AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001691.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001691.01.T01 MTR_1g041285 55.333 150 22 5 1 105 1 150 1.14e-36 121
MsG0180001691.01.T01 MTR_1g076650 45.638 149 36 5 1 104 1 149 1.84e-26 95.9
MsG0180001691.01.T01 MTR_6g025320 43.243 148 39 4 1 103 1 148 4.62e-26 94.7
MsG0180001691.01.T01 MTR_7g087610 42.568 148 40 4 1 103 1 148 9.56e-26 94.0
MsG0180001691.01.T01 MTR_5g088320 42.568 148 40 4 1 103 1 148 2.96e-25 92.8
MsG0180001691.01.T01 MTR_0168s0120 44.954 109 28 3 27 103 4 112 7.90e-20 77.8
MsG0180001691.01.T01 MTR_6g025320 44.954 109 28 3 27 103 4 112 7.90e-20 77.8
MsG0180001691.01.T01 MTR_1g071150 38.356 146 46 4 1 102 1 146 1.12e-17 73.2
MsG0180001691.01.T01 MTR_7g115040 35.570 149 51 4 1 104 1 149 3.84e-14 64.3
MsG0180001691.01.T01 MTR_3g055510 39.394 99 52 3 1 99 41 131 6.23e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001691.01.T01 AT2G27030 43.919 148 38 4 1 103 1 148 2.29e-26 96.7
MsG0180001691.01.T01 AT3G56800 43.919 148 38 4 1 103 1 148 2.36e-26 95.5
MsG0180001691.01.T01 AT2G27030 43.919 148 38 4 1 103 1 148 2.36e-26 95.5
MsG0180001691.01.T01 AT2G41110 43.919 148 38 4 1 103 1 148 2.36e-26 95.5
MsG0180001691.01.T01 AT3G43810 43.243 148 39 4 1 103 1 148 4.83e-26 94.7
MsG0180001691.01.T01 AT5G21274 42.568 148 40 4 1 103 1 148 1.39e-25 93.6
MsG0180001691.01.T01 AT3G43810 43.243 148 39 4 1 103 1 148 1.47e-25 95.5
MsG0180001691.01.T01 AT5G37780 41.892 148 41 4 1 103 1 148 1.84e-24 90.9
MsG0180001691.01.T01 AT1G66410 41.892 148 41 4 1 103 1 148 1.84e-24 90.9
MsG0180001691.01.T01 AT3G22930 43.836 146 37 5 5 105 28 173 2.55e-24 91.3
MsG0180001691.01.T01 AT2G41110 40.625 160 38 4 1 103 1 160 3.03e-24 90.5
MsG0180001691.01.T01 AT4G14640 42.759 145 38 5 5 104 6 150 4.44e-23 87.4
MsG0180001691.01.T01 AT5G37780 38.037 163 41 4 1 103 1 163 7.04e-22 84.7
MsG0180001691.01.T01 AT1G66410 39.241 158 41 4 1 103 1 158 3.88e-21 82.4
MsG0180001691.01.T01 AT5G37780 36.207 174 40 5 1 103 1 174 2.53e-20 80.9
MsG0180001691.01.T01 AT3G43810 44.954 109 28 3 27 103 4 112 8.28e-20 77.8
MsG0180001691.01.T01 AT3G43810 44.954 109 28 3 27 103 4 112 8.28e-20 77.8
MsG0180001691.01.T01 AT1G66410 44.037 109 29 3 27 103 4 112 4.98e-19 75.9
MsG0180001691.01.T01 AT1G66410 44.037 109 29 3 27 103 4 112 4.98e-19 75.9
MsG0180001691.01.T01 AT5G37780 44.037 109 29 3 27 103 27 135 8.74e-19 75.9
