AlfalfaGEDB | MS.gene98700

M. sativa cultivar XinJiangDaYe / MS.gene98700

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008168 GO:0016740 GO:0016741
PFAM: PF08241.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98700.t1	RHN46565.1	93.9	212	12	1	1	211	10	221	8.30E-102	379.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98700.t1	G7KU52	93.9	212	12	1	1	211	10	221	6.0e-102	379.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050807	MS.gene98700	0.8257	4.06E-54	-1.69E-46
MS.gene050808	MS.gene98700	0.819843	9.39E-53	-1.69E-46
MS.gene052239	MS.gene98700	-0.810939	8.98E-51	-1.69E-46
MS.gene052673	MS.gene98700	-0.800563	1.36E-48	-1.69E-46
MS.gene052858	MS.gene98700	0.804879	1.75E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98700.t1	MTR_7g070530	93.868	212	12	1	1	211	10	221	4.38e-137	394
MS.gene98700.t1	MTR_4g013290	53.456	217	79	4	1	200	1	212	2.49e-62	202
MS.gene98700.t1	MTR_3g031200	46.154	195	100	3	6	200	2	191	3.39e-48	164
MS.gene98700.t1	MTR_1g099090	41.975	162	73	5	40	200	10	151	7.14e-29	106
MS.gene98700.t1	MTR_8g100030	45.783	83	45	0	80	162	53	135	8.59e-19	79.3
MS.gene98700.t1	MTR_7g057130	74.468	47	12	0	103	149	27	73	1.14e-16	74.3
MS.gene98700.t1	MTR_8g089585	34.677	124	77	2	81	202	87	208	4.32e-15	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98700.t1	AT5G01710	53.302	212	84	3	1	200	1	209	2.10e-67	215
MS.gene98700.t1	AT1G24480	37.097	124	74	2	80	201	74	195	3.25e-18	80.5
MS.gene98700.t1	AT4G24805	38.211	123	72	2	81	201	90	210	9.19e-16	73.9
MS.gene98700.t1	AT2G16030	33.333	117	73	2	70	186	72	183	4.07e-13	66.2

