Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99415.t1 | XP_003617057.2 | 96.5 | 254 | 9 | 0 | 1 | 254 | 1 | 254 | 1.70E-88 | 335.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99415.t1 | D9UC01 | 34.1 | 88 | 57 | 1 | 14 | 100 | 16 | 103 | 1.0e-09 | 65.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99415.t1 | G7KHG4 | 96.5 | 254 | 9 | 0 | 1 | 254 | 1 | 254 | 1.2e-88 | 335.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049698 | MS.gene99415 | 0.819778 | 9.71E-53 | -1.69E-46 |
| MS.gene054118 | MS.gene99415 | 0.817798 | 2.74E-52 | -1.69E-46 |
| MS.gene058909 | MS.gene99415 | 0.810128 | 1.34E-50 | -1.69E-46 |
| MS.gene058912 | MS.gene99415 | 0.806685 | 7.30E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99415.t1 | MTR_5g087450 | 95.669 | 254 | 11 | 0 | 1 | 254 | 1 | 254 | 9.06e-157 | 436 |
| MS.gene99415.t1 | MTR_5g086020 | 91.339 | 127 | 11 | 0 | 62 | 188 | 1 | 127 | 1.30e-69 | 210 |
| MS.gene99415.t1 | MTR_5g087470 | 44.172 | 163 | 79 | 4 | 11 | 169 | 10 | 164 | 4.90e-35 | 126 |
| MS.gene99415.t1 | MTR_5g087480 | 37.143 | 175 | 97 | 4 | 9 | 178 | 8 | 174 | 1.13e-27 | 108 |
| MS.gene99415.t1 | MTR_5g087480 | 31.741 | 293 | 136 | 10 | 9 | 243 | 8 | 294 | 2.83e-27 | 107 |
| MS.gene99415.t1 | MTR_7g086130 | 40.860 | 93 | 55 | 0 | 11 | 103 | 8 | 100 | 8.17e-20 | 84.7 |
| MS.gene99415.t1 | MTR_6g478190 | 40.659 | 91 | 54 | 0 | 10 | 100 | 10 | 100 | 2.53e-18 | 80.1 |
| MS.gene99415.t1 | MTR_7g086130 | 38.776 | 98 | 55 | 1 | 11 | 103 | 8 | 105 | 1.38e-17 | 79.0 |
| MS.gene99415.t1 | MTR_4g107860 | 27.234 | 235 | 156 | 5 | 13 | 237 | 21 | 250 | 6.12e-16 | 75.5 |
| MS.gene99415.t1 | MTR_8g046245 | 36.667 | 90 | 56 | 1 | 12 | 100 | 22 | 111 | 6.22e-15 | 73.9 |
| MS.gene99415.t1 | MTR_1g076910 | 34.454 | 119 | 63 | 4 | 20 | 125 | 77 | 193 | 7.67e-13 | 65.9 |
| MS.gene99415.t1 | MTR_3g007660 | 30.328 | 122 | 74 | 3 | 13 | 123 | 19 | 140 | 3.96e-12 | 65.9 |
| MS.gene99415.t1 | MTR_1g076880 | 32.174 | 115 | 65 | 3 | 22 | 125 | 78 | 190 | 1.17e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99415.t1 | AT2G27140 | 38.764 | 178 | 93 | 4 | 10 | 174 | 10 | 184 | 1.81e-32 | 119 |
| MS.gene99415.t1 | AT5G20970 | 34.137 | 249 | 134 | 6 | 11 | 243 | 7 | 241 | 2.53e-27 | 105 |
| MS.gene99415.t1 | AT1G54400 | 34.400 | 125 | 77 | 2 | 13 | 136 | 12 | 132 | 3.37e-13 | 66.6 |
| MS.gene99415.t1 | AT5G04890 | 34.091 | 88 | 57 | 1 | 14 | 100 | 16 | 103 | 7.41e-13 | 67.8 |
| MS.gene99415.t1 | AT4G10250 | 37.363 | 91 | 46 | 2 | 22 | 101 | 74 | 164 | 6.77e-12 | 63.2 |
Find 38 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGATTCCCGCTTCAAATT+TGG | 0.203921 | 5.1:-68916374 | None:intergenic |
| ACTTCTTCAATTTCTTTGTT+TGG | 0.273292 | 5.1:-68916565 | None:intergenic |
| TGTTTCTGAGGAATTGTTGC+TGG | 0.337279 | 5.1:-68916490 | None:intergenic |