MsG0180001691.01.T01 AT4G14640 86.486 37 5 0 68 104 96 132 2.43e-15 67.0
MsG0180001691.01.T01 AT2G41090 31.724 145 57 4 1 103 1 145 1.67e-12 60.8
MsG0180001691.01.T01 AT3G51920 31.081 148 57 4 1 103 1 148 4.54e-11 56.2

Find 22 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATTGAATTTGTTGATTC+TGG 0.176831 1:+25390064 MsG0180001691.01.T01:CDS
CAACCTCGTTTATCATTTCT+TGG 0.231199 1:-25390025 None:intergenic
CTTCTTTGATCTCAACAATT+TGG 0.239416 1:-25389678 None:intergenic
TTGTTGATTCTGGAACTTAA+TGG 0.285580 1:+25390074 MsG0180001691.01.T01:CDS
GATGATTAAAGAAGCAGATT+TGG 0.325770 1:+25391383 MsG0180001691.01.T01:CDS
TTGAGACAAGTATGATCAAT+TGG 0.348422 1:+25391329 MsG0180001691.01.T01:intron
TTCTTGGAGCTCTCCTCTGT+TGG 0.358344 1:-25390009 None:intergenic
TGCAACTGTAATTAGGTCAT+TGG 0.376466 1:+25389978 MsG0180001691.01.T01:intron
TCTTGGAGCTCTCCTCTGTT+GGG 0.377584 1:-25390008 None:intergenic
TCCAGAATCAACAAATTCAA+TGG 0.435475 1:-25390065 None:intergenic
CATCTTGTCAAACAAGCAAA+AGG 0.480213 1:-25389701 None:intergenic
GGATAGTTTATTTCATCCAA+TGG 0.496741 1:-25391454 None:intergenic
CGAGGTTGATGCAGATGGAA+TGG 0.522058 1:+25390040 MsG0180001691.01.T01:CDS
GTTAAGATGATGATGACCAT+TGG 0.525117 1:+25391438 MsG0180001691.01.T01:CDS
GAAGCAGATTTGGACGGTGA+TGG 0.535179 1:+25391393 MsG0180001691.01.T01:CDS
TGAGACAAGTATGATCAATT+GGG 0.537602 1:+25391330 MsG0180001691.01.T01:intron
ATAAACGAGGTTGATGCAGA+TGG 0.555436 1:+25390035 MsG0180001691.01.T01:CDS
CTTGTTTGACAAGATGGAGA+TGG 0.585467 1:+25389708 MsG0180001691.01.T01:CDS
AAAATTAACTGATGAAGAGG+TGG 0.600647 1:+25391356 MsG0180001691.01.T01:CDS
ATTAAAGAAGCAGATTTGGA+CGG 0.618310 1:+25391387 MsG0180001691.01.T01:CDS
GCTCCAAGAAATGATAAACG+AGG 0.724641 1:+25390022 MsG0180001691.01.T01:CDS
ATTGGATCAAAACCCAACAG+AGG 0.725937 1:+25389996 MsG0180001691.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATTGAAATGAAATAT+GGG - Chr1:25389767-25389786 None:intergenic 10.0%
!! TATATAATTGAAATGAAATA+TGG - Chr1:25389768-25389787 None:intergenic 10.0%
!!! AATTTTTTTCATAGTTTTAA+TGG - Chr1:25390921-25390940 None:intergenic 10.0%
!! ATAACAAAGAAGTTAAATAA+GGG - Chr1:25390659-25390678 None:intergenic 15.0%
!! CTTTATGATTAATCTTTAAT+AGG + Chr1:25389934-25389953 MsG0180001691.01.T01:intron 15.0%
!! TATAACAAAGAAGTTAAATA+AGG - Chr1:25390660-25390679 None:intergenic 15.0%
