Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030695.01.T01 | XP_003618437.1 | 93.678 | 522 | 31 | 1 | 1 | 522 | 1 | 520 | 0 | 936 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030695.01.T01 | Q9FLD5 | 62.391 | 460 | 163 | 5 | 5 | 457 | 4 | 460 | 0 | 584 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030695.01.T01 | G7KK16 | 93.678 | 522 | 31 | 1 | 1 | 522 | 1 | 520 | 0.0 | 936 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000653.01 | MsG0680030695.01 | 0.808108 | 3.641994e-50 | 2.064345e-47 |
| MsG0180001122.01 | MsG0680030695.01 | 0.841205 | 5.471016e-58 | 7.980164e-55 |
| MsG0180001142.01 | MsG0680030695.01 | 0.807929 | 3.976277e-50 | 2.243106e-47 |
| MsG0180001271.01 | MsG0680030695.01 | 0.808154 | 3.561182e-50 | 2.021104e-47 |
| MsG0180005279.01 | MsG0680030695.01 | 0.820563 | 6.420076e-53 | 5.097421e-50 |
| MsG0380013436.01 | MsG0680030695.01 | 0.803104 | 4.088331e-49 | 2.036804e-46 |
| MsG0480022120.01 | MsG0680030695.01 | 0.800786 | 1.224022e-48 | 5.749689e-46 |
| MsG0480023898.01 | MsG0680030695.01 | 0.864352 | 1.293496e-64 | 4.048909e-61 |
| MsG0480023900.01 | MsG0680030695.01 | 0.883184 | 5.559575e-71 | 3.488120e-67 |
| MsG0480023903.01 | MsG0680030695.01 | 0.819177 | 1.332077e-52 | 1.017631e-49 |
| MsG0580024853.01 | MsG0680030695.01 | 0.853668 | 2.052752e-61 | 4.469303e-58 |
| MsG0580025739.01 | MsG0680030695.01 | 0.801914 | 7.189799e-49 | 3.475259e-46 |
| MsG0380015005.01 | MsG0680030695.01 | 0.820280 | 7.456894e-53 | 5.874455e-50 |
| MsG0380015852.01 | MsG0680030695.01 | 0.834212 | 3.415958e-56 | 4.026113e-53 |
| MsG0380015877.01 | MsG0680030695.01 | 0.860076 | 2.657406e-63 | 7.175308e-60 |
| MsG0380015878.01 | MsG0680030695.01 | 0.838503 | 2.766973e-57 | 3.713269e-54 |
| MsG0380016061.01 | MsG0680030695.01 | 0.824295 | 8.723040e-54 | 7.694305e-51 |
| MsG0380016708.01 | MsG0680030695.01 | 0.864115 | 1.533475e-64 | 4.759984e-61 |
| MsG0580027832.01 | MsG0680030695.01 | 0.802828 | 4.661253e-49 | 2.306328e-46 |
| MsG0680030629.01 | MsG0680030695.01 | 0.848038 | 7.905188e-60 | 1.431980e-56 |
| MsG0680030694.01 | MsG0680030695.01 | 0.916828 | 1.113566e-85 | 3.118055e-81 |
| MsG0680030695.01 | MsG0680030696.01 | 0.854037 | 1.607160e-61 | 3.542865e-58 |
| MsG0680030695.01 | MsG0680035460.01 | 0.850963 | 1.208282e-60 | 2.408012e-57 |
| MsG0680030695.01 | MsG0780038113.01 | 0.803537 | 3.324703e-49 | 1.675073e-46 |
| MsG0680030695.01 | MsG0780040679.01 | 0.806389 | 8.422299e-50 | 4.565630e-47 |
| MsG0680030695.01 | MsG0780041485.01 | 0.831211 | 1.898432e-55 | 2.046217e-52 |
| MsG0680030695.01 | MsG0780041753.01 | 0.825369 | 4.866502e-54 | 4.426953e-51 |
| MsG0680030695.01 | MsG0780041777.01 | 0.861540 | 9.549149e-64 | 2.711606e-60 |
| MsG0680030695.01 | MsG0880045590.01 | 0.870171 | 1.785402e-66 | 6.878923e-63 |
| MsG0280007481.01 | MsG0680030695.01 | 0.807650 | 4.556187e-50 | 2.551701e-47 |
| MsG0280008073.01 | MsG0680030695.01 | 0.820457 | 6.789258e-53 | 5.375102e-50 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030695.01.T01 | MTR_6g009520 | 93.678 | 522 | 31 | 1 | 1 | 522 | 1 | 520 | 0.0 | 936 |
| MsG0680030695.01.T01 | MTR_6g009540 | 86.538 | 520 | 68 | 1 | 3 | 522 | 1 | 518 | 0.0 | 862 |
