AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025670.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTGGGGTTGGTGGAGTT+GGG 0.211464 5:-22385805 MsG0580025670.01.T01:CDS
GCTGCAGGTGCTGGAGATTT+TGG 0.218185 5:-22385827 MsG0580025670.01.T01:CDS
GGTGCTGGGGTTGGTGGAGT+TGG 0.250635 5:-22385806 MsG0580025670.01.T01:CDS
GGCTGTGGGTGGAGTGGCTG+TGG 0.294604 5:-22385858 MsG0580025670.01.T01:CDS
TGCTGGGGTTGGTGGAGTTG+GGG 0.296385 5:-22385804 MsG0580025670.01.T01:CDS
GGTGGAGTGGCTGCAGGTGC+TGG 0.307894 5:-22385836 MsG0580025670.01.T01:CDS
GGACCACCGGGACCGGGTGC+TGG 0.344564 5:-22386016 MsG0580025670.01.T01:CDS
GGAGAGGAGGAAGAAGAATC+TGG 0.365531 5:-22385992 MsG0580025670.01.T01:CDS
GGGTGCTGGACCACCGGGAC+CGG 0.366168 5:-22386023 MsG0580025670.01.T01:CDS
GGTGGAGTTGGGGTTGCTGA+TGG 0.374217 5:-22385794 MsG0580025670.01.T01:CDS
CCACCGTTGGGACCGGGTGC+TGG 0.386430 5:-22386037 MsG0580025670.01.T01:CDS
CTCTGCTGCTTTCTTCGCCA+TGG 0.396906 5:+22386132 None:intergenic
GCTGTGGGTGGAGTGGCTGC+AGG 0.406481 5:-22385842 MsG0580025670.01.T01:CDS
GGGACCGGGTGCTGGACCAC+CGG 0.415259 5:-22386029 MsG0580025670.01.T01:CDS
GGTGCTGGAGGGGAAGTTGA+TGG 0.429624 5:-22385944 MsG0580025670.01.T01:CDS
TCTCCTCCAGCACCCGGTCC+CGG 0.431458 5:+22386010 None:intergenic
GGAGTTGGGGTTGCTGATGG+TGG 0.434417 5:-22385791 MsG0580025670.01.T01:CDS
AGCTGTTGGTGGAGTGGCTG+TGG 0.434592 5:-22385873 MsG0580025670.01.T01:CDS
GTTGCTGATGGTGGTTGTGT+TGG 0.437158 5:-22385782 MsG0580025670.01.T01:CDS
GCTGTGGGTGGAGTGGCTGT+GGG 0.444542 5:-22385857 MsG0580025670.01.T01:CDS
GGTTGTGTTGGTGCAGCACC+TGG 0.446993 5:-22385770 MsG0580025670.01.T01:CDS
GTGGGTGGAGTGGCTGTGGG+TGG 0.452550 5:-22385854 MsG0580025670.01.T01:CDS
TGGAGTGGCTGTGGGTGGAG+TGG 0.458963 5:-22385849 MsG0580025670.01.T01:CDS
GGAAGTTGATGGTGCAGGAG+TGG 0.459049 5:-22385933 MsG0580025670.01.T01:CDS
AGCACCACCACCGTTGGGAC+CGG 0.467156 5:-22386044 MsG0580025670.01.T01:CDS
GCAGTTGGTGGTGTTGCTGT+TGG 0.475283 5:-22385902 MsG0580025670.01.T01:CDS
TCCTCCTCTCCTCCAGCACC+CGG 0.477704 5:+22386004 None:intergenic
TGGAGTGGCTGTGGGTGGAG+TGG 0.484799 5:-22385864 MsG0580025670.01.T01:CDS
GTGGGAGCAGCTGCAGTTGG+TGG 0.484852 5:-22385914 MsG0580025670.01.T01:CDS
GGACCGGGTGCTGGACCACC+GGG 0.491197 5:-22386028 MsG0580025670.01.T01:CDS
GAAGTTGATGGTGCAGGAGT+GGG 0.494475 5:-22385932 MsG0580025670.01.T01:CDS
AATGCTGCTGAGGGTGAAGG+TGG 0.499006 5:-22386073 MsG0580025670.01.T01:CDS
CCCAACGGTGGTGGTGCTGA+TGG 0.500063 5:+22386049 None:intergenic
GCTGTTGGTGGAGTGGCTGT+GGG 0.503159 5:-22385872 MsG0580025670.01.T01:CDS