!! TATAATTGAAATGAAATATG+GGG - Chr1:25389766-25389785 None:intergenic 15.0%
!!! ACAATTTTCATATTAGTTTA+AGG - Chr1:25389866-25389885 None:intergenic 15.0%
!!! ACAATTTTTTTTCTTGTATA+TGG + Chr1:25390555-25390574 MsG0180001691.01.T01:intron 15.0%
!!! TTATTTAACTTCTTTGTTAT+AGG + Chr1:25390659-25390678 MsG0180001691.01.T01:intron 15.0%
!! AAAAATTTATTGAAGCATAG+AGG + Chr1:25390934-25390953 MsG0180001691.01.T01:intron 20.0%
!! ATGTATTATGATAGATTTCA+TGG + Chr1:25390593-25390612 MsG0180001691.01.T01:intron 20.0%
!! CAACAAAAAAAAAAACTTCA+TGG + Chr1:25390630-25390649 MsG0180001691.01.T01:intron 20.0%
!! CATTATTGTAAGAAATGAAA+AGG - Chr1:25390126-25390145 None:intergenic 20.0%
!! TAACAAAGAAGTTAAATAAG+GGG - Chr1:25390658-25390677 None:intergenic 20.0%
!! TAATACTGTATAAGTGTAAT+GGG - Chr1:25390527-25390546 None:intergenic 20.0%
!! TACACTTATACAGTATTAAA+TGG + Chr1:25390529-25390548 MsG0180001691.01.T01:intron 20.0%
!! TTAATACTGTATAAGTGTAA+TGG - Chr1:25390528-25390547 None:intergenic 20.0%
!!! GAATAATTACTTTCATTTTC+TGG - Chr1:25390100-25390119 None:intergenic 20.0%
!!! GGTTTTTGATAAAGATAAAA+TGG + Chr1:25390236-25390255 MsG0180001691.01.T01:intron 20.0%
!!! TTTTCTTGTATATGGAAAAA+AGG + Chr1:25390563-25390582 MsG0180001691.01.T01:intron 20.0%
!!! TTTTTTTGTTGAATGAATGA+GGG - Chr1:25390621-25390640 None:intergenic 20.0%
!!! TTTTTTTTGTTGAATGAATG+AGG - Chr1:25390622-25390641 None:intergenic 20.0%
! AAAAAAATCTCTTGCAACAA+AGG - Chr1:25390986-25391005 None:intergenic 25.0%
! AACAAAGAAGTTAAATAAGG+GGG - Chr1:25390657-25390676 None:intergenic 25.0%
! ACGAGTTTCACATAATTATA+CGG - Chr1:25390387-25390406 None:intergenic 25.0%
! ATATTGTCAACAATCAAAGA+AGG - Chr1:25389801-25389820 None:intergenic 25.0%
! GAAAAACATTCAACTTCTAT+AGG - Chr1:25391010-25391029 None:intergenic 25.0%
! TGAAAAATTAACTGATGAAG+AGG + Chr1:25391353-25391372 MsG0180001691.01.T01:CDS 25.0%
!! CTATTATTATTCATCGATTG+CGG - Chr1:25391158-25391177 None:intergenic 25.0%
!! TAACTTCTTTGTTATAGGAT+TGG + Chr1:25390664-25390683 MsG0180001691.01.T01:intron 25.0%
!! TATATCAATATTTTCCTCTC+CGG + Chr1:25390279-25390298 MsG0180001691.01.T01:intron 25.0%
AAAATTAACTGATGAAGAGG+TGG + Chr1:25391356-25391375 MsG0180001691.01.T01:CDS 30.0%
AAGAACTTGCAACTGTAATT+AGG + Chr1:25389971-25389990 MsG0180001691.01.T01:intron 30.0%
ACAAAGAAGTTAAATAAGGG+GGG - Chr1:25390656-25390675 None:intergenic 30.0%
ATATTGATATACCTCACTAG+CGG - Chr1:25390270-25390289 None:intergenic 30.0%
