AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048621.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048621.01.T01 MTR_7g094600 80.198 202 8 1 1 202 1 170 4.17e-115 325
MsG0080048621.01.T01 MTR_1g072570 71.429 161 45 1 1 160 1 161 2.32e-87 255
MsG0080048621.01.T01 MTR_7g094600 76.582 158 5 1 45 202 7 132 8.48e-83 242
MsG0080048621.01.T01 MTR_8g098410 68.000 150 48 0 11 160 9 158 1.03e-76 228
MsG0080048621.01.T01 MTR_8g098410 68.000 150 48 0 11 160 75 224 8.25e-76 228
MsG0080048621.01.T01 MTR_1g014210 61.783 157 60 0 4 160 71 227 9.92e-73 220
MsG0080048621.01.T01 MTR_8g105630 60.784 153 60 0 8 160 5 157 8.22e-70 210
MsG0080048621.01.T01 MTR_8g098400 59.756 164 66 0 4 167 6 169 1.45e-69 210
MsG0080048621.01.T01 MTR_8g098400 60.510 157 62 0 4 160 6 162 1.68e-68 207
MsG0080048621.01.T01 MTR_5g010340 55.307 179 76 4 9 186 9 184 1.34e-66 203
MsG0080048621.01.T01 MTR_8g098410 70.526 95 28 0 11 105 75 169 9.36e-47 153
MsG0080048621.01.T01 MTR_8g098400 60.526 114 45 0 47 160 2 115 1.05e-46 150
MsG0080048621.01.T01 MTR_8g098400 58.824 102 42 0 4 105 6 107 1.40e-39 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048621.01.T01 AT2G48150 69.375 160 49 0 1 160 1 160 1.48e-83 245
MsG0080048621.01.T01 AT3G63080 71.141 149 43 0 12 160 14 162 5.66e-81 239
MsG0080048621.01.T01 AT4G11600 67.333 150 49 0 11 160 72 221 4.80e-75 226
MsG0080048621.01.T01 AT4G31870 63.057 157 58 0 4 160 68 224 2.03e-74 224
MsG0080048621.01.T01 AT2G25080 61.783 157 60 0 4 160 71 227 1.79e-73 222
MsG0080048621.01.T01 AT2G31570 64.706 153 54 0 8 160 5 157 1.11e-71 215
MsG0080048621.01.T01 AT1G63460 62.000 150 57 0 11 160 8 157 4.14e-70 211
MsG0080048621.01.T01 AT2G48150 70.149 134 40 0 27 160 1 134 5.96e-69 207
MsG0080048621.01.T01 AT2G43350 57.516 153 65 0 8 160 44 196 2.57e-64 197
MsG0080048621.01.T01 AT2G43350 57.516 153 65 0 8 160 44 196 2.57e-64 197
MsG0080048621.01.T01 AT4G31870 46.970 66 35 0 4 69 68 133 1.09e-18 80.1

Find 64 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAGCCAGCTTTAATAATA+TGG 0.167484 contig374end:-102777 MsG0080048621.01.T01:CDS
AAACAAAATGAATGTATATT+TGG 0.181906 contig374end:-102108 MsG0080048621.01.T01:three_prime_UTR
TGCTGTAAAGCTCAGTTAAC+TGG 0.245625 contig374end:+104963 None:intergenic
CTACCTTAACTGTGAATTCA+TGG 0.277645 contig374end:+105409 None:intergenic
TGCCTATGCTAAGATTGATT+TGG 0.289422 contig374end:+105561 None:intergenic
TTCCTTAAAGAAAAGAAATC+TGG 0.331564 contig374end:-102971 MsG0080048621.01.T01:CDS
GAAGCACCCATTTCTTTGTT+TGG 0.332386 contig374end:+105456 None:intergenic
CGCATTACCTTTCCGAAAAT+CGG 0.338888 contig374end:+104657 None:intergenic
GATATTACTTCCATGGAAAA+TGG 0.340924 contig374end:+102857 None:intergenic
GATTTCAGGACTTGAGATAT+TGG 0.343793 contig374end:-104769 MsG0080048621.01.T01:intron
GTTGTATCAAAATTCTCCTT+TGG 0.354184 contig374end:-102019 MsG0080048621.01.T01:three_prime_UTR
CTTTCCCTTTGTAAGTGCTT+AGG 0.358344 contig374end:+105255 None:intergenic
TGATTGCTGTGTATTACATT+TGG 0.371835 contig374end:-101951 MsG0080048621.01.T01:three_prime_UTR
TATTGAATATGTTATGTCTA+GGG 0.379333 contig374end:-102061 MsG0080048621.01.T01:three_prime_UTR
GCCTATGCTAAGATTGATTT+GGG 0.381471 contig374end:+105562 None:intergenic
TCTTTAAGGAATTTGTAGAT+AGG 0.397898 contig374end:+102983 None:intergenic
TACAGCAGATACAAGGATAA+AGG 0.414694 contig374end:-104947 MsG0080048621.01.T01:intron
TAGTTAACGTTGCTTCGAAA+TGG 0.425214 contig374end:-105223 MsG0080048621.01.T01:intron
AGAAATGGGTGCTTCAAATT+CGG 0.428500 contig374end:-105448 MsG0080048621.01.T01:exon
AATCCATGAATTCACAGTTA+AGG 0.431186 contig374end:-105412 MsG0080048621.01.T01:intron
TTATTGAATATGTTATGTCT+AGG 0.431966 contig374end:-102062 MsG0080048621.01.T01:three_prime_UTR