GGTGCTGGACCACCGGGACC+GGG 0.503255 5:-22386022 MsG0580025670.01.T01:CDS
CCATCAGCACCACCACCGTT+GGG 0.510123 5:-22386049 MsG0580025670.01.T01:CDS
CTCCTTCAACAAACCCATCA+TGG 0.511324 5:+22386096 None:intergenic
GAGAGCAGTTGCTGCCATGA+TGG 0.511561 5:-22386110 MsG0580025670.01.T01:CDS
TGCCATGATGGGTTTGTTGA+AGG 0.512299 5:-22386098 MsG0580025670.01.T01:CDS
GAAGTAGGTGTTGGTGCTGG+AGG 0.514141 5:-22385956 MsG0580025670.01.T01:CDS
GGAGTGGGTGCTTCAGAAGT+AGG 0.515292 5:-22385971 MsG0580025670.01.T01:CDS
GGAGTGGGAGCAGCTGCAGT+TGG 0.520325 5:-22385917 MsG0580025670.01.T01:CDS
GTTGGTGGTGTTGCTGTTGG+AGG 0.520521 5:-22385899 MsG0580025670.01.T01:CDS
GTTGGTGGAGTGGCTGTGGG+TGG 0.521906 5:-22385869 MsG0580025670.01.T01:CDS
CCACCGGGACCGGGTGCTGG+AGG 0.523320 5:-22386013 MsG0580025670.01.T01:CDS
AGAGCAGTTGCTGCCATGAT+GGG 0.526905 5:-22386109 MsG0580025670.01.T01:CDS
GCACCACCACCGTTGGGACC+GGG 0.528708 5:-22386043 MsG0580025670.01.T01:CDS
GCACCCGGTCCCAACGGTGG+TGG 0.530310 5:+22386040 None:intergenic
ACCGGGTGCTGGAGGAGAGG+AGG 0.532438 5:-22386005 MsG0580025670.01.T01:CDS
GGAGGGGAAGTTGATGGTGC+AGG 0.532688 5:-22385938 MsG0580025670.01.T01:CDS
GGAGGAAGAAGAATCTGGAG+TGG 0.532792 5:-22385987 MsG0580025670.01.T01:CDS
TCAGAAGTAGGTGTTGGTGC+TGG 0.532872 5:-22385959 MsG0580025670.01.T01:CDS
GGGACCGGGTGCTGGAGGAG+AGG 0.539714 5:-22386008 MsG0580025670.01.T01:CDS
GCCATCAGCACCACCACCGT+TGG 0.546879 5:-22386050 MsG0580025670.01.T01:CDS
AGTAGGTGTTGGTGCTGGAG+GGG 0.555853 5:-22385954 MsG0580025670.01.T01:CDS
AAGTAGGTGTTGGTGCTGGA+GGG 0.566401 5:-22385955 MsG0580025670.01.T01:CDS
GTTGGAGGAGTAGCTGTTGG+TGG 0.571740 5:-22385884 MsG0580025670.01.T01:CDS
GGTCCCGGTGGTCCAGCACC+CGG 0.574417 5:+22386025 None:intergenic
GGTCCAGCACCCGGTCCCAA+CGG 0.575184 5:+22386034 None:intergenic
AGGAGTAGCTGTTGGTGGAG+TGG 0.587143 5:-22385879 MsG0580025670.01.T01:CDS
GCTGTTGGAGGAGTAGCTGT+TGG 0.592294 5:-22385887 MsG0580025670.01.T01:CDS
CCAGCACCCGGTCCCAACGG+TGG 0.598882 5:+22386037 None:intergenic
GAGGAAGAAGAATCTGGAGT+GGG 0.600175 5:-22385986 MsG0580025670.01.T01:CDS
GAGAATGCTGCTGAGGGTGA+AGG 0.610127 5:-22386076 MsG0580025670.01.T01:CDS
GTTGAAGGAGAATGCTGCTG+AGG 0.619792 5:-22386083 MsG0580025670.01.T01:CDS
GGTGCTTCAGAAGTAGGTGT+TGG 0.633700 5:-22385965 MsG0580025670.01.T01:CDS
CCTCCAGCACCCGGTCCCGG+TGG 0.634291 5:+22386013 None:intergenic
TTGAAGGAGAATGCTGCTGA+GGG 0.647476 5:-22386082 MsG0580025670.01.T01:CDS
TGCTTGTGCAAAACAAGAGG+TGG 0.656798 5:-22385747 MsG0580025670.01.T01:CDS
TGGTGCTTGTGCAAAACAAG+AGG 0.690722 5:-22385750 MsG0580025670.01.T01:CDS