ATGCACATTAATTTCACACA+TGG + Chr1:25391064-25391083 MsG0180001691.01.T01:intron 30.0%
ATTAAAGAAGCAGATTTGGA+CGG + Chr1:25391387-25391406 MsG0180001691.01.T01:CDS 30.0%
CACATACACACATATAAGTT+TGG + Chr1:25389889-25389908 MsG0180001691.01.T01:intron 30.0%
CTTCTTTGATCTCAACAATT+TGG - Chr1:25389681-25389700 None:intergenic 30.0%
GATGATTAAAGAAGCAGATT+TGG + Chr1:25391383-25391402 MsG0180001691.01.T01:CDS 30.0%
GTTATCTAAACATGCATAAC+CGG - Chr1:25390301-25390320 None:intergenic 30.0%
TCCAGAATCAACAAATTCAA+TGG - Chr1:25390068-25390087 None:intergenic 30.0%
TGAGACAAGTATGATCAATT+GGG + Chr1:25391330-25391349 MsG0180001691.01.T01:intron 30.0%
TTGAGACAAGTATGATCAAT+TGG + Chr1:25391329-25391348 MsG0180001691.01.T01:intron 30.0%
TTTAATAGGTTGCATTACTG+TGG + Chr1:25389948-25389967 MsG0180001691.01.T01:intron 30.0%
! AAGAAAAAAGTGTGATGGAA+AGG - Chr1:25390493-25390512 None:intergenic 30.0%
! CACTTAAGAAAAAAGTGTGA+TGG - Chr1:25390498-25390517 None:intergenic 30.0%
!! ACCATTGAATTTGTTGATTC+TGG + Chr1:25390064-25390083 MsG0180001691.01.T01:CDS 30.0%
!! TTGTTGATTCTGGAACTTAA+TGG + Chr1:25390074-25390093 MsG0180001691.01.T01:CDS 30.0%
ACACTTCTTCAATCAACTAG+AGG + Chr1:25391114-25391133 MsG0180001691.01.T01:intron 35.0%
CAACCTCGTTTATCATTTCT+TGG - Chr1:25390028-25390047 None:intergenic 35.0%
CATCTTGTCAAACAAGCAAA+AGG - Chr1:25389704-25389723 None:intergenic 35.0%
GAAGATCTCAAAGAGCTTTA+AGG + Chr1:25390215-25390234 MsG0180001691.01.T01:intron 35.0%
TGCAACTGTAATTAGGTCAT+TGG + Chr1:25389978-25389997 MsG0180001691.01.T01:intron 35.0%
! ATTATTCATCGATTGCGGAA+AGG - Chr1:25391153-25391172 None:intergenic 35.0%
! CTTTTGCTTGTTTGACAAGA+TGG + Chr1:25389702-25389721 MsG0180001691.01.T01:CDS 35.0%
! GTTAAGATGATGATGACCAT+TGG + Chr1:25391438-25391457 MsG0180001691.01.T01:CDS 35.0%
ATCTCTTGCAACAAAGGAAC+AGG - Chr1:25390980-25390999 None:intergenic 40.0%
ATTGGATCAAAACCCAACAG+AGG + Chr1:25389996-25390015 MsG0180001691.01.T01:CDS 40.0%
CTGCATCAGTTTCCAAATGA+GGG - Chr1:25390193-25390212 None:intergenic 40.0%
CTTGTTTGACAAGATGGAGA+TGG + Chr1:25389708-25389727 MsG0180001691.01.T01:CDS 40.0%
GCTCCAAGAAATGATAAACG+AGG + Chr1:25390022-25390041 MsG0180001691.01.T01:CDS 40.0%
TCGTTGTCATGCAATTGTGT+AGG + Chr1:25390403-25390422 MsG0180001691.01.T01:intron 40.0%
TCTGCATCAGTTTCCAAATG+AGG - Chr1:25390194-25390213 None:intergenic 40.0%
