Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99916.t1 | AES87914.2 | 79.6 | 137 | 18 | 1 | 1 | 137 | 1 | 127 | 3.40E-48 | 201.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99916.t1 | G7JSH2 | 79.6 | 137 | 18 | 1 | 1 | 137 | 1 | 127 | 2.5e-48 | 201.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene99916 | 0.803939 | 2.74E-49 | -1.69E-46 |
| MS.gene050584 | MS.gene99916 | 0.819993 | 8.68E-53 | -1.69E-46 |
| MS.gene050585 | MS.gene99916 | 0.82615 | 3.18E-54 | -1.69E-46 |
| MS.gene050587 | MS.gene99916 | 0.807325 | 5.34E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene99916 | 0.804819 | 1.80E-49 | -1.69E-46 |
| MS.gene052142 | MS.gene99916 | 0.812418 | 4.28E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene99916 | 0.833633 | 4.77E-56 | -1.69E-46 |
| MS.gene05456 | MS.gene99916 | 0.806788 | 6.94E-50 | -1.69E-46 |
| MS.gene055359 | MS.gene99916 | 0.816438 | 5.53E-52 | -1.69E-46 |
| MS.gene059006 | MS.gene99916 | 0.829513 | 4.93E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99916.t1 | MTR_4g037480 | 87.591 | 137 | 7 | 1 | 1 | 137 | 1 | 127 | 2.86e-70 | 207 |
| MS.gene99916.t1 | MTR_4g037560 | 83.453 | 139 | 20 | 2 | 1 | 137 | 1 | 138 | 4.12e-62 | 187 |
| MS.gene99916.t1 | MTR_2g090185 | 54.348 | 138 | 50 | 5 | 1 | 137 | 1 | 126 | 2.48e-30 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99916.t1 | AT2G20870 | 38.333 | 120 | 67 | 5 | 23 | 137 | 23 | 140 | 3.54e-13 | 62.8 |
Find 51 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCTAATAATCCATTTAT+TGG | 0.205431 | 4.2:-57431151 | MS.gene99916:CDS |
| TACCCTGGCATTGGACATTT+AGG | 0.235381 | 4.2:-57431196 | MS.gene99916:CDS |
| GTCGTCATTGTCGGAGTTCT+TGG | 0.244678 | 4.2:+57431267 | None:intergenic |
| CCTCCGGTGCCACCAATAAA+TGG | 0.251778 | 4.2:+57431139 | None:intergenic |
| GGAGGATCAGGTTCGGGTTT+AGG | 0.274000 | 4.2:-57431121 | MS.gene99916:CDS |
| CCGCCACCACTGCCGGGATT+TGG | 0.287982 | 4.2:+57431007 | None:intergenic |
| CTCTTTCTTGTCGTCATTGT+CGG | 0.295441 | 4.2:+57431258 | None:intergenic |
| CCACCAATAAATGGATTATT+AGG | 0.325480 | 4.2:+57431148 | None:intergenic |
| CCCGGCAGTGGTGGCGGCGG+TGG | 0.329857 | 4.2:-57431001 | MS.gene99916:CDS |
| TTAGGATTTCCACCGAAATT+CGG | 0.332231 | 4.2:-57431178 | MS.gene99916:CDS |
| TAGGATTTCCACCGAAATTC+GGG | 0.357846 | 4.2:-57431177 | MS.gene99916:CDS |
| TGTTCCAAACCCTGGCTTTG+AGG | 0.364651 | 4.2:-57431032 | MS.gene99916:CDS |
| GGAACCTCAAAGCCAGGGTT+TGG | 0.367827 | 4.2:+57431028 | None:intergenic |
| GGCACCGGAGGATCAGGTTC+GGG | 0.375117 | 4.2:-57431127 | MS.gene99916:CDS |
| GGCAGTGGTGGCGGCGGTGG+TGG | 0.379041 | 4.2:-57430998 | MS.gene99916:CDS |
