AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048497.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048497.01.T01 MTR_3g046320 100.000 176 0 0 1 176 1 176 5.09e-132 367
MsG0080048497.01.T01 MTR_3g046320 99.425 174 1 0 3 176 5 178 8.60e-130 361
MsG0080048497.01.T01 MTR_5g091135 95.402 174 8 0 3 176 5 178 3.17e-124 347
MsG0080048497.01.T01 MTR_5g091120 95.402 174 8 0 3 176 5 178 3.17e-124 347
MsG0080048497.01.T01 MTR_2g098000 95.402 174 8 0 3 176 5 178 3.17e-124 347
MsG0080048497.01.T01 MTR_2g019720 77.273 66 15 0 90 155 1 66 1.31e-32 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048497.01.T01 AT2G34480 89.655 174 18 0 3 176 44 217 3.76e-118 333
MsG0080048497.01.T01 AT2G34480 89.655 174 18 0 3 176 5 178 6.05e-118 331
MsG0080048497.01.T01 AT1G29965 89.535 172 18 0 5 176 7 178 8.33e-117 328
MsG0080048497.01.T01 AT3G14600 87.931 174 21 0 3 176 5 178 9.41e-116 326
MsG0080048497.01.T01 AT1G29970 89.535 172 18 0 5 176 141 312 5.35e-114 326

Find 54 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTAAGAACTACGGAATT+TGG 0.227031 contig337end:-2402 MsG0080048497.01.T01:CDS
TACCGCGACACTACACTAAA+TGG 0.308319 contig337end:-2334 MsG0080048497.01.T01:CDS
TTGTCTTGAGCTTCCTGGTT+GGG 0.316740 contig337end:+2140 MsG0080048497.01.T01:intergenic
GTATGTTGTCTTGAGCTTCC+TGG 0.318485 contig337end:+2135 MsG0080048497.01.T01:intergenic
CTTTGGTGTTCAAGAAGATT+AGG 0.360558 contig337end:-2165 MsG0080048497.01.T01:CDS
GACTTACCAGAACTTGGATT+TGG 0.361331 contig337end:+5029 MsG0080048497.01.T01:intergenic
CTTGTACATGTTGTGATATC+CGG 0.368635 contig337end:+2360 MsG0080048497.01.T01:intergenic
CTCGAAAATCAAGTTTCCTT+TGG 0.369229 contig337end:-2182 MsG0080048497.01.T01:CDS
AATTCCGTAGTTCTTAATCT+TGG 0.384261 contig337end:+2405 MsG0080048497.01.T01:intergenic
AACTTCATCCTATAGATCTT+AGG 0.390754 contig337end:+5075 MsG0080048497.01.T01:intergenic
TTACATAAACATGTTGGGTT+TGG 0.393552 contig337end:+2105 MsG0080048497.01.T01:intergenic
CGAAATACATACCTCATTGA+TGG 0.398520 contig337end:+4631 MsG0080048497.01.T01:intergenic
ATGAACATCCTAAGATCTAT+AGG 0.408451 contig337end:-5083 MsG0080048497.01.T01:CDS
TGTCTTGAGCTTCCTGGTTG+GGG 0.409521 contig337end:+2141 MsG0080048497.01.T01:intergenic
TCCCAGCTAACCTTTGCAAA+AGG 0.413105 contig337end:-2228 MsG0080048497.01.T01:CDS
ATCATTGACTTACCAGAACT+TGG 0.415763 contig337end:+5023 MsG0080048497.01.T01:intergenic
GAGAATTACATAAACATGTT+GGG 0.416940 contig337end:+2100 MsG0080048497.01.T01:intergenic
TTGTGGCAGTCTTGATGATC+TGG 0.419281 contig337end:+2251 MsG0080048497.01.T01:intergenic
GGATTTGGCACGCACTTCAT+TGG 0.425117 contig337end:+5044 MsG0080048497.01.T01:intergenic
CTTCGATACCAGAGCCGAAC+CGG 0.434164 contig337end:-2379 MsG0080048497.01.T01:CDS
CCGTAGTTCTTAATCTTGGT+TGG 0.442883 contig337end:+2409 MsG0080048497.01.T01:intergenic
TGCGTGCCAAATCCAAGTTC+TGG 0.459519 contig337end:-5035 MsG0080048497.01.T01:intron
TGTGATATCCGGTTCGGCTC+TGG 0.459842 contig337end:+2371 MsG0080048497.01.T01:intergenic
TGAATATATGTACAATGAAA+TGG 0.482427 contig337end:-2305 MsG0080048497.01.T01:CDS
CCCAGCTAACCTTTGCAAAA+GGG 0.486205 contig337end:-2227 MsG0080048497.01.T01:CDS
GATCTATAGGATGAAGTTGT+GGG 0.489458 contig337end:-5070 MsG0080048497.01.T01:CDS
TGAGAATTACATAAACATGT+TGG 0.490122 contig337end:+2099 MsG0080048497.01.T01:intergenic
CTCTTCCCACAACCTGGTAC+TGG 0.493497 contig337end:+5124 MsG0080048497.01.T01:intergenic
ACATGTTGTGATATCCGGTT+CGG 0.502939 contig337end:+2365 MsG0080048497.01.T01:intergenic