ATGCTGCTGAGGGTGAAGGT+GGG 0.701564 5:-22386072 MsG0580025670.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTACAAGTTTTTCTCATCTT+AGG + Chr5:22386123-22386142 None:intergenic 30.0%
CTCCTTCAACAAACCCATCA+TGG + Chr5:22385743-22385762 None:intergenic 45.0%
TGCTTGTGCAAAACAAGAGG+TGG - Chr5:22386089-22386108 MsG0580025670.01.T01:CDS 45.0%
TGGTGCTTGTGCAAAACAAG+AGG - Chr5:22386086-22386105 MsG0580025670.01.T01:CDS 45.0%
TTGAAGGAGAATGCTGCTGA+GGG - Chr5:22385754-22385773 MsG0580025670.01.T01:CDS 45.0%
! GAGGAAGAAGAATCTGGAGT+GGG - Chr5:22385850-22385869 MsG0580025670.01.T01:CDS 45.0%
!! TGCCATGATGGGTTTGTTGA+AGG - Chr5:22385738-22385757 MsG0580025670.01.T01:CDS 45.0%
!!! TCTTGTTTTGCACAAGCACC+AGG + Chr5:22386087-22386106 None:intergenic 45.0%
AGAGCAGTTGCTGCCATGAT+GGG - Chr5:22385727-22385746 MsG0580025670.01.T01:CDS 50.0%
GGAGAGGAGGAAGAAGAATC+TGG - Chr5:22385844-22385863 MsG0580025670.01.T01:CDS 50.0%
GGAGGAAGAAGAATCTGGAG+TGG - Chr5:22385849-22385868 MsG0580025670.01.T01:CDS 50.0%
GGTGCTTCAGAAGTAGGTGT+TGG - Chr5:22385871-22385890 MsG0580025670.01.T01:CDS 50.0%
GTTGAAGGAGAATGCTGCTG+AGG - Chr5:22385753-22385772 MsG0580025670.01.T01:CDS 50.0%
!! AAGTAGGTGTTGGTGCTGGA+GGG - Chr5:22385881-22385900 MsG0580025670.01.T01:CDS 50.0%
!! GAAGTTGATGGTGCAGGAGT+GGG - Chr5:22385904-22385923 MsG0580025670.01.T01:CDS 50.0%
!! GTTGCTGATGGTGGTTGTGT+TGG - Chr5:22386054-22386073 MsG0580025670.01.T01:CDS 50.0%
!! TCAGAAGTAGGTGTTGGTGC+TGG - Chr5:22385877-22385896 MsG0580025670.01.T01:CDS 50.0%
!!! GTGCTGGAGATTTTGGTGCT+GGG - Chr5:22386016-22386035 MsG0580025670.01.T01:CDS 50.0%
!!! TGCTGGAGATTTTGGTGCTG+GGG - Chr5:22386017-22386036 MsG0580025670.01.T01:CDS 50.0%
AATGCTGCTGAGGGTGAAGG+TGG - Chr5:22385763-22385782 MsG0580025670.01.T01:CDS 55.0%
ATGCTGCTGAGGGTGAAGGT+GGG - Chr5:22385764-22385783 MsG0580025670.01.T01:CDS 55.0%
GAGAATGCTGCTGAGGGTGA+AGG - Chr5:22385760-22385779 MsG0580025670.01.T01:CDS 55.0%
GAGAGCAGTTGCTGCCATGA+TGG - Chr5:22385726-22385745 MsG0580025670.01.T01:CDS 55.0%
GCTGTTGGAGGAGTAGCTGT+TGG - Chr5:22385949-22385968 MsG0580025670.01.T01:CDS 55.0%
GGAGTGGGTGCTTCAGAAGT+AGG - Chr5:22385865-22385884 MsG0580025670.01.T01:CDS 55.0%
GTTGGAGGAGTAGCTGTTGG+TGG - Chr5:22385952-22385971 MsG0580025670.01.T01:CDS 55.0%
! AGGAGTAGCTGTTGGTGGAG+TGG - Chr5:22385957-22385976 MsG0580025670.01.T01:CDS 55.0%
!! AGTAGGTGTTGGTGCTGGAG+GGG - Chr5:22385882-22385901 MsG0580025670.01.T01:CDS 55.0%
!! GAAGTAGGTGTTGGTGCTGG+AGG - Chr5:22385880-22385899 MsG0580025670.01.T01:CDS 55.0%
