Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95762.t1 | XP_013455584.1 | 96.8 | 154 | 5 | 0 | 1 | 154 | 1 | 154 | 2.10E-78 | 301.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95762.t1 | O05517 | 28.8 | 125 | 88 | 1 | 17 | 141 | 15 | 138 | 3.3e-08 | 59.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95762.t1 | G7ZX36 | 96.8 | 154 | 5 | 0 | 1 | 154 | 1 | 154 | 1.5e-78 | 301.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene95762.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene95762 | 0.803811 | 2.92E-49 | -1.69E-46 |
| MS.gene049873 | MS.gene95762 | 0.803643 | 3.16E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene95762 | 0.813044 | 3.12E-51 | -1.69E-46 |
| MS.gene051574 | MS.gene95762 | 0.80254 | 5.35E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene95762 | 0.806352 | 8.58E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene95762 | 0.800103 | 1.69E-48 | -1.69E-46 |
| MS.gene056050 | MS.gene95762 | 0.820666 | 6.08E-53 | -1.69E-46 |
| MS.gene056366 | MS.gene95762 | 0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene95762 | 0.80566 | 1.20E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95762.t1 | MTR_4g046047 | 96.753 | 154 | 5 | 0 | 1 | 154 | 1 | 154 | 2.07e-110 | 310 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95762.t1 | AT1G03650 | 68.421 | 152 | 45 | 1 | 6 | 154 | 6 | 157 | 1.39e-73 | 217 |
Find 28 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGTTATGTCATGTATTCT+TGG | 0.193464 | 4.1:-53811156 | MS.gene95762:CDS |
| AGTGGAAGAGATAGTGAAAT+TGG | 0.247431 | 4.1:-53811284 | MS.gene95762:CDS |
| CACGTTGATTCCCAACTTGC+AGG | 0.284196 | 4.1:-53811175 | MS.gene95762:CDS |
| CATGTTGACTGTTTGGTTGA+AGG | 0.355783 | 4.1:-53810350 | MS.gene95762:CDS |
| GAAGCGAGTGATTCGTGTTT+GGG | 0.367842 | 4.1:+53811247 | None:intergenic |
| GGAAGCGAGTGATTCGTGTT+TGG | 0.373847 | 4.1:+53811246 | None:intergenic |
| TGACTTTCATGTTGACTGTT+TGG | 0.392595 | 4.1:-53810357 | MS.gene95762:CDS |
| CATGACATAACCTGCAAGTT+GGG | 0.425799 | 4.1:+53811165 | None:intergenic |
| TGGACTTCGATTCAAATTGA+AGG | 0.474763 | 4.1:-53810289 | None:intergenic |
| GCAGAACAAGAAAAGTTTCA+CGG | 0.515102 | 4.1:-53810436 | MS.gene95762:CDS |
| AAATGCTTATAGAATGTACT+TGG | 0.517363 | 4.1:-53810309 | MS.gene95762:CDS |
| ACATGACATAACCTGCAAGT+TGG | 0.525642 | 4.1:+53811164 | None:intergenic |
| CAATGGAGGAGGCAAGGACA+CGG | 0.532439 | 4.1:-53810491 | MS.gene95762:CDS |
| GTAGTTGGAACCTGCGAGCT+TGG | 0.534236 | 4.1:+53811108 | None:intergenic |
| GCTTCTATCACCAAGCTCGC+AGG | 0.549548 | 4.1:-53811118 | MS.gene95762:intron |
| AAGGAGCAATGGAGGAGGCA+AGG | 0.558770 | 4.1:-53810497 | MS.gene95762:CDS |
