Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080047949.01.T01 | KEH40282.1 | 75 | 80 | 17 | 2 | 5 | 81 | 114 | 193 | 8.05E-26 | 105 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080047949.01.T01 | A0A072VEQ9 | 75.000 | 80 | 17 | 2 | 5 | 81 | 114 | 193 | 3.85e-26 | 105 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047949.01 | MsG0180000470.01 | 0.806968 | 6.358062e-50 | 3.498304e-47 |
| MsG0080047949.01 | MsG0480018318.01 | 0.802770 | 4.792865e-49 | 2.367787e-46 |
| MsG0080047949.01 | MsG0480022615.01 | -0.806656 | 7.398013e-50 | 4.037639e-47 |
| MsG0080047949.01 | MsG0780040576.01 | -0.824108 | 9.651961e-54 | 8.468855e-51 |
| MsG0080047949.01 | MsG0880044323.01 | 0.806976 | 6.330758e-50 | 3.484045e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080047949.01.T01 | MTR_1g026760 | 75.000 | 80 | 17 | 2 | 5 | 81 | 114 | 193 | 9.75e-30 | 105 |
| MsG0080047949.01.T01 | MTR_1g026760 | 65.306 | 49 | 17 | 0 | 37 | 85 | 114 | 162 | 2.97e-13 | 62.8 |
| MsG0080047949.01.T01 | MTR_2g010820 | 47.368 | 114 | 44 | 3 | 6 | 103 | 122 | 235 | 2.11e-15 | 70.5 |
| MsG0080047949.01.T01 | MTR_2g010820 | 48.039 | 102 | 43 | 3 | 6 | 98 | 231 | 331 | 1.40e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATCATATATATGCAAAA+AGG | 0.363965 | contig152end:-296685 | None:intergenic |
| GCACTATCAGTTTCAATAAC+AGG | 0.380019 | contig152end:-296513 | None:intergenic |
| AGTGCTAGTAATCATATTGA+TGG | 0.429815 | contig152end:+296531 | MsG0080047949.01.T01:CDS |
| GTCAGATTACAGATCCTTGT+TGG | 0.460468 | contig152end:+296575 | MsG0080047949.01.T01:CDS |
| GGCAGATTATGAATCCTTGT+TGG | 0.493238 | contig152end:+296479 | MsG0080047949.01.T01:CDS |
| GATTCTGATGTTGAAATTGT+CGG | 0.493824 | contig152end:+296702 | MsG0080047949.01.T01:CDS |
| AGTGCTAGTAATCATAATGA+TGG | 0.528400 | contig152end:+296627 | MsG0080047949.01.T01:CDS |
| TGGTTCAAAATTTGATGAAG+TGG | 0.540993 | contig152end:+296647 | MsG0080047949.01.T01:CDS |
| GATGTTGAAATTGTCGGCTA+TGG | 0.575971 | contig152end:+296708 | MsG0080047949.01.T01:CDS |
| ATACTTTCGGGTGAATGTAC+CGG | 0.596441 | contig152end:+296439 | None:intergenic |
| AGGATCATATTTAGCCAACA+AGG | 0.632299 | contig152end:-296493 | None:intergenic |
| GTACCGGTGTCATGATGAAG+TGG | 0.633005 | contig152end:+296455 | MsG0080047949.01.T01:CDS |
| TGGTTCAAGCAATGATGAAG+TGG | 0.638985 | contig152end:+296551 | MsG0080047949.01.T01:CDS |
| AGCATTATATTTATCCAACA+AGG | 0.642252 | contig152end:-296589 | None:intergenic |
| CCTCCACTTCATCATGACAC+CGG | 0.679540 | contig152end:-296458 | None:intergenic |
| CCGGTGTCATGATGAAGTGG+AGG | 0.693197 | contig152end:+296458 | MsG0080047949.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAATCATATATATGCAAAA+AGG | - | contig152end:296688-296707 | None:intergenic | 20.0% |
| ! | AGCATTATATTTATCCAACA+AGG | - | contig152end:296592-296611 | None:intergenic | 25.0% |
| AGTGCTAGTAATCATAATGA+TGG | + | contig152end:296627-296646 | MsG0080047949.01.T01:CDS | 30.0% | |
| AGTGCTAGTAATCATATTGA+TGG | + | contig152end:296531-296550 | MsG0080047949.01.T01:CDS | 30.0% | |
| GATTCTGATGTTGAAATTGT+CGG | + | contig152end:296702-296721 | MsG0080047949.01.T01:CDS | 30.0% | |
| TGGTTCAAAATTTGATGAAG+TGG | + | contig152end:296647-296666 | MsG0080047949.01.T01:CDS | 30.0% | |
| AGGATCATATTTAGCCAACA+AGG | - | contig152end:296496-296515 | None:intergenic | 35.0% | |
| GCACTATCAGTTTCAATAAC+AGG | - | contig152end:296516-296535 | None:intergenic | 35.0% | |
| GGCAGATTATGAATCCTTGT+TGG | + | contig152end:296479-296498 | MsG0080047949.01.T01:CDS | 40.0% | |
| GTCAGATTACAGATCCTTGT+TGG | + | contig152end:296575-296594 | MsG0080047949.01.T01:CDS | 40.0% | |
| TGGTTCAAGCAATGATGAAG+TGG | + | contig152end:296551-296570 | MsG0080047949.01.T01:CDS | 40.0% | |
| ! | GATGTTGAAATTGTCGGCTA+TGG | + | contig152end:296708-296727 | MsG0080047949.01.T01:CDS | 40.0% |
| CCTCCACTTCATCATGACAC+CGG | - | contig152end:296461-296480 | None:intergenic | 50.0% | |
| !! | GTACCGGTGTCATGATGAAG+TGG | + | contig152end:296455-296474 | MsG0080047949.01.T01:CDS | 50.0% |
| !! | CCGGTGTCATGATGAAGTGG+AGG | + | contig152end:296458-296477 | MsG0080047949.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig152end | gene | 296453 | 296770 | 296453 | ID=MsG0080047949.01;Name=MsG0080047949.01 |
| contig152end | mRNA | 296453 | 296770 | 296453 | ID=MsG0080047949.01.T01;Parent=MsG0080047949.01;Name=MsG0080047949.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|105 |
| contig152end | exon | 296453 | 296770 | 296453 | ID=MsG0080047949.01.T01:exon:13704;Parent=MsG0080047949.01.T01 |
| contig152end | CDS | 296453 | 296770 | 296453 | ID=MsG0080047949.01.T01:cds;Parent=MsG0080047949.01.T01 |
| Gene Sequence |
| Protein sequence |