| MsG0680030695.01.T01 | MTR_6g009720 | 75.053 | 473 | 100 | 4 | 1 | 460 | 1 | 468 | 0.0 | 710 |
| MsG0680030695.01.T01 | MTR_6g009553 | 76.159 | 453 | 89 | 3 | 25 | 462 | 1 | 449 | 0.0 | 693 |
| MsG0680030695.01.T01 | MTR_6g009770 | 72.269 | 476 | 90 | 5 | 1 | 462 | 1 | 448 | 0.0 | 676 |
| MsG0680030695.01.T01 | MTR_6g042290 | 66.797 | 512 | 135 | 6 | 12 | 522 | 21 | 498 | 0.0 | 641 |
| MsG0680030695.01.T01 | MTR_7g008040 | 67.603 | 463 | 141 | 5 | 5 | 461 | 1 | 460 | 0.0 | 640 |
| MsG0680030695.01.T01 | MTR_6g009510 | 66.206 | 506 | 93 | 6 | 11 | 515 | 20 | 448 | 0.0 | 595 |
| MsG0680030695.01.T01 | MTR_6g009660 | 62.764 | 521 | 121 | 11 | 3 | 512 | 1 | 459 | 0.0 | 584 |
| MsG0680030695.01.T01 | MTR_4g107370 | 60.421 | 475 | 171 | 7 | 7 | 469 | 8 | 477 | 0.0 | 583 |
| MsG0680030695.01.T01 | MTR_1g022250 | 59.499 | 479 | 175 | 6 | 3 | 477 | 1 | 464 | 0.0 | 576 |
| MsG0680030695.01.T01 | MTR_2g030420 | 57.627 | 472 | 183 | 5 | 7 | 478 | 8 | 462 | 0.0 | 556 |
| MsG0680030695.01.T01 | MTR_1g022245 | 52.586 | 464 | 204 | 5 | 7 | 460 | 3 | 460 | 3.17e-172 | 496 |
| MsG0680030695.01.T01 | MTR_4g128260 | 43.038 | 474 | 253 | 6 | 7 | 472 | 3 | 467 | 2.04e-125 | 377 |
| MsG0680030695.01.T01 | MTR_5g020980 | 37.451 | 510 | 277 | 8 | 8 | 505 | 13 | 492 | 3.76e-115 | 350 |
| MsG0680030695.01.T01 | MTR_2g076970 | 39.157 | 498 | 252 | 11 | 12 | 503 | 7 | 459 | 4.74e-109 | 333 |
| MsG0680030695.01.T01 | MTR_2g076710 | 40.764 | 471 | 227 | 14 | 14 | 470 | 14 | 446 | 1.46e-107 | 328 |
| MsG0680030695.01.T01 | MTR_2g076920 | 39.139 | 511 | 246 | 14 | 12 | 514 | 13 | 466 | 3.28e-106 | 326 |
| MsG0680030695.01.T01 | MTR_2g076690 | 40.959 | 459 | 225 | 11 | 29 | 482 | 39 | 456 | 1.85e-105 | 323 |
| MsG0680030695.01.T01 | MTR_2g061820 | 39.503 | 443 | 242 | 9 | 5 | 441 | 4 | 426 | 6.24e-105 | 324 |
| MsG0680030695.01.T01 | MTR_2g076840 | 40.899 | 467 | 233 | 10 | 12 | 472 | 7 | 436 | 1.92e-104 | 329 |
| MsG0680030695.01.T01 | MTR_2g076840 | 49.474 | 285 | 112 | 6 | 180 | 462 | 450 | 704 | 2.44e-85 | 279 |
| MsG0680030695.01.T01 | MTR_8g076920 | 37.866 | 478 | 266 | 9 | 8 | 481 | 23 | 473 | 3.35e-103 | 319 |
| MsG0680030695.01.T01 | MTR_2g076900 | 38.972 | 467 | 249 | 10 | 12 | 472 | 7 | 443 | 1.62e-101 | 313 |
| MsG0680030695.01.T01 | MTR_2g076990 | 41.889 | 413 | 200 | 9 | 65 | 469 | 1 | 381 | 4.69e-101 | 311 |
| MsG0680030695.01.T01 | MTR_5g020990 | 36.983 | 484 | 270 | 12 | 3 | 472 | 19 | 481 | 7.45e-92 | 289 |
| MsG0680030695.01.T01 | MTR_3g103150 | 43.854 | 301 | 140 | 4 | 162 | 461 | 4 | 276 | 1.70e-81 | 256 |
| MsG0680030695.01.T01 | MTR_3g092060 | 30.172 | 464 | 265 | 11 | 8 | 462 | 2 | 415 | 1.37e-65 | 221 |
| MsG0680030695.01.T01 | MTR_7g117950 | 33.854 | 384 | 194 | 10 | 63 | 441 | 59 | 387 | 5.83e-65 | 218 |
| MsG0680030695.01.T01 | MTR_5g021020 | 37.855 | 317 | 150 | 7 | 146 | 461 | 135 | 405 | 5.59e-57 | 196 |
| MsG0680030695.01.T01 | MTR_8g027200 | 27.740 | 447 | 245 | 13 | 40 | 480 | 35 | 409 | 4.19e-50 | 178 |
| MsG0680030695.01.T01 | MTR_1g011680 | 60.741 | 135 | 48 | 3 | 328 | 460 | 2 | 133 | 5.48e-42 | 148 |
| MsG0680030695.01.T01 | MTR_5g021000 | 50.926 | 108 | 49 | 2 | 355 | 461 | 1 | 105 | 1.11e-29 | 114 |
