AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003294.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003294.01.T01 MTR_1g057020 97.653 213 5 0 1 213 1 213 5.67e-153 422
MsG0180003294.01.T01 MTR_2g094790 75.385 195 48 0 6 200 10 204 2.16e-113 322
MsG0180003294.01.T01 MTR_7g101290 72.727 209 55 1 6 212 11 219 6.74e-113 322
MsG0180003294.01.T01 MTR_0041s0100 72.727 209 55 1 6 212 11 219 6.74e-113 322
MsG0180003294.01.T01 MTR_1g064260 71.770 209 57 1 6 212 10 218 6.94e-112 319
MsG0180003294.01.T01 MTR_3g113710 72.249 209 58 0 5 213 11 219 4.68e-111 317
MsG0180003294.01.T01 MTR_4g058740 75.648 193 47 0 8 200 12 204 3.59e-110 314
MsG0180003294.01.T01 MTR_3g113710 78.571 182 39 0 5 186 11 192 1.75e-106 306
MsG0180003294.01.T01 MTR_1g015830 62.911 213 78 1 1 212 1 213 6.36e-99 286
MsG0180003294.01.T01 MTR_1g064260 72.222 162 43 1 53 212 1 162 1.16e-83 246
MsG0180003294.01.T01 MTR_3g113710 72.222 144 40 0 70 213 10 153 6.37e-71 213
MsG0180003294.01.T01 MTR_1g015830 62.112 161 60 1 53 212 1 161 5.49e-70 211
MsG0180003294.01.T01 MTR_1g024145 71.845 103 29 0 24 126 150 252 4.38e-48 158
MsG0180003294.01.T01 MTR_1g024850 67.143 70 23 0 6 75 14 83 1.09e-31 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003294.01.T01 AT5G06300 83.645 214 33 1 1 212 1 214 5.36e-131 367
MsG0180003294.01.T01 AT2G35990 75.980 204 49 0 1 204 1 204 2.12e-118 335
MsG0180003294.01.T01 AT2G37210 75.122 205 50 1 5 208 10 214 2.15e-115 328
MsG0180003294.01.T01 AT3G53450 75.122 205 50 1 5 208 10 214 1.84e-114 325
MsG0180003294.01.T01 AT2G28305 71.090 211 60 1 3 212 2 212 1.01e-112 321
MsG0180003294.01.T01 AT2G37210 66.812 229 51 2 5 208 10 238 2.79e-109 313
MsG0180003294.01.T01 AT2G28305 77.473 182 41 0 3 184 2 183 7.83e-107 305
MsG0180003294.01.T01 AT5G03270 72.487 189 52 0 2 190 12 200 5.25e-103 296
MsG0180003294.01.T01 AT4G35190 69.000 200 62 0 1 200 1 200 1.01e-101 294
MsG0180003294.01.T01 AT5G03270 73.224 183 49 0 2 184 12 194 3.61e-100 288
MsG0180003294.01.T01 AT4G35190 72.283 184 51 0 1 184 1 184 4.38e-98 283
MsG0180003294.01.T01 AT5G11950 59.524 210 84 1 2 210 3 212 2.73e-92 270
MsG0180003294.01.T01 AT5G11950 59.524 210 84 1 2 210 3 212 2.73e-92 270
MsG0180003294.01.T01 AT5G11950 59.524 210 84 1 2 210 3 212 2.73e-92 270
MsG0180003294.01.T01 AT2G35990 75.658 152 37 0 53 204 1 152 1.09e-84 248
MsG0180003294.01.T01 AT2G35990 75.658 152 37 0 53 204 1 152 1.09e-84 248
MsG0180003294.01.T01 AT2G28305 76.087 138 33 0 3 140 2 139 1.73e-78 232
MsG0180003294.01.T01 AT5G11950 60.377 159 62 1 53 210 1 159 3.12e-66 201
MsG0180003294.01.T01 AT5G26140 49.650 143 50 1 80 200 6 148 9.97e-40 134
MsG0180003294.01.T01 AT5G26140 48.062 129 45 1 94 200 1 129 1.35e-32 115
MsG0180003294.01.T01 AT5G26140 51.020 98 48 0 103 200 17 114 4.28e-28 103

Find 76 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGGATTGATTTGGTTTA+TGG 0.101186 1:+60145423 MsG0180003294.01.T01:CDS
TTTGAAACATTAGGTGGATA+TGG 0.215130 1:+60147016 MsG0180003294.01.T01:intron
GTCTACTGTGGTAGCAGTTC+AGG 0.251931 1:+60144480 MsG0180003294.01.T01:CDS
CTAGTTCAACAGCAGCTTCT+TGG 0.259502 1:-60144518 None:intergenic
GATGGCGGACGCCATGTTCT+TGG 0.292130 1:+60145492 MsG0180003294.01.T01:CDS
AATCCTCTTAAACTTTGATT+TGG 0.295009 1:-60144454 None:intergenic
GATGCCTTCATTGCCCTTCC+TGG 0.296660 1:+60146640 MsG0180003294.01.T01:CDS
AGATAACTGGTGACCCTATT+GGG 0.297013 1:+60146560 MsG0180003294.01.T01:intron
ACGATAATGCGACGTGCCTT+TGG 0.334696 1:-60148485 None:intergenic
GGAATTGGTTAGGGGATTAG+AGG 0.346976 1:+60148526 MsG0180003294.01.T01:CDS
CAGATAACTGGTGACCCTAT+TGG 0.351597 1:+60146559 MsG0180003294.01.T01:intron
TCATATGGCAACTTCTGATT+CGG 0.369297 1:-60148815 None:intergenic