| TGGCACCGGAGGATCAGGTT+CGG | 0.389708 | 4.2:-57431128 | MS.gene99916:CDS |
| ATTGGTGGCACCGGAGGATC+AGG | 0.409486 | 4.2:-57431133 | MS.gene99916:CDS |
| GGCTTTGAGGTTCCAAATCC+CGG | 0.426165 | 4.2:-57431019 | MS.gene99916:CDS |
| TCAGGTTCGGGTTTAGGATC+AGG | 0.431514 | 4.2:-57431115 | MS.gene99916:CDS |
| ACCACCGCCGCCACCACTGC+CGG | 0.432310 | 4.2:+57431000 | None:intergenic |
| TCAGGTCATAGTTATGTTCC+CGG | 0.442709 | 4.2:-57431070 | MS.gene99916:CDS |
| TTAGGAGTAAGCCCGAATTT+CGG | 0.445449 | 4.2:+57431166 | None:intergenic |
| ACAACACGGCAAGCAGTTGC+TGG | 0.458864 | 4.2:-57431301 | MS.gene99916:CDS |
| CCAAATCCCGGCAGTGGTGG+CGG | 0.459199 | 4.2:-57431007 | MS.gene99916:CDS |
| TCAGGAACAGGAGCAGGATC+AGG | 0.471562 | 4.2:-57431097 | MS.gene99916:CDS |
| GGAGCAGGATCAGGATCATC+AGG | 0.472228 | 4.2:-57431088 | MS.gene99916:CDS |
| GGATTTGGAACCTCAAAGCC+AGG | 0.493115 | 4.2:+57431022 | None:intergenic |
| CCTAATAATCCATTTATTGG+TGG | 0.494125 | 4.2:-57431148 | MS.gene99916:CDS |
| AACAAATGTGTCATCGCCAC+CGG | 0.495697 | 4.2:+57431051 | None:intergenic |
| AATCCTAAATGTCCAATGCC+AGG | 0.501871 | 4.2:+57431193 | None:intergenic |
| CGGAGTTCTTGGCAATGTTG+CGG | 0.505040 | 4.2:+57431278 | None:intergenic |
| TCGGGTTTAGGATCAGGAAC+AGG | 0.510472 | 4.2:-57431109 | MS.gene99916:CDS |
| GAGGTTCCAAATCCCGGCAG+TGG | 0.510808 | 4.2:-57431013 | MS.gene99916:CDS |
| CCACCGCCGCCACCACTGCC+GGG | 0.517941 | 4.2:+57431001 | None:intergenic |
| AATCCATTTATTGGTGGCAC+CGG | 0.519391 | 4.2:-57431142 | MS.gene99916:CDS |
| AAACTACATTACCCTGGCAT+TGG | 0.520118 | 4.2:-57431205 | MS.gene99916:CDS |
| CCATTTATTGGTGGCACCGG+AGG | 0.520418 | 4.2:-57431139 | MS.gene99916:CDS |
| AATCCCGGCAGTGGTGGCGG+CGG | 0.526722 | 4.2:-57431004 | MS.gene99916:CDS |
| TAAACCCGAACCTGATCCTC+CGG | 0.533500 | 4.2:+57431123 | None:intergenic |
| TTAGGATCAGGAACAGGAGC+AGG | 0.546096 | 4.2:-57431103 | MS.gene99916:CDS |
| GACACATTTGTTCCAAACCC+TGG | 0.566936 | 4.2:-57431040 | MS.gene99916:CDS |
| GTTCCAAATCCCGGCAGTGG+TGG | 0.572010 | 4.2:-57431010 | MS.gene99916:CDS |
| GGAGTAAGCCCGAATTTCGG+TGG | 0.586534 | 4.2:+57431169 | None:intergenic |
| ACAAATGTGTCATCGCCACC+GGG | 0.594099 | 4.2:+57431052 | None:intergenic |
| CTGCTTGCCGTGTTGTGACA+AGG | 0.611217 | 4.2:+57431308 | None:intergenic |
| ATCCTAAATGTCCAATGCCA+GGG | 0.617878 | 4.2:+57431194 | None:intergenic |
| GATTTGGAACCTCAAAGCCA+GGG | 0.635813 | 4.2:+57431023 | None:intergenic |
| ATATCCTCCTTGTCACAACA+CGG | 0.657664 | 4.2:-57431315 | MS.gene99916:CDS |
| CATGGTAAACTACATTACCC+TGG | 0.662493 | 4.2:-57431211 | MS.gene99916:CDS |
| GGTCATAGTTATGTTCCCGG+TGG | 0.737137 | 4.2:-57431067 | MS.gene99916:CDS |
