Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017898.01.T01 | XP_003603845.1 | 94.626 | 428 | 23 | 0 | 1 | 428 | 1 | 428 | 0 | 837 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017898.01.T01 | P50134 | 57.108 | 415 | 177 | 1 | 10 | 424 | 18 | 431 | 3.15E-173 | 494 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017898.01.T01 | G7J5X8 | 94.626 | 428 | 23 | 0 | 1 | 428 | 1 | 428 | 0.0 | 837 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048509.01 | MsG0380017898.01 | 0.816710 | 4.806845e-52 | 3.431346e-49 |
| MsG0080048652.01 | MsG0380017898.01 | 0.819386 | 1.193434e-52 | 9.169957e-50 |
| MsG0180001290.01 | MsG0380017898.01 | 0.829092 | 6.246056e-55 | 6.328020e-52 |
| MsG0180003294.01 | MsG0380017898.01 | 0.802565 | 5.281046e-49 | 2.595245e-46 |
| MsG0180003484.01 | MsG0380017898.01 | 0.822852 | 1.897398e-53 | 1.606987e-50 |
| MsG0180003504.01 | MsG0380017898.01 | 0.813137 | 2.980438e-51 | 1.930370e-48 |
| MsG0180004510.01 | MsG0380017898.01 | 0.812721 | 3.677086e-51 | 2.355485e-48 |
| MsG0180004652.01 | MsG0380017898.01 | 0.800680 | 1.286513e-48 | 6.027164e-46 |
| MsG0180005096.01 | MsG0380017898.01 | 0.808871 | 2.503099e-50 | 1.447498e-47 |
| MsG0180006026.01 | MsG0380017898.01 | 0.833374 | 5.532308e-56 | 6.359264e-53 |
| MsG0180006076.01 | MsG0380017898.01 | -0.811040 | 8.539095e-51 | 5.230820e-48 |
| MsG0180006182.01 | MsG0380017898.01 | 0.848058 | 7.805140e-60 | 1.414716e-56 |
| MsG0180006191.01 | MsG0380017898.01 | 0.885301 | 9.150120e-72 | 6.247430e-68 |
| MsG0380013041.01 | MsG0380017898.01 | 0.880034 | 7.648250e-70 | 4.244101e-66 |
| MsG0380013415.01 | MsG0380017898.01 | 0.814896 | 1.219667e-51 | 8.285187e-49 |
| MsG0380015858.01 | MsG0380017898.01 | 0.807196 | 5.688008e-50 | 3.148302e-47 |
| MsG0380016110.01 | MsG0380017898.01 | 0.816515 | 5.314872e-52 | 3.773916e-49 |
| MsG0380016112.01 | MsG0380017898.01 | 0.802990 | 4.315992e-49 | 2.144022e-46 |
| MsG0380016485.01 | MsG0380017898.01 | 0.848630 | 5.422482e-60 | 1.001253e-56 |
| MsG0380017024.01 | MsG0380017898.01 | 0.813930 | 1.994837e-51 | 1.320147e-48 |
| MsG0380017030.01 | MsG0380017898.01 | 0.805171 | 1.518143e-49 | 7.977032e-47 |
| MsG0380017898.01 | MsG0380017901.01 | 0.812240 | 4.683928e-51 | 2.962340e-48 |
| MsG0380017898.01 | MsG0380017992.01 | 0.821795 | 3.339598e-53 | 2.744495e-50 |
| MsG0380017898.01 | MsG0380018004.01 | 0.833242 | 5.967714e-56 | 6.832397e-53 |
| MsG0380017898.01 | MsG0480018767.01 | 0.830634 | 2.629567e-55 | 2.786397e-52 |
| MsG0380017898.01 | MsG0480019935.01 | 0.812043 | 5.169127e-51 | 3.251512e-48 |
| MsG0380017898.01 | MsG0480021433.01 | 0.804955 | 1.684901e-49 | 8.804531e-47 |
| MsG0380017898.01 | MsG0480023195.01 | 0.816112 | 6.539738e-52 | 4.592946e-49 |
| MsG0380017898.01 | MsG0480023322.01 | 0.850805 | 1.339042e-60 | 2.654839e-57 |
| MsG0380017898.01 | MsG0480023944.01 | 0.846457 | 2.146297e-59 | 3.696912e-56 |
| MsG0380017898.01 | MsG0580024431.01 | 0.803865 | 2.843245e-49 | 1.444610e-46 |
| MsG0380017898.01 | MsG0580025153.01 | -0.816620 | 5.034599e-52 | 3.585257e-49 |
| MsG0380017898.01 | MsG0580025464.01 | 0.807152 | 5.811011e-50 | 3.212662e-47 |
| MsG0380017898.01 | MsG0580025633.01 | 0.825395 | 4.799183e-54 | 4.368842e-51 |
| MsG0380017898.01 | MsG0580025822.01 | 0.801222 | 9.968983e-49 | 4.734935e-46 |
| MsG0380017898.01 | MsG0580026027.01 | 0.822472 | 2.326497e-53 | 1.949179e-50 |
| MsG0380017898.01 | MsG0580026311.01 | 0.834628 | 2.685720e-56 | 3.205284e-53 |
| MsG0380017898.01 | MsG0580028302.01 | 0.819466 | 1.144547e-52 | 8.814876e-50 |
