AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002571.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002571.01.T01 MTR_7g107690 96.543 376 13 0 1 376 1 376 0.0 758
MsG0180002571.01.T01 MTR_7g107710 68.182 374 117 2 8 380 6 378 0.0 540
MsG0180002571.01.T01 MTR_8g432640 49.730 370 186 0 8 377 6 375 1.73e-138 400
MsG0180002571.01.T01 MTR_5g033260 50.000 370 185 0 8 377 6 375 3.50e-136 394
MsG0180002571.01.T01 MTR_3g088925 50.134 373 186 0 8 380 7 379 7.29e-135 391
MsG0180002571.01.T01 MTR_3g088945 50.134 373 186 0 8 380 7 379 1.30e-134 390
MsG0180002571.01.T01 MTR_3g088955 49.330 373 189 0 8 380 7 379 5.44e-132 384
MsG0180002571.01.T01 MTR_4g051642 48.794 373 190 1 8 380 7 378 3.59e-129 377
MsG0180002571.01.T01 MTR_3g109520 46.933 375 193 2 9 378 40 413 8.75e-123 361
MsG0180002571.01.T01 MTR_6g086870 44.819 386 202 4 9 385 21 404 7.60e-120 353
MsG0180002571.01.T01 MTR_1g008380 47.185 373 190 3 9 376 43 413 1.19e-119 353
MsG0180002571.01.T01 MTR_1g069275 45.600 375 194 5 9 377 26 396 2.13e-118 350
MsG0180002571.01.T01 MTR_1g046230 44.415 376 200 4 9 377 25 398 1.13e-115 343
MsG0180002571.01.T01 MTR_7g099330 43.968 373 197 4 9 376 25 390 2.26e-115 342
MsG0180002571.01.T01 MTR_1g011630 43.307 381 205 4 9 384 17 391 5.36e-115 341
MsG0180002571.01.T01 MTR_7g099160 40.984 305 168 4 77 376 214 511 4.13e-78 250
MsG0180002571.01.T01 MTR_3g096030 40.331 181 84 1 4 184 2 158 9.12e-45 152
MsG0180002571.01.T01 MTR_1g041605 82.500 40 7 0 112 151 29 68 1.13e-16 74.3
MsG0180002571.01.T01 MTR_8g037910 25.106 235 142 5 7 214 3 230 3.66e-15 75.5
MsG0180002571.01.T01 MTR_8g037910 25.106 235 142 5 7 214 3 230 5.27e-15 75.5
MsG0180002571.01.T01 MTR_8g037910 25.106 235 142 5 7 214 3 230 1.12e-14 75.5
MsG0180002571.01.T01 MTR_8g037880 23.849 239 149 6 7 217 3 236 2.90e-14 74.3
MsG0180002571.01.T01 MTR_3g437580 25.101 247 138 6 4 214 9 244 6.84e-14 72.8
MsG0180002571.01.T01 MTR_3g437580 25.101 247 138 6 4 214 9 244 1.60e-13 72.0
MsG0180002571.01.T01 MTR_2g450310 23.016 378 227 15 1 318 1 374 6.98e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002571.01.T01 AT1G48910 47.967 369 192 0 8 376 5 373 6.41e-137 396
MsG0180002571.01.T01 AT1G21430 46.438 379 197 5 1 376 1 376 8.53e-124 363
MsG0180002571.01.T01 AT1G04610 46.419 377 192 5 9 377 38 412 1.85e-121 358
MsG0180002571.01.T01 AT4G13260 44.947 376 201 2 9 379 29 403 3.89e-119 352
MsG0180002571.01.T01 AT5G25620 46.400 375 191 4 9 376 33 404 3.85e-117 347
MsG0180002571.01.T01 AT5G25620 46.400 375 191 4 9 376 33 404 6.62e-117 347
MsG0180002571.01.T01 AT5G43890 44.267 375 200 5 9 376 26 398 6.12e-116 344
MsG0180002571.01.T01 AT4G32540 44.211 380 199 5 9 382 22 394 1.50e-115 343
MsG0180002571.01.T01 AT4G32540 44.619 381 198 5 9 382 22 396 2.29e-115 342
MsG0180002571.01.T01 AT1G04180 44.920 374 196 5 9 376 26 395 7.22e-115 341
MsG0180002571.01.T01 AT4G28720 43.617 376 202 5 9 376 26 399 2.15e-114 340
MsG0180002571.01.T01 AT5G11320 44.920 374 193 4 9 377 18 383 6.31e-113 336
MsG0180002571.01.T01 AT2G33230 44.297 377 199 5 9 377 33 406 1.66e-108 325
MsG0180002571.01.T01 AT5G11320 44.892 323 166 3 9 326 18 333 2.91e-96 291
MsG0180002571.01.T01 AT4G13260 46.099 282 147 1 9 285 29 310 3.11e-92 280
MsG0180002571.01.T01 AT4G13260 41.275 298 168 3 87 379 5 300 1.74e-78 244
MsG0180002571.01.T01 AT1G65860 27.542 236 135 4 5 213 10 236 5.88e-16 79.7
MsG0180002571.01.T01 AT1G62560 27.615 239 132 7 5 213 10 237 6.78e-16 79.0
MsG0180002571.01.T01 AT1G62560 27.966 236 129 7 8 213 13 237 8.16e-16 79.3
MsG0180002571.01.T01 AT1G12160 24.044 366 176 10 8 342 12 306 9.46e-14 72.8
