AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003568.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003568.01.T01 MTR_1g064260 90.868 219 4 1 1 203 1 219 2.92e-144 400
MsG0180003568.01.T01 MTR_7g101290 85.648 216 15 1 4 203 5 220 4.62e-137 382
MsG0180003568.01.T01 MTR_0041s0100 85.648 216 15 1 4 203 5 220 4.62e-137 382
MsG0180003568.01.T01 MTR_2g094790 77.778 207 30 1 1 191 1 207 8.13e-115 326
MsG0180003568.01.T01 MTR_4g058740 80.829 193 21 1 12 188 12 204 2.62e-111 317
MsG0180003568.01.T01 MTR_3g113710 69.524 210 48 1 10 203 12 221 5.34e-106 304
MsG0180003568.01.T01 MTR_1g064260 92.949 156 11 0 48 203 8 163 4.48e-104 297
MsG0180003568.01.T01 MTR_1g057020 63.636 209 58 2 10 202 6 212 6.54e-94 273
MsG0180003568.01.T01 MTR_3g113710 70.950 179 36 1 10 172 12 190 1.60e-90 265
MsG0180003568.01.T01 MTR_1g015830 61.881 202 61 1 11 196 7 208 1.46e-88 259
MsG0180003568.01.T01 MTR_3g113710 76.224 143 34 0 61 203 13 155 4.19e-79 233
MsG0180003568.01.T01 MTR_1g015830 63.758 149 54 0 48 196 8 156 2.22e-65 199
MsG0180003568.01.T01 MTR_1g024145 60.194 103 25 1 28 114 150 252 2.94e-33 120
MsG0180003568.01.T01 MTR_1g024850 72.917 48 13 0 10 57 14 61 3.80e-20 81.6
MsG0180003568.01.T02 MTR_1g064260 93.023 86 6 0 1 86 78 163 2.72e-55 168
MsG0180003568.01.T02 MTR_1g064260 93.023 86 6 0 1 86 134 219 1.22e-54 168
MsG0180003568.01.T02 MTR_7g101290 88.372 86 10 0 1 86 135 220 6.66e-53 164
MsG0180003568.01.T02 MTR_0041s0100 88.372 86 10 0 1 86 135 220 6.66e-53 164
MsG0180003568.01.T02 MTR_3g113710 63.953 86 31 0 1 86 70 155 5.33e-36 119
MsG0180003568.01.T02 MTR_3g113710 63.953 86 31 0 1 86 136 221 2.67e-35 119
MsG0180003568.01.T02 MTR_2g094790 74.324 74 19 0 1 74 134 207 2.23e-34 117
MsG0180003568.01.T02 MTR_4g058740 76.056 71 17 0 1 71 134 204 1.31e-33 115
MsG0180003568.01.T02 MTR_1g015830 58.228 79 33 0 1 79 78 156 3.79e-27 97.1
MsG0180003568.01.T02 MTR_1g015830 58.228 79 33 0 1 79 130 208 1.42e-26 97.1
MsG0180003568.01.T02 MTR_1g057020 57.647 85 34 1 1 85 130 212 3.42e-26 95.9
MsG0180003568.01.T02 MTR_3g113710 65.455 55 19 0 1 55 136 190 3.74e-20 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003568.01.T01 AT2G37210 77.674 215 30 3 1 198 1 214 9.33e-118 333
MsG0180003568.01.T01 AT3G53450 77.404 208 30 2 1 191 1 208 4.82e-115 327
MsG0180003568.01.T01 AT2G28305 81.818 198 20 1 10 191 5 202 9.07e-115 326
MsG0180003568.01.T01 AT2G37210 69.456 239 31 4 1 198 1 238 1.58e-111 318
MsG0180003568.01.T01 AT2G28305 82.123 179 16 1 10 172 5 183 3.21e-103 296
MsG0180003568.01.T01 AT5G06300 70.297 202 44 1 10 195 6 207 2.84e-101 292
MsG0180003568.01.T01 AT5G03270 73.118 186 34 1 10 179 16 201 5.80e-94 273
MsG0180003568.01.T01 AT4G35190 65.025 203 55 1 6 192 2 204 1.34e-93 273
MsG0180003568.01.T01 AT5G03270 73.481 181 32 1 10 174 16 196 9.57e-91 264
MsG0180003568.01.T01 AT4G35190 66.321 193 47 2 6 182 2 192 4.17e-88 258
MsG0180003568.01.T01 AT2G35990 61.340 194 59 1 10 187 6 199 7.61e-88 258
MsG0180003568.01.T01 AT5G11950 58.962 212 68 2 1 196 1 209 1.75e-87 257
MsG0180003568.01.T01 AT5G11950 58.962 212 68 2 1 196 1 209 1.75e-87 257
MsG0180003568.01.T01 AT5G11950 58.962 212 68 2 1 196 1 209 1.75e-87 257
MsG0180003568.01.T01 AT2G28305 80.303 132 10 1 10 125 5 136 2.04e-70 211
MsG0180003568.01.T01 AT5G11950 61.783 157 57 1 40 196 3 156 7.31e-65 197
MsG0180003568.01.T01 AT2G35990 64.286 140 50 0 48 187 8 147 3.27e-63 193