| MsG0380017898.01 | MsG0580028565.01 | 0.816047 | 6.764559e-52 | 4.742453e-49 |
| MsG0380017898.01 | MsG0580029240.01 | 0.800370 | 1.487965e-48 | 6.916647e-46 |
| MsG0380017898.01 | MsG0580029696.01 | 0.809766 | 1.608441e-50 | 9.526836e-48 |
| MsG0380017898.01 | MsG0580029751.01 | -0.807061 | 6.075588e-50 | 3.350970e-47 |
| MsG0380017898.01 | MsG0580030179.01 | 0.818208 | 2.209541e-52 | 1.643441e-49 |
| MsG0380017898.01 | MsG0680031463.01 | 0.818122 | 2.310677e-52 | 1.714624e-49 |
| MsG0380017898.01 | MsG0680034415.01 | 0.839225 | 1.799324e-57 | 2.468705e-54 |
| MsG0380017898.01 | MsG0680034417.01 | 0.821099 | 4.833930e-53 | 3.895956e-50 |
| MsG0380017898.01 | MsG0780037148.01 | 0.816459 | 5.470606e-52 | 3.878368e-49 |
| MsG0380017898.01 | MsG0780037982.01 | -0.806115 | 9.620149e-50 | 5.178816e-47 |
| MsG0380017898.01 | MsG0780038307.01 | 0.813802 | 2.128826e-51 | 1.404001e-48 |
| MsG0380017898.01 | MsG0780038393.01 | 0.800492 | 1.405281e-48 | 6.552300e-46 |
| MsG0380017898.01 | MsG0780038810.01 | 0.877124 | 8.083611e-69 | 4.018854e-65 |
| MsG0380017898.01 | MsG0780039916.01 | 0.882783 | 7.795362e-71 | 4.813309e-67 |
| MsG0380017898.01 | MsG0780039947.01 | 0.833228 | 6.017390e-56 | 6.886314e-53 |
| MsG0380017898.01 | MsG0780040021.01 | 0.806812 | 6.857870e-50 | 3.758277e-47 |
| MsG0380017898.01 | MsG0780040118.01 | 0.825591 | 4.312244e-54 | 3.947680e-51 |
| MsG0380017898.01 | MsG0780040139.01 | 0.811063 | 8.444758e-51 | 5.175984e-48 |
| MsG0380017898.01 | MsG0780040560.01 | 0.853932 | 1.722369e-61 | 3.783085e-58 |
| MsG0380017898.01 | MsG0780041100.01 | 0.806186 | 9.295677e-50 | 5.013325e-47 |
| MsG0380017898.01 | MsG0780041180.01 | 0.848110 | 7.552708e-60 | 1.371225e-56 |
| MsG0380017898.01 | MsG0780041303.01 | 0.809569 | 1.773429e-50 | 1.044898e-47 |
| MsG0380017898.01 | MsG0880042122.01 | 0.806524 | 7.889725e-50 | 4.291694e-47 |
| MsG0380017898.01 | MsG0880042433.01 | 0.801119 | 1.046373e-48 | 4.957245e-46 |
| MsG0380017898.01 | MsG0880042541.01 | 0.850545 | 1.584797e-60 | 3.115082e-57 |
| MsG0380017898.01 | MsG0880042633.01 | 0.818156 | 2.270993e-52 | 1.686720e-49 |
| MsG0380017898.01 | MsG0880042635.01 | 0.838921 | 2.157288e-57 | 2.932211e-54 |
| MsG0380017898.01 | MsG0880043953.01 | 0.813029 | 3.148183e-51 | 2.033115e-48 |
| MsG0380017898.01 | MsG0880044688.01 | 0.852055 | 5.934518e-61 | 1.224984e-57 |
| MsG0380017898.01 | MsG0880045439.01 | 0.810490 | 1.123296e-50 | 6.780367e-48 |
| MsG0380017898.01 | MsG0880045503.01 | 0.854911 | 8.979773e-62 | 2.037031e-58 |
| MsG0380017898.01 | MsG0880045584.01 | 0.805354 | 1.390013e-49 | 7.338156e-47 |
| MsG0380017898.01 | MsG0880045703.01 | -0.804345 | 2.258628e-49 | 1.161784e-46 |
| MsG0380017898.01 | MsG0880045802.01 | 0.835644 | 1.488259e-56 | 1.831136e-53 |
| MsG0380017898.01 | MsG0880045852.01 | 0.825391 | 4.807929e-54 | 4.376464e-51 |
| MsG0380017898.01 | MsG0880045854.01 | 0.844527 | 7.156373e-59 | 1.158619e-55 |
| MsG0380017898.01 | MsG0880045856.01 | 0.827325 | 1.664746e-54 | 1.602028e-51 |
| MsG0380017898.01 | MsG0880045963.01 | 0.818525 | 1.872755e-52 | 1.405254e-49 |
| MsG0380017898.01 | MsG0880046137.01 | 0.847084 | 1.446412e-59 | 2.542063e-56 |
| MsG0380017898.01 | MsG0880046337.01 | 0.829293 | 5.581969e-55 | 5.689942e-52 |
| MsG0380017898.01 | MsG0880046693.01 | 0.821303 | 4.337941e-53 | 3.515899e-50 |
| MsG0380017898.01 | MsG0880046945.01 | 0.862961 | 3.497575e-64 | 1.043000e-60 |
| MsG0380017898.01 | MsG0880047057.01 | 0.858242 | 9.423288e-63 | 2.389093e-59 |