TGGCTTCACGTCATAGAGTC+AGG 0.506716 contig337end:-2285 MsG0080048497.01.T01:CDS
TTTGGCACGCACTTCATTGG+TGG 0.507151 contig337end:+5047 MsG0080048497.01.T01:intergenic
GTTGTCTTGAGCTTCCTGGT+TGG 0.517024 contig337end:+2139 MsG0080048497.01.T01:intergenic
GTATCACCAGTACCAGGTTG+TGG 0.520361 contig337end:-5130 MsG0080048497.01.T01:CDS
AGATCTATAGGATGAAGTTG+TGG 0.528188 contig337end:-5071 MsG0080048497.01.T01:CDS
AGAAGGTTAAGAAGAGCAAT+GGG 0.531968 contig337end:-4664 MsG0080048497.01.T01:CDS
CGGATATCACAACATGTACA+AGG 0.534565 contig337end:-2359 MsG0080048497.01.T01:CDS
TATCACCAGTACCAGGTTGT+GGG 0.550528 contig337end:-5129 MsG0080048497.01.T01:CDS
GGTTAAGAAGAGCAATGGGC+AGG 0.558529 contig337end:-4660 MsG0080048497.01.T01:CDS
GCAAAGGTTAGCTGGGATTG+TGG 0.562321 contig337end:+2234 MsG0080048497.01.T01:intergenic
GGATGTTCATCCTTCTCAGT+AGG 0.562491 contig337end:+5096 MsG0080048497.01.T01:intergenic
GTCTTGATGATCTGGATGCA+TGG 0.564131 contig337end:+2259 MsG0080048497.01.T01:intergenic
GTCTTGAGCTTCCTGGTTGG+GGG 0.565257 contig337end:+2142 MsG0080048497.01.T01:intergenic
TTTCGAGTTATGGAACTGCT+TGG 0.570105 contig337end:+2198 MsG0080048497.01.T01:intergenic
AAGAAGGTTAAGAAGAGCAA+TGG 0.574791 contig337end:-4665 MsG0080048497.01.T01:CDS
TGCTGATTCGTTATAAGCGT+AGG 0.575138 contig337end:-2061 MsG0080048497.01.T01:three_prime_UTR
GAAGAGCTCTTCCCACAACC+TGG 0.580673 contig337end:+5118 MsG0080048497.01.T01:intergenic
CCAACCAAGATTAAGAACTA+CGG 0.592484 contig337end:-2409 MsG0080048497.01.T01:CDS
AAGAGCTCTTCCTACTGAGA+AGG 0.593469 contig337end:-5106 MsG0080048497.01.T01:CDS
CTAATCTTCTTGAACACCAA+AGG 0.593573 contig337end:+2166 MsG0080048497.01.T01:intergenic
GAATCAGTATCACCAGTACC+AGG 0.603715 contig337end:-5136 MsG0080048497.01.T01:CDS
GCGTAGGAAGAGCTTAGAGA+AGG 0.618322 contig337end:-2045 MsG0080048497.01.T01:three_prime_UTR
GAAGCTCAAGACAACATACA+AGG 0.626427 contig337end:-2131 MsG0080048497.01.T01:CDS
CACCATTTAGTGTAGTGTCG+CGG 0.677168 contig337end:+2332 MsG0080048497.01.T01:intergenic
GCAGGTGCTTGCCATCAATG+AGG 0.695053 contig337end:-4642 MsG0080048497.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATTAAAAAGAAAATTCT+TGG + contig337end:5129-5148 MsG0080048497.01.T01:intergenic 15.0%
!! TATATTATTTATAGAAAACC+AGG - contig337end:3541-3560 MsG0080048497.01.T01:intron 15.0%
!!! ACAAAGGTTATAAAATATTT+TGG - contig337end:4164-4183 MsG0080048497.01.T01:intron 15.0%
!!! TTTTTGTTTTGAAATTTTGT+AGG - contig337end:2345-2364 MsG0080048497.01.T01:CDS 15.0%
!! AACATTAAAAAAAACGAGTT+GGG + contig337end:3617-3636 MsG0080048497.01.T01:intergenic 20.0%
!! AAGCTATACATATATTGTTA+GGG - contig337end:4240-4259 MsG0080048497.01.T01:intron 20.0%
!! AATTAAAACCTAAAACTTTG+AGG + contig337end:4570-4589 MsG0080048497.01.T01:intergenic 20.0%
!! ACTCTATTAAAACATTTAGA+AGG - contig337end:2891-2910 MsG0080048497.01.T01:intron 20.0%
!! CTCTATTAAAACATTTAGAA+GGG - contig337end:2892-2911 MsG0080048497.01.T01:intron 20.0%
!! GAAATACAACAATAAAACAA+AGG + contig337end:2506-2525 MsG0080048497.01.T01:intergenic 20.0%
!! TAAGCTATACATATATTGTT+AGG - contig337end:4239-4258 MsG0080048497.01.T01:intron 20.0%
!! TATAATTTACTCTTCTTACT+AGG + contig337end:2838-2857 MsG0080048497.01.T01:intergenic 20.0%
!! TGAATATATGTACAATGAAA+TGG - contig337end:4746-4765 MsG0080048497.01.T01:intron 20.0%
!! TTAAACACAAACTTACAATA+GGG - contig337end:3772-3791 MsG0080048497.01.T01:intron 20.0%