CAATCACATCAAATCATTGC+GGG 0.437674 contig374end:-102147 MsG0080048621.01.T01:three_prime_UTR
TATTTGCAGATACGTGTGAA+TGG 0.466900 contig374end:-103019 MsG0080048621.01.T01:intron
TTTGTAGATAGGTGCTGTGT+CGG 0.467391 contig374end:+102994 None:intergenic
CTGATATGTCAAAATTAGAA+TGG 0.469654 contig374end:+102795 None:intergenic
TCAAGAGCCGGGGAATAGTC+AGG 0.479762 contig374end:-104721 MsG0080048621.01.T01:CDS
AAAACACCAAACAAAGAAAT+GGG 0.487396 contig374end:-105462 MsG0080048621.01.T01:exon
TCAGCCAGCTTTAATAATAT+GGG 0.487579 contig374end:-102776 MsG0080048621.01.T01:CDS
CTCGGCTTTGAATCTTGTAC+AGG 0.488818 contig374end:+104683 None:intergenic
AATGACATTAAGAAGGCATT+GGG 0.490705 contig374end:-102189 MsG0080048621.01.T01:CDS
TGCTGTGTATTACATTTGGC+TGG 0.493065 contig374end:-101947 MsG0080048621.01.T01:three_prime_UTR
TTTGTAGAATGACATTAAGA+AGG 0.496225 contig374end:-102196 MsG0080048621.01.T01:intron
GGGGCCCATATTATTAAAGC+TGG 0.502224 contig374end:+102772 None:intergenic
TTCTAGGGTCTAGGATAAAG+TGG 0.507102 contig374end:-102946 MsG0080048621.01.T01:CDS
GAATGACATTAAGAAGGCAT+TGG 0.511513 contig374end:-102190 MsG0080048621.01.T01:CDS
CATTAAGAAGGCATTGGGAG+AGG 0.512162 contig374end:-102184 MsG0080048621.01.T01:CDS
ATGTAATACACAGCAATCAC+AGG 0.512859 contig374end:+101955 None:intergenic
CTTTCCGAAAATCGGATACT+CGG 0.513833 contig374end:+104665 None:intergenic
CAGTTTCTGAATCAAGAGCC+GGG 0.514222 contig374end:-104732 MsG0080048621.01.T01:CDS
GGCATTGGGAGAGGCTTGAA+AGG 0.516902 contig374end:-102175 MsG0080048621.01.T01:exon
TTGTAGATAGGTGCTGTGTC+GGG 0.532272 contig374end:+102995 None:intergenic
TCAGTTTCTGAATCAAGAGC+CGG 0.532928 contig374end:-104733 MsG0080048621.01.T01:CDS
AGAGGGAGATATTACTTCCA+TGG 0.535646 contig374end:+102850 None:intergenic
ATTGAATATGTTATGTCTAG+GGG 0.541174 contig374end:-102060 MsG0080048621.01.T01:three_prime_UTR
AGGTGGTTGGACTATAACGC+TGG 0.546220 contig374end:+102882 None:intergenic
TGAACCTAAGCACTTACAAA+GGG 0.559862 contig374end:-105259 MsG0080048621.01.T01:CDS
AAATAATGAATTATATCACA+AGG 0.577120 contig374end:+101996 None:intergenic
TGATTCAGAAACTGATTGCA+CGG 0.578787 contig374end:+104741 None:intergenic
GCTGTAAAGCTCAGTTAACT+GGG 0.578819 contig374end:+104964 None:intergenic
AGACATGGAGAGAAATAGAG+AGG 0.584927 contig374end:+102832 None:intergenic
ATGGAAAATGGTGAGGTGGT+TGG 0.585195 contig374end:+102869 None:intergenic
GACATGGAGAGAAATAGAGA+GGG 0.589935 contig374end:+102833 None:intergenic
TGAGCTTTACAGCAGATACA+AGG 0.602591 contig374end:-104954 MsG0080048621.01.T01:CDS
GTGAACCTAAGCACTTACAA+AGG 0.611486 contig374end:-105260 MsG0080048621.01.T01:CDS
TCTGCCTCCTGACTATTCCC+CGG 0.618651 contig374end:+104714 None:intergenic
ACCCAAATCAATCTTAGCAT+AGG 0.621397 contig374end:-105563 MsG0080048621.01.T01:five_prime_UTR
TACTTCCATGGAAAATGGTG+AGG 0.621803 contig374end:+102862 None:intergenic
GAATTATATCACAAGGCCAA+AGG 0.625003 contig374end:+102003 None:intergenic
TTCCATGGAAAATGGTGAGG+TGG 0.643848 contig374end:+102865 None:intergenic
ACAATCACATCAAATCATTG+CGG 0.649434 contig374end:-102148 MsG0080048621.01.T01:three_prime_UTR
GAATGAACATAACAGAGACA+TGG 0.652591 contig374end:+102817 None:intergenic
AGTTTCTGAATCAAGAGCCG+GGG 0.680134 contig374end:-104731 MsG0080048621.01.T01:CDS
TGTAATACACAGCAATCACA+GGG 0.683418 contig374end:+101956 None:intergenic
AGAGCCGGGGAATAGTCAGG+AGG 0.703784 contig374end:-104718 MsG0080048621.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGTTGTAAAAAAATAAA+AGG - contig374end:105114-105133 MsG0080048621.01.T01:intron 10.0%
!! ATTGATTAAAAACATAAATT+AGG - contig374end:103312-103331 MsG0080048621.01.T01:intron 10.0%
!!! GATTAAATTAGTTTTTAATA+GGG - contig374end:102439-102458 MsG0080048621.01.T01:intron 10.0%
!!! GTTAATTTTTTCTTATAAAT+AGG - contig374end:103270-103289 MsG0080048621.01.T01:intron 10.0%