Find 52 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAACTATACGTATCTTTA+CGG	0.252227	7.1:-31387539	None:intergenic
GGTGAAACCGCTTGTGAAAT+CGG	0.305003	7.1:+31387362	MS.gene98700:CDS
ATAGCGAGACTGATTGTTTC+CGG	0.310156	7.1:-31387120	None:intergenic
CTTTGTGTTGAAACCGTTAC+CGG	0.321088	7.1:+31387276	MS.gene98700:CDS
TTCTCCGGTGAAGGCTCGTT+CGG	0.323955	7.1:+31387432	MS.gene98700:CDS
TTTCTGCTTCTTCTCGTTGC+CGG	0.347913	7.1:+31387101	MS.gene98700:CDS
CTCTCCGGTGAATCATCTTC+CGG	0.388754	7.1:+31387159	MS.gene98700:CDS
GTAACCGTATCAGAAGATTC+TGG	0.392916	7.1:-31386999	None:intergenic
TCTCCGGTGAAGGCTCGTTC+GGG	0.404734	7.1:+31387433	MS.gene98700:CDS
GGCGAAGCCGGCGGTGTTTG+CGG	0.405581	7.1:+31387461	MS.gene98700:CDS
GTGAAACCGCTTGTGAAATC+GGG	0.426976	7.1:+31387363	MS.gene98700:CDS
CAACGAGAAGAAGCAGAAAT+CGG	0.436584	7.1:-31387097	None:intergenic
ACCGGAGAGAGGTTCATTAC+CGG	0.448505	7.1:-31387145	None:intergenic
GTTCGGGAAGTCGGCGAAGC+CGG	0.452260	7.1:+31387449	MS.gene98700:CDS
CGTCGGAGTCGCGAGGAAAT+CGG	0.465251	7.1:+31387341	MS.gene98700:CDS
AAATACGTCATCGCCGGCCG+TGG	0.479239	7.1:+31387188	MS.gene98700:CDS
CTTTCCAGAATCTTCTGATA+CGG	0.483053	7.1:+31386995	MS.gene98700:CDS
AGTGGTGAACGGATTCCGTT+TGG	0.488237	7.1:+31387387	MS.gene98700:CDS
ATTGCAAGACTCGCCGGCGA+TGG	0.501779	7.1:-31387070	None:intergenic
TGTTTGCGGCGGAGATTGCT+CGG	0.504065	7.1:+31387475	MS.gene98700:CDS
CTTCCCGAACGAGCCTTCAC+CGG	0.515338	7.1:-31387436	None:intergenic
TGAAGGCTCGTTCGGGAAGT+CGG	0.515472	7.1:+31387440	MS.gene98700:CDS
TTTCGTGACTTGATCGGCGG+AGG	0.515739	7.1:+31387228	MS.gene98700:CDS
CCACTTCCCGATTTCACAAG+CGG	0.518254	7.1:-31387369	None:intergenic
GATGAAACACGGTGGATTCG+CGG	0.524712	7.1:+31387500	MS.gene98700:CDS
AGAAGATTCTGGAAAGAGCT+AGG	0.524979	7.1:-31386988	None:intergenic
CGATGACGTATTTGCGCCAC+CGG	0.525208	7.1:-31387178	None:intergenic
ACAATCAGTCTCGCTATCTC+CGG	0.525437	7.1:+31387126	MS.gene98700:CDS
ATCTCCGCCGCAAACACCGC+CGG	0.530458	7.1:-31387468	None:intergenic
GAAGAGTAGAAAGCCACGGC+CGG	0.538717	7.1:-31387201	None:intergenic
GGCGACATTGCAAGACTCGC+CGG	0.544591	7.1:-31387076	None:intergenic
TCAAACTCACCGTCACCAAA+CGG	0.547214	7.1:-31387402	None:intergenic
CCGCTTGTGAAATCGGGAAG+TGG	0.559701	7.1:+31387369	MS.gene98700:CDS
CGATTTCCTCGCGACTCCGA+CGG	0.560272	7.1:-31387340	None:intergenic
TCCGGTAATGAACCTCTCTC+CGG	0.561229	7.1:+31387144	MS.gene98700:CDS
CTGATACGGTTACTTCTCAC+CGG	0.578963	7.1:+31387009	MS.gene98700:CDS
CGGGAAGTCGGCGAAGCCGG+CGG	0.589704	7.1:+31387452	MS.gene98700:CDS
GCTTACGTCATCTCCATCGC+CGG	0.590644	7.1:+31387057	MS.gene98700:CDS
GAACGGATTCCGTTTGGTGA+CGG	0.594862	7.1:+31387393	MS.gene98700:CDS
TGGCGCAAATACGTCATCGC+CGG	0.595835	7.1:+31387182	MS.gene98700:CDS
GAAGCCGGCGGTGTTTGCGG+CGG	0.603669	7.1:+31387464	MS.gene98700:CDS
TCCGGTGAATCATCTTCCGG+TGG	0.610828	7.1:+31387162	MS.gene98700:CDS
GCCACCGGAAGATGATTCAC+CGG	0.619775	7.1:-31387163	None:intergenic
TGAAATCGGGAAGTGGTGAA+CGG	0.620413	7.1:+31387376	MS.gene98700:CDS
AACAGAAGAGTAGAAAGCCA+CGG	0.629536	7.1:-31387205	None:intergenic
GAAGATGATTCACCGGAGAG+AGG	0.633378	7.1:-31387156	None:intergenic
AAAACGCCGTCGGAGTCGCG+AGG	0.651986	7.1:+31387334	MS.gene98700:CDS
ATTGCTCGGACGATGAAACA+CGG	0.696867	7.1:+31387489	MS.gene98700:CDS
AACAGCAATGACGAAAACGC+CGG	0.715270	7.1:-31387028	None:intergenic
CGGCGGAGGTTACTTGACGA+CGG	0.733582	7.1:+31387242	MS.gene98700:CDS
GCTCGGACGATGAAACACGG+TGG	0.794839	7.1:+31387492	MS.gene98700:CDS
GAAAGAGCTAGGTTTCGCTG+CGG	0.796736	7.1:-31386977	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGAAACTATACGTATCTTTA+CGG	-	chr7.1:31387542-31387561	None:intergenic	25.0%
!!	GAGTTTGATTTTGTATTCTC+CGG	+	chr7.1:31387417-31387436	MS.gene98700:CDS	30.0%
	CTTTCCAGAATCTTCTGATA+CGG	+	chr7.1:31386995-31387014	MS.gene98700:CDS	35.0%
!!	TCTGTTTTTCGTGACTTGAT+CGG	+	chr7.1:31387222-31387241	MS.gene98700:CDS	35.0%
!!!	GTTTTTGCTCTGAGAGAAAT+CGG	+	chr7.1:31387306-31387325	MS.gene98700:CDS	35.0%
	AAAAACATCACGTCCGGTAA+CGG	-	chr7.1:31387292-31387311	None:intergenic	40.0%
	AACAGAAGAGTAGAAAGCCA+CGG	-	chr7.1:31387208-31387227	None:intergenic	40.0%
	ATAGCGAGACTGATTGTTTC+CGG	-	chr7.1:31387123-31387142	None:intergenic	40.0%
	CAACGAGAAGAAGCAGAAAT+CGG	-	chr7.1:31387100-31387119	None:intergenic	40.0%