!! TTATGCAATACTCTATTTAA+CGG + contig337end:4468-4487 MsG0080048497.01.T01:intergenic 20.0%
!! TTTAAACACAAACTTACAAT+AGG - contig337end:3771-3790 MsG0080048497.01.T01:intron 20.0%
!!! ATAAACTTTTACCTTTGTAA+TGG + contig337end:3242-3261 MsG0080048497.01.T01:intergenic 20.0%
!!! ATGAGAAAAATGAAATTTTC+TGG - contig337end:3516-3535 MsG0080048497.01.T01:intron 20.0%
!!! ATGTTAAAAAGTAGTATTGA+TGG - contig337end:3260-3279 MsG0080048497.01.T01:intron 20.0%
!!! GTTAAACTATACACATTTTA+CGG - contig337end:3941-3960 MsG0080048497.01.T01:intron 20.0%
!!! TGAAACATGAATTTTAACTA+AGG - contig337end:2771-2790 MsG0080048497.01.T01:intron 20.0%
!!! TGAGAAAAATGAAATTTTCT+GGG - contig337end:3517-3536 MsG0080048497.01.T01:intron 20.0%
!!! TGTTAAAAAGTAGTATTGAT+GGG - contig337end:3261-3280 MsG0080048497.01.T01:intron 20.0%
!!! TTGTTTTAATTTGCTCATTT+TGG - contig337end:5080-5099 MsG0080048497.01.T01:CDS 20.0%
! AGAAGAGTAAATTATAGCTA+AGG - contig337end:2842-2861 MsG0080048497.01.T01:intron 25.0%
! AGCTATACATATATTGTTAG+GGG - contig337end:4241-4260 MsG0080048497.01.T01:intron 25.0%
! AGGTAAATTATATCGAAGTT+TGG - contig337end:4270-4289 MsG0080048497.01.T01:intron 25.0%
! ATCAGTCATTTAACTAAGTA+CGG + contig337end:3826-3845 MsG0080048497.01.T01:intergenic 25.0%
! GAACATTAAAAAAAACGAGT+TGG + contig337end:3618-3637 MsG0080048497.01.T01:intergenic 25.0%
! GAATCATGCATCAAAAATTT+AGG - contig337end:4334-4353 MsG0080048497.01.T01:intron 25.0%
! GAGAATTACATAAACATGTT+GGG + contig337end:4954-4973 MsG0080048497.01.T01:intergenic 25.0%
! TGAGAATTACATAAACATGT+TGG + contig337end:4955-4974 MsG0080048497.01.T01:intergenic 25.0%
!! ATTACAATTTTACCCTTAAG+TGG + contig337end:3045-3064 MsG0080048497.01.T01:intergenic 25.0%
!! GAAAACGAAAACAAAGTATT+TGG - contig337end:3348-3367 MsG0080048497.01.T01:intron 25.0%
!! TTACAATTTTACCCTTAAGT+GGG + contig337end:3044-3063 MsG0080048497.01.T01:intergenic 25.0%
!! TTTGCATTGTTCTTGTTTAA+TGG - contig337end:2095-2114 MsG0080048497.01.T01:exon 25.0%
!!! AACATGAATTTTAACTAAGG+TGG - contig337end:2774-2793 MsG0080048497.01.T01:intron 25.0%
!!! AATCAATTTTTTATGTCCTC+TGG - contig337end:4204-4223 MsG0080048497.01.T01:intron 25.0%
!!! CTGATATTTTCTTTGATTTG+AGG + contig337end:4401-4420 MsG0080048497.01.T01:intergenic 25.0%
!!! TAAGATGGCAAAATTTTAAC+TGG - contig337end:2597-2616 MsG0080048497.01.T01:intron 25.0%
AAAAAACAAAATGCGATCAC+CGG + contig337end:2330-2349 MsG0080048496.01.T01:intergenic 30.0%
AACTTCATCCTATAGATCTT+AGG + contig337end:1979-1998 MsG0080048497.01.T01:intergenic 30.0%
AAGTGATTGAAAACACGTTT+AGG - contig337end:3408-3427 MsG0080048497.01.T01:intron 30.0%
AATTCCGTAGTTCTTAATCT+TGG + contig337end:4649-4668 MsG0080048497.01.T01:intergenic 30.0%
ACAATAAAACAAAGGCTTCA+TGG + contig337end:2498-2517 MsG0080048497.01.T01:intergenic 30.0%
AGAAAACGAAACAGAGTTAT+GGG - contig337end:3317-3336 MsG0080048497.01.T01:intron 30.0%
AGATTAAGAACTACGGAATT+TGG - contig337end:4649-4668 MsG0080048497.01.T01:CDS 30.0%
ATGAACATCCTAAGATCTAT+AGG - contig337end:1968-1987 MsG0080048497.01.T01:three_prime_UTR 30.0%
CAAGAATTGAGAAAAATGCA+TGG + contig337end:3880-3899 MsG0080048497.01.T01:intergenic 30.0%
CTCTTGCATATTCAAGAATA+TGG - contig337end:3973-3992 MsG0080048497.01.T01:intron 30.0%
GAGGATTCGAAAAAAAATAG+TGG - contig337end:2655-2674 MsG0080048497.01.T01:intron 30.0%
GATTCTCTGTTGTTAAACAT+TGG - contig337end:3843-3862 MsG0080048497.01.T01:intron 30.0%
GCAAAAACACAAAACATCAT+TGG + contig337end:2223-2242 MsG0080048497.01.T01:intergenic 30.0%