AAACCAAATCAAAGTTTAAG+AGG 0.371717 1:+60144451 MsG0180003294.01.T01:CDS
CGATAATGCGACGTGCCTTT+GGG 0.375378 1:-60148484 None:intergenic
TGGAACCCTTGAAGAATTGC+TGG 0.377448 1:+60147036 MsG0180003294.01.T01:CDS
ACTCAATGGTTTACCTCTCT+TGG 0.379537 1:-60146428 None:intergenic
GGTTGAGAGAAGGATTGATT+TGG 0.380510 1:+60145416 MsG0180003294.01.T01:intron
ACAGCTCTCACTTCCCCAAT+AGG 0.386608 1:-60146574 None:intergenic
AATTGCTGGAAATTATTACT+TGG 0.394745 1:+60147050 MsG0180003294.01.T01:CDS
AGGTGGTAGTGTGGGTCTGA+TGG 0.405577 1:+60145446 MsG0180003294.01.T01:CDS
ATGGCGGACGCCATGTTCTT+GGG 0.414120 1:+60145493 MsG0180003294.01.T01:CDS
GGTGGTAGTGTGGGTCTGAT+GGG 0.415803 1:+60145447 MsG0180003294.01.T01:CDS
GTTTATGGAGGTGGTAGTGT+GGG 0.418025 1:+60145438 MsG0180003294.01.T01:CDS
CCACAGCCAAGGAATTGGTT+AGG 0.425578 1:+60148516 MsG0180003294.01.T01:CDS
GCATCAAAGGAAAGCTGAAA+TGG 0.425795 1:+60146606 MsG0180003294.01.T01:CDS
ACAGCAGCTTCTTGGTAAGT+AGG 0.426650 1:-60144510 None:intergenic
ATTGATTTGGTTTATGGAGG+TGG 0.437781 1:+60145429 MsG0180003294.01.T01:CDS
AGCATCCACAGCCAAGGAAT+TGG 0.439181 1:+60148511 MsG0180003294.01.T01:CDS
CATATGGCAACTTCTGATTC+GGG 0.447870 1:-60148814 None:intergenic
CACAGCCAAGGAATTGGTTA+GGG 0.451858 1:+60148517 MsG0180003294.01.T01:CDS
TCTCTTGGCATGAGACTCCT+AGG 0.453788 1:-60146413 None:intergenic
ATTATTACTTGGGCTCAACT+TGG 0.456278 1:+60147061 MsG0180003294.01.T01:CDS
GAAAGCTGAAATGGCTAGGC+AGG 0.460252 1:+60146615 MsG0180003294.01.T01:CDS
GTTTATGCAGTGTTATCCCT+AGG 0.460934 1:+60146396 MsG0180003294.01.T01:intron
GGTTTATGGAGGTGGTAGTG+TGG 0.474379 1:+60145437 MsG0180003294.01.T01:CDS
GATACATATTCATCTTGCTC+AGG 0.480241 1:-60148757 None:intergenic
ATTGACAAGGCCGTTGATGA+AGG 0.480397 1:+60148452 MsG0180003294.01.T01:CDS
CAGCTCTCACTTCCCCAATA+GGG 0.481416 1:-60146573 None:intergenic
ATTGCTGGAAATTATTACTT+GGG 0.482520 1:+60147051 MsG0180003294.01.T01:CDS
TAATTTCCAGCAATTCTTCA+AGG 0.488686 1:-60147042 None:intergenic
TCTTTCTTCCCACACCAACT+TGG 0.497589 1:-60148779 None:intergenic
AGGATTGATTTGGTTTATGG+AGG 0.498565 1:+60145426 MsG0180003294.01.T01:CDS
TGGTCGAAAATGCAGATAAC+TGG 0.500494 1:+60146547 MsG0180003294.01.T01:intron
CATACCAGGAAGGGCAATGA+AGG 0.504465 1:-60146644 None:intergenic
TTATTACTTGGGCTCAACTT+GGG 0.511742 1:+60147062 MsG0180003294.01.T01:CDS
CAATGTTGAAGGTTGAGAGA+AGG 0.514544 1:+60145406 MsG0180003294.01.T01:intron
AATTTCCAGCAATTCTTCAA+GGG 0.517255 1:-60147041 None:intergenic
TGTTGAACTAGCAAAAGAAA+TGG 0.525403 1:+60144530 MsG0180003294.01.T01:CDS
CCTAACCAATTCCTTGGCTG+TGG 0.525618 1:-60148516 None:intergenic
TCTACTGTGGTAGCAGTTCA+GGG 0.526335 1:+60144481 MsG0180003294.01.T01:CDS
TAATCCCCTAACCAATTCCT+TGG 0.543230 1:-60148522 None:intergenic
ACTTGGGATCCACAGCAAAC+CGG 0.543825 1:+60147078 MsG0180003294.01.T01:CDS
TCTTATATAAGATCATCATA+TGG 0.552847 1:-60148830 None:intergenic
CTGTATCTGATATGCATCAA+AGG 0.557918 1:+60146593 MsG0180003294.01.T01:CDS
GTTTCTCAAGCAGTTCATGA+TGG 0.562470 1:+60145474 MsG0180003294.01.T01:CDS
TGGTTCTTACCGGTTTGCTG+TGG 0.572186 1:-60147087 None:intergenic
AAAGGAAAGCTGAAATGGCT+AGG 0.576735 1:+60146611 MsG0180003294.01.T01:CDS
TGAAGGCTTTATTTCCCCAA+AGG 0.581047 1:+60148469 MsG0180003294.01.T01:CDS
GTGGGTCTATTGAATGTGGA+TGG 0.585448 1:+60148404 MsG0180003294.01.T01:CDS
GGAAATAAAGCCTTCATCAA+CGG 0.588345 1:-60148462 None:intergenic
CCAAGATTTCATACCAGGAA+GGG 0.596878 1:-60146653 None:intergenic
GATAATGCGACGTGCCTTTG+GGG 0.597193 1:-60148483 None:intergenic
ATTGTTGTGTTTCATTGACA+AGG 0.598135 1:+60148439 MsG0180003294.01.T01:CDS
CGTGTCAGCATCCACAGCCA+AGG 0.598380 1:+60148505 MsG0180003294.01.T01:CDS
GCCAAGATTTCATACCAGGA+AGG 0.602177 1:-60146654 None:intergenic
GCAGGTGGGTCTATTGAATG+TGG 0.607073 1:+60148400 MsG0180003294.01.T01:intron