MsG0180003568.01.T01 AT2G35990 64.286 140 50 0 48 187 8 147 3.27e-63 193
MsG0180003568.01.T01 AT5G26140 51.656 151 51 1 68 196 6 156 4.88e-47 152
MsG0180003568.01.T01 AT5G26140 50.365 137 46 1 82 196 1 137 1.10e-39 133
MsG0180003568.01.T01 AT5G26140 53.774 106 49 0 91 196 17 122 2.40e-35 121
MsG0180003568.01.T02 AT2G37210 72.840 81 21 1 1 81 135 214 1.84e-37 125
MsG0180003568.01.T02 AT2G28305 72.093 86 23 1 1 86 129 213 2.01e-37 124
MsG0180003568.01.T02 AT2G37210 72.840 81 21 1 1 81 159 238 2.77e-37 125
MsG0180003568.01.T02 AT3G53450 74.324 74 19 0 1 74 135 208 4.61e-36 121
MsG0180003568.01.T02 AT5G11950 56.962 79 34 0 1 79 78 156 2.78e-28 100
MsG0180003568.01.T02 AT5G06300 60.256 78 31 0 1 78 130 207 1.12e-27 100
MsG0180003568.01.T02 AT5G11950 56.962 79 34 0 1 79 131 209 1.28e-27 99.8
MsG0180003568.01.T02 AT5G11950 56.962 79 34 0 1 79 131 209 1.28e-27 99.8
MsG0180003568.01.T02 AT5G11950 56.962 79 34 0 1 79 131 209 1.28e-27 99.8
MsG0180003568.01.T02 AT4G35190 58.667 75 31 0 1 75 130 204 2.91e-27 99.4
MsG0180003568.01.T02 AT5G03270 69.355 62 19 0 1 62 140 201 2.03e-25 94.0
MsG0180003568.01.T02 AT2G28305 80.000 55 11 0 1 55 129 183 2.17e-25 93.6
MsG0180003568.01.T02 AT2G35990 57.143 70 30 0 1 70 78 147 1.71e-24 90.5
MsG0180003568.01.T02 AT2G35990 57.143 70 30 0 1 70 78 147 1.71e-24 90.5
MsG0180003568.01.T02 AT5G26140 51.899 79 38 0 1 79 44 122 1.94e-24 89.4
MsG0180003568.01.T02 AT5G26140 51.899 79 38 0 1 79 59 137 2.16e-24 89.7
MsG0180003568.01.T02 AT5G26140 51.899 79 38 0 1 79 78 156 3.27e-24 89.7
MsG0180003568.01.T02 AT2G35990 57.143 70 30 0 1 70 130 199 7.57e-24 90.1
MsG0180003568.01.T02 AT5G03270 70.175 57 17 0 1 57 140 196 3.59e-22 85.5
MsG0180003568.01.T02 AT4G35190 61.538 65 23 1 1 65 130 192 1.47e-21 84.0

Find 44 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAATATTGATCTAGTTTA+TGG 0.173128 1:+64322490 MsG0180003568.01.T01:CDS
AAATTACATGCAGGTGGTTA+TGG 0.290968 1:+64324267 MsG0180003568.01.T01:intron
CAAGATGCTGCCATTGAACT+TGG 0.349737 1:+64322031 MsG0180003568.01.T01:CDS
ACAATTATGTGGCGCGCATT+TGG 0.351381 1:-64324532 None:intergenic
AAAAGAGTTAGTGAAGAAAC+TGG 0.405307 1:+64324570 MsG0180003568.01.T01:CDS
CATTGAACTTGGAAATGAAT+TGG 0.407304 1:+64322042 MsG0180003568.01.T01:CDS
AATTACATGCAGGTGGTTAT+GGG 0.415602 1:+64324268 MsG0180003568.01.T01:intron
AGGAGGAAGCATTGGTCTAA+TGG 0.437267 1:+64322513 MsG0180003568.01.T01:CDS
GTCATAACCTGGGCTCAACT+TGG 0.447889 1:+64324312 MsG0180003568.01.T01:CDS
CTCTCTAGGCATGAGTGTCT+TGG 0.461900 1:-64322720 None:intergenic
CAATTCATTTCCAAGTTCAA+TGG 0.462751 1:-64322041 None:intergenic
TTGCAAGAAACAAATACAAA+TGG 0.467273 1:+64321925 MsG0180003568.01.T01:exon
GTGGGACTAGTAAATGTTGA+TGG 0.489170 1:+64324451 MsG0180003568.01.T01:CDS
ATTGATCTAGTTTATGGAGG+AGG 0.513882 1:+64322496 MsG0180003568.01.T01:CDS
CAAATACAAATGGAGACAAA+TGG 0.514050 1:+64321935 MsG0180003568.01.T01:exon
TAGCTCAGGCTAACCTGGTA+AGG 0.520557 1:-64323260 None:intergenic
TGACTTCAAGAAGCTCCTCT+AGG 0.523303 1:-64324293 None:intergenic
CATCGACAAAGCTGTGGAAG+AGG 0.532010 1:+64324498 MsG0180003568.01.T01:CDS
AGCTTCTTGAAGTCATAACC+TGG 0.532925 1:+64324301 MsG0180003568.01.T01:CDS
TGAAATGAAATGCTATTGCT+TGG 0.533951 1:-64321730 None:intergenic
GTTTATGGAGGAGGAAGCAT+TGG 0.534440 1:+64322505 MsG0180003568.01.T01:CDS
TTTGCAGCTAACTGGTGAAA+CGG 0.542234 1:+64323162 MsG0180003568.01.T01:intron
CTGTAGCAGATATGCATCAA+AGG 0.549564 1:+64323200 MsG0180003568.01.T01:CDS