| MsG0680030695.01.T01 | MTR_5g020720 | 40.331 | 181 | 88 | 5 | 268 | 447 | 25 | 186 | 2.60e-27 | 108 |
| MsG0680030695.01.T01 | MTR_1g113750 | 45.113 | 133 | 50 | 5 | 388 | 519 | 16 | 126 | 2.25e-20 | 87.4 |
| MsG0680030695.01.T01 | MTR_5g021010 | 47.253 | 91 | 44 | 2 | 372 | 461 | 34 | 121 | 1.15e-16 | 76.6 |
| MsG0680030695.01.T01 | MTR_7g096060 | 30.270 | 185 | 98 | 7 | 218 | 394 | 250 | 411 | 2.31e-12 | 70.1 |
| MsG0680030695.01.T01 | MTR_6g059690 | 27.907 | 301 | 165 | 14 | 205 | 480 | 194 | 467 | 2.22e-11 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030695.01.T01 | AT5G40010 | 62.391 | 460 | 163 | 5 | 5 | 457 | 4 | 460 | 0.0 | 584 |
| MsG0680030695.01.T01 | AT3G28580 | 61.388 | 461 | 161 | 8 | 5 | 457 | 4 | 455 | 0.0 | 551 |
| MsG0680030695.01.T01 | AT3G28510 | 59.002 | 461 | 187 | 2 | 6 | 466 | 5 | 463 | 0.0 | 534 |
| MsG0680030695.01.T01 | AT3G28580 | 58.079 | 458 | 159 | 7 | 5 | 457 | 4 | 433 | 7.58e-177 | 506 |
| MsG0680030695.01.T01 | AT3G28540 | 56.863 | 459 | 185 | 6 | 6 | 459 | 5 | 455 | 6.63e-171 | 493 |
| MsG0680030695.01.T01 | AT3G28540 | 56.863 | 459 | 185 | 6 | 6 | 459 | 5 | 455 | 8.92e-171 | 492 |
| MsG0680030695.01.T01 | AT3G28610 | 50.936 | 481 | 177 | 10 | 10 | 465 | 4 | 450 | 6.34e-165 | 476 |
| MsG0680030695.01.T01 | AT3G28600 | 50.522 | 479 | 194 | 8 | 1 | 465 | 1 | 450 | 2.59e-162 | 469 |
| MsG0680030695.01.T01 | AT5G40000 | 48.632 | 475 | 201 | 8 | 3 | 461 | 2 | 449 | 2.24e-154 | 449 |
| MsG0680030695.01.T01 | AT3G28520 | 55.301 | 481 | 192 | 9 | 17 | 487 | 2 | 469 | 1.52e-153 | 447 |
| MsG0680030695.01.T01 | AT3G28570 | 47.312 | 465 | 194 | 8 | 10 | 461 | 14 | 440 | 5.79e-145 | 424 |
| MsG0680030695.01.T01 | AT3G28570 | 47.312 | 465 | 194 | 8 | 10 | 461 | 7 | 433 | 7.90e-145 | 424 |
| MsG0680030695.01.T01 | AT3G28600 | 47.599 | 479 | 169 | 8 | 1 | 465 | 1 | 411 | 1.60e-143 | 420 |
| MsG0680030695.01.T01 | AT5G57480 | 39.096 | 509 | 286 | 8 | 7 | 505 | 3 | 497 | 2.15e-123 | 372 |
| MsG0680030695.01.T01 | AT4G25835 | 40.842 | 475 | 268 | 7 | 7 | 477 | 3 | 468 | 2.97e-122 | 368 |
| MsG0680030695.01.T01 | AT4G30250 | 40.552 | 471 | 258 | 8 | 3 | 461 | 1 | 461 | 1.66e-114 | 349 |
| MsG0680030695.01.T01 | AT4G30250 | 40.512 | 469 | 257 | 8 | 5 | 461 | 1 | 459 | 5.81e-114 | 347 |
| MsG0680030695.01.T01 | AT3G50940 | 39.286 | 448 | 235 | 8 | 29 | 471 | 36 | 451 | 1.82e-108 | 331 |
| MsG0680030695.01.T01 | AT3G50930 | 38.124 | 501 | 272 | 11 | 20 | 513 | 73 | 542 | 4.31e-108 | 334 |
| MsG0680030695.01.T01 | AT2G18193 | 39.212 | 482 | 254 | 10 | 13 | 491 | 19 | 464 | 1.45e-104 | 322 |
| MsG0680030695.01.T01 | AT5G17760 | 40.444 | 450 | 214 | 9 | 68 | 492 | 72 | 492 | 7.49e-103 | 318 |
| MsG0680030695.01.T01 | AT4G05380 | 38.994 | 477 | 259 | 11 | 4 | 475 | 11 | 460 | 6.73e-102 | 314 |
| MsG0680030695.01.T01 | AT5G17740 | 36.842 | 494 | 273 | 11 | 6 | 485 | 11 | 479 | 2.10e-101 | 315 |
| MsG0680030695.01.T01 | AT5G17730 | 36.585 | 492 | 265 | 12 | 5 | 482 | 10 | 468 | 5.40e-101 | 312 |
| MsG0680030695.01.T01 | AT2G18190 | 37.660 | 470 | 257 | 10 | 8 | 469 | 15 | 456 | 1.49e-96 | 301 |
| MsG0680030695.01.T01 | AT1G43910 | 38.266 | 473 | 262 | 10 | 4 | 471 | 10 | 457 | 1.19e-95 | 299 |
| MsG0680030695.01.T01 | AT5G17750 | 34.965 | 429 | 218 | 10 | 8 | 426 | 13 | 390 | 1.13e-83 | 265 |