AGATGAATATGTATCCAAGT+TGG 0.616544 1:+60148765 MsG0180003294.01.T01:CDS
AAGAAACTAACCCAAGAACA+TGG 0.618263 1:-60145503 None:intergenic
GATAACTGGTGACCCTATTG+GGG 0.618479 1:+60146561 MsG0180003294.01.T01:intron
AATATGTATCCAAGTTGGTG+TGG 0.623210 1:+60148770 MsG0180003294.01.T01:CDS
ACAGCCAAGGAATTGGTTAG+GGG 0.625582 1:+60148518 MsG0180003294.01.T01:CDS
CTCTTGGCATGAGACTCCTA+GGG 0.627464 1:-60146412 None:intergenic
AAGAGGATTTGTGTCTACTG+TGG 0.635693 1:+60144468 MsG0180003294.01.T01:CDS
TCTCAAGCAGTTCATGATGG+CGG 0.641857 1:+60145477 MsG0180003294.01.T01:CDS
TAGGAGTCTCATGCCAAGAG+AGG 0.642075 1:+60146415 MsG0180003294.01.T01:CDS
ATATGTATCCAAGTTGGTGT+GGG 0.680600 1:+60148771 MsG0180003294.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATAAAATTAAAAAATTT+TGG - Chr1:60145208-60145227 None:intergenic 0.0%
!!! AAATAAAATTAAAAAATTTT+GGG - Chr1:60145207-60145226 None:intergenic 0.0%
!!! TTTTATATTTTTATATTTTA+TGG + Chr1:60144670-60144689 MsG0180003294.01.T01:intron 0.0%
!! GAAAAAATAAAAATAAACAA+AGG - Chr1:60144810-60144829 None:intergenic 10.0%
!! GATGTAAAAAAAAAAAAAAA+AGG - Chr1:60145185-60145204 None:intergenic 10.0%
!! TATAAATTTACTATATAATG+TGG + Chr1:60145980-60145999 MsG0180003294.01.T01:intron 10.0%
!!! ATGTAAAAAAATACATTTTT+TGG - Chr1:60144646-60144665 None:intergenic 10.0%
!!! TTAAATTTTACTTGTATTTT+AGG - Chr1:60147153-60147172 None:intergenic 10.0%
!!! TTGATTGTTTAATATTTTTA+TGG + Chr1:60148376-60148395 MsG0180003294.01.T01:intron 10.0%
!! AAAGAAAACATCTTTATTAT+AGG - Chr1:60144578-60144597 None:intergenic 15.0%
!! ATAAATAAAAAAGAGTAGTA+TGG - Chr1:60147110-60147129 None:intergenic 15.0%
!! ATTGAAATCATTAATTTCTT+AGG - Chr1:60145838-60145857 None:intergenic 15.0%
!! TTGAAATCATTAATTTCTTA+GGG - Chr1:60145837-60145856 None:intergenic 15.0%
!! TTTGTTAATAAAATATATGG+TGG + Chr1:60146527-60146546 MsG0180003294.01.T01:intron 15.0%
!!! AAAAAAATACATTTTTTGGA+AGG - Chr1:60144642-60144661 None:intergenic 15.0%
!!! AACTTCTGTATTTTAAAAAT+AGG + Chr1:60145572-60145591 MsG0180003294.01.T01:intron 15.0%
!!! ACAAGACAAAAATATTTTAA+TGG - Chr1:60146341-60146360 None:intergenic 15.0%
!!! CTAGTTTGTTTAATTATAAA+AGG - Chr1:60147466-60147485 None:intergenic 15.0%
!! ACTATATAATGTGGTTAATT+TGG + Chr1:60145989-60146008 MsG0180003294.01.T01:intron 20.0%
!! ATAAAATATTAGTAGAGTGT+GGG + Chr1:60145878-60145897 MsG0180003294.01.T01:intron 20.0%
!! ATTGTTGAGTTTATATAAAG+CGG + Chr1:60146023-60146042 MsG0180003294.01.T01:intron 20.0%
!! ATTTCAATGTAAGACATTAT+GGG + Chr1:60145850-60145869 MsG0180003294.01.T01:intron 20.0%
!! TAAAAATCATGAAAGATTGT+TGG - Chr1:60145144-60145163 None:intergenic 20.0%
!! TATGTCTTATGATAACAAAT+TGG - Chr1:60147264-60147283 None:intergenic 20.0%
!! TATTGAATATAAAGTGAAGA+TGG - Chr1:60148576-60148595 None:intergenic 20.0%
!! TCTACAAAAATTAATGTATC+TGG + Chr1:60147173-60147192 MsG0180003294.01.T01:intron 20.0%
!! TTATGTCTCTATTATAACTT+TGG - Chr1:60147362-60147381 None:intergenic 20.0%
!!! AAAACAATGGTTTTTAAACA+TGG - Chr1:60147692-60147711 None:intergenic 20.0%
!!! ACAAATACGTTTTAGTTTTT+TGG + Chr1:60147739-60147758 MsG0180003294.01.T01:intron 20.0%
!!! ATGTGTATAACTTTTACTTA+TGG + Chr1:60146064-60146083 MsG0180003294.01.T01:intron 20.0%
!!! ATTTTACTTGTATTTTAGGA+CGG - Chr1:60147149-60147168 None:intergenic 20.0%
!!! CATTTTTAGTAATACTTCAT+CGG + Chr1:60146738-60146757 MsG0180003294.01.T01:intron 20.0%
!!! GTAACATAAGTTGATATATT+TGG - Chr1:60147215-60147234 None:intergenic 20.0%
!!! TCATGTTCATATTTTTTTTG+TGG + Chr1:60144742-60144761 MsG0180003294.01.T01:intron 20.0%
!!! TCTTGTTATTTTGAAACATT+AGG + Chr1:60147007-60147026 MsG0180003294.01.T01:intron 20.0%
!!! TTTTATAAAGTTCCAAATCT+AGG + Chr1:60144841-60144860 MsG0180003294.01.T01:intron 20.0%
! AAACCAAATCAAAGTTTAAG+AGG + Chr1:60144451-60144470 MsG0180003294.01.T01:CDS 25.0%
