AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95623

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95623.t1 MTR_7g068910 93.774 514 32 0 3 516 1 514 0.0 1019
MS.gene95623.t1 MTR_7g068910 91.985 524 32 1 3 516 1 524 0.0 1011
MS.gene95623.t1 MTR_4g016920 69.072 485 150 0 32 516 26 510 0.0 732
MS.gene95623.t1 MTR_7g068900 74.402 418 106 1 95 511 17 434 0.0 668
MS.gene95623.t1 MTR_4g016920 68.585 417 131 0 100 516 1 417 0.0 622
MS.gene95623.t1 MTR_1g088610 62.284 464 171 3 34 496 28 488 0.0 611
MS.gene95623.t1 MTR_1g088610 46.703 182 88 2 340 517 458 634 2.08e-41 157
MS.gene95623.t1 MTR_5g068740 60.082 486 189 4 34 517 28 510 0.0 608
MS.gene95623.t1 MTR_1g088630 56.531 467 175 4 52 517 14 453 0.0 543
MS.gene95623.t1 MTR_4g083000 45.051 495 257 5 15 496 10 502 2.67e-160 465
MS.gene95623.t1 MTR_2g098790 48.126 507 245 7 5 497 12 514 1.10e-159 464
MS.gene95623.t1 MTR_2g098520 48.542 480 231 6 32 497 33 510 9.40e-158 459
MS.gene95623.t1 MTR_4g016920 72.632 285 78 0 100 384 1 285 2.59e-157 450
MS.gene95623.t1 MTR_2g098540 46.955 509 254 6 3 497 1 507 2.56e-155 452
MS.gene95623.t1 MTR_3g026400 46.047 506 247 9 11 495 7 507 1.02e-152 446
MS.gene95623.t1 MTR_4g066580 47.401 481 237 6 32 498 38 516 3.30e-152 446
MS.gene95623.t1 MTR_3g026280 47.023 487 245 6 21 497 10 493 3.73e-152 444
MS.gene95623.t1 MTR_6g078450 48.025 481 234 6 32 498 35 513 1.96e-151 443
MS.gene95623.t1 MTR_5g044000 43.969 514 253 7 16 495 8 520 1.40e-149 438
MS.gene95623.t1 MTR_4g066590 46.122 490 248 6 23 498 23 510 2.23e-149 438
MS.gene95623.t1 MTR_6g078380 47.193 481 239 5 32 498 35 514 1.11e-148 436
MS.gene95623.t1 MTR_4g066240 43.836 511 262 7 9 497 5 512 3.63e-148 434
MS.gene95623.t1 MTR_3g028720 44.358 514 262 8 1 498 10 515 7.57e-148 434
MS.gene95623.t1 MTR_3g026620 46.275 510 243 10 12 497 8 510 8.10e-148 434
MS.gene95623.t1 MTR_6g078330 45.010 511 259 8 2 498 12 514 3.78e-147 432
MS.gene95623.t1 MTR_2g079550 44.215 484 255 5 33 504 30 510 8.43e-147 432
MS.gene95623.t1 MTR_2g079580 44.215 484 255 5 33 504 32 512 9.49e-147 431
MS.gene95623.t1 MTR_4g023000 46.138 492 251 7 16 497 7 494 1.36e-146 430
MS.gene95623.t1 MTR_4g066210 45.380 487 234 7 33 497 43 519 1.58e-146 431
MS.gene95623.t1 MTR_1g088630 58.120 351 116 4 35 380 29 353 2.04e-146 425
MS.gene95623.t1 MTR_4g066240 43.750 512 262 8 9 497 5 513 2.06e-146 430
MS.gene95623.t1 MTR_4g066630 45.510 490 248 7 32 507 38 522 3.97e-145 427
MS.gene95623.t1 MTR_4g066640 45.643 482 240 9 32 497 38 513 7.05e-144 424
MS.gene95623.t1 MTR_3g026400 44.948 485 245 8 24 495 1 476 2.00e-143 421
MS.gene95623.t1 MTR_2g098540 47.826 460 224 6 52 497 6 463 4.68e-143 420
MS.gene95623.t1 MTR_5g071520 46.626 489 236 7 32 497 38 524 3.03e-142 420
MS.gene95623.t1 MTR_4g081530 42.998 507 273 7 17 510 8 511 9.62e-140 413
MS.gene95623.t1 MTR_1g088630 69.582 263 77 2 35 296 29 289 3.48e-136 396
MS.gene95623.t1 MTR_3g033810 43.096 478 245 7 32 496 20 483 2.38e-134 399
MS.gene95623.t1 MTR_4g066330 43.776 482 229 7 32 498 40 494 1.62e-133 396
MS.gene95623.t1 MTR_0110s0030 42.739 482 251 6 32 498 42 513 2.19e-133 397
MS.gene95623.t1 MTR_4g066340 46.172 431 217 5 80 497 11 439 2.83e-133 394
MS.gene95623.t1 MTR_2g022710 41.336 479 265 5 32 497 34 509 3.61e-133 396
MS.gene95623.t1 MTR_4g083000 46.098 410 206 5 100 496 1 408 3.73e-132 390
MS.gene95623.t1 MTR_3g033790 42.443 483 250 7 27 496 13 480 7.94e-132 392
MS.gene95623.t1 MTR_4g131810 40.506 474 263 7 38 495 33 503 7.50e-131 390
MS.gene95623.t1 MTR_0110s0040 42.531 482 252 6 32 498 42 513 1.27e-130 390
MS.gene95623.t1 MTR_4g066280 41.200 500 252 7 13 497 21 493 1.97e-130 389
MS.gene95623.t1 MTR_2g022760 42.373 472 255 6 40 497 43 511 2.10e-130 389
MS.gene95623.t1 MTR_4g066580 49.361 391 189 4 32 414 38 427 1.95e-129 384
MS.gene95623.t1 MTR_6g078450 48.317 416 200 5 32 433 35 449 3.41e-128 381
MS.gene95623.t1 MTR_3g028720 46.226 424 211 6 1 414 10 426 3.43e-127 378
MS.gene95623.t1 MTR_3g028720 44.543 449 226 7 1 433 10 451 4.07e-127 379
MS.gene95623.t1 MTR_4g067040 51.989 352 164 3 3 351 6 355 1.10e-126 375
MS.gene95623.t1 MTR_4g067040 51.989 352 164 3 3 351 6 355 2.06e-126 375
MS.gene95623.t1 MTR_4g131800 39.265 517 291 6 1 504 491 997 4.56e-126 393
MS.gene95623.t1 MTR_4g066630 45.913 416 210 5 32 433 38 452 3.34e-125 374
MS.gene95623.t1 MTR_3g026400 49.730 370 172 6 11 370 7 372 4.53e-125 370
MS.gene95623.t1 MTR_4g066630 47.938 388 193 4 32 411 38 424 8.60e-125 372
MS.gene95623.t1 MTR_3g028720 50.000 356 167 4 1 351 10 359 3.00e-123 367
MS.gene95623.t1 MTR_4g066630 50.729 343 165 3 32 371 38 379 6.80e-122 363
MS.gene95623.t1 MTR_4g066240 44.153 419 216 5 9 411 5 421 2.50e-121 363
MS.gene95623.t1 MTR_6g078330 49.858 353 168 4 2 351 12 358 4.39e-121 360
MS.gene95623.t1 MTR_2g079580 45.974 385 201 3 33 411 32 415 9.47e-121 361
MS.gene95623.t1 MTR_6g078330 45.388 412 210 5 100 498 1 410 3.13e-118 355
MS.gene95623.t1 MTR_7g033860 40.592 473 258 9 35 495 50 511 4.68e-116 352
MS.gene95623.t1 MTR_0108s0030 40.909 462 248 7 38 495 8 448 5.69e-116 350
MS.gene95623.t1 MTR_4g066640 51.220 328 153 4 32 355 38 362 1.76e-115 347
MS.gene95623.t1 MTR_2g043200 39.960 503 258 7 12 497 44 519 4.65e-115 350
MS.gene95623.t1 MTR_2g022710 40.898 423 236 4 32 441 34 455 6.40e-115 348
MS.gene95623.t1 MTR_2g022710 40.898 423 236 4 32 441 34 455 1.05e-114 348