MsG0180002571.01.T01 AT1G12140 28.054 221 146 5 49 258 74 292 1.62e-13 72.0
MsG0180002571.01.T01 AT1G12140 28.054 221 146 5 49 258 74 292 1.62e-13 72.0
MsG0180002571.01.T01 AT5G07800 27.413 259 139 8 5 226 13 259 3.79e-13 70.9
MsG0180002571.01.T01 AT1G19250 24.896 241 136 9 5 205 10 245 2.03e-12 68.9
MsG0180002571.01.T01 AT5G61290 26.971 241 136 6 1 205 9 245 3.23e-12 68.2
MsG0180002571.01.T01 AT1G62540 27.917 240 129 6 5 213 10 236 8.84e-12 66.6
MsG0180002571.01.T01 AT1G62540 27.848 237 133 5 5 213 10 236 1.01e-11 66.6
MsG0180002571.01.T01 AT1G62620 23.605 233 138 6 8 205 13 240 1.11e-11 66.2
MsG0180002571.01.T01 AT1G62540 27.848 237 133 5 5 213 10 236 1.15e-11 66.6
MsG0180002571.01.T01 AT1G12200 29.592 196 110 7 80 258 113 297 7.12e-11 63.5
MsG0180002571.01.T01 AT1G12200 32.857 140 78 5 80 213 113 242 8.66e-11 63.9

Find 83 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAAGGGGTATGCTGAATT+TGG 0.142332 1:+40562911 MsG0180002571.01.T01:CDS
CTCTTCAACCCTTCAATTTC+AGG 0.149369 1:-40562027 None:intergenic
TACGGGGATGTCACGAATTA+TGG 0.154022 1:+40563005 MsG0180002571.01.T01:CDS
GTTGTTGGGTCTGGGAATTC+TGG 0.176673 1:+40562120 MsG0180002571.01.T01:CDS
GTACTTGACCTTCATATAAA+AGG 0.212639 1:-40563051 None:intergenic
TCAAGGGGTATGCTGAATTT+GGG 0.240991 1:+40562912 MsG0180002571.01.T01:CDS
GTTGTTGGAGCAGGCCCTTC+TGG 0.254075 1:+40561616 MsG0180002571.01.T01:CDS
CATGTTCTTGTTGTTGGGTC+TGG 0.258715 1:+40562111 MsG0180002571.01.T01:CDS
GATGGTGATGCTTAGTAAAA+TGG 0.306091 1:+40562980 MsG0180002571.01.T01:CDS
AATTAAGTCTCGAGAGTTAA+AGG 0.307124 1:+40563112 MsG0180002571.01.T01:CDS
TCTCTTTCGAGGATTATATA+AGG 0.314818 1:-40561676 None:intergenic
ATGCTTAGTAAAATGGTTTA+CGG 0.339605 1:+40562987 MsG0180002571.01.T01:CDS
GAAGGTGAAGGCTAAGAATA+AGG 0.345400 1:+40561930 MsG0180002571.01.T01:CDS
AATGAAAGAGTGATAGTTGT+TGG 0.360593 1:+40561601 MsG0180002571.01.T01:CDS
GGGAAAGTGATTCATTCAAC+TGG 0.376100 1:+40562057 MsG0180002571.01.T01:CDS
GATGAACATGTTCTTGTTGT+TGG 0.376175 1:+40562105 MsG0180002571.01.T01:CDS
TGCTTAGTAAAATGGTTTAC+GGG 0.386664 1:+40562988 MsG0180002571.01.T01:CDS
ATCAACCCACAAGTGGCTTA+AGG 0.388610 1:+40564260 MsG0180002571.01.T01:CDS
AGCCAAGTTACCCTATTCAT+TGG 0.388713 1:+40564717 MsG0180002571.01.T01:CDS
ATGTTCTTGTTGTTGGGTCT+GGG 0.391709 1:+40562112 MsG0180002571.01.T01:CDS
CATTCAACTGGGTTTAAGAA+TGG 0.393718 1:+40562069 MsG0180002571.01.T01:CDS
ATGATGAAGGTTCTGAGAAA+TGG 0.397382 1:+40561908 MsG0180002571.01.T01:CDS
CCTTCAATTTCAGGTACAAA+AGG 0.400011 1:-40562018 None:intergenic
CTATCAAGGAGAGGTTTCTA+TGG 0.400558 1:+40564770 MsG0180002571.01.T01:CDS
ACGGGGATGTCACGAATTAT+GGG 0.428718 1:+40563006 MsG0180002571.01.T01:CDS
TATATTGTGTTGGACTATCA+AGG 0.438069 1:+40564756 MsG0180002571.01.T01:CDS
ATTCAACTGGGTTTAAGAAT+GGG 0.438492 1:+40562070 MsG0180002571.01.T01:CDS
CTTAAGCATTCTATTTCAGC+TGG 0.447488 1:-40564162 None:intergenic
TCAACTGTTCTGTGATAAAG+AGG 0.449237 1:-40561874 None:intergenic
ATGAATAGGGTAACTTGGCT+TGG 0.450552 1:-40564714 None:intergenic
AAGGTTCCAACGTCAATAAT+AGG 0.451735 1:-40563083 None:intergenic
TTCGAGGATTATATAAGGAA+TGG 0.455797 1:-40561671 None:intergenic
TTCCAATGAATAGGGTAACT+TGG 0.456789 1:-40564719 None:intergenic
TAAGGATACCGGTGAGGTTG+AGG 0.468786 1:+40561948 MsG0180002571.01.T01:CDS
TTCTGGCATGGAAATTGCAT+TGG 0.469456 1:+40562137 MsG0180002571.01.T01:CDS
ATGAACATGTTCTTGTTGTT+GGG 0.471420 1:+40562106 MsG0180002571.01.T01:CDS
TACTTGACCTTCATATAAAA+GGG 0.473078 1:-40563050 None:intergenic
GCAAACCTTAAGCCACTTGT+GGG 0.480163 1:-40564265 None:intergenic