| MsG0680030695.01.T01 | AT2G46620 | 30.952 | 462 | 268 | 8 | 5 | 461 | 1 | 416 | 3.85e-69 | 230 |
| MsG0680030695.01.T01 | AT5G17760 | 37.786 | 262 | 137 | 4 | 68 | 314 | 72 | 322 | 1.11e-52 | 182 |
| MsG0680030695.01.T01 | AT3G28560 | 42.593 | 216 | 114 | 4 | 6 | 219 | 5 | 212 | 1.80e-47 | 166 |
| MsG0680030695.01.T01 | AT3G29800 | 32.500 | 320 | 166 | 7 | 143 | 458 | 104 | 377 | 4.68e-46 | 167 |
| MsG0680030695.01.T01 | AT4G05340 | 52.083 | 96 | 40 | 1 | 317 | 412 | 4 | 93 | 5.55e-24 | 96.7 |
| MsG0680030695.01.T01 | AT1G09100 | 33.898 | 177 | 84 | 6 | 234 | 401 | 193 | 345 | 1.23e-13 | 73.2 |
| MsG0680030695.01.T01 | AT3G05530 | 33.333 | 177 | 85 | 6 | 234 | 401 | 194 | 346 | 5.06e-13 | 71.6 |
| MsG0680030695.01.T01 | AT3G02450 | 25.890 | 309 | 175 | 11 | 224 | 520 | 346 | 612 | 3.48e-12 | 69.3 |
Find 76 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGTAAGGTAACTCTTTC+TGG | 0.086559 | 6:-7295137 | MsG0680030695.01.T01:CDS |
| AAACGTGGTTATCTACTTTA+TGG | 0.185533 | 6:-7295431 | MsG0680030695.01.T01:CDS |
| CAAAGTATGAAGATGGGAAT+TGG | 0.285135 | 6:-7296169 | None:intergenic |
| ATGGTAGACTTTCCAGTTCC+TGG | 0.297845 | 6:+7295404 | None:intergenic |
| TTTGAGCATCCTGCAAGATT+TGG | 0.298067 | 6:-7295554 | MsG0680030695.01.T01:CDS |
| TCTTGAGATTGCAAAGAAAA+AGG | 0.305733 | 6:-7294787 | MsG0680030695.01.T01:CDS |
| TGAGGAGGAAGCAAGGAAAA+AGG | 0.320155 | 6:-7294763 | MsG0680030695.01.T01:CDS |
| ATAAAGTTTCATGAGTTATC+AGG | 0.325799 | 6:-7296001 | MsG0680030695.01.T01:CDS |
| AAAGTATGAAGATGGGAATT+GGG | 0.332092 | 6:-7296168 | None:intergenic |
| CCAACAACCCTAGTAGTAAT+TGG | 0.339715 | 6:-7295613 | MsG0680030695.01.T01:CDS |
| AACTTGATCCTGCTCTTATA+AGG | 0.349513 | 6:-7295034 | MsG0680030695.01.T01:CDS |
| AATGGATAAGCATATAGAAA+TGG | 0.368048 | 6:-7295003 | MsG0680030695.01.T01:CDS |
| TTGTAGCTATCAAGCTTTCA+AGG | 0.388003 | 6:-7294976 | MsG0680030695.01.T01:CDS |
| TCCAATTATTGAAAAGTTGT+TGG | 0.390561 | 6:-7294907 | MsG0680030695.01.T01:CDS |
| CCAATTATTGAAAAGTTGTT+GGG | 0.400795 | 6:-7294906 | MsG0680030695.01.T01:CDS |
| GCTAGGGTTCCAAATCTTGC+AGG | 0.402544 | 6:+7295545 | None:intergenic |
| AAAAGGATGAGGAGGAAGCA+AGG | 0.404612 | 6:-7294770 | MsG0680030695.01.T01:CDS |
| AGTTCAATGGAGTCAAAGTT+TGG | 0.406209 | 6:-7295823 | MsG0680030695.01.T01:CDS |
| TTCTTTCTTCTTCCTTTGCC+CGG | 0.416876 | 6:+7295229 | None:intergenic |
| CTTTATGGTCCACCAGGAAC+TGG | 0.417256 | 6:-7295416 | MsG0680030695.01.T01:CDS |
| AAATCTTCAGTGATTGACTT+TGG | 0.418717 | 6:+7294840 | None:intergenic |
| AGAAAATATGCACGAAGATA+AGG | 0.421661 | 6:+7296070 | None:intergenic |
| TGAAACTTTATGTTGATGTA+TGG | 0.421952 | 6:+7296013 | None:intergenic |
| AACATTTCAAAGTATGAAGA+TGG | 0.422358 | 6:-7296176 | None:intergenic |
| ATGGATAAGCATATAGAAAT+GGG | 0.422830 | 6:-7295002 | MsG0680030695.01.T01:CDS |
| CAACACATGTTGAATGTAAG+AGG | 0.425064 | 6:+7295685 | None:intergenic |
| TACATCCACCAATTACTACT+AGG | 0.426977 | 6:+7295605 | None:intergenic |
| CCAATTACTACTAGGGTTGT+TGG | 0.427886 | 6:+7295613 | None:intergenic |
| CTCTTACATTCAACATGTGT+TGG | 0.428090 | 6:-7295684 | MsG0680030695.01.T01:CDS |
| AAAGAGTATTATGCTAAAGT+TGG | 0.429304 | 6:-7295464 | MsG0680030695.01.T01:CDS |