GTTTCAATTGCAGAAAATAG+CGG + contig337end:2757-2776 MsG0080048497.01.T01:intergenic 30.0%
TATATCGAAGTTTGGACATA+GGG - contig337end:4278-4297 MsG0080048497.01.T01:intron 30.0%
TTACATAAACATGTTGGGTT+TGG + contig337end:4949-4968 MsG0080048497.01.T01:intergenic 30.0%
TTAGTACCAAAGAATACAGA+AGG + contig337end:4018-4037 MsG0080048497.01.T01:intergenic 30.0%
TTATATCGAAGTTTGGACAT+AGG - contig337end:4277-4296 MsG0080048497.01.T01:intron 30.0%
TTCTGTATTCTTTGGTACTA+AGG - contig337end:4017-4036 MsG0080048497.01.T01:intron 30.0%
TTGTAAAATTGCAGCACAAT+AGG - contig337end:2704-2723 MsG0080048497.01.T01:intron 30.0%
TTGTTCATGAAAAGAGTTAC+CGG - contig337end:2308-2327 MsG0080048497.01.T01:CDS 30.0%
! AAACTTACAATAGGGCATTA+TGG - contig337end:3780-3799 MsG0080048497.01.T01:intron 30.0%
! AAGAAACTGTAGAACTTGTT+AGG - contig337end:4126-4145 MsG0080048497.01.T01:intron 30.0%
! CAAAGTATTTGGTTGAAAAC+AGG - contig337end:3359-3378 MsG0080048497.01.T01:intron 30.0%
! CATTTTTCTCAATTCTTGTC+AGG - contig337end:3882-3901 MsG0080048497.01.T01:intron 30.0%
! GTAAGTTTGTGTTTAAAGCA+AGG + contig337end:3768-3787 MsG0080048497.01.T01:intergenic 30.0%
! TTTGTTCTGTTGATTAGTTC+TGG - contig337end:2128-2147 MsG0080048497.01.T01:CDS 30.0%
!! AGAAACTGTAGAACTTGTTA+GGG - contig337end:4127-4146 MsG0080048497.01.T01:intron 30.0%
!! CATCTGCTTCATTTTTGTTT+TGG - contig337end:5054-5073 MsG0080048497.01.T01:CDS 30.0%
!! GTTCTAGTTTGTCTAATAGT+GGG + contig337end:4526-4545 MsG0080048497.01.T01:intergenic 30.0%
!! GTTTCTATGGATTGTCTTTT+GGG + contig337end:3213-3232 MsG0080048497.01.T01:intergenic 30.0%
!! TGTTTCTATGGATTGTCTTT+TGG + contig337end:3214-3233 MsG0080048497.01.T01:intergenic 30.0%
!! TGTTTTTGCATTGCTCTTTT+TGG - contig337end:2234-2253 MsG0080048497.01.T01:CDS 30.0%
!!! ATCGTTTTTTCCATGTTTGT+TGG + contig337end:3663-3682 MsG0080048497.01.T01:intergenic 30.0%
!!! GAAACTTGATTTTCGAGTTA+TGG + contig337end:4866-4885 MsG0080048497.01.T01:intergenic 30.0%
!!! GAATTTTAACTAAGGTGGTT+TGG - contig337end:2779-2798 MsG0080048497.01.T01:intron 30.0%
!!! TTTTTTAATGTTCGTGCATC+AGG - contig337end:3625-3644 MsG0080048497.01.T01:intron 30.0%
!!! TTTTTTGAGAAAGCTGAAGA+AGG - contig337end:2370-2389 MsG0080048497.01.T01:CDS 30.0%
AACATCTGCGATGAATAAGA+TGG - contig337end:2582-2601 MsG0080048497.01.T01:intron 35.0%
AAGAAGGTTAAGAAGAGCAA+TGG - contig337end:2386-2405 MsG0080048497.01.T01:CDS 35.0%
AAGAGAACGAAACAGAGTAA+TGG - contig337end:3288-3307 MsG0080048497.01.T01:intron 35.0%
ACAATAGGATTGCTATGTAG+TGG - contig337end:2719-2738 MsG0080048497.01.T01:intron 35.0%
ACGAAACAGAGTAATGGTTA+TGG - contig337end:3294-3313 MsG0080048497.01.T01:intron 35.0%
AGAAGGTTAAGAAGAGCAAT+GGG - contig337end:2387-2406 MsG0080048497.01.T01:CDS 35.0%
AGATCTATAGGATGAAGTTG+TGG - contig337end:1980-1999 MsG0080048497.01.T01:three_prime_UTR 35.0%
ATTGAAAACACGTTTAGGTG+AGG - contig337end:3413-3432 MsG0080048497.01.T01:intron 35.0%
ATTTAGGAGTAAGTCACTGT+TGG - contig337end:4350-4369 MsG0080048497.01.T01:intron 35.0%
CCAACCAAGATTAAGAACTA+CGG - contig337end:4642-4661 MsG0080048497.01.T01:intron 35.0%
CGAAATACATACCTCATTGA+TGG + contig337end:2423-2442 MsG0080048497.01.T01:intergenic 35.0%
CTAATCTTCTTGAACACCAA+AGG + contig337end:4888-4907 MsG0080048497.01.T01:intergenic 35.0%
CTCGAAAATCAAGTTTCCTT+TGG - contig337end:4869-4888 MsG0080048497.01.T01:intron 35.0%
CTTGTACATGTTGTGATATC+CGG + contig337end:4694-4713 MsG0080048497.01.T01:intergenic 35.0%