TGAATAGGGTAACTTGGCTT+GGG 0.488661 1:-40564713 None:intergenic
GGATTATATAAGGAATGGAT+TGG 0.489614 1:-40561666 None:intergenic
GAATACCATCATCATTCAAA+AGG 0.492685 1:-40564691 None:intergenic
TTCAATCGTTGTTCGAAGCA+AGG 0.504367 1:+40562185 MsG0180002571.01.T01:CDS
TTCATATTCCTCAACCTCAC+CGG 0.509500 1:-40561956 None:intergenic
TCTGATGAAGTATTTGTCAA+TGG 0.510851 1:+40562941 MsG0180002571.01.T01:CDS
CAAGGGGTATGCTGAATTTG+GGG 0.511286 1:+40562913 MsG0180002571.01.T01:CDS
TGGGGTTGGTAGGCCTAGTG+AGG 0.512474 1:+40563025 MsG0180002571.01.T01:CDS
GCTGAAATAGAATGCTTAAG+TGG 0.513060 1:+40564165 MsG0180002571.01.T01:CDS
CGTTGATTAGATTTCAGTTG+AGG 0.525399 1:+40564910 MsG0180002571.01.T01:intron
ATATAAAAGGGACCCTCACT+AGG 0.526447 1:-40563038 None:intergenic
CTTCAATTTCAGGTACAAAA+GGG 0.526666 1:-40562017 None:intergenic
TGCAAACCTTAAGCCACTTG+TGG 0.528385 1:-40564266 None:intergenic
GGAATGTATGATGGAAAAGA+AGG 0.532095 1:-40561796 None:intergenic
AAGTTACCCTATTCATTGGA+AGG 0.536623 1:+40564721 MsG0180002571.01.T01:CDS
GTGGGTTGATCTCTTGAAGC+CGG 0.536695 1:-40564247 None:intergenic
AGGTTGAGGAATATGAAGGA+AGG 0.551335 1:+40561962 MsG0180002571.01.T01:CDS
ATGTATGATGGAAAAGAAGG+TGG 0.552215 1:-40561793 None:intergenic
GATGGAAAAGAAGGTGGGAA+TGG 0.556044 1:-40561787 None:intergenic
AGAGTGATAGTTGTTGGAGC+AGG 0.568437 1:+40561607 MsG0180002571.01.T01:CDS
TCACGAATTATGGGGTTGGT+AGG 0.576228 1:+40563015 MsG0180002571.01.T01:CDS
GAGAATGCAGAGTATGATGA+AGG 0.578252 1:+40561895 MsG0180002571.01.T01:CDS
GTATTTGTCAATGGAGATGG+TGG 0.579490 1:+40562950 MsG0180002571.01.T01:CDS
TGGGTCTGGGAATTCTGGCA+TGG 0.586713 1:+40562125 MsG0180002571.01.T01:CDS
AAGGCTAAGAATAAGGATAC+CGG 0.587025 1:+40561937 MsG0180002571.01.T01:CDS
CGGGGATGTCACGAATTATG+GGG 0.588744 1:+40563007 MsG0180002571.01.T01:CDS
GGGGTTGGTAGGCCTAGTGA+GGG 0.589928 1:+40563026 MsG0180002571.01.T01:CDS
GGAAAGTGATTCATTCAACT+GGG 0.594965 1:+40562058 MsG0180002571.01.T01:CDS
TGTATGATGGAAAAGAAGGT+GGG 0.596307 1:-40561792 None:intergenic
AGTTACCCTATTCATTGGAA+GGG 0.596925 1:+40564722 MsG0180002571.01.T01:CDS
GAAGTATTTGTCAATGGAGA+TGG 0.597497 1:+40562947 MsG0180002571.01.T01:CDS
GCTGCTACTGAGAGACCAGA+AGG 0.602816 1:-40561631 None:intergenic
GAAGGTGGGAATGGTTTGTG+AGG 0.606122 1:-40561778 None:intergenic
GGTGAGGTTGAGGAATATGA+AGG 0.608479 1:+40561958 MsG0180002571.01.T01:CDS
TGTGTTGGACTATCAAGGAG+AGG 0.616154 1:+40564761 MsG0180002571.01.T01:CDS
GGAGATGGTGGATTTAGTGA+TGG 0.618594 1:+40562962 MsG0180002571.01.T01:CDS
TTCTGAGAAATGGAAGGTGA+AGG 0.623363 1:+40561918 MsG0180002571.01.T01:CDS
GATGTCACGAATTATGGGGT+TGG 0.629113 1:+40563011 MsG0180002571.01.T01:CDS
GCATTGGATCTTATCAACCA+TGG 0.635858 1:+40562153 MsG0180002571.01.T01:CDS
AAGTATCCTATTATTGACGT+TGG 0.642218 1:+40563077 MsG0180002571.01.T01:CDS
TCAAGAGATCAACCCACAAG+TGG 0.648193 1:+40564253 MsG0180002571.01.T01:CDS
GCTTAGTAAAATGGTTTACG+GGG 0.650434 1:+40562989 MsG0180002571.01.T01:CDS
CTGCTACTGAGAGACCAGAA+GGG 0.656151 1:-40561630 None:intergenic
TGAAGGTTCTGAGAAATGGA+AGG 0.688803 1:+40561912 MsG0180002571.01.T01:CDS
TAAGAATAAGGATACCGGTG+AGG 0.724974 1:+40561942 MsG0180002571.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAACATGATA+AGG - Chr1:40564497-40564516 None:intergenic 10.0%
!! AAAATAAATAATATAAATGC+AGG + Chr1:40562878-40562897 MsG0180002571.01.T01:intron 10.0%
!! TAGAATAAATATATAATTGA+CGG + Chr1:40562721-40562740 MsG0180002571.01.T01:intron 10.0%
!!! TAAACTAATTAATTATCTTT+TGG + Chr1:40564654-40564673 MsG0180002571.01.T01:intron 10.0%
!!! TATATTTTTCATAACATTAA+AGG + Chr1:40563751-40563770 MsG0180002571.01.T01:intron 10.0%