| AAATTGAAGAAGTGTCTAAA+AGG | 0.356274 | 5.1:+68916575 | MS.gene99415:CDS |
| GAAGAAGCTGGTTAATTTGT+TGG | 0.370154 | 5.1:+68916765 | MS.gene99415:CDS |
| ACACTTATTCTTATGCTTCC+AGG | 0.372973 | 5.1:+68915991 | MS.gene99415:CDS |
| GTTTGGGAAGCTTAACATAT+AGG | 0.376009 | 5.1:-68916393 | None:intergenic |
| AAAGTCATTGACTATGGAAA+TGG | 0.386319 | 5.1:+68916678 | MS.gene99415:CDS |
| AATTTGAACCACCCTCTGAT+TGG | 0.401334 | 5.1:+68915947 | MS.gene99415:CDS |
| AATTAATCAATTACAACTCT+TGG | 0.411666 | 5.1:-68916867 | None:intergenic |
| ATTTGAACCACCCTCTGATT+GGG | 0.445092 | 5.1:+68915948 | MS.gene99415:CDS |
| ACCCGTGTGCTAAGGGTTAG+TGG | 0.463763 | 5.1:+68916265 | MS.gene99415:CDS |
| AACCCTTAGCACACGGGTTG+AGG | 0.474881 | 5.1:-68916260 | None:intergenic |
| GTGTCTAAAAGGAAGGGAAA+AGG | 0.475085 | 5.1:+68916586 | MS.gene99415:CDS |
| TGAAGAAGTGTCTAAAAGGA+AGG | 0.482631 | 5.1:+68916579 | MS.gene99415:CDS |
| TGTGAATGCATTATGTGGTT+TGG | 0.495211 | 5.1:+68916723 | MS.gene99415:CDS |
| TAACCTCAACCCGTGTGCTA+AGG | 0.497781 | 5.1:+68916257 | MS.gene99415:CDS |
| CATTCTGACACTAGCAGCAT+TGG | 0.497948 | 5.1:+68916349 | MS.gene99415:CDS |
| CACCACTAACCCTTAGCACA+CGG | 0.507093 | 5.1:-68916267 | None:intergenic |
| ATTTAAGAAGGATCAATTGA+GGG | 0.509297 | 5.1:+68916228 | MS.gene99415:CDS |
| GGAATTGTTGCTGGTTGTTG+AGG | 0.516048 | 5.1:-68916481 | None:intergenic |
| GAATGCATTATGTGGTTTGG+TGG | 0.533238 | 5.1:+68916726 | MS.gene99415:CDS |
| GATTTAAGAAGGATCAATTG+AGG | 0.534085 | 5.1:+68916227 | MS.gene99415:CDS |
| AATACATGCAGGATTTAAGA+AGG | 0.538421 | 5.1:+68916216 | MS.gene99415:intron |
| AACCTCAACCCGTGTGCTAA+GGG | 0.546287 | 5.1:+68916258 | MS.gene99415:CDS |
| AGTTAAGAAGCAGAAGAAGC+TGG | 0.549223 | 5.1:+68916753 | MS.gene99415:CDS |
| ATTGGTGCCAAATTTGAAGC+GGG | 0.549918 | 5.1:+68916367 | MS.gene99415:CDS |
| ACCACTAACCCTTAGCACAC+GGG | 0.576619 | 5.1:-68916266 | None:intergenic |
| GAATATGTGAATGCATTATG+TGG | 0.588342 | 5.1:+68916718 | MS.gene99415:CDS |
| GAAGAAGTGTCTAAAAGGAA+GGG | 0.593904 | 5.1:+68916580 | MS.gene99415:CDS |
| CATTGGTGCCAAATTTGAAG+CGG | 0.594150 | 5.1:+68916366 | MS.gene99415:CDS |
| ACCCTCTGATTGGGATCATG+AGG | 0.602046 | 5.1:+68915957 | MS.gene99415:CDS |
| TCCTCATGATCCCAATCAGA+GGG | 0.610175 | 5.1:-68915958 | None:intergenic |
| GAAGGGAAAAGGTAAAGCTG+AGG | 0.645097 | 5.1:+68916597 | MS.gene99415:CDS |
| ATCCTCATGATCCCAATCAG+AGG | 0.659662 | 5.1:-68915959 | None:intergenic |
| ATGCTGCTAGTGTCAGAATG+AGG | 0.675243 | 5.1:-68916346 | None:intergenic |
| TCATGATCCCAATCAGAGGG+TGG | 0.690466 | 5.1:-68915955 | None:intergenic |
| CTGCTAGTGTCAGAATGAGG+TGG | 0.722333 | 5.1:-68916343 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAATTAATTCATTTTTTTC+AGG | + | chr5.1:68916832-68916851 | MS.gene99415:CDS | 15.0% |
| !!! | TTTCTTTTGATAAAACTTAA+AGG | + | chr5.1:68916118-68916137 | MS.gene99415:intron | 15.0% |