| TCTTGAGTTAACAACTGTTA+AGG | 0.446059 | 6:-7295339 | MsG0680030695.01.T01:CDS |
| AGTTTGCACTAAGATATGTT+TGG | 0.446804 | 6:+7295942 | None:intergenic |
| ATTCAACATGTGTTGGATGA+AGG | 0.453587 | 6:-7295677 | MsG0680030695.01.T01:CDS |
| GATCTTCTTAAGTTCAAGAA+AGG | 0.458025 | 6:-7295488 | MsG0680030695.01.T01:CDS |
| AGATGGGAATTGGGGAACTA+TGG | 0.460698 | 6:-7296159 | MsG0680030695.01.T01:CDS |
| ATGCTAAAGTTGGAAAAGCT+TGG | 0.482796 | 6:-7295454 | MsG0680030695.01.T01:CDS |
| TTCAACATGTGTTGGATGAA+GGG | 0.485677 | 6:-7295676 | MsG0680030695.01.T01:CDS |
| CTTCCCCTCCTTATAAGAGC+AGG | 0.487071 | 6:+7295026 | None:intergenic |
| ATTGACTCAAGAGGAAGAAA+AGG | 0.488311 | 6:-7294688 | MsG0680030695.01.T01:CDS |
| AAGCTATTATGAGCAAGAAT+AGG | 0.501969 | 6:-7295652 | MsG0680030695.01.T01:CDS |
| CTCTCGATCTCACCGGGCAA+AGG | 0.502496 | 6:-7295241 | MsG0680030695.01.T01:CDS |
| GATTGTTCTCTCGATCTCAC+CGG | 0.518830 | 6:-7295248 | MsG0680030695.01.T01:CDS |
| ATTTGCTATAGCTGATATCA+TGG | 0.523985 | 6:+7295385 | None:intergenic |
| AAATTCTCAGCAACATCAGC+AGG | 0.526229 | 6:+7294867 | None:intergenic |
| GAAATCATAGATGAGTTCAA+TGG | 0.530945 | 6:-7295836 | MsG0680030695.01.T01:CDS |
| TCAAGAGGAAGAAAAGGTTA+AGG | 0.538611 | 6:-7294682 | MsG0680030695.01.T01:CDS |
| ACATCCACCAATTACTACTA+GGG | 0.539649 | 6:+7295606 | None:intergenic |
| TCATCCATGCTTAGAACAAG+TGG | 0.540829 | 6:+7295866 | None:intergenic |
| TCTTATAAGGAGGGGAAGAA+TGG | 0.542051 | 6:-7295021 | MsG0680030695.01.T01:CDS |
| ATTGTTCTCTCGATCTCACC+GGG | 0.546404 | 6:-7295247 | MsG0680030695.01.T01:CDS |
| GATGGAATTTGGTCAGCTTG+TGG | 0.549571 | 6:-7295098 | MsG0680030695.01.T01:CDS |
| GTTGGAAAAGCTTGGAAACG+TGG | 0.566291 | 6:-7295446 | MsG0680030695.01.T01:CDS |
| CAACTAGGTTCAATCACGGC+TGG | 0.570804 | 6:-7296133 | MsG0680030695.01.T01:CDS |
| CATCCATGCTTAGAACAAGT+GGG | 0.570942 | 6:+7295867 | None:intergenic |
| TGATGAGAATGATGAAAAGA+AGG | 0.571153 | 6:-7295195 | MsG0680030695.01.T01:CDS |
| AAATATGCACGAAGATAAGG+TGG | 0.571817 | 6:+7296073 | None:intergenic |
| AACCCCACTTGTTCTAAGCA+TGG | 0.576665 | 6:-7295870 | MsG0680030695.01.T01:CDS |
| AAGTATGAAGATGGGAATTG+GGG | 0.585836 | 6:-7296167 | None:intergenic |
| TGATCCTGCTCTTATAAGGA+GGG | 0.590776 | 6:-7295030 | MsG0680030695.01.T01:CDS |
| TCAATGGAGTCAAAGTTTGG+TGG | 0.596166 | 6:-7295820 | MsG0680030695.01.T01:CDS |
| ACATTTCAAAGTATGAAGAT+GGG | 0.598757 | 6:-7296175 | None:intergenic |
| TTGATCCTGCTCTTATAAGG+AGG | 0.600009 | 6:-7295031 | MsG0680030695.01.T01:CDS |
| GGATGAGGAAGCTCGGCTCA+AGG | 0.601592 | 6:-7294736 | MsG0680030695.01.T01:CDS |
| GTAGACTTTCCAGTTCCTGG+TGG | 0.611663 | 6:+7295407 | None:intergenic |
| AGGTAGAGGATGAGGAAGCT+CGG | 0.611983 | 6:-7294743 | MsG0680030695.01.T01:CDS |
| TATCTACTTTATGGTCCACC+AGG | 0.617929 | 6:-7295422 | MsG0680030695.01.T01:CDS |
| GGGGAACTATGGTCACAACT+AGG | 0.620126 | 6:-7296148 | MsG0680030695.01.T01:CDS |
| AAGATTTGGAACCCTAGCTA+TGG | 0.624596 | 6:-7295540 | MsG0680030695.01.T01:CDS |
| ACAACCCTAGTAGTAATTGG+TGG | 0.629329 | 6:-7295610 | MsG0680030695.01.T01:CDS |
| ATCCATGCTTAGAACAAGTG+GGG | 0.634090 | 6:+7295868 | None:intergenic |
| GAGAATGGTGTCAAAGACAA+TGG | 0.641961 | 6:-7294630 | MsG0680030695.01.T01:CDS |