!!! TTGATTTTAATGATAATTTA+TGG - Chr1:40563232-40563251 None:intergenic 10.0%
!! AAAACAAAACAAAAAACATT+CGG - Chr1:40562289-40562308 None:intergenic 15.0%
!! AAAATAAAGGATTAAATTGA+TGG + Chr1:40563305-40563324 MsG0180002571.01.T01:intron 15.0%
!! AACAAATTTATTTAAGTGAA+AGG + Chr1:40563886-40563905 MsG0180002571.01.T01:intron 15.0%
!! AGAATAAATATATAATTGAC+GGG + Chr1:40562722-40562741 MsG0180002571.01.T01:intron 15.0%
!! ATGTATTCAAGTATAATTTA+AGG - Chr1:40563264-40563283 None:intergenic 15.0%
!!! ATATCATTTTACTAAAAAGA+TGG + Chr1:40563933-40563952 MsG0180002571.01.T01:intron 15.0%
!!! CAAAAAAGAATAGTTTTTTT+AGG + Chr1:40564521-40564540 MsG0180002571.01.T01:intron 15.0%
!!! TAATTAATTATCTTTTGGTA+GGG + Chr1:40564659-40564678 MsG0180002571.01.T01:intron 15.0%
!!! TATTGTAAAAACTTTTCTTT+AGG - Chr1:40561820-40561839 None:intergenic 15.0%
!!! TATTTGACTTTAATAGTTTA+AGG + Chr1:40563352-40563371 MsG0180002571.01.T01:intron 15.0%
!!! TTTATTCTAAAAAAAGATTG+TGG - Chr1:40562710-40562729 None:intergenic 15.0%
!! AAAAATGGTTTATATTGTGT+TGG + Chr1:40564746-40564765 MsG0180002571.01.T01:CDS 20.0%
!! AAAGAAACATATCAAACTAT+AGG + Chr1:40562462-40562481 MsG0180002571.01.T01:intron 20.0%
!! AATATACGTCAAAGTATATA+TGG - Chr1:40564622-40564641 None:intergenic 20.0%
!! AATGTATGTTACATGAAAAT+GGG + Chr1:40564570-40564589 MsG0180002571.01.T01:intron 20.0%
!! ACCTAAAATAACATTTAGAT+GGG + Chr1:40563848-40563867 MsG0180002571.01.T01:intron 20.0%
!! AGAATGACATATATTTCAAT+AGG - Chr1:40563615-40563634 None:intergenic 20.0%
!! ATTGAAATATATGTCATTCT+AGG + Chr1:40563615-40563634 MsG0180002571.01.T01:intron 20.0%
!! TAATGTATGTTACATGAAAA+TGG + Chr1:40564569-40564588 MsG0180002571.01.T01:intron 20.0%
!! TACCTAAAATAACATTTAGA+TGG + Chr1:40563847-40563866 MsG0180002571.01.T01:intron 20.0%
!! TATACTTGAATACATCAATT+TGG + Chr1:40563269-40563288 MsG0180002571.01.T01:intron 20.0%
!! TCCATAAACAAGTAAAATAA+AGG + Chr1:40563292-40563311 MsG0180002571.01.T01:intron 20.0%
!! TGATTATACTCAAATATTTC+TGG + Chr1:40562563-40562582 MsG0180002571.01.T01:intron 20.0%
!!! AGATTTTATATAGAATTGGA+CGG + Chr1:40562633-40562652 MsG0180002571.01.T01:intron 20.0%
!!! CTAATTAATTATCTTTTGGT+AGG + Chr1:40564658-40564677 MsG0180002571.01.T01:intron 20.0%
!!! GGTAAGATTTTATATAGAAT+TGG + Chr1:40562629-40562648 MsG0180002571.01.T01:intron 20.0%
!!! TCCTTTATTTTACTTGTTTA+TGG - Chr1:40563296-40563315 None:intergenic 20.0%
!!! TTCAGAAGATTTTTTTTTGA+GGG - Chr1:40563502-40563521 None:intergenic 20.0%
!!! TTTCAGAAGATTTTTTTTTG+AGG - Chr1:40563503-40563522 None:intergenic 20.0%
! ACAAAAAAGAGAAGAAAGAA+TGG + Chr1:40561570-40561589 MsG0180002571.01.T01:exon 25.0%
! AGGTTATCTAACTATAAACA+AGG + Chr1:40563906-40563925 MsG0180002571.01.T01:intron 25.0%
! ATGCTTAGTAAAATGGTTTA+CGG + Chr1:40562987-40563006 MsG0180002571.01.T01:CDS 25.0%
! ATTGTGTTATGTGTTATGTT+AGG + Chr1:40564134-40564153 MsG0180002571.01.T01:intron 25.0%
! CCTAAAATAACATTTAGATG+GGG + Chr1:40563849-40563868 MsG0180002571.01.T01:intron 25.0%
! GATTACAAATACAGATTTGT+AGG - Chr1:40562385-40562404 None:intergenic 25.0%
! TACTTGACCTTCATATAAAA+GGG - Chr1:40563053-40563072 None:intergenic 25.0%
! TTTGATGTGTGTATCTTAAA+AGG - Chr1:40562312-40562331 None:intergenic 25.0%
!! AAATATATGTCATTCTAGGA+AGG + Chr1:40563619-40563638 MsG0180002571.01.T01:intron 25.0%
!! AACCATATTAGGCAAATTTT+AGG - Chr1:40563200-40563219 None:intergenic 25.0%
!! ACTTCATAGATTTTTCATGA+TGG - Chr1:40564837-40564856 None:intergenic 25.0%
!! ATATATGGTCACATTTCTTT+TGG - Chr1:40564607-40564626 None:intergenic 25.0%
!! GAAAAGTGCAAATATAATTG+TGG + Chr1:40564085-40564104 MsG0180002571.01.T01:intron 25.0%
