AlfalfaGEDB | MsG0180002949.01

M. sativa cultivar ZhongmuNo.1 / MsG0180002949.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002949.01.T01	XP_003588957.1	70.934	289	64	6	70	351	121	396	2.70E-127	380

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002949.01.T01	G7I9U7	70.934	289	64	6	70	351	121	396	1.29e-127	380

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
MsG0180000330.01	MsG0180002949.01	PPI
MsG0180001056.01	MsG0180002949.01	PPI
MsG0180002191.01	MsG0180002949.01	PPI
MsG0180002493.01	MsG0180002949.01	PPI
MsG0180002949.01	MsG0680035093.01	PPI
MsG0180002949.01	MsG0780037756.01	PPI
MsG0180002949.01	MsG0680035305.01	PPI
MsG0180002949.01	MsG0880043152.01	PPI
MsG0180002949.01	MsG0680035283.01	PPI
MsG0180002949.01	MsG0780038440.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002949.01.T01	MTR_1g015730	70.934	289	64	6	70	351	121	396	3.27e-131	380
MsG0180002949.01.T01	MTR_1g029650	62.838	296	92	8	70	350	112	404	2.14e-115	340
MsG0180002949.01.T01	MTR_5g058620	61.594	276	78	9	101	351	11	283	1.17e-101	301
MsG0180002949.01.T01	MTR_1g058870	61.594	276	78	9	101	351	11	283	1.17e-101	301
MsG0180002949.01.T01	MTR_7g078900	51.935	310	123	9	66	351	117	424	1.62e-90	278
MsG0180002949.01.T01	MTR_1g015740	62.083	240	55	5	119	351	118	328	3.63e-89	271
MsG0180002949.01.T01	MTR_4g013185	49.684	316	125	9	66	350	114	426	4.87e-88	271
MsG0180002949.01.T01	MTR_4g013175	48.580	317	129	9	66	351	114	427	1.28e-84	263
MsG0180002949.01.T01	MTR_4g013180	50.347	288	116	7	72	334	102	387	1.35e-83	261
MsG0180002949.01.T01	MTR_1g088120	47.040	321	132	9	62	350	97	411	1.87e-80	251

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 71 sgRNAs with CRISPR-Local

Find 347 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGGTCATCAATATTAAA+AGG	0.197166	1:-53669731	None:intergenic
AACCGAAACTTATAAAGTGG+TGG	0.627190	1:+53673683	MsG0180002949.01.T01:CDS
AACTAGTGCTAATGCATATT+GGG	0.260109	1:+53674286	MsG0180002949.01.T01:CDS
AATCAATACACTGTTCCTTG+TGG	0.661217	1:+53673915	MsG0180002949.01.T01:CDS
AATCCTTTCTTCAACTTATA+TGG	0.195948	1:+53669795	MsG0180002949.01.T01:CDS
AATGGTCATCAATATTAAAA+GGG	0.507085	1:-53669730	None:intergenic
AATTTGGGGTTGATGATTCT+TGG	0.275914	1:+53674045	MsG0180002949.01.T01:CDS
ACACCAATAGTTGGCGAGTT+AGG	0.383207	1:-53673954	None:intergenic
ACTAGTGCTAATGCATATTG+GGG	0.613855	1:+53674287	MsG0180002949.01.T01:CDS
ACTTGGGTGGTGATGATGTT+TGG	0.382252	1:+53673748	MsG0180002949.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATATTCTCCACATTGG+GGG	-	Chr1:53671790-53671809	None:intergenic	30.0%
AAAAGTTGGATCGGAAATTA+GGG	-	Chr1:53673272-53673291	None:intergenic	30.0%
AAAGATGATTCACTTATGCA+TGG	-	Chr1:53670560-53670579	None:intergenic	30.0%
AAATGAGTAAACCACCAAAT+TGG	-	Chr1:53671664-53671683	None:intergenic	30.0%
AACTAGTGCTAATGCATATT+GGG	+	Chr1:53674286-53674305	MsG0180002949.01.T01:CDS	30.0%
AAGACTGCATCCAAAAAATT+TGG	+	Chr1:53673606-53673625	MsG0180002949.01.T01:CDS	30.0%
AATAGTTAAAACTCTCCCAA+TGG	-	Chr1:53671744-53671763	None:intergenic	30.0%
AATCATCTTTCATTGGTATG+TGG	+	Chr1:53670570-53670589	MsG0180002949.01.T01:intron	30.0%
AATGTTATTCTTTGTTCGAG+TGG	-	Chr1:53670524-53670543	None:intergenic	30.0%
AATTGAAGAAGAGATCTAAC+GGG	-	Chr1:53673223-53673242	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	53669647	53674352	53669647	ID=MsG0180002949.01;Name=MsG0180002949.01
Chr1	mRNA	53669647	53674352	53669647	ID=MsG0180002949.01.T01;Parent=MsG0180002949.01;Name=MsG0180002949.01.T01;_AED=0.37;_eAED=0.38;_QI=0\|0\|0\|1\|1\|1\|2\|0\|351
Chr1	exon	53669647	53669848	53669647	ID=MsG0180002949.01.T01:exon:2745;Parent=MsG0180002949.01.T01
Chr1	exon	53673499	53674352	53673499	ID=MsG0180002949.01.T01:exon:2746;Parent=MsG0180002949.01.T01
Chr1	CDS	53669647	53669848	53669647	ID=MsG0180002949.01.T01:cds;Parent=MsG0180002949.01.T01
Chr1	CDS	53673499	53674352	53673499	ID=MsG0180002949.01.T01:cds;Parent=MsG0180002949.01.T01