| !! | TGAATTTATAAACTATAGCT+AGG | - | chr5.1:68916097-68916116 | None:intergenic | 20.0% |
| !!! | ATTAATTCATTTTTTTCAGG+AGG | + | chr5.1:68916835-68916854 | MS.gene99415:CDS | 20.0% |
| ! | ACTTCTTCAATTTCTTTGTT+TGG | - | chr5.1:68916568-68916587 | None:intergenic | 25.0% |
| ! | ATTTAAGAAGGATCAATTGA+GGG | + | chr5.1:68916228-68916247 | MS.gene99415:CDS | 25.0% |
| ! | GACAAATATATAAGTATACG+AGG | + | chr5.1:68916142-68916161 | MS.gene99415:intron | 25.0% |
| ! | GACAAATGAATGAGAAAAAA+TGG | + | chr5.1:68916293-68916312 | MS.gene99415:CDS | 25.0% |
| ! | TATTATATGAAAAAGACACG+AGG | - | chr5.1:68916035-68916054 | None:intergenic | 25.0% |
| !! | TGAAAAAAAGTCATTGACTA+TGG | + | chr5.1:68916672-68916691 | MS.gene99415:CDS | 25.0% |
| !!! | AAATTGAAGAAGTGTCTAAA+AGG | + | chr5.1:68916575-68916594 | MS.gene99415:CDS | 25.0% |
| AAGAAAGAAATGAACTAACC+TGG | - | chr5.1:68916012-68916031 | None:intergenic | 30.0% | |
| AATACATGCAGGATTTAAGA+AGG | + | chr5.1:68916216-68916235 | MS.gene99415:intron | 30.0% | |
| GAATATGTGAATGCATTATG+TGG | + | chr5.1:68916718-68916737 | MS.gene99415:CDS | 30.0% | |
| GATTTAAGAAGGATCAATTG+AGG | + | chr5.1:68916227-68916246 | MS.gene99415:CDS | 30.0% | |
| ! | AAAGTCATTGACTATGGAAA+TGG | + | chr5.1:68916678-68916697 | MS.gene99415:CDS | 30.0% |
| ! | GTGTTGGAACAATATTTTGT+TGG | - | chr5.1:68916426-68916445 | None:intergenic | 30.0% |
| !!! | ATTTTGTTGGTTGATGAGTT+TGG | - | chr5.1:68916413-68916432 | None:intergenic | 30.0% |
| !!! | GATGTACTACTTGTTTTTTC+TGG | - | chr5.1:68916540-68916559 | None:intergenic | 30.0% |
| !!! | TGTTGTGTTGTTGATTTTGT+TGG | - | chr5.1:68916448-68916467 | None:intergenic | 30.0% |
| !!! | TTTTGTTGGTTGATGAGTTT+GGG | - | chr5.1:68916412-68916431 | None:intergenic | 30.0% |
| ACACTTATTCTTATGCTTCC+AGG | + | chr5.1:68915991-68916010 | MS.gene99415:CDS | 35.0% | |
| CTATGATCGATAATACATGC+AGG | + | chr5.1:68916205-68916224 | MS.gene99415:intron | 35.0% | |
| GTTTGGGAAGCTTAACATAT+AGG | - | chr5.1:68916396-68916415 | None:intergenic | 35.0% | |
| TGTGAATGCATTATGTGGTT+TGG | + | chr5.1:68916723-68916742 | MS.gene99415:CDS | 35.0% | |
| !! | GAAGAAGCTGGTTAATTTGT+TGG | + | chr5.1:68916765-68916784 | MS.gene99415:CDS | 35.0% |
| !! | GAAGAAGTGTCTAAAAGGAA+GGG | + | chr5.1:68916580-68916599 | MS.gene99415:CDS | 35.0% |
| !! | TGAAGAAGTGTCTAAAAGGA+AGG | + | chr5.1:68916579-68916598 | MS.gene99415:CDS | 35.0% |
| !! | TTTGTTGGTGCTTGTGTTTT+TGG | + | chr5.1:68916780-68916799 | MS.gene99415:CDS | 35.0% |
| !! | TTTTTCTGGTCAGAAACTTG+AGG | - | chr5.1:68916526-68916545 | None:intergenic | 35.0% |
| !!! | GTTGTTGATTTTGTTGGTGT+TGG | - | chr5.1:68916442-68916461 | None:intergenic | 35.0% |
| AATTTGAACCACCCTCTGAT+TGG | + | chr5.1:68915947-68915966 | MS.gene99415:CDS | 40.0% | |
| AGTTAAGAAGCAGAAGAAGC+TGG | + | chr5.1:68916753-68916772 | MS.gene99415:CDS | 40.0% | |
