Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95519.t1 | XP_003599257.1 | 91.5 | 106 | 9 | 0 | 1 | 106 | 1 | 106 | 8.00E-45 | 189.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95519.t1 | P31336 | 52.3 | 107 | 48 | 2 | 1 | 106 | 1 | 105 | 1.7e-19 | 96.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95519.t1 | G7IWU5 | 91.5 | 106 | 9 | 0 | 1 | 106 | 1 | 106 | 5.7e-45 | 189.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050076 | MS.gene95519 | 0.811951 | 5.41E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene95519 | 0.823354 | 1.45E-53 | -1.69E-46 |
| MS.gene051228 | MS.gene95519 | 0.807494 | 4.92E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene95519 | 0.824089 | 9.75E-54 | -1.69E-46 |
| MS.gene052290 | MS.gene95519 | 0.803621 | 3.19E-49 | -1.69E-46 |
| MS.gene055254 | MS.gene95519 | 0.829395 | 5.27E-55 | -1.69E-46 |
| MS.gene055397 | MS.gene95519 | 0.822134 | 2.79E-53 | -1.69E-46 |
| MS.gene055499 | MS.gene95519 | 0.815422 | 9.32E-52 | -1.69E-46 |
| MS.gene056609 | MS.gene95519 | 0.809778 | 1.60E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene95519 | 0.818761 | 1.66E-52 | -1.69E-46 |
| MS.gene057246 | MS.gene95519 | 0.81292 | 3.33E-51 | -1.69E-46 |
| MS.gene057295 | MS.gene95519 | 0.81575 | 7.88E-52 | -1.69E-46 |
| MS.gene059858 | MS.gene95519 | 0.836044 | 1.18E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95519.t1 | MTR_3g030850 | 91.509 | 106 | 9 | 0 | 1 | 106 | 1 | 106 | 1.88e-64 | 190 |
| MS.gene95519.t1 | MTR_3g030860 | 82.857 | 105 | 17 | 1 | 1 | 105 | 1 | 104 | 2.37e-49 | 152 |
| MS.gene95519.t1 | MTR_4g037505 | 68.224 | 107 | 32 | 2 | 1 | 105 | 1 | 107 | 5.28e-43 | 136 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95519.t1 | AT1G51400 | 57.273 | 110 | 39 | 4 | 1 | 106 | 1 | 106 | 3.17e-28 | 99.0 |
| MS.gene95519.t1 | AT3G21055 | 54.206 | 107 | 42 | 4 | 1 | 105 | 1 | 102 | 1.10e-22 | 85.1 |
| MS.gene95519.t1 | AT3G21055 | 54.206 | 107 | 42 | 4 | 1 | 105 | 41 | 142 | 3.25e-22 | 84.7 |
Find 26 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCTCAATCCTCGGCTTCC+CGG | 0.362856 | 3.4:-24354439 | MS.gene95519:CDS |
| TGACGACGAGGAGTCTCCTC+TGG | 0.437430 | 3.4:+24354388 | None:intergenic |
| AATGCTGTCCGAGCAGTTGA+TGG | 0.443602 | 3.4:-24354366 | MS.gene95519:CDS |
| GTTGGCATAGTGACACAAAC+AGG | 0.491066 | 3.4:+24354183 | None:intergenic |
| GCTTCCCGGCTGTAACCAAC+CGG | 0.507766 | 3.4:-24354425 | MS.gene95519:CDS |
| ATGCTGTCCGAGCAGTTGAT+GGG | 0.515597 | 3.4:-24354365 | MS.gene95519:CDS |