GAAACAACATGCCATTACAA+AGG - contig337end:3228-3247 MsG0080048497.01.T01:intron 35.0%
GAGAAAACGAAACAGAGTTA+TGG - contig337end:3316-3335 MsG0080048497.01.T01:intron 35.0%
GATCTATAGGATGAAGTTGT+GGG - contig337end:1981-2000 MsG0080048497.01.T01:three_prime_UTR 35.0%
GGAAAAAACGATTCAGAACA+AGG - contig337end:3671-3690 MsG0080048497.01.T01:intron 35.0%
TATATTGTTAGGGGTCATTG+AGG - contig337end:4250-4269 MsG0080048497.01.T01:intron 35.0%
TCCTAACATGCTGGTATTAA+GGG + contig337end:4075-4094 MsG0080048497.01.T01:intergenic 35.0%
TTGAAAACACGTTTAGGTGA+GGG - contig337end:3414-3433 MsG0080048497.01.T01:intron 35.0%
TTTAGGAGTAAGTCACTGTT+GGG - contig337end:4351-4370 MsG0080048497.01.T01:intron 35.0%
TTTGCAAATGTTGCTCGTAA+TGG - contig337end:2527-2546 MsG0080048497.01.T01:intron 35.0%
TTTGGATGGGATAAACATGT+TGG - contig337end:2252-2271 MsG0080048497.01.T01:CDS 35.0%
! ATGGAACATGATAGAGTACT+TGG - contig337end:3992-4011 MsG0080048497.01.T01:intron 35.0%
! GATTGCTATGTAGTGGAATT+TGG - contig337end:2726-2745 MsG0080048497.01.T01:intron 35.0%
! TTCTTTGGTACTAAGGTATC+AGG - contig337end:4024-4043 MsG0080048497.01.T01:intron 35.0%
! TTGCATTGCTCTTTTTGGAT+GGG - contig337end:2239-2258 MsG0080048497.01.T01:CDS 35.0%
! TTGCTCATTTTGGATTCGTT+CGG - contig337end:5090-5109 MsG0080048497.01.T01:CDS 35.0%
! TTTGCATTGCTCTTTTTGGA+TGG - contig337end:2238-2257 MsG0080048497.01.T01:CDS 35.0%
!! ATCATTGACTTACCAGAACT+TGG + contig337end:2031-2050 MsG0080048497.01.T01:intergenic 35.0%
!! CGTTCTAGTTTGTCTAATAG+TGG + contig337end:4527-4546 MsG0080048497.01.T01:intergenic 35.0%
!! CTTTGGTGTTCAAGAAGATT+AGG - contig337end:4886-4905 MsG0080048497.01.T01:intron 35.0%
!! GTAATGGCATGTTGTTTCTA+TGG + contig337end:3226-3245 MsG0080048497.01.T01:intergenic 35.0%
!! TCCTGACTACATTGGTTTAA+AGG + contig337end:2938-2957 MsG0080048497.01.T01:intergenic 35.0%
!! TTGTCTAATAGTGGGTATAG+CGG + contig337end:4518-4537 MsG0080048497.01.T01:intergenic 35.0%
!! TTTAGCTCACTTCCTTTTTC+CGG + contig337end:3385-3404 MsG0080048497.01.T01:intergenic 35.0%
!!! GATGGCAAAATTTTAACTGG+TGG - contig337end:2600-2619 MsG0080048497.01.T01:intron 35.0%
!!! TTTTTTCCATGTTTGTTGGC+AGG + contig337end:3659-3678 MsG0080048497.01.T01:intergenic 35.0%
AATTCGCCGGAAAAGATTCA+TGG - contig337end:3459-3478 MsG0080048497.01.T01:intron 40.0%
ACATGTTGTGATATCCGGTT+CGG + contig337end:4689-4708 MsG0080048497.01.T01:intergenic 40.0%
ACGAAACAGAGTTATGGGTA+TGG - contig337end:3322-3341 MsG0080048497.01.T01:intron 40.0%
CCGTAGTTCTTAATCTTGGT+TGG + contig337end:4645-4664 MsG0080048497.01.T01:intergenic 40.0%
CCTAACATGCTGGTATTAAG+GGG + contig337end:4074-4093 MsG0080048497.01.T01:intergenic 40.0%
CGGATATCACAACATGTACA+AGG - contig337end:4692-4711 MsG0080048497.01.T01:intron 40.0%
GAAGCTCAAGACAACATACA+AGG - contig337end:4920-4939 MsG0080048497.01.T01:intron 40.0%
GACTTACCAGAACTTGGATT+TGG + contig337end:2025-2044 MsG0080048497.01.T01:intergenic 40.0%
GCCTTTAAACCAATGTAGTC+AGG - contig337end:2934-2953 MsG0080048497.01.T01:intron 40.0%
GGAAAAAGGAAGTGAGCTAA+AGG - contig337end:3384-3403 MsG0080048497.01.T01:intron 40.0%
GGAGGAAGATTTAAGCACAA+AGG - contig337end:4148-4167 MsG0080048497.01.T01:intron 40.0%
GTATTTGGTTGAAAACAGGC+CGG - contig337end:3363-3382 MsG0080048497.01.T01:intron 40.0%
GTCCTAACATGCTGGTATTA+AGG + contig337end:4076-4095 MsG0080048497.01.T01:intergenic 40.0%
GTTCGTAAGAACCCACTTAA+GGG - contig337end:3030-3049 MsG0080048497.01.T01:intron 40.0%
GTTGCTCGTAATGGTAAGAT+AGG - contig337end:2536-2555 MsG0080048497.01.T01:intron 40.0%
