | MS.gene95155:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GGAAAAAGAATTGCAAATTA+AGG | - | chr8.2:1380780-1380799 | MS.gene95155:CDS | 25.0% |
| ! | TACTAAAAGATGAACCTAAA+TGG | - | chr8.2:1380461-1380480 | MS.gene95155:CDS | 25.0% |
| ACAAAAGCAGCTAAAAGAAA+GGG | - | chr8.2:1380631-1380650 | MS.gene95155:CDS | 30.0% | |
| AGAATTCTTTGTTCTCTTGT+TGG | + | chr8.2:1380509-1380528 | None:intergenic | 30.0% | |
| TGTTGGATTCTTCAGTAAAT+TGG | + | chr8.2:1380492-1380511 | None:intergenic | 30.0% | |
| ! | TCTTTTAGTAATTTCCATGC+TGG | + | chr8.2:1380451-1380470 | None:intergenic | 30.0% |
| !! | ACCACTTTTCTTTCTTGAAT+AGG | + | chr8.2:1380353-1380372 | None:intergenic | 30.0% |
| AGAACAAAGAATTCTGCTTC+AGG | - | chr8.2:1380514-1380533 | MS.gene95155:CDS | 35.0% | |
| GACAAAAGCAGCTAAAAGAA+AGG | - | chr8.2:1380630-1380649 | MS.gene95155:CDS | 35.0% | |
| GCCTATTCAAGAAAGAAAAG+TGG | - | chr8.2:1380349-1380368 | MS.gene95155:CDS | 35.0% | |
| GTAAATTGGCATGACCATTT+AGG | + | chr8.2:1380478-1380497 | None:intergenic | 35.0% | |
| GTTAGATCCACATGAACAAT+AGG | + | chr8.2:1380679-1380698 | None:intergenic | 35.0% | |
| TGAAACACCTATTGTTCATG+TGG | - | chr8.2:1380669-1380688 | MS.gene95155:CDS | 35.0% | |
| TTGTTCATGTGGATCTAACT+AGG | - | chr8.2:1380680-1380699 | MS.gene95155:CDS | 35.0% | |
| ! | ATCTCTTGATGGATACATCT+AGG | - | chr8.2:1380824-1380843 | MS.gene95155:CDS | 35.0% |
| ! | TTCACTTGAGTTGTATTGTC+CGG | + | chr8.2:1380587-1380606 | None:intergenic | 35.0% |
| CAACTCAAGTGAAAGTAGAG+TGG | - | chr8.2:1380594-1380613 | MS.gene95155:CDS | 40.0% | |
| CGACATTATGCAAGATGCAT+TGG | - | chr8.2:1380384-1380403 | MS.gene95155:CDS | 40.0% | |
| CTACACATCCTCTTCTAATC+CGG | - | chr8.2:1380540-1380559 | MS.gene95155:CDS | 40.0% | |
| TCATGTGGATCTAACTAGGA+TGG | - | chr8.2:1380684-1380703 | MS.gene95155:CDS | 40.0% | |
| TGAGCTTCACTATCTCTTGA+TGG | - | chr8.2:1380813-1380832 | MS.gene95155:CDS | 40.0% | |
| ! | GCATGAAGGTAACAAGTTGA+AGG | - | chr8.2:1380759-1380778 | MS.gene95155:CDS | 40.0% |
| ! | GTTTTATGTTAGTGCCAGCA+TGG | - | chr8.2:1380434-1380453 | MS.gene95155:CDS | 40.0% |
| ! | TCACTTGAGTTGTATTGTCC+GGG | + | chr8.2:1380586-1380605 | None:intergenic | 40.0% |
| !! | GCTTTAGAAGATATTGCACG+TGG | - | chr8.2:1380733-1380752 | MS.gene95155:CDS | 40.0% |
| !!! | TTAGCTGCTTTTGTCCCAAT+CGG | + | chr8.2:1380625-1380644 | None:intergenic | 40.0% |
| ! | CACTTGAGTTGTATTGTCCG+GGG | + | chr8.2:1380585-1380604 | None:intergenic | 45.0% |
| ! | CGGATTAGAAGAGGATGTGT+AGG | + | chr8.2:1380542-1380561 | None:intergenic | 45.0% |
| ! | GGATACATCTAGGATGACAG+AGG | - | chr8.2:1380834-1380853 | MS.gene95155:CDS | 45.0% |
| AGTAGAGTGGTTCGTCCGAT+TGG | - | chr8.2:1380607-1380626 | MS.gene95155:CDS | 50.0% | |
| ATTGCACGTGGTGTGCATGA+AGG | - | chr8.2:1380745-1380764 | MS.gene95155:CDS | 50.0% | |
| GTAGAGTGGTTCGTCCGATT+GGG | - | chr8.2:1380608-1380627 | MS.gene95155:CDS | 50.0% | |
| AATCCGGACACGCCTACAAG+TGG | - | chr8.2:1380556-1380575 | MS.gene95155:CDS | 55.0% | |
| TCACCACTTGTAGGCGTGTC+CGG | + | chr8.2:1380562-1380581 | None:intergenic | 55.0% | |
| ! | AGGCGTGTCCGGATTAGAAG+AGG | + | chr8.2:1380551-1380570 | None:intergenic | 55.0% |
| ACGCCTACAAGTGGTGACCC+CGG | - | chr8.2:1380565-1380584 | MS.gene95155:CDS | 60.0% | |
| TGTCCGGGGTCACCACTTGT+AGG | + | chr8.2:1380571-1380590 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 1380307 | 1380918 | 1380307 | ID=MS.gene95155 |
| chr8.2 | mRNA | 1380307 | 1380918 | 1380307 | ID=MS.gene95155.t1;Parent=MS.gene95155 |
| chr8.2 | exon | 1380307 | 1380918 | 1380307 | ID=MS.gene95155.t1.exon1;Parent=MS.gene95155.t1 |
| chr8.2 | CDS | 1380307 | 1380918 | 1380307 | ID=cds.MS.gene95155.t1;Parent=MS.gene95155.t1 |
| Gene Sequence |
| Protein sequence |