| MsG0380017898.01 | MsG0880047450.01 | 0.828278 | 9.824478e-55 | 9.719686e-52 |
| MsG0380017898.01 | MsG0880047714.01 | 0.810289 | 1.241062e-50 | 7.451405e-48 |
| MsG0280007199.01 | MsG0380017898.01 | 0.816755 | 4.696110e-52 | 3.356574e-49 |
| MsG0280007281.01 | MsG0380017898.01 | 0.842624 | 2.307706e-58 | 3.519090e-55 |
| MsG0280007667.01 | MsG0380017898.01 | 0.801233 | 9.919853e-49 | 4.712983e-46 |
| MsG0280007786.01 | MsG0380017898.01 | 0.805629 | 1.217347e-49 | 6.472108e-47 |
| MsG0280008226.01 | MsG0380017898.01 | 0.820442 | 6.846218e-53 | 5.417730e-50 |
| MsG0280008407.01 | MsG0380017898.01 | 0.808367 | 3.206726e-50 | 1.830218e-47 |
| MsG0280009526.01 | MsG0380017898.01 | 0.805887 | 1.074348e-49 | 5.749916e-47 |
| MsG0280010240.01 | MsG0380017898.01 | 0.814913 | 1.209224e-51 | 8.218031e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017898.01.T01 | MTR_3g114870 | 94.626 | 428 | 23 | 0 | 1 | 428 | 1 | 428 | 0.0 | 837 |
| MsG0380017898.01.T01 | MTR_3g114890 | 73.223 | 422 | 82 | 4 | 1 | 422 | 38 | 428 | 0.0 | 609 |
| MsG0380017898.01.T01 | MTR_5g022700 | 25.148 | 338 | 229 | 9 | 84 | 407 | 121 | 448 | 2.79e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017898.01.T01 | AT3G14390 | 24.709 | 344 | 223 | 8 | 84 | 407 | 122 | 449 | 3.59e-14 | 74.7 |
| MsG0380017898.01.T01 | AT5G11880 | 25.581 | 344 | 220 | 9 | 84 | 407 | 127 | 454 | 3.84e-14 | 74.7 |
Find 102 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTAGCGTTCTTGATTTA+AGG | 0.113656 | 3:-101993600 | MsG0380017898.01.T01:CDS |
| TGGGCTTCAAGTTGGTAATT+TGG | 0.149477 | 3:+101993737 | None:intergenic |
| TTGGCTTGTGTTTCCAAATA+TGG | 0.194285 | 3:-101992606 | MsG0380017898.01.T01:CDS |
| AACTTCTTTGGCCAATAATA+TGG | 0.232030 | 3:+101993081 | None:intergenic |
| GTTGTATATGCACCCATATT+TGG | 0.244783 | 3:+101992593 | None:intergenic |
| CGGAGGAGGAGCTCGCATTT+TGG | 0.257621 | 3:+101993277 | None:intergenic |
| CAGTGGAGCCCGTTCGTGTC+TGG | 0.263365 | 3:-101993236 | MsG0380017898.01.T01:CDS |
| GATGAGGATCTTGTTGTTAT+TGG | 0.264779 | 3:-101992926 | MsG0380017898.01.T01:CDS |
| GTGCCTATAAGTGAAATATT+AGG | 0.265004 | 3:+101993502 | None:intergenic |
| AACCCTAATATTTCACTTAT+AGG | 0.265351 | 3:-101993505 | MsG0380017898.01.T01:CDS |
| AAAATGAAAATTCTTGATAT+TGG | 0.275753 | 3:-101993028 | MsG0380017898.01.T01:CDS |
| GGGCTTCAAGTTGGTAATTT+GGG | 0.276695 | 3:+101993738 | None:intergenic |
| TTACACTTAACGGCATAAAA+AGG | 0.284731 | 3:+101993526 | None:intergenic |
| GGAGCTAAACCCATTGAAAT+TGG | 0.285597 | 3:+101992559 | None:intergenic |
| ATTGGTGGTGGATTCACTTC+TGG | 0.295664 | 3:-101993010 | MsG0380017898.01.T01:CDS |
| ACGTTAGCAACAAAAGTTAT+TGG | 0.304090 | 3:-101992869 | MsG0380017898.01.T01:CDS |
| TGGATTGATGATGGAATTTA+CGG | 0.308997 | 3:-101992815 | MsG0380017898.01.T01:CDS |
| GGTGCATATACAACTTCTTC+TGG | 0.309142 | 3:-101992584 | MsG0380017898.01.T01:CDS |
| CGTTAGCAACAAAAGTTATT+GGG | 0.309406 | 3:-101992868 | MsG0380017898.01.T01:CDS |
| ACGGCTGGCGCAGTCGAAAT+TGG | 0.325984 | 3:+101993462 | None:intergenic |
| CTTAAATCAAGAACGCTAAA+TGG | 0.327499 | 3:+101993601 | None:intergenic |
| GTAGCTCGGGAACTTCATTT+AGG | 0.329117 | 3:+101993194 | None:intergenic |
| TTACCACGGAGCCATATTAT+TGG | 0.335936 | 3:-101993092 | MsG0380017898.01.T01:CDS |
| TCGTGTCTGGGTCTCAAATA+TGG | 0.342326 | 3:-101993223 | MsG0380017898.01.T01:CDS |
| TGTTCAACTCCAACTCTTCT+CGG | 0.361215 | 3:-101992512 | MsG0380017898.01.T01:CDS |
| TTGTGATCCATTTGTTCATA+AGG | 0.365764 | 3:+101993566 | None:intergenic |
| CAATCGTTCACCTCAAGTTC+AGG | 0.366769 | 3:+101992626 | None:intergenic |
