AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95156


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95156.t1 MTR_7g088160 43.769 329 146 3 18 321 75 389 1.58e-85 263
MS.gene95156.t1 MTR_4g063670 43.438 320 150 9 15 320 17 319 6.39e-62 200
MS.gene95156.t1 MTR_0867s0010 43.750 320 149 8 15 320 17 319 4.49e-59 192
MS.gene95156.t1 MTR_4g083510 40.683 322 157 10 15 320 17 320 3.31e-50 169
MS.gene95156.t1 MTR_7g107340 36.184 304 146 4 16 318 76 332 2.56e-49 167
MS.gene95156.t1 MTR_1g051185 47.020 151 70 4 77 225 45 187 4.82e-35 128
MS.gene95156.t1 MTR_3g069460 41.566 166 86 3 15 178 17 173 1.59e-34 124
MS.gene95156.t1 MTR_7g114170 33.663 303 125 8 16 318 61 287 1.91e-30 117
MS.gene95156.t1 MTR_6g081150 36.413 184 71 5 77 260 40 177 5.01e-25 99.8
MS.gene95156.t1 MTR_5g066280 24.503 302 200 5 20 319 55 330 4.34e-22 95.1
MS.gene95156.t1 MTR_4g026120 24.503 302 200 5 20 319 55 330 1.10e-21 94.0
MS.gene95156.t1 MTR_4g036435 24.503 302 200 5 20 319 55 330 1.10e-21 94.0
MS.gene95156.t1 MTR_3g067785 24.503 302 200 5 20 319 55 330 1.26e-21 94.0
MS.gene95156.t1 MTR_4g013220 24.503 302 200 5 20 319 55 330 1.36e-21 93.6
MS.gene95156.t1 MTR_1g106885 24.503 302 200 5 20 319 116 391 1.18e-20 92.0
MS.gene95156.t1 MTR_2g022120 24.503 302 200 5 20 319 119 394 1.19e-20 92.0
MS.gene95156.t1 MTR_5g077245 24.172 302 201 5 20 319 108 383 6.21e-20 89.7
MS.gene95156.t1 MTR_2g072860 28.659 164 112 2 18 181 29 187 5.84e-19 85.9
MS.gene95156.t1 MTR_7g461350 28.333 180 121 3 1 180 83 254 1.32e-18 85.9
MS.gene95156.t1 MTR_6g016670 28.333 180 121 3 1 180 83 254 1.32e-18 85.9
MS.gene95156.t1 MTR_3g464310 28.333 180 121 3 1 180 83 254 1.32e-18 85.9
MS.gene95156.t1 MTR_6g092533 27.329 161 114 1 20 180 119 276 1.48e-18 85.9
MS.gene95156.t1 MTR_3g464170 27.778 180 122 3 1 180 83 254 2.18e-18 85.1
MS.gene95156.t1 MTR_1g100375 27.778 180 122 3 1 180 83 254 2.18e-18 85.1
MS.gene95156.t1 MTR_6g051405 28.834 163 111 2 18 180 97 254 2.40e-18 85.1
MS.gene95156.t1 MTR_3g450670 28.221 163 112 2 18 180 93 250 5.96e-18 84.0
MS.gene95156.t1 MTR_1g471070 28.221 163 112 2 18 180 93 250 5.96e-18 84.0
MS.gene95156.t1 MTR_7g062380 23.626 182 121 1 48 229 15 178 5.37e-15 73.9
MS.gene95156.t1 MTR_6g453050 24.540 163 116 3 20 180 55 212 1.66e-13 70.5
MS.gene95156.t1 MTR_8g015750 33.654 208 102 6 125 320 3 186 3.81e-13 67.4
MS.gene95156.t1 MTR_4g046090 26.316 152 94 1 79 230 71 204 6.41e-13 68.2
MS.gene95156.t1 MTR_7g083520 23.125 160 120 1 22 181 31 187 1.23e-12 67.8
MS.gene95156.t1 MTR_4g046740 35.659 129 64 5 196 320 23 136 1.69e-12 64.3
MS.gene95156.t1 MTR_1g086750 27.966 118 85 0 59 176 26 143 2.08e-12 65.9
