AlfalfaGEDB | MsG0680035220.01

M. sativa cultivar ZhongmuNo.1 / MsG0680035220.01

KEGG: -
GO: GO:0005515
PFAM: PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035220.01.T01	RHN52157.1	81.996	561	97	3	1	561	53	609	0	935

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035220.01.T01	Q0WKV3	38.06	536	297	1	55	555	69	604	2.17E-137	416

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
MsG0080048802.01	MsG0680035220.01	PPI
MsG0080048802.01	MsG0680035220.01	PPI
MsG0080048802.01	MsG0680035220.01	PPI
MsG0080048925.01	MsG0680035220.01	PPI
MsG0080048926.01	MsG0680035220.01	PPI
MsG0180001046.01	MsG0680035220.01	PPI
MsG0180001047.01	MsG0680035220.01	PPI
MsG0180001883.01	MsG0680035220.01	PPI
MsG0180001883.01	MsG0680035220.01	PPI
MsG0180001883.01	MsG0680035220.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035220.01.T01	MTR_6g065540	81.996	561	97	3	1	561	1	557	0.0	933
MsG0680035220.01.T01	MTR_6g077540	81.469	572	93	4	1	561	1	570	0.0	933
MsG0680035220.01.T01	MTR_6g079830	77.265	563	117	2	8	560	10	571	0.0	891
MsG0680035220.01.T01	MTR_6g079830	30.026	383	268	0	75	457	191	573	2.48e-66	228
MsG0680035220.01.T01	MTR_6g079830	30.081	369	258	0	58	426	209	577	4.18e-52	189
MsG0680035220.01.T01	MTR_6g079830	25.641	351	261	0	211	561	47	397	4.11e-48	177
MsG0680035220.01.T01	MTR_6g079830	27.041	196	143	0	56	251	382	577	2.80e-20	95.1
MsG0680035220.01.T01	MTR_6g079920	76.732	563	120	2	8	560	10	571	0.0	885
MsG0680035220.01.T01	MTR_6g079920	30.311	386	269	0	75	460	191	576	3.67e-67	228
MsG0680035220.01.T01	MTR_6g079920	25.641	351	261	0	211	561	47	397	6.13e-48	176

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035220.01.T01	AT1G12300	38.060	536	297	1	55	555	69	604	2.21e-138	416
MsG0680035220.01.T01	AT1G12300	27.559	381	276	0	180	560	89	469	1.40e-58	206
MsG0680035220.01.T01	AT1G12300	32.174	345	234	0	217	561	91	435	1.56e-54	195
MsG0680035220.01.T01	AT1G12300	29.129	333	236	0	227	559	66	398	8.09e-50	182
MsG0680035220.01.T01	AT1G12300	28.141	199	142	1	362	560	62	259	1.14e-21	99.8
MsG0680035220.01.T01	AT1G62670	38.433	536	295	2	55	555	62	597	1.80e-137	413
MsG0680035220.01.T01	AT1G62670	29.894	378	265	0	180	557	82	459	2.33e-65	224
MsG0680035220.01.T01	AT1G62670	30.790	367	219	2	230	561	62	428	2.54e-58	206
MsG0680035220.01.T01	AT1G62670	30.976	297	205	0	264	560	61	357	1.25e-43	165
MsG0680035220.01.T01	AT1G62670	26.621	293	214	1	268	560	31	322	9.76e-32	130

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Find 94 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGATTCATAGCTTCAT+CGG	0.572127	6:-100750317	None:intergenic
AACATTGACTACATTCATCA+AGG	0.505339	6:+100749643	MsG0680035220.01.T01:CDS
AACTCGTCGGAGCAATTCAA+GGG	0.605482	6:-100749801	None:intergenic
AAGAACAGTGTGGTAATGTT+TGG	0.370051	6:-100749468	None:intergenic
AATAAGGAATGGATTTAAAA+TGG	0.206201	6:-100749310	None:intergenic
AATCTTATTGAATTCGATTG+TGG	0.455826	6:-100749426	None:intergenic
AATTCGATTGTGGGTGGAGT+GGG	0.498127	6:-100749416	None:intergenic
ACAAAACCCTTGAATCATAA+CGG	0.551384	6:-100750701	None:intergenic
ACAGGAACAACATAGTACCT+CGG	0.689676	6:-100749236	None:intergenic
ACATACACTATTCTTATTGA+TGG	0.276400	6:+100750589	MsG0680035220.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAGATTCATAGCTTCAT+CGG	-	Chr6:100750320-100750339	None:intergenic	30.0%
ACAAAACCCTTGAATCATAA+CGG	-	Chr6:100750704-100750723	None:intergenic	30.0%
ACATATACCGTTATGATTCA+AGG	+	Chr6:100750694-100750713	MsG0680035220.01.T01:CDS	30.0%
ACTTACAATTCTCTTATCGA+TGG	+	Chr6:100750379-100750398	MsG0680035220.01.T01:CDS	30.0%
AGCTACAACATCATGATTAA+CGG	+	Chr6:100750274-100750293	MsG0680035220.01.T01:CDS	30.0%
AGTTATGGAACCTTGATTAA+TGG	+	Chr6:100749749-100749768	MsG0680035220.01.T01:CDS	30.0%
ATGTACAATACCGTTATTGA+TGG	+	Chr6:100749854-100749873	MsG0680035220.01.T01:CDS	30.0%
ATGTTGCAATTGACTAAGTT+AGG	-	Chr6:100749530-100749549	None:intergenic	30.0%
CATATACCGTTATGATTCAA+GGG	+	Chr6:100750695-100750714	MsG0680035220.01.T01:CDS	30.0%
CATCAATTCTTTCTGTCAAT+TGG	+	Chr6:100749553-100749572	MsG0680035220.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	100749209	100750894	100749209	ID=MsG0680035220.01;Name=MsG0680035220.01
Chr6	mRNA	100749209	100750894	100749209	ID=MsG0680035220.01.T01;Parent=MsG0680035220.01;Name=MsG0680035220.01.T01;_AED=0.41;_eAED=0.41;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|561
Chr6	exon	100749209	100750894	100749209	ID=MsG0680035220.01.T01:exon:20650;Parent=MsG0680035220.01.T01
Chr6	CDS	100749209	100750894	100749209	ID=MsG0680035220.01.T01:cds;Parent=MsG0680035220.01.T01