| TCTCTCCATGCAACATTCTC+AGG | 0.369816 | 3:+101992725 | None:intergenic |
| TCTTCTGGAACCAATTTCAA+TGG | 0.383904 | 3:-101992569 | MsG0380017898.01.T01:CDS |
| GAATGCAGCTTCATCAAATC+TGG | 0.392102 | 3:+101992985 | None:intergenic |
| GGATAAAACAGATTCAATTT+CGG | 0.393834 | 3:+101993438 | None:intergenic |
| TGCCTATAAGTGAAATATTA+GGG | 0.397312 | 3:+101993503 | None:intergenic |
| ATTTAGCGTTCTTGATTTAA+GGG | 0.400399 | 3:-101993599 | MsG0380017898.01.T01:CDS |
| AATATTAGGGTTACACTTAA+CGG | 0.401795 | 3:+101993516 | None:intergenic |
| AATTTGGGATTGAGATTCTT+CGG | 0.411334 | 3:+101993753 | None:intergenic |
| AGATTCAATTTCGGCACGGC+TGG | 0.428229 | 3:+101993447 | None:intergenic |
| AGTGGAGCCCGTTCGTGTCT+GGG | 0.428327 | 3:-101993235 | MsG0380017898.01.T01:CDS |
| CTTGTTGTTATTGGTGAACC+TGG | 0.430037 | 3:-101992917 | MsG0380017898.01.T01:CDS |
| AAATGCATGCCACTTAGGTT+TGG | 0.433043 | 3:-101992755 | MsG0380017898.01.T01:CDS |
| CTTCTGGAACCAATTTCAAT+GGG | 0.439459 | 3:-101992568 | MsG0380017898.01.T01:CDS |
| TGGTCGTTACTTTGCGGAAA+CGG | 0.440992 | 3:-101992897 | MsG0380017898.01.T01:CDS |
| TGGCTTGTGTTTCCAAATAT+GGG | 0.454466 | 3:-101992605 | MsG0380017898.01.T01:CDS |
| TGTCTTCCTCCGGTTTAATA+CGG | 0.456200 | 3:+101993257 | None:intergenic |
| TGAACCTGGTCGTTACTTTG+CGG | 0.463370 | 3:-101992903 | MsG0380017898.01.T01:CDS |
| TTTGAAACAGCTTCACAACT+TGG | 0.466293 | 3:-101993058 | MsG0380017898.01.T01:CDS |
| TTGTCAAATGCATGCCACTT+AGG | 0.471188 | 3:-101992760 | MsG0380017898.01.T01:CDS |
| CACTTGTGATTCAGTTGATA+CGG | 0.472833 | 3:-101992669 | MsG0380017898.01.T01:CDS |
| AAAATTCTTGATATTGGTGG+TGG | 0.474122 | 3:-101993022 | MsG0380017898.01.T01:CDS |
| CTCAAAGCTGCTGATGTGGC+TGG | 0.475343 | 3:-101993172 | MsG0380017898.01.T01:CDS |
| GTGGCTGGACTTAAAGTAAC+CGG | 0.477454 | 3:-101993157 | MsG0380017898.01.T01:CDS |
| TTTGAGACCCAGACACGAAC+GGG | 0.477466 | 3:+101993228 | None:intergenic |
| TAATTGACCTTATGAACAAA+TGG | 0.478395 | 3:-101993573 | MsG0380017898.01.T01:CDS |
| AACATTTACGCCAACACTTG+CGG | 0.488438 | 3:+101993348 | None:intergenic |
| GATTCTTCGGTTACTAGTGT+AGG | 0.490410 | 3:+101993766 | None:intergenic |
| GTTACAACTTTCGACTGTGT+TGG | 0.493514 | 3:-101993328 | MsG0380017898.01.T01:CDS |
| TCCTAAAACTTATGCTTCAA+CGG | 0.496112 | 3:-101992702 | MsG0380017898.01.T01:CDS |
| CGGCTCCTCCACTGCCTATG+TGG | 0.498519 | 3:+101993125 | None:intergenic |
| ATCAGCAGCTTTGAGTAGCT+CGG | 0.500239 | 3:+101993180 | None:intergenic |
| GTTCAACTCCAACTCTTCTC+GGG | 0.508275 | 3:-101992511 | MsG0380017898.01.T01:CDS |
| GCTCCTCCTCCGTATTAAAC+CGG | 0.513408 | 3:-101993266 | MsG0380017898.01.T01:CDS |
| CGGGCTCCACTGTCTTCCTC+CGG | 0.514177 | 3:+101993247 | None:intergenic |
| ATGAAAATTCTTGATATTGG+TGG | 0.524512 | 3:-101993025 | MsG0380017898.01.T01:CDS |
| GTTTCTTTCCACATAGGCAG+TGG | 0.526020 | 3:-101993133 | MsG0380017898.01.T01:CDS |
| TTTGGTGCTTCAGGAGTGAA+AGG | 0.530590 | 3:-101993712 | MsG0380017898.01.T01:CDS |
| CTGAACTTGAGGTGAACGAT+TGG | 0.531047 | 3:-101992625 | MsG0380017898.01.T01:CDS |
| GAGGTGAAATGAGAGAGTAT+TGG | 0.532786 | 3:-101992835 | MsG0380017898.01.T01:CDS |
| TTCTGTCGGGTGAAACGCCA+AGG | 0.534531 | 3:+101993416 | None:intergenic |
| TCAGCAGCTTTGAGTAGCTC+GGG | 0.534584 | 3:+101993181 | None:intergenic |
| ATGTGTGACTCTGATTTACA+CGG | 0.535909 | 3:+101993379 | None:intergenic |
| AGAGAGTATTGGATTGATGA+TGG | 0.547610 | 3:-101992824 | MsG0380017898.01.T01:CDS |
| TTGGCGTAAATGATTCTGTC+GGG | 0.548085 | 3:+101993403 | None:intergenic |
| AGTTAACGCCCGAGAAGAGT+TGG | 0.549173 | 3:+101992503 | None:intergenic |