	CTTTGTGTTGAAACCGTTAC+CGG	+	chr7.1:31387276-31387295	MS.gene98700:CDS	40.0%
	GTAACCGTATCAGAAGATTC+TGG	-	chr7.1:31387002-31387021	None:intergenic	40.0%
!	AGAAGATTCTGGAAAGAGCT+AGG	-	chr7.1:31386991-31387010	None:intergenic	40.0%
!	GATTTTGTATTCTCCGGTGA+AGG	+	chr7.1:31387423-31387442	MS.gene98700:CDS	40.0%
	AACAGCAATGACGAAAACGC+CGG	-	chr7.1:31387031-31387050	None:intergenic	45.0%
	ACAATCAGTCTCGCTATCTC+CGG	+	chr7.1:31387126-31387145	MS.gene98700:CDS	45.0%
	ATCGGAGTAAAAAACGCCGT+CGG	+	chr7.1:31387324-31387343	MS.gene98700:CDS	45.0%
	ATTGCTCGGACGATGAAACA+CGG	+	chr7.1:31387489-31387508	MS.gene98700:CDS	45.0%
	CAGAGCAAAAACATCACGTC+CGG	-	chr7.1:31387298-31387317	None:intergenic	45.0%
	CTGATACGGTTACTTCTCAC+CGG	+	chr7.1:31387009-31387028	MS.gene98700:CDS	45.0%
	GGTGAAACCGCTTGTGAAAT+CGG	+	chr7.1:31387362-31387381	MS.gene98700:CDS	45.0%
	GTGAAACCGCTTGTGAAATC+GGG	+	chr7.1:31387363-31387382	MS.gene98700:CDS	45.0%
	TCAAACTCACCGTCACCAAA+CGG	-	chr7.1:31387405-31387424	None:intergenic	45.0%
	TGAAATCGGGAAGTGGTGAA+CGG	+	chr7.1:31387376-31387395	MS.gene98700:CDS	45.0%
	TTTCTGCTTCTTCTCGTTGC+CGG	+	chr7.1:31387101-31387120	MS.gene98700:CDS	45.0%
!	GTTTTTCGTGACTTGATCGG+CGG	+	chr7.1:31387225-31387244	MS.gene98700:CDS	45.0%
	ACCGGAGAGAGGTTCATTAC+CGG	-	chr7.1:31387148-31387167	None:intergenic	50.0%
	CCACTTCCCGATTTCACAAG+CGG	-	chr7.1:31387372-31387391	None:intergenic	50.0%
	CTCTCCGGTGAATCATCTTC+CGG	+	chr7.1:31387159-31387178	MS.gene98700:CDS	50.0%
	GAAAGAGCTAGGTTTCGCTG+CGG	-	chr7.1:31386980-31386999	None:intergenic	50.0%
	GAAGATGATTCACCGGAGAG+AGG	-	chr7.1:31387159-31387178	None:intergenic	50.0%
	TCCGGTAATGAACCTCTCTC+CGG	+	chr7.1:31387144-31387163	MS.gene98700:CDS	50.0%
!	AGTGGTGAACGGATTCCGTT+TGG	+	chr7.1:31387387-31387406	MS.gene98700:CDS	50.0%
!	GAACGGATTCCGTTTGGTGA+CGG	+	chr7.1:31387393-31387412	MS.gene98700:CDS	50.0%
!	GATGAAACACGGTGGATTCG+CGG	+	chr7.1:31387500-31387519	MS.gene98700:CDS	50.0%
	CCGCTTGTGAAATCGGGAAG+TGG	+	chr7.1:31387369-31387388	MS.gene98700:CDS	55.0%
	CGATGACGTATTTGCGCCAC+CGG	-	chr7.1:31387181-31387200	None:intergenic	55.0%
	GAAGAGTAGAAAGCCACGGC+CGG	-	chr7.1:31387204-31387223	None:intergenic	55.0%
	GCCACCGGAAGATGATTCAC+CGG	-	chr7.1:31387166-31387185	None:intergenic	55.0%
	GCTTACGTCATCTCCATCGC+CGG	+	chr7.1:31387057-31387076	MS.gene98700:CDS	55.0%
	TCCGGTGAATCATCTTCCGG+TGG	+	chr7.1:31387162-31387181	MS.gene98700:CDS	55.0%
	TGAAGGCTCGTTCGGGAAGT+CGG	+	chr7.1:31387440-31387459	MS.gene98700:CDS	55.0%
	TGGCGCAAATACGTCATCGC+CGG	+	chr7.1:31387182-31387201	MS.gene98700:CDS	55.0%
	TGTTTGCGGCGGAGATTGCT+CGG	+	chr7.1:31387475-31387494	MS.gene98700:CDS	55.0%
	TTCTCCGGTGAAGGCTCGTT+CGG	+	chr7.1:31387432-31387451	MS.gene98700:CDS	55.0%
	TTTCGTGACTTGATCGGCGG+AGG	+	chr7.1:31387228-31387247	MS.gene98700:CDS	55.0%
	AAATACGTCATCGCCGGCCG+TGG	+	chr7.1:31387188-31387207	MS.gene98700:CDS	60.0%
	ATTGCAAGACTCGCCGGCGA+TGG	-	chr7.1:31387073-31387092	None:intergenic	60.0%
	CGATTTCCTCGCGACTCCGA+CGG	-	chr7.1:31387343-31387362	None:intergenic	60.0%
	CGGCGGAGGTTACTTGACGA+CGG	+	chr7.1:31387242-31387261	MS.gene98700:CDS	60.0%
	CGTCGGAGTCGCGAGGAAAT+CGG	+	chr7.1:31387341-31387360	MS.gene98700:CDS	60.0%
	CTTCCCGAACGAGCCTTCAC+CGG	-	chr7.1:31387439-31387458	None:intergenic	60.0%
	GCTCGGACGATGAAACACGG+TGG	+	chr7.1:31387492-31387511	MS.gene98700:CDS	60.0%
	GGCGACATTGCAAGACTCGC+CGG	-	chr7.1:31387079-31387098	None:intergenic	60.0%
	TCTCCGGTGAAGGCTCGTTC+GGG	+	chr7.1:31387433-31387452	MS.gene98700:CDS	60.0%
	AAAACGCCGTCGGAGTCGCG+AGG	+	chr7.1:31387334-31387353	MS.gene98700:CDS	65.0%
	ATCTCCGCCGCAAACACCGC+CGG	-	chr7.1:31387471-31387490	None:intergenic	65.0%
	GTTCGGGAAGTCGGCGAAGC+CGG	+	chr7.1:31387449-31387468	MS.gene98700:CDS	65.0%
	GAAGCCGGCGGTGTTTGCGG+CGG	+	chr7.1:31387464-31387483	MS.gene98700:CDS	70.0%
	GGCGAAGCCGGCGGTGTTTG+CGG	+	chr7.1:31387461-31387480	MS.gene98700:CDS	70.0%
	CGGGAAGTCGGCGAAGCCGG+CGG	+	chr7.1:31387452-31387471	MS.gene98700:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	31386967	31387602	31386967	ID=MS.gene98700
chr7.1	mRNA	31386967	31387602	31386967	ID=MS.gene98700.t1;Parent=MS.gene98700
chr7.1	exon	31386967	31387602	31386967	ID=MS.gene98700.t1.exon1;Parent=MS.gene98700.t1
chr7.1	CDS	31386967	31387602	31386967	ID=cds.MS.gene98700.t1;Parent=MS.gene98700.t1