GACTTCAAGAAGCTCCTCTA+GGG 0.553887 1:-64324292 None:intergenic
TCATAACCTGGGCTCAACTT+GGG 0.556522 1:+64324313 MsG0180003568.01.T01:CDS
TAAGAGTGTCAAAGTTCAAG+AGG 0.560240 1:+64321963 MsG0180003568.01.T01:CDS
GACATTCCACAACTCGCAGG+TGG 0.562576 1:+64324432 MsG0180003568.01.T01:intron
GCATCAAAGGAAGGCAGAGA+TGG 0.563589 1:+64323213 MsG0180003568.01.T01:CDS
CTAGTCCCACCTGCGAGTTG+TGG 0.568338 1:-64324438 None:intergenic
TTCATTCATCGACAAAGCTG+TGG 0.574795 1:+64324492 MsG0180003568.01.T01:CDS
AATATTGATCTAGTTTATGG+AGG 0.578824 1:+64322493 MsG0180003568.01.T01:CDS
ATGAATCCCAAGTTGAGCCC+AGG 0.585691 1:-64324319 None:intergenic
ATCGACAAAGCTGTGGAAGA+GGG 0.594245 1:+64324499 MsG0180003568.01.T01:CDS
TTTGACATTCCACAACTCGC+AGG 0.597104 1:+64324429 MsG0180003568.01.T01:intron
CAGCTAACTGGTGAAACGGT+GGG 0.599390 1:+64323166 MsG0180003568.01.T01:intron
GCAGCTAACTGGTGAAACGG+TGG 0.613685 1:+64323165 MsG0180003568.01.T01:intron
CAAGACACTCATGCCTAGAG+AGG 0.615363 1:+64322721 MsG0180003568.01.T01:CDS
AAGAGTGTCAAAGTTCAAGA+GGG 0.616352 1:+64321964 MsG0180003568.01.T01:CDS
AGCAGATATGCATCAAAGGA+AGG 0.629113 1:+64323204 MsG0180003568.01.T01:CDS
ACATTCCACAACTCGCAGGT+GGG 0.652643 1:+64324433 MsG0180003568.01.T01:intron
GCTTCTTGAAGTCATAACCT+GGG 0.658696 1:+64324302 MsG0180003568.01.T01:CDS
TAGGTGCTGATACAATTATG+TGG 0.681813 1:-64324543 None:intergenic
AGAGTTAGTGAAGAAACTGG+AGG 0.710150 1:+64324573 MsG0180003568.01.T01:CDS
AGGTGGTTATGGGACCCTAG+AGG 0.728214 1:+64324278 MsG0180003568.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATATGAAAAAAACTTT+AGG - Chr1:64322353-64322372 None:intergenic 10.0%
!! TAAAAGTAATAATAAAGAAA+TGG + Chr1:64322976-64322995 MsG0180003568.01.T01:intron 10.0%
!! TGAATTAGTTAATAATATTT+AGG - Chr1:64324382-64324401 None:intergenic 10.0%
!!! AAAATCTTATTTATTTTTTG+TGG + Chr1:64322127-64322146 MsG0180003568.01.T01:intron 10.0%
!! ATGAAAAAAGTGAATAATAA+AGG - Chr1:64322828-64322847 None:intergenic 15.0%
!! ATTTAGAAATAGATTACTTA+AGG - Chr1:64325038-64325057 None:intergenic 15.0%
!! TAACAAACTTAATTACTAAA+AGG + Chr1:64323417-64323436 MsG0180003568.01.T01:intron 15.0%
!! TTTAGAAATAGATTACTTAA+GGG - Chr1:64325037-64325056 None:intergenic 15.0%
!!! TATTAAGTTTGAAATGAAAA+TGG - Chr1:64322423-64322442 None:intergenic 15.0%
!!! TGTTAAAGTTATTTTCTTTT+GGG + Chr1:64322929-64322948 MsG0180003568.01.T01:intron 15.0%
!!! TTGTTAAAGTTATTTTCTTT+TGG + Chr1:64322928-64322947 MsG0180003568.01.T01:intron 15.0%
!!! TTTTAGTAATTAAGTTTGTT+AGG - Chr1:64323418-64323437 None:intergenic 15.0%
!! AAATTTCATTTGTAAAGTAC+TGG + Chr1:64324893-64324912 MsG0180003568.01.T01:three_prime_UTR 20.0%
!! ACAAACCATTAAAATTGATT+GGG - Chr1:64322460-64322479 None:intergenic 20.0%
!! AGAATTTAATGCAAAAACAT+AGG - Chr1:64324233-64324252 None:intergenic 20.0%
!! TCATAAAAATGAAAACTTTG+AGG - Chr1:64322598-64322617 None:intergenic 20.0%
!! TTAACTTAAAATACAGAACA+GGG - Chr1:64322883-64322902 None:intergenic 20.0%
!! TTAGAAATAGATTACTTAAG+GGG - Chr1:64325036-64325055 None:intergenic 20.0%
!!! AAATTTTAGCCAAATATGTT+AGG - Chr1:64323660-64323679 None:intergenic 20.0%
!!! AATTTCATTTGTAAAGTACT+GGG + Chr1:64324894-64324913 MsG0180003568.01.T01:three_prime_UTR 20.0%
!!! AATTTTAGCCAAATATGTTA+GGG - Chr1:64323659-64323678 None:intergenic 20.0%
!!! AGAAATATTGATCTAGTTTA+TGG + Chr1:64322490-64322509 MsG0180003568.01.T01:CDS 20.0%
!!! AGTAATTTCACATTTTTTTC+AGG - Chr1:64324925-64324944 None:intergenic 20.0%