| TGCTTCACGTCGATCCGTTG+AGG | 0.562820 | 4.1:-53810409 | MS.gene95762:CDS |
| ATAGAAGCATACAATGAAGA+AGG | 0.562971 | 4.1:+53811133 | None:intergenic |
| CAGAAACAGTGGTAAAGTAG+TGG | 0.572015 | 4.1:-53811302 | MS.gene95762:CDS |
| CTTCTGTAGTGAAGGAGCAA+TGG | 0.596774 | 4.1:-53810508 | MS.gene95762:intron |
| CTGTAGTGAAGGAGCAATGG+AGG | 0.602271 | 4.1:-53810505 | MS.gene95762:intron |
| TAGTGAAGGAGCAATGGAGG+AGG | 0.603189 | 4.1:-53810502 | MS.gene95762:CDS |
| CGCACGAGTGATGGCAACTG+TGG | 0.609912 | 4.1:-53811341 | None:intergenic |
| CGGAGAGGTTCTACTGAAAG+CGG | 0.611656 | 4.1:-53810471 | MS.gene95762:CDS |
| TTCAGCGCAGGTGTCCTCAA+CGG | 0.620997 | 4.1:+53810395 | None:intergenic |
| TTCTTGTACAGATTCAGCGC+AGG | 0.621123 | 4.1:+53810383 | None:intergenic |
| TTAGAACTTGACAGAAACAG+TGG | 0.638839 | 4.1:-53811313 | MS.gene95762:CDS |
| GAGGAGGCAAGGACACGGAG+AGG | 0.683029 | 4.1:-53810486 | MS.gene95762:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAACTATCTTTAATATAA+GGG | - | chr4.1:53810990-53811009 | MS.gene95762:intron | 10.0% |
| !! | CTAAACTATTAAAATATAAT+AGG | + | chr4.1:53810785-53810804 | None:intergenic | 10.0% |
| !! | GATTAACTATCTTTAATATA+AGG | - | chr4.1:53810989-53811008 | MS.gene95762:intron | 15.0% |
| !!! | ATGAAATGCTTTTAATTTGT+TGG | - | chr4.1:53811052-53811071 | MS.gene95762:intron | 20.0% |
| !!! | ATTAAAAGCATTTCATTAGT+TGG | + | chr4.1:53811048-53811067 | None:intergenic | 20.0% |
| !!! | GAGTAACTAAATTTTCAAAA+TGG | + | chr4.1:53810621-53810640 | None:intergenic | 20.0% |
| !!! | TTAAAAGCATTTCATTAGTT+GGG | + | chr4.1:53811047-53811066 | None:intergenic | 20.0% |
| ! | AAATGCTTATAGAATGTACT+TGG | - | chr4.1:53811314-53811333 | MS.gene95762:CDS | 25.0% |
| ! | ACAATATGGGTATGTAATAT+GGG | - | chr4.1:53811081-53811100 | MS.gene95762:intron | 25.0% |
| !! | AAAATTGTCTAAGTGTGTTT+TGG | - | chr4.1:53810580-53810599 | MS.gene95762:intron | 25.0% |
| !!! | CAATTTTTCAAACAGCTTAT+AGG | + | chr4.1:53810567-53810586 | None:intergenic | 25.0% |
| !!! | TTTGAGTTCATTTTCAAAGA+AGG | + | chr4.1:53810401-53810420 | None:intergenic | 25.0% |
| CACAATATGGGTATGTAATA+TGG | - | chr4.1:53811080-53811099 | MS.gene95762:intron | 30.0% | |
| TAATACATCAACTGAGTTGT+TGG | - | chr4.1:53810746-53810765 | MS.gene95762:intron | 30.0% | |
| ! | AACTCAGTTGATGTATTAAG+GGG | + | chr4.1:53810744-53810763 | None:intergenic | 30.0% |
| ! | ACAACTCAGTTGATGTATTA+AGG | + | chr4.1:53810746-53810765 | None:intergenic | 30.0% |
| ! | AGCATAAAAAAGAGTGTAGT+TGG | + | chr4.1:53810533-53810552 | None:intergenic | 30.0% |
| ! | ATAGAAGCATACAATGAAGA+AGG | + | chr4.1:53810493-53810512 | None:intergenic | 30.0% |