! AATCCTCTTAAACTTTGATT+TGG - Chr1:60144457-60144476 None:intergenic 25.0%
! AATTGCTGGAAATTATTACT+TGG + Chr1:60147050-60147069 MsG0180003294.01.T01:CDS 25.0%
! ACATGAAAAGATAGTACTTT+AGG + Chr1:60147880-60147899 MsG0180003294.01.T01:intron 25.0%
! ATAATTGAACCAAAACATGT+GGG + Chr1:60147840-60147859 MsG0180003294.01.T01:intron 25.0%
! ATACATATTGCTAAAACAAG+TGG + Chr1:60148665-60148684 MsG0180003294.01.T01:intron 25.0%
! ATTGCTGGAAATTATTACTT+GGG + Chr1:60147051-60147070 MsG0180003294.01.T01:CDS 25.0%
! CAAAAAACCAATAAAGTTGT+TGG + Chr1:60148049-60148068 MsG0180003294.01.T01:intron 25.0%
! CATAAAATATTAGTAGAGTG+TGG + Chr1:60145877-60145896 MsG0180003294.01.T01:intron 25.0%
! CTATAACCAAAGAAGTAAAA+CGG - Chr1:60147294-60147313 None:intergenic 25.0%
! CTTAAGATACGTAAAAACAA+TGG - Chr1:60147705-60147724 None:intergenic 25.0%
! CTTTGTATCATTACACTATA+CGG + Chr1:60145093-60145112 MsG0180003294.01.T01:intron 25.0%
! GACTAATGATGTATGTTATA+TGG + Chr1:60146368-60146387 MsG0180003294.01.T01:intron 25.0%
! GATTTCAATGTAAGACATTA+TGG + Chr1:60145849-60145868 MsG0180003294.01.T01:intron 25.0%
! TAAGCATGATACATAACATT+AGG + Chr1:60147805-60147824 MsG0180003294.01.T01:intron 25.0%
! TACAATTAGAAGCTTCTATA+GGG - Chr1:60145927-60145946 None:intergenic 25.0%
! TACATATTGCTAAAACAAGT+GGG + Chr1:60148666-60148685 MsG0180003294.01.T01:intron 25.0%
! TATAATTGAACCAAAACATG+TGG + Chr1:60147839-60147858 MsG0180003294.01.T01:intron 25.0%
! TCTGTAAAAAATAAGCCAAA+AGG - Chr1:60146128-60146147 None:intergenic 25.0%
! TTACTTCTTTGGTTATAGTT+GGG + Chr1:60147296-60147315 MsG0180003294.01.T01:intron 25.0%
! TTGTTGAGTTTATATAAAGC+GGG + Chr1:60146024-60146043 MsG0180003294.01.T01:intron 25.0%
! TTTACTTCTTTGGTTATAGT+TGG + Chr1:60147295-60147314 MsG0180003294.01.T01:intron 25.0%
! TTTCAATGTAAGACATTATG+GGG + Chr1:60145851-60145870 MsG0180003294.01.T01:intron 25.0%
!! ATAGAGATTGATTGATTTAG+TGG - Chr1:60145244-60145263 None:intergenic 25.0%
!! ATTTGTGTTTTCAATGTTGA+AGG + Chr1:60145395-60145414 MsG0180003294.01.T01:intron 25.0%
!! CGATGAAGTATTACTAAAAA+TGG - Chr1:60146740-60146759 None:intergenic 25.0%
!! TTAGTAATTCACATTTTCGT+CGG + Chr1:60148199-60148218 MsG0180003294.01.T01:intron 25.0%
!! TTTATTGATTGCTAAGAAGT+GGG + Chr1:60145771-60145790 MsG0180003294.01.T01:intron 25.0%
!!! GTTTAATATTTTTATGGTGC+AGG + Chr1:60148382-60148401 MsG0180003294.01.T01:intron 25.0%
!!! TGTTATTTTGAAACATTAGG+TGG + Chr1:60147010-60147029 MsG0180003294.01.T01:intron 25.0%
!!! TTTTATTGATTGCTAAGAAG+TGG + Chr1:60145770-60145789 MsG0180003294.01.T01:intron 25.0%
!!! TTTTGAAGACATTGGTTAAA+AGG + Chr1:60146096-60146115 MsG0180003294.01.T01:intron 25.0%
AAAAAAAAAAAGGTAGCCCT+TGG - Chr1:60145175-60145194 None:intergenic 30.0%
AAAACTAAGATCATGCAAGA+AGG - Chr1:60144934-60144953 None:intergenic 30.0%
AAAGGAAAAGGCAAAAACAT+TGG - Chr1:60146174-60146193 None:intergenic 30.0%
AAGATTGTTGGAAATGGTAA+AGG - Chr1:60145132-60145151 None:intergenic 30.0%
AATCTAGGTTAAGATTGTTG+AGG + Chr1:60144856-60144875 MsG0180003294.01.T01:intron 30.0%
AATGTATGAAGCCTCTAATA+TGG - Chr1:60146866-60146885 None:intergenic 30.0%
AATTTCCAGCAATTCTTCAA+GGG - Chr1:60147044-60147063 None:intergenic 30.0%
ACAATTAGAAGCTTCTATAG+GGG - Chr1:60145926-60145945 None:intergenic 30.0%
AGATGAATATGTATCCAAGT+TGG + Chr1:60148765-60148784 MsG0180003294.01.T01:CDS 30.0%
AGGATATTATCATGTGAAGT+GGG + Chr1:60146944-60146963 MsG0180003294.01.T01:intron 30.0%
ATAAACAAAGGAGAAGAAAG+AGG - Chr1:60144798-60144817 None:intergenic 30.0%
ATATCTTTCTCCCATTCAAA+TGG - Chr1:60147976-60147995 None:intergenic 30.0%
ATTTGGTTAACACATGAAGT+TGG + Chr1:60146974-60146993 MsG0180003294.01.T01:intron 30.0%
CAACAATCTTAACCTAGATT+TGG - Chr1:60144856-60144875 None:intergenic 30.0%