!!! CTTATTTATTTTTTGTGGAT+GGG + Chr1:64322132-64322151 MsG0180003568.01.T01:intron 20.0%
!!! GATTTTTTTTTCTCAAAAGA+AGG - Chr1:64322113-64322132 None:intergenic 20.0%
!!! TCTTATTTATTTTTTGTGGA+TGG + Chr1:64322131-64322150 MsG0180003568.01.T01:intron 20.0%
!!! TTATTTATTTTTTGTGGATG+GGG + Chr1:64322133-64322152 MsG0180003568.01.T01:intron 20.0%
!!! TTTTCAAAGACTTTTATTTC+TGG + Chr1:64323876-64323895 MsG0180003568.01.T01:intron 20.0%
! AAAAAAAATGATCTGACCAT+TGG + Chr1:64324104-64324123 MsG0180003568.01.T01:intron 25.0%
! AATCATTAATATTGTTGCCA+AGG + Chr1:64323452-64323471 MsG0180003568.01.T01:intron 25.0%
! AATGTCAAATCTAGTTAAAC+TGG - Chr1:64324418-64324437 None:intergenic 25.0%
! AATTCCTTGTATATTTCAGT+TGG + Chr1:64322198-64322217 MsG0180003568.01.T01:intron 25.0%
! ACAGATTCCTACAATAATTA+TGG - Chr1:64324164-64324183 None:intergenic 25.0%
! ATTATTGTAGGAATCTGTTT+CGG + Chr1:64324166-64324185 MsG0180003568.01.T01:intron 25.0%
! ATTCCTTGTATATTTCAGTT+GGG + Chr1:64322199-64322218 MsG0180003568.01.T01:intron 25.0%
! CACAAACCATTAAAATTGAT+TGG - Chr1:64322461-64322480 None:intergenic 25.0%
! CTTAACTTAAAATACAGAAC+AGG - Chr1:64322884-64322903 None:intergenic 25.0%
! GGAAAAAAAAACAGTATGTA+AGG - Chr1:64322092-64322111 None:intergenic 25.0%
! TAGTCAAATAAATATGACAC+TGG + Chr1:64323547-64323566 MsG0180003568.01.T01:intron 25.0%
! TATTATGTTTGATTCATCAC+AGG + Chr1:64324667-64324686 MsG0180003568.01.T01:intron 25.0%
! TGGCAACAATATTAATGATT+AGG - Chr1:64323452-64323471 None:intergenic 25.0%
! TTACAAATGAAATTTCTTCC+AGG - Chr1:64324888-64324907 None:intergenic 25.0%
! TTGCAAGAAACAAATACAAA+TGG + Chr1:64321925-64321944 MsG0180003568.01.T01:exon 25.0%
!! AATATTGATCTAGTTTATGG+AGG + Chr1:64322493-64322512 MsG0180003568.01.T01:CDS 25.0%
!! CTTATCCCAATCAATTTTAA+TGG + Chr1:64322452-64322471 MsG0180003568.01.T01:intron 25.0%
!! GAAATCAGTTTTCAAAAGAA+GGG - Chr1:64324953-64324972 None:intergenic 25.0%
!! TATTTAGGTTCCTTAACAAA+TGG - Chr1:64324367-64324386 None:intergenic 25.0%
!!! AAAGTTATTTTCTTTTGGGT+AGG + Chr1:64322933-64322952 MsG0180003568.01.T01:intron 25.0%
!!! TCAATTTTAATGGTTTGTGA+AGG + Chr1:64322462-64322481 MsG0180003568.01.T01:intron 25.0%
AAAAGAGTTAGTGAAGAAAC+TGG + Chr1:64324570-64324589 MsG0180003568.01.T01:CDS 30.0%
AAAATACAGAACAGGGAAAT+AGG - Chr1:64322876-64322895 None:intergenic 30.0%
AAATTAGTGACTGTTACAGA+AGG + Chr1:64323924-64323943 MsG0180003568.01.T01:intron 30.0%
AACTTATGCAAATTACATGC+AGG + Chr1:64324258-64324277 MsG0180003568.01.T01:intron 30.0%
AAGAATAGTACACCTTACTT+TGG + Chr1:64323283-64323302 MsG0180003568.01.T01:intron 30.0%
AAGCATTAAAGACTGAGATA+TGG - Chr1:64323385-64323404 None:intergenic 30.0%
ACAATATTAATGATTAGGCG+TGG - Chr1:64323447-64323466 None:intergenic 30.0%
ACAGTGTATCAAAATTACCT+TGG - Chr1:64323472-64323491 None:intergenic 30.0%
AGGAAAGTAGATGCAAAAAA+TGG - Chr1:64323728-64323747 None:intergenic 30.0%
ATCAAAACCATGAGTAAGTT+TGG - Chr1:64323771-64323790 None:intergenic 30.0%
CAAATACAAATGGAGACAAA+TGG + Chr1:64321935-64321954 MsG0180003568.01.T01:exon 30.0%
CAAATGGTACAAGTCAATTA+CGG - Chr1:64324351-64324370 None:intergenic 30.0%
CAATTCATTTCCAAGTTCAA+TGG - Chr1:64322044-64322063 None:intergenic 30.0%
CATTGAACTTGGAAATGAAT+TGG + Chr1:64322042-64322061 MsG0180003568.01.T01:CDS 30.0%
CTAGTACTTACAACTTAGAA+GGG - Chr1:64324975-64324994 None:intergenic 30.0%
CTTCTAAGTTGTAAGTACTA+GGG + Chr1:64324974-64324993 MsG0180003568.01.T01:three_prime_UTR 30.0%