MS.gene95623.t1 MTR_4g015460 37.205 508 272 6 11 497 20 501 1.87e-113 345
MS.gene95623.t1 MTR_0110s0040 47.536 345 177 2 32 373 42 385 5.26e-112 338
MS.gene95623.t1 MTR_4g015420 37.450 502 264 7 19 497 51 525 4.21e-111 340
MS.gene95623.t1 MTR_2g022760 41.849 411 223 5 100 497 1 408 4.85e-111 336
MS.gene95623.t1 MTR_2g022710 41.076 409 225 5 100 495 1 406 1.20e-110 335
MS.gene95623.t1 MTR_2g022710 41.344 387 219 3 32 411 34 419 2.57e-108 329
MS.gene95623.t1 MTR_6g078330 44.030 402 208 6 112 500 15 412 1.14e-107 327
MS.gene95623.t1 MTR_7g051650 38.347 472 266 8 34 495 76 532 6.41e-103 319
MS.gene95623.t1 MTR_2g022710 40.909 374 213 3 32 398 34 406 1.16e-102 314
MS.gene95623.t1 MTR_2g022710 42.735 351 199 2 32 381 34 383 3.52e-102 313
MS.gene95623.t1 MTR_7g051510 38.397 474 266 9 34 497 76 533 5.11e-101 314
MS.gene95623.t1 MTR_7g051610 38.784 477 256 10 34 495 76 531 8.45e-101 314
MS.gene95623.t1 MTR_7g051650 37.924 472 264 9 34 495 76 528 1.79e-100 313
MS.gene95623.t1 MTR_7g056747 38.177 406 230 7 94 495 9 397 4.48e-85 269
MS.gene95623.t1 MTR_0110s0020 52.212 226 107 1 33 258 43 267 4.57e-83 260
MS.gene95623.t1 MTR_8g038650 73.810 126 23 1 244 359 35 160 6.88e-58 189
MS.gene95623.t1 MTR_8g038650 96.364 55 2 0 40 94 5 59 4.64e-31 118
MS.gene95623.t1 MTR_7g028885 81.538 65 8 1 241 301 26 90 3.22e-28 108
MS.gene95623.t1 MTR_4g122980 24.691 405 245 11 96 495 183 532 2.38e-20 95.1
MS.gene95623.t1 MTR_5g049830 82.222 45 6 1 300 344 14 56 2.65e-16 73.6
MS.gene95623.t1 MTR_5g049640 82.222 45 6 1 300 344 14 56 2.65e-16 73.6
MS.gene95623.t1 MTR_5g069350 34.426 122 43 4 392 497 89 189 8.02e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95623.t1 AT1G02850 60.981 469 178 3 29 496 22 486 0.0 616
MS.gene95623.t1 AT1G02850 58.130 492 178 4 29 496 22 509 0.0 605
MS.gene95623.t1 AT1G02850 58.012 493 178 5 29 496 22 510 0.0 601
MS.gene95623.t1 AT1G02850 58.422 469 163 4 29 496 22 459 0.0 578
MS.gene95623.t1 AT4G27830 54.209 487 214 5 14 495 2 484 0.0 554
MS.gene95623.t1 AT4G22100 53.265 490 211 6 16 496 3 483 0.0 542
MS.gene95623.t1 AT1G60090 52.559 508 223 9 12 510 1 499 0.0 539
MS.gene95623.t1 AT1G45191 52.286 503 215 8 12 499 1 493 0.0 532
MS.gene95623.t1 AT4G27820 54.604 467 203 3 32 495 22 482 0.0 530
MS.gene95623.t1 AT1G60090 51.351 518 221 10 12 510 1 506 0.0 530
MS.gene95623.t1 AT1G45191 51.575 508 216 8 12 499 1 498 0.0 528
MS.gene95623.t1 AT1G45191 51.697 501 215 8 12 499 1 487 0.0 528
MS.gene95623.t1 AT1G45191 51.362 514 220 9 12 499 1 510 0.0 527
MS.gene95623.t1 AT3G62750 52.381 483 206 7 20 495 8 473 0.0 520
MS.gene95623.t1 AT3G62750 52.381 483 206 7 20 495 34 499 0.0 520
MS.gene95623.t1 AT1G02850 57.740 407 144 4 29 411 22 424 3.42e-174 498
MS.gene95623.t1 AT3G62740 47.904 501 251 5 21 518 9 502 7.98e-171 492
MS.gene95623.t1 AT4G27830 54.271 398 178 2 100 495 1 396 4.96e-160 461
MS.gene95623.t1 AT1G26560 45.912 477 242 6 33 495 32 506 2.04e-156 456
MS.gene95623.t1 AT4G27820 54.386 399 177 2 100 495 1 397 1.77e-154 447
MS.gene95623.t1 AT4G27820 54.386 399 177 2 100 495 1 397 1.77e-154 447
MS.gene95623.t1 AT3G62750 51.740 431 184 7 20 443 34 447 5.99e-154 447
MS.gene95623.t1 AT2G44480 46.025 478 241 7 32 495 34 508 1.34e-147 433
MS.gene95623.t1 AT2G44480 46.025 478 241 7 32 495 65 539 3.55e-147 433
MS.gene95623.t1 AT3G62740 49.289 422 205 4 21 440 9 423 2.51e-146 427
MS.gene95623.t1 AT3G62750 51.372 401 177 5 100 495 1 388 4.23e-144 421
MS.gene95623.t1 AT3G62750 51.372 401 177 5 100 495 1 388 4.23e-144 421
MS.gene95623.t1 AT1G45191 52.972 387 163 6 12 385 1 381 1.43e-143 419
MS.gene95623.t1 AT1G45191 54.032 372 152 6 12 372 1 364 4.00e-143 418
MS.gene95623.t1 AT3G62750 54.622 357 140 5 20 369 8 349 1.19e-141 412
MS.gene95623.t1 AT3G62750 54.622 357 140 5 20 369 8 349 1.19e-141 412
MS.gene95623.t1 AT3G62750 54.444 360 142 5 20 372 34 378 4.76e-141 414
MS.gene95623.t1 AT1G60270 55.000 360 149 5 21 372 6 360 5.88e-141 411
MS.gene95623.t1 AT3G18080 43.243 481 249 5 32 497 38 509 6.58e-139 411
MS.gene95623.t1 AT5G44640 43.849 504 267 8 8 498 4 504 2.79e-136 404
MS.gene95623.t1 AT1G61820 41.650 509 276 8 7 497 1 506 1.83e-135 402
MS.gene95623.t1 AT3G18070 43.750 480 238 7 33 497 30 492 6.40e-134 397
MS.gene95623.t1 AT2G25630 44.016 493 255 8 12 498 9 486 1.59e-133 396
MS.gene95623.t1 AT2G44450 42.857 483 263 6 29 501 27 506 2.63e-133 396
MS.gene95623.t1 AT3G18070 43.750 480 244 7 33 497 30 498 3.20e-133 396
MS.gene95623.t1 AT5G42260 44.255 470 248 7 33 491 31 497 5.72e-133 395
MS.gene95623.t1 AT5G24550 41.717 501 271 8 29 510 28 526 2.79e-131 392
MS.gene95623.t1 AT1G61810 42.316 475 256 7 38 497 38 509 5.38e-131 391
MS.gene95623.t1 AT3G60140 41.833 502 258 9 32 509 25 516 3.77e-130 390
MS.gene95623.t1 AT5G54570 42.029 483 261 8 29 495 26 505 3.86e-129 387
MS.gene95623.t1 AT2G44470 39.155 521 296 9 10 514 6 521 3.55e-128 386
MS.gene95623.t1 AT3G60130 43.579 475 254 7 33 496 30 501 3.11e-127 381
MS.gene95623.t1 AT5G24540 43.117 494 254 10 29 500 28 516 9.56e-125 375
MS.gene95623.t1 AT1G61810 42.174 460 248 7 38 482 38 494 1.72e-124 375
MS.gene95623.t1 AT5G36890 42.292 480 244 8 34 496 17 480 1.99e-123 370
MS.gene95623.t1 AT5G36890 42.292 480 244 8 34 496 17 480 2.04e-123 370
MS.gene95623.t1 AT1G47600 41.286 482 258 10 29 497 41 510 2.33e-122 369
MS.gene95623.t1 AT5G26000 40.984 488 263 8 33 502 38 518 2.42e-122 369