MS.gene95156.t1 MTR_8g015740 42.857 77 32 1 25 89 54 130 2.97e-11 60.5
MS.gene95156.t1 MTR_2g023560 63.636 44 16 0 58 101 62 105 3.16e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95156.t1 AT5G41220 38.889 198 108 4 50 238 214 407 1.35e-33 129
MS.gene95156.t1 AT5G41220 38.889 198 108 4 50 238 206 399 1.36e-33 129
MS.gene95156.t1 AT5G41220 38.889 198 108 4 50 238 264 457 2.27e-33 129
MS.gene95156.t1 AT5G41240 37.624 202 112 4 50 241 264 461 2.86e-32 126
MS.gene95156.t1 AT3G47680 32.558 215 117 8 22 227 14 209 1.46e-23 98.6
MS.gene95156.t1 AT1G66235 25.510 196 119 5 59 236 7 193 1.10e-12 67.8

Find 53 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGATTCTTCAGTAAAT+TGG 0.187266 8.4:+1438425 None:intergenic
GTAAATTGGCATGACCATTT+AGG 0.268868 8.4:+1438439 None:intergenic
TAGAAATATTTAACCATGAT+TGG 0.280172 8.4:+1438758 None:intergenic
ATCATGGTTAAATATTTCTA+AGG 0.308295 8.4:-1438755 MS.gene95156:CDS
TACTAAAAGATGAACCTAAA+TGG 0.349471 8.4:-1438453 MS.gene95156:CDS
TTCACTTGAGTTGTATTGTC+CGG 0.371973 8.4:+1438330 None:intergenic
AGAACAAAGAATTCTGCTTC+AGG 0.392497 8.4:-1438400 MS.gene95156:CDS
AGAATTCTTTGTTCTCTTGT+TGG 0.394331 8.4:+1438408 None:intergenic
GTTAGATCCACATGAACAAT+AGG 0.411169 8.4:+1438238 None:intergenic
TCACTTGAGTTGTATTGTCC+GGG 0.435029 8.4:+1438331 None:intergenic
TCACCACTTGTAGGCGTGTC+CGG 0.441723 8.4:+1438355 None:intergenic
AGTAGAGTGGTTCGTCCGAT+TGG 0.444657 8.4:-1438307 MS.gene95156:CDS
ATCTCTTGATGGATACATCT+AGG 0.458045 8.4:-1438090 MS.gene95156:CDS
ATACACTTCTTATCCAATCA+TGG 0.460631 8.4:-1438771 MS.gene95156:CDS
AGGAGAAAATATATAGAGAA+AGG 0.462201 8.4:-1438810 MS.gene95156:CDS
TGAGCTTCACTATCTCTTGA+TGG 0.465437 8.4:-1438101 MS.gene95156:CDS
TTTGAAGAAATCTGAGCTGT+TGG 0.469253 8.4:+1438847 None:intergenic
ATGGTCGAAGGTGAACTTGA+TGG 0.477920 8.4:-1438617 MS.gene95156:CDS
CTACACATCCTCTTCTAATC+CGG 0.479246 8.4:-1438374 MS.gene95156:CDS
TGTCCGGGGTCACCACTTGT+AGG 0.492325 8.4:+1438346 None:intergenic
ATTTCTAAGGATCCAATTGT+GGG 0.492615 8.4:-1438742 MS.gene95156:CDS
GTAGAGTGGTTCGTCCGATT+GGG 0.497308 8.4:-1438306 MS.gene95156:CDS
CAATATCGTGGTCATTTGAA+AGG 0.504699 8.4:-1438667 MS.gene95156:CDS
TTGTTCATGTGGATCTAACT+AGG 0.507003 8.4:-1438234 MS.gene95156:CDS
GACAAAAGCAGCTAAAAGAA+AGG 0.508350 8.4:-1438284 MS.gene95156:CDS
TATTTCTAAGGATCCAATTG+TGG 0.512026 8.4:-1438743 MS.gene95156:CDS
CCAACTTGAGAGGAAAATGA+TGG 0.520919 8.4:+1438904 None:intergenic
GCATGAAGGTAACAAGTTGA+AGG 0.526954 8.4:-1438155 MS.gene95156:CDS
CGACATTATGCAAGATGCAT+TGG 0.532468 8.4:-1438530 MS.gene95156:CDS
AATATCGTGGTCATTTGAAA+GGG 0.541210 8.4:-1438666 MS.gene95156:CDS
ACAAAAGCAGCTAAAAGAAA+GGG 0.543467 8.4:-1438283 MS.gene95156:CDS