| GTCACAACTAGGTTCAATCA+CGG | 0.654145 | 6:-7296137 | MsG0680030695.01.T01:CDS |
| GATCCTGCTCTTATAAGGAG+GGG | 0.654308 | 6:-7295029 | MsG0680030695.01.T01:CDS |
| GGATGTATAAAAGCACAAAG+TGG | 0.671130 | 6:-7295589 | MsG0680030695.01.T01:CDS |
| AATATGCACGAAGATAAGGT+GGG | 0.699030 | 6:+7296074 | None:intergenic |
| GGTCAGCTTGTGGATCAGAG+AGG | 0.714357 | 6:-7295088 | MsG0680030695.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTCACAACAAATTTTG+TGG | - | Chr6:7295720-7295739 | MsG0680030695.01.T01:CDS | 20.0% |
| !!! | TGAATTTTATTGATGGAATT+TGG | - | Chr6:7295671-7295690 | MsG0680030695.01.T01:CDS | 20.0% |
| ! | AAAATTAGAGGAAAAAGTGA+AGG | - | Chr6:7296128-7296147 | MsG0680030695.01.T01:CDS | 25.0% |
| ! | AATGGATAAGCATATAGAAA+TGG | - | Chr6:7295777-7295796 | MsG0680030695.01.T01:CDS | 25.0% |
| ! | AGAAGAGAAAAATGAAAGTA+AGG | - | Chr6:7295630-7295649 | MsG0680030695.01.T01:CDS | 25.0% |
| ! | ATAAAGTTTCATGAGTTATC+AGG | - | Chr6:7294779-7294798 | MsG0680030695.01.T01:CDS | 25.0% |
| ! | ATGGATAAGCATATAGAAAT+GGG | - | Chr6:7295778-7295797 | MsG0680030695.01.T01:CDS | 25.0% |
| ! | CCAATTATTGAAAAGTTGTT+GGG | - | Chr6:7295874-7295893 | MsG0680030695.01.T01:CDS | 25.0% |
| ! | TCCAATTATTGAAAAGTTGT+TGG | - | Chr6:7295873-7295892 | MsG0680030695.01.T01:CDS | 25.0% |
| ! | TGAAACTTTATGTTGATGTA+TGG | + | Chr6:7294770-7294789 | None:intergenic | 25.0% |
| !! | AGAAAATGATTGTGATTTTG+AGG | + | Chr6:7295002-7295021 | None:intergenic | 25.0% |
| !!! | AAAGAGTATTATGCTAAAGT+TGG | - | Chr6:7295316-7295335 | MsG0680030695.01.T01:CDS | 25.0% |
| !!! | AATCTTTTTTCATCTGAAGT+AGG | + | Chr6:7295031-7295050 | None:intergenic | 25.0% |
| !!! | TAAGGTTGATGAAAAATTAG+AGG | - | Chr6:7296116-7296135 | MsG0680030695.01.T01:CDS | 25.0% |
| !!! | TCTTTTTCAGCTTTCTTAAT+AGG | + | Chr6:7295613-7295632 | None:intergenic | 25.0% |
| AAACGTGGTTATCTACTTTA+TGG | - | Chr6:7295349-7295368 | MsG0680030695.01.T01:CDS | 30.0% | |
| AAGCTATTATGAGCAAGAAT+AGG | - | Chr6:7295128-7295147 | MsG0680030695.01.T01:CDS | 30.0% | |
| AGAAAATATGCACGAAGATA+AGG | + | Chr6:7294713-7294732 | None:intergenic | 30.0% | |
| AGTTTGCACTAAGATATGTT+TGG | + | Chr6:7294841-7294860 | None:intergenic | 30.0% | |
| GAAATCATAGATGAGTTCAA+TGG | - | Chr6:7294944-7294963 | MsG0680030695.01.T01:CDS | 30.0% | |
| GATCTTCTTAAGTTCAAGAA+AGG | - | Chr6:7295292-7295311 | MsG0680030695.01.T01:CDS | 30.0% | |
| TCTTGAGATTGCAAAGAAAA+AGG | - | Chr6:7295993-7296012 | MsG0680030695.01.T01:CDS | 30.0% | |
| TCTTGAGTTAACAACTGTTA+AGG | - | Chr6:7295441-7295460 | MsG0680030695.01.T01:CDS | 30.0% | |
| TGATGAGAATGATGAAAAGA+AGG | - | Chr6:7295585-7295604 | MsG0680030695.01.T01:CDS | 30.0% | |
| TGGGAAAAATTTGTCAAACA+TGG | + | Chr6:7294690-7294709 | None:intergenic | 30.0% | |
| ! | AAATCTTCAGTGATTGACTT+TGG | + | Chr6:7295943-7295962 | None:intergenic | 30.0% |
| ! | AGAATCTCTTCTTTCTTTTC+TGG | + | Chr6:7295268-7295287 | None:intergenic | 30.0% |
| ! | CCCAACAACTTTTCAATAAT+TGG | + | Chr6:7295877-7295896 | None:intergenic | 30.0% |
| ! | GAAACAAAAATTGACTCAAG+AGG | - | Chr6:7296083-7296102 | MsG0680030695.01.T01:CDS | 30.0% |
| !! | AAATGATTGTGATTTTGAGG+TGG | + | Chr6:7294999-7295018 | None:intergenic | 30.0% |