MS.gene95623.t1 AT1G51470 41.408 483 257 10 29 497 41 511 3.22e-122 368
MS.gene95623.t1 AT4G21760 38.197 466 277 5 38 495 59 521 3.94e-122 369
MS.gene95623.t1 AT1G47600 41.201 483 258 10 29 497 41 511 4.45e-122 368
MS.gene95623.t1 AT2G44480 45.232 409 208 7 100 495 1 406 4.73e-122 364
MS.gene95623.t1 AT2G32860 40.000 505 279 11 22 507 84 583 4.03e-120 366
MS.gene95623.t1 AT4G21760 38.696 460 273 6 38 495 59 511 8.14e-120 363
MS.gene95623.t1 AT3G09260 40.246 487 270 9 28 497 30 512 1.97e-118 359
MS.gene95623.t1 AT5G25980 41.408 483 255 11 33 496 50 523 2.93e-118 359
MS.gene95623.t1 AT2G32860 39.921 506 277 12 22 507 84 582 1.12e-117 360
MS.gene95623.t1 AT2G44460 40.354 508 278 9 10 498 6 507 3.76e-117 357
MS.gene95623.t1 AT3G60120 39.388 490 274 9 26 497 7 491 1.09e-116 355
MS.gene95623.t1 AT2G44490 38.492 504 284 10 27 509 8 506 1.10e-116 355
MS.gene95623.t1 AT2G44480 52.632 323 145 5 32 348 65 385 3.64e-116 348
MS.gene95623.t1 AT1G66280 39.286 504 282 10 10 495 13 510 3.20e-115 350
MS.gene95623.t1 AT1G75940 39.876 484 267 10 33 495 37 517 4.54e-115 350
MS.gene95623.t1 AT3G03640 37.665 531 301 13 4 512 5 527 1.23e-113 347
MS.gene95623.t1 AT1G66270 39.959 488 269 10 28 497 31 512 3.26e-113 345
MS.gene95623.t1 AT5G28510 40.365 493 270 10 33 504 39 528 6.61e-113 345
MS.gene95623.t1 AT3G60140 44.133 392 205 5 33 413 26 414 3.11e-112 341
MS.gene95623.t1 AT3G21370 38.945 493 269 10 28 496 28 512 7.91e-112 342
MS.gene95623.t1 AT5G54570 41.355 428 234 6 29 441 26 451 9.32e-112 339
MS.gene95623.t1 AT1G61810 41.990 412 222 6 100 497 1 409 1.65e-110 335
MS.gene95623.t1 AT1G52400 38.956 498 266 11 37 508 43 528 3.75e-110 338
MS.gene95623.t1 AT1G52400 38.956 498 266 11 37 508 43 528 3.75e-110 338
MS.gene95623.t1 AT1G66270 39.549 488 269 11 28 497 31 510 1.39e-109 336
MS.gene95623.t1 AT2G44470 39.013 446 256 6 10 440 6 450 2.05e-109 333
MS.gene95623.t1 AT5G54570 42.336 411 219 7 100 495 1 408 1.32e-108 331
MS.gene95623.t1 AT5G26000 41.549 426 225 7 33 441 38 456 7.31e-108 329
MS.gene95623.t1 AT5G24540 42.459 431 225 7 29 440 28 454 2.98e-106 325
MS.gene95623.t1 AT2G44470 44.058 345 190 2 10 351 6 350 6.66e-106 322
MS.gene95623.t1 AT1G60270 54.945 273 119 3 100 370 1 271 8.60e-106 318
MS.gene95623.t1 AT3G18070 46.866 335 175 2 33 367 30 361 8.69e-106 322
MS.gene95623.t1 AT1G61810 43.117 385 209 5 38 413 38 421 1.00e-105 323
MS.gene95623.t1 AT5G25980 41.315 426 225 9 33 441 50 467 1.26e-102 316
MS.gene95623.t1 AT5G25980 41.315 426 225 9 33 441 50 467 1.26e-102 316
MS.gene95623.t1 AT3G60130 41.422 408 225 6 94 496 40 438 2.84e-102 315
MS.gene95623.t1 AT3G60130 41.404 413 229 6 94 496 40 449 3.36e-102 315
MS.gene95623.t1 AT1G61820 40.327 367 203 5 144 497 4 367 6.70e-92 285
MS.gene95623.t1 AT1G52400 38.861 404 218 8 37 422 43 435 1.52e-88 280
MS.gene95623.t1 AT5G48375 37.757 437 201 9 33 460 38 412 7.15e-87 275
MS.gene95623.t1 AT1G61810 47.191 267 138 3 33 297 33 298 3.17e-84 263
MS.gene95623.t1 AT2G44450 38.268 358 211 4 151 501 1 355 4.52e-83 262
MS.gene95623.t1 AT3G18070 40.950 337 175 6 176 497 94 421 2.11e-78 252
MS.gene95623.t1 AT3G18070 46.032 63 34 0 32 94 29 91 6.69e-11 64.7
MS.gene95623.t1 AT1G51490 42.136 337 170 11 41 369 28 347 1.10e-74 242
MS.gene95623.t1 AT5G16580 46.975 281 136 6 176 451 27 299 4.73e-73 234
MS.gene95623.t1 AT3G18070 40.379 317 165 6 196 497 2 309 5.40e-72 232
MS.gene95623.t1 AT2G44460 40.110 364 201 8 149 498 1 361 9.73e-72 235
MS.gene95623.t1 AT2G44480 39.344 305 171 6 202 495 1 302 3.88e-69 224
MS.gene95623.t1 AT3G18070 40.081 247 130 4 176 411 94 333 4.46e-53 183
MS.gene95623.t1 AT3G18070 46.032 63 34 0 32 94 29 91 4.16e-11 65.1
MS.gene95623.t1 AT3G18070 39.207 227 120 4 196 411 2 221 1.27e-46 162
MS.gene95623.t1 AT3G06510 22.654 437 266 12 95 503 159 551 3.64e-19 91.3
MS.gene95623.t1 AT3G06510 23.934 422 245 12 95 503 159 517 1.65e-17 86.3

Find 112 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTATGCAACATGGATTTAT+TGG 0.188121 7.4:+34653180 MS.gene95623:CDS
GGGTTTAATCTTCTTACTTT+TGG 0.226899 7.4:+34653201 MS.gene95623:CDS
CCAATTTACATACATGAAAA+TGG 0.227583 7.4:+34653975 MS.gene95623:CDS
TAGCACCTCTTGAAAATTCT+TGG 0.231909 7.4:-34653922 None:intergenic
TATTACTTGAAGAATGATTA+TGG 0.244024 7.4:+34653945 MS.gene95623:CDS
TTCTTCAACTGAGCCATATT+TGG 0.244204 7.4:+34652973 MS.gene95623:CDS
TCCTCCATGTTCAGGCTTGC+AGG 0.244360 7.4:-34653627 None:intergenic
GCAATGTTTGGCTATGATAT+TGG 0.252000 7.4:+34652893 MS.gene95623:CDS
TTGTAATATTGTAGTCCTTT+AGG 0.270990 7.4:-34652459 None:intergenic
AAGAGCTCAATTGACTTCTT+AGG 0.273589 7.4:+34653567 MS.gene95623:CDS
ATGGCAGGAAACCAAGTATA+TGG 0.278893 7.4:+34651820 MS.gene95623:CDS
TGAAAATTCTTGGTTGTAAT+CGG 0.280116 7.4:-34653912 None:intergenic
ATCATGGTCAAGACTTATTC+CGG 0.291642 7.4:+34652151 MS.gene95623:CDS
ATATGATGAGCTGCCAAATA+TGG 0.293208 7.4:-34652986 None:intergenic
GTCAATATTTATGCAATGTT+TGG 0.295176 7.4:+34652881 MS.gene95623:CDS
GCAATTCATCCTTGAATGAT+TGG 0.325758 7.4:+34654116 MS.gene95623:CDS
GGATAATCACCAAATGTTAA+AGG 0.331229 7.4:-34653477 None:intergenic
AGGAGTGGATCGAATATAAA+AGG 0.338876 7.4:+34654273 MS.gene95623:intron
TTCCCTTCTGACTTTGTGTT+TGG 0.340012 7.4:+34651441 MS.gene95623:CDS
TTAGGTTGTCTCTTCAAGTT+TGG 0.344000 7.4:-34654384 None:intergenic
AGTGGATATGAAGAAAGTTT+TGG 0.348235 7.4:+34654336 MS.gene95623:CDS
GATGTGTGCTTCAAAGAATT+TGG 0.353470 7.4:+34652824 MS.gene95623:CDS