GCCTATTCAAGAAAGAAAAG+TGG 0.554764 8.4:-1438565 MS.gene95156:CDS
AGTTGGGTTAGATAATATTG+AGG 0.557841 8.4:-1438887 MS.gene95156:CDS
TGAATCAAATTAAATGTCGA+TGG 0.569722 8.4:-1438636 MS.gene95156:CDS
TCATGTGGATCTAACTAGGA+TGG 0.578161 8.4:-1438230 MS.gene95156:CDS
TGAAACACCTATTGTTCATG+TGG 0.578577 8.4:-1438245 MS.gene95156:CDS
AATTAAATGTCGATGGTCGA+AGG 0.587022 8.4:-1438629 MS.gene95156:CDS
CGGATTAGAAGAGGATGTGT+AGG 0.590924 8.4:+1438375 None:intergenic
ATTATCTAACCCAACTTGAG+AGG 0.596041 8.4:+1438894 None:intergenic
TTCTAAGGATCCAATTGTGG+GGG 0.599880 8.4:-1438740 MS.gene95156:CDS
ACGCCTACAAGTGGTGACCC+CGG 0.604163 8.4:-1438349 MS.gene95156:CDS
CACTTGAGTTGTATTGTCCG+GGG 0.607801 8.4:+1438332 None:intergenic
ATTGCACGTGGTGTGCATGA+AGG 0.607967 8.4:-1438169 MS.gene95156:CDS
GATAATTATAATCAATATCG+TGG 0.614936 8.4:-1438679 MS.gene95156:CDS
AATCCGGACACGCCTACAAG+TGG 0.635438 8.4:-1438358 MS.gene95156:CDS
AGGCGTGTCCGGATTAGAAG+AGG 0.638071 8.4:+1438366 None:intergenic
TTTCTAAGGATCCAATTGTG+GGG 0.639337 8.4:-1438741 MS.gene95156:CDS
CAACTCAAGTGAAAGTAGAG+TGG 0.640911 8.4:-1438320 MS.gene95156:CDS
ATATAGAGAAAGGTTTACCA+CGG 0.696539 8.4:-1438800 MS.gene95156:CDS
GCTTTAGAAGATATTGCACG+TGG 0.699659 8.4:-1438181 MS.gene95156:CDS
GGATACATCTAGGATGACAG+AGG 0.702928 8.4:-1438080 MS.gene95156:CDS
AAGAAGTGTATCTTCCTCCG+TGG 0.715732 8.4:+1438783 None:intergenic
TAGAGAAAGGTTTACCACGG+AGG 0.724762 8.4:-1438797 MS.gene95156:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATTATAATCAATATCG+TGG - chr8.4:1438300-1438319 MS.gene95156:CDS 20.0%
!! TAGAAATATTTAACCATGAT+TGG + chr8.4:1438224-1438243 None:intergenic 20.0%
!! TTCAAAAGAAACAAAAACAA+AGG - chr8.4:1438149-1438168 MS.gene95156:CDS 20.0%
!!! ATCATGGTTAAATATTTCTA+AGG - chr8.4:1438224-1438243 MS.gene95156:CDS 20.0%
! AGGAGAAAATATATAGAGAA+AGG - chr8.4:1438169-1438188 MS.gene95156:CDS 25.0%
! GGAAAAAGAATTGCAAATTA+AGG - chr8.4:1438845-1438864 MS.gene95156:CDS 25.0%
! TACTAAAAGATGAACCTAAA+TGG - chr8.4:1438526-1438545 MS.gene95156:CDS 25.0%
! TGAATCAAATTAAATGTCGA+TGG - chr8.4:1438343-1438362 MS.gene95156:CDS 25.0%
AATATCGTGGTCATTTGAAA+GGG - chr8.4:1438313-1438332 MS.gene95156:CDS 30.0%
ACAAAAGCAGCTAAAAGAAA+GGG - chr8.4:1438696-1438715 MS.gene95156:CDS 30.0%
AGAATTCTTTGTTCTCTTGT+TGG + chr8.4:1438574-1438593 None:intergenic 30.0%
ATACACTTCTTATCCAATCA+TGG - chr8.4:1438208-1438227 MS.gene95156:CDS 30.0%
ATATAGAGAAAGGTTTACCA+CGG - chr8.4:1438179-1438198 MS.gene95156:CDS 30.0%
TGTTGGATTCTTCAGTAAAT+TGG + chr8.4:1438557-1438576 None:intergenic 30.0%
! AGTTGGGTTAGATAATATTG+AGG - chr8.4:1438092-1438111 MS.gene95156:CDS 30.0%
! ATTTCTAAGGATCCAATTGT+GGG - chr8.4:1438237-1438256 MS.gene95156:CDS 30.0%