| CTTGCCGTGTTGTGACAAGG+AGG | 0.782718 | 4.2:+57431311 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTCCTAATAATCCATTTAT+TGG | - | chr4.2:57431180-57431199 | MS.gene99916:CDS | 25.0% |
| !! | TTTTTATTCAAGCATGATCA+TGG | - | chr4.2:57431102-57431121 | MS.gene99916:CDS | 25.0% |
| CCTAATAATCCATTTATTGG+TGG | - | chr4.2:57431183-57431202 | MS.gene99916:CDS | 30.0% | |
| TCATGCTTGAATAAAAACTG+CGG | + | chr4.2:57431099-57431118 | None:intergenic | 30.0% | |
| !! | CCACCAATAAATGGATTATT+AGG | + | chr4.2:57431186-57431205 | None:intergenic | 30.0% |
| TTAGGATTTCCACCGAAATT+CGG | - | chr4.2:57431153-57431172 | MS.gene99916:CDS | 35.0% | |
| AAACTACATTACCCTGGCAT+TGG | - | chr4.2:57431126-57431145 | MS.gene99916:CDS | 40.0% | |
| AATCCTAAATGTCCAATGCC+AGG | + | chr4.2:57431141-57431160 | None:intergenic | 40.0% | |
| ATATCCTCCTTGTCACAACA+CGG | - | chr4.2:57431016-57431035 | MS.gene99916:CDS | 40.0% | |
| ATCCTAAATGTCCAATGCCA+GGG | + | chr4.2:57431140-57431159 | None:intergenic | 40.0% | |
| CATGGTAAACTACATTACCC+TGG | - | chr4.2:57431120-57431139 | MS.gene99916:CDS | 40.0% | |
| CTCTTTCTTGTCGTCATTGT+CGG | + | chr4.2:57431076-57431095 | None:intergenic | 40.0% | |
| TAGGATTTCCACCGAAATTC+GGG | - | chr4.2:57431154-57431173 | MS.gene99916:CDS | 40.0% | |
| TCAGGTCATAGTTATGTTCC+CGG | - | chr4.2:57431261-57431280 | MS.gene99916:CDS | 40.0% | |
| TTAGGAGTAAGCCCGAATTT+CGG | + | chr4.2:57431168-57431187 | None:intergenic | 40.0% | |
| ! | AATCCATTTATTGGTGGCAC+CGG | - | chr4.2:57431189-57431208 | MS.gene99916:CDS | 40.0% |
| AACAAATGTGTCATCGCCAC+CGG | + | chr4.2:57431283-57431302 | None:intergenic | 45.0% | |
| GACACATTTGTTCCAAACCC+TGG | - | chr4.2:57431291-57431310 | MS.gene99916:CDS | 45.0% | |
| ! | GATTTGGAACCTCAAAGCCA+GGG | + | chr4.2:57431311-57431330 | None:intergenic | 45.0% |
| ! | TACCCTGGCATTGGACATTT+AGG | - | chr4.2:57431135-57431154 | MS.gene99916:CDS | 45.0% |
| ACAAATGTGTCATCGCCACC+GGG | + | chr4.2:57431282-57431301 | None:intergenic | 50.0% | |
| CGGAGTTCTTGGCAATGTTG+CGG | + | chr4.2:57431056-57431075 | None:intergenic | 50.0% | |
| GGTCATAGTTATGTTCCCGG+TGG | - | chr4.2:57431264-57431283 | MS.gene99916:CDS | 50.0% | |
| GTCGTCATTGTCGGAGTTCT+TGG | + | chr4.2:57431067-57431086 | None:intergenic | 50.0% | |
| TAAACCCGAACCTGATCCTC+CGG | + | chr4.2:57431211-57431230 | None:intergenic | 50.0% | |
| TTAGGATCAGGAACAGGAGC+AGG | - | chr4.2:57431228-57431247 | MS.gene99916:CDS | 50.0% | |
| ! | GGATTTGGAACCTCAAAGCC+AGG | + | chr4.2:57431312-57431331 | None:intergenic | 50.0% |
| ! | TCAGGTTCGGGTTTAGGATC+AGG | - | chr4.2:57431216-57431235 | MS.gene99916:CDS | 50.0% |
| ! | TCGGGTTTAGGATCAGGAAC+AGG | - | chr4.2:57431222-57431241 | MS.gene99916:CDS | 50.0% |