| ATTTGAACCACCCTCTGATT+GGG | + | chr5.1:68915948-68915967 | MS.gene99415:CDS | 40.0% | |
| GTGTCTAAAAGGAAGGGAAA+AGG | + | chr5.1:68916586-68916605 | MS.gene99415:CDS | 40.0% | |
| TAGGATTCCCGCTTCAAATT+TGG | - | chr5.1:68916377-68916396 | None:intergenic | 40.0% | |
| ! | GAATGCATTATGTGGTTTGG+TGG | + | chr5.1:68916726-68916745 | MS.gene99415:CDS | 40.0% |
| ! | TGTTTCTGAGGAATTGTTGC+TGG | - | chr5.1:68916493-68916512 | None:intergenic | 40.0% |
| !! | ATTGGTGCCAAATTTGAAGC+GGG | + | chr5.1:68916367-68916386 | MS.gene99415:CDS | 40.0% |
| !! | CATTGGTGCCAAATTTGAAG+CGG | + | chr5.1:68916366-68916385 | MS.gene99415:CDS | 40.0% |
| !!! | GTGTTTTTGGTCTTGCTGTT+TGG | + | chr5.1:68916793-68916812 | MS.gene99415:CDS | 40.0% |
| ATCCTCATGATCCCAATCAG+AGG | - | chr5.1:68915962-68915981 | None:intergenic | 45.0% | |
| ATGCTGCTAGTGTCAGAATG+AGG | - | chr5.1:68916349-68916368 | None:intergenic | 45.0% | |
| CATTCTGACACTAGCAGCAT+TGG | + | chr5.1:68916349-68916368 | MS.gene99415:CDS | 45.0% | |
| GAAGGGAAAAGGTAAAGCTG+AGG | + | chr5.1:68916597-68916616 | MS.gene99415:CDS | 45.0% | |
| TCCTCATGATCCCAATCAGA+GGG | - | chr5.1:68915961-68915980 | None:intergenic | 45.0% | |
| ! | GGAATTGTTGCTGGTTGTTG+AGG | - | chr5.1:68916484-68916503 | None:intergenic | 45.0% |
| !!! | GGAGCAGTGTTTTGTTTCTG+AGG | - | chr5.1:68916505-68916524 | None:intergenic | 45.0% |
| AACCTCAACCCGTGTGCTAA+GGG | + | chr5.1:68916258-68916277 | MS.gene99415:CDS | 50.0% | |
| ACCACTAACCCTTAGCACAC+GGG | - | chr5.1:68916269-68916288 | None:intergenic | 50.0% | |
| ACCCTCTGATTGGGATCATG+AGG | + | chr5.1:68915957-68915976 | MS.gene99415:CDS | 50.0% | |
| CACCACTAACCCTTAGCACA+CGG | - | chr5.1:68916270-68916289 | None:intergenic | 50.0% | |
| CTGCTAGTGTCAGAATGAGG+TGG | - | chr5.1:68916346-68916365 | None:intergenic | 50.0% | |
| TAACCTCAACCCGTGTGCTA+AGG | + | chr5.1:68916257-68916276 | MS.gene99415:CDS | 50.0% | |
| TCATGATCCCAATCAGAGGG+TGG | - | chr5.1:68915958-68915977 | None:intergenic | 50.0% | |
| ! | GCTGGTTGTTGAGGCTTTTG+AGG | - | chr5.1:68916475-68916494 | None:intergenic | 50.0% |
| ACCCGTGTGCTAAGGGTTAG+TGG | + | chr5.1:68916265-68916284 | MS.gene99415:CDS | 55.0% | |
| ! | AACCCTTAGCACACGGGTTG+AGG | - | chr5.1:68916263-68916282 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 68915901 | 68916879 | 68915901 | ID=MS.gene99415 |
| chr5.1 | mRNA | 68915901 | 68916879 | 68915901 | ID=MS.gene99415.t1;Parent=MS.gene99415 |
| chr5.1 | exon | 68915901 | 68916012 | 68915901 | ID=MS.gene99415.t1.exon1;Parent=MS.gene99415.t1 |
| chr5.1 | CDS | 68915901 | 68916012 | 68915901 | ID=cds.MS.gene99415.t1;Parent=MS.gene99415.t1 |
| chr5.1 | exon | 68916227 | 68916879 | 68916227 | ID=MS.gene99415.t1.exon2;Parent=MS.gene99415.t1 |
| chr5.1 | CDS | 68916227 | 68916879 | 68916227 | ID=cds.MS.gene99415.t1;Parent=MS.gene99415.t1 |
| Gene Sequence |
| Protein sequence |
