Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95595.t1 | XP_003605958.1 | 100 | 40 | 0 | 0 | 1 | 40 | 103 | 142 | 7.60E-12 | 80.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95595.t1 | G7JEQ0 | 100.0 | 40 | 0 | 0 | 1 | 40 | 103 | 142 | 5.5e-12 | 80.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene95595 | 0.814625 | 1.40E-51 | -1.69E-46 |
| MS.gene04920 | MS.gene95595 | 0.819377 | 1.20E-52 | -1.69E-46 |
| MS.gene049499 | MS.gene95595 | 0.80897 | 2.38E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene95595 | 0.801599 | 8.35E-49 | -1.69E-46 |
| MS.gene053542 | MS.gene95595 | 0.802319 | 5.94E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene95595 | 0.805899 | 1.07E-49 | -1.69E-46 |
| MS.gene058781 | MS.gene95595 | 0.803304 | 3.72E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene95595 | 0.817143 | 3.84E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene95595 | -0.800802 | 1.22E-48 | -1.69E-46 |
| MS.gene060935 | MS.gene95595 | 0.823828 | 1.12E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene95595 | 0.821353 | 4.22E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95595.t1 | MTR_4g049540 | 99.160 | 119 | 1 | 0 | 1 | 119 | 103 | 221 | 4.63e-81 | 237 |
| MS.gene95595.t1 | MTR_2g088060 | 63.559 | 118 | 43 | 0 | 1 | 118 | 104 | 221 | 5.64e-47 | 150 |
| MS.gene95595.t1 | MTR_2g023880 | 56.780 | 118 | 51 | 0 | 1 | 118 | 104 | 221 | 3.57e-41 | 135 |
| MS.gene95595.t1 | MTR_7g056133 | 37.615 | 109 | 61 | 1 | 1 | 102 | 104 | 212 | 5.63e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95595.t1 | AT5G42570 | 71.818 | 110 | 31 | 0 | 1 | 110 | 103 | 212 | 1.07e-49 | 157 |
| MS.gene95595.t1 | AT1G11905 | 52.336 | 107 | 51 | 0 | 1 | 107 | 103 | 209 | 8.57e-30 | 107 |
| MS.gene95595.t1 | AT1G11905 | 50.000 | 102 | 51 | 0 | 6 | 107 | 107 | 208 | 5.94e-26 | 97.1 |
Find 21 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTACCGCATTGAAGTCTA+AGG | 0.333287 | 4.3:-49994653 | MS.gene95595:CDS |
| GCAATCTGAGGGGTTCCTTA+TGG | 0.339577 | 4.3:-49994551 | MS.gene95595:CDS |
| TTTACCATCCTCAAAACTTC+GGG | 0.365140 | 4.3:+49994714 | None:intergenic |
| CTTCAATGCGGTAATTTCTT+CGG | 0.401549 | 4.3:+49994660 | None:intergenic |
| TGGAAGACAATCAGAATCTT+CGG | 0.449833 | 4.3:-49994513 | MS.gene95595:CDS |
| TTGTTTCTTAACAGCTTCCA+TGG | 0.457783 | 4.3:+49994738 | None:intergenic |
| TATGGAATATGATCGCCTCT+TGG | 0.494710 | 4.3:-49994533 | MS.gene95595:CDS |
| AGAGGCGATCATATTCCATA+AGG | 0.520476 | 4.3:+49994536 | None:intergenic |
| TATCAAGATGTGAAGAGCTC+TGG | 0.537183 | 4.3:+49994470 | None:intergenic |
| TAAGAGAGCTTCGTTTACTC+AGG | 0.542022 | 4.3:-49994765 | MS.gene95595:CDS |
| GAATCTGAATGCGAGGCGAA+AGG | 0.544058 | 4.3:-49994619 | MS.gene95595:CDS |
| GACATTGGAAACTGAAGTAG+AGG | 0.553403 | 4.3:-49994584 | MS.gene95595:CDS |
| TCAGAATCTTCGGAGTCAGT+TGG | 0.558726 | 4.3:-49994503 | MS.gene95595:CDS |
| AGGAACCAAGGAAAAGACAT+TGG | 0.565077 | 4.3:-49994599 | MS.gene95595:CDS |
| GAATCTTCGGAGTCAGTTGG+AGG | 0.578067 | 4.3:-49994500 | MS.gene95595:CDS |
| AACGAAGCTCTCTTATGTAG+TGG | 0.615932 | 4.3:+49994773 | None:intergenic |
| TCGTTTACTCAGGAAGACCA+TGG | 0.634666 | 4.3:-49994755 | MS.gene95595:CDS |
| GAAGCTCGAATCTGAATGCG+AGG | 0.644559 | 4.3:-49994626 | MS.gene95595:CDS |
| GATTCTGATTGTCTTCCAAG+AGG | 0.650535 | 4.3:+49994518 | None:intergenic |