CACATGTTACATTACATCAT+TGG 0.353709 7.4:+34652595 MS.gene95623:CDS
GTATGCAACATGGATTTATT+GGG 0.354904 7.4:+34653181 MS.gene95623:CDS
GGACCATAGCTAATGAAATA+AGG 0.360367 7.4:+34654170 MS.gene95623:CDS
TTGTAGTCCTTTAGGATTGA+TGG 0.365010 7.4:-34652451 None:intergenic
AACCATAAGCACAATGAGTA+TGG 0.366823 7.4:+34651317 None:intergenic
AACCTAAGCTCTCTGCAAAA+TGG 0.383756 7.4:+34654400 MS.gene95623:CDS
TATGGACTCAAAGATTACTA+TGG 0.386973 7.4:+34654449 MS.gene95623:CDS
CATCCTTGAATGATTGGTCT+AGG 0.400119 7.4:+34654122 MS.gene95623:CDS
CCATGATATTGAAAATCTAT+AGG 0.406574 7.4:-34652135 None:intergenic
AATCTCTATCCTCCATGTTC+AGG 0.412420 7.4:-34653635 None:intergenic
CAATCACTTGAGGATGAGTA+TGG 0.413167 7.4:+34652627 MS.gene95623:CDS
ACATGTTACATTACATCATT+GGG 0.438409 7.4:+34652596 MS.gene95623:CDS
TAGAGATTACATGCAAGATA+TGG 0.438663 7.4:+34653650 MS.gene95623:CDS
AACTATAAGCACTAGGGAAA+AGG 0.440558 7.4:-34651404 None:intergenic
CACCCTAGACCAATCATTCA+AGG 0.449983 7.4:-34654125 None:intergenic
CTTTCATATCTATGTAGTAT+AGG 0.451734 7.4:-34654359 None:intergenic
CATTATAGCAATGATTTACA+TGG 0.453286 7.4:-34654489 None:intergenic
ACCACCCCATCATGAAGTCT+TGG 0.455704 7.4:-34653275 None:intergenic
GTACCTTATTTCATTAGCTA+TGG 0.466073 7.4:-34654173 None:intergenic
ATTGTACAGGAAGAAATATA+AGG 0.468830 7.4:+34653036 MS.gene95623:CDS
CCACCCCATCATGAAGTCTT+GGG 0.477044 7.4:-34653274 None:intergenic
CCTTTGTAGCTAACTGCTCA+AGG 0.482185 7.4:+34652945 MS.gene95623:CDS
TCATGGTCAAGACTTATTCC+GGG 0.483045 7.4:+34652152 MS.gene95623:CDS
ACTTCCTTCCTTCACAGTAA+AGG 0.485769 7.4:+34653530 MS.gene95623:CDS
TGTAGTCCTTTAGGATTGAT+GGG 0.486907 7.4:-34652450 None:intergenic
GCAAAGGTATCCCATATACT+TGG 0.490071 7.4:-34651831 None:intergenic
TAATGGTATTGGAAGTTTGA+AGG 0.498314 7.4:+34651783 MS.gene95623:intron
TTGGCGATCGAGTTAAGTAC+TGG 0.498355 7.4:+34652843 MS.gene95623:CDS
GCAAATATGTACAAAGGTGA+TGG 0.499735 7.4:+34651943 MS.gene95623:intron
AAGATATAATTGCCACTCAA+AGG 0.502064 7.4:+34653250 MS.gene95623:CDS
ATAGTAATCTTTGAGTCCAT+AGG 0.515359 7.4:-34654447 None:intergenic
TGGCAGGAAACCAAGTATAT+GGG 0.518221 7.4:+34651821 MS.gene95623:CDS
AGTACTTGCATAAATACATT+GGG 0.526639 7.4:+34654149 MS.gene95623:CDS
ATCCTTGAATGATTGGTCTA+GGG 0.527356 7.4:+34654123 MS.gene95623:CDS
CCTTGAGCAGTTAGCTACAA+AGG 0.534601 7.4:-34652945 None:intergenic
CAATCCTGCAAGCCTGAACA+TGG 0.535121 7.4:+34653623 MS.gene95623:CDS
ACTGTGAAGGAAGGAAGTCT+TGG 0.536319 7.4:-34653525 None:intergenic
TTGTTCAAACTATAAGCACT+AGG 0.539164 7.4:-34651411 None:intergenic
AGATATAATTGCCACTCAAA+GGG 0.540838 7.4:+34653251 MS.gene95623:CDS
ATTTGATTCCTTTACTGTGA+AGG 0.542853 7.4:-34653538 None:intergenic
GATGTAATGTAACATGTGCT+TGG 0.543711 7.4:-34652590 None:intergenic
AAAGGACCCATCAATCCTAA+AGG 0.554693 7.4:+34652444 MS.gene95623:CDS
GTGCAGCAAATGAAGATGGC+AGG 0.555499 7.4:+34651805 MS.gene95623:CDS
CGATGGATATCACAAATACA+AGG 0.562182 7.4:+34651978 MS.gene95623:CDS
AGGGCCCAAGACTTCATGAT+GGG 0.562999 7.4:+34653270 MS.gene95623:CDS
TGTCCGACTCATGGCCAACA+TGG 0.563885 7.4:+34652103 MS.gene95623:CDS
CTTTGTAGCTAACTGCTCAA+GGG 0.564427 7.4:+34652946 MS.gene95623:CDS
AAGTACTTGCATAAATACAT+TGG 0.567174 7.4:+34654148 MS.gene95623:CDS
TACTCATCCTCAAGTGATTG+TGG 0.568481 7.4:-34652624 None:intergenic
GCAGGAAGATGTCCGACTCA+TGG 0.569087 7.4:+34652094 MS.gene95623:intron
GTACTGGACCACGGTGAATG+AGG 0.570570 7.4:+34652859 MS.gene95623:CDS
GATTCCTTTACTGTGAAGGA+AGG 0.570998 7.4:-34653534 None:intergenic
GAAGTCTTGGGCCCTTTGAG+TGG 0.571906 7.4:-34653262 None:intergenic
GAAGGTGCAGCAAATGAAGA+TGG 0.573414 7.4:+34651801 MS.gene95623:CDS
TGAGGATGAGTATGGAGGAT+GGG 0.579691 7.4:+34652635 MS.gene95623:CDS
GAGCAGTTAGCTACAAAGGA+AGG 0.580100 7.4:-34652941 None:intergenic
ACACCAAACACAAAGTCAGA+AGG 0.580548 7.4:-34651444 None:intergenic
ATGTATTTACCAGATGGTAA+AGG 0.581242 7.4:+34652426 MS.gene95623:intron
ATATGGGATACCTTTGCCCA+TGG 0.593017 7.4:+34651837 MS.gene95623:CDS
TATTTCAAGCACATCCATGA+AGG 0.593077 7.4:-34654310 None:intergenic
TTGAGGATGAGTATGGAGGA+TGG 0.595097 7.4:+34652634 MS.gene95623:CDS
TGTTCAAACTATAAGCACTA+GGG 0.596146 7.4:-34651410 None:intergenic
CTTCTGCTGCAAGATTGTAC+AGG 0.599723 7.4:+34653023 MS.gene95623:CDS
CACCAAACACAAAGTCAGAA+GGG 0.600461 7.4:-34651443 None:intergenic
TATGGGATACCTTTGCCCAT+GGG 0.601326 7.4:+34651838 MS.gene95623:CDS
AGCCCATGTTGGCCATGAGT+CGG 0.602492 7.4:-34652106 None:intergenic
AAGGGCCCAAGACTTCATGA+TGG 0.602550 7.4:+34653269 MS.gene95623:CDS
GTCCGACTCATGGCCAACAT+GGG 0.608501 7.4:+34652104 MS.gene95623:CDS
TTTGTAGCTAACTGCTCAAG+GGG 0.612399 7.4:+34652947 MS.gene95623:CDS
CCCAAGACTTCATGATGGGG+TGG 0.613603 7.4:+34653274 MS.gene95623:CDS
AAAGTGCAGGGTATGCAACA+TGG 0.615675 7.4:+34653171 MS.gene95623:intron
GTTTGGCTATGATATTGGAA+TGG 0.622668 7.4:+34652898 MS.gene95623:CDS
ATGGGATACCTTTGCCCATG+GGG 0.624681 7.4:+34651839 MS.gene95623:CDS
TTACATGCAAGATATGGCAG+TGG 0.628249 7.4:+34653656 MS.gene95623:CDS
TTGGAATGGCACCGCCTCAG+AGG 0.629344 7.4:+34652912 MS.gene95623:CDS
ATAAATGAACTGATAAGCCA+CGG 0.631869 7.4:+34652489 MS.gene95623:CDS
GATGGAGATATTGCATGCGA+TGG 0.633887 7.4:+34651961 MS.gene95623:CDS
AAATGATACATCAATAGATG+AGG 0.637241 7.4:+34653801 MS.gene95623:CDS
GGATCTACCACAATCACTTG+AGG 0.639081 7.4:+34652617 MS.gene95623:CDS
TCTTCACTAGTATTTGTCAG+TGG 0.645429 7.4:-34653231 None:intergenic
GAAGGAGAACACCTCTGAGG+CGG 0.652236 7.4:-34652923 None:intergenic