!!! TGATTAAATTAGTTTTTAAT+AGG - contig374end:102438-102457 MsG0080048621.01.T01:intron 10.0%
!! AAACAAAATGAATGTATATT+TGG - contig374end:105377-105396 MsG0080048621.01.T01:intron 15.0%
!! AAATAATGAATTATATCACA+AGG + contig374end:105492-105511 None:intergenic 15.0%
!! CAAAGAAAAAATAAAACAAA+AGG + contig374end:104097-104116 None:intergenic 15.0%
!! TAATAAACATGATAGAATAT+AGG + contig374end:105156-105175 None:intergenic 15.0%
!!! AATATTTTTTTGAAGTAAAC+AGG + contig374end:104909-104928 None:intergenic 15.0%
!!! ATTTTAAGTTTTACTAACAA+GGG - contig374end:104959-104978 MsG0080048621.01.T01:CDS 15.0%
!!! TCTTTTCTTTTAATTCATTT+TGG + contig374end:102938-102957 None:intergenic 15.0%
!!! TTTAGCTAATTTTGTTTATT+GGG - contig374end:102462-102481 MsG0080048621.01.T01:intron 15.0%
!!! TTTTCATAAAACAAAATTGT+TGG - contig374end:105242-105261 MsG0080048621.01.T01:CDS 15.0%
!!! TTTTGCTAGTTTTTTTAATT+GGG - contig374end:102408-102427 MsG0080048621.01.T01:intron 15.0%
!!! TTTTTTGTAAAGAGTTAAAA+GGG + contig374end:102121-102140 None:intergenic 15.0%
!!! TTTTTTTGTAAAGAGTTAAA+AGG + contig374end:102122-102141 None:intergenic 15.0%
!! AAAAAATAGAAGGAAAAACT+AGG - contig374end:105018-105037 MsG0080048621.01.T01:intron 20.0%
!! AAAAATTGAATTAGAGAAAG+TGG + contig374end:102295-102314 None:intergenic 20.0%
!! AAAATTGAATTAGAGAAAGT+GGG + contig374end:102294-102313 None:intergenic 20.0%
!! AATAACGAATCAAATATATC+TGG + contig374end:104447-104466 None:intergenic 20.0%
!! ACATAACAAGTTTAATAGAA+GGG + contig374end:103085-103104 None:intergenic 20.0%
!! AGCAAAGTAACAAAAAAAAA+AGG + contig374end:102146-102165 None:intergenic 20.0%
!! ATGATAATCATATAAACGTT+GGG + contig374end:104736-104755 None:intergenic 20.0%
!! CATTGCATAAATAATGTATT+TGG - contig374end:103201-103220 MsG0080048621.01.T01:intron 20.0%
!! GAAAATAGAAAACAAGAAAA+GGG - contig374end:105184-105203 MsG0080048621.01.T01:intron 20.0%
!! GCAAAGTAACAAAAAAAAAA+GGG + contig374end:102145-102164 None:intergenic 20.0%
!! GTCAAATGTTTCTTATAAAT+AGG + contig374end:103171-103190 None:intergenic 20.0%
!! GTTGTAATTATGTTGTTAAT+CGG - contig374end:102979-102998 MsG0080048621.01.T01:CDS 20.0%
!! TAAAGATGTCAATATATCAA+TGG - contig374end:104803-104822 MsG0080048621.01.T01:intron 20.0%
!! TATTGAATATGTTATGTCTA+GGG - contig374end:105424-105443 MsG0080048621.01.T01:CDS 20.0%
!! TGAAAATAGAAAACAAGAAA+AGG - contig374end:105183-105202 MsG0080048621.01.T01:intron 20.0%
!! TTAATCATAATATGACAACT+AGG - contig374end:103533-103552 MsG0080048621.01.T01:intron 20.0%
!! TTATGGTTTAATTGTTACTA+GGG - contig374end:102354-102373 MsG0080048621.01.T01:intron 20.0%
!! TTATTGAATATGTTATGTCT+AGG - contig374end:105423-105442 MsG0080048621.01.T01:CDS 20.0%
!! TTGTCATATTATGATTAACT+AGG + contig374end:103531-103550 None:intergenic 20.0%
!! TTGTGATAGAAAAAAATAGA+AGG - contig374end:105008-105027 MsG0080048621.01.T01:intron 20.0%
!! TTTATGGTTTAATTGTTACT+AGG - contig374end:102353-102372 MsG0080048621.01.T01:intron 20.0%
!!! AAAAGAGTACAATGATTTTT+TGG - contig374end:102952-102971 MsG0080048621.01.T01:CDS 20.0%
!!! CATTTTAAGTTTTACTAACA+AGG - contig374end:104958-104977 MsG0080048621.01.T01:CDS 20.0%
!!! GATAAAGGTATGATTTTTAA+GGG - contig374end:102553-102572 MsG0080048621.01.T01:intron 20.0%
!!! GTTTAGCTAATTTTGTTTAT+TGG - contig374end:102461-102480 MsG0080048621.01.T01:intron 20.0%
!!! GTTTTGCTAGTTTTTTTAAT+TGG - contig374end:102407-102426 MsG0080048621.01.T01:intron 20.0%
!!! TTTAGCCTTAATAAATGATA+GGG + contig374end:104787-104806 None:intergenic 20.0%
!!! TTTCTGTTTTGCTTAATATA+GGG + contig374end:101965-101984 None:intergenic 20.0%
!!! TTTGCTAGTTTTTTTAATTG+GGG - contig374end:102409-102428 MsG0080048621.01.T01:intron 20.0%
!!! TTTTCTGTTTTGCTTAATAT+AGG + contig374end:101966-101985 None:intergenic 20.0%