| !! | GGCTTTGAGGTTCCAAATCC+CGG | - | chr4.2:57431312-57431331 | MS.gene99916:CDS | 50.0% |
| !! | TGTTCCAAACCCTGGCTTTG+AGG | - | chr4.2:57431299-57431318 | MS.gene99916:CDS | 50.0% |
| ACAACACGGCAAGCAGTTGC+TGG | - | chr4.2:57431030-57431049 | MS.gene99916:CDS | 55.0% | |
| CTGCTTGCCGTGTTGTGACA+AGG | + | chr4.2:57431026-57431045 | None:intergenic | 55.0% | |
| CTTGCCGTGTTGTGACAAGG+AGG | + | chr4.2:57431023-57431042 | None:intergenic | 55.0% | |
| GGAACCTCAAAGCCAGGGTT+TGG | + | chr4.2:57431306-57431325 | None:intergenic | 55.0% | |
| GGAGCAGGATCAGGATCATC+AGG | - | chr4.2:57431243-57431262 | MS.gene99916:CDS | 55.0% | |
| GGAGTAAGCCCGAATTTCGG+TGG | + | chr4.2:57431165-57431184 | None:intergenic | 55.0% | |
| TCAGGAACAGGAGCAGGATC+AGG | - | chr4.2:57431234-57431253 | MS.gene99916:CDS | 55.0% | |
| ! | CCATTTATTGGTGGCACCGG+AGG | - | chr4.2:57431192-57431211 | MS.gene99916:CDS | 55.0% |
| ! | GGAGGATCAGGTTCGGGTTT+AGG | - | chr4.2:57431210-57431229 | MS.gene99916:CDS | 55.0% |
| !! | CCTCCGGTGCCACCAATAAA+TGG | + | chr4.2:57431195-57431214 | None:intergenic | 55.0% |
| GAGGTTCCAAATCCCGGCAG+TGG | - | chr4.2:57431318-57431337 | MS.gene99916:CDS | 60.0% | |
| GTTCCAAATCCCGGCAGTGG+TGG | - | chr4.2:57431321-57431340 | MS.gene99916:CDS | 60.0% | |
| ! | ATTGGTGGCACCGGAGGATC+AGG | - | chr4.2:57431198-57431217 | MS.gene99916:CDS | 60.0% |
| ! | TGGCACCGGAGGATCAGGTT+CGG | - | chr4.2:57431203-57431222 | MS.gene99916:CDS | 60.0% |
| CCAAATCCCGGCAGTGGTGG+CGG | - | chr4.2:57431324-57431343 | MS.gene99916:CDS | 65.0% | |
| ! | GGCACCGGAGGATCAGGTTC+GGG | - | chr4.2:57431204-57431223 | MS.gene99916:CDS | 65.0% |
| CCGCCACCACTGCCGGGATT+TGG | + | chr4.2:57431327-57431346 | None:intergenic | 70.0% | |
| !! | AATCCCGGCAGTGGTGGCGG+CGG | - | chr4.2:57431327-57431346 | MS.gene99916:CDS | 70.0% |
| ACCACCGCCGCCACCACTGC+CGG | + | chr4.2:57431334-57431353 | None:intergenic | 75.0% | |
| ! | CCACCGCCGCCACCACTGCC+GGG | + | chr4.2:57431333-57431352 | None:intergenic | 80.0% |
| !!! | GGCAGTGGTGGCGGCGGTGG+TGG | - | chr4.2:57431333-57431352 | MS.gene99916:CDS | 80.0% |
| !!! | CCCGGCAGTGGTGGCGGCGG+TGG | - | chr4.2:57431330-57431349 | MS.gene99916:CDS | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 57430970 | 57431383 | 57430970 | ID=MS.gene99916 |
| chr4.2 | mRNA | 57430970 | 57431383 | 57430970 | ID=MS.gene99916.t1;Parent=MS.gene99916 |
| chr4.2 | exon | 57430970 | 57431383 | 57430970 | ID=MS.gene99916.t1.exon1;Parent=MS.gene99916.t1 |
| chr4.2 | CDS | 57430970 | 57431383 | 57430970 | ID=cds.MS.gene99916.t1;Parent=MS.gene99916.t1 |
| Gene Sequence |
| Protein sequence |