ATAGGCATCTAAGCCCATGT+TGG 0.654884 7.4:-34652117 None:intergenic
TGGGATACCTTTGCCCATGG+GGG 0.675501 7.4:+34651840 MS.gene95623:CDS
TCACTTGAGGATGAGTATGG+AGG 0.676589 7.4:+34652630 MS.gene95623:CDS
GGGCCCAAGACTTCATGATG+GGG 0.691282 7.4:+34653271 MS.gene95623:CDS
TCCTGCAAGCCTGAACATGG+AGG 0.718680 7.4:+34653626 MS.gene95623:CDS
AAGGAAGGAGAACACCTCTG+AGG 0.733690 7.4:-34652926 None:intergenic
TCGAGTTAAGTACTGGACCA+CGG 0.736198 7.4:+34652850 MS.gene95623:CDS
GATGTGCTTGAAATACTGAG+TGG 0.749862 7.4:+34654318 MS.gene95623:CDS
AATATTGACCTCATTCACCG+TGG 0.768238 7.4:-34652867 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTAATTTATTTATGTATT+TGG + chr7.4:34653856-34653875 MS.gene95623:intron 10.0%
!! AAATACACAAAATTAAGAAA+TGG + chr7.4:34652738-34652757 MS.gene95623:intron 15.0%
!! ATACATAAATAAATTACAAG+AGG - chr7.4:34653855-34653874 None:intergenic 15.0%
!! ATATATGTTTGTTTACAATA+AGG - chr7.4:34651679-34651698 None:intergenic 15.0%
!! ATGTATTACAAAATAAATCT+TGG - chr7.4:34653378-34653397 None:intergenic 15.0%
!! ATTGTCAAATATAATGTATA+AGG + chr7.4:34654206-34654225 MS.gene95623:intron 15.0%
!!! ATATAAAAGGTTATTTTGTA+TGG + chr7.4:34654286-34654305 MS.gene95623:CDS 15.0%
!! AAATTAGATTGTAAGTAAAG+TGG - chr7.4:34653085-34653104 None:intergenic 20.0%
!! ATTTCTAAACTTTGCATTAT+GGG + chr7.4:34653344-34653363 MS.gene95623:intron 20.0%
!! CATTATACTTATGACTATAA+TGG + chr7.4:34651766-34651785 MS.gene95623:intron 20.0%
!! GATATAATGAAAAAGAATGT+TGG + chr7.4:34653501-34653520 MS.gene95623:CDS 20.0%
!! TATTACTTGAAGAATGATTA+TGG + chr7.4:34653945-34653964 MS.gene95623:CDS 20.0%
!! TCTAATATATTTCATCTTCT+AGG + chr7.4:34652564-34652583 MS.gene95623:intron 20.0%
!! TTATTTCAATCTATGACAAT+AGG - chr7.4:34652713-34652732 None:intergenic 20.0%
!!! AATATTGTGAAGATTTTGAA+AGG - chr7.4:34654042-34654061 None:intergenic 20.0%
!!! ACATTCTTTTTCATTATATC+AGG - chr7.4:34653501-34653520 None:intergenic 20.0%
!!! GTTTTAAATCCTTTAACATT+TGG + chr7.4:34653468-34653487 MS.gene95623:CDS 20.0%
!!! TTAAGAAATGGTTTTTATTG+TGG + chr7.4:34652750-34652769 MS.gene95623:intron 20.0%
! AAATGATACATCAATAGATG+AGG + chr7.4:34653801-34653820 MS.gene95623:CDS 25.0%
! AAGTACTTGCATAAATACAT+TGG + chr7.4:34654148-34654167 MS.gene95623:CDS 25.0%
! ACATGTTACATTACATCATT+GGG + chr7.4:34652596-34652615 MS.gene95623:CDS 25.0%
! ACTTATGACTATAATGGTAT+TGG + chr7.4:34651772-34651791 MS.gene95623:intron 25.0%
! AGTACTTGCATAAATACATT+GGG + chr7.4:34654149-34654168 MS.gene95623:CDS 25.0%
! ATTGTACAGGAAGAAATATA+AGG + chr7.4:34653036-34653055 MS.gene95623:CDS 25.0%
! CATTTCTAAACTTTGCATTA+TGG + chr7.4:34653343-34653362 MS.gene95623:intron 25.0%
! CCAATTTACATACATGAAAA+TGG + chr7.4:34653975-34653994 MS.gene95623:CDS 25.0%
! CCATGATATTGAAAATCTAT+AGG - chr7.4:34652138-34652157 None:intergenic 25.0%
! CTTTCATATCTATGTAGTAT+AGG - chr7.4:34654362-34654381 None:intergenic 25.0%
! GTACAATGTGTTATATCATT+TGG - chr7.4:34651567-34651586 None:intergenic 25.0%
! GTCAATATTTATGCAATGTT+TGG + chr7.4:34652881-34652900 MS.gene95623:CDS 25.0%
! GTTAGCAAAAAAATTGTGTA+TGG + chr7.4:34652657-34652676 MS.gene95623:intron 25.0%
! TGAAAATTCTTGGTTGTAAT+CGG - chr7.4:34653915-34653934 None:intergenic 25.0%
! TGAAGTAGTATATGTTAGTT+TGG - chr7.4:34651874-34651893 None:intergenic 25.0%
! TTGTAATATTGTAGTCCTTT+AGG - chr7.4:34652462-34652481 None:intergenic 25.0%
! TTGTTTCAGTTTCTTCAAAA+TGG - chr7.4:34653745-34653764 None:intergenic 25.0%
! TTTCGATGTGTCAAAAATAA+AGG - chr7.4:34654254-34654273 None:intergenic 25.0%
!! AAATGCAAAAGCATAACATT+AGG - chr7.4:34651519-34651538 None:intergenic 25.0%
!! CCATTTTCATGTATGTAAAT+TGG - chr7.4:34653978-34653997 None:intergenic 25.0%
!! CCTATAGATTTTCAATATCA+TGG + chr7.4:34652135-34652154 MS.gene95623:CDS 25.0%
!! TATGACAATTTTCCAACATA+TGG + chr7.4:34653998-34654017 MS.gene95623:intron 25.0%
!! TTTTCAGACTATAATAAGTC+TGG + chr7.4:34651624-34651643 MS.gene95623:intron 25.0%
!! TTTTCAGCAAATATGTACAA+AGG + chr7.4:34651937-34651956 MS.gene95623:intron 25.0%
!!! GTTTATCATTTGTTATTGCA+GGG + chr7.4:34653446-34653465 MS.gene95623:intron 25.0%
!!! TGTTTATCATTTGTTATTGC+AGG + chr7.4:34653445-34653464 MS.gene95623:intron 25.0%
!!! TTTATTTTTGACACATCGAA+AGG + chr7.4:34654253-34654272 MS.gene95623:intron 25.0%
AAAAGAGAGAAGAACAAACT+TGG - chr7.4:34651485-34651504 None:intergenic 30.0%
AAGATATAATTGCCACTCAA+AGG + chr7.4:34653250-34653269 MS.gene95623:CDS 30.0%
AATAGGTAACTCAAACAGAA+TGG - chr7.4:34652696-34652715 None:intergenic 30.0%
AGATATAATTGCCACTCAAA+GGG + chr7.4:34653251-34653270 MS.gene95623:CDS 30.0%
ATAAATGAACTGATAAGCCA+CGG + chr7.4:34652489-34652508 MS.gene95623:CDS 30.0%
ATAGGTAACTCAAACAGAAT+GGG - chr7.4:34652695-34652714 None:intergenic 30.0%
ATAGTAATCTTTGAGTCCAT+AGG - chr7.4:34654450-34654469 None:intergenic 30.0%
ATCTATATATGTATCCTTGC+AGG + chr7.4:34652780-34652799 MS.gene95623:intron 30.0%
ATGTATTTACCAGATGGTAA+AGG + chr7.4:34652426-34652445 MS.gene95623:intron 30.0%
ATTTGATTCCTTTACTGTGA+AGG - chr7.4:34653541-34653560 None:intergenic 30.0%
CACATGTTACATTACATCAT+TGG + chr7.4:34652595-34652614 MS.gene95623:CDS 30.0%
GGATAATCACCAAATGTTAA+AGG - chr7.4:34653480-34653499 None:intergenic 30.0%
GTATGCAACATGGATTTATT+GGG + chr7.4:34653181-34653200 MS.gene95623:CDS 30.0%
TACCATCTGGTAAATACATA+GGG - chr7.4:34652425-34652444 None:intergenic 30.0%
TAGAGATTACATGCAAGATA+TGG + chr7.4:34653650-34653669 MS.gene95623:CDS 30.0%
TATGGACTCAAAGATTACTA+TGG + chr7.4:34654449-34654468 MS.gene95623:CDS 30.0%