| TCTGGGATGCTACGGATGAC+CGG | 0.520845 | 3.4:+24354406 | None:intergenic |
| AGCCGGGAAGCCGAGGATTG+AGG | 0.531969 | 3.4:+24354437 | None:intergenic |
| GAGTCTCCTCTGGGATGCTA+CGG | 0.538143 | 3.4:+24354398 | None:intergenic |
| GATGACCGGTTGGTTACAGC+CGG | 0.561463 | 3.4:+24354420 | None:intergenic |
| GCCGAGGATTGAGGATGTCA+TGG | 0.565591 | 3.4:+24354446 | None:intergenic |
| TGGTTACAGCCGGGAAGCCG+AGG | 0.566698 | 3.4:+24354430 | None:intergenic |
| GACGACGAGGAGTCTCCTCT+GGG | 0.572413 | 3.4:+24354389 | None:intergenic |
| ACCATGACATCCTCAATCCT+CGG | 0.573002 | 3.4:-24354447 | MS.gene95519:CDS |
| AACAACAAAGAGGGAAGCAA+CGG | 0.574516 | 3.4:-24354321 | MS.gene95519:CDS |
| GTGTCACTATGCCAACAGCT+AGG | 0.576409 | 3.4:-24354176 | MS.gene95519:CDS |
| TTGCGACAAATCCTAGCTGT+TGG | 0.581063 | 3.4:+24354165 | None:intergenic |
| GGATGCTACGGATGACCGGT+TGG | 0.593143 | 3.4:+24354410 | None:intergenic |
| CAAGTATGACAACAACAAAG+AGG | 0.600137 | 3.4:-24354331 | MS.gene95519:CDS |
| TGCTGTCCGAGCAGTTGATG+GGG | 0.601918 | 3.4:-24354364 | MS.gene95519:CDS |
| ACAAAGAGGGAAGCAACGGA+AGG | 0.609771 | 3.4:-24354317 | MS.gene95519:CDS |
| GCAGATGATGAACCTAAAAG+AGG | 0.625646 | 3.4:-24354234 | MS.gene95519:CDS |
| CTCGGACAGCATTGACGACG+AGG | 0.627380 | 3.4:+24354376 | None:intergenic |
| AAGTATGACAACAACAAAGA+GGG | 0.629437 | 3.4:-24354330 | MS.gene95519:CDS |
| ATGACCGGTTGGTTACAGCC+GGG | 0.647755 | 3.4:+24354421 | None:intergenic |
| GGTCATCCGTAGCATCCCAG+AGG | 0.774161 | 3.4:-24354404 | MS.gene95519:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGTATGACAACAACAAAGA+GGG | - | chr3.4:24354290-24354309 | MS.gene95519:CDS | 30.0% | |
| !!! | ATACTTTTTCTTTGCTTCTG+GGG | + | chr3.4:24354414-24354433 | None:intergenic | 30.0% |
| !!! | CATACTTTTTCTTTGCTTCT+GGG | + | chr3.4:24354415-24354434 | None:intergenic | 30.0% |
| CAAGTATGACAACAACAAAG+AGG | - | chr3.4:24354289-24354308 | MS.gene95519:CDS | 35.0% | |
| !!! | GCATACTTTTTCTTTGCTTC+TGG | + | chr3.4:24354416-24354435 | None:intergenic | 35.0% |
| AACAACAAAGAGGGAAGCAA+CGG | - | chr3.4:24354299-24354318 | MS.gene95519:CDS | 40.0% | |
| GCAGATGATGAACCTAAAAG+AGG | - | chr3.4:24354386-24354405 | MS.gene95519:CDS | 40.0% | |
| ACCATGACATCCTCAATCCT+CGG | - | chr3.4:24354173-24354192 | MS.gene95519:CDS | 45.0% | |
| GTTGGCATAGTGACACAAAC+AGG | + | chr3.4:24354440-24354459 | None:intergenic | 45.0% | |
| TTGCGACAAATCCTAGCTGT+TGG | + | chr3.4:24354458-24354477 | None:intergenic | 45.0% | |