! TATTTCTAAGGATCCAATTG+TGG - chr8.4:1438236-1438255 MS.gene95156:CDS 30.0%
! TCTTTTAGTAATTTCCATGC+TGG + chr8.4:1438516-1438535 None:intergenic 30.0%
!! ACCACTTTTCTTTCTTGAAT+AGG + chr8.4:1438418-1438437 None:intergenic 30.0%
AATTAAATGTCGATGGTCGA+AGG - chr8.4:1438350-1438369 MS.gene95156:CDS 35.0%
AGAACAAAGAATTCTGCTTC+AGG - chr8.4:1438579-1438598 MS.gene95156:CDS 35.0%
ATTATCTAACCCAACTTGAG+AGG + chr8.4:1438088-1438107 None:intergenic 35.0%
CAATATCGTGGTCATTTGAA+AGG - chr8.4:1438312-1438331 MS.gene95156:CDS 35.0%
GACAAAAGCAGCTAAAAGAA+AGG - chr8.4:1438695-1438714 MS.gene95156:CDS 35.0%
GCCTATTCAAGAAAGAAAAG+TGG - chr8.4:1438414-1438433 MS.gene95156:CDS 35.0%
GTAAATTGGCATGACCATTT+AGG + chr8.4:1438543-1438562 None:intergenic 35.0%
GTTAGATCCACATGAACAAT+AGG + chr8.4:1438744-1438763 None:intergenic 35.0%
TGAAACACCTATTGTTCATG+TGG - chr8.4:1438734-1438753 MS.gene95156:CDS 35.0%
TTGTTCATGTGGATCTAACT+AGG - chr8.4:1438745-1438764 MS.gene95156:CDS 35.0%
TTTGAAGAAATCTGAGCTGT+TGG + chr8.4:1438135-1438154 None:intergenic 35.0%
! ACCAAAGAGCAAGTTCTTTT+TGG - chr8.4:1438265-1438284 MS.gene95156:CDS 35.0%
! ATCTCTTGATGGATACATCT+AGG - chr8.4:1438889-1438908 MS.gene95156:CDS 35.0%
! CATCATTTTCCTCTCAAGTT+GGG - chr8.4:1438076-1438095 MS.gene95156:CDS 35.0%
! TCCAAAAAGAACTTGCTCTT+TGG + chr8.4:1438269-1438288 None:intergenic 35.0%
! TTCACTTGAGTTGTATTGTC+CGG + chr8.4:1438652-1438671 None:intergenic 35.0%
! TTTCTAAGGATCCAATTGTG+GGG - chr8.4:1438238-1438257 MS.gene95156:CDS 35.0%
CAACTCAAGTGAAAGTAGAG+TGG - chr8.4:1438659-1438678 MS.gene95156:CDS 40.0%
CCAACTTGAGAGGAAAATGA+TGG + chr8.4:1438078-1438097 None:intergenic 40.0%
CGACATTATGCAAGATGCAT+TGG - chr8.4:1438449-1438468 MS.gene95156:CDS 40.0%
CTACACATCCTCTTCTAATC+CGG - chr8.4:1438605-1438624 MS.gene95156:CDS 40.0%
TCATGTGGATCTAACTAGGA+TGG - chr8.4:1438749-1438768 MS.gene95156:CDS 40.0%
TGAGCTTCACTATCTCTTGA+TGG - chr8.4:1438878-1438897 MS.gene95156:CDS 40.0%
! CCATCATTTTCCTCTCAAGT+TGG - chr8.4:1438075-1438094 MS.gene95156:CDS 40.0%
! GCATGAAGGTAACAAGTTGA+AGG - chr8.4:1438824-1438843 MS.gene95156:CDS 40.0%
! GTTTTATGTTAGTGCCAGCA+TGG - chr8.4:1438499-1438518 MS.gene95156:CDS 40.0%
! TCACTTGAGTTGTATTGTCC+GGG + chr8.4:1438651-1438670 None:intergenic 40.0%
! TTCTAAGGATCCAATTGTGG+GGG - chr8.4:1438239-1438258 MS.gene95156:CDS 40.0%
!! GCTTTAGAAGATATTGCACG+TGG - chr8.4:1438798-1438817 MS.gene95156:CDS 40.0%
!!! TTAGCTGCTTTTGTCCCAAT+CGG + chr8.4:1438690-1438709 None:intergenic 40.0%
ATGGTCGAAGGTGAACTTGA+TGG - chr8.4:1438362-1438381 MS.gene95156:CDS 45.0%
TAGAGAAAGGTTTACCACGG+AGG - chr8.4:1438182-1438201 MS.gene95156:CDS 45.0%