TGTTCAAACTATAAGCACTA+GGG - chr7.4:34651413-34651432 None:intergenic 30.0%
TTACCATCTGGTAAATACAT+AGG - chr7.4:34652426-34652445 None:intergenic 30.0%
TTCTTCAAAATGGAGTAAAG+TGG - chr7.4:34653735-34653754 None:intergenic 30.0%
TTGTTCAAACTATAAGCACT+AGG - chr7.4:34651414-34651433 None:intergenic 30.0%
! AGTGGATATGAAGAAAGTTT+TGG + chr7.4:34654336-34654355 MS.gene95623:CDS 30.0%
! GGGTTTAATCTTCTTACTTT+TGG + chr7.4:34653201-34653220 MS.gene95623:CDS 30.0%
! GTACCTTATTTCATTAGCTA+TGG - chr7.4:34654176-34654195 None:intergenic 30.0%
! TAATGGTATTGGAAGTTTGA+AGG + chr7.4:34651783-34651802 MS.gene95623:intron 30.0%
!! AAACAATCTTTCTAAAGTGC+AGG + chr7.4:34653158-34653177 MS.gene95623:intron 30.0%
!! AACAATCTTTCTAAAGTGCA+GGG + chr7.4:34653159-34653178 MS.gene95623:intron 30.0%
!! GAAGATTTTGAAAGGAAACA+TGG - chr7.4:34654034-34654053 None:intergenic 30.0%
!! GGTACTCAAATTTTCTGAAA+AGG + chr7.4:34654421-34654440 MS.gene95623:CDS 30.0%
!! TACAACCAAGAATTTTCAAG+AGG + chr7.4:34653917-34653936 MS.gene95623:CDS 30.0%
!! TTATTTTGTATGGTCCTTCA+TGG + chr7.4:34654296-34654315 MS.gene95623:CDS 30.0%
AAACCAACCAGATGATCAAT+TGG - chr7.4:34653887-34653906 None:intergenic 35.0%
AACTATAAGCACTAGGGAAA+AGG - chr7.4:34651407-34651426 None:intergenic 35.0%
AATATAGTCTTCACAAACCG+TGG - chr7.4:34652509-34652528 None:intergenic 35.0%
AGGAGTGGATCGAATATAAA+AGG + chr7.4:34654273-34654292 MS.gene95623:intron 35.0%
ATAGGGATAACTGGATAAGA+TGG - chr7.4:34652408-34652427 None:intergenic 35.0%
ATATGATGAGCTGCCAAATA+TGG - chr7.4:34652989-34653008 None:intergenic 35.0%
ATCATGGTCAAGACTTATTC+CGG + chr7.4:34652151-34652170 MS.gene95623:CDS 35.0%
ATCCCTATGTATTTACCAGA+TGG + chr7.4:34652420-34652439 MS.gene95623:intron 35.0%
CAAACAGAATGGGAAAGTTT+GGG - chr7.4:34652685-34652704 None:intergenic 35.0%
CGATGGATATCACAAATACA+AGG + chr7.4:34651978-34651997 MS.gene95623:CDS 35.0%
GATGTAATGTAACATGTGCT+TGG - chr7.4:34652593-34652612 None:intergenic 35.0%
GATGTGTGCTTCAAAGAATT+TGG + chr7.4:34652824-34652843 MS.gene95623:CDS 35.0%
GCAAATATGTACAAAGGTGA+TGG + chr7.4:34651943-34651962 MS.gene95623:intron 35.0%
GCAATGTTTGGCTATGATAT+TGG + chr7.4:34652893-34652912 MS.gene95623:CDS 35.0%
GCAATTCATCCTTGAATGAT+TGG + chr7.4:34654116-34654135 MS.gene95623:CDS 35.0%
GGACCATAGCTAATGAAATA+AGG + chr7.4:34654170-34654189 MS.gene95623:CDS 35.0%
GGTAAATACATAGGGATAAC+TGG - chr7.4:34652417-34652436 None:intergenic 35.0%
GGTATGCAACATGGATTTAT+TGG + chr7.4:34653180-34653199 MS.gene95623:CDS 35.0%
GGTTTAACCAATTGATCATC+TGG + chr7.4:34653877-34653896 MS.gene95623:intron 35.0%
GTTTGGCTATGATATTGGAA+TGG + chr7.4:34652898-34652917 MS.gene95623:CDS 35.0%
TAACCAATTGATCATCTGGT+TGG + chr7.4:34653881-34653900 MS.gene95623:intron 35.0%
TAGCACCTCTTGAAAATTCT+TGG - chr7.4:34653925-34653944 None:intergenic 35.0%
TATTTCAAGCACATCCATGA+AGG - chr7.4:34654313-34654332 None:intergenic 35.0%
TCAAACAGAATGGGAAAGTT+TGG - chr7.4:34652686-34652705 None:intergenic 35.0%
TCTAAACTTTGCATTATGGG+TGG + chr7.4:34653347-34653366 MS.gene95623:intron 35.0%
TCTTAGAGGGAATTTGAACT+CGG + chr7.4:34652216-34652235 MS.gene95623:intron 35.0%
TCTTCACTAGTATTTGTCAG+TGG - chr7.4:34653234-34653253 None:intergenic 35.0%
TGTATTCGACTCACTTGATT+TGG + chr7.4:34652305-34652324 MS.gene95623:intron 35.0%
TTCTTCAACTGAGCCATATT+TGG + chr7.4:34652973-34652992 MS.gene95623:CDS 35.0%
! AAGAGCTCAATTGACTTCTT+AGG + chr7.4:34653567-34653586 MS.gene95623:CDS 35.0%
! CATTTTACCACTCTCTTAGA+GGG + chr7.4:34652203-34652222 MS.gene95623:intron 35.0%
! TTAGGTTGTCTCTTCAAGTT+TGG - chr7.4:34654387-34654406 None:intergenic 35.0%
!! ATCCTTGAATGATTGGTCTA+GGG + chr7.4:34654123-34654142 MS.gene95623:CDS 35.0%
!! TGTAGTCCTTTAGGATTGAT+GGG - chr7.4:34652453-34652472 None:intergenic 35.0%
!! TTGTAGTCCTTTAGGATTGA+TGG - chr7.4:34652454-34652473 None:intergenic 35.0%
!! TTTCAGATCTTAGTCTTACC+CGG - chr7.4:34652173-34652192 None:intergenic 35.0%
!! TTTTCTGAAAAGGAAGCCTA+TGG + chr7.4:34654431-34654450 MS.gene95623:CDS 35.0%
AAAGGACCCATCAATCCTAA+AGG + chr7.4:34652444-34652463 MS.gene95623:CDS 40.0%
AACCTAAGCTCTCTGCAAAA+TGG + chr7.4:34654400-34654419 MS.gene95623:CDS 40.0%
AATATTGACCTCATTCACCG+TGG - chr7.4:34652870-34652889 None:intergenic 40.0%
AATCTCTATCCTCCATGTTC+AGG - chr7.4:34653638-34653657 None:intergenic 40.0%
ACACCAAACACAAAGTCAGA+AGG - chr7.4:34651447-34651466 None:intergenic 40.0%
ACTTCCTTCCTTCACAGTAA+AGG + chr7.4:34653530-34653549 MS.gene95623:CDS 40.0%
ATGGCAGGAAACCAAGTATA+TGG + chr7.4:34651820-34651839 MS.gene95623:CDS 40.0%
CAATCACTTGAGGATGAGTA+TGG + chr7.4:34652627-34652646 MS.gene95623:CDS 40.0%
CACCAAACACAAAGTCAGAA+GGG - chr7.4:34651446-34651465 None:intergenic 40.0%
CTTTGTAGCTAACTGCTCAA+GGG + chr7.4:34652946-34652965 MS.gene95623:CDS 40.0%
GATGTGAAATCTTTCCTGCA+AGG - chr7.4:34652797-34652816 None:intergenic 40.0%
GATGTGCTTGAAATACTGAG+TGG + chr7.4:34654318-34654337 MS.gene95623:CDS 40.0%
GATTCCTTTACTGTGAAGGA+AGG - chr7.4:34653537-34653556 None:intergenic 40.0%
GGAATGATCATGCCATATGT+TGG - chr7.4:34654013-34654032 None:intergenic 40.0%
GGTAACATAGTGCAGTAACA+AGG + chr7.4:34653822-34653841 MS.gene95623:intron 40.0%
TACTCATCCTCAAGTGATTG+TGG - chr7.4:34652627-34652646 None:intergenic 40.0%
TCATGGTCAAGACTTATTCC+GGG + chr7.4:34652152-34652171 MS.gene95623:CDS 40.0%
TGGCAGGAAACCAAGTATAT+GGG + chr7.4:34651821-34651840 MS.gene95623:CDS 40.0%
TTACATGCAAGATATGGCAG+TGG + chr7.4:34653656-34653675 MS.gene95623:CDS 40.0%