CATTTAATGTTATGTGAAGC+AGG + Chr1:60148726-60148745 MsG0180003294.01.T01:intron 30.0%
CTACAATTAGAAGCTTCTAT+AGG - Chr1:60145928-60145947 None:intergenic 30.0%
CTACACTAGTAAAACATGAA+AGG + Chr1:60146676-60146695 MsG0180003294.01.T01:intron 30.0%
CTATAGAAGCTTCTAATTGT+AGG + Chr1:60145926-60145945 MsG0180003294.01.T01:intron 30.0%
CTTCTAATTGTAGGATGTAA+GGG + Chr1:60145935-60145954 MsG0180003294.01.T01:intron 30.0%
GATATATGACGTTAGATTGA+TGG + Chr1:60147946-60147965 MsG0180003294.01.T01:intron 30.0%
GTCTATATCTATGTGTTTGT+TGG + Chr1:60145727-60145746 MsG0180003294.01.T01:intron 30.0%
GTTGAAAATACACACAATCT+AGG + Chr1:60145640-60145659 MsG0180003294.01.T01:intron 30.0%
TAATTGAACCAAAACATGTG+GGG + Chr1:60147841-60147860 MsG0180003294.01.T01:intron 30.0%
TAATTTCCAGCAATTCTTCA+AGG - Chr1:60147045-60147064 None:intergenic 30.0%
TACTTCTTTGGTTATAGTTG+GGG + Chr1:60147297-60147316 MsG0180003294.01.T01:intron 30.0%
TAGGATATTATCATGTGAAG+TGG + Chr1:60146943-60146962 MsG0180003294.01.T01:intron 30.0%
TCATGAAAGATTGTTGGAAA+TGG - Chr1:60145138-60145157 None:intergenic 30.0%
TCATTAATTTCTTAGGGAAC+AGG - Chr1:60145831-60145850 None:intergenic 30.0%
TGTTGAACTAGCAAAAGAAA+TGG + Chr1:60144530-60144549 MsG0180003294.01.T01:CDS 30.0%
TGTTGAGTTTATATAAAGCG+GGG + Chr1:60146025-60146044 MsG0180003294.01.T01:intron 30.0%
TTCAATGTAAGACATTATGG+GGG + Chr1:60145852-60145871 MsG0180003294.01.T01:intron 30.0%
! TTTGAAACATTAGGTGGATA+TGG + Chr1:60147016-60147035 MsG0180003294.01.T01:intron 30.0%
!! AATATTTTTATGGTGCAGGT+GGG + Chr1:60148386-60148405 MsG0180003294.01.T01:intron 30.0%
!! AGAAGGATTGATTTGGTTTA+TGG + Chr1:60145423-60145442 MsG0180003294.01.T01:CDS 30.0%
!! ATTTTCAACTAGAGTTCTGA+TGG - Chr1:60145629-60145648 None:intergenic 30.0%
!! TAATATTTTTATGGTGCAGG+TGG + Chr1:60148385-60148404 MsG0180003294.01.T01:intron 30.0%
!! TAGAAGCTTTTTGAAGACAT+TGG + Chr1:60146088-60146107 MsG0180003294.01.T01:intron 30.0%
!! TTGTTACTTTCTTTCTTGCT+TGG + Chr1:60145327-60145346 MsG0180003294.01.T01:intron 30.0%
!! TTTTCAACTAGAGTTCTGAT+GGG - Chr1:60145628-60145647 None:intergenic 30.0%
!!! AGATTGTTTTATTTGCTAGC+TGG + Chr1:60144886-60144905 MsG0180003294.01.T01:intron 30.0%
!!! TATATGCCGTTTTACTTCTT+TGG + Chr1:60147285-60147304 MsG0180003294.01.T01:intron 30.0%
AAAAATGGTCAATTGACACG+TGG - Chr1:60146725-60146744 None:intergenic 35.0%
AAAATGGTCAATTGACACGT+GGG - Chr1:60146724-60146743 None:intergenic 35.0%
AAAGGACAGCATTAACTCAA+TGG - Chr1:60146445-60146464 None:intergenic 35.0%
AAGAAACTAACCCAAGAACA+TGG - Chr1:60145506-60145525 None:intergenic 35.0%
AAGAGTAGTATGGTTCTTAC+CGG - Chr1:60147100-60147119 None:intergenic 35.0%
AATTAGAAGCTTCTATAGGG+GGG - Chr1:60145924-60145943 None:intergenic 35.0%
ACTTCTTTGGTTATAGTTGG+GGG + Chr1:60147298-60147317 MsG0180003294.01.T01:intron 35.0%
ACTTTCTTTCTTGCTTGGTT+TGG + Chr1:60145332-60145351 MsG0180003294.01.T01:intron 35.0%
ATTATTACTTGGGCTCAACT+TGG + Chr1:60147061-60147080 MsG0180003294.01.T01:CDS 35.0%
CAATTAGAAGCTTCTATAGG+GGG - Chr1:60145925-60145944 None:intergenic 35.0%
CCAATAAAGTTGTTGGTCAA+TGG + Chr1:60148056-60148075 MsG0180003294.01.T01:intron 35.0%
CCATTGACCAACAACTTTAT+TGG - Chr1:60148059-60148078 None:intergenic 35.0%
CTGTATCTGATATGCATCAA+AGG + Chr1:60146593-60146612 MsG0180003294.01.T01:CDS 35.0%
CTTGGTTCAATGAAAGTCTT+TGG - Chr1:60146842-60146861 None:intergenic 35.0%
GATACATATTCATCTTGCTC+AGG - Chr1:60148760-60148779 None:intergenic 35.0%
GCTTCTAATTGTAGGATGTA+AGG + Chr1:60145934-60145953 MsG0180003294.01.T01:intron 35.0%
GGAAATAAAGCCTTCATCAA+CGG - Chr1:60148465-60148484 None:intergenic 35.0%
GTATGACATGCATCATAGAT+AGG - Chr1:60147652-60147671 None:intergenic 35.0%
GTGAAGTGGGATTCTTAATT+TGG + Chr1:60146957-60146976 MsG0180003294.01.T01:intron 35.0%