! ACTTGGCCTTCTGTATTCTT+TGG - contig337end:4009-4028 MsG0080048497.01.T01:intron 40.0%
! ATTTTGGATTCGTTCGGAGT+TGG - contig337end:5096-5115 MsG0080048497.01.T01:CDS 40.0%
! GTGTTTAAAGCAAGGTAGTC+AGG + contig337end:3760-3779 MsG0080048497.01.T01:intergenic 40.0%
! GTTCTCAGCCTCAAAGTTTT+AGG - contig337end:4559-4578 MsG0080048497.01.T01:intron 40.0%
!! AACTGTAGAACTTGTTAGGG+AGG - contig337end:4130-4149 MsG0080048497.01.T01:intron 40.0%
!! TAGCCCAAACCAATTTTGAC+AGG - contig337end:3591-3610 MsG0080048497.01.T01:intron 40.0%
!! TGCTGATTCGTTATAAGCGT+AGG - contig337end:4990-5009 MsG0080048497.01.T01:intron 40.0%
!! TTTCGAGTTATGGAACTGCT+TGG + contig337end:4856-4875 MsG0080048497.01.T01:intergenic 40.0%
AACAAGGTCGAGGCATGATT+GGG - contig337end:3687-3706 MsG0080048497.01.T01:intron 45.0%
AACGATTCAGAACAAGGTCG+AGG - contig337end:3677-3696 MsG0080048497.01.T01:intron 45.0%
AAGACTTGTAGAAGGGCAGA+AGG - contig337end:2812-2831 MsG0080048497.01.T01:intron 45.0%
AAGAGCTCTTCCTACTGAGA+AGG - contig337end:1945-1964 MsG0080048497.01.T01:three_prime_UTR 45.0%
ACCAGCATGTTAGGACTAGA+CGG - contig337end:4080-4099 MsG0080048497.01.T01:intron 45.0%
ACCTAAACCTAGCACACGAA+CGG + contig337end:3488-3507 MsG0080048497.01.T01:intergenic 45.0%
ACGTGGAGAAGACTTGTAGA+AGG - contig337end:2804-2823 MsG0080048497.01.T01:intron 45.0%
AGTTCCTACGTAGGTTCGAT+TGG - contig337end:2961-2980 MsG0080048497.01.T01:intron 45.0%
ATCGAAGTTTGGACATAGGG+TGG - contig337end:4281-4300 MsG0080048497.01.T01:intron 45.0%
CACCATTTAGTGTAGTGTCG+CGG + contig337end:4722-4741 MsG0080048497.01.T01:intergenic 45.0%
CCCAGCTAACCTTTGCAAAA+GGG - contig337end:4824-4843 MsG0080048497.01.T01:intron 45.0%
CCCCTTAATACCAGCATGTT+AGG - contig337end:4071-4090 MsG0080048497.01.T01:intron 45.0%
CGTGGAGAAGACTTGTAGAA+GGG - contig337end:2805-2824 MsG0080048497.01.T01:intron 45.0%
CTTAAACATCTGCGATCCAG+AGG + contig337end:4223-4242 MsG0080048497.01.T01:intergenic 45.0%
CTTGTCAGGAATTCGACTCA+GGG - contig337end:3896-3915 MsG0080048497.01.T01:intron 45.0%
GAACTCGTTCCTGACTACAT+TGG + contig337end:2946-2965 MsG0080048497.01.T01:intergenic 45.0%
GAATCAGTATCACCAGTACC+AGG - contig337end:1915-1934 MsG0080048497.01.T01:three_prime_UTR 45.0%
GCTACAACGCTATTGCATAG+AGG - contig337end:2636-2655 MsG0080048497.01.T01:intron 45.0%
GGATGTTCATCCTTCTCAGT+AGG + contig337end:1958-1977 MsG0080048497.01.T01:intergenic 45.0%
GGTAGGGTCAAAAGATAACC+TGG + contig337end:3562-3581 MsG0080048497.01.T01:intergenic 45.0%
GGTTCGTAAGAACCCACTTA+AGG - contig337end:3029-3048 MsG0080048497.01.T01:intron 45.0%
GTATGTTGTCTTGAGCTTCC+TGG + contig337end:4919-4938 MsG0080048497.01.T01:intergenic 45.0%
GTGTAGGAACGTAAACACGA+GGG - contig337end:2987-3006 MsG0080048497.01.T01:intron 45.0%
GTTGAAAACAGGCCGGAAAA+AGG - contig337end:3370-3389 MsG0080048497.01.T01:intron 45.0%
TAAACACGAGGGACGTAACA+CGG - contig337end:2998-3017 MsG0080048497.01.T01:intron 45.0%
TACCGCGACACTACACTAAA+TGG - contig337end:4717-4736 MsG0080048497.01.T01:intron 45.0%
TATCACCAGTACCAGGTTGT+GGG - contig337end:1922-1941 MsG0080048497.01.T01:three_prime_UTR 45.0%
TCCCAGCTAACCTTTGCAAA+AGG - contig337end:4823-4842 MsG0080048497.01.T01:intron 45.0%
TCGTGTTTACGTTCCTACAC+CGG + contig337end:2987-3006 MsG0080048497.01.T01:intergenic 45.0%
TCTTGTCAGGAATTCGACTC+AGG - contig337end:3895-3914 MsG0080048497.01.T01:intron 45.0%
TGGGCCTGTCAAAATTGGTT+TGG + contig337end:3598-3617 MsG0080048497.01.T01:intergenic 45.0%