TTCCCTTCTGACTTTGTGTT+TGG + chr7.4:34651441-34651460 MS.gene95623:CDS 40.0%
TTTGTAGCTAACTGCTCAAG+GGG + chr7.4:34652947-34652966 MS.gene95623:CDS 40.0%
! ATCGAGGTCTTGTATTTGCT+TGG - chr7.4:34652067-34652086 None:intergenic 40.0%
! CCATTTTACCACTCTCTTAG+AGG + chr7.4:34652202-34652221 MS.gene95623:intron 40.0%
! CCTCTAAGAGAGTGGTAAAA+TGG - chr7.4:34652205-34652224 None:intergenic 40.0%
! TACCATTTTGCAGAGAGCTT+AGG - chr7.4:34654405-34654424 None:intergenic 40.0%
! TCAAATTCCCTCTAAGAGAG+TGG - chr7.4:34652213-34652232 None:intergenic 40.0%
! TTTTGACACATCGAAAGGAG+TGG + chr7.4:34654258-34654277 MS.gene95623:intron 40.0%
!! CATCCTTGAATGATTGGTCT+AGG + chr7.4:34654122-34654141 MS.gene95623:CDS 40.0%
!! GCAAAGGTATCCCATATACT+TGG - chr7.4:34651834-34651853 None:intergenic 40.0%
AAAGTGCAGGGTATGCAACA+TGG + chr7.4:34653171-34653190 MS.gene95623:intron 45.0%
ACTGTGAAGGAAGGAAGTCT+TGG - chr7.4:34653528-34653547 None:intergenic 45.0%
AGCCTGCAAAATGAACTCGA+AGG - chr7.4:34654084-34654103 None:intergenic 45.0%
ATAGGCATCTAAGCCCATGT+TGG - chr7.4:34652120-34652139 None:intergenic 45.0%
ATATGGGATACCTTTGCCCA+TGG + chr7.4:34651837-34651856 MS.gene95623:CDS 45.0%
CACCCTAGACCAATCATTCA+AGG - chr7.4:34654128-34654147 None:intergenic 45.0%
CCTTGAGCAGTTAGCTACAA+AGG - chr7.4:34652948-34652967 None:intergenic 45.0%
CCTTTGTAGCTAACTGCTCA+AGG + chr7.4:34652945-34652964 MS.gene95623:CDS 45.0%
CTTCTGCTGCAAGATTGTAC+AGG + chr7.4:34653023-34653042 MS.gene95623:CDS 45.0%
GAGCAGTTAGCTACAAAGGA+AGG - chr7.4:34652944-34652963 None:intergenic 45.0%
GATGGAGATATTGCATGCGA+TGG + chr7.4:34651961-34651980 MS.gene95623:CDS 45.0%
GGATCTACCACAATCACTTG+AGG + chr7.4:34652617-34652636 MS.gene95623:CDS 45.0%
TATGGGATACCTTTGCCCAT+GGG + chr7.4:34651838-34651857 MS.gene95623:CDS 45.0%
TCACTTGAGGATGAGTATGG+AGG + chr7.4:34652630-34652649 MS.gene95623:CDS 45.0%
TCTTCCTGCAGGAAAAATCG+AGG - chr7.4:34652083-34652102 None:intergenic 45.0%
TGAGGATGAGTATGGAGGAT+GGG + chr7.4:34652635-34652654 MS.gene95623:CDS 45.0%
TTGAGGATGAGTATGGAGGA+TGG + chr7.4:34652634-34652653 MS.gene95623:CDS 45.0%
TTGGCGATCGAGTTAAGTAC+TGG + chr7.4:34652843-34652862 MS.gene95623:CDS 45.0%
TTTGGTACCATTTCCCCCAT+GGG - chr7.4:34651856-34651875 None:intergenic 45.0%
! AAGACCTCGATTTTTCCTGC+AGG + chr7.4:34652076-34652095 MS.gene95623:intron 45.0%
! GAAGGTGCAGCAAATGAAGA+TGG + chr7.4:34651801-34651820 MS.gene95623:CDS 45.0%
! TTGATGGGTCCTTTACCATC+TGG - chr7.4:34652438-34652457 None:intergenic 45.0%
!! AGCCTTCGAGTTCATTTTGC+AGG + chr7.4:34654079-34654098 MS.gene95623:intron 45.0%
!! TCGAGTTAAGTACTGGACCA+CGG + chr7.4:34652850-34652869 MS.gene95623:CDS 45.0%
AAGGAAGGAGAACACCTCTG+AGG - chr7.4:34652929-34652948 None:intergenic 50.0%
AAGGGCCCAAGACTTCATGA+TGG + chr7.4:34653269-34653288 MS.gene95623:CDS 50.0%
ACCACCCCATCATGAAGTCT+TGG - chr7.4:34653278-34653297 None:intergenic 50.0%
AGGGCCCAAGACTTCATGAT+GGG + chr7.4:34653270-34653289 MS.gene95623:CDS 50.0%
ATGGGATACCTTTGCCCATG+GGG + chr7.4:34651839-34651858 MS.gene95623:CDS 50.0%
CAATCCTGCAAGCCTGAACA+TGG + chr7.4:34653623-34653642 MS.gene95623:CDS 50.0%
CCACCCCATCATGAAGTCTT+GGG - chr7.4:34653277-34653296 None:intergenic 50.0%
GTGCAGCAAATGAAGATGGC+AGG + chr7.4:34651805-34651824 MS.gene95623:CDS 50.0%
GTTTGGTACCATTTCCCCCA+TGG - chr7.4:34651857-34651876 None:intergenic 50.0%
ACCATTTCCCCCATGGGCAA+AGG - chr7.4:34651850-34651869 None:intergenic 55.0%
ACCTTTGCCCATGGGGGAAA+TGG + chr7.4:34651846-34651865 MS.gene95623:CDS 55.0%
AGCCCATGTTGGCCATGAGT+CGG - chr7.4:34652109-34652128 None:intergenic 55.0%
GAAGTCTTGGGCCCTTTGAG+TGG - chr7.4:34653265-34653284 None:intergenic 55.0%
GCAGGAAGATGTCCGACTCA+TGG + chr7.4:34652094-34652113 MS.gene95623:intron 55.0%
GGGCCCAAGACTTCATGATG+GGG + chr7.4:34653271-34653290 MS.gene95623:CDS 55.0%
GTACTGGACCACGGTGAATG+AGG + chr7.4:34652859-34652878 MS.gene95623:CDS 55.0%
GTCCGACTCATGGCCAACAT+GGG + chr7.4:34652104-34652123 MS.gene95623:CDS 55.0%
TCCTCCATGTTCAGGCTTGC+AGG - chr7.4:34653630-34653649 None:intergenic 55.0%
TCCTGCAAGCCTGAACATGG+AGG + chr7.4:34653626-34653645 MS.gene95623:CDS 55.0%
TGAGTCGGACATCTTCCTGC+AGG - chr7.4:34652094-34652113 None:intergenic 55.0%
TGGGATACCTTTGCCCATGG+GGG + chr7.4:34651840-34651859 MS.gene95623:CDS 55.0%
TGTCCGACTCATGGCCAACA+TGG + chr7.4:34652103-34652122 MS.gene95623:CDS 55.0%
! CCCAAGACTTCATGATGGGG+TGG + chr7.4:34653274-34653293 MS.gene95623:CDS 55.0%
! GAAGGAGAACACCTCTGAGG+CGG - chr7.4:34652926-34652945 None:intergenic 55.0%
TTGGAATGGCACCGCCTCAG+AGG + chr7.4:34652912-34652931 MS.gene95623:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 34651330 34654509 34651330 ID=MS.gene95623
chr7.4 mRNA 34651330 34654509 34651330 ID=MS.gene95623.t1;Parent=MS.gene95623
chr7.4 exon 34651330 34651485 34651330 ID=MS.gene95623.t1.exon1;Parent=MS.gene95623.t1
chr7.4 CDS 34651330 34651485 34651330 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34651798 34651867 34651798 ID=MS.gene95623.t1.exon2;Parent=MS.gene95623.t1
chr7.4 CDS 34651798 34651867 34651798 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34651944 34651999 34651944 ID=MS.gene95623.t1.exon3;Parent=MS.gene95623.t1
chr7.4 CDS 34651944 34651999 34651944 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34652098 34652173 34652098 ID=MS.gene95623.t1.exon4;Parent=MS.gene95623.t1