GTTGTTCGATTTCAAATCAA+TGG - Chr1:64324062-64324081 None:intergenic 30.0%
TCAAGCACAATAGACTATTA+TGG - Chr1:64322629-64322648 None:intergenic 30.0%
TCTCTAATCATCATAGCATT+AGG - Chr1:64323596-64323615 None:intergenic 30.0%
TGAAATGAAATGCTATTGCT+TGG - Chr1:64321733-64321752 None:intergenic 30.0%
TTCTAAGTTGTAAGTACTAG+GGG + Chr1:64324975-64324994 MsG0180003568.01.T01:three_prime_UTR 30.0%
! GAGACTAGATAGAATTTTAG+AGG + Chr1:64324820-64324839 MsG0180003568.01.T01:three_prime_UTR 30.0%
! GATACTTTAAAAAGCTGAGA+AGG - Chr1:64322401-64322420 None:intergenic 30.0%
! GGAAATCAGTTTTCAAAAGA+AGG - Chr1:64324954-64324973 None:intergenic 30.0%
! TTCACTAACTCTTTTGATGT+AGG - Chr1:64324565-64324584 None:intergenic 30.0%
! TTTAGTTTACTTGATGTGCA+AGG + Chr1:64324792-64324811 MsG0180003568.01.T01:three_prime_UTR 30.0%
! TTTGGTGCATATGTAAATCT+CGG + Chr1:64324036-64324055 MsG0180003568.01.T01:intron 30.0%
! TTTTGTACCAAACTTACTCA+TGG + Chr1:64323761-64323780 MsG0180003568.01.T01:intron 30.0%
!! ACTAGATAGAATTTTAGAGG+TGG + Chr1:64324823-64324842 MsG0180003568.01.T01:three_prime_UTR 30.0%
!! TTGACTTGTACCATTTGTTA+AGG + Chr1:64324354-64324373 MsG0180003568.01.T01:intron 30.0%
!!! ACTCTTTTGTTTTACTTCAG+TGG + Chr1:64323006-64323025 MsG0180003568.01.T01:intron 30.0%
!!! CTCATGGTTTTGATATAGAA+AGG + Chr1:64323777-64323796 MsG0180003568.01.T01:intron 30.0%
!!! TTTTTTGCATCTACTTTCCT+TGG + Chr1:64323728-64323747 MsG0180003568.01.T01:intron 30.0%
AAAGACTGAGATATGGTCAA+TGG - Chr1:64323378-64323397 None:intergenic 35.0%
AAATTACATGCAGGTGGTTA+TGG + Chr1:64324267-64324286 MsG0180003568.01.T01:intron 35.0%
AATTACATGCAGGTGGTTAT+GGG + Chr1:64324268-64324287 MsG0180003568.01.T01:intron 35.0%
ACTTGTCATTCACTCTTTCT+TGG + Chr1:64323964-64323983 MsG0180003568.01.T01:intron 35.0%
AGGATCCAATGGATAAGAAA+TGG - Chr1:64323141-64323160 None:intergenic 35.0%
ATGCAAAAACATAGGAGAAC+AGG - Chr1:64324225-64324244 None:intergenic 35.0%
CACTATTTCATGACATGTAC+AGG - Chr1:64323852-64323871 None:intergenic 35.0%
CCAGGAAGAAAAACAGAATA+GGG - Chr1:64324870-64324889 None:intergenic 35.0%
CCTAGTACTTACAACTTAGA+AGG - Chr1:64324976-64324995 None:intergenic 35.0%
CCTTCTAAGTTGTAAGTACT+AGG + Chr1:64324973-64324992 MsG0180003568.01.T01:three_prime_UTR 35.0%
CTGTGTGCCATAATTATTGT+AGG + Chr1:64324154-64324173 MsG0180003568.01.T01:intron 35.0%
CTGTTCCATTTCTTATCCAT+TGG + Chr1:64323133-64323152 MsG0180003568.01.T01:intron 35.0%
CTTGTCATTCACTCTTTCTT+GGG + Chr1:64323965-64323984 MsG0180003568.01.T01:intron 35.0%
GACAAATAAACCAGTGTCAA+TGG - Chr1:64322674-64322693 None:intergenic 35.0%
TACAAAACGTCATGATTCCA+AGG - Chr1:64323748-64323767 None:intergenic 35.0%
TCCAGGAAGAAAAACAGAAT+AGG - Chr1:64324871-64324890 None:intergenic 35.0%
TCTAAGTTGTAAGTACTAGG+GGG + Chr1:64324976-64324995 MsG0180003568.01.T01:three_prime_UTR 35.0%
TCTGAAAAACTATGGTTCTC+TGG + Chr1:64323694-64323713 MsG0180003568.01.T01:intron 35.0%
TCTGTAATGCAACCAAAGTA+AGG - Chr1:64323298-64323317 None:intergenic 35.0%
TGACCCAACTGAAATATACA+AGG - Chr1:64322205-64322224 None:intergenic 35.0%
TTATGCAAATTACATGCAGG+TGG + Chr1:64324261-64324280 MsG0180003568.01.T01:intron 35.0%
! ATTGATCTAGTTTATGGAGG+AGG + Chr1:64322496-64322515 MsG0180003568.01.T01:CDS 35.0%
! CACTTAAGGTCATCTAAAAC+TGG - Chr1:64324200-64324219 None:intergenic 35.0%
! GCTTTGACTCTGAAAAACTA+TGG + Chr1:64323686-64323705 MsG0180003568.01.T01:intron 35.0%
!! AAGAGTGTCAAAGTTCAAGA+GGG + Chr1:64321964-64321983 MsG0180003568.01.T01:CDS 35.0%