| ! | CAACTCAGTTGATGTATTAA+GGG | + | chr4.1:53810745-53810764 | None:intergenic | 30.0% |
| ! | GTATGTAATATGGGATGTTT+TGG | - | chr4.1:53811090-53811109 | MS.gene95762:intron | 30.0% |
| ! | TAACTTTTGTAAGTGCATCT+CGG | - | chr4.1:53810648-53810667 | MS.gene95762:intron | 30.0% |
| ! | TTGTTGGTTACTCACAATAT+GGG | - | chr4.1:53811068-53811087 | MS.gene95762:intron | 30.0% |
| ! | TTTGTTGGTTACTCACAATA+TGG | - | chr4.1:53811067-53811086 | MS.gene95762:intron | 30.0% |
| AGTGGAAGAGATAGTGAAAT+TGG | - | chr4.1:53810339-53810358 | MS.gene95762:CDS | 35.0% | |
| CAATATCGACATTGTCAGTT+AGG | - | chr4.1:53810962-53810981 | MS.gene95762:intron | 35.0% | |
| CAATGTCGATATTGTTAGGT+TGG | + | chr4.1:53810956-53810975 | None:intergenic | 35.0% | |
| CAGGTTATGTCATGTATTCT+TGG | - | chr4.1:53810467-53810486 | MS.gene95762:CDS | 35.0% | |
| CTGACAATGTCGATATTGTT+AGG | + | chr4.1:53810960-53810979 | None:intergenic | 35.0% | |
| GAACTCAAAAAGAAGAACAG+TGG | - | chr4.1:53810412-53810431 | MS.gene95762:CDS | 35.0% | |
| GCAGAACAAGAAAAGTTTCA+CGG | - | chr4.1:53811187-53811206 | MS.gene95762:CDS | 35.0% | |
| TTAGAACTTGACAGAAACAG+TGG | - | chr4.1:53810310-53810329 | MS.gene95762:CDS | 35.0% | |
| TTGTGTGTGTGAATTTCATG+TGG | + | chr4.1:53810899-53810918 | None:intergenic | 35.0% | |
| ! | ACTCAGTTGATGTATTAAGG+GGG | + | chr4.1:53810743-53810762 | None:intergenic | 35.0% |
| ! | TGACTTTCATGTTGACTGTT+TGG | - | chr4.1:53811266-53811285 | MS.gene95762:CDS | 35.0% |
| !! | ACTCAACTGATTTGAGCTAA+AGG | + | chr4.1:53810717-53810736 | None:intergenic | 35.0% |
| !! | CTCAACTGATTTGAGCTAAA+GGG | + | chr4.1:53810716-53810735 | None:intergenic | 35.0% |
| !!! | TTTTGGTTCTTCTGTAGTGA+AGG | - | chr4.1:53811107-53811126 | MS.gene95762:intron | 35.0% |
| AATTCACACACACAACTGCA+AGG | - | chr4.1:53810904-53810923 | MS.gene95762:intron | 40.0% | |
| ACATGACATAACCTGCAAGT+TGG | + | chr4.1:53810462-53810481 | None:intergenic | 40.0% | |
| CAGAAACAGTGGTAAAGTAG+TGG | - | chr4.1:53810321-53810340 | MS.gene95762:CDS | 40.0% | |
| CATGACATAACCTGCAAGTT+GGG | + | chr4.1:53810461-53810480 | None:intergenic | 40.0% | |
| GCATTTCATTAGTTGGGAGT+TGG | + | chr4.1:53811041-53811060 | None:intergenic | 40.0% | |
| ! | CATGTTGACTGTTTGGTTGA+AGG | - | chr4.1:53811273-53811292 | MS.gene95762:CDS | 40.0% |
| ! | GGTGATTGTCACTTCATCTT+CGG | + | chr4.1:53810878-53810897 | None:intergenic | 40.0% |
| ! | TTTTGTAAGTGCATCTCGGT+TGG | - | chr4.1:53810652-53810671 | MS.gene95762:intron | 40.0% |
| ACACACAACTGCAAGGTCTT+AGG | - | chr4.1:53810911-53810930 | MS.gene95762:intron | 45.0% | |