| GTTGGCGTAAATGATTCTGT+CGG | 0.557662 | 3:+101993402 | None:intergenic |
| CCTCCGGTTTAATACGGAGG+AGG | 0.565365 | 3:+101993263 | None:intergenic |
| TGACTCTGATTTACACGGGT+TGG | 0.567636 | 3:+101993384 | None:intergenic |
| ATGGATCACAAAACTTCCTA+CGG | 0.580901 | 3:-101993554 | MsG0380017898.01.T01:CDS |
| GCTACTCAAAGCTGCTGATG+TGG | 0.584654 | 3:-101993176 | MsG0380017898.01.T01:CDS |
| TCTTTCCACATAGGCAGTGG+AGG | 0.589805 | 3:-101993130 | MsG0380017898.01.T01:CDS |
| ATTAAGTACGCCGCAAGTGT+TGG | 0.598432 | 3:-101993358 | MsG0380017898.01.T01:CDS |
| GTATGAGTTACCTGAACTTG+AGG | 0.598744 | 3:-101992636 | MsG0380017898.01.T01:CDS |
| GCCTATGTGGAAAGAAACGC+CGG | 0.604972 | 3:+101993138 | None:intergenic |
| AAGTCGAAAAGATCAAAAGA+TGG | 0.607083 | 3:-101993303 | MsG0380017898.01.T01:CDS |
| ATTTGAGACCCAGACACGAA+CGG | 0.615486 | 3:+101993227 | None:intergenic |
| AACACATTTCGGAAATGATG+AGG | 0.619194 | 3:-101992942 | MsG0380017898.01.T01:CDS |
| CGTATCAACTGAATCACAAG+TGG | 0.619653 | 3:+101992670 | None:intergenic |
| CTTCCTCCGGTTTAATACGG+AGG | 0.619904 | 3:+101993260 | None:intergenic |
| GTTTCCGCAAAGTAACGACC+AGG | 0.632820 | 3:+101992899 | None:intergenic |
| AGCGACAACTTTGTCCTCAG+AGG | 0.634115 | 3:-101993683 | MsG0380017898.01.T01:CDS |
| GTTATTGGGAAACGTGTGAG+AGG | 0.635688 | 3:-101992854 | MsG0380017898.01.T01:CDS |
| AAACAGATTCAATTTCGGCA+CGG | 0.636104 | 3:+101993443 | None:intergenic |
| ACCGGCGTTTCTTTCCACAT+AGG | 0.637950 | 3:-101993139 | MsG0380017898.01.T01:CDS |
| TGTGTGACTCTGATTTACAC+GGG | 0.640940 | 3:+101993380 | None:intergenic |
| CCTCCTCCGTATTAAACCGG+AGG | 0.647197 | 3:-101993263 | MsG0380017898.01.T01:CDS |
| CAAAACCTGAGAATGTTGCA+TGG | 0.652437 | 3:-101992730 | MsG0380017898.01.T01:CDS |
| ATAGGCACACTAGCTTCACT+CGG | 0.663733 | 3:-101993487 | MsG0380017898.01.T01:CDS |
| TCTGTCGGGTGAAACGCCAA+GGG | 0.670155 | 3:+101993417 | None:intergenic |
| ATTAAACCGGAGGAAGACAG+TGG | 0.674532 | 3:-101993253 | MsG0380017898.01.T01:CDS |
| GCCGACGCAAAAGCTTACCA+CGG | 0.696059 | 3:-101993106 | MsG0380017898.01.T01:CDS |
| AATGTAAATATGCTTAGCTG+TGG | 0.700126 | 3:+101992538 | None:intergenic |
| ATCTGAGATAGTAACCTCTG+AGG | 0.725873 | 3:+101993669 | None:intergenic |
| TGGCCAATAATATGGCTCCG+TGG | 0.745513 | 3:+101993089 | None:intergenic |
| GTATCAACTGAATCACAAGT+GGG | 0.753190 | 3:+101992671 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATGAAAATTCTTGATAT+TGG | - | Chr3:101993246-101993265 | MsG0380017898.01.T01:CDS | 15.0% |
| !!! | ATATCAAGAATTTTCATTTT+TGG | + | Chr3:101993246-101993265 | None:intergenic | 15.0% |
| ! | AACCCTAATATTTCACTTAT+AGG | - | Chr3:101992769-101992788 | MsG0380017898.01.T01:CDS | 25.0% |
| ! | AATATTAGGGTTACACTTAA+CGG | + | Chr3:101992761-101992780 | None:intergenic | 25.0% |
| ! | ATGAAAATTCTTGATATTGG+TGG | - | Chr3:101993249-101993268 | MsG0380017898.01.T01:CDS | 25.0% |
| ! | ATTTAGCGTTCTTGATTTAA+GGG | - | Chr3:101992675-101992694 | MsG0380017898.01.T01:CDS | 25.0% |
| ! | GATGCAATAAAAACACATTT+CGG | - | Chr3:101993321-101993340 | MsG0380017898.01.T01:CDS | 25.0% |
| ! | GGATAAAACAGATTCAATTT+CGG | + | Chr3:101992839-101992858 | None:intergenic | 25.0% |
| ! | TAATTGACCTTATGAACAAA+TGG | - | Chr3:101992701-101992720 | MsG0380017898.01.T01:CDS | 25.0% |
| ! | TGCCTATAAGTGAAATATTA+GGG | + | Chr3:101992774-101992793 | None:intergenic | 25.0% |
| AAAATTCTTGATATTGGTGG+TGG | - | Chr3:101993252-101993271 | MsG0380017898.01.T01:CDS | 30.0% | |
| AACTTCTTTGGCCAATAATA+TGG | + | Chr3:101993196-101993215 | None:intergenic | 30.0% | |