| !! | ATGCTTTTTGTGGAAAGTTA+GGG | + | Chr6:7295059-7295078 | None:intergenic | 30.0% |
| !! | ATTTGCTATAGCTGATATCA+TGG | + | Chr6:7295398-7295417 | None:intergenic | 30.0% |
| !!! | GGTTTGCTGAATTTTATTGA+TGG | - | Chr6:7295664-7295683 | MsG0680030695.01.T01:CDS | 30.0% |
| AAATATGCACGAAGATAAGG+TGG | + | Chr6:7294710-7294729 | None:intergenic | 35.0% | |
| AACTTGATCCTGCTCTTATA+AGG | - | Chr6:7295746-7295765 | MsG0680030695.01.T01:CDS | 35.0% | |
| AATATGCACGAAGATAAGGT+GGG | + | Chr6:7294709-7294728 | None:intergenic | 35.0% | |
| ACATCCACCAATTACTACTA+GGG | + | Chr6:7295177-7295196 | None:intergenic | 35.0% | |
| AGTTCAATGGAGTCAAAGTT+TGG | - | Chr6:7294957-7294976 | MsG0680030695.01.T01:CDS | 35.0% | |
| ATGCTAAAGTTGGAAAAGCT+TGG | - | Chr6:7295326-7295345 | MsG0680030695.01.T01:CDS | 35.0% | |
| ATTCAACATGTGTTGGATGA+AGG | - | Chr6:7295103-7295122 | MsG0680030695.01.T01:CDS | 35.0% | |
| CAACACATGTTGAATGTAAG+AGG | + | Chr6:7295098-7295117 | None:intergenic | 35.0% | |
| CTCTTACATTCAACATGTGT+TGG | - | Chr6:7295096-7295115 | MsG0680030695.01.T01:CDS | 35.0% | |
| GAAAGTAAGGTAACTCTTTC+TGG | - | Chr6:7295643-7295662 | MsG0680030695.01.T01:CDS | 35.0% | |
| GATTGCAAAGAAAAAGGATG+AGG | - | Chr6:7295999-7296018 | MsG0680030695.01.T01:CDS | 35.0% | |
| TACATCCACCAATTACTACT+AGG | + | Chr6:7295178-7295197 | None:intergenic | 35.0% | |
| TCAAGAGGAAGAAAAGGTTA+AGG | - | Chr6:7296098-7296117 | MsG0680030695.01.T01:CDS | 35.0% | |
| TTCAACATGTGTTGGATGAA+GGG | - | Chr6:7295104-7295123 | MsG0680030695.01.T01:CDS | 35.0% | |
| TTGTAGCTATCAAGCTTTCA+AGG | - | Chr6:7295804-7295823 | MsG0680030695.01.T01:CDS | 35.0% | |
| ! | ATTGACTCAAGAGGAAGAAA+AGG | - | Chr6:7296092-7296111 | MsG0680030695.01.T01:CDS | 35.0% |
| ! | GGATGTATAAAAGCACAAAG+TGG | - | Chr6:7295191-7295210 | MsG0680030695.01.T01:CDS | 35.0% |
| !! | GATGCTTTTTGTGGAAAGTT+AGG | + | Chr6:7295060-7295079 | None:intergenic | 35.0% |
| AAATTCTCAGCAACATCAGC+AGG | + | Chr6:7295916-7295935 | None:intergenic | 40.0% | |
| AAGGAAAAAGGTAGAGGATG+AGG | - | Chr6:7296029-7296048 | MsG0680030695.01.T01:CDS | 40.0% | |
| ACAACCCTAGTAGTAATTGG+TGG | - | Chr6:7295170-7295189 | MsG0680030695.01.T01:CDS | 40.0% | |
| ATCCATGCTTAGAACAAGTG+GGG | + | Chr6:7294915-7294934 | None:intergenic | 40.0% | |
| CATCCATGCTTAGAACAAGT+GGG | + | Chr6:7294916-7294935 | None:intergenic | 40.0% | |
| CCAACAACCCTAGTAGTAAT+TGG | - | Chr6:7295167-7295186 | MsG0680030695.01.T01:CDS | 40.0% | |
| CCAATTACTACTAGGGTTGT+TGG | + | Chr6:7295170-7295189 | None:intergenic | 40.0% | |
| GAGGAAAAAGTGAAGGAGAA+TGG | - | Chr6:7296135-7296154 | MsG0680030695.01.T01:CDS | 40.0% | |
| GTCACAACTAGGTTCAATCA+CGG | - | Chr6:7294643-7294662 | MsG0680030695.01.T01:CDS | 40.0% | |
| TATCTACTTTATGGTCCACC+AGG | - | Chr6:7295358-7295377 | MsG0680030695.01.T01:CDS | 40.0% | |
| TCAATGGAGTCAAAGTTTGG+TGG | - | Chr6:7294960-7294979 | MsG0680030695.01.T01:CDS | 40.0% | |
| TCATCCATGCTTAGAACAAG+TGG | + | Chr6:7294917-7294936 | None:intergenic | 40.0% | |
| TCTTATAAGGAGGGGAAGAA+TGG | - | Chr6:7295759-7295778 | MsG0680030695.01.T01:CDS | 40.0% | |
| TGATCCTGCTCTTATAAGGA+GGG | - | Chr6:7295750-7295769 | MsG0680030695.01.T01:CDS | 40.0% | |
| TGCAAAGAAAAAGGATGAGG+AGG | - | Chr6:7296002-7296021 | MsG0680030695.01.T01:CDS | 40.0% | |