TCATATGGCAACTTCTGATT+CGG - Chr1:60148818-60148837 None:intergenic 35.0%
TGATGGTTCTCCATTTGAAT+GGG + Chr1:60147963-60147982 MsG0180003294.01.T01:intron 35.0%
TTATTACTTGGGCTCAACTT+GGG + Chr1:60147062-60147081 MsG0180003294.01.T01:CDS 35.0%
TTGAACCAAGACCATATTAG+AGG + Chr1:60146852-60146871 MsG0180003294.01.T01:intron 35.0%
! AATGCTTTCTGCACTTTTCT+AGG + Chr1:60145053-60145072 MsG0180003294.01.T01:intron 35.0%
! AGAGCAATTCACATTTTCGT+CGG + Chr1:60148117-60148136 MsG0180003294.01.T01:intron 35.0%
! ATTCAATACCCCACATGTTT+TGG - Chr1:60147852-60147871 None:intergenic 35.0%
! CTATGTGTTTGTTGGATGAA+AGG + Chr1:60145735-60145754 MsG0180003294.01.T01:intron 35.0%
! GATTTTTATCCTACAGCCAA+GGG + Chr1:60145156-60145175 MsG0180003294.01.T01:intron 35.0%
! GTTAAAAGGTGAATGCCTTT+TGG + Chr1:60146110-60146129 MsG0180003294.01.T01:intron 35.0%
! TGATTTTTATCCTACAGCCA+AGG + Chr1:60145155-60145174 MsG0180003294.01.T01:intron 35.0%
! TTGATGGTTCTCCATTTGAA+TGG + Chr1:60147962-60147981 MsG0180003294.01.T01:intron 35.0%
! TTTTACAGAAAGTTCATGCG+CGG + Chr1:60146139-60146158 MsG0180003294.01.T01:intron 35.0%
!! AATATGTATCCAAGTTGGTG+TGG + Chr1:60148770-60148789 MsG0180003294.01.T01:CDS 35.0%
!! AGGATTGATTTGGTTTATGG+AGG + Chr1:60145426-60145445 MsG0180003294.01.T01:CDS 35.0%
!! ATATGTATCCAAGTTGGTGT+GGG + Chr1:60148771-60148790 MsG0180003294.01.T01:CDS 35.0%
!! ATTGATTTGGTTTATGGAGG+TGG + Chr1:60145429-60145448 MsG0180003294.01.T01:CDS 35.0%
!!! CTTCATCGGTTTTTGTTTAC+CGG + Chr1:60146752-60146771 MsG0180003294.01.T01:intron 35.0%
AAAGGAAAGCTGAAATGGCT+AGG + Chr1:60146611-60146630 MsG0180003294.01.T01:CDS 40.0%
AAGAGGATTTGTGTCTACTG+TGG + Chr1:60144468-60144487 MsG0180003294.01.T01:CDS 40.0%
AAGTGAAGATGGTTGAGCAA+TGG - Chr1:60148565-60148584 None:intergenic 40.0%
AATTCCAGAGAGTTCACAAG+AGG - Chr1:60147501-60147520 None:intergenic 40.0%
AATTTCTTAGGGAACAGGCA+TGG - Chr1:60145826-60145845 None:intergenic 40.0%
ACTCAATGGTTTACCTCTCT+TGG - Chr1:60146431-60146450 None:intergenic 40.0%
AGAGCATACTCATGTGAAGT+AGG + Chr1:60146924-60146943 MsG0180003294.01.T01:intron 40.0%
AGATAACTGGTGACCCTATT+GGG + Chr1:60146560-60146579 MsG0180003294.01.T01:intron 40.0%
ATCCACATGTGGACCATATA+TGG + Chr1:60146785-60146804 MsG0180003294.01.T01:intron 40.0%
ATTTCTTAGGGAACAGGCAT+GGG - Chr1:60145825-60145844 None:intergenic 40.0%
CAAAGTGCATGCAAGTGAAA+AGG - Chr1:60146192-60146211 None:intergenic 40.0%
CAATGTTGAAGGTTGAGAGA+AGG + Chr1:60145406-60145425 MsG0180003294.01.T01:intron 40.0%
CATATGGCAACTTCTGATTC+GGG - Chr1:60148817-60148836 None:intergenic 40.0%
CATGTGGATCTGGTAAAATC+CGG - Chr1:60146774-60146793 None:intergenic 40.0%
CCAAGATTTCATACCAGGAA+GGG - Chr1:60146656-60146675 None:intergenic 40.0%
GATCTTATTGCAACGAGCAA+AGG - Chr1:60146463-60146482 None:intergenic 40.0%
GCATCAAAGGAAAGCTGAAA+TGG + Chr1:60146606-60146625 MsG0180003294.01.T01:CDS 40.0%
GCATGCAAGTGAAAAGGAAA+AGG - Chr1:60146186-60146205 None:intergenic 40.0%
GGTTGAGAGAAGGATTGATT+TGG + Chr1:60145416-60145435 MsG0180003294.01.T01:intron 40.0%
GTTTATGCAGTGTTATCCCT+AGG + Chr1:60146396-60146415 MsG0180003294.01.T01:intron 40.0%
GTTTCTCAAGCAGTTCATGA+TGG + Chr1:60145474-60145493 MsG0180003294.01.T01:CDS 40.0%
TAACTAGAGACATGCCTGAA+AGG + Chr1:60147401-60147420 MsG0180003294.01.T01:intron 40.0%
TAATCCCCTAACCAATTCCT+TGG - Chr1:60148525-60148544 None:intergenic 40.0%
TCATAGATAGGAGGCAAAGT+TGG - Chr1:60147640-60147659 None:intergenic 40.0%
TGAAGCCTCTAATATGGTCT+TGG - Chr1:60146860-60146879 None:intergenic 40.0%
TGACATGCATCATAGATAGG+AGG - Chr1:60147649-60147668 None:intergenic 40.0%
TGGTCGAAAATGCAGATAAC+TGG + Chr1:60146547-60146566 MsG0180003294.01.T01:intron 40.0%
TGTAGCCAAGATTTCATACC+AGG - Chr1:60146661-60146680 None:intergenic 40.0%