!!! TTTTTTCTTATAAATAGGAC+CGG - contig374end:103275-103294 MsG0080048621.01.T01:intron 20.0%
! AAAAACACCAAACAAAGAAA+TGG - contig374end:102022-102041 MsG0080048621.01.T01:three_prime_UTR 25.0%
! AAAACACCAAACAAAGAAAT+GGG - contig374end:102023-102042 MsG0080048621.01.T01:three_prime_UTR 25.0%
! AAAGATGAATAAACATTCAG+AGG + contig374end:104340-104359 None:intergenic 25.0%
! AAATTGAATTAGAGAAAGTG+GGG + contig374end:102293-102312 None:intergenic 25.0%
! AGTTCAATTTCCAATAGAAT+TGG + contig374end:104060-104079 None:intergenic 25.0%
! ATAAACATGTGAGTAAAGTA+AGG + contig374end:103994-104013 None:intergenic 25.0%
! ATGTTTCTTATAAATAGGAC+CGG + contig374end:103166-103185 None:intergenic 25.0%
! ATTGAATATGTTATGTCTAG+GGG - contig374end:105425-105444 MsG0080048621.01.T01:CDS 25.0%
! CATCCTGTAAGAAAAAAAAA+GGG + contig374end:102191-102210 None:intergenic 25.0%
! CATCTTTACAAATGATGATT+AGG - contig374end:104353-104372 MsG0080048621.01.T01:intron 25.0%
! CTGATATGTCAAAATTAGAA+TGG + contig374end:104693-104712 None:intergenic 25.0%
! CTGTAAATTATAGAATCCAT+TGG - contig374end:103912-103931 MsG0080048621.01.T01:intron 25.0%
! GAAATTGAACTTGAAAAAAC+TGG - contig374end:104069-104088 MsG0080048621.01.T01:intron 25.0%
! GACATAACAAGTTTAATAGA+AGG + contig374end:103086-103105 None:intergenic 25.0%
! GATGATAATCATATAAACGT+TGG + contig374end:104737-104756 None:intergenic 25.0%
! TATATCTGGTTAACAAACAA+TGG + contig374end:104433-104452 None:intergenic 25.0%
! TGATAATCATATAAACGTTG+GGG + contig374end:104735-104754 None:intergenic 25.0%
! TTCCTTAAAGAAAAGAAATC+TGG - contig374end:104514-104533 MsG0080048621.01.T01:intron 25.0%
! TTCTTAATGTCATTCTACAA+AGG + contig374end:105290-105309 None:intergenic 25.0%
! TTTGTAGAATGACATTAAGA+AGG - contig374end:105289-105308 MsG0080048621.01.T01:intron 25.0%
!! AACCAGATTTCTTTTCTTTA+AGG + contig374end:104519-104538 None:intergenic 25.0%
!! GAAAGCAGTTTAAGAAATTT+TGG + contig374end:105094-105113 None:intergenic 25.0%
!! TCTTTAAGGAATTTGTAGAT+AGG + contig374end:104505-104524 None:intergenic 25.0%
!! TTCTGTTTTGCTTAATATAG+GGG + contig374end:101964-101983 None:intergenic 25.0%
!!! AAAAGAAATCTGGTTTTCTA+GGG - contig374end:104524-104543 MsG0080048621.01.T01:intron 25.0%
!!! AAACCCTTTTTTTTTCTTAC+AGG - contig374end:102185-102204 MsG0080048621.01.T01:CDS 25.0%
!!! AACCTAATTTTAATTCGATG+AGG + contig374end:103869-103888 None:intergenic 25.0%
!!! AATTTTAAGTTGGTCTACAA+CGG - contig374end:103008-103027 MsG0080048621.01.T01:CDS 25.0%
!!! ACCTAATTTTAATTCGATGA+GGG + contig374end:103868-103887 None:intergenic 25.0%
!!! ATTCAGGTCAATTTTTGAAA+GGG - contig374end:103439-103458 MsG0080048621.01.T01:intron 25.0%
!!! CTTTAGCCTTAATAAATGAT+AGG + contig374end:104788-104807 None:intergenic 25.0%
!!! GGATAAAGGTATGATTTTTA+AGG - contig374end:102552-102571 MsG0080048621.01.T01:intron 25.0%
!!! TATTGGGTTTTTAATTACAG+TGG - contig374end:102478-102497 MsG0080048621.01.T01:intron 25.0%
!!! TCGGTTAAGTAATTTTAAGT+TGG - contig374end:102998-103017 MsG0080048621.01.T01:CDS 25.0%
!!! TTAACTAGGAGATTTGATTT+TGG + contig374end:103517-103536 None:intergenic 25.0%
!!! TTTCTTTGTTTGGTGTTTTT+TGG + contig374end:102022-102041 None:intergenic 25.0%
AAAAGGGTTTGAGAAAAAGT+AGG + contig374end:102105-102124 None:intergenic 30.0%
AATCCATGAATTCACAGTTA+AGG - contig374end:102073-102092 MsG0080048621.01.T01:three_prime_UTR 30.0%
AATTTCCAATAGAATTGGAC+AGG + contig374end:104055-104074 None:intergenic 30.0%
ACAATCACATCAAATCATTG+CGG - contig374end:105337-105356 MsG0080048621.01.T01:intron 30.0%
CAGTCTAAGTAACTTCAAAA+TGG + contig374end:102643-102662 None:intergenic 30.0%
CCAATCTTTCTTTCTCTTTA+TGG - contig374end:102337-102356 MsG0080048621.01.T01:intron 30.0%