| AAGTCGAAAAGATCAAAAGA+TGG | - | Chr3:101992971-101992990 | MsG0380017898.01.T01:CDS | 30.0% | |
| AATGTAAATATGCTTAGCTG+TGG | + | Chr3:101993739-101993758 | None:intergenic | 30.0% | |
| ACGTTAGCAACAAAAGTTAT+TGG | - | Chr3:101993405-101993424 | MsG0380017898.01.T01:CDS | 30.0% | |
| CATTTAGCGTTCTTGATTTA+AGG | - | Chr3:101992674-101992693 | MsG0380017898.01.T01:CDS | 30.0% | |
| CGTTAGCAACAAAAGTTATT+GGG | - | Chr3:101993406-101993425 | MsG0380017898.01.T01:CDS | 30.0% | |
| CTTAAATCAAGAACGCTAAA+TGG | + | Chr3:101992676-101992695 | None:intergenic | 30.0% | |
| GTGCCTATAAGTGAAATATT+AGG | + | Chr3:101992775-101992794 | None:intergenic | 30.0% | |
| TCCTAAAACTTATGCTTCAA+CGG | - | Chr3:101993572-101993591 | MsG0380017898.01.T01:CDS | 30.0% | |
| TTACACTTAACGGCATAAAA+AGG | + | Chr3:101992751-101992770 | None:intergenic | 30.0% | |
| TTGTGATCCATTTGTTCATA+AGG | + | Chr3:101992711-101992730 | None:intergenic | 30.0% | |
| ! | AATTTGGGATTGAGATTCTT+CGG | + | Chr3:101992524-101992543 | None:intergenic | 30.0% |
| ! | AGCTGTTTCAAAAACTTCTT+TGG | + | Chr3:101993208-101993227 | None:intergenic | 30.0% |
| ! | ATTGAATCTGTTTTATCCCT+TGG | - | Chr3:101992841-101992860 | MsG0380017898.01.T01:CDS | 30.0% |
| !! | TGGATTGATGATGGAATTTA+CGG | - | Chr3:101993459-101993478 | MsG0380017898.01.T01:CDS | 30.0% |
| !!! | AGCTGGTTTGGTTTTAATAA+TGG | + | Chr3:101992641-101992660 | None:intergenic | 30.0% |
| AAACAGATTCAATTTCGGCA+CGG | + | Chr3:101992834-101992853 | None:intergenic | 35.0% | |
| AACACATTTCGGAAATGATG+AGG | - | Chr3:101993332-101993351 | MsG0380017898.01.T01:CDS | 35.0% | |
| ATGGATCACAAAACTTCCTA+CGG | - | Chr3:101992720-101992739 | MsG0380017898.01.T01:CDS | 35.0% | |
| ATGTGTGACTCTGATTTACA+CGG | + | Chr3:101992898-101992917 | None:intergenic | 35.0% | |
| GTATCAACTGAATCACAAGT+GGG | + | Chr3:101993606-101993625 | None:intergenic | 35.0% | |
| GTTGTATATGCACCCATATT+TGG | + | Chr3:101993684-101993703 | None:intergenic | 35.0% | |
| ! | ACCGTTGAAGCATAAGTTTT+AGG | + | Chr3:101993576-101993595 | None:intergenic | 35.0% |
| ! | ACTTATGCTTCAACGGTTTT+TGG | - | Chr3:101993579-101993598 | MsG0380017898.01.T01:CDS | 35.0% |
| ! | CACTTGTGATTCAGTTGATA+CGG | - | Chr3:101993605-101993624 | MsG0380017898.01.T01:CDS | 35.0% |
| ! | TGGCTTGTGTTTCCAAATAT+GGG | - | Chr3:101993669-101993688 | MsG0380017898.01.T01:CDS | 35.0% |
| ! | TTTGAAACAGCTTCACAACT+TGG | - | Chr3:101993216-101993235 | MsG0380017898.01.T01:CDS | 35.0% |
| !! | AGAGAGTATTGGATTGATGA+TGG | - | Chr3:101993450-101993469 | MsG0380017898.01.T01:CDS | 35.0% |
| !! | CTTCTGGAACCAATTTCAAT+GGG | - | Chr3:101993706-101993725 | MsG0380017898.01.T01:CDS | 35.0% |
| !! | GATGAGGATCTTGTTGTTAT+TGG | - | Chr3:101993348-101993367 | MsG0380017898.01.T01:CDS | 35.0% |
| !! | TCTTCTGGAACCAATTTCAA+TGG | - | Chr3:101993705-101993724 | MsG0380017898.01.T01:CDS | 35.0% |
| !! | TTGGCTTGTGTTTCCAAATA+TGG | - | Chr3:101993668-101993687 | MsG0380017898.01.T01:CDS | 35.0% |
| AACATTTACGCCAACACTTG+CGG | + | Chr3:101992929-101992948 | None:intergenic | 40.0% | |
| CAAAACCTGAGAATGTTGCA+TGG | - | Chr3:101993544-101993563 | MsG0380017898.01.T01:CDS | 40.0% | |
| CGTATCAACTGAATCACAAG+TGG | + | Chr3:101993607-101993626 | None:intergenic | 40.0% | |
| GAATGCAGCTTCATCAAATC+TGG | + | Chr3:101993292-101993311 | None:intergenic | 40.0% | |
| GATTCTTCGGTTACTAGTGT+AGG | + | Chr3:101992511-101992530 | None:intergenic | 40.0% | |
| GCATAAAAAGGCTTAACCGT+AGG | + | Chr3:101992739-101992758 | None:intergenic | 40.0% | |
| GGAGCTAAACCCATTGAAAT+TGG | + | Chr3:101993718-101993737 | None:intergenic | 40.0% | |