TTATTCCATTCCCACCTTTC+AGG - Chr1:60147418-60147437 None:intergenic 40.0%
TTTACAGAAAGTTCATGCGC+GGG + Chr1:60146140-60146159 MsG0180003294.01.T01:intron 40.0%
! ACATTTTCGTCGGTAGTCAT+CGG + Chr1:60148127-60148146 MsG0180003294.01.T01:intron 40.0%
! ATAGGAGGCAAAGTTGGTTA+AGG - Chr1:60147634-60147653 None:intergenic 40.0%
! GATTTTACCAGATCCACATG+TGG + Chr1:60146774-60146793 MsG0180003294.01.T01:intron 40.0%
!! TGAAGGCTTTATTTCCCCAA+AGG + Chr1:60148469-60148488 MsG0180003294.01.T01:CDS 40.0%
ACAGCAGCTTCTTGGTAAGT+AGG - Chr1:60144513-60144532 None:intergenic 45.0%
ACAGCCAAGGAATTGGTTAG+GGG + Chr1:60148518-60148537 MsG0180003294.01.T01:CDS 45.0%
ATATGGTCCACATGTGGATC+TGG - Chr1:60146784-60146803 None:intergenic 45.0%
CAACAACTGTTGTAGCAGAC+CGG - Chr1:60146812-60146831 None:intergenic 45.0%
CACAGCCAAGGAATTGGTTA+GGG + Chr1:60148517-60148536 MsG0180003294.01.T01:CDS 45.0%
CAGATAACTGGTGACCCTAT+TGG + Chr1:60146559-60146578 MsG0180003294.01.T01:intron 45.0%
CATGTGGACCATATATGGAC+CGG + Chr1:60146790-60146809 MsG0180003294.01.T01:intron 45.0%
CCCTTCCTGGTATGAAATCT+TGG + Chr1:60146653-60146672 MsG0180003294.01.T01:intron 45.0%
CTAGTTCAACAGCAGCTTCT+TGG - Chr1:60144521-60144540 None:intergenic 45.0%
GATAACTGGTGACCCTATTG+GGG + Chr1:60146561-60146580 MsG0180003294.01.T01:intron 45.0%
GCCAAGATTTCATACCAGGA+AGG - Chr1:60146657-60146676 None:intergenic 45.0%
GGAATTGGTTAGGGGATTAG+AGG + Chr1:60148526-60148545 MsG0180003294.01.T01:CDS 45.0%
GGGATCTGATGCATTAATGC+TGG - Chr1:60145608-60145627 None:intergenic 45.0%
GTCCATATATGGTCCACATG+TGG - Chr1:60146790-60146809 None:intergenic 45.0%
GTGGGTCTATTGAATGTGGA+TGG + Chr1:60148404-60148423 MsG0180003294.01.T01:CDS 45.0%
TAGAGACATGCCTGAAAGGT+GGG + Chr1:60147405-60147424 MsG0180003294.01.T01:intron 45.0%
TCTACTGTGGTAGCAGTTCA+GGG + Chr1:60144481-60144500 MsG0180003294.01.T01:CDS 45.0%
TCTCAAGCAGTTCATGATGG+CGG + Chr1:60145477-60145496 MsG0180003294.01.T01:CDS 45.0%
TCTTTCTTCCCACACCAACT+TGG - Chr1:60148782-60148801 None:intergenic 45.0%
TGGAACCCTTGAAGAATTGC+TGG + Chr1:60147036-60147055 MsG0180003294.01.T01:CDS 45.0%
! ATTGACAAGGCCGTTGATGA+AGG + Chr1:60148452-60148471 MsG0180003294.01.T01:CDS 45.0%
! GTTTATGGAGGTGGTAGTGT+GGG + Chr1:60145438-60145457 MsG0180003294.01.T01:CDS 45.0%
!!! TAATTTGTTAATAAAATATA+TGG + Chr1:60146524-60146543 MsG0180003294.01.T01:intron 5.0%
!!! TTTTTTTCTTTTTAATTATT+CGG + Chr1:60147598-60147617 MsG0180003294.01.T01:intron 5.0%
AAAAGGTAGCCCTTGGCTGT+AGG - Chr1:60145168-60145187 None:intergenic 50.0%
ACAGCTCTCACTTCCCCAAT+AGG - Chr1:60146577-60146596 None:intergenic 50.0%
ACATGCCTGAAAGGTGGGAA+TGG + Chr1:60147410-60147429 MsG0180003294.01.T01:intron 50.0%
ACGATAATGCGACGTGCCTT+TGG - Chr1:60148488-60148507 None:intergenic 50.0%
ACTTGGGATCCACAGCAAAC+CGG + Chr1:60147078-60147097 MsG0180003294.01.T01:CDS 50.0%
AGCATCCACAGCCAAGGAAT+TGG + Chr1:60148511-60148530 MsG0180003294.01.T01:CDS 50.0%
CAGCTCTCACTTCCCCAATA+GGG - Chr1:60146576-60146595 None:intergenic 50.0%
CATACCAGGAAGGGCAATGA+AGG - Chr1:60146647-60146666 None:intergenic 50.0%
CCACAGCCAAGGAATTGGTT+AGG + Chr1:60148516-60148535 MsG0180003294.01.T01:CDS 50.0%
CCTAACCAATTCCTTGGCTG+TGG - Chr1:60148519-60148538 None:intergenic 50.0%
CGATAATGCGACGTGCCTTT+GGG - Chr1:60148487-60148506 None:intergenic 50.0%
CTAGAGACATGCCTGAAAGG+TGG + Chr1:60147404-60147423 MsG0180003294.01.T01:intron 50.0%
CTCTTGGCATGAGACTCCTA+GGG - Chr1:60146415-60146434 None:intergenic 50.0%
CTTGCCTCTTGTGAACTCTC+TGG + Chr1:60147494-60147513 MsG0180003294.01.T01:intron 50.0%
GAAAGCTGAAATGGCTAGGC+AGG + Chr1:60146615-60146634 MsG0180003294.01.T01:CDS 50.0%
GAAAGTTCATGCGCGGGAAT+TGG + Chr1:60146146-60146165 MsG0180003294.01.T01:intron 50.0%
GATAATGCGACGTGCCTTTG+GGG - Chr1:60148486-60148505 None:intergenic 50.0%