! AAGAAGTGTATCTTCCTCCG+TGG + chr8.4:1438199-1438218 None:intergenic 45.0%
! CACTTGAGTTGTATTGTCCG+GGG + chr8.4:1438650-1438669 None:intergenic 45.0%
! CGGATTAGAAGAGGATGTGT+AGG + chr8.4:1438607-1438626 None:intergenic 45.0%
! GGATACATCTAGGATGACAG+AGG - chr8.4:1438899-1438918 MS.gene95156:CDS 45.0%
AGTAGAGTGGTTCGTCCGAT+TGG - chr8.4:1438672-1438691 MS.gene95156:CDS 50.0%
ATTGCACGTGGTGTGCATGA+AGG - chr8.4:1438810-1438829 MS.gene95156:CDS 50.0%
GTAGAGTGGTTCGTCCGATT+GGG - chr8.4:1438673-1438692 MS.gene95156:CDS 50.0%
!! CTTTGGTTTGCCCCCACAAT+TGG + chr8.4:1438252-1438271 None:intergenic 50.0%
AATCCGGACACGCCTACAAG+TGG - chr8.4:1438621-1438640 MS.gene95156:CDS 55.0%
TCACCACTTGTAGGCGTGTC+CGG + chr8.4:1438627-1438646 None:intergenic 55.0%
! AGGCGTGTCCGGATTAGAAG+AGG + chr8.4:1438616-1438635 None:intergenic 55.0%
ACGCCTACAAGTGGTGACCC+CGG - chr8.4:1438630-1438649 MS.gene95156:CDS 60.0%
TGTCCGGGGTCACCACTTGT+AGG + chr8.4:1438636-1438655 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1438018 1438983 1438018 ID=MS.gene95156
chr8.4 mRNA 1438018 1438983 1438018 ID=MS.gene95156.t1;Parent=MS.gene95156
chr8.4 exon 1438018 1438983 1438018 ID=MS.gene95156.t1.exon1;Parent=MS.gene95156.t1
chr8.4 CDS 1438018 1438983 1438018 ID=cds.MS.gene95156.t1;Parent=MS.gene95156.t1
Gene Sequence

>MS.gene95156

ATGAGTATTGCTACAGAACAAGAAACAACATCTCAAGCGTCTCAACAAATTCAAAATCCATCATTTTCCTCTCAAGTTGGGTTAGATAATATTGAGGTTAATGATGAAGAAGAACCAACAGCTCAGATTTCTTCAAAAGAAACAAAAACAAAGGAGAAAATATATAGAGAAAGGTTTACCACGGAGGAAGATACACTTCTTATCCAATCATGGTTAAATATTTCTAAGGATCCAATTGTGGGGGCAAACCAAAGAGCAAGTTCTTTTTGGAAGAGAATTACAGATAATTATAATCAATATCGTGGTCATTTGAAAGGGAGAGAGTTGAATCAAATTAAATGTCGATGGTCGAAGGTGAACTTGATGGTTCATAAATTTGCAACTTGCTACAAACAAGCCTATTCAAGAAAGAAAAGTGGTAGCTCTGAAGACGACATTATGCAAGATGCATTGGCAATTTATGCTCATGATAAGCGTTCACGTTTTATGTTAGTGCCAGCATGGAAATTACTAAAAGATGAACCTAAATGGTCATGCCAATTTACTGAAGAATCCAACAAGAGAACAAAGAATTCTGCTTCAGGAGCCTACACATCCTCTTCTAATCCGGACACGCCTACAAGTGGTGACCCCGGACAATACAACTCAAGTGAAAGTAGAGTGGTTCGTCCGATTGGGACAAAAGCAGCTAAAAGAAAGGGAAAATCAAAAGCAAATGAAACACCTATTGTTCATGTGGATCTAACTAGGATGGAAGATGCACAACAATCAAAAGCTCTAGCTTTAGAAGATATTGCACGTGGTGTGCATGAAGGTAACAAGTTGAAGGAAAAAGAATTGCAAATTAAGGAAAGAAATGCTGAGCTTCACTATCTCTTGATGGATACATCTAGGATGACAGAGGAACAAGTGCAAGCTCATAAAGTGTTGTGTAAAATTATTAGAGAAAAATATGAACTTGTGTAA

Protein sequence

>MS.gene95156.t1

MSIATEQETTSQASQQIQNPSFSSQVGLDNIEVNDEEEPTAQISSKETKTKEKIYRERFTTEEDTLLIQSWLNISKDPIVGANQRASSFWKRITDNYNQYRGHLKGRELNQIKCRWSKVNLMVHKFATCYKQAYSRKKSGSSEDDIMQDALAIYAHDKRSRFMLVPAWKLLKDEPKWSCQFTEESNKRTKNSASGAYTSSSNPDTPTSGDPGQYNSSESRVVRPIGTKAAKRKGKSKANETPIVHVDLTRMEDAQQSKALALEDIARGVHEGNKLKEKELQIKERNAELHYLLMDTSRMTEEQVQAHKVLCKIIREKYELV