!! GCAGTTGGTGGTGTTGCTGT+TGG - Chr5:22385934-22385953 MsG0580025670.01.T01:CDS 55.0%
!! GGAAGTTGATGGTGCAGGAG+TGG - Chr5:22385903-22385922 MsG0580025670.01.T01:CDS 55.0%
!! GGTGCTGGAGATTTTGGTGC+TGG - Chr5:22386015-22386034 MsG0580025670.01.T01:CDS 55.0%
!! GTTGGTGGTGTTGCTGTTGG+AGG - Chr5:22385937-22385956 MsG0580025670.01.T01:CDS 55.0%
!!! GATTTTGGTGCTGGGGTTGG+TGG - Chr5:22386024-22386043 MsG0580025670.01.T01:CDS 55.0%
!!! GCTGCAGGTGCTGGAGATTT+TGG - Chr5:22386009-22386028 MsG0580025670.01.T01:CDS 55.0%
!!! GGAGATTTTGGTGCTGGGGT+TGG - Chr5:22386021-22386040 MsG0580025670.01.T01:CDS 55.0%
CCATCAGCACCACCACCGTT+GGG - Chr5:22385787-22385806 MsG0580025670.01.T01:CDS 60.0%
GGAGTTGGGGTTGCTGATGG+TGG - Chr5:22386045-22386064 MsG0580025670.01.T01:CDS 60.0%
GGTGGAGTTGGGGTTGCTGA+TGG - Chr5:22386042-22386061 MsG0580025670.01.T01:CDS 60.0%
! AGCTGTTGGTGGAGTGGCTG+TGG - Chr5:22385963-22385982 MsG0580025670.01.T01:CDS 60.0%
! GCTGTTGGTGGAGTGGCTGT+GGG - Chr5:22385964-22385983 MsG0580025670.01.T01:CDS 60.0%
! GGTGCTGGAGGGGAAGTTGA+TGG - Chr5:22385892-22385911 MsG0580025670.01.T01:CDS 60.0%
! GGTTGTGTTGGTGCAGCACC+TGG - Chr5:22386066-22386085 MsG0580025670.01.T01:CDS 60.0%
!! GGAGGGGAAGTTGATGGTGC+AGG - Chr5:22385898-22385917 MsG0580025670.01.T01:CDS 60.0%
!! GTGCTGGGGTTGGTGGAGTT+GGG - Chr5:22386031-22386050 MsG0580025670.01.T01:CDS 60.0%
!! TGCTGGGGTTGGTGGAGTTG+GGG - Chr5:22386032-22386051 MsG0580025670.01.T01:CDS 60.0%
AGCACCACCACCGTTGGGAC+CGG - Chr5:22385792-22385811 MsG0580025670.01.T01:CDS 65.0%
GCCATCAGCACCACCACCGT+TGG - Chr5:22385786-22385805 MsG0580025670.01.T01:CDS 65.0%
GCTGTGGGTGGAGTGGCTGT+GGG - Chr5:22385979-22385998 MsG0580025670.01.T01:CDS 65.0%
GGAGTGGGAGCAGCTGCAGT+TGG - Chr5:22385919-22385938 MsG0580025670.01.T01:CDS 65.0%
GTGGGAGCAGCTGCAGTTGG+TGG - Chr5:22385922-22385941 MsG0580025670.01.T01:CDS 65.0%
TCCTCCTCTCCTCCAGCACC+CGG + Chr5:22385835-22385854 None:intergenic 65.0%
TGGAGTGGCTGTGGGTGGAG+TGG - Chr5:22385972-22385991 MsG0580025670.01.T01:CDS 65.0%
! CCCAACGGTGGTGGTGCTGA+TGG + Chr5:22385790-22385809 None:intergenic 65.0%
! GTTGGTGGAGTGGCTGTGGG+TGG - Chr5:22385967-22385986 MsG0580025670.01.T01:CDS 65.0%
!! GGTGCTGGGGTTGGTGGAGT+TGG - Chr5:22386030-22386049 MsG0580025670.01.T01:CDS 65.0%
GCACCACCACCGTTGGGACC+GGG - Chr5:22385793-22385812 MsG0580025670.01.T01:CDS 70.0%
GCTGTGGGTGGAGTGGCTGC+AGG - Chr5:22385994-22386013 MsG0580025670.01.T01:CDS 70.0%
GGCTGTGGGTGGAGTGGCTG+TGG - Chr5:22385978-22385997 MsG0580025670.01.T01:CDS 70.0%