GCAGGTGGGTCTATTGAATG+TGG + Chr1:60148400-60148419 MsG0180003294.01.T01:intron 50.0%
GTAGCAGACCGGTCCATATA+TGG - Chr1:60146801-60146820 None:intergenic 50.0%
GTCTACTGTGGTAGCAGTTC+AGG + Chr1:60144480-60144499 MsG0180003294.01.T01:CDS 50.0%
TAGGAGTCTCATGCCAAGAG+AGG + Chr1:60146415-60146434 MsG0180003294.01.T01:CDS 50.0%
TCTCTTGGCATGAGACTCCT+AGG - Chr1:60146416-60146435 None:intergenic 50.0%
! GGTTTATGGAGGTGGTAGTG+TGG + Chr1:60145437-60145456 MsG0180003294.01.T01:CDS 50.0%
!! TGGTTCTTACCGGTTTGCTG+TGG - Chr1:60147090-60147109 None:intergenic 50.0%
GATGCCTTCATTGCCCTTCC+TGG + Chr1:60146640-60146659 MsG0180003294.01.T01:CDS 55.0%
GGTCAATTGACACGTGGGCT+CGG - Chr1:60146719-60146738 None:intergenic 55.0%
! AGGTGGTAGTGTGGGTCTGA+TGG + Chr1:60145446-60145465 MsG0180003294.01.T01:CDS 55.0%
! GGTGGTAGTGTGGGTCTGAT+GGG + Chr1:60145447-60145466 MsG0180003294.01.T01:CDS 55.0%
!! ATGGCGGACGCCATGTTCTT+GGG + Chr1:60145493-60145512 MsG0180003294.01.T01:CDS 55.0%
CGTGTCAGCATCCACAGCCA+AGG + Chr1:60148505-60148524 MsG0180003294.01.T01:CDS 60.0%
!! GATGGCGGACGCCATGTTCT+TGG + Chr1:60145492-60145511 MsG0180003294.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 60144444 60148837 60144444 ID=MsG0180003294.01;Name=MsG0180003294.01
Chr1 mRNA 60144444 60148837 60144444 ID=MsG0180003294.01.T01;Parent=MsG0180003294.01;Name=MsG0180003294.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|1|1|7|0|213
Chr1 exon 60144444 60144551 60144444 ID=MsG0180003294.01.T01:exon:49239;Parent=MsG0180003294.01.T01
Chr1 exon 60145417 60145514 60145417 ID=MsG0180003294.01.T01:exon:49240;Parent=MsG0180003294.01.T01
Chr1 exon 60146406 60146436 60146406 ID=MsG0180003294.01.T01:exon:49241;Parent=MsG0180003294.01.T01
Chr1 exon 60146562 60146661 60146562 ID=MsG0180003294.01.T01:exon:49242;Parent=MsG0180003294.01.T01
Chr1 exon 60147029 60147099 60147029 ID=MsG0180003294.01.T01:exon:49243;Parent=MsG0180003294.01.T01
Chr1 exon 60148404 60148547 60148404 ID=MsG0180003294.01.T01:exon:49244;Parent=MsG0180003294.01.T01
Chr1 exon 60148748 60148837 60148748 ID=MsG0180003294.01.T01:exon:49245;Parent=MsG0180003294.01.T01
Chr1 CDS 60144444 60144551 60144444 ID=MsG0180003294.01.T01:cds;Parent=MsG0180003294.01.T01
Chr1 CDS 60145417 60145514 60145417 ID=MsG0180003294.01.T01:cds;Parent=MsG0180003294.01.T01
Chr1 CDS 60146406 60146436 60146406 ID=MsG0180003294.01.T01:cds;Parent=MsG0180003294.01.T01
Chr1 CDS 60146562 60146661 60146562 ID=MsG0180003294.01.T01:cds;Parent=MsG0180003294.01.T01
Chr1 CDS 60147029 60147099 60147029 ID=MsG0180003294.01.T01:cds;Parent=MsG0180003294.01.T01
Chr1 CDS 60148404 60148547 60148404 ID=MsG0180003294.01.T01:cds;Parent=MsG0180003294.01.T01
Chr1 CDS 60148748 60148837 60148748 ID=MsG0180003294.01.T01:cds;Parent=MsG0180003294.01.T01
Gene Sequence

>MsG0180003294.01.T01

ATGGAAGAAACCAAATCAAAGTTTAAGAGGATTTGTGTCTACTGTGGTAGCAGTTCAGGGAACAAACCTACTTACCAAGAAGCTGCTGTTGAACTAGCAAAAGAAATGGTTGAGAGAAGGATTGATTTGGTTTATGGAGGTGGTAGTGTGGGTCTGATGGGTCTTGTTTCTCAAGCAGTTCATGATGGCGGACGCCATGTTCTTGGTGTTATCCCTAGGAGTCTCATGCCAAGAGAGATAACTGGTGACCCTATTGGGGAAGTGAGAGCTGTATCTGATATGCATCAAAGGAAAGCTGAAATGGCTAGGCAGGCTGATGCCTTCATTGCCCTTCCTGGTGGATATGGAACCCTTGAAGAATTGCTGGAAATTATTACTTGGGCTCAACTTGGGATCCACAGCAAACCGGTGGGTCTATTGAATGTGGATGGATTTTACAATTCATTGTTGTGTTTCATTGACAAGGCCGTTGATGAAGGCTTTATTTCCCCAAAGGCACGTCGCATTATCGTGTCAGCATCCACAGCCAAGGAATTGGTTAGGGGATTAGAGGAACATGTACCTGAGCAAGATGAATATGTATCCAAGTTGGTGTGGGAAGAAAGACTAAATTACGTGCCCGAATCAGAAGTTGCCATATGA
Protein sequence

>MsG0180003294.01.T01

MEETKSKFKRICVYCGSSSGNKPTYQEAAVELAKEMVERRIDLVYGGGSVGLMGLVSQAVHDGGRHVLGVIPRSLMPREITGDPIGEVRAVSDMHQRKAEMARQADAFIALPGGYGTLEELLEIITWAQLGIHSKPVGLLNVDGFYNSLLCFIDKAVDEGFISPKARRIIVSASTAKELVRGLEEHVPEQDEYVSKLVWEERLNYVPESEVAI*