!! TAAGAGTGTCAAAGTTCAAG+AGG + Chr1:64321963-64321982 MsG0180003568.01.T01:CDS 35.0%
!! TAGGTGCTGATACAATTATG+TGG - Chr1:64324546-64324565 None:intergenic 35.0%
!! TGATAACTACTTTTCTTGCC+AGG - Chr1:64322013-64322032 None:intergenic 35.0%
AAGATAGAGGGAAAGAGAGA+GGG + Chr1:64323997-64324016 MsG0180003568.01.T01:intron 40.0%
AGAGAGTGACTCTTCAAAAC+TGG + Chr1:64323611-64323630 MsG0180003568.01.T01:intron 40.0%
AGAGTTAGTGAAGAAACTGG+AGG + Chr1:64324573-64324592 MsG0180003568.01.T01:CDS 40.0%
AGCAGATATGCATCAAAGGA+AGG + Chr1:64323204-64323223 MsG0180003568.01.T01:CDS 40.0%
AGCTTCTTGAAGTCATAACC+TGG + Chr1:64324301-64324320 MsG0180003568.01.T01:CDS 40.0%
ATCTCTTTGTCCATTGACAC+TGG + Chr1:64322661-64322680 MsG0180003568.01.T01:intron 40.0%
CTGTAGCAGATATGCATCAA+AGG + Chr1:64323200-64323219 MsG0180003568.01.T01:CDS 40.0%
CTTGAGATGTCATACTCTTC+AGG - Chr1:64324761-64324780 None:intergenic 40.0%
GATGTCATACTCTTCAGGAT+AGG - Chr1:64324756-64324775 None:intergenic 40.0%
GATTATGTTCCTTGTCACGA+AGG + Chr1:64324689-64324708 MsG0180003568.01.T01:CDS 40.0%
GCTTCTTGAAGTCATAACCT+GGG + Chr1:64324302-64324321 MsG0180003568.01.T01:CDS 40.0%
GGTGATAAGGGGTCTTAATT+TGG + Chr1:64324018-64324037 MsG0180003568.01.T01:intron 40.0%
GTTTCACAAGCTGTTCATGA+TGG + Chr1:64322540-64322559 MsG0180003568.01.T01:intron 40.0%
TAGCTGCAAAAAGGATCCAA+TGG - Chr1:64323152-64323171 None:intergenic 40.0%
TAGGAGAACAGGAACACTTA+AGG - Chr1:64324214-64324233 None:intergenic 40.0%
TCTTTCCCTCTATCTTGCAA+GGG - Chr1:64323993-64324012 None:intergenic 40.0%
TTCACCAGTTAGCTGCAAAA+AGG - Chr1:64323161-64323180 None:intergenic 40.0%
TTCAGTGGCTTATAATGTGC+TGG + Chr1:64323021-64323040 MsG0180003568.01.T01:intron 40.0%
TTCATTCATCGACAAAGCTG+TGG + Chr1:64324492-64324511 MsG0180003568.01.T01:CDS 40.0%
TTTGCAGCTAACTGGTGAAA+CGG + Chr1:64323162-64323181 MsG0180003568.01.T01:intron 40.0%
! AAGAGGGTTTGTGTCTTTTG+TGG + Chr1:64321980-64321999 MsG0180003568.01.T01:CDS 40.0%
! ATGGTGGAAGACATGTCATA+GGG + Chr1:64322559-64322578 MsG0180003568.01.T01:intron 40.0%
! CTGAAGAGTATGACATCTCA+AGG + Chr1:64324759-64324778 MsG0180003568.01.T01:CDS 40.0%
! CTTTTCTCACATACCTCTCT+AGG - Chr1:64322737-64322756 None:intergenic 40.0%
! GATGCTTTTATTGCCTTACC+AGG + Chr1:64323247-64323266 MsG0180003568.01.T01:CDS 40.0%
! GGTGTACTATTCTTTAGCTC+AGG - Chr1:64323277-64323296 None:intergenic 40.0%
! GTGGGACTAGTAAATGTTGA+TGG + Chr1:64324451-64324470 MsG0180003568.01.T01:CDS 40.0%
! TTTTGTGGATGGGGTGAAAA+TGG + Chr1:64322142-64322161 MsG0180003568.01.T01:intron 40.0%
!! CCCTATTCTGTTTTTCTTCC+TGG + Chr1:64324867-64324886 MsG0180003568.01.T01:three_prime_UTR 40.0%
!! GAAGCATTGGTCTAATGGTT+CGG + Chr1:64322518-64322537 MsG0180003568.01.T01:intron 40.0%
!! GACCATTGGTTTAGATCGAA+GGG + Chr1:64324118-64324137 MsG0180003568.01.T01:intron 40.0%
!! TGACCATTGGTTTAGATCGA+AGG + Chr1:64324117-64324136 MsG0180003568.01.T01:intron 40.0%
ACAATTATGTGGCGCGCATT+TGG - Chr1:64324535-64324554 None:intergenic 45.0%
AGTGACTGTTACAGAAGGCA+AGG + Chr1:64323929-64323948 MsG0180003568.01.T01:intron 45.0%
ATCGACAAAGCTGTGGAAGA+GGG + Chr1:64324499-64324518 MsG0180003568.01.T01:CDS 45.0%
CAAGATAGAGGGAAAGAGAG+AGG + Chr1:64323996-64324015 MsG0180003568.01.T01:intron 45.0%
CAAGATGCTGCCATTGAACT+TGG + Chr1:64322031-64322050 MsG0180003568.01.T01:CDS 45.0%
CTCTTTCCCTCTATCTTGCA+AGG - Chr1:64323994-64324013 None:intergenic 45.0%
GAAAGAGAGAGGGTGATAAG+GGG + Chr1:64324007-64324026 MsG0180003568.01.T01:intron 45.0%