!! TCAACAAAAAGGTTTTTACT+CGG - Chr1:40562495-40562514 None:intergenic 25.0%
!! TTTTATATGAAGGTCAAGTA+CGG + Chr1:40563053-40563072 MsG0180002571.01.T01:CDS 25.0%
!!! AAAATTGTTTTGCTTCACTA+TGG + Chr1:40561698-40561717 MsG0180002571.01.T01:CDS 25.0%
!!! AACATGTCGAATGATTTTTT+TGG + Chr1:40562438-40562457 MsG0180002571.01.T01:intron 25.0%
!!! ATCTTCTCTGTCTTTTTTTA+AGG + Chr1:40563544-40563563 MsG0180002571.01.T01:intron 25.0%
!!! TTTTCTTTAGGAATGTATGA+TGG - Chr1:40561808-40561827 None:intergenic 25.0%
AAGAATGTCCTCTTCAAAAA+TGG + Chr1:40564189-40564208 MsG0180002571.01.T01:CDS 30.0%
AAGTATCCTATTATTGACGT+TGG + Chr1:40563077-40563096 MsG0180002571.01.T01:CDS 30.0%
AATGAAAGAGTGATAGTTGT+TGG + Chr1:40561601-40561620 MsG0180002571.01.T01:CDS 30.0%
AATTAAGTCTCGAGAGTTAA+AGG + Chr1:40563112-40563131 MsG0180002571.01.T01:CDS 30.0%
ACGTTAGAGATAATTGACAT+TGG + Chr1:40563136-40563155 MsG0180002571.01.T01:intron 30.0%
AGAATTGGACGGATATATAT+GGG + Chr1:40562644-40562663 MsG0180002571.01.T01:intron 30.0%
ATTCAACTGGGTTTAAGAAT+GGG + Chr1:40562070-40562089 MsG0180002571.01.T01:CDS 30.0%
ATTCATTGGAAGGGAAAAAA+TGG + Chr1:40564731-40564750 MsG0180002571.01.T01:CDS 30.0%
CACCTAAAATTTGCCTAATA+TGG + Chr1:40563195-40563214 MsG0180002571.01.T01:intron 30.0%
GAATACCATCATCATTCAAA+AGG - Chr1:40564694-40564713 None:intergenic 30.0%
GAATAGATCAATATCAGCAA+CGG + Chr1:40564387-40564406 MsG0180002571.01.T01:intron 30.0%
GGATTATATAAGGAATGGAT+TGG - Chr1:40561669-40561688 None:intergenic 30.0%
GTACTTGACCTTCATATAAA+AGG - Chr1:40563054-40563073 None:intergenic 30.0%
TAATAGTTTAAGGACCAAAG+TGG + Chr1:40563362-40563381 MsG0180002571.01.T01:intron 30.0%
TAGAATTGGACGGATATATA+TGG + Chr1:40562643-40562662 MsG0180002571.01.T01:intron 30.0%
TATATTGTGTTGGACTATCA+AGG + Chr1:40564756-40564775 MsG0180002571.01.T01:CDS 30.0%
TCGTTTCATCATCAACAAAA+AGG - Chr1:40562506-40562525 None:intergenic 30.0%
TCTCTTTCGAGGATTATATA+AGG - Chr1:40561679-40561698 None:intergenic 30.0%
TGCTTAGTAAAATGGTTTAC+GGG + Chr1:40562988-40563007 MsG0180002571.01.T01:CDS 30.0%
TTCGAGGATTATATAAGGAA+TGG - Chr1:40561674-40561693 None:intergenic 30.0%
! ATAATTGACATTGGTTGCTT+TGG + Chr1:40563145-40563164 MsG0180002571.01.T01:intron 30.0%
! CAAAACAATTTTCTCTTTCG+AGG - Chr1:40561690-40561709 None:intergenic 30.0%
! CTTCAATTTCAGGTACAAAA+GGG - Chr1:40562020-40562039 None:intergenic 30.0%
! CTTTTGTACCTGAAATTGAA+GGG + Chr1:40562019-40562038 MsG0180002571.01.T01:CDS 30.0%
! GATTACCTTTTGAATGATGA+TGG + Chr1:40564686-40564705 MsG0180002571.01.T01:CDS 30.0%
! GGAAAGTTTGAAACCATATT+AGG - Chr1:40563211-40563230 None:intergenic 30.0%
! TCGAGACTTAATTTTGTGAA+AGG - Chr1:40563105-40563124 None:intergenic 30.0%
! TCTGATGAAGTATTTGTCAA+TGG + Chr1:40562941-40562960 MsG0180002571.01.T01:CDS 30.0%
! TTTTTCCCTTCCAATGAATA+GGG - Chr1:40564730-40564749 None:intergenic 30.0%
!! ATGAACATGTTCTTGTTGTT+GGG + Chr1:40562106-40562125 MsG0180002571.01.T01:CDS 30.0%
!!! CCCCATCTAAATGTTATTTT+AGG - Chr1:40563852-40563871 None:intergenic 30.0%
!!! TTTTTTCCCTTCCAATGAAT+AGG - Chr1:40564731-40564750 None:intergenic 30.0%
AAGGCTAAGAATAAGGATAC+CGG + Chr1:40561937-40561956 MsG0180002571.01.T01:CDS 35.0%
AAGGTTCCAACGTCAATAAT+AGG - Chr1:40563086-40563105 None:intergenic 35.0%
AAGTTACCCTATTCATTGGA+AGG + Chr1:40564721-40564740 MsG0180002571.01.T01:CDS 35.0%
AGTTACCCTATTCATTGGAA+GGG + Chr1:40564722-40564741 MsG0180002571.01.T01:CDS 35.0%
CATTCAACTGGGTTTAAGAA+TGG + Chr1:40562069-40562088 MsG0180002571.01.T01:CDS 35.0%
CTTAAGCATTCTATTTCAGC+TGG - Chr1:40564165-40564184 None:intergenic 35.0%