chr7.4 CDS 34652098 34652173 34652098 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34652439 34652510 34652439 ID=MS.gene95623.t1.exon5;Parent=MS.gene95623.t1
chr7.4 CDS 34652439 34652510 34652439 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34652586 34652673 34652586 ID=MS.gene95623.t1.exon6;Parent=MS.gene95623.t1
chr7.4 CDS 34652586 34652673 34652586 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34652802 34653057 34652802 ID=MS.gene95623.t1.exon7;Parent=MS.gene95623.t1
chr7.4 CDS 34652802 34653057 34652802 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34653180 34653295 34653180 ID=MS.gene95623.t1.exon8;Parent=MS.gene95623.t1
chr7.4 CDS 34653180 34653295 34653180 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34653467 34653687 34653467 ID=MS.gene95623.t1.exon9;Parent=MS.gene95623.t1
chr7.4 CDS 34653467 34653687 34653467 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34653791 34653822 34653791 ID=MS.gene95623.t1.exon10;Parent=MS.gene95623.t1
chr7.4 CDS 34653791 34653822 34653791 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34653909 34653996 34653909 ID=MS.gene95623.t1.exon11;Parent=MS.gene95623.t1
chr7.4 CDS 34653909 34653996 34653909 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34654101 34654191 34654101 ID=MS.gene95623.t1.exon12;Parent=MS.gene95623.t1
chr7.4 CDS 34654101 34654191 34654101 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
chr7.4 exon 34654275 34654509 34654275 ID=MS.gene95623.t1.exon13;Parent=MS.gene95623.t1
chr7.4 CDS 34654275 34654509 34654275 ID=cds.MS.gene95623.t1;Parent=MS.gene95623.t1
Gene Sequence

>MS.gene95623

ATGAGTATGGCTTTGATCTTGAAGAATTTTTCTTTGACAGAATTATTAACTGCTGCAGCAGTGTTAGTAGTAATCCTTTTCCCTAGTGCTTATAGTTTGAACAAAGATGACTTCCCTTCTGACTTTGTGTTTGGTGTATCAACTTCAGCTTACCAAGTTTGTTCTTCTCTCTTTTTTTTTTCAATTCCTAATGTTATGCTTTTGCATTTTCTTTATATTCATGTATGATTATTTCCAAATGATATAACACATTGTACTAGAACATTCTTTAGTGAAAAGAGTTAGAGCATGTTCTTTTCAGACTATAATAAGTCTGGTCAATGAAAAAGAATGTTCAATAAGTAATCCTTATTGTAAACAAACATATATTACTTTAAGCTTTCTGCAAAGATCTTTATATTTTTAATCTTAAACAATCATATATACTCTTAATCTTCATTATACTTATGACTATAATGGTATTGGAAGTTTGAAGGTGCAGCAAATGAAGATGGCAGGAAACCAAGTATATGGGATACCTTTGCCCATGGGGGAAATGGTACCAAACTAACATATACTACTTCATTCTTAATGTTTTGTTGAAACAAAAGTTTATAGTCAAAGTATATTTTCAGCAAATATGTACAAAGGTGATGGAGATATTGCATGCGATGGATATCACAAATACAAGGTCAGTGATATGCATCTTATACATAAAAATACTTTATTTTGAAGTTCATCATCTAAAAATTGCACCAAGCAAATACAAGACCTCGATTTTTCCTGCAGGAAGATGTCCGACTCATGGCCAACATGGGCTTAGATGCCTATAGATTTTCAATATCATGGTCAAGACTTATTCCGGGTAAGACTAAGATCTGAAATGATACTAACCATTTTACCACTCTCTTAGAGGGAATTTGAACTCGGTCATTTGAAGTTGAAAAATCAAACTCTTACTATTGAACTAATATGTCAAGACTAAAAAAAGTTTTTTGTATTCGACTCACTTGATTTGGTTTAAGTGCATAGATCTTTGATGTTATCTGAATATAGATTCATAGATTAAACTGTTATGCTGATTTGTTTTTTCTGACCATCTTATCCAGTTATCCCTATGTATTTACCAGATGGTAAAGGACCCATCAATCCTAAAGGACTACAATATTACAACAATCTCATAAATGAACTGATAAGCCACGGTTTGTGAAGACTATATTTTTCATTTAGAGTTTGATTAACACTTAAACGATGCTCTAATATATTTCATCTTCTAGGAATCCAAGCACATGTTACATTACATCATTGGGATCTACCACAATCACTTGAGGATGAGTATGGAGGATGGGTTAGCAAAAAAATTGTGTATGGTTCCCAAACTTTCCCATTCTGTTTGAGTTACCTATTGTCATAGATTGAAATAAATAACAAATACACAAAATTAAGAAATGGTTTTTATTGTGGTTAACATATCTATATATGTATCCTTGCAGGAAAGATTTCACATCATATGCTGATGTGTGCTTCAAAGAATTTGGCGATCGAGTTAAGTACTGGACCACGGTGAATGAGGTCAATATTTATGCAATGTTTGGCTATGATATTGGAATGGCACCGCCTCAGAGGTGTTCTCCTTCCTTTGTAGCTAACTGCTCAAGGGGAAATTCTTCAACTGAGCCATATTTGGCAGCTCATCATATGTTGTTAGCACATGCTTCTGCTGCAAGATTGTACAGGAAGAAATATAAGGTAAATTTATGTAGAACATATGCACCACTTTACTTACAATCTAATTTCTGTTTTCATTTTGAATATAGTTCTTAATCATAATTTGATTAATATGTTCATGAAACAATCTTTCTAAAGTGCAGGGTATGCAACATGGATTTATTGGGTTTAATCTTCTTACTTTTGGTTTTGTTCCACTGACAAATACTAGTGAAGATATAATTGCCACTCAAAGGGCCCAAGACTTCATGATGGGGTGGTAAGTAATGATTTGATCATGTTGTCTTTCTTTTCAATGTTTGTAGACATTTCTAAACTTTGCATTATGGGTGGTAAGTCCAAGATTTATTTTGTAATACATTAAATCTCAAAACTTTCAATTATACATTAATATCTACAAGTATGTTTTGTTTATCATTTGTTATTGCAGGGTTTTAAATCCTTTAACATTTGGTGATTATCCTGATATAATGAAAAAGAATGTTGGACCAAGACTTCCTTCCTTCACAGTAAAGGAATCAAATCTGATCAAGAGCTCAATTGACTTCTTAGGAGTAAACTTTTACAACTTAATATATGTTCAGAACAATCCTGCAAGCCTGAACATGGAGGATAGAGATTACATGCAAGATATGGCAGTGGAAATTACAAGTATGTTTATAATAGCTAAAGACTTGTTTAATCTATAATGTCATCCACTTTACTCCATTTTGAAGAAACTGAAACAACTAATTAATGCTCTTTCTTGTTGCAGTCATGATAGAAAATGATACATCAATAGATGAGGTAACATAGTGCAGTAACAAGGAATTGTTCCTCTTGTAATTTATTTATGTATTTGGTTTAACCAATTGATCATCTGGTTGGTTTAGTTTCCGATTACAACCAAGAATTTTCAAGAGGTGCTATATTACTTGAAGAATGATTATGGAAATTTTCCAATTTACATACATGAAAATGGTATGACAATTTTCCAACATATGGCATGATCATTCCATGTTTCCTTTCAAAATCTTCACAATATTTATTTATTAGTGTGATAAGCCTTCGAGTTCATTTTGCAGGCTACAGAACACTTCGCAATTCATCCTTGAATGATTGGTCTAGGGTGAAGTACTTGCATAAATACATTGGGACCATAGCTAATGAAATAAGGTACTTCTACTTTGATTGTCAAATATAATGTATAAGGTACATCTTCGTTGCTAATTATTCCTTTATTTTTGACACATCGAAAGGAGTGGATCGAATATAAAAGGTTATTTTGTATGGTCCTTCATGGATGTGCTTGAAATACTGAGTGGATATGAAGAAAGTTTTGGCCTATACTACATAGATATGAAAGATCCAAACTTGAAGAGACAACCTAAGCTCTCTGCAAAATGGTACTCAAATTTTCTGAAAAGGAAGCCTATGGACTCAAAGATTACTATGGAAATTGAGAATGATGCACCATGTAAATCATTGCTATAA
Protein sequence

>MS.gene95623.t1

MSMALILKNFSLTELLTAAAVLVVILFPSAYSLNKDDFPSDFVFGVSTSAYQFEGAANEDGRKPSIWDTFAHGGNANMYKGDGDIACDGYHKYKEDVRLMANMGLDAYRFSISWSRLIPDGKGPINPKGLQYYNNLINELISHGIQAHVTLHHWDLPQSLEDEYGGWVSKKIVKDFTSYADVCFKEFGDRVKYWTTVNEVNIYAMFGYDIGMAPPQRCSPSFVANCSRGNSSTEPYLAAHHMLLAHASAARLYRKKYKGMQHGFIGFNLLTFGFVPLTNTSEDIIATQRAQDFMMGWVLNPLTFGDYPDIMKKNVGPRLPSFTVKESNLIKSSIDFLGVNFYNLIYVQNNPASLNMEDRDYMQDMAVEITIMIENDTSIDEFPITTKNFQEVLYYLKNDYGNFPIYIHENGYRTLRNSSLNDWSRVKYLHKYIGTIANEIRSGSNIKGYFVWSFMDVLEILSGYEESFGLYYIDMKDPNLKRQPKLSAKWYSNFLKRKPMDSKITMEIENDAPCKSLL