TTGTCTTGAGCTTCCTGGTT+GGG + contig337end:4914-4933 MsG0080048497.01.T01:intergenic 45.0%
! ACTAAGGTGGTTTGGATACG+TGG - contig337end:2787-2806 MsG0080048497.01.T01:intron 45.0%
! GGGCCTGTCAAAATTGGTTT+GGG + contig337end:3597-3616 MsG0080048497.01.T01:intergenic 45.0%
! GGTTATCTTTTGACCCTACC+CGG - contig337end:3562-3581 MsG0080048497.01.T01:intron 45.0%
! GTCTTGATGATCTGGATGCA+TGG + contig337end:4795-4814 MsG0080048497.01.T01:intergenic 45.0%
! TCCCTTTTGCAAAGGTTAGC+TGG + contig337end:4828-4847 MsG0080048497.01.T01:intergenic 45.0%
! TTGTGGCAGTCTTGATGATC+TGG + contig337end:4803-4822 MsG0080048497.01.T01:intergenic 45.0%
!! AATAGTGGGTATAGCGGTAG+TGG + contig337end:4512-4531 MsG0080048497.01.T01:intergenic 45.0%
!! CCCTTTTGCAAAGGTTAGCT+GGG + contig337end:4827-4846 MsG0080048497.01.T01:intergenic 45.0%
ACCGTTCGTGTGCTAGGTTT+AGG - contig337end:3484-3503 MsG0080048497.01.T01:intron 50.0%
CTCGTTCCTGCCAACAAACA+TGG - contig337end:3650-3669 MsG0080048497.01.T01:intron 50.0%
GAACAAGGTCGAGGCATGAT+TGG - contig337end:3686-3705 MsG0080048497.01.T01:intron 50.0%
GCGTAGGAAGAGCTTAGAGA+AGG - contig337end:5006-5025 MsG0080048497.01.T01:intron 50.0%
GGACGTAACACGGTTCGTAA+CGG - contig337end:3008-3027 MsG0080048497.01.T01:intron 50.0%
GGATTTGGCACGCACTTCAT+TGG + contig337end:2010-2029 MsG0080048497.01.T01:intergenic 50.0%
GGTGTAGGAACGTAAACACG+AGG - contig337end:2986-3005 MsG0080048497.01.T01:intron 50.0%
GGTTAAGAAGAGCAATGGGC+AGG - contig337end:2391-2410 MsG0080048497.01.T01:CDS 50.0%
GTATCACCAGTACCAGGTTG+TGG - contig337end:1921-1940 MsG0080048497.01.T01:three_prime_UTR 50.0%
GTTGTCTTGAGCTTCCTGGT+TGG + contig337end:4915-4934 MsG0080048497.01.T01:intergenic 50.0%
TAAACCTAGCACACGAACGG+TGG + contig337end:3485-3504 MsG0080048497.01.T01:intergenic 50.0%
TCAGGAACGAGTTCCTACGT+AGG - contig337end:2952-2971 MsG0080048497.01.T01:intron 50.0%
TCGTGTGCTAGGTTTAGGTG+AGG - contig337end:3489-3508 MsG0080048497.01.T01:intron 50.0%
TGAGGGTAGAGAGAGAACGA+CGG - contig337end:3431-3450 MsG0080048497.01.T01:intron 50.0%
TGCGTGCCAAATCCAAGTTC+TGG - contig337end:2016-2035 MsG0080048497.01.T01:three_prime_UTR 50.0%
TGGCTTCACGTCATAGAGTC+AGG - contig337end:4766-4785 MsG0080048497.01.T01:intron 50.0%
TGTCTTGAGCTTCCTGGTTG+GGG + contig337end:4913-4932 MsG0080048497.01.T01:intergenic 50.0%
TTTGGCACGCACTTCATTGG+TGG + contig337end:2007-2026 MsG0080048497.01.T01:intergenic 50.0%
! CGAGTTGGGCCTGTCAAAAT+TGG + contig337end:3603-3622 MsG0080048497.01.T01:intergenic 50.0%
! CGGTGGCCATGAATCTTTTC+CGG + contig337end:3468-3487 MsG0080048497.01.T01:intergenic 50.0%
! GCAAAGGTTAGCTGGGATTG+TGG + contig337end:4820-4839 MsG0080048497.01.T01:intergenic 50.0%
! TCTTTTGACCCTACCCGGTT+CGG - contig337end:3567-3586 MsG0080048497.01.T01:intron 50.0%
! TGGTGCTCTCCCTTTTGCAA+AGG + contig337end:4836-4855 MsG0080048497.01.T01:intergenic 50.0%
AACGACGGCGAGAAATTCGC+CGG - contig337end:3446-3465 MsG0080048497.01.T01:intron 55.0%
AACGGACGATCCTACGTGCT+GGG - contig337end:3727-3746 MsG0080048497.01.T01:intron 55.0%
AGAAGATTAGGCCCCCAACC+AGG - contig337end:4898-4917 MsG0080048497.01.T01:intron 55.0%
CCTACGTAGGTTCGATTGGC+CGG - contig337end:2965-2984 MsG0080048497.01.T01:intron 55.0%
CTCTTCCCACAACCTGGTAC+TGG + contig337end:1930-1949 MsG0080048497.01.T01:intergenic 55.0%
CTTCGATACCAGAGCCGAAC+CGG - contig337end:4672-4691 MsG0080048497.01.T01:CDS 55.0%
GAAGAGCTCTTCCCACAACC+TGG + contig337end:1936-1955 MsG0080048497.01.T01:intergenic 55.0%