GACCCTTCGATCTAAACCAA+TGG - Chr1:64324123-64324142 None:intergenic 45.0%
GACTTCAAGAAGCTCCTCTA+GGG - Chr1:64324295-64324314 None:intergenic 45.0%
GAGGTGGAAAGAAGCTATTG+TGG + Chr1:64324839-64324858 MsG0180003568.01.T01:three_prime_UTR 45.0%
GCCAAATATGTTAGGGTCTC+AGG - Chr1:64323652-64323671 None:intergenic 45.0%
GCCTGAGACCCTAACATATT+TGG + Chr1:64323648-64323667 MsG0180003568.01.T01:intron 45.0%
GGAAAGAGAGAGGGTGATAA+GGG + Chr1:64324006-64324025 MsG0180003568.01.T01:intron 45.0%
TCACAAGCTGTTCATGATGG+TGG + Chr1:64322543-64322562 MsG0180003568.01.T01:intron 45.0%
TCATAACCTGGGCTCAACTT+GGG + Chr1:64324313-64324332 MsG0180003568.01.T01:CDS 45.0%
TGACTTCAAGAAGCTCCTCT+AGG - Chr1:64324296-64324315 None:intergenic 45.0%
TTTGACATTCCACAACTCGC+AGG + Chr1:64324429-64324448 MsG0180003568.01.T01:intron 45.0%
! GGATCCTTTTTGCAGCTAAC+TGG + Chr1:64323154-64323173 MsG0180003568.01.T01:intron 45.0%
! GTCTTTTGTGGAAGTAGTCC+TGG + Chr1:64321992-64322011 MsG0180003568.01.T01:CDS 45.0%
! GTTTATGGAGGAGGAAGCAT+TGG + Chr1:64322505-64322524 MsG0180003568.01.T01:CDS 45.0%
! TTCTTTAGCTCAGGCTAACC+TGG - Chr1:64323268-64323287 None:intergenic 45.0%
!! AGGAGGAAGCATTGGTCTAA+TGG + Chr1:64322513-64322532 MsG0180003568.01.T01:CDS 45.0%
!! GATGGTGGAAGACATGTCAT+AGG + Chr1:64322558-64322577 MsG0180003568.01.T01:intron 45.0%
!!! AAATATAAATTTTTTGTTTT+TGG + Chr1:64322290-64322309 MsG0180003568.01.T01:intron 5.0%
!!! TTTTTTTTTTTATATATCTA+TGG + Chr1:64322368-64322387 MsG0180003568.01.T01:intron 5.0%
ACATTCCACAACTCGCAGGT+GGG + Chr1:64324433-64324452 MsG0180003568.01.T01:intron 50.0%
CAAGACACTCATGCCTAGAG+AGG + Chr1:64322721-64322740 MsG0180003568.01.T01:CDS 50.0%
CAGCTAACTGGTGAAACGGT+GGG + Chr1:64323166-64323185 MsG0180003568.01.T01:intron 50.0%
CATCGACAAAGCTGTGGAAG+AGG + Chr1:64324498-64324517 MsG0180003568.01.T01:CDS 50.0%
CTCTCTAGGCATGAGTGTCT+TGG - Chr1:64322723-64322742 None:intergenic 50.0%
GCATCAAAGGAAGGCAGAGA+TGG + Chr1:64323213-64323232 MsG0180003568.01.T01:CDS 50.0%
GGGAAAGAGAGAGGGTGATA+AGG + Chr1:64324005-64324024 MsG0180003568.01.T01:intron 50.0%
GGGATCCCTTGCAAGATAGA+GGG + Chr1:64323985-64324004 MsG0180003568.01.T01:intron 50.0%
GTCATAACCTGGGCTCAACT+TGG + Chr1:64324312-64324331 MsG0180003568.01.T01:CDS 50.0%
TAGCTCAGGCTAACCTGGTA+AGG - Chr1:64323263-64323282 None:intergenic 50.0%
TGGGATCCCTTGCAAGATAG+AGG + Chr1:64323984-64324003 MsG0180003568.01.T01:intron 50.0%
TTAGGGTCTCAGGCAAAGTC+AGG - Chr1:64323642-64323661 None:intergenic 50.0%
TTCGAAGTTGAGCTGGCAGA+TGG + Chr1:64324718-64324737 MsG0180003568.01.T01:CDS 50.0%
! ATGAATCCCAAGTTGAGCCC+AGG - Chr1:64324322-64324341 None:intergenic 50.0%
AGGTGGTTATGGGACCCTAG+AGG + Chr1:64324278-64324297 MsG0180003568.01.T01:intron 55.0%
GAAGCAACGCCTTCGTGACA+AGG - Chr1:64324701-64324720 None:intergenic 55.0%
GACATTCCACAACTCGCAGG+TGG + Chr1:64324432-64324451 MsG0180003568.01.T01:intron 55.0%
GCAGCTAACTGGTGAAACGG+TGG + Chr1:64323165-64323184 MsG0180003568.01.T01:intron 55.0%
GCGTTGCTTCGAAGTTGAGC+TGG + Chr1:64324711-64324730 MsG0180003568.01.T01:CDS 55.0%
CTAGTCCCACCTGCGAGTTG+TGG - Chr1:64324441-64324460 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 64321706 64325073 64321706 ID=MsG0180003568.01;Name=MsG0180003568.01
Chr1 mRNA 64321706 64325073 64321706 ID=MsG0180003568.01.T01;Parent=MsG0180003568.01;Name=MsG0180003568.01.T01;_AED=0.50;_eAED=0.50;_QI=238|0.83|0.85|1|0.5|0.57|7|289|203