CCATAAAGAGAAAGAAAGAT+TGG + contig374end:102340-102359 None:intergenic 30.0%
CGAAGAGATACAATAATTTC+TGG + contig374end:103792-103811 None:intergenic 30.0%
GATATTACTTCCATGGAAAA+TGG + contig374end:104631-104650 None:intergenic 30.0%
GCATCCTGTAAGAAAAAAAA+AGG + contig374end:102192-102211 None:intergenic 30.0%
GGTAAATCCTATATGGTATT+CGG + contig374end:103114-103133 None:intergenic 30.0%
GTTGTATCAAAATTCTCCTT+TGG - contig374end:105466-105485 MsG0080048621.01.T01:exon 30.0%
TACATTGCTTCATAAGTACA+TGG + contig374end:103950-103969 None:intergenic 30.0%
TCTCAACCTAATAAGATGTA+AGG - contig374end:104755-104774 MsG0080048621.01.T01:CDS 30.0%
TGATTGCTGTGTATTACATT+TGG - contig374end:105534-105553 MsG0080048621.01.T01:five_prime_UTR 30.0%
TTATTGAGTGAAATCTCATG+TGG - contig374end:103630-103649 MsG0080048621.01.T01:intron 30.0%
TTGAAGTTACTTAGACTGAA+GGG - contig374end:102645-102664 MsG0080048621.01.T01:intron 30.0%
! ATAATCTCACATGGTTTTCT+AGG - contig374end:104833-104852 MsG0080048621.01.T01:intron 30.0%
! ATCAGCCAGCTTTAATAATA+TGG - contig374end:104708-104727 MsG0080048621.01.T01:CDS 30.0%
! GAAAAAGTACAAACACAGAA+AGG + contig374end:103469-103488 None:intergenic 30.0%
! GATTCAGGTCAATTTTTGAA+AGG - contig374end:103438-103457 MsG0080048621.01.T01:intron 30.0%
! GCTAGCATTTTTCATCTTTT+TGG + contig374end:103576-103595 None:intergenic 30.0%
! TCAGCCAGCTTTAATAATAT+GGG - contig374end:104709-104728 MsG0080048621.01.T01:CDS 30.0%
! TTATACATGGACAACAGTTT+TGG + contig374end:103135-103154 None:intergenic 30.0%
! TTTGAAGTTACTTAGACTGA+AGG - contig374end:102644-102663 MsG0080048621.01.T01:intron 30.0%
! TTTTTCTTACAGGATGCAAA+GGG - contig374end:102195-102214 MsG0080048621.01.T01:intron 30.0%
!! AATGACATTAAGAAGGCATT+GGG - contig374end:105296-105315 MsG0080048621.01.T01:intron 30.0%
!! CAGTTTTGGTAAATCCTATA+TGG + contig374end:103121-103140 None:intergenic 30.0%
!! CTGCTGACATTTTTCTAAAA+AGG - contig374end:103721-103740 MsG0080048621.01.T01:intron 30.0%
!! TCAGTTGAGAATGTTTTTGT+TGG - contig374end:102691-102710 MsG0080048621.01.T01:intron 30.0%
!! TGTTTTTGTTGGTTGATTTC+AGG - contig374end:102702-102721 MsG0080048621.01.T01:intron 30.0%
!!! CCTAATTTTAATTCGATGAG+GGG + contig374end:103867-103886 None:intergenic 30.0%
!!! CTTTTTTCAATGACTTGACT+AGG - contig374end:103376-103395 MsG0080048621.01.T01:intron 30.0%
!!! GAAAAGAAATCTGGTTTTCT+AGG - contig374end:104523-104542 MsG0080048621.01.T01:intron 30.0%
!!! TTTTTTCTTACAGGATGCAA+AGG - contig374end:102194-102213 MsG0080048621.01.T01:intron 30.0%
!!! TTTTTTGGTTGTTGAAGATG+TGG + contig374end:102007-102026 None:intergenic 30.0%
AAAGAGATGCATGACTATCT+AGG + contig374end:104393-104412 None:intergenic 35.0%
AATCCACTCAAATGAGATTC+AGG - contig374end:103423-103442 MsG0080048621.01.T01:intron 35.0%
ACCCAAATCAATCTTAGCAT+AGG - contig374end:101922-101941 MsG0080048621.01.T01:three_prime_UTR 35.0%
ACCTAATAAGATGTAAGGAG+TGG - contig374end:104760-104779 MsG0080048621.01.T01:CDS 35.0%
AGAAATGGGTGCTTCAAATT+CGG - contig374end:102037-102056 MsG0080048621.01.T01:three_prime_UTR 35.0%
ATAGAATTGGACAGGTCAAT+AGG + contig374end:104047-104066 None:intergenic 35.0%
ATATACAGGGAAATTTGTCG+TGG - contig374end:104283-104302 MsG0080048621.01.T01:intron 35.0%
ATGAATAAACATTCAGAGGC+AGG + contig374end:104336-104355 None:intergenic 35.0%
ATGGCAAATGTGAAACATGT+TGG - contig374end:105210-105229 MsG0080048621.01.T01:intron 35.0%
ATGTAATACACAGCAATCAC+AGG + contig374end:105533-105552 None:intergenic 35.0%
ATTATAGAATCCATTGGCGA+CGG - contig374end:103918-103937 MsG0080048621.01.T01:intron 35.0%
ATTGACCTGTCCAATTCTAT+TGG - contig374end:104047-104066 MsG0080048621.01.T01:intron 35.0%
CAATCACATCAAATCATTGC+GGG - contig374end:105338-105357 MsG0080048621.01.T01:intron 35.0%