| ! | TCTTTTCCCCATCAACTGCT+CGG | + | chr3.4:24354265-24354284 | None:intergenic | 45.0% |
| !!! | CACTTTTGCTGGTGTTGCAA+CGG | - | chr3.4:24354364-24354383 | MS.gene95519:CDS | 45.0% |
| AATGCTGTCCGAGCAGTTGA+TGG | - | chr3.4:24354254-24354273 | MS.gene95519:CDS | 50.0% | |
| ACAAAGAGGGAAGCAACGGA+AGG | - | chr3.4:24354303-24354322 | MS.gene95519:CDS | 50.0% | |
| ATGCTGTCCGAGCAGTTGAT+GGG | - | chr3.4:24354255-24354274 | MS.gene95519:CDS | 50.0% | |
| ! | GTGTCACTATGCCAACAGCT+AGG | - | chr3.4:24354444-24354463 | MS.gene95519:CDS | 50.0% |
| !! | GCTGCTGTTTGCACTTTTGC+TGG | - | chr3.4:24354353-24354372 | MS.gene95519:CDS | 50.0% |
| !!! | GCTTCTGGGGTACCTCTTTT+AGG | + | chr3.4:24354401-24354420 | None:intergenic | 50.0% |
| ATCCTCAATCCTCGGCTTCC+CGG | - | chr3.4:24354181-24354200 | MS.gene95519:CDS | 55.0% | |
| GCCGAGGATTGAGGATGTCA+TGG | + | chr3.4:24354177-24354196 | None:intergenic | 55.0% | |
| ! | ATGACCGGTTGGTTACAGCC+GGG | + | chr3.4:24354202-24354221 | None:intergenic | 55.0% |
| ! | GATGACCGGTTGGTTACAGC+CGG | + | chr3.4:24354203-24354222 | None:intergenic | 55.0% |
| ! | TCTGGGATGCTACGGATGAC+CGG | + | chr3.4:24354217-24354236 | None:intergenic | 55.0% |
| ! | TGCTGTCCGAGCAGTTGATG+GGG | - | chr3.4:24354256-24354275 | MS.gene95519:CDS | 55.0% |
| !! | GAGTCTCCTCTGGGATGCTA+CGG | + | chr3.4:24354225-24354244 | None:intergenic | 55.0% |
| GACGACGAGGAGTCTCCTCT+GGG | + | chr3.4:24354234-24354253 | None:intergenic | 60.0% | |
| GCTTCCCGGCTGTAACCAAC+CGG | - | chr3.4:24354195-24354214 | MS.gene95519:CDS | 60.0% | |
| GGATGCTACGGATGACCGGT+TGG | + | chr3.4:24354213-24354232 | None:intergenic | 60.0% | |
| GGTCATCCGTAGCATCCCAG+AGG | - | chr3.4:24354216-24354235 | MS.gene95519:CDS | 60.0% | |
| TGACGACGAGGAGTCTCCTC+TGG | + | chr3.4:24354235-24354254 | None:intergenic | 60.0% | |
| !! | CTCGGACAGCATTGACGACG+AGG | + | chr3.4:24354247-24354266 | None:intergenic | 60.0% |
| AGCCGGGAAGCCGAGGATTG+AGG | + | chr3.4:24354186-24354205 | None:intergenic | 65.0% | |
| TGGTTACAGCCGGGAAGCCG+AGG | + | chr3.4:24354193-24354212 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 24354161 | 24354481 | 24354161 | ID=MS.gene95519 |
| chr3.4 | mRNA | 24354161 | 24354481 | 24354161 | ID=MS.gene95519.t1;Parent=MS.gene95519 |
| chr3.4 | exon | 24354161 | 24354481 | 24354161 | ID=MS.gene95519.t1.exon1;Parent=MS.gene95519.t1 |
| chr3.4 | CDS | 24354161 | 24354481 | 24354161 | ID=cds.MS.gene95519.t1;Parent=MS.gene95519.t1 |
| Gene Sequence |
| Protein sequence |