GCAGGTGCTTGCCATCAATG+AGG - contig337end:2409-2428 MsG0080048497.01.T01:CDS 55.0%
GCATGTTAGGACTAGACGGC+TGG - contig337end:4084-4103 MsG0080048497.01.T01:intron 55.0%
GCCGTCTAGTCCTAACATGC+TGG + contig337end:4084-4103 MsG0080048497.01.T01:intergenic 55.0%
GTCGAGGCATGATTGGGTGT+GGG - contig337end:3693-3712 MsG0080048497.01.T01:intron 55.0%
GTCTTGAGCTTCCTGGTTGG+GGG + contig337end:4912-4931 MsG0080048497.01.T01:intergenic 55.0%
! ATTGGGTGTGGGAAGCACGT+AGG - contig337end:3704-3723 MsG0080048497.01.T01:intron 55.0%
! CTTTTGACCCTACCCGGTTC+GGG - contig337end:3568-3587 MsG0080048497.01.T01:intron 55.0%
! GTGTGGGAAGCACGTAGGAA+CGG - contig337end:3709-3728 MsG0080048497.01.T01:intron 55.0%
! TAGGTTCGATTGGCCGGTGT+AGG - contig337end:2971-2990 MsG0080048497.01.T01:intron 55.0%
!! TGTGATATCCGGTTCGGCTC+TGG + contig337end:4683-4702 MsG0080048497.01.T01:intergenic 55.0%
!! TTGGTTTGGGCTACCCGAAC+CGG + contig337end:3584-3603 MsG0080048497.01.T01:intergenic 55.0%
ATGGCCACCGTTCGTGTGCT+AGG - contig337end:3478-3497 MsG0080048497.01.T01:intron 60.0%
CCGGCCAATCGAACCTACGT+AGG + contig337end:2968-2987 MsG0080048497.01.T01:intergenic 60.0%
GAACGGACGATCCTACGTGC+TGG - contig337end:3726-3745 MsG0080048497.01.T01:intron 60.0%
GGTCGAGGCATGATTGGGTG+TGG - contig337end:3692-3711 MsG0080048497.01.T01:intron 60.0%
! TGGTTTGGGCTACCCGAACC+GGG + contig337end:3583-3602 MsG0080048497.01.T01:intergenic 60.0%
AGGCACGAGTCCCAGCACGT+AGG + contig337end:3740-3759 MsG0080048497.01.T01:intergenic 65.0%
TGGGCTACCCGAACCGGGTA+GGG + contig337end:3578-3597 MsG0080048497.01.T01:intergenic 65.0%
TTGGGCTACCCGAACCGGGT+AGG + contig337end:3579-3598 MsG0080048497.01.T01:intergenic 65.0%
Chromosome Type Strat End Strand Name
contig337end gene 1913 5160 1913 ID=MsG0080048497.01;Name=MsG0080048497.01
contig337end mRNA 1913 5160 1913 ID=MsG0080048497.01.T01;Parent=MsG0080048497.01;Name=MsG0080048497.01.T01;_AED=0.40;_eAED=0.40;_QI=0|1|0.66|1|1|1|3|192|176
contig337end exon 1913 2446 1913 ID=MsG0080048497.01.T01:exon:32798;Parent=MsG0080048497.01.T01
contig337end exon 4643 4706 4643 ID=MsG0080048497.01.T01:exon:32797;Parent=MsG0080048497.01.T01
contig337end exon 5036 5160 5036 ID=MsG0080048497.01.T01:exon:32796;Parent=MsG0080048497.01.T01
contig337end CDS 5036 5160 5036 ID=MsG0080048497.01.T01:cds;Parent=MsG0080048497.01.T01
contig337end CDS 4643 4706 4643 ID=MsG0080048497.01.T01:cds;Parent=MsG0080048497.01.T01
contig337end CDS 2105 2446 2105 ID=MsG0080048497.01.T01:cds;Parent=MsG0080048497.01.T01
contig337end three_prime_UTR 1913 2104 1913 ID=MsG0080048497.01.T01:three_prime_utr;Parent=MsG0080048497.01.T01
Gene Sequence

>MsG0080048497.01.T01

ATGAATCAGTATCACCAGTACCAGGTTGTGGGAAGAGCTCTTCCTACTGAGAAGGATGAACATCCTAAGATCTATAGGATGAAGTTGTGGGCCACCAATGAAGTGCGTGCCAAATCCAAGTTCTGGTATTTTTTGAGAAAGCTGAAGAAGGTTAAGAAGAGCAATGGGCAGGTGCTTGCCATCAATGAGATTTTTGAGAAAAATCCAACCAAGATTAAGAACTACGGAATTTGGCTTCGATACCAGAGCCGAACCGGATATCACAACATGTACAAGGAATACCGCGACACTACACTAAATGGTGCTGTTGAATATATGTACAATGAAATGGCTTCACGTCATAGAGTCAGGCATCCATGCATCCAGATCATCAAGACTGCCACAATCCCAGCTAACCTTTGCAAAAGGGAGAGCACCAAGCAGTTCCATAACTCGAAAATCAAGTTTCCTTTGGTGTTCAAGAAGATTAGGCCCCCAACCAGGAAGCTCAAGACAACATACAAGGCAACCAAACCCAACATGTTTATGTAA
Protein sequence

>MsG0080048497.01.T01

MNQYHQYQVVGRALPTEKDEHPKIYRMKLWATNEVRAKSKFWYFLRKLKKVKKSNGQVLAINEIFEKNPTKIKNYGIWLRYQSRTGYHNMYKEYRDTTLNGAVEYMYNEMASRHRVRHPCIQIIKTATIPANLCKRESTKQFHNSKIKFPLVFKKIRPPTRKLKTTYKATKPNMFM*