Gene Sequence

>MsG0680035220.01.T01

ATGTTTCATTCTTCTTCTTCGATGTTGCCGAGGTACTATGTTGTTCCTGTTTCTCTCTTTGTTCCAAAAAAGTTTCATTCTTTTCAATTTCTGAGAAACACCCATTTTAAATCCATTCCTTATTCTTCAACTTCATCCACATTCCATAATAACAATGATGTTGTTGATGATGCTGTTTCTTTGTTCAATCGCCTGCTTCGTCAGAATCCCACTCCACCCACAATCGAATTCAATAAGATTTTAGGTTCACTTGTCAAATCCAAACATTACCACACTGTTCTTTCCCTTTATCAAAATATGGAATATAGAGGAATTAAACCTAACTTAGTCAATTGCAACATTCTCATCAATTCTTTCTGTCAATTGGGTCTTATCCCTTTTGCTTTCTCTGTATTGACTAAGATTCTCAAAAACGGTTACGAGCCTGATACCATAACATTGACTACATTCATCAAGGGTCTCTGTCTCAAAGGTCAGATCCATCAAGCATTGCATTTTCATGATAAGGTTATTGCGATGGGATTTCATTTGGACCAAGTTAGTTATGGAACCTTGATTAATGGGTTGTGTAAAGTTGGTGAAACAAGAGCAGCCCTTGAATTGCTCCGACGAGTTGATGGTAAATTGGTTCAGCTTAATGCGGTAATGTACAATACCGTTATTGATGGTATGTGTAAAGATAAACACGTTAATGATGCTTTTGATTTATATTCTGAAATGGTTGCTAAGAGAATTTCTCCTAATGTTGTCACTTATAGTGCTTTAATTAGTGGATTTTGCATTGTTGGTAAACTGAGAGATGCAATTGATTTCTTTAATAAAATGATATTCGAAAACATCAACCCAAATGTGTATACTTTTACTATATTGGTTGATGGTTTTTGTAAGGAAGGAAGGGTGAAAGAAGCTAAAATTGTGTTAGCTATGATGATGAAACAAGGCATCAAACCTGATGTTGTTACTTATAACTCTTTAATGGATGGATACTGCCTAGTTAAAGAAGTGAATAAGGCCAAGAGTATATTCAACACTATGTCTCAGAGGGGAATGAATCCTGATATTCACAGCTACAACATCATGATTAACGGGTTTTGTAAGATTAAAATGACCGATGAAGCTATGAATCTCTTTGAAGAAATGCATTGTAGAAAACTAATTCCTGATGTGGTAACTTACAATTCTCTTATCGATGGTTTGTCTAAATCGGGGAAAATCTCTAATGCTTTGAAGCTAGTCGATGAGATGCATGATAGAGGTCAACCACCTGATATAATTACTTACAATTCTATATTAGATGCTTTATGCAAAAACCATCAGGTTGACAAGGCAATTGCAATATTAACAAAATTGAAAGATCAGGGTATTCGACCAAATATGTACACATACACTATTCTTATTGATGGATTGTGTAAAGGTGGAAGACTAAAGGATGCACGGAATATTTTTGAGGATCTTTTAGTCAAGGGCTATAATGTAACTGTCAACACATATACCGTTATGATTCAAGGGTTTTGTAGCCATGGCTTGTTTGATGAATCATTGGCTTTGCTGTCAAAAATGGAAGAAAATGGTTGCATTCCAAACGCCGTAACTTATGAAATAATCATCCGTTCTCTGTTTGATAACGATGAAAATGATAAGGCGGAGAAACTTCTTCGTGAAATGATTACGAGAAGCCTATTGTAA

Protein sequence

>MsG0680035220.01.T01

MFHSSSSMLPRYYVVPVSLFVPKKFHSFQFLRNTHFKSIPYSSTSSTFHNNNDVVDDAVSLFNRLLRQNPTPPTIEFNKILGSLVKSKHYHTVLSLYQNMEYRGIKPNLVNCNILINSFCQLGLIPFAFSVLTKILKNGYEPDTITLTTFIKGLCLKGQIHQALHFHDKVIAMGFHLDQVSYGTLINGLCKVGETRAALELLRRVDGKLVQLNAVMYNTVIDGMCKDKHVNDAFDLYSEMVAKRISPNVVTYSALISGFCIVGKLRDAIDFFNKMIFENINPNVYTFTILVDGFCKEGRVKEAKIVLAMMMKQGIKPDVVTYNSLMDGYCLVKEVNKAKSIFNTMSQRGMNPDIHSYNIMINGFCKIKMTDEAMNLFEEMHCRKLIPDVVTYNSLIDGLSKSGKISNALKLVDEMHDRGQPPDIITYNSILDALCKNHQVDKAIAILTKLKDQGIRPNMYTYTILIDGLCKGGRLKDARNIFEDLLVKGYNVTVNTYTVMIQGFCSHGLFDESLALLSKMEENGCIPNAVTYEIIIRSLFDNDENDKAEKLLREMITRSLL*