GGTCCAGCACCCGGTCCCAA+CGG + Chr5:22385805-22385824 None:intergenic 70.0%
GGTGGAGTGGCTGCAGGTGC+TGG - Chr5:22386000-22386019 MsG0580025670.01.T01:CDS 70.0%
GTGGGTGGAGTGGCTGTGGG+TGG - Chr5:22385982-22386001 MsG0580025670.01.T01:CDS 70.0%
TCTCCTCCAGCACCCGGTCC+CGG + Chr5:22385829-22385848 None:intergenic 70.0%
!! ACCGGGTGCTGGAGGAGAGG+AGG - Chr5:22385831-22385850 MsG0580025670.01.T01:CDS 70.0%
CCACCGTTGGGACCGGGTGC+TGG - Chr5:22385799-22385818 MsG0580025670.01.T01:CDS 75.0%
CCAGCACCCGGTCCCAACGG+TGG + Chr5:22385802-22385821 None:intergenic 75.0%
GCACCCGGTCCCAACGGTGG+TGG + Chr5:22385799-22385818 None:intergenic 75.0%
GGTCCCGGTGGTCCAGCACC+CGG + Chr5:22385814-22385833 None:intergenic 75.0%
! GGTGCTGGACCACCGGGACC+GGG - Chr5:22385814-22385833 MsG0580025670.01.T01:CDS 75.0%
!! GGACCGGGTGCTGGACCACC+GGG - Chr5:22385808-22385827 MsG0580025670.01.T01:CDS 75.0%
!! GGGACCGGGTGCTGGACCAC+CGG - Chr5:22385807-22385826 MsG0580025670.01.T01:CDS 75.0%
!! GGGACCGGGTGCTGGAGGAG+AGG - Chr5:22385828-22385847 MsG0580025670.01.T01:CDS 75.0%
!! GGGTGCTGGACCACCGGGAC+CGG - Chr5:22385813-22385832 MsG0580025670.01.T01:CDS 75.0%
! CCTCCAGCACCCGGTCCCGG+TGG + Chr5:22385826-22385845 None:intergenic 80.0%
! GGACCACCGGGACCGGGTGC+TGG - Chr5:22385820-22385839 MsG0580025670.01.T01:CDS 80.0%
!!! CCACCGGGACCGGGTGCTGG+AGG - Chr5:22385823-22385842 MsG0580025670.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr5 gene 22385706 22386152 22385706 ID=MsG0580025670.01;Name=MsG0580025670.01
Chr5 mRNA 22385706 22386152 22385706 ID=MsG0580025670.01.T01;Parent=MsG0580025670.01;Name=MsG0580025670.01.T01;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|148
Chr5 exon 22385706 22386152 22385706 ID=MsG0580025670.01.T01:exon:9372;Parent=MsG0580025670.01.T01
Chr5 CDS 22385706 22386152 22385706 ID=MsG0580025670.01.T01:cds;Parent=MsG0580025670.01.T01
Gene Sequence

>MsG0580025670.01.T01

ATGGCGAAGAAAGCAGCAGAGAGAGCAGTTGCTGCCATGATGGGTTTGTTGAAGGAGAATGCTGCTGAGGGTGAAGGTGGGCCATCAGCACCACCACCGTTGGGACCGGGTGCTGGACCACCGGGACCGGGTGCTGGAGGAGAGGAGGAAGAAGAATCTGGAGTGGGTGCTTCAGAAGTAGGTGTTGGTGCTGGAGGGGAAGTTGATGGTGCAGGAGTGGGAGCAGCTGCAGTTGGTGGTGTTGCTGTTGGAGGAGTAGCTGTTGGTGGAGTGGCTGTGGGTGGAGTGGCTGTGGGTGGAGTGGCTGCAGGTGCTGGAGATTTTGGTGCTGGGGTTGGTGGAGTTGGGGTTGCTGATGGTGGTTGTGTTGGTGCAGCACCTGGTGCTTGTGCAAAACAAGAGGTGGCTAGAAGACCTAAGATGAGAAAAACTTGTACTGCTGCATAG

Protein sequence

>MsG0580025670.01.T01

MAKKAAERAVAAMMGLLKENAAEGEGGPSAPPPLGPGAGPPGPGAGGEEEEESGVGASEVGVGAGGEVDGAGVGAAAVGGVAVGGVAVGGVAVGGVAVGGVAAGAGDFGAGVGGVGVADGGCVGAAPGACAKQEVARRPKMRKTCTAA*