| GGTGCATATACAACTTCTTC+TGG | - | Chr3:101993690-101993709 | MsG0380017898.01.T01:CDS | 40.0% | |
| GTATGAGTTACCTGAACTTG+AGG | - | Chr3:101993638-101993657 | MsG0380017898.01.T01:CDS | 40.0% | |
| GTTACAACTTTCGACTGTGT+TGG | - | Chr3:101992946-101992965 | MsG0380017898.01.T01:CDS | 40.0% | |
| GTTGGCGTAAATGATTCTGT+CGG | + | Chr3:101992875-101992894 | None:intergenic | 40.0% | |
| TGTCTTCCTCCGGTTTAATA+CGG | + | Chr3:101993020-101993039 | None:intergenic | 40.0% | |
| TGTGTGACTCTGATTTACAC+GGG | + | Chr3:101992897-101992916 | None:intergenic | 40.0% | |
| TGTTCAACTCCAACTCTTCT+CGG | - | Chr3:101993762-101993781 | MsG0380017898.01.T01:CDS | 40.0% | |
| TTACCACGGAGCCATATTAT+TGG | - | Chr3:101993182-101993201 | MsG0380017898.01.T01:CDS | 40.0% | |
| TTGGCGTAAATGATTCTGTC+GGG | + | Chr3:101992874-101992893 | None:intergenic | 40.0% | |
| ! | AAATGCATGCCACTTAGGTT+TGG | - | Chr3:101993519-101993538 | MsG0380017898.01.T01:CDS | 40.0% |
| ! | ACCAAAAATGGGCTTCAAGT+TGG | + | Chr3:101992548-101992567 | None:intergenic | 40.0% |
| ! | ACCAACTTGAAGCCCATTTT+TGG | - | Chr3:101992544-101992563 | MsG0380017898.01.T01:CDS | 40.0% |
| ! | ACTCCTGAAGCACCAAAAAT+GGG | + | Chr3:101992559-101992578 | None:intergenic | 40.0% |
| ! | ATCTGAGATAGTAACCTCTG+AGG | + | Chr3:101992608-101992627 | None:intergenic | 40.0% |
| ! | GAGGTGAAATGAGAGAGTAT+TGG | - | Chr3:101993439-101993458 | MsG0380017898.01.T01:CDS | 40.0% |
| ! | TTGTCAAATGCATGCCACTT+AGG | - | Chr3:101993514-101993533 | MsG0380017898.01.T01:CDS | 40.0% |
| !! | AATGGTGAGTCATTTTCAGC+TGG | + | Chr3:101992658-101992677 | None:intergenic | 40.0% |
| !! | CTTGTTGTTATTGGTGAACC+TGG | - | Chr3:101993357-101993376 | MsG0380017898.01.T01:CDS | 40.0% |
| !! | GGGCTTCAAGTTGGTAATTT+GGG | + | Chr3:101992539-101992558 | None:intergenic | 40.0% |
| !! | TGAGTCATTTTCAGCTGGTT+TGG | + | Chr3:101992653-101992672 | None:intergenic | 40.0% |
| !! | TGGGCTTCAAGTTGGTAATT+TGG | + | Chr3:101992540-101992559 | None:intergenic | 40.0% |
| !!! | GGTTTTGAACCAAACCTAAG+TGG | + | Chr3:101993531-101993550 | None:intergenic | 40.0% |
| ATTAAACCGGAGGAAGACAG+TGG | - | Chr3:101993021-101993040 | MsG0380017898.01.T01:CDS | 45.0% | |
| ATTAAGTACGCCGCAAGTGT+TGG | - | Chr3:101992916-101992935 | MsG0380017898.01.T01:CDS | 45.0% | |
| CAATCGTTCACCTCAAGTTC+AGG | + | Chr3:101993651-101993670 | None:intergenic | 45.0% | |
| CTGAACTTGAGGTGAACGAT+TGG | - | Chr3:101993649-101993668 | MsG0380017898.01.T01:CDS | 45.0% | |
| GTAGCTCGGGAACTTCATTT+AGG | + | Chr3:101993083-101993102 | None:intergenic | 45.0% | |
| GTGGCTGGACTTAAAGTAAC+CGG | - | Chr3:101993117-101993136 | MsG0380017898.01.T01:CDS | 45.0% | |
| GTTATTGGGAAACGTGTGAG+AGG | - | Chr3:101993420-101993439 | MsG0380017898.01.T01:CDS | 45.0% | |
| GTTCAACTCCAACTCTTCTC+GGG | - | Chr3:101993763-101993782 | MsG0380017898.01.T01:CDS | 45.0% | |
| GTTTCTTTCCACATAGGCAG+TGG | - | Chr3:101993141-101993160 | MsG0380017898.01.T01:CDS | 45.0% | |
| TCGTGTCTGGGTCTCAAATA+TGG | - | Chr3:101993051-101993070 | MsG0380017898.01.T01:CDS | 45.0% | |
| TCTCTCCATGCAACATTCTC+AGG | + | Chr3:101993552-101993571 | None:intergenic | 45.0% | |
| TGACTCTGATTTACACGGGT+TGG | + | Chr3:101992893-101992912 | None:intergenic | 45.0% | |
| ! | ATAGGCACACTAGCTTCACT+CGG | - | Chr3:101992787-101992806 | MsG0380017898.01.T01:CDS | 45.0% |
| ! | ATTTGAGACCCAGACACGAA+CGG | + | Chr3:101993050-101993069 | None:intergenic | 45.0% |
| ! | CACTCCTGAAGCACCAAAAA+TGG | + | Chr3:101992560-101992579 | None:intergenic | 45.0% |