CCCCTCATCGAATTAAAATT+AGG - contig374end:103864-103883 MsG0080048621.01.T01:intron 35.0%
CTACCTTAACTGTGAATTCA+TGG + contig374end:102079-102098 None:intergenic 35.0%
GAAAACAAGAAAAGGGAACA+TGG - contig374end:105191-105210 MsG0080048621.01.T01:intron 35.0%
GAAATCCAGCTAATAGATAG+TGG + contig374end:103672-103691 None:intergenic 35.0%
GAATGAACATAACAGAGACA+TGG + contig374end:104671-104690 None:intergenic 35.0%
GAATTATATCACAAGGCCAA+AGG + contig374end:105485-105504 None:intergenic 35.0%
GATTTCAGGACTTGAGATAT+TGG - contig374end:102716-102735 MsG0080048621.01.T01:intron 35.0%
GTGGACCCTATCATTTATTA+AGG - contig374end:104779-104798 MsG0080048621.01.T01:intron 35.0%
TACAGCAGATACAAGGATAA+AGG - contig374end:102538-102557 MsG0080048621.01.T01:intron 35.0%
TAGTTAACGTTGCTTCGAAA+TGG - contig374end:102262-102281 MsG0080048621.01.T01:intron 35.0%
TATTTGCAGATACGTGTGAA+TGG - contig374end:104466-104485 MsG0080048621.01.T01:intron 35.0%
TCACCGGAAAACAATAACTA+GGG + contig374end:103824-103843 None:intergenic 35.0%
TCCACTCCTTACATCTTATT+AGG + contig374end:104764-104783 None:intergenic 35.0%
TCTTTGATACTCTCTTCGTT+AGG + contig374end:103696-103715 None:intergenic 35.0%
TGAACCTAAGCACTTACAAA+GGG - contig374end:102226-102245 MsG0080048621.01.T01:intron 35.0%
TGAGCAACTATTCAATGTGT+TGG - contig374end:104251-104270 MsG0080048621.01.T01:intron 35.0%
TGATTCAGAAACTGATTGCA+CGG + contig374end:102747-102766 None:intergenic 35.0%
TGCCTATGCTAAGATTGATT+TGG + contig374end:101927-101946 None:intergenic 35.0%
TGTAATACACAGCAATCACA+GGG + contig374end:105532-105551 None:intergenic 35.0%
TTCAACAATAAGCTCAATCC+AGG + contig374end:102904-102923 None:intergenic 35.0%
TTCCTTGTCAACCAAAAACT+TGG + contig374end:104572-104591 None:intergenic 35.0%
TTCTTATAAATAGGACCGGA+GGG + contig374end:103298-103279 None:intergenic 35.0%
TTGGTAGACGGAAATATACA+GGG - contig374end:104270-104289 MsG0080048621.01.T01:intron 35.0%
TTTCTTATAAATAGGACCGG+AGG + contig374end:103297-103278 None:intergenic 35.0%
! ACATGATAGTTTTTCTGTCC+TGG - contig374end:102883-102902 MsG0080048621.01.T01:CDS 35.0%
! AGACCCTAGTTATTGTTTTC+CGG - contig374end:103818-103837 MsG0080048621.01.T01:intron 35.0%
! CTATTCAATGTGTTGGTAGA+CGG - contig374end:104258-104277 MsG0080048621.01.T01:intron 35.0%
! GAATGACATTAAGAAGGCAT+TGG - contig374end:105295-105314 MsG0080048621.01.T01:intron 35.0%
! GCCTATGCTAAGATTGATTT+GGG + contig374end:101926-101945 None:intergenic 35.0%
! GTGGAATTTCACCAAGTTTT+TGG - contig374end:104558-104577 MsG0080048621.01.T01:intron 35.0%
!! AGATAGTGGACATCACTAAT+AGG + contig374end:103658-103677 None:intergenic 35.0%
!! GATAGTGGACATCACTAATA+GGG + contig374end:103657-103676 None:intergenic 35.0%
!!! AAGTTTTTGGTTGACAAGGA+AGG - contig374end:104571-104590 MsG0080048621.01.T01:intron 35.0%
!!! AGTTTTTGGTTGACAAGGAA+GGG - contig374end:104572-104591 MsG0080048621.01.T01:intron 35.0%
!!! GAAGTGCTGATTTTTGCATT+TGG - contig374end:102579-102598 MsG0080048621.01.T01:intron 35.0%
!!! TTATGTCTAGGGGTTTTGTT+TGG - contig374end:105435-105454 MsG0080048621.01.T01:CDS 35.0%
AACGGTCGTAATCTTTAAGC+GGG - contig374end:103026-103045 MsG0080048621.01.T01:intron 40.0%
AGACATGGAGAGAAATAGAG+AGG + contig374end:104656-104675 None:intergenic 40.0%
AGAGGGAGATATTACTTCCA+TGG + contig374end:104638-104657 None:intergenic 40.0%
ATACAAGCAAGTCAACTTGC+AGG + contig374end:104022-104041 None:intergenic 40.0%
CAACGGTCGTAATCTTTAAG+CGG - contig374end:103025-103044 MsG0080048621.01.T01:intron 40.0%
CGCATTACCTTTCCGAAAAT+CGG + contig374end:102831-102850 None:intergenic 40.0%
CTTACACAATAACGAGTCAC+CGG + contig374end:103840-103859 None:intergenic 40.0%
CTTTCCCTTTGTAAGTGCTT+AGG + contig374end:102233-102252 None:intergenic 40.0%
CTTTCCGAAAATCGGATACT+CGG + contig374end:102823-102842 None:intergenic 40.0%