GCTGAAATAGAATGCTTAAG+TGG + Chr1:40564165-40564184 MsG0180002571.01.T01:CDS 35.0%
GCTTAGTAAAATGGTTTACG+GGG + Chr1:40562989-40563008 MsG0180002571.01.T01:CDS 35.0%
GGAAAGTGATTCATTCAACT+GGG + Chr1:40562058-40562077 MsG0180002571.01.T01:CDS 35.0%
TCAACTGTTCTGTGATAAAG+AGG - Chr1:40561877-40561896 None:intergenic 35.0%
TTCCAATGAATAGGGTAACT+TGG - Chr1:40564722-40564741 None:intergenic 35.0%
! AAGGGTTGAAGAGTTTTACT+GGG + Chr1:40562037-40562056 MsG0180002571.01.T01:CDS 35.0%
! ATGTATGATGGAAAAGAAGG+TGG - Chr1:40561796-40561815 None:intergenic 35.0%
! CCTTCAATTTCAGGTACAAA+AGG - Chr1:40562021-40562040 None:intergenic 35.0%
! CCTTTTGTACCTGAAATTGA+AGG + Chr1:40562018-40562037 MsG0180002571.01.T01:CDS 35.0%
! CGTTGATTAGATTTCAGTTG+AGG + Chr1:40564910-40564929 MsG0180002571.01.T01:intron 35.0%
! GAAGTATTTGTCAATGGAGA+TGG + Chr1:40562947-40562966 MsG0180002571.01.T01:CDS 35.0%
! GACTCTATTATTTTCTGCAC+CGG + Chr1:40564228-40564247 MsG0180002571.01.T01:CDS 35.0%
! GATGAACATGTTCTTGTTGT+TGG + Chr1:40562105-40562124 MsG0180002571.01.T01:CDS 35.0%
! GATGGTGATGCTTAGTAAAA+TGG + Chr1:40562980-40562999 MsG0180002571.01.T01:CDS 35.0%
! GGAATATGAAGGAAGGTTTT+TGG + Chr1:40561969-40561988 MsG0180002571.01.T01:CDS 35.0%
! GGAATGTATGATGGAAAAGA+AGG - Chr1:40561799-40561818 None:intergenic 35.0%
! GTTAGCTGTTGATTATACGT+AGG + Chr1:40562796-40562815 MsG0180002571.01.T01:intron 35.0%
! TAAATTTGAGACAGAGCACA+TGG - Chr1:40562360-40562379 None:intergenic 35.0%
! TGTATGATGGAAAAGAAGGT+GGG - Chr1:40561795-40561814 None:intergenic 35.0%
!! ATGATGAAGGTTCTGAGAAA+TGG + Chr1:40561908-40561927 MsG0180002571.01.T01:CDS 35.0%
!! GCAATTGACCATTTTTGAAG+AGG - Chr1:40564200-40564219 None:intergenic 35.0%
!!! GCTCGCAAAATTGTTTTTTG+AGG - Chr1:40561756-40561775 None:intergenic 35.0%
AGCCAAGTTACCCTATTCAT+TGG + Chr1:40564717-40564736 MsG0180002571.01.T01:CDS 40.0%
AGGTTGAGGAATATGAAGGA+AGG + Chr1:40561962-40561981 MsG0180002571.01.T01:CDS 40.0%
ATATAAAAGGGACCCTCACT+AGG - Chr1:40563041-40563060 None:intergenic 40.0%
ATGAATAGGGTAACTTGGCT+TGG - Chr1:40564717-40564736 None:intergenic 40.0%
CTATCAAGGAGAGGTTTCTA+TGG + Chr1:40564770-40564789 MsG0180002571.01.T01:CDS 40.0%
CTCTTCAACCCTTCAATTTC+AGG - Chr1:40562030-40562049 None:intergenic 40.0%
GAAGGTGAAGGCTAAGAATA+AGG + Chr1:40561930-40561949 MsG0180002571.01.T01:CDS 40.0%
GAGAATGCAGAGTATGATGA+AGG + Chr1:40561895-40561914 MsG0180002571.01.T01:CDS 40.0%
GCATTGGATCTTATCAACCA+TGG + Chr1:40562153-40562172 MsG0180002571.01.T01:CDS 40.0%
GGGAAAGTGATTCATTCAAC+TGG + Chr1:40562057-40562076 MsG0180002571.01.T01:CDS 40.0%
GTATTTGTCAATGGAGATGG+TGG + Chr1:40562950-40562969 MsG0180002571.01.T01:CDS 40.0%
GTTGTTGTGACATGACGAAT+GGG + Chr1:40564110-40564129 MsG0180002571.01.T01:intron 40.0%
TAAGAATAAGGATACCGGTG+AGG + Chr1:40561942-40561961 MsG0180002571.01.T01:CDS 40.0%
TAATTGACGGGATGAATGAG+TGG + Chr1:40562734-40562753 MsG0180002571.01.T01:intron 40.0%
TGAATAGGGTAACTTGGCTT+GGG - Chr1:40564716-40564735 None:intergenic 40.0%
TGTTGTTGTGACATGACGAA+TGG + Chr1:40564109-40564128 MsG0180002571.01.T01:intron 40.0%
TTCAATCGTTGTTCGAAGCA+AGG + Chr1:40562185-40562204 MsG0180002571.01.T01:CDS 40.0%
TTCATATTCCTCAACCTCAC+CGG - Chr1:40561959-40561978 None:intergenic 40.0%
TTCTGGCATGGAAATTGCAT+TGG + Chr1:40562137-40562156 MsG0180002571.01.T01:CDS 40.0%
! CAGGTTCACATTTTGTCAAG+GGG + Chr1:40562897-40562916 MsG0180002571.01.T01:intron 40.0%
! GAAGGGTTGAAGAGTTTTAC+TGG + Chr1:40562036-40562055 MsG0180002571.01.T01:CDS 40.0%
! GCAGGTTCACATTTTGTCAA+GGG + Chr1:40562896-40562915 MsG0180002571.01.T01:intron 40.0%
! TCAAGCGAGTTACTTTGCTT+GGG - Chr1:40563983-40564002 None:intergenic 40.0%