| ! | TGAACCTGGTCGTTACTTTG+CGG | - | Chr3:101993371-101993390 | MsG0380017898.01.T01:CDS | 45.0% |
| ! | TGGTCGTTACTTTGCGGAAA+CGG | - | Chr3:101993377-101993396 | MsG0380017898.01.T01:CDS | 45.0% |
| !! | ATCAGCAGCTTTGAGTAGCT+CGG | + | Chr3:101993097-101993116 | None:intergenic | 45.0% |
| !! | ATTGGTGGTGGATTCACTTC+TGG | - | Chr3:101993264-101993283 | MsG0380017898.01.T01:CDS | 45.0% |
| !! | TTTGGTGCTTCAGGAGTGAA+AGG | - | Chr3:101992562-101992581 | MsG0380017898.01.T01:CDS | 45.0% |
| !!! | AAGCCCATTTTTGGTGCTTC+AGG | - | Chr3:101992553-101992572 | MsG0380017898.01.T01:CDS | 45.0% |
| ACCGGCGTTTCTTTCCACAT+AGG | - | Chr3:101993135-101993154 | MsG0380017898.01.T01:CDS | 50.0% | |
| AGATTCAATTTCGGCACGGC+TGG | + | Chr3:101992830-101992849 | None:intergenic | 50.0% | |
| AGCGACAACTTTGTCCTCAG+AGG | - | Chr3:101992591-101992610 | MsG0380017898.01.T01:CDS | 50.0% | |
| CTTCCTCCGGTTTAATACGG+AGG | + | Chr3:101993017-101993036 | None:intergenic | 50.0% | |
| GCCTATGTGGAAAGAAACGC+CGG | + | Chr3:101993139-101993158 | None:intergenic | 50.0% | |
| GCTACTCAAAGCTGCTGATG+TGG | - | Chr3:101993098-101993117 | MsG0380017898.01.T01:CDS | 50.0% | |
| GCTCCTCCTCCGTATTAAAC+CGG | - | Chr3:101993008-101993027 | MsG0380017898.01.T01:CDS | 50.0% | |
| GTTTCCGCAAAGTAACGACC+AGG | + | Chr3:101993378-101993397 | None:intergenic | 50.0% | |
| TCTTTCCACATAGGCAGTGG+AGG | - | Chr3:101993144-101993163 | MsG0380017898.01.T01:CDS | 50.0% | |
| TGGCCAATAATATGGCTCCG+TGG | + | Chr3:101993188-101993207 | None:intergenic | 50.0% | |
| ! | TCCGTGGTAAGCTTTTGCGT+CGG | + | Chr3:101993172-101993191 | None:intergenic | 50.0% |
| ! | TTTGAGACCCAGACACGAAC+GGG | + | Chr3:101993049-101993068 | None:intergenic | 50.0% |
| !! | TCAGCAGCTTTGAGTAGCTC+GGG | + | Chr3:101993096-101993115 | None:intergenic | 50.0% |
| CCTCCGGTTTAATACGGAGG+AGG | + | Chr3:101993014-101993033 | None:intergenic | 55.0% | |
| CCTCCTCCGTATTAAACCGG+AGG | - | Chr3:101993011-101993030 | MsG0380017898.01.T01:CDS | 55.0% | |
| CTCAAAGCTGCTGATGTGGC+TGG | - | Chr3:101993102-101993121 | MsG0380017898.01.T01:CDS | 55.0% | |
| GCCGACGCAAAAGCTTACCA+CGG | - | Chr3:101993168-101993187 | MsG0380017898.01.T01:CDS | 55.0% | |
| TCTGTCGGGTGAAACGCCAA+GGG | + | Chr3:101992860-101992879 | None:intergenic | 55.0% | |
| TTCTGTCGGGTGAAACGCCA+AGG | + | Chr3:101992861-101992880 | None:intergenic | 55.0% | |
| ! | GGAGGAGGAGCTCGCATTTT+GGG | + | Chr3:101992999-101993018 | None:intergenic | 55.0% |
| ACGGCTGGCGCAGTCGAAAT+TGG | + | Chr3:101992815-101992834 | None:intergenic | 60.0% | |
| AGTGGAGCCCGTTCGTGTCT+GGG | - | Chr3:101993039-101993058 | MsG0380017898.01.T01:CDS | 60.0% | |
| ! | CGGAGGAGGAGCTCGCATTT+TGG | + | Chr3:101993000-101993019 | None:intergenic | 60.0% |
| CAGTGGAGCCCGTTCGTGTC+TGG | - | Chr3:101993038-101993057 | MsG0380017898.01.T01:CDS | 65.0% | |
| CGGCTCCTCCACTGCCTATG+TGG | + | Chr3:101993152-101993171 | None:intergenic | 65.0% | |
| CGGGCTCCACTGTCTTCCTC+CGG | + | Chr3:101993030-101993049 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 101992505 | 101993791 | 101992505 | ID=MsG0380017898.01;Name=MsG0380017898.01 |
| Chr3 | mRNA | 101992505 | 101993791 | 101992505 | ID=MsG0380017898.01.T01;Parent=MsG0380017898.01;Name=MsG0380017898.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|428 |
| Chr3 | exon | 101992505 | 101993791 | 101992505 | ID=MsG0380017898.01.T01:exon:18221;Parent=MsG0380017898.01.T01 |
| Chr3 | CDS | 101992505 | 101993791 | 101992505 | ID=MsG0380017898.01.T01:cds;Parent=MsG0380017898.01.T01 |
| Gene Sequence |
| Protein sequence |