GACATGGAGAGAAATAGAGA+GGG + contig374end:104655-104674 None:intergenic 40.0%
GATGTCCACTATCTATTAGC+TGG - contig374end:103664-103683 MsG0080048621.01.T01:intron 40.0%
GCTGTAAAGCTCAGTTAACT+GGG + contig374end:102524-102543 None:intergenic 40.0%
GGAAGATGTCATGTTGCATA+AGG - contig374end:103047-103066 MsG0080048621.01.T01:intron 40.0%
GTCACCGGAAAACAATAACT+AGG + contig374end:103825-103844 None:intergenic 40.0%
GTGAACCTAAGCACTTACAA+AGG - contig374end:102225-102244 MsG0080048621.01.T01:intron 40.0%
GTTGTAGACCAATCCAGTAA+TGG + contig374end:104162-104181 None:intergenic 40.0%
TACAAGCAAGTCAACTTGCA+GGG + contig374end:104021-104040 None:intergenic 40.0%
TACTTCCATGGAAAATGGTG+AGG + contig374end:104626-104645 None:intergenic 40.0%
TCAATGACTTGACTAGGTCT+AGG - contig374end:103382-103401 MsG0080048621.01.T01:intron 40.0%
TCAGTTTCTGAATCAAGAGC+CGG - contig374end:102752-102771 MsG0080048621.01.T01:intron 40.0%
TCATCGACCGAATACCATAT+AGG - contig374end:103104-103123 MsG0080048621.01.T01:intron 40.0%
TCCATGTATAATACTCCCTC+CGG - contig374end:103144-103163 MsG0080048621.01.T01:intron 40.0%
TGACCTGAATCTCATTTGAG+TGG + contig374end:103429-103448 None:intergenic 40.0%
TGAGCTTTACAGCAGATACA+AGG - contig374end:102531-102550 MsG0080048621.01.T01:intron 40.0%
TGCTGTAAAGCTCAGTTAAC+TGG + contig374end:102525-102544 None:intergenic 40.0%
TGCTGTGTATTACATTTGGC+TGG - contig374end:105538-105557 MsG0080048621.01.T01:five_prime_UTR 40.0%
TGGAAAAGTTCTGTGAGACA+AGG + contig374end:103772-103791 None:intergenic 40.0%
! AAAGCCGAGTATCCGATTTT+CGG - contig374end:102816-102835 MsG0080048621.01.T01:CDS 40.0%
! ATTTCACTGTGTGCCATTAC+TGG - contig374end:104146-104165 MsG0080048621.01.T01:intron 40.0%
! GAAGCACCCATTTCTTTGTT+TGG + contig374end:102032-102051 None:intergenic 40.0%
! GTTGGTAGACGGAAATATAC+AGG - contig374end:104269-104288 MsG0080048621.01.T01:intron 40.0%
! TTTGTAGATAGGTGCTGTGT+CGG + contig374end:104494-104513 None:intergenic 40.0%
!! TTCTAGGGTCTAGGATAAAG+TGG - contig374end:104539-104558 MsG0080048621.01.T01:intron 40.0%
!!! AATCTGGTTTTCTAGGGTCT+AGG - contig374end:104530-104549 MsG0080048621.01.T01:intron 40.0%
!!! CACCAAGTTTTTGGTTGACA+AGG - contig374end:104567-104586 MsG0080048621.01.T01:intron 40.0%
AAGGCGTGAAACACAGATGA+AGG + contig374end:103975-103994 None:intergenic 45.0%
AGTTTCTGAATCAAGAGCCG+GGG - contig374end:102754-102773 MsG0080048621.01.T01:intron 45.0%
ATGGAAAATGGTGAGGTGGT+TGG + contig374end:104619-104638 None:intergenic 45.0%
CAGTTTCTGAATCAAGAGCC+GGG - contig374end:102753-102772 MsG0080048621.01.T01:intron 45.0%
GGAGTGAGCATAATCTCACA+TGG - contig374end:104824-104843 MsG0080048621.01.T01:intron 45.0%
GGGGCCCATATTATTAAAGC+TGG + contig374end:104716-104735 None:intergenic 45.0%
GTTGCTCATTTGACAAGACG+CGG + contig374end:104239-104258 None:intergenic 45.0%
TAAATAGGACCGGAGAGAGT+AGG - contig374end:103285-103304 MsG0080048621.01.T01:intron 45.0%
TCAATACGACCTACTCTCTC+CGG + contig374end:103297-103316 None:intergenic 45.0%
TTCCATGGAAAATGGTGAGG+TGG + contig374end:104623-104642 None:intergenic 45.0%
! AACCACCTCACCATTTTCCA+TGG - contig374end:104618-104637 MsG0080048621.01.T01:intron 45.0%
! AATTTGTCGTGGTGTCAGCT+AGG - contig374end:104294-104313 MsG0080048621.01.T01:intron 45.0%
! ACTGTGTGCCATTACTGGAT+TGG - contig374end:104151-104170 MsG0080048621.01.T01:intron 45.0%
! CGAGTATCCGATTTTCGGAA+AGG - contig374end:102821-102840 MsG0080048621.01.T01:CDS 45.0%
! TTGTAGATAGGTGCTGTGTC+GGG + contig374end:104493-104512 None:intergenic 45.0%
!! ACCGGAGGGAGTATTATACA+TGG + contig374end:103148-103167 None:intergenic 45.0%
!! ACTGGATTGGTCTACAACTC+TGG - contig374end:104164-104183 MsG0080048621.01.T01:intron 45.0%
!! CATTAAGAAGGCATTGGGAG+AGG - contig374end:105301-105320 MsG0080048621.01.T01:intron 45.0%