TGCATCAGTTTCCAAATGAG+GGG - Chr1:25390192-25390211 None:intergenic 40.0%
TGGTTATATTTCCGCTAGTG+AGG + Chr1:25390256-25390275 MsG0180001691.01.T01:intron 40.0%
!! ATAAACGAGGTTGATGCAGA+TGG + Chr1:25390035-25390054 MsG0180001691.01.T01:CDS 40.0%
!! TCTATTTTTCCCCCCTCATT+TGG + Chr1:25390178-25390197 MsG0180001691.01.T01:intron 40.0%
CATCAGTTTCCAAATGAGGG+GGG - Chr1:25390190-25390209 None:intergenic 45.0%
CTAAACATGCATAACCGGAG+AGG - Chr1:25390296-25390315 None:intergenic 45.0%
GCATCAGTTTCCAAATGAGG+GGG - Chr1:25390191-25390210 None:intergenic 45.0%
!!! AATAATAAAAAAATTTTTTG+AGG + Chr1:25390828-25390847 MsG0180001691.01.T01:intron 5.0%
!!! ATAATAAAAAAATTTTTTGA+GGG + Chr1:25390829-25390848 MsG0180001691.01.T01:intron 5.0%
GAAGCAGATTTGGACGGTGA+TGG + Chr1:25391393-25391412 MsG0180001691.01.T01:CDS 50.0%
TCTTGGAGCTCTCCTCTGTT+GGG - Chr1:25390011-25390030 None:intergenic 50.0%
TTCTTGGAGCTCTCCTCTGT+TGG - Chr1:25390012-25390031 None:intergenic 50.0%
!! CGAGGTTGATGCAGATGGAA+TGG + Chr1:25390040-25390059 MsG0180001691.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 25389655 25391464 25389655 ID=MsG0180001691.01;Name=MsG0180001691.01
Chr1 mRNA 25389655 25391464 25389655 ID=MsG0180001691.01.T01;Parent=MsG0180001691.01;Name=MsG0180001691.01.T01;_AED=0.24;_eAED=0.37;_QI=0|0|0|1|0|0|3|0|105
Chr1 exon 25389655 25389729 25389655 ID=MsG0180001691.01.T01:exon:18442;Parent=MsG0180001691.01.T01
Chr1 exon 25389993 25390109 25389993 ID=MsG0180001691.01.T01:exon:18443;Parent=MsG0180001691.01.T01
Chr1 exon 25391339 25391464 25391339 ID=MsG0180001691.01.T01:exon:18444;Parent=MsG0180001691.01.T01
Chr1 CDS 25389655 25389729 25389655 ID=MsG0180001691.01.T01:cds;Parent=MsG0180001691.01.T01
Chr1 CDS 25389993 25390109 25389993 ID=MsG0180001691.01.T01:cds;Parent=MsG0180001691.01.T01
Chr1 CDS 25391339 25391464 25391339 ID=MsG0180001691.01.T01:cds;Parent=MsG0180001691.01.T01
Gene Sequence

>MsG0180001691.01.T01

ATGGCAGAAATTTTGAATGAAGACCAAATTGTTGAGATCAAAGAAGCCTTTTGCTTGTTTGACAAGATGGAGATGGTCATTGGATCAAAACCCAACAGAGGAGAGCTCCAAGAAATGATAAACGAGGTTGATGCAGATGGAATGGAACCATTGAATTTGTTGATTCTGGAACTTAATGGCCAGAAAATGAAATATGATCAATTGGGTGAAAAATTAACTGATGAAGAGGTGGATCAGATGATTAAAGAAGCAGATTTGGACGGTGATGGACAAGTGAACTTTGAAGAGTTTGTTAAGATGATGATGACCATTGGATGA

Protein sequence

>MsG0180001691.01.T01

MAEILNEDQIVEIKEAFCLFDKMEMVIGSKPNRGELQEMINEVDADGMEPLNLLILELNGQKMKYDQLGEKLTDEEVDQMIKEADLDGDGQVNFEEFVKMMMTIG*