Gene Sequence

>MsG0180002949.01.T01

ATGATTCGAACACTTATTCATTGTGCCACTTTCAGTACATTGTGGCATCATATCAGGTCTTGGATCGGAATGTCAGGTGTTGACCCTTTTAATATTGATGACCATTTATTTCAGTTTATTCACTCTACAGGTCACTCGAAGGCTCGTCAATCCTTTCTTCAACTTATATGGTTACTTTGTGTCTGGTTGGTTTGGAACGAACGAAGTACTGGAATAGTTGGTTCCTGTAATGGATTGCTTCGTTTGGATGTCGATTCTGAAAGTTGTCAAGATTTAGAGAACTGGCTCCGTGTTTGGAACCCAGCCACCAAGACTGCATCCAAAAAATTTGGGTATACTCGCGATCCCAATAAAAATTTTCACTTTGCCTTTGGATGTGATAATTCAACCGAAACTTATAAAGTGGTGGCGTATTGTAATCGAGAGACAACATCGGAGGTCAGAGTTCTCAACTTGGGTGGTGATGATGTTTGGAGGAATATTGAATGTTTTCCTGTTGTTCTTGGCCACAAACATGTGTATTTGAGTGGCACTATGAACTGGTTGGCTACTATTCACAACAGTGATACTGTGGAACACTCTGCTATTGTTTCACTTGATCTGGAGACGGAGGCGTACAATCAATACACTGTTCCTTGTGGTGTTGATGAAGTGCTGCCTAACTCGCCAACTATTGGTGTGCTTGGAGGCTGCCTTTGTTTTTCTTATTTGCACAGGGGAGATGATTTTGTTATATGGAAAATGAAGGAATTTGGGGTTGATGATTCTTGGACTCAATTACTTAAAGTTAGTTACCATGATCTTCTAATAGATTATGATGACTTTAGTGACACTACCTATTTTCAGTTGGTGCCATTATTTCTTTCGGAGGATGGTGATTCACTGGTACTTCATAGCAATCTAGAATCCCAAACAATTCTCTATAACAGGAGAGATAATAGAGCAAATCGAACAGAAATTATTGCAAGTAGAACTACTGCTGATAATAGAACTAGTGCTAATGCATATTGGGGCTATGAGTTGGATTATGTTGAAAGCTTAGTTCCAATTTTTTGA

Protein sequence

>MsG0180002949.01.T01

MIRTLIHCATFSTLWHHIRSWIGMSGVDPFNIDDHLFQFIHSTGHSKARQSFLQLIWLLCVWLVWNERSTGIVGSCNGLLRLDVDSESCQDLENWLRVWNPATKTASKKFGYTRDPNKNFHFAFGCDNSTETYKVVAYCNRETTSEVRVLNLGGDDVWRNIECFPVVLGHKHVYLSGTMNWLATIHNSDTVEHSAIVSLDLETEAYNQYTVPCGVDEVLPNSPTIGVLGGCLCFSYLHRGDDFVIWKMKEFGVDDSWTQLLKVSYHDLLIDYDDFSDTTYFQLVPLFLSEDGDSLVLHSNLESQTILYNRRDNRANRTEIIASRTTADNRTSANAYWGYELDYVESLVPIF*