!! CTCGGCTTTGAATCTTGTAC+AGG + contig374end:102805-102824 None:intergenic 45.0%
!! CTGGATTGGTCTACAACTCT+GGG - contig374end:104165-104184 MsG0080048621.01.T01:intron 45.0%
!! AATTTAATTAATTGTTTAAT+AGG - contig374end:102384-102403 MsG0080048621.01.T01:intron 5.0%
!! ATTTAATTAATTGTTTAATA+GGG - contig374end:102385-102404 MsG0080048621.01.T01:intron 5.0%
AGAATCCATTGGCGACGGAT+TGG - contig374end:103923-103942 MsG0080048621.01.T01:intron 50.0%
AGCAAGTCAACTTGCAGGGT+TGG + contig374end:104017-104036 None:intergenic 50.0%
AGGTGGTTGGACTATAACGC+TGG + contig374end:104606-104625 None:intergenic 50.0%
! GAATCCATTGGCGACGGATT+GGG - contig374end:103924-103943 MsG0080048621.01.T01:intron 50.0%
TCAAGAGCCGGGGAATAGTC+AGG - contig374end:102764-102783 MsG0080048621.01.T01:intron 55.0%
TCTGCCTCCTGACTATTCCC+CGG + contig374end:102774-102793 None:intergenic 55.0%
! GGCATTGGGAGAGGCTTGAA+AGG - contig374end:105310-105329 MsG0080048621.01.T01:intron 55.0%
AGAGCCGGGGAATAGTCAGG+AGG - contig374end:102767-102786 MsG0080048621.01.T01:intron 60.0%
ATGGCCCAATCCGTCGCCAA+TGG + contig374end:103931-103950 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
contig374end gene 101917 105590 101917 ID=MsG0080048621.01;Name=MsG0080048621.01
contig374end mRNA 101917 105590 101917 ID=MsG0080048621.01.T01;Parent=MsG0080048621.01;Name=MsG0080048621.01.T01;_AED=0.50;_eAED=0.53;_QI=124|0.8|0.83|1|0.6|0.5|6|262|202
contig374end exon 105413 105590 105413 ID=MsG0080048621.01.T01:exon:37834;Parent=MsG0080048621.01.T01
contig374end exon 105224 105300 105224 ID=MsG0080048621.01.T01:exon:37835;Parent=MsG0080048621.01.T01
contig374end exon 104948 105009 104948 ID=MsG0080048621.01.T01:exon:37836;Parent=MsG0080048621.01.T01
contig374end exon 104665 104783 104665 ID=MsG0080048621.01.T01:exon:37837;Parent=MsG0080048621.01.T01
contig374end exon 102769 103032 102769 ID=MsG0080048621.01.T01:exon:37838;Parent=MsG0080048621.01.T01
contig374end exon 101917 102211 101917 ID=MsG0080048621.01.T01:exon:37839;Parent=MsG0080048621.01.T01
contig374end five_prime_UTR 105467 105590 105467 ID=MsG0080048621.01.T01:five_prime_utr;Parent=MsG0080048621.01.T01
contig374end CDS 105413 105466 105413 ID=MsG0080048621.01.T01:cds;Parent=MsG0080048621.01.T01
contig374end CDS 105224 105300 105224 ID=MsG0080048621.01.T01:cds;Parent=MsG0080048621.01.T01
contig374end CDS 104948 105009 104948 ID=MsG0080048621.01.T01:cds;Parent=MsG0080048621.01.T01
contig374end CDS 104665 104783 104665 ID=MsG0080048621.01.T01:cds;Parent=MsG0080048621.01.T01
contig374end CDS 102769 103032 102769 ID=MsG0080048621.01.T01:cds;Parent=MsG0080048621.01.T01
contig374end CDS 102179 102211 102179 ID=MsG0080048621.01.T01:cds;Parent=MsG0080048621.01.T01
contig374end three_prime_UTR 101917 102178 101917 ID=MsG0080048621.01.T01:three_prime_utr;Parent=MsG0080048621.01.T01
Gene Sequence

>MsG0080048621.01.T01

ATGGGTGCTTCAAATTCGGTCTCTGAAAATTCAATCCATGAATTCACAGTTAAGGATGCAAAGGGAAGAGATGTGAACCTAAGCACTTACAAAGGGAAAGTGATTATTGTAGTTAACGTTGCTTCGAAATGTGGATTTACGAATGTCAATTATACCCAGTTAACTGAGCTTTACAGCAGATACAAGGATAAAGGACTTGAGATATTGGCTTTTCCGTGCAATCAGTTTCTGAATCAAGAGCCGGGGAATAGTCAGGAGGCAGAGCAATTTGCCTGTACAAGATTCAAAGCCGAGTATCCGATTTTCGGAAAGATACGTGTGAATGGACCCGACACAGCACCTATCTACAAATTCCTTAAAGAAAAGAAATCTGGTTTTCTAGGGTCTAGGATAAAGTGGAATTTCACCAAGTTTTTGGTTGACAAGGAAGGGCATGTGCTCCAGCGTTATAGTCCAACCACCTCACCATTTTCCATGGAAGTAATATCTCCCTCTCTATTTCTCTCCATGTCTCTGTTATGTTCATTCCATTCTAATTTTGACATATCAGCCAGCTTTAATAATATGGGCCCCAACAATGACATTAAGAAGGCATTGGGAGAGGCTTGA
Protein sequence

>MsG0080048621.01.T01

MGASNSVSENSIHEFTVKDAKGRDVNLSTYKGKVIIVVNVASKCGFTNVNYTQLTELYSRYKDKGLEILAFPCNQFLNQEPGNSQEAEQFACTRFKAEYPIFGKIRVNGPDTAPIYKFLKEKKSGFLGSRIKWNFTKFLVDKEGHVLQRYSPTTSPFSMEVISPSLFLSMSLLCSFHSNFDISASFNNMGPNNDIKKALGEA*