| CTTCTGTAGTGAAGGAGCAA+TGG | - | chr4.1:53811115-53811134 | MS.gene95762:intron | 45.0% | |
| GAAGCGAGTGATTCGTGTTT+GGG | + | chr4.1:53810379-53810398 | None:intergenic | 45.0% | |
| TTCTTGTACAGATTCAGCGC+AGG | + | chr4.1:53811243-53811262 | None:intergenic | 45.0% | |
| !! | AAGCTTGGGCATTGGTATAC+TGG | - | chr4.1:53810676-53810695 | MS.gene95762:intron | 45.0% |
| !! | AGCTTGGGCATTGGTATACT+GGG | - | chr4.1:53810677-53810696 | MS.gene95762:intron | 45.0% |
| CGGAGAGGTTCTACTGAAAG+CGG | - | chr4.1:53811152-53811171 | MS.gene95762:CDS | 50.0% | |
| CTGTAGTGAAGGAGCAATGG+AGG | - | chr4.1:53811118-53811137 | MS.gene95762:intron | 50.0% | |
| GGAAGCGAGTGATTCGTGTT+TGG | + | chr4.1:53810380-53810399 | None:intergenic | 50.0% | |
| TAGTGAAGGAGCAATGGAGG+AGG | - | chr4.1:53811121-53811140 | MS.gene95762:CDS | 50.0% | |
| !! | CACGTTGATTCCCAACTTGC+AGG | - | chr4.1:53810448-53810467 | MS.gene95762:CDS | 50.0% |
| !! | GCATCTCGGTTGGTAAGCTT+GGG | - | chr4.1:53810662-53810681 | MS.gene95762:intron | 50.0% |
| !! | GGCATTGGTATACTGGGTTC+GGG | - | chr4.1:53810683-53810702 | MS.gene95762:intron | 50.0% |
| !! | GGGCATTGGTATACTGGGTT+CGG | - | chr4.1:53810682-53810701 | MS.gene95762:intron | 50.0% |
| !! | TGCATCTCGGTTGGTAAGCT+TGG | - | chr4.1:53810661-53810680 | MS.gene95762:intron | 50.0% |
| AAGGAGCAATGGAGGAGGCA+AGG | - | chr4.1:53811126-53811145 | MS.gene95762:CDS | 55.0% | |
| CAATGGAGGAGGCAAGGACA+CGG | - | chr4.1:53811132-53811151 | MS.gene95762:CDS | 55.0% | |
| GCTTCTATCACCAAGCTCGC+AGG | - | chr4.1:53810505-53810524 | MS.gene95762:intron | 55.0% | |
| GTAGTTGGAACCTGCGAGCT+TGG | + | chr4.1:53810518-53810537 | None:intergenic | 55.0% | |
| TGCTTCACGTCGATCCGTTG+AGG | - | chr4.1:53811214-53811233 | MS.gene95762:CDS | 55.0% | |
| TTCAGCGCAGGTGTCCTCAA+CGG | + | chr4.1:53811231-53811250 | None:intergenic | 55.0% | |
| !! | CGGTTGGTAAGCTTGGGCAT+TGG | - | chr4.1:53810668-53810687 | MS.gene95762:intron | 55.0% |
| GAGGAGGCAAGGACACGGAG+AGG | - | chr4.1:53811137-53811156 | MS.gene95762:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 53810292 | 53811353 | 53810292 | ID=MS.gene95762 |
| chr4.1 | mRNA | 53810292 | 53811353 | 53810292 | ID=MS.gene95762.t1;Parent=MS.gene95762 |
| chr4.1 | exon | 53811119 | 53811353 | 53811119 | ID=MS.gene95762.t1.exon1;Parent=MS.gene95762.t1 |
| chr4.1 | CDS | 53811119 | 53811353 | 53811119 | ID=cds.MS.gene95762.t1;Parent=MS.gene95762.t1 |
| chr4.1 | exon | 53810292 | 53810521 | 53810292 | ID=MS.gene95762.t1.exon2;Parent=MS.gene95762.t1 |
| chr4.1 | CDS | 53810292 | 53810521 | 53810292 | ID=cds.MS.gene95762.t1;Parent=MS.gene95762.t1 |
| Gene Sequence |
| Protein sequence |