Chr1 exon 64321706 64322063 64321706 ID=MsG0180003568.01.T01:exon:23765;Parent=MsG0180003568.01.T01
Chr1 exon 64322484 64322534 64322484 ID=MsG0180003568.01.T01:exon:23766;Parent=MsG0180003568.01.T01
Chr1 exon 64322713 64322742 64322713 ID=MsG0180003568.01.T01:exon:23767;Parent=MsG0180003568.01.T01
Chr1 exon 64323169 64323268 64323169 ID=MsG0180003568.01.T01:exon:23768;Parent=MsG0180003568.01.T01
Chr1 exon 64324280 64324350 64324280 ID=MsG0180003568.01.T01:exon:23769;Parent=MsG0180003568.01.T01
Chr1 exon 64324451 64324594 64324451 ID=MsG0180003568.01.T01:exon:23770;Parent=MsG0180003568.01.T01
Chr1 five_prime_UTR 64321706 64321943 64321706 ID=MsG0180003568.01.T01:five_prime_utr;Parent=MsG0180003568.01.T01
Chr1 CDS 64321944 64322063 64321944 ID=MsG0180003568.01.T01:cds;Parent=MsG0180003568.01.T01
Chr1 CDS 64322484 64322534 64322484 ID=MsG0180003568.01.T01:cds;Parent=MsG0180003568.01.T01
Chr1 CDS 64322713 64322742 64322713 ID=MsG0180003568.01.T01:cds;Parent=MsG0180003568.01.T01
Chr1 CDS 64323169 64323268 64323169 ID=MsG0180003568.01.T01:cds;Parent=MsG0180003568.01.T01
Chr1 CDS 64324280 64324350 64324280 ID=MsG0180003568.01.T01:cds;Parent=MsG0180003568.01.T01
Chr1 CDS 64324451 64324594 64324451 ID=MsG0180003568.01.T01:cds;Parent=MsG0180003568.01.T01
Chr1 CDS 64324689 64324784 64324689 ID=MsG0180003568.01.T01:cds;Parent=MsG0180003568.01.T01
Chr1 three_prime_UTR 64324785 64325073 64324785 ID=MsG0180003568.01.T01:three_prime_utr;Parent=MsG0180003568.01.T01
Chr1 mRNA 64324430 64325073 64324430 ID=MsG0180003568.01.T02;Parent=MsG0180003568.01;Name=MsG0180003568.01.T02;_AED=0.50;_eAED=0.50;_QI=0|1|0.5|1|1|1|2|289|86
Chr1 exon 64324430 64324594 64324430 ID=MsG0180003568.01.T02:exon:23772;Parent=MsG0180003568.01.T02
Chr1 CDS 64324430 64324594 64324430 ID=MsG0180003568.01.T02:cds;Parent=MsG0180003568.01.T02
Chr1 CDS 64324689 64324784 64324689 ID=MsG0180003568.01.T02:cds;Parent=MsG0180003568.01.T02
Chr1 three_prime_UTR 64324785 64325073 64324785 ID=MsG0180003568.01.T02:three_prime_utr;Parent=MsG0180003568.01.T02
Gene Sequence

>MsG0180003568.01.T01

ATGGAGACAAATGGTGAGATAAGAGTGTCAAAGTTCAAGAGGGTTTGTGTCTTTTGTGGAAGTAGTCCTGGCAAGAAAAGTAGTTATCAAGATGCTGCCATTGAACTTGGAAATGAATTGGTGTCAAGAAATATTGATCTAGTTTATGGAGGAGGAAGCATTGGTCTAATGAGTTATTCCAAGACACTCATGCCTAGAGAGCTAACTGGTGAAACGGTGGGAGAAGTAAAAGCTGTAGCAGATATGCATCAAAGGAAGGCAGAGATGGCTAAGCATTCAGATGCTTTTATTGCCTTACCAGGTGGTTATGGGACCCTAGAGGAGCTTCTTGAAGTCATAACCTGGGCTCAACTTGGGATTCATGACAAACCGGTGGGACTAGTAAATGTTGATGGATACTTTAATTCATTACTTTCATTCATCGACAAAGCTGTGGAAGAGGGATTTATCAGTCCAAATGCGCGCCACATAATTGTATCAGCACCTACATCAAAAGAGTTAGTGAAGAAACTGGAGGATTATGTTCCTTGTCACGAAGGCGTTGCTTCGAAGTTGAGCTGGCAGATGGAACAAGAGCTAGCCTATCCTGAAGAGTATGACATCTCAAGGTAG

>MsG0180003568.01.T02

TTGACATTCCACAACTCGCAGGTGGGACTAGTAAATGTTGATGGATACTTTAATTCATTACTTTCATTCATCGACAAAGCTGTGGAAGAGGGATTTATCAGTCCAAATGCGCGCCACATAATTGTATCAGCACCTACATCAAAAGAGTTAGTGAAGAAACTGGAGGATTATGTTCCTTGTCACGAAGGCGTTGCTTCGAAGTTGAGCTGGCAGATGGAACAAGAGCTAGCCTATCCTGAAGAGTATGACATCTCAAGGTAG
Protein sequence

>MsG0180003568.01.T01

METNGEIRVSKFKRVCVFCGSSPGKKSSYQDAAIELGNELVSRNIDLVYGGGSIGLMSYSKTLMPRELTGETVGEVKAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVITWAQLGIHDKPVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTSKELVKKLEDYVPCHEGVASKLSWQMEQELAYPEEYDISR*

>MsG0180003568.01.T02

LTFHNSQVGLVNVDGYFNSLLSFIDKAVEEGFISPNARHIIVSAPTSKELVKKLEDYVPCHEGVASKLSWQMEQELAYPEEYDISR*