| TTCTTTCTTCTTCCTTTGCC+CGG | + | Chr6:7295554-7295573 | None:intergenic | 40.0% | |
| TTGATCCTGCTCTTATAAGG+AGG | - | Chr6:7295749-7295768 | MsG0680030695.01.T01:CDS | 40.0% | |
| TTTGAGCATCCTGCAAGATT+TGG | - | Chr6:7295226-7295245 | MsG0680030695.01.T01:CDS | 40.0% | |
| ! | AAGATTTGGAACCCTAGCTA+TGG | - | Chr6:7295240-7295259 | MsG0680030695.01.T01:CDS | 40.0% |
| ! | GAGAATGGTGTCAAAGACAA+TGG | - | Chr6:7296150-7296169 | MsG0680030695.01.T01:CDS | 40.0% |
| !! | TGAGTTCACGATGCTTTTTG+TGG | + | Chr6:7295069-7295088 | None:intergenic | 40.0% |
| !!! | CTTTTCTGGTTCCATAGCTA+GGG | + | Chr6:7295254-7295273 | None:intergenic | 40.0% |
| !!! | TCTTTTCTGGTTCCATAGCT+AGG | + | Chr6:7295255-7295274 | None:intergenic | 40.0% |
| AAAAGGATGAGGAGGAAGCA+AGG | - | Chr6:7296010-7296029 | MsG0680030695.01.T01:CDS | 45.0% | |
| AGATGGGAATTGGGGAACTA+TGG | - | Chr6:7294621-7294640 | MsG0680030695.01.T01:CDS | 45.0% | |
| ATGGTAGACTTTCCAGTTCC+TGG | + | Chr6:7295379-7295398 | None:intergenic | 45.0% | |
| ATTGTTCTCTCGATCTCACC+GGG | - | Chr6:7295533-7295552 | MsG0680030695.01.T01:CDS | 45.0% | |
| GATCCTGCTCTTATAAGGAG+GGG | - | Chr6:7295751-7295770 | MsG0680030695.01.T01:CDS | 45.0% | |
| GATGGAATTTGGTCAGCTTG+TGG | - | Chr6:7295682-7295701 | MsG0680030695.01.T01:CDS | 45.0% | |
| GATTGTTCTCTCGATCTCAC+CGG | - | Chr6:7295532-7295551 | MsG0680030695.01.T01:CDS | 45.0% | |
| GGAAGCAAGGAAAAAGGTAG+AGG | - | Chr6:7296023-7296042 | MsG0680030695.01.T01:CDS | 45.0% | |
| GTTGGAAAAGCTTGGAAACG+TGG | - | Chr6:7295334-7295353 | MsG0680030695.01.T01:CDS | 45.0% | |
| TGAGGAGGAAGCAAGGAAAA+AGG | - | Chr6:7296017-7296036 | MsG0680030695.01.T01:CDS | 45.0% | |
| ! | AACCCCACTTGTTCTAAGCA+TGG | - | Chr6:7294910-7294929 | MsG0680030695.01.T01:CDS | 45.0% |
| AGGTAGAGGATGAGGAAGCT+CGG | - | Chr6:7296037-7296056 | MsG0680030695.01.T01:CDS | 50.0% | |
| CAACTAGGTTCAATCACGGC+TGG | - | Chr6:7294647-7294666 | MsG0680030695.01.T01:CDS | 50.0% | |
| CTTCCCCTCCTTATAAGAGC+AGG | + | Chr6:7295757-7295776 | None:intergenic | 50.0% | |
| CTTTATGGTCCACCAGGAAC+TGG | - | Chr6:7295364-7295383 | MsG0680030695.01.T01:CDS | 50.0% | |
| GCTAGGGTTCCAAATCTTGC+AGG | + | Chr6:7295238-7295257 | None:intergenic | 50.0% | |
| GGGGAACTATGGTCACAACT+AGG | - | Chr6:7294632-7294651 | MsG0680030695.01.T01:CDS | 50.0% | |
| GTAGACTTTCCAGTTCCTGG+TGG | + | Chr6:7295376-7295395 | None:intergenic | 50.0% | |
| GGTCAGCTTGTGGATCAGAG+AGG | - | Chr6:7295692-7295711 | MsG0680030695.01.T01:CDS | 55.0% | |
| CTCTCGATCTCACCGGGCAA+AGG | - | Chr6:7295539-7295558 | MsG0680030695.01.T01:CDS | 60.0% | |
| GGATGAGGAAGCTCGGCTCA+AGG | - | Chr6:7296044-7296063 | MsG0680030695.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 7294617 | 7296185 | 7294617 | ID=MsG0680030695.01;Name=MsG0680030695.01 |
| Chr6 | mRNA | 7294617 | 7296185 | 7294617 | ID=MsG0680030695.01.T01;Parent=MsG0680030695.01;Name=MsG0680030695.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|522 |
| Chr6 | exon | 7294617 | 7296185 | 7294617 | ID=MsG0680030695.01.T01:exon:3215;Parent=MsG0680030695.01.T01 |
| Chr6 | CDS | 7294617 | 7296185 | 7294617 | ID=MsG0680030695.01.T01:cds;Parent=MsG0680030695.01.T01 |
| Gene Sequence |
| Protein sequence |