! TCAAGGGGTATGCTGAATTT+GGG + Chr1:40562912-40562931 MsG0180002571.01.T01:CDS 40.0%
! TGAGGGTCCCTTTTATATGA+AGG + Chr1:40563043-40563062 MsG0180002571.01.T01:CDS 40.0%
! TGCAGGTTCACATTTTGTCA+AGG + Chr1:40562895-40562914 MsG0180002571.01.T01:intron 40.0%
! TTCAAGCGAGTTACTTTGCT+TGG - Chr1:40563984-40564003 None:intergenic 40.0%
! TTCTGAGAAATGGAAGGTGA+AGG + Chr1:40561918-40561937 MsG0180002571.01.T01:CDS 40.0%
!! ATGTTCTTGTTGTTGGGTCT+GGG + Chr1:40562112-40562131 MsG0180002571.01.T01:CDS 40.0%
!! CGATTGAAGTTTTAGCACCA+TGG - Chr1:40562173-40562192 None:intergenic 40.0%
!! TGAAGGTTCTGAGAAATGGA+AGG + Chr1:40561912-40561931 MsG0180002571.01.T01:CDS 40.0%
!!! TGAAGGAAGGTTTTTGGTTG+TGG + Chr1:40561975-40561994 MsG0180002571.01.T01:CDS 40.0%
AATCTTACCACCTTCCGTGA+AGG - Chr1:40562618-40562637 None:intergenic 45.0%
ACGGGGATGTCACGAATTAT+GGG + Chr1:40563006-40563025 MsG0180002571.01.T01:CDS 45.0%
AGAGTGATAGTTGTTGGAGC+AGG + Chr1:40561607-40561626 MsG0180002571.01.T01:CDS 45.0%
ATCAACCCACAAGTGGCTTA+AGG + Chr1:40564260-40564279 MsG0180002571.01.T01:CDS 45.0%
ATCTTACCACCTTCCGTGAA+GGG - Chr1:40562617-40562636 None:intergenic 45.0%
CTTCCGTGAAGGGAATATGT+GGG - Chr1:40562607-40562626 None:intergenic 45.0%
GATGGAAAAGAAGGTGGGAA+TGG - Chr1:40561790-40561809 None:intergenic 45.0%
GATGTCACGAATTATGGGGT+TGG + Chr1:40563011-40563030 MsG0180002571.01.T01:CDS 45.0%
GCAAACCTTAAGCCACTTGT+GGG - Chr1:40564268-40564287 None:intergenic 45.0%
GGTGAGGTTGAGGAATATGA+AGG + Chr1:40561958-40561977 MsG0180002571.01.T01:CDS 45.0%
TAAGGACCAAAGTGGTGAGA+GGG + Chr1:40563370-40563389 MsG0180002571.01.T01:intron 45.0%
TACGGGGATGTCACGAATTA+TGG + Chr1:40563005-40563024 MsG0180002571.01.T01:CDS 45.0%
TCAAGAGATCAACCCACAAG+TGG + Chr1:40564253-40564272 MsG0180002571.01.T01:CDS 45.0%
TGCAAACCTTAAGCCACTTG+TGG - Chr1:40564269-40564288 None:intergenic 45.0%
TGTGTTGGACTATCAAGGAG+AGG + Chr1:40564761-40564780 MsG0180002571.01.T01:CDS 45.0%
TTAAGGACCAAAGTGGTGAG+AGG + Chr1:40563369-40563388 MsG0180002571.01.T01:intron 45.0%
TTCCCCACATATTCCCTTCA+CGG + Chr1:40562601-40562620 MsG0180002571.01.T01:intron 45.0%
TTCCGTGAAGGGAATATGTG+GGG - Chr1:40562606-40562625 None:intergenic 45.0%
! CAAGCGAGTTACTTTGCTTG+GGG - Chr1:40563982-40564001 None:intergenic 45.0%
! CAAGGGGTATGCTGAATTTG+GGG + Chr1:40562913-40562932 MsG0180002571.01.T01:CDS 45.0%
! GTCAAGGGGTATGCTGAATT+TGG + Chr1:40562911-40562930 MsG0180002571.01.T01:CDS 45.0%
! TCACGAATTATGGGGTTGGT+AGG + Chr1:40563015-40563034 MsG0180002571.01.T01:CDS 45.0%
!! CATGTTCTTGTTGTTGGGTC+TGG + Chr1:40562111-40562130 MsG0180002571.01.T01:CDS 45.0%
!! GGAGATGGTGGATTTAGTGA+TGG + Chr1:40562962-40562981 MsG0180002571.01.T01:CDS 45.0%
!! GGTGAGAGGGTATTGGTTTA+AGG + Chr1:40563383-40563402 MsG0180002571.01.T01:intron 45.0%
!!! AGGTTTTTGGTTGTGGCTAG+TGG + Chr1:40561982-40562001 MsG0180002571.01.T01:CDS 45.0%
CATATTCCCTTCACGGAAGG+TGG + Chr1:40562608-40562627 MsG0180002571.01.T01:intron 50.0%
CCAATACCCTCTCACCACTT+TGG - Chr1:40563379-40563398 None:intergenic 50.0%
CCACATATTCCCTTCACGGA+AGG + Chr1:40562605-40562624 MsG0180002571.01.T01:intron 50.0%
CCTTCCGTGAAGGGAATATG+TGG - Chr1:40562608-40562627 None:intergenic 50.0%
CGGGGATGTCACGAATTATG+GGG + Chr1:40563007-40563026 MsG0180002571.01.T01:CDS 50.0%
CTGCTACTGAGAGACCAGAA+GGG - Chr1:40561633-40561652 None:intergenic 50.0%
GTTGTTGGGTCTGGGAATTC+TGG + Chr1:40562120-40562139 MsG0180002571.01.T01:CDS 50.0%
TAAGGATACCGGTGAGGTTG+AGG + Chr1:40561948-40561967 MsG0180002571.01.T01:CDS 50.0%
! CCAAAGTGGTGAGAGGGTAT+TGG + Chr1:40563376-40563395 MsG0180002571.01.T01:intron 50.0%