Gene Sequence

>MS.gene98700

ATGGAACACGCCGCAGCGAAACCTAGCTCTTTCCAGAATCTTCTGATACGGTTACTTCTCACCGGCGTTTTCGTCATTGCTGTTCGATTTGCTTACGTCATCTCCATCGCCGGCGAGTCTTGCAATGTCGCCGATTTCTGCTTCTTCTCGTTGCCGGAAACAATCAGTCTCGCTATCTCCGGTAATGAACCTCTCTCCGGTGAATCATCTTCCGGTGGCGCAAATACGTCATCGCCGGCCGTGGCTTTCTACTCTTCTGTTTTTCGTGACTTGATCGGCGGAGGTTACTTGACGACGGAGTCGAAGTCGCTTTGTGTTGAAACCGTTACCGGACGTGATGTTTTTGCTCTGAGAGAAATCGGAGTAAAAAACGCCGTCGGAGTCGCGAGGAAATCGGTGAAACCGCTTGTGAAATCGGGAAGTGGTGAACGGATTCCGTTTGGTGACGGTGAGTTTGATTTTGTATTCTCCGGTGAAGGCTCGTTCGGGAAGTCGGCGAAGCCGGCGGTGTTTGCGGCGGAGATTGCTCGGACGATGAAACACGGTGGATTCGCGGTTTTTCATTTCAGAAACCGTAAAGATACGTATAGTTTCAATTCAATTCATTTCTTGATTTGTTTCATTGTTTCAAAGTAG

Protein sequence

>MS.gene98700.t1

MEHAAAKPSSFQNLLIRLLLTGVFVIAVRFAYVISIAGESCNVADFCFFSLPETISLAISGNEPLSGESSSGGANTSSPAVAFYSSVFRDLIGGGYLTTESKSLCVETVTGRDVFALREIGVKNAVGVARKSVKPLVKSGSGERIPFGDGEFDFVFSGEGSFGKSAKPAVFAAEIARTMKHGGFAVFHFRNRKDTYSFNSIHFLICFIVSK