| ATGCGAGGCGAAAGGAACCA+AGG | 0.670279 | 4.3:-49994611 | MS.gene95595:CDS |
| CTTAACCTTAGACTTCAATG+CGG | 0.753955 | 4.3:+49994648 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTTACCATCCTCAAAACTT+CGG | + | chr4.3:49994533-49994552 | None:intergenic | 30.0% |
| !!! | AGTTTTGAGGATGGTAAAAA+TGG | - | chr4.3:49994534-49994553 | MS.gene95595:CDS | 30.0% |
| AATTACCGCATTGAAGTCTA+AGG | - | chr4.3:49994590-49994609 | MS.gene95595:CDS | 35.0% | |
| CTCTTAAAAAGCAATCTGAG+GGG | - | chr4.3:49994682-49994701 | MS.gene95595:CDS | 35.0% | |
| CTTAACCTTAGACTTCAATG+CGG | + | chr4.3:49994598-49994617 | None:intergenic | 35.0% | |
| CTTCAATGCGGTAATTTCTT+CGG | + | chr4.3:49994586-49994605 | None:intergenic | 35.0% | |
| GCTCTTAAAAAGCAATCTGA+GGG | - | chr4.3:49994681-49994700 | MS.gene95595:CDS | 35.0% | |
| TGGAAGACAATCAGAATCTT+CGG | - | chr4.3:49994730-49994749 | MS.gene95595:CDS | 35.0% | |
| TTTACCATCCTCAAAACTTC+GGG | + | chr4.3:49994532-49994551 | None:intergenic | 35.0% | |
| ! | AGTTTCCAATGTCTTTTCCT+TGG | + | chr4.3:49994652-49994671 | None:intergenic | 35.0% |
| ! | TTGTTTCTTAACAGCTTCCA+TGG | + | chr4.3:49994508-49994527 | None:intergenic | 35.0% |
| AACGAAGCTCTCTTATGTAG+TGG | + | chr4.3:49994473-49994492 | None:intergenic | 40.0% | |
| AGAGGCGATCATATTCCATA+AGG | + | chr4.3:49994710-49994729 | None:intergenic | 40.0% | |
| AGGAACCAAGGAAAAGACAT+TGG | - | chr4.3:49994644-49994663 | MS.gene95595:CDS | 40.0% | |
| GACATTGGAAACTGAAGTAG+AGG | - | chr4.3:49994659-49994678 | MS.gene95595:CDS | 40.0% | |
| GATTCTGATTGTCTTCCAAG+AGG | + | chr4.3:49994728-49994747 | None:intergenic | 40.0% | |
| GGCTCTTAAAAAGCAATCTG+AGG | - | chr4.3:49994680-49994699 | MS.gene95595:CDS | 40.0% | |
| TAAGAGAGCTTCGTTTACTC+AGG | - | chr4.3:49994478-49994497 | MS.gene95595:CDS | 40.0% | |
| TATCAAGATGTGAAGAGCTC+TGG | + | chr4.3:49994776-49994795 | None:intergenic | 40.0% | |
| TATGGAATATGATCGCCTCT+TGG | - | chr4.3:49994710-49994729 | MS.gene95595:CDS | 40.0% | |
| ! | GAAACAAACCCGAAGTTTTG+AGG | - | chr4.3:49994521-49994540 | MS.gene95595:CDS | 40.0% |
| TCAGAATCTTCGGAGTCAGT+TGG | - | chr4.3:49994740-49994759 | MS.gene95595:CDS | 45.0% | |
| TCGTTTACTCAGGAAGACCA+TGG | - | chr4.3:49994488-49994507 | MS.gene95595:CDS | 45.0% | |
| TGGTAAAAATGGCAGCACAG+AGG | - | chr4.3:49994545-49994564 | MS.gene95595:CDS | 45.0% | |
| !!! | CAAACCCGAAGTTTTGAGGA+TGG | - | chr4.3:49994525-49994544 | MS.gene95595:CDS | 45.0% |
| GAAGCTCGAATCTGAATGCG+AGG | - | chr4.3:49994617-49994636 | MS.gene95595:CDS | 50.0% | |
| GAATCTGAATGCGAGGCGAA+AGG | - | chr4.3:49994624-49994643 | MS.gene95595:CDS | 50.0% | |
| GAATCTTCGGAGTCAGTTGG+AGG | - | chr4.3:49994743-49994762 | MS.gene95595:CDS | 50.0% | |
| GCAATCTGAGGGGTTCCTTA+TGG | - | chr4.3:49994692-49994711 | MS.gene95595:CDS | 50.0% | |
| ATGCGAGGCGAAAGGAACCA+AGG | - | chr4.3:49994632-49994651 | MS.gene95595:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 49994453 | 49994812 | 49994453 | ID=MS.gene95595 |
| chr4.3 | mRNA | 49994453 | 49994812 | 49994453 | ID=MS.gene95595.t1;Parent=MS.gene95595 |
| chr4.3 | exon | 49994453 | 49994812 | 49994453 | ID=MS.gene95595.t1.exon1;Parent=MS.gene95595.t1 |
| chr4.3 | CDS | 49994453 | 49994812 | 49994453 | ID=cds.MS.gene95595.t1;Parent=MS.gene95595.t1 |
| Gene Sequence |
| Protein sequence |