! GAAGGTGGGAATGGTTTGTG+AGG - Chr1:40561781-40561800 None:intergenic 50.0%
!! GTGGGTTGATCTCTTGAAGC+CGG - Chr1:40564250-40564269 None:intergenic 50.0%
GCTGCTACTGAGAGACCAGA+AGG - Chr1:40561634-40561653 None:intergenic 55.0%
TGGGTCTGGGAATTCTGGCA+TGG + Chr1:40562125-40562144 MsG0180002571.01.T01:CDS 55.0%
GTTGTTGGAGCAGGCCCTTC+TGG + Chr1:40561616-40561635 MsG0180002571.01.T01:CDS 60.0%
!! GGGGTTGGTAGGCCTAGTGA+GGG + Chr1:40563026-40563045 MsG0180002571.01.T01:CDS 60.0%
!! TGGGGTTGGTAGGCCTAGTG+AGG + Chr1:40563025-40563044 MsG0180002571.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 40561568 40564952 40561568 ID=MsG0180002571.01;Name=MsG0180002571.01
Chr1 mRNA 40561568 40564952 40561568 ID=MsG0180002571.01.T01;Parent=MsG0180002571.01;Name=MsG0180002571.01.T01;_AED=0.50;_eAED=0.50;_QI=21|0.75|0.6|1|1|1|5|0|385
Chr1 exon 40561568 40562206 40561568 ID=MsG0180002571.01.T01:exon:37148;Parent=MsG0180002571.01.T01
Chr1 exon 40562900 40563133 40562900 ID=MsG0180002571.01.T01:exon:37149;Parent=MsG0180002571.01.T01
Chr1 exon 40564156 40564281 40564156 ID=MsG0180002571.01.T01:exon:37150;Parent=MsG0180002571.01.T01
Chr1 exon 40564680 40564826 40564680 ID=MsG0180002571.01.T01:exon:37151;Parent=MsG0180002571.01.T01
Chr1 exon 40564920 40564952 40564920 ID=MsG0180002571.01.T01:exon:37152;Parent=MsG0180002571.01.T01
Chr1 five_prime_UTR 40561568 40561588 40561568 ID=MsG0180002571.01.T01:five_prime_utr;Parent=MsG0180002571.01.T01
Chr1 CDS 40561589 40562206 40561589 ID=MsG0180002571.01.T01:cds;Parent=MsG0180002571.01.T01
Chr1 CDS 40562900 40563133 40562900 ID=MsG0180002571.01.T01:cds;Parent=MsG0180002571.01.T01
Chr1 CDS 40564156 40564281 40564156 ID=MsG0180002571.01.T01:cds;Parent=MsG0180002571.01.T01
Chr1 CDS 40564680 40564826 40564680 ID=MsG0180002571.01.T01:cds;Parent=MsG0180002571.01.T01
Chr1 CDS 40564920 40564952 40564920 ID=MsG0180002571.01.T01:cds;Parent=MsG0180002571.01.T01
Gene Sequence

>MsG0180002571.01.T01

ATGGAGAAAGAGAATGAAAGAGTGATAGTTGTTGGAGCAGGCCCTTCTGGTCTCTCAGTAGCAGCAAGCTTAACAAACCAATCCATTCCTTATATAATCCTCGAAAGAGAAAATTGTTTTGCTTCACTATGGAAAAAATATTCATACGATCGTCTTCATCTTCACCTCAAAAAACAATTTTGCGAGCTTCCTCACAAACCATTCCCACCTTCTTTTCCATCATACATTCCTAAAGAAAAGTTTTTACAATACTTAGAAGACTATGTTTCACACTTCAAAATCAATCCTCTTTATCACAGAACAGTTGAGAATGCAGAGTATGATGAAGGTTCTGAGAAATGGAAGGTGAAGGCTAAGAATAAGGATACCGGTGAGGTTGAGGAATATGAAGGAAGGTTTTTGGTTGTGGCTAGTGGTGAAACTAGTGACCCTTTTGTACCTGAAATTGAAGGGTTGAAGAGTTTTACTGGGAAAGTGATTCATTCAACTGGGTTTAAGAATGGGAGAGAGTTTAAAGATGAACATGTTCTTGTTGTTGGGTCTGGGAATTCTGGCATGGAAATTGCATTGGATCTTATCAACCATGGTGCTAAAACTTCAATCGTTGTTCGAAGCAAGGTTCACATTTTGTCAAGGGGTATGCTGAATTTGGGGTTGTTTCTGATGAAGTATTTGTCAATGGAGATGGTGGATTTAGTGATGGTGATGCTTAGTAAAATGGTTTACGGGGATGTCACGAATTATGGGGTTGGTAGGCCTAGTGAGGGTCCCTTTTATATGAAGGTCAAGTACGGCAAGTATCCTATTATTGACGTTGGAACCTTTCACAAAATTAAGTCTCGAGAGTTAAAGGTTTTGCCAGCTGAAATAGAATGCTTAAGTGGCAAGAATGTCCTCTTCAAAAATGGTCAATTGCACTCTTTTGACTCTATTATTTTCTGCACCGGCTTCAAGAGATCAACCCACAAGTGGCTTAAGGGTGATGATTACCTTTTGAATGATGATGGTATTCCCAAGCCAAGTTACCCTATTCATTGGAAGGGAAAAAATGGTTTATATTGTGTTGGACTATCAAGGAGAGGTTTCTATGGAGCTGCTGCAGATGCAAAAAATATAGCAAATGATATTTCAGTTGAGGTTAGAGATACTCGAGTATAA
Protein sequence

>MsG0180002571.01.T01

MEKENERVIVVGAGPSGLSVAASLTNQSIPYIILERENCFASLWKKYSYDRLHLHLKKQFCELPHKPFPPSFPSYIPKEKFLQYLEDYVSHFKINPLYHRTVENAEYDEGSEKWKVKAKNKDTGEVEEYEGRFLVVASGETSDPFVPEIEGLKSFTGKVIHSTGFKNGREFKDEHVLVVGSGNSGMEIALDLINHGAKTSIVVRSKVHILSRGMLNLGLFLMKYLSMEMVDLVMVMLSKMVYGDVTNYGVGRPSEGPFYMKVKYGKYPIIDVGTFHKIKSRELKVLPAEIECLSGKNVLFKNGQLHSFDSIIFCTGFKRSTHKWLKGDDYLLNDDGIPKPSYPIHWKGKNGLYCVGLSRRGFYGAAADAKNIANDISVEVRDTRV*